CN110093339A - A kind of five seasons rich microbial inoculum and preparation method thereof - Google Patents
A kind of five seasons rich microbial inoculum and preparation method thereof Download PDFInfo
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- CN110093339A CN110093339A CN201910377078.8A CN201910377078A CN110093339A CN 110093339 A CN110093339 A CN 110093339A CN 201910377078 A CN201910377078 A CN 201910377078A CN 110093339 A CN110093339 A CN 110093339A
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- microbial inoculum
- bacterium solution
- liquid
- trichoderma harzianum
- streptomyces microflavus
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 57
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 36
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 35
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 33
- 239000002245 particle Substances 0.000 claims abstract description 28
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 239000010828 animal waste Substances 0.000 claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000002609 medium Substances 0.000 claims abstract description 5
- 230000011218 segmentation Effects 0.000 claims abstract description 5
- 239000012798 spherical particle Substances 0.000 claims abstract description 5
- 238000005406 washing Methods 0.000 claims abstract description 5
- 239000010812 mixed waste Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000008223 sterile water Substances 0.000 claims description 12
- 238000004879 turbidimetry Methods 0.000 claims description 11
- 238000010521 absorption reaction Methods 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 6
- 241000510097 Megalonaias nervosa Species 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
Abstract
The invention discloses rich microbial inoculums of a kind of five seasons and preparation method thereof, are related to microbial inoculum preparation related fields, need a kind of new microbe carrier to adsorb microbial inoculum to solve the problem of that common turf carrier in the prior art is non-renewable.In terms of material quantity ratio, including streptomyces microflavus 11~14%, trichoderma harzianum 22~25%, bacillus 11~14%, animal wastes 15~25% and stalk 32~36%, include the following steps: step 1: stalk segmentation being smashed, the stalk particle of different-grain diameter is made；Step 2: the stalk particle of different-grain diameter is mixed, and animal wastes mixing is added and is twisted into spherical particle until being uniformly mixed, microbe carrier is made；Step 3: streptomyces microflavus, trichoderma harzianum and bacillus are inoculated in the medium respectively, carry out shaken cultivation；Step 4: with the strain under sterile washing on culture medium, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid are prepared into.
Description
Technical field
The present invention relates to microbial inoculums to prepare related fields, specially rich microbial inoculum of a kind of five seasons and preparation method thereof.
Background technique
Microbial bacterial agent refers to objective microbe after industrialized production expansion is numerous, using porous substance as absorption
The active bacteria formulation that the fermentation liquid of thallus is processed into is adsorbed in agent.This microbial inoculum has and directly or indirectly changes for seed dressing or root dipping
Good soil restores soil fertility, prevention soil-borne disease, maintains the effects of rhizosphere microflora balance and degradation poisonous and harmful substances.Agriculture
With microbial bacterial agent appropriately using can be improved agricultural output, improve quality of agricultural product, reduce fertilizer amount, reduce cost,
Improvement soil is preserved the ecological environment.
Existing microbial inoculum mostly uses turf as adsorbent material, but natural turf is limited natural resources, non-renewable,
Therefore a kind of new carrier is needed to remove absorption microbial inoculum, and new carrier cannot destroy existing microorganism fungus kind；
Therefore it is existing to help people to solve the problems, such as to develop rich microbial inoculum of a kind of five seasons and preparation method thereof for market in urgent need.
Summary of the invention
The purpose of the present invention is to provide rich microbial inoculums of a kind of five seasons and preparation method thereof, to solve to mention in above-mentioned background technique
The problem of common turf carrier out is non-renewable, and a kind of new microbe carrier is needed to adsorb microbial inoculum.
To achieve the above object, the invention provides the following technical scheme: a kind of five seasons rich microbial inoculum and preparation method thereof, with material
Expect quantity than meter, including streptomyces microflavus 11~14%, trichoderma harzianum 22~25%, bacillus 11~14%, animal excreta
Just 15~25% and stalk 32~36%.
Preferably, include the following steps:
Step 1: stalk segmentation is smashed, the stalk particle of different-grain diameter is made；
Step 2: the stalk particle of different-grain diameter is mixed, and animal wastes mixing is added, until mixing is equal
It is even, it is twisted into spherical particle, microbe carrier is made；
Step 3: streptomyces microflavus, trichoderma harzianum and bacillus are inoculated in the medium respectively, carry out oscillation training
It supports；
Step 4: with the strain under sterile washing on culture medium, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bud are prepared into
Spore bacillus liquid；
Step 5: each bacterial concentration is adjusted by turbidimetry, keeps the capacity of each bacterium solution and clump count of substantially equal；
Step 6: streptomyces microflavus liquid adjusted, Trichoderma harzianum bacterium solution and bacillus liquid are mixed, are made micro-
Biological bacterium solution；
Step 7: microbe carrier and microbial inoculum are poured into mixing bag body, incubator is put into and is cultivated, is made micro-
Bacteria agent.
Preferably, it in the step 1, is screened before preparing microbe carrier, guarantees the stalk particle smashed maximum
Partial size be not more than 30mm, minimum grain size be not less than 10mm, the stalk particle partial size of different-grain diameter be preferably 10mm, 15mm, 20mm,
25mm and 30mm.
Preferably, in the step 2, stalk particle and animal wastes mixed proportion are 1:2, and animal wastes are before mixing
For moisture state, if frangible or not agglomerating after mixing, a small amount of repeatedly addition sterile water simultaneously stirs again, makes microbe carrier
Just agglomerating, distance is 30mm between washboard is maximum up and down in equipment of chopping the chop, and speed of chopping the chop keeps uniform.
Preferably, in the step 4, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution are identical with the capacity of bacillus liquid,
And make the total capacity of streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid compared between aim parameter there are capacity difference away from.
Preferably, in the step 5, during turbidimetry is adjusted, the transmitted light intensity of one of bacterium solution is first chosen
Degree and the ratio of incident intensity are adjusted other two kinds of bacterium solutions, turbidity is too small, then again with a small amount of nothing as standard value
Bacterium water washes the strain on lower culture medium again, pours into original bacterium solution, and capacity is adjusted to normal capacity, reuse turbidimetry into
It goes and adjusts, until the ratio and standard value of transmitted intensity and incident intensity are close, it is multiple on a small quantity to add if turbidity is excessive
Add sterile water, every addition is primary, the measuring and calculating of turbidity is just re-started, until the ratio and mark of transmitted intensity and incident intensity
Quasi- value is close.
Preferably, in the step 6, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid is concentrated and mixed
Afterwards, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid measuring cup are rinsed respectively using sterile water, and will rinsed
Liquid pours into microbial inoculum.
Preferably, in the step 7, the mixed proportion of microbe carrier and microbial inoculum makes microbial inoculum just
It is fully absorbed by microbe carrier, and the hole absorption of microbe carrier just reaches saturation state.
Compared with prior art, the beneficial effects of the present invention are:
1, the invention has micropore right by the way that stalk and animal wastes 1:2 are mixed and made into microbe carrier in stalk
Microbial inoculum is adsorbed well, and the easy acquisition of animal excreta, absorption property is good, and has the organic matters such as nitrogen, phosphorus, using dynamic
Object excrement mixes as jointing material with stalk agglomerating, so that microbe carrier itself is had certain nutriment, and have big
Metering-orifice gap, the adsorbed microbial inoculum of single microbial carrier is more, keeps strain content higher, is conducive to the need of plant growth
It wants；
2, in the invention, the stalk particle partial size of different-grain diameter is 10mm, 15mm, 20mm, 25mm and 30mm, passes through setting
The stalk particle of a variety of partial sizes keeps pockets of microbe carrier excessively real during chopping the chop, and is conducive to the absorption of bacterium solution；
3, in the invention, in streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid transfer centralized procedure, make thin
There are capacity differences away from then passing through nothing compared between aim parameter for the total capacity of yellow streptomycete liquid, Trichoderma harzianum bacterium solution and bacillus liquid
Bacterium water is rinsed measuring cup, can guarantee that content of microorganisms is more nearly with target content in microbial inoculum, and pass through ratio
Turbid method is adjusted, the variable of clump count in control streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid；
4, in the invention, streptomyces microflavus 11~14%, bacillus 11~14%, is moved trichoderma harzianum 22~25%
Object excrement 15~25% and stalk 32~36% make manufactured microbe carrier and microbial inoculum just be absorbed into saturation shape
State does not waste microbial inoculum, save the cost.
Specific embodiment
Below in conjunction with embodiment in the present invention, technical solution of the present invention is clearly and completely described, it is described
Embodiment be only a part of the embodiment of the present invention, instead of all the embodiments.
A kind of embodiment provided by the invention: a kind of five seasons rich microbial inoculum and preparation method thereof, in terms of material quantity ratio, including
Streptomyces microflavus 11~14%, trichoderma harzianum 22~25%, bacillus 11~14%, animal wastes 15~25% and straw
Stalk 32~36%.
Further, include the following steps:
Step 1: stalk segmentation is smashed, the stalk particle of different-grain diameter is made；
Step 2: the stalk particle of different-grain diameter is mixed, and animal wastes mixing is added, and is had in stalk
Micropore can adsorb well microbial inoculum, and the easy acquisition of animal excreta, absorption property is good, and organic with nitrogen, phosphorus etc.
Object uses animal wastes to mix as jointing material with stalk agglomerating, microbe carrier itself is made to have certain nutriment,
And there are a large amount of holes, the adsorbed microbial inoculum of single microbial carrier is more, keeps strain content higher, and it is raw to be conducive to plant
Long needs are twisted into spherical particle, microbe carrier are made until being uniformly mixed；
Step 3: streptomyces microflavus, trichoderma harzianum and bacillus are inoculated in the medium respectively, carry out oscillation training
It supports；
Step 4: with the strain under sterile washing on culture medium, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bud are prepared into
Spore bacillus liquid；
Step 5: each bacterial concentration, control streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and gemma bar are adjusted by turbidimetry
The variable of clump count in bacterium solution, keeps the capacity of each bacterium solution and clump count of substantially equal；
Step 6: streptomyces microflavus liquid adjusted, Trichoderma harzianum bacterium solution and bacillus liquid are mixed, are made micro-
Biological bacterium solution；
Step 7: microbe carrier and microbial inoculum are poured into mixing bag body, incubator is put into and is cultivated, is made micro-
Bacteria agent.
Further, it in step 1, is screened before preparing microbe carrier, guarantees the stalk particle maximum particle diameter smashed
No more than 30mm, minimum grain size is not less than 10mm, the stalk particle partial size of different-grain diameter be preferably 10mm, 15mm, 20mm,
25mm and 30mm, the stalk particle by the way that a variety of partial sizes are arranged make pockets of microbe carrier will not mistake during chopping the chop
It is real, be conducive to the absorption of bacterium solution.
Further, in step 2, stalk particle and animal wastes mixed proportion are 1:2, and animal wastes are wet before mixing
Profit state, if frangible or not agglomerating after mixing, a small amount of repeatedly addition sterile water simultaneously stirs again, makes microbe carrier just
Agglomerating, distance is 30mm between washboard is maximum up and down in equipment of chopping the chop, and speed of chopping the chop keeps uniform.
Further, in step 4, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution are identical with the capacity of bacillus liquid, and make
The total capacity of streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid compared between aim parameter there are capacity difference away from.
Further, in step 5, during turbidimetry is adjusted, first choose the transmitted intensity of one of bacterium solution with
The ratio of incident intensity is adjusted other two kinds of bacterium solutions as standard value, and turbidity is too small, then again with a small amount of sterile water
The strain on lower culture medium is washed again, is poured into original bacterium solution, capacity is adjusted to normal capacity, is reused turbidimetry and is adjusted
It is whole, until the ratio and standard value of transmitted intensity and incident intensity are close, if turbidity is excessive, a small amount of multiple addition nothing
Bacterium water, every addition is primary, just re-starts the measuring and calculating of turbidity, until the ratio and standard value of transmitted intensity and incident intensity
It is close.
Further, in step 6, after concentrating mixing to streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid, make
Streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid measuring cup are rinsed respectively with sterile water, and flushing liquor is fallen
Enter in microbial inoculum, can guarantee that content of microorganisms is more nearly with target content in microbial inoculum.
Further, in step 7, the mixed proportion of microbe carrier and microbial inoculum keeps microbial inoculum just micro-
Bio-carrier fully absorbs, and the hole absorption of microbe carrier just reaches saturation state.
Experimental result and conclusion: there is bacterium during expressing early period in microbial inoculum made of microbe carrier and microbial inoculum
The case where kind is reduced, microbial inoculum is also underused microbe carrier and is bred, micro- on microbial inoculum in late stage
Energy on biological utilisation microbe carrier carries out mass propagation, and wraps up root system of plant surface, and harmful bacteria is in root system of plant table
Face significantly reduces, and to using the plant of this microbial inoculum to carry out the long-term comparison for controlling variable with the plant that this microbial inoculum is not used, uses
The plant of this microbial inoculum grows more luxuriant substantially without insect pest phenomenon.
Working principle: in use, stalk segmentation is smashed, the stalk particle of different-grain diameter is made, guarantees the stalk smashed
Particle maximum particle diameter be not more than 30mm, minimum grain size be not less than 10mm, the stalk particle partial size of different-grain diameter be preferably 10mm,
15mm, 20mm, 25mm and 30mm mix the stalk particle of different-grain diameter, and animal wastes mixing, stalk is added
Particle and animal wastes mixed proportion are 1:2, until it is uniformly mixed, it is twisted into spherical particle, washboard is maximum up and down in equipment of chopping the chop
Between distance be 30mm, speed of chopping the chop keep uniformly, microbe carrier is made, by streptomyces microflavus, trichoderma harzianum and gemma bar
Bacterium is inoculated in the medium respectively, carries out shaken cultivation with the strain under sterile washing on culture medium and is prepared into streptomyces microflavus
Liquid, Trichoderma harzianum bacterium solution and bacillus liquid adjust each bacterial concentration by turbidimetry, and turbidity is too small, then again with a small amount of nothing
Bacterium water washes the strain on lower culture medium again, pours into original bacterium solution, and capacity is adjusted to normal capacity, reuse turbidimetry into
It goes and adjusts, until the ratio and standard value of transmitted intensity and incident intensity are close, it is multiple on a small quantity to add if turbidity is excessive
Add sterile water, every addition is primary, the measuring and calculating of turbidity is just re-started, until the ratio and mark of transmitted intensity and incident intensity
Quasi- value is close, keeps the capacity of each bacterium solution and clump count of substantially equal, by streptomyces microflavus liquid adjusted, Trichoderma harzianum bacterium solution
It is mixed with bacillus liquid, using sterile water respectively to streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid measure
Cup is rinsed, and flushing liquor is poured into microbial inoculum, microbial inoculum is made, by microbe carrier and microbial inoculum
Mixing bag body is poured into, incubator is put into and is cultivated, microbial bacterial agent is made.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
Claims (8)
1. rich microbial inoculum of a kind of five seasons and preparation method thereof, which is characterized in that in terms of material quantity ratio, including streptomyces microflavus 11~
14%, trichoderma harzianum 22~25%, bacillus 11~14%, animal wastes 15~25% and stalk 32~36%.
2. a kind of five seasons rich microbial inoculum according to claim 1 and preparation method thereof, which comprises the steps of:
Step 1: stalk segmentation is smashed, the stalk particle of different-grain diameter is made；
Step 2: the stalk particle of different-grain diameter is mixed, and animal wastes mixing is added, until be uniformly mixed,
It is twisted into spherical particle, microbe carrier is made；
Step 3: streptomyces microflavus, trichoderma harzianum and bacillus are inoculated in the medium respectively, carry out shaken cultivation；
Step 4: with the strain under sterile washing on culture medium, streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and gemma bar are prepared into
Bacterium solution；
Step 5: each bacterial concentration is adjusted by turbidimetry, keeps the capacity of each bacterium solution and clump count of substantially equal；
Step 6: streptomyces microflavus liquid adjusted, Trichoderma harzianum bacterium solution and bacillus liquid are mixed, microorganism is made
Bacterium solution；
Step 7: microbe carrier and microbial inoculum are poured into mixing bag body, incubator is put into and is cultivated, microorganism is made
Microbial inoculum.
3. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 1,
It is screened before preparing microbe carrier, guarantees the stalk particle maximum particle diameter smashed no more than 30mm, minimum grain size is not small
In the stalk particle partial size of 10mm, different-grain diameter be preferably 10mm, 15mm, 20mm, 25mm and 30mm.
4. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 2,
Stalk particle and animal wastes mixed proportion are 1:2, and animal wastes are moisture state before mixing, if frangible after mixing or not
It is agglomerating, then it a small amount of repeatedly addition sterile water and stirs again, keeps microbe carrier just agglomerating, upper and lower washboard is most in equipment of chopping the chop
Distance is 30mm between big, and speed of chopping the chop keeps uniform.
5. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 4,
Streptomyces microflavus liquid, Trichoderma harzianum bacterium solution are identical with the capacity of bacillus liquid, and make streptomyces microflavus liquid, Trichoderma harzianum bacterium solution
And the total capacity of bacillus liquid compared between aim parameter there are capacity difference away from.
6. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 5,
During turbidimetry is adjusted, the transmitted intensity of one of bacterium solution and the ratio of incident intensity are first chosen as standard
Value, is adjusted other two kinds of bacterium solutions, and turbidity is too small, then washes the strain on lower culture medium again with a small amount of sterile water again,
It pours into original bacterium solution, capacity is adjusted to normal capacity, reuse turbidimetry and be adjusted, until transmitted intensity and incidence
The ratio and standard value of luminous intensity are close, if turbidity is excessive, a small amount of multiple addition sterile water, every addition is primary, just again
The measuring and calculating of turbidity is carried out, until the ratio and standard value of transmitted intensity and incident intensity are close.
7. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 6,
After concentrating mixing to streptomyces microflavus liquid, Trichoderma harzianum bacterium solution and bacillus liquid, using sterile water respectively to streptomyces microflavus
Liquid, Trichoderma harzianum bacterium solution and bacillus liquid measuring cup are rinsed, and flushing liquor is poured into microbial inoculum.
8. a kind of five seasons rich microbial inoculum according to claim 2 and preparation method thereof, it is characterised in that: in the step 7,
The mixed proportion of microbe carrier and microbial inoculum fully absorbs microbial inoculum just by microbe carrier, and microorganism
The hole absorption of carrier just reaches saturation state.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114410518A (en) * | 2022-01-07 | 2022-04-29 | 春华秋实科技集团有限公司 | Compound microbial agent containing streptomyces and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410518A (en) * | 2022-01-07 | 2022-04-29 | 春华秋实科技集团有限公司 | Compound microbial agent containing streptomyces and application thereof |
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Application publication date: 20190806 |