CN110200005A - Microbicide compositions and its controlled release system and application - Google Patents
Microbicide compositions and its controlled release system and application Download PDFInfo
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- CN110200005A CN110200005A CN201910602409.3A CN201910602409A CN110200005A CN 110200005 A CN110200005 A CN 110200005A CN 201910602409 A CN201910602409 A CN 201910602409A CN 110200005 A CN110200005 A CN 110200005A
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- controlled release
- microbicide compositions
- release system
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- aldehyde
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/46—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=C=S groups
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Abstract
The present invention provides a kind of microbicide compositions and its controlled release system and application.The effective component of the microbicide compositions and its controlled release system is allyl isothiocyanate and aldehyde compound.The control efficiency of microbicide compositions and controlled release system Phytophthora infestans provided by the invention is good, can long-acting control fungus damage, reduce times for spraying, reduce drug cost.Allyl isothiocyanate is used alone or can effectively control phytophthora infestans with the combination of certain aldehyde, and the lasting period is up to 3 months.Also, some combinations of allyl isothiocyanate and aldehyde have synergistic function for prevention and treatment phytophthora infestans.
Description
Technical field
The invention belongs to biological pesticide technical fields, specifically, being related to a kind of microbicide compositions and its controlled release system
With application.
Background technique
Potato is always that the important food source of China may become more important as population increases.Ma Ling
Potato plant growth is rapid, and every per mu yield is high, can be in unsuitable cereal crops such as rice, the grown on soil of wheat and corn.
It is often stored in soil for edible potato and digs out when needed, therefore the aboveground storage time is short, wherein about
10% crop must save for (in the future) plantation.
Influence of the plant development of potato vulnerable to certain pest and disease damages, these pest and disease damages limit the production of potato.Evening
Epidemic disease bacterium (Phytophthora infestans) is a kind of oomycete pathogen, leaf, stem, fruit and the block of the potato that can cause harm
Stem.It is the potato disease of most serious in the world, leads to 1845 to 1849 Ireland outburst potato famines.
When late disease bacteria grows and breeds rapidly on host crop, it may occur that serious epiphytolosy.It passes through
The sporangium that infected plant tissue generates is bred, and under conditions of high-moisture and moderate temperature (15-25 DEG C)
Breeding it is most quick.Sporangium is flowed by the air in rainwater splashing or the flowing of field air and warehouse expands late disease bacteria
It is scattered in health tissues.Each sporangium can cause new lesion.In general, the temperature in potatoes storage facilities
The growth and propagation of late disease bacteria are very suitable to humidity.Therefore, the late disease bacteria of the seed potato in control storage is control
Make the pith of this serious crop disease.
Potato must be stored in it is mild under conditions of, including good air circulation and mild temperature condition, these
It can prevent various storage diseases.The air flowing harvested in the potato caused harm and potato storage can all promote late blight
The propagation of germ.Although being visible and can be removed in the potato of Storage period early infection, in secondary cycles
Symptom may not occur in middle infection.During plantation, contact seed potato can lead to stem tuber of the disease from disease infection
It is propagated to the stem tuber of health.
Wood etc. (2017) is although thinking that E-2- hexenoic aldehyde is stifling in research before is certain that can control potato
A little diseases, including potato anthracnose (Colletotrichum coccodes), potato silver maize ear rot
(Helminthosporium solani) and soft rot (Pectobacterium atrosepticum), but without any report
Show that the compound can control late disease bacteria.Also, controlled release system is not used when the compound discharges, therefore inevitably
Lasting period is shorter.
Therefore, the method for the new achievable continuous and effective prevention and treatment phytophthora infestans of exploitation is needed.
Summary of the invention
The object of the present invention is to provide a kind of microbicide compositions and its controlled release system and applications.
The study found that allyl isothiocyanate and valeral, hexanal, enanthaldehyde, octanal, E-2- hexenoic aldehyde, Z-3- hexenoic aldehyde, benzene
Formaldehyde, anisaldehyde and salicylaldehyde are effective fumigant of phytophthora infestans at low concentrations., it is surprising that different
Some combinations of allyl sulfocyanate and aldehyde have synergistic function for prevention and treatment phytophthora infestans.It finds accordingly, structure
Controlled release durg delivery system is built, and tests a series of polymer, develops the capsule and capillary controlled-release device of sealing.In addition, also
The sealing pouch for these compounds, particle, candle wick bottle and atomizer are had developed as controlled-release device.
In order to achieve the object of the present invention, in a first aspect, the present invention provide it is a kind of for preventing and treating the system of phytophthora infestans
Agent (fumigant), effective component are allyl isothiocyanate.
Second aspect, the present invention provide a kind of microbicide compositions, and effective component is allyl isothiocyanate and aldehydes
Close object.
Preferably, the aldehyde compound is aliphatic or aromatic aldehyde；More preferable valeral, hexanal, enanthaldehyde, octanal, nonyl
At least one of aldehyde, E-2- hexenoic aldehyde, Z-3- hexenoic aldehyde, benzaldehyde, anisaldehyde, salicylaldehyde, most preferably octanal, benzene first
Aldehyde.
In microbicide compositions of the invention, the weight ratio of allyl isothiocyanate and aldehyde compound is 1-99: 1-99.
Further, the weight ratio of allyl isothiocyanate and octanal is 1: 1.
The weight ratio of allyl isothiocyanate and benzaldehyde is 1: 1.
The third aspect, the present invention provide the controlled release system of the preparation or microbicide compositions, and the controlled release system is such as
Under 1.~any of 4.:
1. controlled release capsule: the seal capsule containing the preparation or microbicide compositions；
2. sustained-release granular formulation；It is adsorbed with the particulate inert carrier of the preparation or microbicide compositions；
3. candle wick bottle, vapourizing unit (such as sprayer, atomizer) or pipe (capillary), hermetic bag；
4. the preparation or microbicide compositions and the supplementary product compatibility that can reduce its effective component rate of release are resulting
Sustained release agent.
Wherein, the material of the capsule is permeable thermoplastic polymer, is selected from high density polyethylene (HDPE), low density polyethylene
Alkene, linear low density polyethylene, ethylene vinyl acetate, polyvinyl chloride, thermoplastic polyurethane, polyethers/amide block copolymers
At least one of, preferred polyvinyl chloride.
The particulate inert carrier is mineral or plant base carrier, is selected from kaolin, bentonite, atlapulgite, Hai Pao
Stone, White Carbon black, diatomite, precipitated calcium carbonate, talcum powder, the plaster of paris, chalky soil, clay, montmorillonite, clay, high-purity soil, wood
At least one of powder, bamboo powder, corn ear, preferably vermiculite.
In the present invention, the partial size of the particulate inert carrier is 0.1-10.0mm, preferably 2.1-3.0mm.
Further, the surface of the sustained-release granular formulation is equipped with coating layer, and coated fertilizer is selected from polyethylene oxide, poly- second two
The polymer of at least one of alcohol, stearic acid, paraffin, rosin or other releasable effective components.The coating material
Expect preferred polyethylene oxide.
Wherein, the weight ratio of the microbicide compositions, particulate inert carrier and coated fertilizer is 1-50: 50-90: 5-
10, preferably 35: 57.5: 7.5.
In the specific embodiment of the present invention, the sustained-release granular formulation is by 55% vermiculite, 35% isothiocyanic acid
Allyl ester and 10% polyethylene oxide (MW=5,000,000) composition.
The specific production method of the sustained-release granular formulation are as follows: allyl isothiocyanate and vermiculite are mixed, work is stirred evenly
For core material, after polyethylene oxide heating melting, even application in core surfaces to obtain the final product.
Fourth aspect, the present invention provide the preparation, microbicide compositions or the controlled release system in prevention and treatment phytophthora
Application in pathogen (especially phytophthora infestans) and its caused plant disease.
It is specific to fumigate in application, the dosage of the allyl isothiocyanate is 2.4g/m3More than, the dosage of aldehyde compound
For 2.4g/m3More than.
When the effective component of the microbicide compositions or controlled release system is allyl isothiocyanate and octanal, different sulphur cyanogen
The dosage of allyl propionate is 2.4-4.8g/m3, the dosage of octanal is 2.4-9.6g/m3.The two preferable amount ratio is 1-99: 99-1,
Preferably 1: 9,1: 1,9: 1, most preferably 1: 1 (mass ratio).
When the effective component of the microbicide compositions or controlled release system is allyl isothiocyanate and benzaldehyde, different sulphur
The dosage of cyanic acid allyl ester is 2.4-4.8g/m3, the dosage of benzaldehyde is 2.4-19.2g/m3.The two preferable amount ratio is 1-99:
99-1, preferably 1: 9,1: 1,9: 1, most preferably 1: 1 (mass ratio).
Preparation, microbicide compositions and controlled release system provided by the present invention for preventing and treating phytophthora infestans are suitable for
Limited space or interior use, stifling condition in open space are as follows: 5-30 DEG C of temperature.
By above-mentioned technical proposal, the present invention at least have following advantages and the utility model has the advantages that
The control efficiency of microbicide compositions and controlled release system Phytophthora infestans provided by the invention is good, can be long-acting
The disease is controlled, times for spraying is reduced, reduces drug cost.Allyl isothiocyanate or the combination energy with certain aldehyde is used alone
Enough effectively control phytophthora infestans, and the lasting period is up to 3 months.Also, some combinations of allyl isothiocyanate and aldehyde
There is synergistic function for prevention and treatment phytophthora infestans.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Late disease bacteria involved in embodiment 1-3 and 5 (phytophthora infestans), the public can obtain from China Agricultural University
?.Bibliography: Daayf F, Adam L and Fernando WGD (2003) Comparative screening of
bacteria for biological control of potato late blight(strainUS-8)using in
Vitro, detached leaves, and whole plant testing systems.Canadian Journal of
Plant Pathology, 25:276-284.
The percentage sign " % " being related in the present invention refers to mass percent if not specified；But the percentage of solution
Than unless otherwise specified, referring to the grams closed in 100mL solution and have solute.
Embodiment 1 simulates controlled release glue under storage environment and assists the test to late disease bacteria control effect
The present invention has studied the fumigant that may be had an impact to the late disease bacteria on the potato of storage.In order to determine
The actual efficacy for developing preparation, studies the potato of storage under room temperature (about 20 DEG C).Potato cleaning is clean
Afterwards, it places it on paper and is dried overnight, and put the potato of 10 kilograms of wash cleans in each carton, altogether 32 cartons.
The potato of these quantity can probably put half box.
Box by a controlled release capsule (including 1 gram of fumigant, ingredient is shown in Tables 1 and 2) with tape-stripping to each processing
Lid inside, five repetitions of each processing.Capsule is not placed in seven box covers for being used as control (experimental group).
Polymer for making capsule is high density polyethylene (HDPE), low density polyethylene (LDPE), linear low density polyethylene, ethylene
Vinyl acetate and their combination and polyvinyl chloride, thermoplastic polyurethane and polyethers/amide block copolymers
(PEBAX).4 millimeters of internal diameters, 0.5 millimeter of wall thickness and 100 millimeters long of pipe fitting are packed into 1 milliliter of allyl isothiocyanate and heat
Envelope.It under the conditions of ready-made capsule is placed in 21 DEG C~25 DEG C, and is weighed weekly, is measured 3 months or up to capsule with assay balance
It is emptying.Experimental result is shown in Table 1.For being suitable for those of allyl isothiocyanate polymer, be equally packed into capsule valeral,
Octanal and benzaldehyde are tested, and the results are shown in Table 2.
Rate of release of the table 1 from the variety classes polymer capsule of sealing release allyl isothiocyanate
As can be seen that PVC, LDPE and LDPE/EVA are the most suitable polymer for this controlled release capsule, wherein needing
It to use multiple Caplets (such as every bag one or two).When large-scale ventilation equipment are used for the gas stream of potato storage facility
When dynamic, EVA, TPU and PEBAX will be useful.
A series of rate of release of aldehyde of 2 sealed polymer capsule of table
As can be seen that the best polymer of controlled release capsule is PVC, it is most like with the rate of release of allyl isothiocyanate
Aldehyde is octanal.The combination, which has, discharges the effectiveness of substantially similar ratio in the their entire life of capsule control release device.
Seed potato is subjected to surface sterilization to it with 70% ethyl alcohol and is cut in half.It coats on the potato surface of cutting
One drop phytophthora infestans culture, the potato after inoculation is placed in sterile glass vials and is placed three weeks at room temperature.
With the white hypha growing state on micro- sem observation potato and confirm the susceptible situation of late disease bacteria.
By the potato quadrisection of inoculation, the middle position on the surface of potato is positioned to one in each box
Straight line.At the same time, retain the potato that two boxes only put purchase, as secondary " compareing without inoculum ", to reflect evening
The foundation level situation of epidemic disease bacterium.Box is sealed 30 days, box is then opened, checks whether potato infects late blight.It is logical
Microexamination is crossed with those of obvious late blight disease symptoms potato, observes whether it has the spore from late disease bacteria to deposit
?.It the results are shown in Table 3.
Table 3 uses allyl isothiocyanate and aldehyde Phytophthora infestans under the conditions of simulating storage of controlled release capsule
Control effect test
| It handles (milligram/controlled release capsule) | Infect the average percent of late disease bacteria |
| Control-non-treated-does not have inoculum | 2.0% |
| Control is-non-treated-inoculum | 39.2% |
| Allyl isothiocyanate (1000 milligrams) | 0.2% |
| Allyl isothiocyanate: octanal (500: 500 milligrams) | 0.5% |
| Allyl isothiocyanate: benzaldehyde (500: 500 milligrams) | 1.0% |
| Benzaldehyde (1000 milligrams) | 11.3% |
| Octanal (1000 milligrams) | 10.8% |
It can be seen that allyl isothiocyanate, allyl isothiocyanate-octanal and allyl isothiocyanate-benzaldehyde pair
It is highly effective to control phytophthora infestans, octanal is used alone and benzaldehyde effect is poor.
Embodiment 2 simulates test of the controlled release granule to late disease bacteria control effect under storage environment
It is granular preparation by another method that the desired amount of fumigant is transmitted to the potato in storage.Suitable controlled release
Particle can be dispersed on the potato bag of stacking, and particle can be fallen in sack, some can fall on the lower part of stacking, and fumigant steams
Gas will transmit through potato heap and be diffused into air.This release system can answer in the warehouse for having stacked potato bag
With, but have the shortcomings that the lasting period is short, it needs to reuse.For this purpose, with polyethylene oxide coating (PEO, MW 5,000,
000) mineral carrier (vermiculite) particle is tested.Polyethylene oxide coating accounts for the 10% of particle weight.Effective component is added in proportion
Enter in mineral base carrier particle.By BHT antioxidant in 2% concentration incorporation effective component.Sustained-release granular formulation (the controlled release
Particle) specific production method are as follows: by effective component, antioxidant and vermiculite mix, stir evenly as core material, by polycyclic oxygen
After ethane heating melting, even application in core surfaces to obtain the final product.
It is grouped according to effective component, it is specific as follows: allyl isothiocyanate, allyl isothiocyanate-octanal, different sulphur
Cyanic acid allyl ester-benzaldehyde, octanal, benzaldehyde.
(about 20 DEG C) test the potato in storage at room temperature.By potato cleaning it is clean after, make its
It is dried overnight on paper, 12 every casees of carton are put into 10 kilograms of potatos.These potatos can probably put half box.0.5 gram of control
It releases particle, loads 1 gram of fumigant (ingredient is shown in Table 4).Controlled release granule is not placed in five boxes for being used as control.
It will be inoculated with the potato quadrisection of phytophthora infestans, in each box the centre on the surface of potato
Place in alignment in position.At the same time, retain the potato that two boxes only put purchase, as secondary " without inoculum
Control ", the foundation level situation of Lai Fanying late disease bacteria.Box is sealed 30 days, box is then opened, whether checks potato
Infect late blight.There is those of obvious late blight disease symptoms potato by microexamination, observe whether it has from late blight
The spore of germ exists.It the results are shown in Table 4.
Table 4 uses allyl isothiocyanate and aldehyde Phytophthora infestans under the conditions of simulating storage of controlled release granule
Control effect test
| It handles (milligram/controlled release capsule) | Infect the average percent of late disease bacteria |
| Control-non-treated-does not have inoculum | 2.1% |
| Control is-non-treated-inoculum | 42.0% |
| Allyl isothiocyanate (88 milligrams) | 0.3% |
| Allyl isothiocyanate: octanal (44: 44 milligrams) | 0.4% |
| Allyl isothiocyanate: benzaldehyde (44: 44 milligrams) | 1.3% |
| Benzaldehyde (88 milligrams) | 16.0% |
| Octanal (88 milligrams) | 9.0% |
As can be seen that allyl isothiocyanate, allyl isothiocyanate-are pungent under conditions of using controlled release granule as carrier
Aldehyde and allyl isothiocyanate-benzaldehyde are highly effective to control phytophthora infestans.
Embodiment 3, which is simulated, uses test of the candle wick bottle controlled-release device to late disease bacteria control effect under storage environment
Fumigant is transported in the air stream by potato by candle wick bottle using the device to test for being similar to embodiment 2
Experiment.Herein without using particle as controlled release system.Respectively in two 5 liters of bucket, pass through one with 2 liters/min of rate
The pvc pipe pumped air of a 6 millimeters of internal diameters.20 milliliters of stifling agent solutions and 10 millimeters of aerial candles of cotton core exposure will be contained
Core bottle is put into the bottom of a bucket.Another bucket is not provided with candle wick bottle, as control.It is stayed after each barrel of sealing there are four outlet,
Its form is that 4mm internal diameter tube is inserted into the hole of each box, and carton uses rubber belt sealing.Net flow is 0.5 liter per minute stifling
Agent gas.
Potato is inoculated with according to the method provided in embodiment 1.By the potato quadrisection of inoculation, in each box
Place in alignment in the middle position on the surface of middle potato.At the same time, retain the potato that two boxes only put purchase,
As secondary " being compareed without inoculum ", the foundation level situation of Lai Fanying late disease bacteria.Box is sealed, candle wick bottle stifling 30
It, then opens box, checks whether potato infects late blight.There is that of obvious late blight disease symptoms by microexamination
Whether a little potatos observe it with the presence of the spore from late disease bacteria.It the results are shown in Table 5.
The control effect test of the air stream of 5 compound of table and mixture Phytophthora infestans in simulation storage
| Processing | Infect the average percent of late disease bacteria |
| Control-non-treated-does not have inoculum | 1.4% |
| Control is-non-treated-inoculum | 55.8% |
| Allyl isothiocyanate 100% | 0.8% |
| Allyl isothiocyanate: octanal 50: 50, mass ratio | 1.4% |
| Allyl isothiocyanate: benzaldehyde 50: 50, mass ratio | 1.1% |
As can be seen that all processing groups are all effective to late disease bacteria under the experiment condition.
Study of Sensitivity of the different late disease bacteria biological strains of embodiment 4 to AITC
Experiment purpose: a series of late disease bacteria Physiological Differentiation microspecies are tested to the sensibility of AITC.
Experimental method: by separated in potato 8 kinds of late disease bacteria Physiological Differentiation microspecies, 2 kinds of bacterium separated from tomato
Strain is inoculated on the culture medium of rye agar.On three culture dishes (diameter 6cm) equipped with rye agar, connect in center
The mycelia that the slave bacterial strain edge of kind 3 × 3mm of size takes.Culture dish is placed on the polypropylene box with 70% ethanol disinfection in advance
The bottom of (50 × 35 × 25cm).One controlled-release device containing 0.5g AITC is sticked to the bottom of box, as processing group.
Control group is arranged in box identical with processing group, connects bacterium according to the identical method of processing group, but do not contain in controlled-release device
AITC.The box of processing group and control group is placed in the 10 day time at room temperature, and whether observation judges to have in culture dish late later
Epidemic disease bacterium growth.Each processing is repeated 3 times (R1, R2, R3).It the results are shown in Table 6.
Sensitivity analysis of the different late disease bacteria biological strains of table 6 to AITC
Note: bacterium colony growing state is as follows :-: do not grow ,+: diameter 1-2cm, ++: diameter 2-3cm, +++: diameter >
3cm。
Experimental result: in processing group, observe that late disease bacteria is not grown on the culture dish for be vaccinated with late disease bacteria.It is right
According in group, there is the growth of mycelia after all culture dish inoculation late disease bacterias, but there is no mycelia raw in the chest of processing group
It is long.
Synergistic function of the composition for the aldehydes that 5 AITC of embodiment and spy walk to prevention and treatment late disease bacteria
Experiment purpose: the synergistic effect of verifying AITC and several aldehydes of selection in terms of inhibiting late disease bacteria.
Experimental method: the AITC of low dosage and the mixture of two kinds of aldehydes (hexanal and benzaldehyde) and these substances are tested
To the inhibiting effect of late disease bacteria, synergistic function of the mixture in terms of inhibiting late disease bacteria is determined.Rye fine jade is being housed
On three culture dishes (diameter 6cm) of rouge, in, heart position is inoculated with the late disease bacteria strains A that the slave bacterial strain edge of 3 × 3mm takes.
These are placed on the bottom of sterile 1.2 liters of vials.The candidate in 10 microlitres of ethyl alcohol will be dissolved in and fumigate compound drop viscous
On aseptic filter paper on bottle cap.The dosage of every 1.2 liters of vials is 0.25 microlitre, which is considered being low enough to prove association
Same-action.Bacterium tank is placed under room temperature (about 20 DEG C) and is saved 10 days, the growing state of bacterium is detected.It the results are shown in Table 7.
The synergistic function of table 7 AITC and aldehyde compound
Note: the % occurred in table is the white score of quality.
Experimental result: as can be seen from Table 7, allyl isothiocyanate, hexanal, benzaldehyde and their mixture are used
Phytophthora infestans all has inhibitory effect, but three of the above compound is used alone, the inhibition of late disease bacteria is imitated
Fruit, not as good as the inhibitory effect of allyl isothiocyanate and the mixture of hexanal and benzaldehyde, when allyl isothiocyanate and aldehydes
It is best for the inhibitory effect of late disease bacteria when the ratio of compound is 1: 1.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be modified or is improved, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. microbicide compositions, which is characterized in that effective component is allyl isothiocyanate and aldehyde compound；
Preferably, the aldehyde compound is aliphatic or aromatic aldehyde；More preferable valeral, hexanal, enanthaldehyde, octanal, aldehyde C-9, E-
At least one of 2- hexenoic aldehyde, Z-3- hexenoic aldehyde, benzaldehyde, anisaldehyde, salicylaldehyde, most preferably octanal, benzaldehyde.
2. composition according to claim 1, which is characterized in that the weight ratio of allyl isothiocyanate and aldehyde compound
It is 1-99: 1-99.
3. composition according to claim 2, which is characterized in that the weight ratio of allyl isothiocyanate and octanal is 1: 1；
And/or
The weight ratio of allyl isothiocyanate and benzaldehyde is 1: 1.
4. the controlled release system of any one of the claim 1-3 microbicide compositions, which is characterized in that the controlled release system is such as
Under 1.~any of 4.:
1. controlled release capsule: the seal capsule containing the microbicide compositions；
2. sustained-release granular formulation；It is adsorbed with the particulate inert carrier of the microbicide compositions；
3. candle wick bottle, vapourizing unit or pipe, hermetic bag；
4. by the microbicide compositions and the resulting sustained release agent of supplementary product compatibility that its effective component rate of release can be reduced.
5. controlled release system according to claim 4, which is characterized in that the material of the capsule is permeable thermoplastic poly
Close object, selected from high density polyethylene (HDPE), low density polyethylene (LDPE), ethylene vinyl acetate, polyvinyl chloride, thermoplastic polyurethane, polyethers/
At least one of amide block copolymers, preferably polyvinyl chloride.
6. controlled release system according to claim 4, which is characterized in that the particulate inert carrier is mineral or plant base
Carrier, selected from vermiculite, kaolin, bentonite, atlapulgite, sepiolite, White Carbon black, diatomite, precipitated calcium carbonate, talcum powder,
At least one of the plaster of paris, chalky soil, clay, montmorillonite, clay, high-purity soil, wood powder, bamboo powder, corn ear, preferably vermiculite.
7. controlled release system according to claim 6, which is characterized in that the partial size of the particulate inert carrier is 0.1-
10.0mm, preferably 2.1-3.0mm.
8. according to the described in any item controlled release systems of claim 4-7, which is characterized in that the surface of the sustained-release granular formulation is equipped with
Coating layer, coated fertilizer are selected from least one of polyethylene oxide, polyethylene glycol, stearic acid, paraffin, rosin, preferably polycyclic
Oxidative ethane.
9. controlled release system according to claim 8, which is characterized in that microbicide compositions in the sustained-release granular formulation,
The weight ratio of granular inert carrier and coated fertilizer is 1-50: 50-90: 5-10, preferably 35: 57.5: 7.5.
10. any one of any one of the claim 1-3 microbicide compositions or claim 4-9 controlled release system is being prevented and treated
Application in phytophthora pathogen and its caused plant disease；
Wherein, the phytophthora pathogen includes phytophthora infestans.
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| CN111838153A (en) * | 2020-07-02 | 2020-10-30 | 华中农业大学 | Biological control method for fruit and vegetable penicilliosis pathogenic bacteria |
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