CN110150382A - 一种荞麦酸奶及其制备方法 - Google Patents
一种荞麦酸奶及其制备方法 Download PDFInfo
- Publication number
- CN110150382A CN110150382A CN201910525234.0A CN201910525234A CN110150382A CN 110150382 A CN110150382 A CN 110150382A CN 201910525234 A CN201910525234 A CN 201910525234A CN 110150382 A CN110150382 A CN 110150382A
- Authority
- CN
- China
- Prior art keywords
- buckwheat
- preparation
- yoghourt
- milk
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000219051 Fagopyrum Species 0.000 title claims abstract description 94
- 235000009419 Fagopyrum esculentum Nutrition 0.000 title claims abstract description 94
- 235000013618 yogurt Nutrition 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 235000013336 milk Nutrition 0.000 claims abstract description 21
- 239000008267 milk Substances 0.000 claims abstract description 21
- 210000004080 milk Anatomy 0.000 claims abstract description 21
- 239000002002 slurry Substances 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims abstract description 20
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 238000005213 imbibition Methods 0.000 claims abstract description 13
- 235000021262 sour milk Nutrition 0.000 claims abstract description 11
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 9
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 9
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 7
- HZUKSQHMCTUZJL-UHFFFAOYSA-N P(=O)(O)(O)OCC=1C(=C(C(=NC1)C)O)C=O.P(=O)(O)(O)OC=1C(=NC=C(C1C=O)CO)C Chemical compound P(=O)(O)(O)OCC=1C(=C(C(=NC1)C)O)C=O.P(=O)(O)(O)OC=1C(=NC=C(C1C=O)CO)C HZUKSQHMCTUZJL-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 7
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 7
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 7
- 239000007921 spray Substances 0.000 claims abstract description 7
- 239000000049 pigment Substances 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 230000035784 germination Effects 0.000 claims description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 238000004321 preservation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 12
- 238000000265 homogenisation Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 244000057717 Streptococcus lactis Species 0.000 claims description 5
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 5
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 5
- 229940009289 bifidobacterium lactis Drugs 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 239000003995 emulsifying agent Substances 0.000 claims description 2
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 239000002562 thickening agent Substances 0.000 claims description 2
- 240000001929 Lactobacillus brevis Species 0.000 claims 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 claims 1
- 244000199866 Lactobacillus casei Species 0.000 claims 1
- 235000013958 Lactobacillus casei Nutrition 0.000 claims 1
- 239000005708 Sodium hypochlorite Substances 0.000 claims 1
- 244000269722 Thea sinensis Species 0.000 claims 1
- 235000009508 confectionery Nutrition 0.000 claims 1
- 229940017800 lactobacillus casei Drugs 0.000 claims 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 abstract description 18
- 229960003692 gamma aminobutyric acid Drugs 0.000 abstract description 17
- 240000006162 Chenopodium quinoa Species 0.000 abstract description 10
- 239000002994 raw material Substances 0.000 abstract description 6
- 235000008935 nutritious Nutrition 0.000 abstract description 5
- 230000003020 moisturizing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 16
- 235000013339 cereals Nutrition 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 229940039696 lactobacillus Drugs 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 4
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- KQPBSBAEBKRAAU-UHFFFAOYSA-N hypochlorous acid;sodium Chemical compound [Na].ClO KQPBSBAEBKRAAU-UHFFFAOYSA-N 0.000 description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 4
- 239000004745 nonwoven fabric Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000976924 Inca Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000001212 derivatisation Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229940054441 o-phthalaldehyde Drugs 0.000 description 2
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000007974 sodium acetate buffer Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- QWCKQJZIFLGMSD-UHFFFAOYSA-N 2-Aminobutanoic acid Natural products CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 description 1
- 241000554155 Andes Species 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- QWCKQJZIFLGMSD-GSVOUGTGSA-N D-alpha-aminobutyric acid Chemical compound CC[C@@H](N)C(O)=O QWCKQJZIFLGMSD-GSVOUGTGSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000015140 cultured milk Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1315—Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/121—Brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Cereal-Derived Products (AREA)
Abstract
本发明提供了一种荞麦酸奶的制备方法:荞麦在营养液中浸泡吸胀;漂洗后,置于发芽床上,8‑10℃避光催芽,喷施营养液保湿;荞麦胚芽长与种子等长后,加水粉碎成荞麦浆;将荞麦浆加入α‑淀粉酶糖化获得荞麦汁;最后将牛奶、荞麦汁混合灭菌后,加入酸奶发酵剂,获得酸奶。所述营养液含11‑18mM谷氨酸钠、2‑2.5mM磷酸吡哆醛和0.75‑0.9mM氯化钙。本发明的酸奶以发芽藜麦为原料,经过糖化后与牛奶共同发酵,改善了牛奶的口感、丰富了酸奶中的GABA含量。
Description
技术领域
本发明属于食品领域,涉及一种含有荞麦成分的酸奶。
背景技术
藜麦原产于南美洲安第斯山区,是印加土著居民的主要传统食物,有5000-7000多年的食用和种植历史,由于其具有独特的营养价值,古代印加人称之为"粮食之母"。安第斯山的居民就将藜麦驯化种植,将其作为传统主食。古代印加人甚至称藜麦为“粮食之母”。藜麦不仅含有种类丰富的蛋白质、淀粉、矿物质、维生素等基本营养物质,黄酮类、多酚类、皂苷等功能性成分的含量也很高,还具有降血糖、抗氧化、缓解炎症、减脂等多种生理活性,是最适宜人类的全营养食品。
复合型酸奶是在牛奶中混入具有一定的营养保健功能或特殊风味原料发酵而成的产品。目前,研制具有特殊风味和营养保健作用的混合原料发酵酸牛乳新品种已成为我国酸乳产品开发的重要方面。目前市场上有诸多含谷物的奶制品,其制备方法主要是往成品酸奶中直接添加谷物。谷物中某些蛋白不易被消化利用。而谷物萌发过程中不仅发生了许多生理代谢变化,如酶的活化生成、细胞生理活性的恢复。同时可以提高谷物的营养品质,如改善氨基酸的组成、提高蛋白质的利用率、增加B族维生素的含量、降低抗营养因子等。萌芽的谷物中的营养物质更适于人体吸收和利用。
发明内容
针对目前含谷类酸奶营养物质不易吸收的问题,本发明提供一种荞麦酸奶,利用萌芽的荞麦为原料进行混合发酵,增加营养物质的含量。
本发明的另一目的是提供一种上述荞麦酸奶的制备方法。
为实现上述目的,本发明采用如下技术方案。
一种荞麦酸奶的制备方法,包括以下步骤:
(1)净选后的荞麦杀菌,在营养液中浸泡6-8h吸胀;
(2)将吸胀后的荞麦清水漂洗后,置于发芽床上,8-10℃避光催芽,中途多次喷施营养液保证纸床湿润;
(3)荞麦胚芽长与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水漂洗后,加入水粉碎成荞麦浆;
(4)将荞麦浆65-72℃加热保温60-120 min;然后加入α-淀粉酶混合均匀,50-60℃保温1-1.5 h;然后高压灭菌,获得荞麦汁;
(5)将牛奶、荞麦汁混合后选择性加入辅料,进行高压匀质,灭菌后,加入酸奶发酵剂,于40-45℃中保温发酵至pH为4.0-4.5,获得酸奶。
所述荞麦的发芽率在95%以上。
步骤(1)中,杀菌步骤为0.1%次氯酸钠溶液浸泡15-20min后,用清水冲洗2-3次。
步骤(1)和(2)中,所述营养液含11-18mM谷氨酸钠、2-2.5mM磷酸吡哆醛和0.75-0.9mM氯化钙。
步骤(3)中,所述水与荞麦种子的质量比为1:1-2。所述荞麦浆的细度为过100-120目。
步骤(4)中,所述α-淀粉酶的酶活为20万U/g,添加量为荞麦浆重的0.01%-0.03%。
步骤(5)中,所述荞麦汁的添加量为15-20%。所述酸奶发酵剂为含有保加利亚乳杆菌Lactobacillus bulgaricus、嗜酸乳杆菌Lactobacillus acidophilus、嗜热链球菌Streptococcus thermophilus、植物乳杆菌Lactobacillus plantarum、乳酸乳球菌Lactococcus lactis、短乳杆菌Lactobacillus brevis、乳双歧杆菌Bifidobacterium lactis、干酪乳杆菌Lactobacillus casei中至少一种的菌剂。
步骤(5)中,所述辅料选自增稠剂、甜味剂、乳化剂中的一种或几种。
一种上述方法制备的酸奶。
本发明具有以下有益效果:
将发芽藜麦为原料进行糖化,一方面利用藜麦萌发过程中自身酶的作用将淀粉、脂肪等储备能源转化为糖、减少糖化酶的用量;另一方面发芽藜麦中的蛋白被部分分解,转化成氨基酸,增加酸奶中游离氨基酸的含量,易消化;再一方面能够避免酸奶生产中额外添加糖。在藜麦发芽时,采用营养液诱导和低温诱导的方法,大大增加了酸奶中GABA的含量;同时结合乳酸菌的作用进一步发酵产生GABA。采用藜麦自身吸收的营养液中的成分为底物和促进物质,而不在牛奶中添加刺激GABA的物质,更加安全,尤其是可以避免底物过量影响酸奶口感。
本发明的酸奶以发芽藜麦为原料,经过糖化后与牛奶共同发酵,改善了牛奶的口感、丰富了酸奶中的GABA含量。
具体实施方式
下面结合实施例对本发明做进一步说明,但本发明不受下述实施例的限制。
实施例1 荞麦酸奶的制备
(1)将发芽率95%以上的荞麦种子剔除破碎、秕谷、霉变的进行净选,然后用0.1%次氯酸钠溶液浸泡15min,在用清水冲洗3次,每次5min左右,称取5kg,在营养液(含谷氨酸钠16.5mM、磷酸吡哆醛2.2mM和氯化钙0.85mM)中浸泡6h吸胀;
(2)将灭菌无纺布铺于网床上,上铺两层灭菌营养液饱和的吸水纸,完成发芽床铺设,然后将吸胀后的荞麦清水漂洗3次,置于发芽床上,8-10℃避光催芽,每隔2h喷施营养液,保持纸床湿润;
(3)待80%以上的荞麦胚芽长度与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水冲洗3次,沥干水分然后加入10kg纯净水磨成100-120目的荞麦浆;
(4)将荞麦浆72℃加热保温60 min;然后加入浆重0.02%的耐热α-淀粉酶(20万U/g)混合均匀,60℃保温1 h;然后115℃高压灭菌20min,获得荞麦汁;
(5)将50kg牛奶、10kg荞麦汁混合后,在25MPa的压力下进行高压匀质,高温瞬时灭菌后,按照1g/kg加入酸奶发酵剂,于43℃中保温发酵至pH为4.3左右,获得荞麦酸奶;
所述酸奶发酵剂为商业购买产品,含有保加利亚乳杆菌、嗜酸乳杆菌、嗜热链球菌、植物乳杆菌、乳酸乳球菌、鼠李糖乳杆菌、乳双歧杆菌。
对比例1 荞麦酸奶的制备
将发芽率95%以上的荞麦种子剔除破碎、秕谷、霉变的进行净选,取5kg,清水冲洗3次,沥干水分然后加入10kg纯净水磨成100-120目的荞麦浆;然后按照实施例1中步骤(4)-(5)发酵获得荞麦酸奶。
对比例2 荞麦酸奶的制备
按照实施例1的方法,将营养液替换为清水,发芽温度为25℃,制备获得酸奶。
对比例3 荞麦酸奶的制备
(1)按照对比例2中的方法获得荞麦汁;
(2)将50kg牛奶在25MPa的压力下进行高压匀质,高温瞬时灭菌后,按照1g/kg加入酸奶发酵剂,于43℃中保温发酵至pH为4.3左右,获得纯奶发酵酸奶;
(3)将荞麦汁与纯奶发酵酸奶混合后获得荞麦酸奶。
实施例3 不同酸奶中γ-氨基丁酸含量的检测
采用衍生法HPLC测定不同样品中GABA含量:
待测样品制备:分别取实施例1与对比例中获得酸奶产品5 mL于试管内,加入等体积质量分数为5% TCA溶液置于60℃恒温水浴锅中水浴2h,10000r/min离心10 min,取上清,用质量分数5% TCA溶液定容至5 mL,过0.22μm滤膜,待检测用;
标准品溶液的配制:准确称量5.0 mg γ-氨基丁酸标品置5 mL容量瓶中,用纯净水定容至5mL,使用时稀释成7个不同浓度过0.22μm滤膜,待检测用;
衍生剂配制:邻苯二甲醛(OPA)80.0 mg,β-巯基乙醇80μL,甲醇10 mL。
按照以下色谱条件进行HPLC检测:
色谱柱:Angilent氨基酸专用分析柱(5μm,4.6 mm×150 mm)、Angilent分析保护柱(5μm,4.6mm×12.5 mm);柱温25℃;
流动相:A:0.16wt%乙酸钠缓冲液,pH=7.15;B:0.16wt%乙酸钠缓冲液:甲醇:乙腈=1:4.5:4.5v/v/v,pH=7.15;
流速:1mL/min;
检测波长:334 nm;
进样量:2μL;
使用Angilent自动衍生进样程序:硼酸缓冲液10μL,样品2μL,混合3次,洗针,取衍生剂2μL,洗针,共14μL衍生液混合15次,进样;
梯度洗脱:0-2 min,体积分数20%流动相B;2-18 min,体积分数20%-60%流动相B;18-19min,体积分数60%-20%流动相B;19-25 min,体积分数20%流动相B。
根据峰面积计算不同样品中GABA的含量,结果如表1所示:
表1 酸奶中GABA含量
由表1中不同酸奶样品中GABA含量可知,与直接添加荞麦浆相比,添加发芽荞麦浆发酵的酸奶和直接添加发芽荞麦浆酸奶中的GABA含量大幅度增加,说明发芽荞麦酸奶中GABA含量提高;发芽荞麦与牛奶共发酵时可以有效提高成品中GABA的含量;与添加常规发芽荞麦浆相比,添加低温下以营养液催芽的荞麦浆的酸奶中GABA含量高近2倍。
实施例4 荞麦酸奶的制备
(1)将发芽率95%以上的荞麦种子剔除破碎、秕谷、霉变的进行净选,然后用0.1%次氯酸钠溶液浸泡15min,在用清水冲洗3次,每次5min左右,称取5kg,在营养液(含谷氨酸钠11mM、磷酸吡哆醛2mM和氯化钙0.75mM)中浸泡8h吸胀;
(2)将灭菌无纺布铺于网床上,上铺两层灭菌营养液饱和的吸水纸,完成发芽床铺设,然后将吸胀后的荞麦清水漂洗3次,置于发芽床上,8-10℃避光催芽,每隔2h喷施营养液,保持纸床湿润;
(3)待80%以上的荞麦胚芽长度与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水冲洗3次,沥干水分然后加入5kg纯净水磨成100-120目的荞麦浆;
(4)将荞麦浆65℃加热保温100 min;然后加入浆重0.02%的耐热α-淀粉酶(20万U/g)混合均匀, 60℃保温1.5h;然后115℃高压灭菌20 min,获得荞麦汁;
(5)将50kg牛奶、10kg荞麦汁混合后,在25MPa的压力下进行高压匀质,高温瞬时灭菌后,按照1g/kg加入酸奶发酵剂(含有保加利亚乳杆菌、嗜酸乳杆菌、嗜热链球菌、植物乳杆菌、乳酸乳球菌、鼠李糖乳杆菌、乳双歧杆菌),于43℃中保温发酵至pH为4.0左右,获得酸奶,GABA含量为50.02mg/100g。
实施例5 荞麦酸奶的制备
(1)将发芽率95%以上的荞麦种子剔除破碎、秕谷、霉变的进行净选,然后用0.1%次氯酸钠溶液浸泡15min,在用清水冲洗3次,每次5min左右,称取5kg,在营养液(含谷氨酸钠15mM、磷酸吡哆醛2.5mM和氯化钙0.9mM)中浸泡6h吸胀;
(2)将灭菌无纺布铺于网床上,上铺两层灭菌营养液饱和的吸水纸,完成发芽床铺设,然后将吸胀后的荞麦清水漂洗3次,置于发芽床上,8-10℃避光催芽,每隔2h喷施营养液,保持纸床湿润;
(3)待80%以上的荞麦胚芽长度与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水冲洗3次,沥干水分然后加入8kg纯净水磨成100-120目的荞麦浆;
(4)将荞麦浆70℃加热保温90min;然后加入浆重0.02%的耐热α-淀粉酶(20万U/g)混合均匀,60℃保温1.5h;然后115℃高压灭菌20 min,获得荞麦汁;
(5)将50kg牛奶、9kg荞麦汁混合后,在25MPa的压力下进行高压匀质,高温瞬时灭菌后,按照1g/kg加入酸奶发酵剂(含有保加利亚乳杆菌、嗜酸乳杆菌、嗜热链球菌、植物乳杆菌、乳酸乳球菌、鼠李糖乳杆菌、乳双歧杆菌),于43℃中保温发酵至pH为4.3左右,获得酸奶,GABA含量为51.68mg/100g。
实施例6 荞麦酸奶的制备
(1)将发芽率95%以上的荞麦种子剔除破碎、秕谷、霉变的进行净选,然后用0.1%次氯酸钠溶液浸泡15min,在用清水冲洗3次,每次5min左右,称取5kg,在营养液(含谷氨酸钠18mM、磷酸吡哆醛2mM和氯化钙0.75mM)中浸泡6h吸胀;
(2)将灭菌无纺布铺于网床上,上铺两层灭菌营养液饱和的吸水纸,完成发芽床铺设,然后将吸胀后的荞麦清水漂洗3次,置于发芽床上,8-10℃避光催芽,每隔2h喷施营养液,保持纸床湿润;
(3)待80%以上的荞麦胚芽长度与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水冲洗3次,沥干水分然后加入5kg纯净水磨成100-120目的荞麦浆;
(4)将荞麦浆65℃加热保温120 min;然后加入浆重0.02%的耐热α-淀粉酶(20万U/g)混合均匀,60℃保温1.5h;然后115℃高压灭菌20 min,获得荞麦汁;
(5)将50kg牛奶、5kg荞麦汁混合后,在25MPa的压力下进行高压匀质,高温瞬时灭菌后,按照1g/kg加入酸奶发酵剂(含有保加利亚乳杆菌、嗜酸乳杆菌、嗜热链球菌、植物乳杆菌、乳酸乳球菌、鼠李糖乳杆菌、乳双歧杆菌),于43℃中保温发酵至pH为4.0左右,获得酸奶,GABA含量为178.2mg/100g。
Claims (10)
1.一种荞麦酸奶的制备方法,其特征在于,包括以下步骤:
(1)净选后的荞麦杀菌,在营养液中浸泡6-8h吸胀;
(2)将吸胀后的荞麦清水漂洗后,置于发芽床上,8-10℃避光催芽,中途多次喷施营养液保证纸床湿润;
(3)荞麦胚芽长与种子等长后,将催芽荞麦筛选,去除发霉及未发芽的,清水漂洗后,加入水粉碎成荞麦浆;
(4)将荞麦浆65-72℃加热保温60-120 min;然后加入α-淀粉酶混合均匀,50-60℃保温1-1.5 h;然后高压灭菌,获得荞麦汁;
(5)将牛奶、荞麦汁混合后选择性加入辅料,进行高压匀质,灭菌后,加入酸奶发酵剂,于40-45℃中保温发酵至pH为4.0-4.5,获得酸奶。
2.根据权利要求1所述的制备方法,其特征在于,步骤(1)和(2)中,所述营养液含11-18mM谷氨酸钠、2-2.5mM磷酸吡哆醛和0.75-0.9mM氯化钙。
3.根据权利要求1所述的制备方法,其特征在于,荞麦的发芽率在95%以上。
4.根据权利要求1所述的制备方法,其特征在于,步骤(1)中,杀菌步骤为0.1%次氯酸钠溶液浸泡15-20min后,用清水冲洗2-3次。
5.根据权利要求1所述的制备方法,其特征在于,步骤(3)中,所述水与荞麦种子的质量比为1:1-2;所述荞麦浆的细度为过100-120目。
6.根据权利要求1所述的制备方法,其特征在于,步骤(4)中,所述α-淀粉酶的酶活为20万U/g,添加量为荞麦浆重的0.01%-0.03%。
7.根据权利要求1所述的制备方法,其特征在于,步骤(5)中,所述荞麦汁的添加量为15-20%。
8.根据权利要求1所述的制备方法,其特征在于,所述酸奶发酵剂为含有保加利亚乳杆菌、嗜酸乳杆菌、嗜热链球菌、植物乳杆菌、乳酸乳球菌、短乳杆菌、乳双歧杆菌、干酪乳杆菌中至少一种的菌剂。
9.根据权利要求1所述的制备方法,其特征在于,步骤(5)中,所述辅料选自增稠剂、甜味剂、乳化剂中的一种或几种。
10.一种如权利要求1-9任一制备方法获得的酸奶。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910525234.0A CN110150382A (zh) | 2019-06-18 | 2019-06-18 | 一种荞麦酸奶及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910525234.0A CN110150382A (zh) | 2019-06-18 | 2019-06-18 | 一种荞麦酸奶及其制备方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110150382A true CN110150382A (zh) | 2019-08-23 |
Family
ID=67625944
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910525234.0A Pending CN110150382A (zh) | 2019-06-18 | 2019-06-18 | 一种荞麦酸奶及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110150382A (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110915916A (zh) * | 2019-11-05 | 2020-03-27 | 山西中医药大学 | 一种酸奶中富集芦丁的方法 |
CN114246222A (zh) * | 2021-12-29 | 2022-03-29 | 宁波御坊堂生物科技有限公司 | 一种具有安神助眠、改善胃肠道功能的植物基酸奶及其制备方法 |
CN117137083A (zh) * | 2023-09-18 | 2023-12-01 | 吉林省茂熙农业科技有限公司 | 一种藜麦代餐粉及其制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104170973A (zh) * | 2014-09-15 | 2014-12-03 | 衡阳师范学院 | 一种五谷酸奶的制备方法 |
-
2019
- 2019-06-18 CN CN201910525234.0A patent/CN110150382A/zh active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104170973A (zh) * | 2014-09-15 | 2014-12-03 | 衡阳师范学院 | 一种五谷酸奶的制备方法 |
Non-Patent Citations (2)
Title |
---|
发酵型萌动苦荞麦酸奶的研究: "发酵型萌动苦荞麦酸奶的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
袁建等: "高γ-氨基丁酸发芽糙米工艺条件的优化研究", 《粮食与饲料工业》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110915916A (zh) * | 2019-11-05 | 2020-03-27 | 山西中医药大学 | 一种酸奶中富集芦丁的方法 |
CN114246222A (zh) * | 2021-12-29 | 2022-03-29 | 宁波御坊堂生物科技有限公司 | 一种具有安神助眠、改善胃肠道功能的植物基酸奶及其制备方法 |
CN114246222B (zh) * | 2021-12-29 | 2024-07-26 | 宁波御坊堂生物科技有限公司 | 一种具有安神助眠、改善胃肠道功能的植物基酸奶及其制备方法 |
CN117137083A (zh) * | 2023-09-18 | 2023-12-01 | 吉林省茂熙农业科技有限公司 | 一种藜麦代餐粉及其制备方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104522803B (zh) | 一种苦荞胚芽、燕麦胚芽复合发酵饮料及其制备方法 | |
CN110150382A (zh) | 一种荞麦酸奶及其制备方法 | |
CN106418089A (zh) | 一种藜麦发酵乳及其制备方法 | |
CN105794995A (zh) | 乳酸菌发酵黄浆水制备酸浆豆腐的工艺 | |
CN108094528A (zh) | 豆渣发酵粉的制备方法 | |
CN105831248A (zh) | 一种葛根黑豆酸奶 | |
CN108185006A (zh) | 一种营养增效型系列豆芽发酵乳饮料及其制备工艺 | |
CN107354057A (zh) | 一种马蹄甘蔗保健酒的制备工艺 | |
CN106721769A (zh) | 一种山药梨汁复合发酵饮料 | |
CN106107338A (zh) | 一种乳酸菌发酵甜玉米复合饮料及其制备方法 | |
CN105145824A (zh) | 一种薄荷清爽银耳乳酸饮料及其制备方法 | |
CN106070656A (zh) | 一种紫薯乳酸菌复合饮料及其制备方法 | |
CN105901203A (zh) | 具有降脂、调压、降血糖文冠果茯茶的制备方法 | |
CN103181520A (zh) | 一种铁皮石斛乳酸菌胶囊的制备方法 | |
CN101180992A (zh) | 一种养生膳食调味乳及其制备方法 | |
CN104694341B (zh) | 一种利用中温大曲生产的苦荞五粮黄酒及其制备方法 | |
CN108850170A (zh) | 一种红豆薏米酸奶及其制备方法 | |
CN106070623A (zh) | 一种绞股蓝发酵乳及其制备方法 | |
CN103431106B (zh) | 一种保健复合茶制作工艺 | |
CN104872771A (zh) | 一种采用复合乳酸菌发酵制备的果蔬型饮料及其制备方法 | |
CN109430476A (zh) | 具有通便润肠功能的茶酵素饮品及其制备方法 | |
CN109588708A (zh) | 一种地梢瓜发酵液及其制备方法和饮料 | |
KR102587979B1 (ko) | 젖산발효를 통한 기능성 상황버섯 발효물 및 이의 제조방법 | |
CN108576297A (zh) | 苦荞芽苗发酵茶饮料及其制作方法 | |
CN110139653A (zh) | 雄性激素增加促进用组合物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190823 |