Specific embodiment
Below by drawings and examples, the present invention is described in more detail.Illustrated by these, the features of the present invention
It will be become more apparent from advantage clear.
In a first aspect, the present invention provides a kind of cistanche deserticola seed blanket, as shown in Figure 1, the seed blanket include by down toward
On the tilting blanket, attachment, cistanche deserticola seed and the covering that are sequentially distributed.
The present inventor can significantly improve meat the study found that the form that seed blanket is made in cistanche deserticola seed is sowed
The utilization efficiency of desert cistanche seed.
Wherein, seed blanket is a kind of processing method of seed pelleting, is to dissipate seed at tufted or strip or at random at random
Cloth is on paper or width made of other degradation materials and the seed dispersal unit on thin tilting blanket.
In further preferred embodiment, the tilting blanket is newspaper blanket, ox-hide blanket or nontoxic pulp based paper blanket.
In embodiment still more preferably, the size of the tilting blanket is (10~20) cm × (20~40) cm,
Such as 10cm × 20cm or 20cm × 40cm.
The distribution area of a kind of preferred embodiment according to the present invention, the attachment is identical as tilting blanket, described attached
Object include solid component and additive.
In further preferred embodiment, the solid component is selected from polyvinyl alcohol, polyvinyl acetate, Methyl cellulose
Two or more in element, carboxymethyl cellulose, hydroxypropyl cellulose, starch, Attapulgite Clay Minerals and admaic earth.
In embodiment still more preferably, the solid component is selected from polyvinyl alcohol, methylcellulose, hydroxypropyl
Two or more in cellulose, starch, Attapulgite Clay Minerals and admaic earth.
Preferably, the solid component is the mixture of Attapulgite Clay Minerals and admaic earth, the weight ratio of the two be (0.8~
1.2): 1, preferably 1:1.
The present inventor has unique rod-like fibre the study found that attapulgite is a kind of natural 1-dimention nano mineral
Structure is potential environment-friendly rubber supporting material, has water conservation adsorption function;Admaic earth is the mine of adsorptivity and good water-retaining property
Goods and materials source.Attapulgite and admaic earth are pressed (0.8~1.2): 1, preferably 1:1 are mixed, and are made into slimy attachment, can be protected
Hundred times of own wt of water is held and absorbed, provides adequate water for cistanche deserticola seed sprouting, solves to encounter when germination dry
Non-irrigated problem, while the strong rubber of attapulgite is seed sticking on tilting blanket and sticking for upper layer covering provides guarantor
Card makes seed blanket keep higher intensity, toughness and integrality.
A kind of preferred embodiment according to the present invention, the additive include water-retaining agent, growth-promoting root agent and the degeneration-resistant factor,
The mass ratio of three is 0.5:(0.003~0.009): (0.005~0.012), it is preferable that the mass ratio of three is 0.5:
(0.004~0.008): (0.006~0.01).
According to the present invention in a kind of preferred embodiment, the water-retaining agent is polyacrylamide, Sodium Polyacrylate or shallow lake
One of powder grafted propylene hydrochlorate is a variety of.
In further preferred embodiment, the water-retaining agent is starch grafted acrylate, such as starch-grafted propylene
Sour sodium.
Another preferred embodiment according to the present invention, the growth-promoting root agent are methyl α-naphthyl acetate, heteroauxin, indoles fourth
One of acid, naphthyloxyacetic acid and oxybenzene compound are a variety of.
In further preferred embodiment, the growth-promoting root agent is in methyl α-naphthyl acetate, heteroauxin and naphthyloxyacetic acid
It is one or more.
In embodiment still more preferably, the growth-promoting root agent is methyl α-naphthyl acetate.
Another preferred embodiment according to the present invention, the growth-promoting root agent include the component of following weight proportion:
In further preferred embodiment, the growth-promoting root agent includes the component of following weight proportion:
In embodiment still more preferably, the growth-promoting root agent includes the component of following weight proportion:
Wherein, methyl α-naphthyl acetate and brassin lactones have the function of increasing root system of plant, and diethyl aminoethyl hexanoate can be improved plant peroxide
The activity of compound enzyme and nitrate reductase promotes the division and elongation of plant cell, promotes the development of root system, adjusts in plant
The balance of nutrient, above three ingredient are mixed according to special ratios, can preferably promote the proliferation and growth of host's root system, from
And further increase the parasitic rate and germination rate of Herba Cistanches.
A kind of preferred embodiment according to the present invention, the compound bacteria is by bacillus laterosporus, bafillus natto, light
The bacterium powder for closing bacterium and actinomyces is combined.
In further preferred embodiment, the bacillus laterosporus, bafillus natto, photosynthetic bacteria and unwrapping wire
The weight ratio of bacterium is 1:(1.5~2.5): (0.5~1.5): (2~4), preferably 1:(1.8~2.2): (0.7~1.3): (2.5
~3.5), more preferably 1:2:1:3.
Wherein, in use, living bacteria count >=2 × 10 in additive8cfu/g。
The present inventor can remarkably promote the life of host's root system the study found that by adding compound bacteria in growth-promoting root agent
Long, so that the generation of lateral root adventitious root is more, root of hair is fast, and can promote and maintain root system activity, improves the degeneration-resistant border ability of host;
Meanwhile by adding compound bacteria, host's root system can be stimulated to secrete metabolite, to accelerate the sprouting and suction of cistanche deserticola seed
The formation of device improves germination rate and parasitic rate.
A kind of preferred embodiment according to the present invention, the degeneration-resistant factor are selected from gibberellin, pentaamino levulic acid, shell
One of glycan, salicylic acid and sodium nitroprusside are a variety of.
Wherein, the degeneration-resistant factor be mainly used for improving seed sprout to damage to plants caused by sudden drop in temperature, the tolerance of the adverse circumstances such as saline and alkaline, including plant
Object hormone or chemical substance with plant hormone characteristic.
In further preferred embodiment, the degeneration-resistant factor is selected from gibberellin, pentaamino levulic acid, chitosan
With one of salicylic acid or a variety of, preferably salicylic acid.
The present inventor the study found that salicylic acid can promote cistanche deserticola seed sprouting and improve Herba Cistanches resistance,
So as to significantly improve the germination rate of cistanche deserticola seed.
A kind of preferred embodiment according to the present invention, the additive further include auxiliary agent, the water conservation based on 0.5 parts by weight
Agent, the additive amount of the auxiliary agent are 0.002~0.005 parts by weight.
In further preferred embodiment, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
2~4 parts of Radix Sophorae Flavescentis extractive liquid
3~6 parts of Bai Cigen extracting solution.
In embodiment still more preferably, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
2.5~3.5 parts of Radix Sophorae Flavescentis extractive liquid
4~5 parts of Bai Cigen extracting solution.
Preferably, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
3 parts of Radix Sophorae Flavescentis extractive liquid
4 parts of Bai Cigen extracting solution.
The present inventor is the study found that in the planting process of Herba Cistanches, host, as haloxylon ammondendron be easy to happen root rot or
Corroded by pest (such as root mite), influences the parasitism of Herba Cistanches.And radix bupleuri extracting solution and Radix Sophorae Flavescentis extractive liquid can effectively inhibit meat desert
The pest and disease damage of Rong parasitic plant, especially Radix Sophorae Flavescentis extractive liquid, wherein a large amount of flavone compounds contained, can be effectively reduced plant
The incidence of insect disease of object prevents host's trunk or root system from being destroyed;Bai Cigen extracting solution can effectively improve Herba Cistanches kind
The germination rate of son.
Therefore, radix bupleuri extracting solution, Radix Sophorae Flavescentis extractive liquid and the Bai Ci of said ratio is added in the present invention preferably in additive
Root extracting solution significantly improves the germination rate and parasitic rate of cistanche deserticola seed.
A kind of preferred embodiment according to the present invention, the cistanche deserticola seed are by screening, clean and selected kind
Son.
Wherein, with the riddle of 1.2mm, the big impurity in cistanche deserticola seed is removed, it will be in seed with the sieve of 0.5mm
Sandy soil removal, then with compact spiral tower by seed other impurities removing, obtain full consistent high vigor seed.
A kind of preferred embodiment according to the present invention, the cistanche deserticola seed are evenly distributed on the upper surface of attachment,
The quantity for the cistanche deserticola seed for including on every piece of tilting blanket is 5~20, preferably 5~15.
In further preferred embodiment, the covering is toilet paper, and size is identical as the size of tilting blanket.
Second aspect, the present invention provides a kind of preparation process of cistanche deserticola seed blanket described in first aspect, the technique
The following steps are included:
Step 1, seed screening, clean and selected processing;
Step 2, attachment is prepared;
Step 3, seed blanket is prepared.
The preparation process of cistanche deserticola seed blanket of the present invention described further below:
Step 1, seed screening, clean and selected processing.
A kind of preferred embodiment according to the present invention, the seed screening includes successively being sieved with shaker screen and small sieve
Choosing,
Wherein, the aperture of the shaker screen be 0.6~1.4mm, preferably 0.8~1.2mm, such as 1.0mm, and/or
The aperture of the small sieve is 0.3~0.8mm, preferably 0.4~0.7mm, such as 0.5mm.
In further preferred embodiment, after screening, it is preferred to use compact spiral tower seed clean and
It is selected.
In the present invention, clean selected by above-mentioned, selection obtains full, neat, healthy and strong cistanche deserticola seed.
Step 2, attachment is prepared.
A kind of preferred embodiment according to the present invention, the attachment include solid component and additive, and the two is by pre-
Certainty ratio is mixed to form slimy attachment.
In further preferred embodiment, the solid component is selected from polyvinyl alcohol, polyvinyl acetate, Methyl cellulose
Two or more in element, carboxymethyl cellulose, hydroxypropyl cellulose, starch, Attapulgite Clay Minerals and admaic earth.
In embodiment still more preferably, the solid component is selected from polyvinyl alcohol, methylcellulose, hydroxypropyl
Two or more in cellulose, starch, Attapulgite Clay Minerals and admaic earth.
Preferably, the solid component is the mixture of Attapulgite Clay Minerals and admaic earth, the weight ratio of the two be (0.8~
1.2): 1, preferably 1:1.
A kind of preferred embodiment according to the present invention crush to solid component and sieving is handled,
Wherein, the sieving was 50~110 meshes, preferably 60~100 meshes.
A kind of preferred embodiment according to the present invention, the additive include water-retaining agent, growth-promoting root agent and the degeneration-resistant factor,
The mass ratio of three is 0.5:(0.003~0.009): (0.005~0.012), it is preferable that the mass ratio of three is 0.5:
(0.004~0.008): (0.006~0.01).
According to the present invention in a kind of preferred embodiment, the water-retaining agent is polyacrylamide, Sodium Polyacrylate or shallow lake
One of powder grafted propylene hydrochlorate is a variety of.
In further preferred embodiment, the water-retaining agent is starch grafted acrylate, such as starch-grafted propylene
Sour sodium.
Another preferred embodiment according to the present invention, the growth-promoting root agent are methyl α-naphthyl acetate, heteroauxin, indoles fourth
One of acid, naphthyloxyacetic acid and oxybenzene compound are a variety of.
In further preferred embodiment, the growth-promoting root agent is in methyl α-naphthyl acetate, heteroauxin and naphthyloxyacetic acid
It is one or more.
In embodiment still more preferably, the growth-promoting root agent is methyl α-naphthyl acetate.
Another preferred embodiment according to the present invention, the growth-promoting root agent include the component of following weight proportion:
In further preferred embodiment, the growth-promoting root agent includes the component of following weight proportion:
In embodiment still more preferably, the growth-promoting root agent includes the component of following weight proportion:
A kind of preferred embodiment according to the present invention, the compound bacteria is by bacillus laterosporus, bafillus natto, light
The bacterium powder for closing bacterium and actinomyces is combined.
In further preferred embodiment, the bacillus laterosporus, bafillus natto, photosynthetic bacteria and unwrapping wire
The weight ratio of bacterium is 1:(1.5~2.5): (0.5~1.5): (2~4), preferably 1:(1.8~2.2): (0.7~1.3): (2.5
~3.5), more preferably 1:2:1:3.
A kind of preferred embodiment according to the present invention, the degeneration-resistant factor are selected from gibberellin, pentaamino levulic acid, shell
One of glycan, salicylic acid and sodium nitroprusside are a variety of.
Wherein, the degeneration-resistant factor be mainly used for improving seed sprout to damage to plants caused by sudden drop in temperature, the tolerance of the adverse circumstances such as saline and alkaline, including plant
Object hormone or chemical substance with plant hormone characteristic.
In further preferred embodiment, the degeneration-resistant factor is selected from gibberellin, pentaamino levulic acid, chitosan
With one of salicylic acid or a variety of, preferably salicylic acid.
The present inventor the study found that salicylic acid can promote cistanche deserticola seed sprouting and improve Herba Cistanches resistance,
So as to significantly improve the germination rate of cistanche deserticola seed.
A kind of preferred embodiment according to the present invention, the additive further include auxiliary agent, the water conservation based on 0.5 parts by weight
Agent, the additive amount of the auxiliary agent are 0.002~0.005 parts by weight.
In further preferred embodiment, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
2~4 parts of Radix Sophorae Flavescentis extractive liquid
3~6 parts of Bai Cigen extracting solution.
In embodiment still more preferably, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
2.5~3.5 parts of Radix Sophorae Flavescentis extractive liquid
4~5 parts of Bai Cigen extracting solution.
Preferably, the auxiliary agent includes the component of following weight proportion:
5 parts of radix bupleuri extracting solution
3 parts of Radix Sophorae Flavescentis extractive liquid
4 parts of Bai Cigen extracting solution.
A kind of preferred embodiment according to the present invention, the radix bupleuri extracting solution, Radix Sophorae Flavescentis extractive liquid and Bai Cigen extracting solution
Extracted by the step of crushing, immersion, microwave treatment.
In further preferred embodiment, radix bupleuri, kuh-seng and Bai Cigen are crushed to 20~40 mesh, preferably 25
~35 mesh.
In embodiment still more preferably, smashed radix bupleuri, kuh-seng and Bai Cigen are separately added into accordingly
Solution impregnates, wherein
The radix bupleuri is added water saturated n-butanol and impregnates, and solid-to-liquid ratio is 1:(8~12), preferably 1:10;
The acetone soln that concentration is 8%~12% is added in the kuh-seng to impregnate, solid-to-liquid ratio is 1:(12~18), preferably
1:(13~16), such as 1:15;
The Bai Cigen is added water and impregnates, and solid-to-liquid ratio is 1:(3~6), preferably 1:(4~6), such as 1:5.
A kind of preferred embodiment according to the present invention, the microwave treatment time of the radix bupleuri extracting solution is 4~6min, micro-
Wave power is 500~700W;
The microwave treatment time of the Radix Sophorae Flavescentis extractive liquid is 8~12min, and microwave power is 550~800W;
The microwave treatment time of the Bai Cigen extracting solution is 10~15min, and microwave power is 400~600W.
In further preferred embodiment, the microwave treatment time of the radix bupleuri extracting solution is 5min, microwave power
For 550~650W, such as 600W;
The microwave treatment time of the Radix Sophorae Flavescentis extractive liquid is 9~11min, and microwave power is 600~700W, such as microwave treatment
Time is 10min, microwave power 650W;
The microwave treatment time of the Bai Cigen extracting solution is 12~14min, and microwave power is 450~550W, such as microwave
The processing time is 13min, microwave power 500W.
The present inventor can be improved extracting efficiency the study found that using microwave-assisted extraction, improve active principle content,
To improve the parasitic rate of Herba Cistanches.
Solid component and additive according to weight ratio are (60~100) by a kind of preferred embodiment according to the present invention:
20 are mixed, and slimy attachment is prepared.
In further preferred embodiment, the weight ratio of the solid component and additive is (70~90): 20, it is excellent
It is selected as 80:20.
Wherein, the additive applies as a solution, water-retaining agent in preferable additives solution, growth-promoting root agent and degeneration-resistant
The mass concentration of the factor is respectively 0.5%, 0.003%~0.009%, 0.005%~0.012%.
Step 3, seed blanket is prepared.
Wherein, step 3 includes following sub-step:
Step 3-1, prepares tilting blanket and coating.
A kind of preferred embodiment according to the present invention, the tilting blanket be newspaper blanket, ox-hide blanket or nontoxic pulp based paper blanket,
The size of the tilting blanket is (10~20) cm × (20~40) cm, such as 10cm × 20cm or 20cm × 40cm.
In further preferred embodiment, the covering is toilet paper, and size is identical as the size of tilting blanket.
The attachment prepared in step 2 is uniformly applied to tilting blanket surface, then sowed seeds on attachment by step 3-2.
Wherein, the muddy attachment prepared in step 2 is uniformly applied to the surface of tilting blanket, then by 5~20
Cistanche deserticola seed uniform broadcasting that treated in step 1 is in the upper surface of attachment.
Covering is covered on the upper surface of seed by step 3-3, dry, and the cistanche deserticola seed blanket is made.
Wherein, the covering is preferably toilet paper, is covered to the upper surface of cistanche deserticola seed, flattens, then exist
It dries in the shade under the conditions of 20~30 DEG C, is packed after the assay was approved.
The third aspect, the present invention provides cistanche deserticola seed blankets made from a kind of preparation process according to second aspect.
Fourth aspect, the present invention provides a kind of implantation methods of Herba Cistanches, it is preferred to use meat desert described in first aspect
Rong's seed blanket is inoculated with, the implantation methods the following steps are included:
Step I, selection of land plant host.
In 4~May, host is planted in the selection sandy loam that topography is flat, permeability is strong or aeolian sandy soil area, it is preferred to use opens
Ditch drilling method plants host.
A kind of preferred embodiment according to the present invention, the host are haloxylon ammondendron, red building or Chinese tamarisk, preferably haloxylon ammondendron
And/or Chinese tamarisk.
In further preferred embodiment, the spacing of the host of the plantation is no more than 1.5m, and line-spacing is no more than 4m.
In embodiment still more preferably, the planting density of the host is 100~400 plants/acre, preferably
150~350 plants/acre, more preferably 250 plants/acre.
A kind of preferred embodiment according to the present invention first carries out fertilizer treatment, institute to planting area before planting host
The fertilizer for stating application includes the component of following weight proportion:
15 parts of urea
2~6 parts of phosphorus diamines
5~15 parts of fulvic acid concentrate.
Preferably, the fertilizer for stating application includes the component of following weight proportion:
15 parts of urea
3~5 parts of phosphorus diamines
6~13 parts of fulvic acid concentrate.
The inventors discovered that fulvic acid is due to having stronger complexing, chelating and table containing functional groups such as hydroxyl, phenolic hydroxyl groups
Face adsorption capacity can be reduced the loss of ammonium nitrogen;Phosphorus moving distance in the soil can be increased, inhibit soil to water-soluble phosphorus
It is fixed, invalidate phosphorus and be converted into available phosphorus, promotes absorption of the root system to phosphorus;Fulvic acid can also absorb storage potassium ion, improve
The content of effective potassium is obvious to potash fertilizer synergy.
In the present invention, apply the viability that above-mentioned fertilizer is conducive to improve host, promote the fast-growth of host's root system,
To improve the rate of vaccination of Herba Cistanches.
In further preferred embodiment, the applied amount of the fertilizer is 7~13kg/ mus, preferably 10kg/ mus.
Step II, inoculating cistanche salsa.
A kind of preferred embodiment according to the present invention is laid with cistanche deserticola seed blanket after host plants 2 years or 3 years
It is inoculated with, the inoculation time is spring or autumn.
In further preferred embodiment, plantation hole is dug at 40~60cm of two sides of host's base portion, is preferably being posted
At main 42~48cm of base portion two sides, as dug plantation hole at 45cm.
The present inventor is the study found that at host's base portion 40~60cm of two sides, at well developed root system at preferably 42~48cm, in
It is laid with seed blanket herein, is conducive to the parasitic rate for improving Herba Cistanches.
In embodiment still more preferably, the diameter of the plantation hole is 40~50cm, and depth is 70~80cm,
Preferably, the diameter of the plantation hole is 45cm, depth 60cm.
Wherein, the root system preferably dug to host exposes.
A kind of preferred embodiment according to the present invention is laid with seed blanket, so that the coating of seed blanket in plantation hole
Side is close to host's root system.
Wherein, full hole is backfilled after being laid with seed blanket, is then watered.
Step III, field management.
In the present invention, the annual season of growth after inoculation, host is fostered, guarantees host's robust growth.One
As every year according to sand ground soil moisture content, drip 2-3 times, using physics and chemistry combine by the way of, prevent and treat sacsaoul pest and disease damage.