CN110074137A - Application of the bacillus subtilis in prevention and treatment wheat aphid - Google Patents
Application of the bacillus subtilis in prevention and treatment wheat aphid Download PDFInfo
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- CN110074137A CN110074137A CN201910513266.9A CN201910513266A CN110074137A CN 110074137 A CN110074137 A CN 110074137A CN 201910513266 A CN201910513266 A CN 201910513266A CN 110074137 A CN110074137 A CN 110074137A
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- biocontrol agent
- subtilis
- bacillus subtilis
- aphid
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- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 44
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 28
- 241000125167 Rhopalosiphum padi Species 0.000 title claims abstract description 18
- 230000002265 prevention Effects 0.000 title description 8
- 239000012681 biocontrol agent Substances 0.000 claims abstract description 45
- 241000209140 Triticum Species 0.000 abstract description 29
- 235000021307 Triticum Nutrition 0.000 abstract description 29
- 230000003449 preventive effect Effects 0.000 abstract description 9
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 241001124076 Aphididae Species 0.000 description 12
- NWWZPOKUUAIXIW-DHZHZOJOSA-N (E)-thiamethoxam Chemical compound [O-][N+](=O)/N=C/1N(C)COCN\1CC1=CN=C(Cl)S1 NWWZPOKUUAIXIW-DHZHZOJOSA-N 0.000 description 11
- 239000000126 substance Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 239000002068 microbial inoculum Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 241000258937 Hemiptera Species 0.000 description 2
- 231100000674 Phytotoxicity Toxicity 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 235000007199 Panicum miliaceum Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241000180219 Sitobion avenae Species 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 244000022185 broomcorn panic Species 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bacillus subtilises to prepare the application in the biocontrol agent for preventing and treating wheat aphid, belongs to field of biological control.The invention also discloses a kind of biocontrol agents for preventing and treating wheat aphid comprising bacillus subtilis.Biocontrol agent of the invention can effectively prevent wheat aphid, and the preventive effect in wheat tillering latter stage has the function of promoting emergence and tiller respectively up to 85% or more, can promote and apply.
Description
Technical field
The present invention relates to field of biological control, and in particular, to application of the bacillus subtilis in prevention and treatment wheat aphid.
Background technique
Wheat is the main cereal crops in China, and aphid is common one of the wheat diseases in each producing region, with wheat leaf rust,
" a two sick worms " for powdery mildew and referred to as wheat.
Aphid is also known as aphid, aphid, is a kind of plant-feed insect, is one of most destructive pest on the earth.The world
Known about more than 4700, about 1100 kinds of distribution in China, wherein about 250 kinds are serious for agriculture and forestry and horticulture harm
Pest.The common wheat aphid in China has grain aphid, green bugs and broomcorn millet an ancient unit of weight equal to 20 or 24 *taels of silver pipe aphid, and the mixing of these three aphid parties occurs, long with wheat
Based on pipe aphid and green bugs.
Currently, wheat aphid is prevented and treated mainly by chemical pesticide, but chemical pesticide is also killed during killing pest
Its natural enemy insect can also cause pest resistance to insecticide to enhance, in addition, the application of chemical pesticide pollutes environment and influences human health,
Requirement especially with people to green instrument is continuously improved, and chemical prevention method is doubted.
Microbial insecticide has that environmental protection and public nuisance free, specificity and pathogenicity are strong, are difficult to generate relative to chemical insecticide
The characteristics of drug resistance, has been used for preventing and treating a variety of agriculture and forestry injurious insects.But in practice, still without using bacterium prevention and treatment wheat aphid
Report.
Summary of the invention
In order to solve the above-mentioned technical problem, the present invention is intended to provide a kind of application of bacterium in prevention and treatment wheat aphid.
This one side provides bacillus subtilis and is preparing the application in the biocontrol agent for preventing and treating wheat aphid.
Further, application according to claim 1, which is characterized in that the bacillus subtilis is selected from
B.subtilis NBRC 00101239 and B.subtilis VKM B-522.
Further, the bacillus subtilis is B.subtilis NBRC 00101239 and B.subtilis VKM
B-522。
Second aspect of the present invention provides a kind of biocontrol agent for preventing and treating wheat aphid comprising bacillus subtilis.
Further, the bacillus subtilis is selected from B.subtilis NBRC 00101239 and B.subtilis VKM
At least one of B-522.
In some embodiments of the present invention, the biocontrol agent is liquor.
Further, the content of bacillus subtilis is 2 × 10 in the biocontrol agent8A/mL.
In some embodiments of the present invention, the bacillus subtilis is 00101239 He of B.subtilis NBRC
B.subtilis VKM B-522。
In some specific embodiments of the invention, the bacillus subtilis is B.subtilis NBRC
The content of 0101239 and B.subtilis VKM B-522 is 1 × 10 respectively8A/mL.
Beneficial effects of the present invention
Biocontrol agent of the invention can effectively prevent wheat aphid, the preventive effect in wheat tillering latter stage respectively up to 85% with
On, and have the function of promoting emergence and tiller, it can promote and apply.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.
Embodiment
Following example is used herein to demonstration the preferred embodiments of the invention.Those skilled in the art, it will be appreciated that under
State the technology disclosed in example represent inventor discovery can be used for implementing technology of the invention, therefore can be considered as implementation this
The preferred embodiment of invention.But those skilled in the art should be understood that specific reality disclosed herein according to this specification
Many modifications can be made by applying example, still can be obtained identical or similar as a result, rather than away from the spirit or scope of the present invention.
Unless otherwise defined, the term of all technologies as used herein and science, and the technology in fields of the present invention
Personnel institute is normally understood equivalent in meaning, and being disclosed reference and their materials of reference will all be incorporated.
Those skilled in the art will recognize or just will appreciate that by routine test many described here
Invention specific embodiment many equivalent technologies.These will equally be comprised in claims.
The preparation of 1 biocontrol agent of embodiment
Biocontrol agent 1 includes bacillus subtilis Bacillus subtilis NBRC 00101239；Biocontrol agent 2 wraps
Include bacillus subtilis B.subtilis VKM B-522；Biocontrol agent 3 includes bacillus subtilis B.subtilis NBRC
00101239 and B.subtilis VKM B-522.
Respectively then fermentation liquid is centrifuged by bacillus subtilis through actication of culture, seed liquor culture, liquid fermentation, from
Sediment freeze-drying ultrafiltration instrument freeze-drying after the heart, is crushed into powder and obtains bacillus subtilis powder.
Above-mentioned bacterium powder is suitably diluted with sterile water, is then mixed by different proportion, so that the biocontrol agent 1 of preparation includes
Bacillus subtilis B.subtilis NBRC 00,101,239 2 × 108A/mL；Biocontrol agent 2 includes bacillus subtilis
B.subtilis VKM B-522 2×108A/mL；Biocontrol agent 1 includes bacillus subtilis B.subtilis NBRC
00101239 and B.subtilis VKM B-522 difference 1 × 108A/mL.
2 field efficiency test of embodiment
1 medicament
10% Diacloden microcapsule suspending agent (production of insecticide factory, University of Science and Technology, Xinyi City)
2 experimental plots
Test is carried out in experimental field, is to raise wheat 18 for examination wheat breed.Clay broadcasts sowing.Not using prevention and treatment aphid during test
The medicament of worm.Fertility is uniform, and wheat growing way is preferable.
3 experimental designs
Test sets 5 processing: the biocontrol agent 1 that 1. prepared by embodiment 1,20mL seed dressing 1kg；2. life prepared by embodiment 1
Anti- microbial inoculum 2,20mL seed dressing 1kg；3. biocontrol agent 3 prepared by embodiment 1,20mL seed dressing 1kg；4. 10% Diacloden CS 20mL
Dress seed 1kg；5. blank control.Each processing sets 4 repetitions, and random district's groups arrangement amounts to 20 cells, every plot area is
20m2。
4 spraying times and time
It dresses seed 1 time before wheat cultivation.Microbial inoculum, pharmaceutical preparation amount are first diluted with water into 4% medical fluid of seed weight, then
It is sufficiently stirred with seed, is sowed after medical fluid is evenly distributed on the surface of the seed, dries.
When every cell is sowed, first sow 100 and simultaneously covered with plastic film, etc. after cells sowing whole, then throw off
Film carries out mark (investigating emergence rate after the neat seedling of wheat).
The investigation of 5 preventive effects
3 preventive effects are investigated during test altogether.It is carried out respectively in wheat tillering latter stage, jointing stage, ear period.Every cell diagonal line
Fix at 5 points, look into 20 plants, and record aphid is total, and the calculating control efficiency compared with blank district at every.
In formula: CK-blank district aphid number；PT-microbial inoculum chemicals treatment district aphid number.
The results are shown in Table 1.
The different microbial inoculums of table 1, preparation are to the control efficiency of wheat aphid
According to the investigation result in wheat tillering latter stage, 3 kinds of microbial inoculums and a kind of chemicals treatment have obvious prevention and treatment effect to wheat aphid
Fruit.Application biocontrol agent 1, biocontrol agent 2, biocontrol agent 3, Diacloden seed dressing preventive effect be respectively 83.08%, 82.09%,
87.56% and 72.14%., it is apparent that the control efficiency of biocontrol agent is significantly higher than Diacloden.
It is investigated in wheat during jointing stage, the lower wheat aphid quantity of each processing rises, and 3 kinds of biocontrol agents and a kind of medicament are prevented
Controlling effect has downward trend compared with wheat tillering latter stage.Biocontrol agent 1, biocontrol agent 2, biocontrol agent 3, Diacloden seed dressing
Preventive effect be respectively 71.66%, 70.78%, 77.55%, 52.39% (table 1).
The survey showed that for wheat earing stage, and Diacloden Dressing preventive effect is worst, and preventive effect only has 44.38%.On the contrary, raw
Anti- microbial inoculum processing still has relatively high control efficiency.Wherein, the control efficiency of mix bacterium agent is best, up to 76.63%.
6 security surveys
Wheat Qi Miaohou, it is overwintering before, late tillering state, the jointing stage observation medicament to crop, whether there is or not phytotoxicity, record the class of phytotoxicity
Type and degree.
According to wheat Qi Miaohou, late tillering state, jointing stage observation, each microbial inoculum processing does not cause bad shadow to wheat growth
It rings, without obvious symptom of chemical damage.Show to handle wheat seeds by operation requirement under test dose for examination microbial inoculum, to wheat growth safety.
The influence of 7 pairs of wheat growths
Emergence rate is investigated after the neat seedling of wheat.Tiller situation is investigated in late tillering state.5 points of every cell grab sample, every point
10 plants, tiller number is counted, calculates single plant tiller number.The results are shown in Table 2.
Influence of 2 different disposal of table to wheat growth
| Processing | Emergence rate (%) | Single plant tiller number (a/strain) |
| Biocontrol agent 1 | 80.3 | 1.57 |
| Biocontrol agent 2 | 78.4 | 1.63 |
| Biocontrol agent 3 | 86.9 | 1.79 |
| Diacloden | 70.6 | 1.01 |
| Blank control | 69.8 | 0.95 |
From the investigation result (table 2) of emergence rate it is found that compared with blank control, 3 kinds of biocontrol agents can significantly improve small
Wheat emergence rate, and Diacloden has not significant impact wheat seeding rate.Wheat Qi Miaohou, biocontrol agent 1, biocontrol agent 2, biological and ecological methods to prevent plant disease, pests, and erosion
The emergence rate that microbial inoculum 3, Diacloden, blank control are handled is respectively 80.3%, 78.4%, 86.9%, 70.6%, 69.8%.Table
Bright biocontrol agent can significantly improve wheat seeding rate, have certain stratification effect.
In wheat tillering latter stage, what biocontrol agent 1, biocontrol agent 2, biocontrol agent 3, Diacloden, blank control were handled is averaged
Single plant tiller number is respectively 1.57,1.63,1.79,1.01,0.95, the single plant tiller number of each biocontrol agent processing
It is more than blank control, and Diacloden and blank control difference are unobvious.
The above result shows that biocontrol agent of the invention can effectively prevent wheat aphid, the preventive effect in wheat tillering latter stage
Respectively up to 85% or more, and have the function of promoting emergence and tiller, can promote and apply.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (8)
1. bacillus subtilis is preparing the application in the biocontrol agent for preventing and treating wheat aphid.
2. application according to claim 1, which is characterized in that the bacillus subtilis is selected fromB. subtilis NBRC
00101239 HeB. subtilis VKM B-522。
3. application according to claim 2, which is characterized in that the bacillus subtilis isB. subtilis NBRC
00101239 HeB. subtilis VKM B-522。
4. a kind of biocontrol agent for preventing and treating wheat aphid, which is characterized in that including bacillus subtilis.
5. biocontrol agent according to claim 4, which is characterized in that the bacillus subtilis is selected fromB. subtilis
00101239 He of NBRCB. subtilis At least one of VKM B-522.
6. biocontrol agent according to claim 5, which is characterized in that the biocontrol agent is liquor.
7. biocontrol agent according to claim 6, which is characterized in that the content of bacillus subtilis in the biocontrol agent
It is 2 × 108A/mL.
8. biocontrol agent according to claim 5, which is characterized in that the bacillus subtilis isB. subtilis
00101239 He of NBRCB. subtilis VKM B-522。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910513266.9A CN110074137B (en) | 2019-06-14 | 2019-06-14 | Application of bacillus subtilis in prevention and treatment of wheat aphids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910513266.9A CN110074137B (en) | 2019-06-14 | 2019-06-14 | Application of bacillus subtilis in prevention and treatment of wheat aphids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110074137A true CN110074137A (en) | 2019-08-02 |
| CN110074137B CN110074137B (en) | 2020-03-17 |
Family
ID=67424282
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910513266.9A Active CN110074137B (en) | 2019-06-14 | 2019-06-14 | Application of bacillus subtilis in prevention and treatment of wheat aphids |
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| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111109298A (en) * | 2019-12-20 | 2020-05-08 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent with aphid prevention and treatment function and preparation method thereof |
| CN113265351A (en) * | 2021-05-11 | 2021-08-17 | 昆明理工大学 | Lactobacillus W8172 and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554240A (en) * | 2003-12-26 | 2004-12-15 | 南京农业大学 | Bacillus subtilis lipo-peptide biological pesticide and use |
| CN1556195A (en) * | 2003-12-30 | 2004-12-22 | 江苏省农业科学院 | Culturing method of sea source bacillus subtilis insect killing activity product and its application |
| CN105851065A (en) * | 2016-04-29 | 2016-08-17 | 山东胜伟园林科技有限公司 | Bacillus subtilis insecticide for preventing and controlling wheat insects and preparing method thereof |
| CN108485997A (en) * | 2018-02-11 | 2018-09-04 | 浙江大学 | A kind of method that biological control cuts down Wolfberry Aphid and improves production performance |
-
2019
- 2019-06-14 CN CN201910513266.9A patent/CN110074137B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554240A (en) * | 2003-12-26 | 2004-12-15 | 南京农业大学 | Bacillus subtilis lipo-peptide biological pesticide and use |
| CN1556195A (en) * | 2003-12-30 | 2004-12-22 | 江苏省农业科学院 | Culturing method of sea source bacillus subtilis insect killing activity product and its application |
| CN105851065A (en) * | 2016-04-29 | 2016-08-17 | 山东胜伟园林科技有限公司 | Bacillus subtilis insecticide for preventing and controlling wheat insects and preparing method thereof |
| CN108485997A (en) * | 2018-02-11 | 2018-09-04 | 浙江大学 | A kind of method that biological control cuts down Wolfberry Aphid and improves production performance |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111109298A (en) * | 2019-12-20 | 2020-05-08 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent with aphid prevention and treatment function and preparation method thereof |
| CN113265351A (en) * | 2021-05-11 | 2021-08-17 | 昆明理工大学 | Lactobacillus W8172 and application thereof |
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| Publication number | Publication date |
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| CN110074137B (en) | 2020-03-17 |
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