CN110236980A - A kind of moisturizer and preparation method and application containing tremella polysaccharides - Google Patents
A kind of moisturizer and preparation method and application containing tremella polysaccharides Download PDFInfo
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- CN110236980A CN110236980A CN201910568795.9A CN201910568795A CN110236980A CN 110236980 A CN110236980 A CN 110236980A CN 201910568795 A CN201910568795 A CN 201910568795A CN 110236980 A CN110236980 A CN 110236980A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention discloses a kind of moisturizer containing tremella polysaccharides and preparation method and application, the method is with the production of tremella polysaccharides moisturizer as main component.It is made of following components in parts by weight: 20-40 parts of tremella polysaccharides, 10-20 parts of oligomerization hyaluronic acid, 5-10 parts of L- rhamnose, 3-5 parts of D-pantothenyl aleohol, 25-62 parts of deionized water.A kind of moisturizer containing tremella polysaccharides provided by the invention, can effectively improve the moisture content in skin, improve and protect skin barrier; and the composition has good film forming characteristics; there is the effect of collaboration moisturizing, does not contain chemical industry additive, be suitble to various crowd's skin care moisturizings.
Description
Technical field
The invention belongs to the technical fields of cosmetics, and in particular to a kind of moisturizer and preparation method containing tremella polysaccharides with
Using.
Background technique
As the improvement of people's living standards, people increasingly pay attention to the nursing and maintenance to autologous skin.The skin of health
Skin should be it is smooth moisten flexible, and this depends primarily on the moisture content in skin.Moisturizing is the pass of skin nursing
Key, moisture-retention function of skin decline will lead to a variety of skin problems, and natural in normal and flexible skin and human skin
Moisture-keeping system have close relationship.Due to the growth at age and the influence of external environment, the moisturizing mechanism of skin is damaged
Wound, skin tissue cell and intercellular moisture content are reduced, and cause cell arrangement close, and angle is worked as in collagen dehydration hardening
When moisture drops to 10% or less in matter layer, skin, which will seem, to be dried, follows the string, wrinkling, and skin aging process accelerates.Cause
This, moisture is most important to skin health, and water conservation moisturizing is always one of most important research topic of skin protection cosmetics.
Moisturizing class cosmetics mainly play effect by moisturizer, moisturizer to simulate in human skin by
The natural moisture preserving system of oil, water, NMF composition, effect are to delay loss of moist, increase dermal-epidermal moisture penetration, are skin
Skin temporarily provides protection, reduces damage, promotes repair process.Traditional moisturizer mainly has glycerol, propylene glycol, butanediol, sorb
Alcohol, pyrrolidone sodium carboxylate, polyethylene glycol, sodium lactate etc., they are by being chemically synthesized, in addition to pyrrolidone sodium carboxylate,
Other are still now widely used cosmetic humectant.They all have the advantages that performance of keeping humidity is good, and inexpensive easy
?.But moisturizer made of chemistry easily causes the malaise symptoms such as allergy.
Polysaccharide moisturizer, due to it has no toxic side effect, with the characteristics such as the affinity of skin is good, become cosmetics moisturizing
One new hot spot of research and development of agent.The moisture-keeping function of polysaccharide is: the 1. hydroxyl in polymolecular, carboxyl and other polar groups
Group can form hydrogen bond with hydrone and combine a large amount of moisture, while polysaccharide molecule chain is also interweaved and reticulates, in addition with water
Hydrogenbond, play very strong water retention.In addition, in extracellular matrix, other polysaccharide components and fibre of polysaccharide and skin
Dimension shape protein collectively constitutes the extracellular gelatinised matrix containing large quantity of moisture, provides moisture for skin.2. polysaccharide have it is good at
Film properties can form one layer of uniform film in skin surface, the moisture evaporation of skin surface be reduced, so that moisture is from basis set
It knits and permeates into cuticula, induce the further aquation of cuticula.The moisture of skin itself is saved, and completes moisturizing effect.Therefore,
The high-hydroscopicity of polysaccharide and good film forming perfect combination, can provide good moistening effect for skin.Therefore, polysaccharide is made
It is applied to cosmetics for moisturizer, meets demand of the people to high-quality cosmetics, the development for becoming moisturizing cosmetic becomes
Gesture.
Tremella also known as tremella are a kind of higher funguses, and nature and flavor are sweet, flat, have the effect of nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid.
From the Shennong's Herbal of China's Han dynasty, to Ming Dynasty's Compendium of Material Medica and modern age " Dictionary of Chinese Pharmacy " to tremella medicine
With the effect of all made record.Wherein the main active of tremella is tremella polysaccharides, thin from Tremella fructification and tremella
It separated in born of the same parents' submerged fermentation spore, purify obtained heteroglycan.Its main chain is that the sweet dew being made of α-(1-3)-glycosidic bond gathers
Sugar, the 2 of main chain, it is connected with the side chain of the residues such as glucose, xylose, fucose and common uronic acid composition on 4,6, lives
Property center is this common structure part of α-(1-3)-mannosan.According to the molecular characterization of tremella polysaccharides, tremella is more
Sugar has preferable moisture-keeping functions, and the application in cosmetics has broad prospects.
In " extraction of tremella polysaccharides and its beauty functions research " paper that Ren Qing, Li Shoumian, Li Lina etc. are delivered, benefit
Tremella polysaccharides are extracted from tremella dry product with Hot water extraction and propose tremella polysaccharides, and pass through crystal violet method, in vivo survey hydration
Rate and moisture loss rate method and external moisture loss rate method of surveying prove that tremella polysaccharides have preferable moisture-retaining capacity and certain
Oxidation resistance can be used in cosmetics.Come the lucky, fourth of the twelve Earthly Branches He Congfen, Zhao Jin, Dong Yin delivered " tremella polysaccharides industrialization mention
Taking technique optimization and skin-care effect research " in paper, propose that tremella polysaccharides product has preferable thermal stability and ph stability
Property, suitable for the cosmetics of all kinds of pH ranges, while guaranteeing stability of the product in longer storage, skin-care effect detection
Show that tremella polysaccharides product has excellent moisture-keeping efficacy, dermatoglyph degree can be improved, reduce skin roughness, increases skin
Elasticity can be used as functional component additive application in cosmetics.
The Chinese patent literature of publication No. CN 106880513A (application number 201710220943.9) discloses a kind of biology
Polysaccharide moisturizer and its preparing the application in cosmetics；Publication No. CN 106963665A (application number 201710220950.9)
Chinese patent literature disclose one group of four-dimension moisturizer and its application in preparation moisturizing cosmetic, use in patent
The moisturizer that 17 kinds of different moisturizer are compounded, wherein most are polysaccharide moisturizing molecule, and it is solidifying to greatly improve it
Colloidality energy makes it be added in cosmetics the rheological characteristic that can change its script.And the moisture of skin of the embodiment provided in invention
Percutaneous value of scattering and disappearing is higher, illustrates that the lost ability of its reduction moisture of skin is weaker, is unfavorable for the abundant moisturizing of skin.
Summary of the invention
To solve the above-mentioned problems, the purpose of the present invention is to provide a kind of moisturizer containing tremella polysaccharides, tremella polysaccharides
Because it has good performance of keeping humidity, the synergistic strong moisturizing also generated with oligomerization hyaluronic acid, L- rhamnose, D-pantothenyl aleohol
Effect.The extraction process yield of tremella polysaccharides involved in the present invention is high, and average recoveries 40.57%, production cost is low,
There are also its natural environmental-protective compared with the moisturizer made of the traditional chemical, to the advantage of skin safe fanout free region, the invention further relates to
The preparation method of the moisturizer and the application in cosmetics.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of moisturizer containing tremella polysaccharides, is made by following components in parts by weight: 20-40 parts of tremella polysaccharides, oligomerization are saturating
10-20 parts of bright matter acid, 5-10 parts of L- rhamnose, 3-5 parts of D-pantothenyl aleohol and 25-62 parts of deionized water；Each component parts by weight summation is
100 parts.
The invention also includes a kind of preparation methods of moisturizer containing tremella polysaccharides, comprising the following steps:
(1) under room temperature, the tremella polysaccharides of 20-40 parts by weight and the oligomerization hyaluronic acid of 10-20 parts by weight is weighed, 30- is used
The deionized water dissolving of 40 parts by weight, mixing make it dissolve；
(2) the L- rhamnose of 5-10 parts by weight and the D-pantothenyl aleohol of 3-5 parts by weight are added by weight in step B1 solution,
It is added and is uniformly mixed while stirring at 120r/min；
(3) deionized water of 60-70 parts by weight is added to get the moisturizer containing tremella polysaccharides.
Further, the preparation method of the tremella polysaccharides, comprising the following steps:
(1) 10 parts by weight Tremella fructifications are weighed to mix with the deionized water of 500 parts by weight, are used after 80 DEG C of mashing 7min
200 mesh filter-cloth filterings collect filtrate, the dehydrated alcohol of 3 times of volumes of filtrate are added, for 24 hours, centrifuging and taking precipitating is drying to obtain alcohol precipitation
Tremella Thick many candies；
(2) refine: the tremella Thick many candies for taking the 10 parts by weight step (1) of deionized water dissolving of 100 parts by weight to prepare obtain
Tremella Thick many candies solution is added and the isometric Sevage reagent of tremella Thick many candies solution, after sufficiently vibrating 20min, 5000r/
It is centrifuged 15min under conditions of min, collects upper layer polysaccharide solution, then addition is isometric with polysaccharide solution into upper layer polysaccharide solution
Sevage reagent repeats the above steps 9 times；
(3) it dialyses: the polysaccharide solution after step (2) removing protein is put into the bag filter that 44mm molecular cut off is 3000
In, 4 DEG C of deionised waters are dialysed 48h, and every 6h changes a deionized water, the anhydrous second of 3 times of volumes of polysaccharide solution of dialysis is added
Alcohol precipitating filters to obtain precipitating, and finally freeze-drying precipitates to obtain tremella polysaccharides.
The invention also includes a kind of cosmetics of moisturizer containing tremella polysaccharides, are made of following weight parts component: moisturizer
15-28 parts, 940 0.5 parts of card wave, 0.1-0.5 parts of arginine, 0.1-1 parts of preservative, 70-85 parts of deionized water, each component weight
Measuring part summation is 100 parts.
The invention also includes a kind of preparation methods of the cosmetics of moisturizer containing tremella polysaccharides, comprising the following steps:
(1) under room temperature, in dust proof workshop, the tremella polysaccharides moisturizer of 15-28 parts by weight, the card of 0.5 parts by weight are weighed
Wave nurse 940, the arginine of 0.1-0.5 parts by weight, in reaction kettle, mixing makes it dissolve the preservative of 0.1-1 parts by weight,
Ultraviolet disinfection disinfectant measure is carried out, referred to as A phase is spare；
(2) deionized water that 70-85 parts by weight are added in uniform solution is mixed toward A, is mixed at 100r/min equal
It is even, ultraviolet disinfection disinfectant measure is carried out to get a kind of cosmetics of moisturizer containing tremella polysaccharides.
Beneficial effects of the present invention:
1, moisturizer of the invention, is made using tremella polysaccharides of primary raw material, and can in a short time to skin into
The potent moisturizing of row, water conservation, the reparation of water lock, are effectively improved and protect skin barrier.
2, the tremella polysaccharides added in the present invention, are a kind of water-soluble high molecular polymerizations extracted from tremella
Object and plant extract the polysaccharide that unique molecular weight in hyaluronic acid crosses million, have good lubricity.It can inhibit
The peroxidization of cytolipin, to prevent the aging of skin；Promote elastic cell's growth；Reduce injury of the UV to skin；
Epidermal cell is activated, light loss skin is repaired；Accelerate skin regeneration；Wrinkle-chasing goes to wrinkle, and can form one layer in skin surface uniformly has
Certain thickness film makes skin have good smooth feeling and moist feeling, does not shrink, does not feel tight, plays guarantor to skin
Shield effect.The present invention has the characteristics that polysaccharide moisturizing using it, is applied in moisturizer, also with oligomerization hyaluronic acid, L-
Rhamnose, D-pantothenyl aleohol are compounded, and the synergistic moistening effect of generation keeps the moistening effect of its moisturizer more lasting,
Moisture pick-up properties is more preferable.
3, the present invention does not contain chemical stimulating compositions, and can releive skin, is used for a long time without sensitization symptom.
4, the polysaccharide extracting process contained in the present invention, remains the active constituent in tremella polysaccharides, no to the greatest extent
It is destroyed by reaction conditions such as high temperature.
5, the extraction process of tremella polysaccharides, quickly and easily, and yield is high, and economic cost is few, and it is raw to be suitable for industrial-scale
It produces, there is extensive market popularization value, social and economic benefit is significant.
Detailed description of the invention
Fig. 1 is the test result of Examples 1 to 3 and control group MMV increased percentage value.
Fig. 2 is the test result of Examples 1 to 3 and control group TEWL increased percentage value.
Specific embodiment
In order to better understand the present invention, below by embodiment to the present invention into further explanation, embodiment is served only for
It explains the present invention, any restriction can't be constituted to the present invention.
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Embodiment 1
A kind of moisturizer containing tremella polysaccharides, raw material calculates by weight, including following components: 25 parts of tremella polysaccharides, few
12 parts of poly- hyaluronic acid, 8 parts of L- rhamnose, 3 parts of D-pantothenyl aleohol, 52 parts of deionized water.
Preparation method:
(1) under room temperature, the tremella polysaccharides and 12 parts by weight oligomerization hyaluronic acids of 25 parts by weight are weighed, with 30 parts by weight go from
Sub- water dissolution, mixing make it dissolve；
(2) then, 8 parts by weight L- rhamnoses and 3 parts by weight D-pantothenyl aleohols are added thereto, are stirred below in 120r/min
Side is added and is uniformly mixed；
(3) it is filled with 22 parts by weight of deionized water to get a kind of moisturizer containing tremella polysaccharides.
Embodiment 2
A kind of moisturizer containing tremella polysaccharides, raw material is calculated in parts by mass, including following components: 20 parts of tremella polysaccharides, few
10 parts of poly- hyaluronic acid, 5 parts of L- rhamnose, 3 parts of D-pantothenyl aleohol, 62 parts of deionized water.
Experimental method is the same as embodiment 1.
Embodiment 3
A kind of moisturizer containing tremella polysaccharides, raw material is calculated in parts by mass, including following components: 40 parts of tremella polysaccharides, few
20 parts of poly- hyaluronic acid, 10 parts of L- rhamnose, 5 parts of D-pantothenyl aleohol, 25 parts of deionized water.
Experimental method is the same as embodiment 1.
Efficacy assessments
1. physical and chemical index is assessed
Character: tremella polysaccharides moisturizer prepared by embodiment 1 to 3 is homogeneous, there is light plant fragrance, transparent mucus
Shape.Packing is sealed after product is sterilized, room temperature preservation is not mouldy after three months, free from extraneous odour, composition transparent and stable.
PH value inspection: 1 to 3 moisturizer of Example is measured with pH meter respectively in 25 DEG C, and it is weak for measuring pH value
Between sour 5.2-6.5.
Low temperature resistant test: 1 to 3 moisturizer of embodiment being loaded in container respectively and is sealed, and is kept in -4 DEG C of refrigerators for 24 hours, extensive
It is preceding without Traits change with test after multiple room temperature.
Heat resistant test: 1 to 3 moisturizer of embodiment being loaded in container respectively and is sealed, and is kept for 24 hours in 40 DEG C of insulating boxs,
Phenomenon is discharged without condensate oil after restoring room temperature.
Centrifugal test: being respectively placed in 3500r/min in centrifuge for 1 to 3 moisturizer of embodiment, is centrifuged 30min, nothing point
Layer phenomenon.
Packing is sealed after 1 to 3 moisturizer of embodiment is sterilized separately, room temperature preservation is not mouldy after three months, is no different
Taste, it is believed that the moisturizer transparent and stable.
2. the measuring of hydroscopicity:
2.1 test methods: moisturizer prepared by embodiment 1, by the hydroscopicity of gravimetric detemination moisturizer, by following step
It is rapid to carry out:
(1) prepare before test: in experiment proxima luce (prox. luc), placing the saturation potassium nitrate solution of certain volume at drier bottom, make
Liquid depth is greater than 1cm, places porous barrier thereon, closes the lid, and keeps sealing state, is placed in 25 degrees Celsius of environment.
After the superjacent saturation of the air, that is, it can reach and maintain 94% relative humidity.
(2) 0.3% micromolecule hyaluronic acid sodium water solution is prepared as control sample.
(3) it product testing: selects the glass plate of 5*5cm to clean drying, and sticks the medical adhesive tape of 3M.Weigh 0.1g's
It is uniformly applied on 3M adhesive tape by the micromolecule hyaluronic acid sodium water solution of moisturizer and 0.1g 0.3%, and dosage is about
2mg/cm2, weighing quality is W1.Then the glass plate after smearing is placed on the porous barrier of simple humidistat, and guarantee every
The circulation of plate air up and down, (6h, 12h, for 24 hours, 36h) takes out rapidly after placing a period of time, is weighed as W2, real three times in parallel
It tests, is averaged.
(4) final result is presented with hydroscopicity, measures the formula of hydroscopicity: Rr (%)=(quality W2- sample after sample moisture absorption
Quality W1 before product moisture absorption) quality W1 before/sample moisture absorption.
2.2 experimental datas are shown in Table 1
The hydroscopicity of 1 tremella polysaccharides moisturizer of table compares
Sodium Hyaluronate is a kind of glutinous sugar of bionical object, its theoretical water retention value, which reaches 1000ml/g, to be retained in human skin
Key component, be the good moisturizer of a kind of moistening effect.It can be obtained from above data, the hydroscopicity of tremella polysaccharides composition
It is more somewhat higher than micromolecule hyaluronic acid sodium hydroscopicity, it can illustrate that the moistening effect of composition is good.
3. the measuring of percentage of water loss
3.1 test methods: moisturizer prepared by embodiment 1, by the percentage of water loss of gravimetric detemination moisturizer, by following
Step carries out:
(1) prepare before test: gravimetric detemination moisturizer percentage of water loss must carry out in a constant temperature, constant humidity environment.With test
Proxima luce (prox. luc) places the unsaturated carbonate potassium solution of certain volume in drier bottom, liquid depth is made to be greater than 1cm, places thereon more
Hole partition.It closes the lid, keeps closed state, be placed in 25 degrees Celsius of environment, after water vapor in air partial pressure is constant,
It can maintain about 43% relative humidity.
(2) 0.3% micromolecule hyaluronic acid sodium water solution is prepared as control sample.
(3) it product testing: selects the glass plate of 5*5cm to clean drying, and sticks the medical adhesive tape of 3M.Weigh 0.1g's
It is uniformly applied on 3M adhesive tape by the micromolecule hyaluronic acid sodium water solution of tremella polysaccharides moisturizer and 0.1g 0.3%,
Dosage is about 2mg/cm2, weighing quality is M1.Then the glass plate after smearing is placed on the porous barrier of simple humidistat,
And guarantee the circulation of partition air up and down, (6h, 12h, for 24 hours, 36h) takes out rapidly, is weighed as M2 after placing a period of time, flat
Row is tested three times, is averaged.
(4) final result is presented with percentage of water loss, the formula of gravimetric detemination percentage of water loss: (%)=(quality after sample moisture absorption
Quality M2 before M1- sample moisture absorption) quality M1 before/sample moisture absorption
1.2 experimental datas are shown in Table 2
The comparison of 2 tremella polysaccharides moisturizer percentage of water loss of table
From above-mentioned experimental result, the dehydration ability of the tremella polysaccharides moisturizer in the present invention with small point of 0.3%
Sub- aqueous solution of sodium hyaluronate is substantially similar.
4. skin irritation test
4.1 test purpose
According to skin irritation/corrosion test in 2015 editions " cosmetics safety technical specification ", determines and evaluate this
Tremella polysaccharides moisturizer in invention is to the whether irritating effect in mammal skin part or corrosiveness and its degree.
4.2 test method
(1) 4 adults, health, the white rabbit of skin zero damage are chosen and is used as experimental animal, female and male,
But jenny should be unpregnancy and not farrow.Experimental animal answers single cage to raise, and animal will be in experimental animal room ring before testing
3 day time is at least adapted in border.Experimental animal and experimental animal room should meet national respective specified.Selection standard mixed feed,
Drinking-water does not limit.
(2) before test about for 24 hours, experimental animal back backbone diamond wool is cut, can not damages epidermis, unhairing range is left,
Right each about 3cm × 3cm.
(3) tested material about 0.5mL (g) is taken to be coated directly on skin, then with two layers of gauze (2.5cm × 2.5cm) and one
Layer glassine paper covering, then fixed with nonirritant adhesive plaster and bandage.Other side skin is as control., it smears 1 time daily,
It is continuous to smear 14 days.Since second day, cropping is answered before smearing every time, remains tested material with water or nonirritant solvent cleaning.
Observation after one hour by table 3 as a result, score, check plot and trial zone are equally handled.
4.3 evaluation of result: being calculated according to the following formula daily every animal average integral, determines skin irritatin intensity with table 4.
Daily every animal average integral=(∑ erythema and oedema integral/animal subject number)/14
The scoring of 3 skin wound repair of table
| Dermoreaction | Integral |
| Erythema and eschar are formed | |
| Without erythema | 0 |
| Slight erythema (visible reluctantly) | 1 |
| Obvious erythema | 2 |
| Moderate-severe erythema | 3 |
| Severe erythema (aubergine) to slight eschar is formed | 4 |
| Oedema is formed | |
| Without oedema | 0 |
| Slight oedema (visible reluctantly) | 1 |
| Mild edema (cutaneous protuberance is clearly outlined) | 2 |
| Intermediate edema (cutaneous protuberance about 1mm) | 3 |
| Severe edema (cutaneous protuberance is more than 1mm, and range expands) | 4 |
| Top | 8 |
4 skin irritatin strength grading of table
| Integral mean value | Intensity |
| 0~< 0.5 | It is nonirritant |
| 0.5~< 2 | Subexcite |
| 2.0~< 6.0 | Moderate stimulation |
| 6.0~8.0 | Strong and stimulating |
4.4 experimental result
The average integral of tremella polysaccharides moisturizer embodiment 1,2,3 in the present invention is respectively 0.12,0.15,0.13, can
Think that the moisturizer to no skin irritation, can carry out the measurement of body water content.
5. skin moisture content MMV test method
5.1 test philosophy
The moisture-keeping efficacy of cosmetics prepared by cosmetic formulations system is tested using CM825 type skin oil-water soda acid
Instrument, " measurement of cosmetics the moisture-keeping efficacy "-MMV (meanmoisturevalue) drafted by units such as Beijing Technology and Business Universitys
What method was measured.The method using capacitance method test human body keratoderma moisture content, its principle be based on water and
The difference in dielectric constant of other substances is significant, and according to moisture content of skin difference, the capacitance for measuring skin is different, observation ginseng
Number can represent skin moisture value.
5.2 experimental conditions and volunteer require
Test environment conditions: test environment temperature is 22 ± 1 DEG C, and humidity is 50 ± 5%, and carries out dynamic in real time and examine
It surveys；
Volunteer requires: effective volunteer at least 30 people, the age, (gestation or breast feeding women removed between 16-65 years old
Outside)；Forearm test zone capacitance method moisture of skin measures the basic value of meaning between 15-100；Without serious systemic disease, nothing
Immune deficiency or autoimmune disease person；No active anaphylactic disease person；Previously to skin-care cosmetics without severe allergy history
Person；Hormone medicine and immunosuppressor person are not used in nearly one month；It is not other clinical test persons of participation；By regulation
Use test medicine and all information；All volunteers should fill in informed consent form before testing.
Prepare before 5.3 tests
Any product (cosmetics or topical drug) cannot be used within 2-3 days before recipient site.Before experiment, subject needs same
It is surveyed in meaning cleaning both hands forearm, with dry face tissue wiped clean.It is surveyed in subject's both hands forearm after cleaning and carries out measured zone
Label.At least 30min that sits quietly in room should be being complied with standard before official testing, cannot drunk water, forearm exposure is in test mode
It places, keeps loosening.
5.4 testing procedure
3 × 3cm of label on the inside of left and right arm in experiment2Resistance to test area, same arm can mark multiple regions, region
It is spaced 1cm.Test product and blank control are randomly dispersed in left and right arm.Using capacitance method skin analyzer carry out by
Try the measurement in region and control zone.Each region is according to parallel determination 15 times.The plain pape of each test zone is first measured, so
2.0 ± 0.1mg sample/cm is pressed afterwards2Dosage, test is spread evenly across in trial zone using latex finger cot.Smearing is surveyed respectively
The moisture content of skin (being measured when verifying by this time) in amount 30min, 60min, 120min, tested region and blank control region,
The test of the same volunteer is completed by the same survey crew.
5.5 test data
Measure the MMV value of each period respectively by experiment.
Calculation formula are as follows: W=((W1-W0)/W0× 100%
Note: W%=skin moisture content increased percentage (MMV)
W0=skin uses moisture content before sample
W1=skin uses moisture content after sample
The proportion of the moisture-retention gel water embodiment 1-3 and comparative example 1-2 of 5.6 moisturizer containing tremella polysaccharides is as shown in table 5:
5 embodiment of table and comparative example moisturizing cosmetic proportion
5.7 preparation methods:
(1) A phase: component 9 is added in component 2, is dissolved by heating, and is heated to 80 DEG C of dissolutions, is cooled to 40 DEG C, is added
Component 2 is added in stirring, sufficiently dissolves for 1,3,4,5,6,7,8 stirring and dissolving of component.
(2) B phase: component 11,12 is added in suitable container and is heated to 40 DEG C of dissolutions.
(3) B is added to A phase, is slowly stirred until homogeneous transparent, stirred at low speed and component 10 is added, be stirred until homogeneous,
It is eventually adding component 13 to stir evenly, obtains opaque products.
5.8 measure the result such as table 6 of MMV value:
The MMV increased percentage value of each period of table 6
| Time/min | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 |
| 0 | 0 | 0 | 0 | 0 | 0 |
| 30 | 146.23% | 92.56% | 122.78% | 71.89% | 78.56% |
| 60 | 131.52% | 87.45% | 115.66% | 63.14% | 73.55% |
| 120 | 110.68% | 88.95% | 96.22% | 60.30% | 69.88% |
The MMV increasing value for the embodiment 1-3 for being added to tremella polysaccharides moisturizer known to the result of table 6 and Fig. 1 is obvious
Higher than un-added comparative example 1-2, and the MMV value highest of embodiment 1 and in 2h, the fluctuation of MMV value is not especially acutely, to say
Bright addition tremella polysaccharides moisturizer can obviously increase the moisture retention of moisture-retention gel water, and reach one strong in a longer period of time
Imitate the purpose of moisturizing.
6. percutaneous moisture loss TEWL test method
6.1 test philosophy
The measuring principle of the test apparatus derives from FICK Fick's law of diffusion dm/dt=D.A.dp/dx.Pass through two groups of temperature
The water vapor pressure ladder that degree, the nearly epidermis of humidity sensor measuring (about in lcm) are formed by cuticula moisture loss in different bright spots
Degree, directly measures the amount of moisture percutaneously distributed.TEWL value is an important symbol of skin barrier quality, the TEWL value of skin
It is lower, illustrate that the barrier function of skin is better, on the contrary it is poorer.
6.2 experimental conditions and volunteer require
Test environment conditions: test environment temperature is 22 ± 1 DEG C, and humidity is 50 ± 5%, and carries out dynamic in real time and examine
It surveys；
Volunteer requires: effective volunteer at least 30 people, the age, (gestation or breast feeding women removed between 16-65 years old
Outside)；Forearm test zone capacitance method moisture of skin measures the basic value of meaning between 15-100；Without serious systemic disease, nothing
Immune deficiency or autoimmune disease person；No active anaphylactic disease person；Previously to skin-care cosmetics without severe allergy history
Person；Hormone medicine and immunosuppressor person are not used in nearly one month；To participate in other clinical test persons；Make by regulation
With test medicine and all information；All volunteers should fill in informed consent form before testing.
Prepare before 6.3 tests
Any product (cosmetics or topical drug) cannot be used within 2-3 days before recipient site.Before experiment, subject needs same
It is surveyed in meaning cleaning both hands forearm, with dry face tissue wiped clean.It is surveyed in subject's both hands forearm after cleaning and carries out measured zone
Label.At least 30min that sits quietly in room should be being complied with standard before official testing, cannot drunk water, forearm exposure is in test mode
It places, keeps loosening.
6.4 testing procedure
3 × 3cm of label on the inside of left and right arm in experiment2Resistance to test area, same arm can mark multiple regions, region
It is spaced lcm.Test product and blank control are randomly dispersed in left and right arm.Using capacitance method skin analyzer carry out by
Try the measurement in region and control zone.Each region is according to parallel determination 15 times.The plain pape of each test zone is first measured, so
2.0 ± 0.1mg sample/cm is pressed afterwards2Dosage, test is spread evenly across in trial zone using latex finger cot.Smearing is surveyed respectively
The moisture content of skin (being measured when verifying by this time) in amount 30min, 60min, 120min, tested region and blank control region,
The test of the same volunteer is completed by the same survey crew.
6.5 test data
Measure the TEWL value of day part respectively by experiment.
Calculation formula are as follows: T%=(T1-T0)/T0× 100%
Note: T%=moisture of skin, which percutaneously scatters and disappears, reduces percentage (TEWL)；
T0=skin is percutaneously scattered and disappeared using moisture before sample to be worth；
T1=skin is percutaneously scattered and disappeared using moisture after sample to be worth；
The table 5 of the moisture-retention gel water embodiment 1-3 and comparative example 1-2 of 6.6 moisturizer containing tremella polysaccharides matched in such as 5.6
It is shown:
6.7 measure TEWL value result such as table 7:
The TEWL gain percentage of each period of table 7
| Time/min | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 |
| 0 | 0 | 0 | 0 | 0 | 0 |
| 30 | - 18.77% | - 9.60% | - 10.57% | - 7.21% | - 6.88% |
| 60 | - 11.20% | - 10.22% | - 13.01% | - 4.96% | - 4.95% |
| 120 | - 5.50% | 2.12% | - 6.50% | - 1.37% | 1.48% |
The TEWL increasing value for the embodiment 1-3 for being added to tremella polysaccharides moisturizer known to the result of table 7 and Fig. 2 is obvious
TEWL value lower than un-added comparative example 1-2, and the embodiment 1 embodiment 2-3 that compares is lower, and be maintained in 2h-
On 5.50%, illustrate that the function that moisture-retention gel water protects skin barrier can be enhanced by adding tremella polysaccharides moisturizer, further
Enhance the effect of moisturizing.
7. product effect assessment
Tremella polysaccharides moisture-retention gel water synergy moisturizing effect known to the experiment of above-mentioned moisture content of skin in embodiment 1
Fruit is best, carries out product effect assessment to it, sequentially includes the following steps:
(1) it invites 90 between 20~50 years old, the female volunteers of skin health, is randomly divided into 9 groups, every group of 20 people put down
Equal 28 ± 2 years old age tries out the tremella polysaccharides moisture-retention gel water in embodiment one respectively, and observes and experience using effect.
(2) application method: after volunteer cleans face, being uniformly applied to face for above-mentioned moisturizing cosmetic, uses 2 daily
It is secondary, adhere to using one month, at least checks that once whether there is or not whole bodies and local adverse reaction with understanding weekly, and record knot in detail
Fruit.Test assessment result such as the following table 8:
8 staining effect table of table
Experiment conclusion: the present invention is real to be liked and is approved by volunteer on probation deeply, and trying out volunteer indicates moisturizing cosmetic
The effect of moisturizing moisturizing is good, feel after trying out one month dry skin, the skin problems such as coarse, low and deep be improved significantly, skin
Skin water profit is smooth.
All there is allergic phenomena in non-someone in volunteer on probation simultaneously, does not also occur pruitus, shouting pain, redness etc.
Situation.Therefore, the tremella polysaccharides moisturizing cosmetic in the present invention is mildly safe, can be used for a long time, there is potent moisturizing.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (9)
1. a kind of moisturizer containing tremella polysaccharides, which is characterized in that be made by following components in parts by weight: tremella polysaccharides 20-40
Part, 10-20 parts of oligomerization hyaluronic acid, 5-10 parts of L- rhamnose, 3-5 parts of D-pantothenyl aleohol and 25-62 parts of deionized water；Each component weight
Part summation is 100 parts.
2. a kind of moisturizer containing tremella polysaccharides according to claim 1, which is characterized in that by following raw material system
: 25 parts of tremella polysaccharides, 12 parts of oligomerization hyaluronic acid, 8 parts of L- rhamnose, 3 parts of D-pantothenyl aleohol, 52 parts of deionized water.
3. a kind of moisturizer containing tremella polysaccharides according to claim 1, is made: tremella polysaccharides by following raw material
30 parts, 18 parts of oligomerization hyaluronic acid, 6 parts of L- rhamnose, 4 parts of D-pantothenyl aleohol, 42 parts of deionized water.
4. a kind of moisturizer containing tremella polysaccharides according to any one of claims 1 to 3, which is characterized in that the silver
Fungus polysaccharides are made by following steps: (1) weighing 10 parts by weight Tremella fructifications and mix with the deionized water of 500 parts by weight, be beaten
Afterwards with 200 mesh filter-cloth filterings, filtrate is collected, the dehydrated alcohol of 3 times of volumes of filtrate is added, for 24 hours, centrifuging and taking precipitating is dry for alcohol precipitation
Up to tremella Thick many candies；(2) refine: the tremella for taking the 10 parts by weight step (1) of deionized water dissolving of 100 parts by weight to prepare is slightly more
Sugar obtains tremella Thick many candies solution, is added and the isometric Sevage reagent of tremella Thick many candies solution, sufficiently 20 min of oscillation
Afterwards, it is centrifuged 15min under conditions of 5000r/min, collects upper layer polysaccharide solution, then addition is molten with polysaccharide into upper layer polysaccharide solution
The isometric Sevage reagent of liquid, repeats the above steps 9 times；(3) it dialyses: the polysaccharide solution after step (2) removing protein is put into
In the bag filter that 44mm molecular cut off is 3000,4 DEG C of deionised waters dialysis 48h, every 6h change a deionized water, are added saturating
The dehydrated alcohol of 3 times of volumes of polysaccharide solution of analysis precipitates, and filters to obtain precipitating, finally freeze-drying precipitates to obtain tremella polysaccharides.
5. a kind of moisturizer containing tremella polysaccharides according to claim 4, it is characterised in that: the mashing temperature of step (1)
It is 80 DEG C, beating time 7min.
6. a kind of preparation method of the moisturizer containing tremella polysaccharides, which comprises the following steps:
Under B1, room temperature, the tremella polysaccharides of 20-40 parts by weight and the oligomerization hyaluronic acid of 10-20 parts by weight are weighed, with 30-40 weight
The deionized water dissolving of part is measured, mixing makes it dissolve；
B2, the L- rhamnose of 5-10 parts by weight and the D-pantothenyl aleohol of 3-5 parts by weight are added by weight in step B1 solution,
It is added and is uniformly mixed while stirring under 120r/min；
B3, the deionized water that 60-70 parts by weight are added mix to get the moisturizer containing tremella polysaccharides.
7. a kind of cosmetics of preparation of moisturizer containing tremella polysaccharides according to claim 1-5, it is characterised in that:
The additive amount of the moisturizer containing tremella polysaccharides is 15-28 parts by weight.
8. a kind of cosmetics of preparation of moisturizer containing tremella polysaccharides according to claim 7, which is characterized in that by following heavy
Amount part component is made: 15-28 parts of moisturizer containing tremella polysaccharides, 940 0.5 parts of card wave, and 0.1-0.5 parts of arginine, preservative 0.1-
1 part, 70-85 parts of deionized water, each component parts by weight summation is 100 parts.
9. a kind of preparation method of the cosmetics of preparation of moisturizer containing tremella polysaccharides according to claim 7, feature exist
In, comprising the following steps:
Under S1, room temperature, in dust proof workshop, the tremella polysaccharides moisturizer of 15-28 parts by weight, the carbomer of 0.5 parts by weight are weighed
The arginine of 940,0.1-0.5 parts by weight, in reaction kettle, mixing makes it dissolve the preservative of 0.1-1 parts by weight, carries out ultraviolet
Line sterilization measure, referred to as A phase are spare；
S2, the deionized water that 70-85 parts by weight are added in uniform solution is mixed toward A, be uniformly mixed at 100r/min, into
Row ultraviolet disinfection disinfectant measure to get a kind of moisturizer containing tremella polysaccharides cosmetics.
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| CN114432166A (en) * | 2022-02-17 | 2022-05-06 | 广州欧正化妆品技术研究院有限公司 | Moisturizing mask liquid of silicon extract tremella polysaccharide |
| CN115137656A (en) * | 2022-08-25 | 2022-10-04 | 山东禾三千医疗科技有限公司 | Mild skin-brightening composition and application thereof |
| CN115487091A (en) * | 2021-06-18 | 2022-12-20 | 伽蓝（集团）股份有限公司 | Compound polysaccharide and preparation method and application thereof |
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