CN110089634A - It is a kind of with enzymatic hydrolysis small peptide-additive complex compound feed and preparation method thereof - Google Patents
It is a kind of with enzymatic hydrolysis small peptide-additive complex compound feed and preparation method thereof Download PDFInfo
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- CN110089634A CN110089634A CN201810093476.2A CN201810093476A CN110089634A CN 110089634 A CN110089634 A CN 110089634A CN 201810093476 A CN201810093476 A CN 201810093476A CN 110089634 A CN110089634 A CN 110089634A
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- small peptide
- enzymatic hydrolysis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
- A23K10/28—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention provides the preparation method and corresponding feed that three kinds have enzymatic hydrolysis small peptide-additive complex compound feed.On the basis of original conventional feed, addition can form the additive of co-ordinate covalent bond with amino, vegetable protein and/or animal protein hydrolyze under the action of at least one protease becomes small peptide compound, include the amino with unoccupied orbital in small peptide compounds, the added additive that can form co-ordinate covalent bond and amino form complex, so that additive is more stable, overcomes it and keep in dark place, be afraid of oxygen, meet the shortcomings that soda acid is easy inactivation；On this basis, the additive of other classifications can also be added, the additive of other classifications can be formed in complex and be formed by cluster, so, the problem of being also just able to solve the stability of the additive of other classifications.
Description
Technical field
The invention belongs to field of feed processing, more particularly, to a kind of with enzymatic hydrolysis small peptide-additive complex compound feed
And preparation method thereof
Background technique
In the prior art, there are three types of processing method: one, by grease, albumen, whey powder Three feed raw material through Over emulsfication and
Homogeneous, then through drying, become solid powder；Two, by grease, albumen, whey powder, feed addictive through Over emulsfication, through being dried to
For the processing method of feedstuff；Three, grease, albumen, whey powder Three feed raw material one of (or in which) are passed through with enzyme
Homogeneous and emulsification and enzymatic hydrolysis are crossed, then through drying, becomes solid powder.
Process above is improving feed processing efficiency, and animal digestion is promoted to absorb, in terms of promoting feed emulsibility, dissolubility
Have the function of positive.But the first and second method have the shortcomings that one it is significant: the albumen in preparation-obtained feed
It is not hydrolyzed to small peptide, can not directly be absorbed by animal alimentary canal, biological value is low；There are small peptide, but small peptide in the third method
Content is lower, can not play the advantage of small peptide, and the absorption of feed addictive is also poor, can not carry out to feed addictive effective
Using, comprehensive utilization, the Potential performance of small peptide and feed addictive can not be also excavated, is a kind of greatly waste.
Summary of the invention
The first purpose of this invention is, aiming at the problems existing in the prior art, provides a kind of with enzymatic hydrolysis small peptide-
The preparation method of the feed of additive complex compound.
For this purpose, the present invention adopts the following technical scheme:
A kind of preparation method with enzymatic hydrolysis small peptide-additive complex compound feed, the preparation method include:
First method:
(1) it adds raw materials into water, emulsifier is added, then heating is added additive, is sufficiently stirred；The additive
Compound including co-ordinate covalent bond can be formed with amino；
(2) mixture obtained in step (1) is added in homogenizer and carries out homogenization；Or it will be in step (1)
Obtained mixture, which is added in mulser, carries out emulsification treatment；Or mixture obtained in step (1) is added to homogeneous
Homogenization is carried out in machine, and then the mixture after homogenization is added in mulser and carries out emulsification treatment；Or it will step
Suddenly mixture obtained in (1), which is added in mulser, carries out emulsification treatment, is then added to the mixture after emulsification treatment
Homogenization is carried out in homogenizer；Or according to raw material and the mixed viscosity situation of water, homogenizer is replaced with into colloid mill, or
The preceding road of homogenizer is arranged in colloid mill by person；
(3) semi-finished product obtained in step (2) are added to enzymatic vessel, enzyme is added, is carried out under certain temperature and pH value
Enzymatic hydrolysis, in the range of 20~100 DEG C, pH is controlled in the range of 1.5~12.0 heating and temperature control；
(4) semi-finished product that step (3) obtains are dried to powder has enzymatic hydrolysis small peptide-additive network to get to a kind of
Close the feed of object；
The raw material includes: that mass percent is 0~60% whey powder, 0~80% vegetable protein, 0~80%
Animal protein, 0~80% vegetable fat, 0~80% animal fat carry out mixing in proportion and are made into raw material, and at least exist
One of vegetable protein or animal protein.
Second method:
(1) it adds raw materials into water, heat and is sufficiently stirred；
(2) mixture obtained in step (1) is added in homogenizer and carries out homogenization；Or it will be in step (1)
Obtained mixture, which is added in mulser, to be handled；Or mixture obtained in step (1) is added in homogenizer
Homogenization is carried out, then the mixture after homogenization is added in mulser and is handled；Or it will be obtained in step (1)
To mixture be added in mulser and handled, then the mixture after emulsification treatment is added in homogenizer carry out it is equal
Matter processing；Or according to raw material and the mixed viscosity situation of water, homogenizer is replaced with into colloid mill, or colloid mill is arranged
In the preceding road of homogenizer；
(3) semi-finished product obtained in step (2) are added to enzymatic vessel, be added enzyme, at certain temperature and pH value into
Row enzymatic hydrolysis；In the range of 20~100 DEG C, pH is controlled in the range of 1.5~12.0 heating and temperature control；
(4) semi-finished product that step (3) obtains are added in another reactor tank, additive is then added, when stirring one section
Between；The additive includes the compound that co-ordinate covalent bond can be formed with amino；
(5) semi-finished product that step (4) obtains are dried to powder has enzymatic hydrolysis small peptide-additive network to get to a kind of
Close the feed of object；
The raw material includes: that mass percent is 0~60% whey powder, 0~80% vegetable protein, 0~80%
Animal protein, 0~80% vegetable fat, 0~80% animal fat carry out mixing in proportion and are made into raw material, and at least exist
One of vegetable protein or animal protein.
The third method:
On the basis of second method, by step (1) replace are as follows: add raw materials into water, be added emulsifier, then plus
Heat is simultaneously sufficiently stirred.
It preferably, is 0~60% whey powder, 0~60% vegetable protein, 0~50% animal groups by mass percent
Knit device pipe and its extract, 0~60% animal protein, 0~60% vegetable fat, 0~60% animal fat in proportion
It carries out mixing and is made into raw material, and at least there is one of vegetable protein or animal protein.
Preferably, the enzyme includes: at least one protease and carbohydrase, lipase, food flavor enzyme, cellulase, lactic acid bar
One of bacterium, Bifidobacterium Bifidum, hay bacillus, redox enzymes, isomerase, enzyme inhibitor, zymoexciter or a variety of groups
It closes.It that is to say and include at least protease.Protease is the general name of the class of enzymes of aminosal peptide chain.The effect of carbohydrase is cracking
By the combined chemical bond of monosaccharide in polysaccharide, polysaccharide is made to be degraded into lesser molecule.Lipase is under the jurisdiction of carboxyl ester hydrolysis
Enzyme, can gradually by triglyceride hydrolysis at glycerol and fatty acid.Redox enzymes is that a kind of catalytic oxidation-reduction is anti-
The enzyme answered can be divided into two class of oxidizing ferment and reductase.Lactobacillus genus lactic acid Bacteriaceae is ordered because sugar fermentation generates a large amount of lactic acid
Name.It exists extensively, acidophilia, and existence optimal pH is 5.5~6.0, certainly, still can give birth in the environment that pH is 3.0~4.5
It deposits, it is that acid-resistant strength is strongest in bactacin.Bifidobacterium Bifidum is the one kind isolated from the enteron aisle of breast milk nutrition youngster
The Gram-positive bacillus of anaerobism, hay bacillus are one kind of Bacillus.Isomerase is also known as allomerase, is catalysis life
At the general name of the enzyme of isomerization reaction.Enzyme inhibitor is that one kind with desmoenzyme and can reduce its active molecule.Due to inhibiting special
The activity for determining enzyme can kill the imbalance of pathogen or correction metabolism, and many related drugs are exactly enzyme inhibitor.Enzyme swashs
Agent living is a kind of substance that inactive enzyme can be enable to become active enzyme.
Additive includes: feed addictive and chemical combination specified in " People's Republic of China's catalogue of feed additive varieties "
Object, such as at least one of copper, iron, manganese, zinc, iodine, cobalt, molybdenum, selenium, nickel, vanadium, chromium, the soluble metallic salt of lithium；Such as sulfuric acid Asia
Iron, frerrous chloride, ferrous carbonate, iron chloride, sodium selenite, sodium selenate, copper sulphate, copper carbonate, copper chloride, manganese sulfate, carbonic acid
Manganese, manganese chloride, zinc carbonate, zinc sulfate, zinc chloride, potassium iodide, cobalt chloride, molybdate etc., additive can also include: dimension life
Plain A, vitamin D, vitamin E, vitamin K, vitamin C, vitamin B1, vitamin B2, vitamin B3, adenine phosphate, dimension life
Plain B5, vitamin B6, vitamin B11, vitamin B12, biotin etc., additionally include: fodder acidulant, flavouring agent etc.
Other additives specified in " catalogue of feed additive varieties ".
Second object of the present invention is, for the deficiencies in the prior art, provides a kind of with enzymatic hydrolysis small peptide-
The feed of additive complex compound.
For this purpose, above-mentioned purpose of the invention is achieved through the following technical solutions:
It is a kind of to be obtained according to prepared by the mentioned-above preparation method with enzymatic hydrolysis small peptide-additive complex compound feed
Feed.
Signified vegetable protein includes: pea protein, wheat gluten, barley protein, avenin, soybean concentration in the present invention
Albumen, rice protein, soybean protein isolate, textured soybean protein, dregs of beans, peanut protein, soy meal, peameal, broad bean powder, sesame
One of vegetable protein specified in the The Ministry of Agriculture of the People's Republic of China, MOA such as numb powder, peanut powder " feedstuff catalogue " is a variety of
Combination；Signified animal protein includes: fish meal, the molten slurry of fish, fish and its leftover bits and pieces, plasma protein, intestine membrane protein, whey egg
It is moved specified in the The Ministry of Agriculture of the People's Republic of China, MOA such as white, casein, lactoprotein, blood meal, hyperglobulinemia " feedstuff catalogue "
One of object albumen or a variety of combinations；Signified vegetable fat include: soybean oil, soybean lecithin, coconut oil, olive oil,
One of vegetable fat such as rice bran oil, sesame oil, sunflower oil, safflower oil, palm oil, rape oil, peanut oil, cotton oil are a variety of
Combination；Signified animal fat includes lard, chicken fat, duck oil, butter, sheep oil, cream etc. from the grease extracted in animal
One or more combinations；Signified animal tissue's device pipe and its extract includes animal intestinal tract, liver, heart, spleen, kidney
One of animal tissues' organ such as dirty, lungs, blood, muscle and their extract or a variety of combinations；Signified emulsification
Agent includes: acetic acid and fatty glyceride, lactic acid and fatty glyceride, cholic acid, lecithin, polyglycereol ricinoleate ester, gathers
Ethylene castor oleyl alcohol acid esters, casein sodium, polysorbate (sorbitan fatty acid rouge), tween, sorbitan stearic acid
Monoesters, fatty acid cane sugar ester etc. have combination one or more in the food and feed additive of emulsification function；Signified enzyme
Including protease and carbohydrase, lipase, food flavor enzyme, cellulase, Bacillus acidi lactici, Bifidobacterium Bifidum, hay bacillus, redox
Enzyme, isomerase etc. have one of substance, enzyme inhibitor and zymoexciter of biocatalysis or a variety of combinations.This
Signified homogenizer includes all machinery with homogeneous micronization function in invention, can also be mixed glutinous according to raw material and water
Situation is spent, homogenizer is replaced with into colloid mill, or colloid mill is arranged in the preceding working procedure of homogenizer, signified mulser
Including all machinery with emulsification function.
The principle of the invention lies in: on the basis of original conventional feed, addition can form co-ordinate covalent bond with amino
Additive, vegetable protein and/or animal protein hydrolyze under the action of at least one protease becomes small peptide compound, small
It is formed and is coordinated comprising the amino with unoccupied orbital, the added additive that can form co-ordinate covalent bond and amino in peptide compounds
Compound overcomes it and keeps in dark place, be afraid of oxygen, meet the shortcomings that soda acid is easy inactivation so that additive is more stable；On this basis,
The additive of other classifications can also be added, the additive of other classifications can be formed in complex and be formed by cluster
It is interior, so, the problem of being also just able to solve the stability of the additive of other classifications；In addition, the complex formed
Cluster can by other additives, such as: vitamin, microelement, acidulant and flavouring agent etc. be wrapped in its formation cluster
It is interior, to carry out protection appropriate to vitamin, microelement, acidulant and flavouring agent so that vitamin, microelement,
The stability of acidulant and flavouring agent is also improved；Furthermore it is also possible to grease is wrapped in wherein, so that this has enzymatic hydrolysis
Small peptide-additive complex compound feed will not generate oil bloom when giving animal feeding with water preparation.Simultaneously because enzymatic hydrolysis small peptide
It is hydrophilic protein, forms water-oil interface after being dissolved in intestinal juice into enteron aisle, lipase is most strong in water-oil interface activity, makes animal to rouge
Fat can preferably be absorbed.
The preparation method with enzymatic hydrolysis small peptide-additive complex compound feed provided by the present invention also has following excellent
Point:
(1) increase enzymolysis process, the degree of hydrolysis of albumen can be greatly improved, improve 25% or more, increase the small peptide of feed
Content absorbs small peptide directly by animal small intestine, consumes energy relatively low；
(2) feed addictive by emulsification after homogeneous, emulsification, first homogeneous, first emulsify after four kinds of methods of homogeneous to feed
Additive and small peptide albumen are mixed, and the uniformity is considerably beyond other stirring mixers, and well-known: feed addictive adds
Dosage is extremely small, only a ten thousandth even thousand a ten thousandths, time-consuming and laborious with conventional mixer, and mixed effect is bad.
Just solve the problems, such as that feed addictive mixing is non-uniform with this method.It moreover, can be small peptide albumen by this method
Little particle is bundled into feed addictive or small peptide microelement chelate, and by dry so that hydrophilic small peptide albumen and small
Go to the surface of particle while peptide-additive complex compound is with water evaporation, thus make animal preferential digestion absorb small peptide albumen,
Small peptide albumen-additive complex compound and the additive being contained in small peptide albumen-additive complex compound cluster.Institute of the present invention
Enzymatic hydrolysis small peptide-additive complex compound feed of offer improves animal to feed addictive utilization efficiency, reduction feed addictive
Dosage provides a kind of completely new method, it is known that: the feed addictives such as copper, iron, zinc, manganese excessively add in feed, meeting
It by fecal pollution soil, environment is not only influenced, goes back the health of the high risks mankind, law-making stipulation feed in China's adds
The upper limit of agent, some enterprises better culture efficiency in order to obtain, increases dosage privately, causes heavy metal-polluted in soil
Dye.Therefore, the service efficiency of animal feed additive can be improved in the present invention, and bigger use effect is played in same additive amount
Fruit.
(3) present invention can preferably wrap up feed addictive, it is known that: some feed addictives, which have, to be afraid of
Oxygen keeps in dark place, meets the shortcomings that soda acid easily loses activity, such as vitamin E, vitamin B complex, vitamin C, acidulant.At present
Feed addictive is wrapped up with micro- glue Nang embedding techniques, but embeds material and is all made of converted starch, maltodextrin, Arab
Glue, corn syrup, vegetable protein etc. do wall material, and molecular weight is larger, and it is not closed containment wall easily occur, light transmission ventilation occur, thus
Feed addictive is embedded cannot completely, so that its active component be made to disappear.Small peptide caused by the present invention is with two to three ammonia
Base acid composition, molecular weight is minimum, and feed addictive can be complexed, and makes to be fully sealed, and keeps coating more complete, makes loss of activity more
Less, more longlasting.
(4) content of ferro element and other feed addictives in lactation breast milk can be improved in the present invention, supplements anxious in sucking pig
The various nutrients needed improve sucking pig weight of weaning litter, and sucking pig feed before wean is eaten little, substantially based on breast milk, because
This, whether abundance seems is even more important for nutrition (especially feed addictive) in breast milk.All generally acknowledge in industry: milking sow
Mammary gland synthesis lactoprotein needed for essential amino acid both be from in the form of small peptide combine amino acid, and albumen be in breast milk most
Important nutriment, so, small peptide breast milk is formed it is especially important, can be with and together with feed addictive and small peptide be complexed
It is directly absorbed into body circulation by the micropore between intestinal mucosa cells, is largely converted to the mother rich in iron and other feed addictives
Cream.Enzymatic hydrolysis small peptide-additive complex compound feed provided by the present invention and preparation method thereof can be effectively small peptide and feeding
Feed additives are complexed together, improve the content of iron and other feed addictives in lactation breast milk, are badly in need of in supplement sucking pig each
Kind nutrient, while the small peptide of milking sow urgent need can be supplemented.
(6) feed of the invention can also be provided to wean sucking pig and the animal of other digestive organs undeveloped (such as aquatic products)
It is various to be rich in various absorption nutrients easy to digest, and high conversion rate, such as: due to placental barrier, come into being the storage of sucking pig iron compared with
Few, generally acknowledged three age in days intramuscular injection iron preparation of piglet is maximally efficient at present, but it is shorter to hold time after all.If intramuscular injection again,
Sucking pig weight gain, it is more time-consuming and laborious, and also causing to sucking pig stress.So supplementing iron and other battalion in the creep feed stage
Feeding element is the most effective method, since the ligand that iron or other feed addictives can be used as small peptide directly passes through intestines
Micropore enters Ti Repetition ring between Epithelial cell, the feed of enzymatic hydrolysis small peptide provided by the present invention and complex as feed additives and
Nutrient in the preparation-obtained feed of preparation method is easily digested and is converted, and the daily gain and material of sucking pig can be improved
Meat ratio.Similarly, prepared by feed of enzymatic hydrolysis small peptide provided by the present invention and complex as feed additives and preparation method thereof
To feed are made by meat cost and also has pole for the daily gain and reduction that improve aquatic products and the unsound animal of other digestive system developments
For significant effect.
(7) feed of the invention also has antibacterium virus, anti-oxidation function, can inhibit the virulence factor of bacterial virus,
Improve the resistivity of animal.To reduce Animal diseases, animal daily gain is improved, reduces feedstuff-meat ratio, reduction is produced into
This.
Specific embodiment
The present invention is described in further detail in conjunction with specific embodiments.
By hydrolysis, the product for being hydrolyzed to dipeptides or tripeptides is easy to absorb protein in human body than free amino acid,
Protein than not hydrolyzing more easily absorbs, therefore, when protein is hydrolyzed with different proteases, it is necessary to
Degree of being hydrolyzed measurement.
The definition of degree of hydrolysis: the degree of hydrolysis DH of protein represents protein in hydrolytic process, the degree that peptide bond is cleaved
Or percentage.
Its detection method is as follows: taking hydrolyzed protein liquid 5.00ml in small beaker, 60ml distilled water is added, then magnetic force stirs
It mixes and measures its pH value with Accurate pH meter, be then titrated to pH=8.2 with 0.05mol/l NaOH solution, be then added and neutralized
Good formalin 10ml is then titrated to pH=9.2, records the 0.05mol/ by its pH value from post consumption when 8.2 titration 9.2
The volume of l NaOH solution, while reagent blank titration experiments (distilled water) is done, then calculate its degree of hydrolysis.The meter of degree of hydrolysis DH
It is as follows to calculate formula:
C is NaOH concentration, unit mol/l；
V1And V2The respectively volume of sample and the NaOH of blank consumption, is the volume that 9.2 are dropped to from PH8.2, unit is
ml；
V is the volume of protein enzymatic hydrolyzate, unit ml；
M is sample quality, unit g；
P is the mass percent of albumen in raw material, unit %；
8 be mM number mmol/g of peptide bond contained by every gram of protein (with reference to the value of soybean protein, if other albumen
Difference can be done and slightly adjust).
Embodiment 1
2000kg water is added in 650kg soybean protein, 250kg lard, 150kg soybean oil, 4kg emulsifier is then added,
Temperature controls between 50-60 DEG C, and stirring passes through homogenizer homogeneous micronization, pressure 25-35MPa or so, subsequently into mulser
It is handled, mulser rotor speed is 1500r/min or so, reasonable between quiet grinding, dynamic grinding, screw blade three
After forming the comprehensive effects such as strong, reciprocal aquashear, friction, centrifugation extruding, liquid stream collision in narrow gap, last
120 minutes or so, then by material in injection enzymatic vessel, pH value was 7.0 or so, and neutral proteinase 1.2kg, Papain is added
Enzyme 1.2kg, lipase 1.2kg, temperature control between 45-60 DEG C, at the uniform velocity stir 90 minutes, another reactor tank is then added
In, the zinc chloride of 0.5kg or so is added, stirs 40 minutes, is then spray-dried the spray-dried tower of material semi-finished product,
As powder, it is dry after product, be sieved, measure, packaging.Its degree of hydrolysis is 25.3%.
Embodiment 2
250kg soybean oil, 250kg palm oil, 450kg dregs of beans, 150kg plasma protein powder are placed in the aqueous solution of 2000kg
In, 8kg emulsifier is then added, temperature is controlled at 30-65 DEG C or so, and stirring is handled, mulser subsequently into mulser
Rotor speed is 16000r/min or so, is formed strongly, back and forth in the reasonable narrow gap of stator, rotor between the two
After the comprehensive effects such as aquashear, friction, centrifugation extruding, liquid stream collision, 50 minutes or so are lasted, material is added into enzymatic vessel
In, hay bacillus 1.5kg, papain 1.5kg, bromelain 1.0kg is added, temperature control is at 60 DEG C, pH value 7.0
Left and right at the uniform velocity stirs 90 minutes, is then added in another reactor tank, ferrous sulfate, the 0.1kg vitamin C of 0.5kg is added,
Stirring 30 minutes, then material semi-finished product are dried, become powder, the product after drying is sieved, measures, packaging.It is hydrolyzed
Degree is 28.7%.
Embodiment 3
220kg palm oil, 220kg soybean oil, 450kg dregs of beans, 200 kilograms of fish meal are placed in 2000kg aqueous solutions, so
18kg emulsifier is added afterwards, temperature controls the stirring at 45-56 DEG C or so, and into high pressure homogenizer homogeneous, pressure can be
It is freely adjusted in 380MPa, material is moved in circles the above course of work by circulation pipe in a reservoir, achieve the purpose that refinement,
It is handled subsequently into mulser, mulser rotor speed is 2800r/min, between stator, rotor, screw blade three
Reasonable narrow gap in form strong, reciprocal aquashear, friction, centrifugation squeeze, liquid stream collision etc. is after comprehensive effects,
70 minutes or so are lasted, then material is added in enzymatic vessel, pH value 5.5-7.5, bromelain 3.5kg, fibre is added
Plain enzyme 1.0kg is tieed up, temperature controls between 40-60 DEG C, digests 160 minutes, is then injected into reactor tank, and 0.8kg protochloride is added
Iron is stirred for 30 minutes, and the spray-dried tower of semi-finished product is spray-dried.As powder, it is dry after product, be sieved, meter
Amount, packaging.Its degree of hydrolysis is 31.7%.
Embodiment 4
By 150kg whey powder, 220kg soybean protein, 220kg rice protein, 200kg soybean oil, 250kg palm oil, set
Enter in the 2000kg aqueous solution that pH value is 6.0-7.5,15kg emulsifier is then added, temperature is controlled at 45 DEG C or so, is added
1.2kg zinc sulfate, 0.5kg vitamin E, stirring are handled subsequently into mulser, and mulser rotor speed is 4800r/
Min forms strong, reciprocal aquashear in the reasonable narrow gap between stator, rotor, screw blade three, rubs
It wipes, the comprehensive effects such as centrifugation squeezes, liquid stream collision, after lasting 90 minutes or so, is added to enzymatic vessel, food flavor enzyme 1kg, withered is added
Straw bacterium 1.5kg, bromelain 2.5kg, pH value 6-7.5, temperature control between 35 DEG C -65 DEG C, digest 64 hours, and half
The spray-dried tower of finished product is spray-dried.As powder, it is dry after product, be sieved, measure, packaging.Its degree of hydrolysis is
37.7%.
Embodiment 5
200kg soybean oil, 200kg palm oil, 350kg dregs of beans, 150kg plasma protein are placed in 2000kg aqueous solution,
Emulsifier is added, temperature is controlled at 35-60 DEG C or so, handled subsequently into mulser, mulser rotor speed is
2700r/min or so forms strong, reciprocal liquid in the reasonable narrow gap between stator, rotor, screw blade three
After the comprehensive effects such as power shearing, friction, centrifugation extruding, liquid stream collision, 60 minutes or so are lasted, material is injected in enzymatic vessel,
Hay bacillus 1.5kg, bromelain 2.5kg is added, temperature controls between 35-60 DEG C, and pH value is even between 6.5-7.5
Speed stirring 300 minutes after, be added 0.4kg manganese sulfate, 0.3kg vitamin B1, stir 30 minutes, the spray-dried tower of semi-finished product into
Row spray drying.As powder, it is dry after product, be sieved, measure, packaging.Its degree of hydrolysis is 25.5%.
Embodiment 6
700kg soybean protein, 300kg fish meal being placed in 1000kg aqueous solution, temperature controls between 35 DEG C -50 DEG C,
Into high pressure homogenizer homogeneous, pressure can freely adjust in 400MPa, be recycled material in a reservoir by circulation pipe past
The multiple above course of work, achievees the purpose that refinement, and then material is added in enzymatic vessel, and alkali protease 2.5kg, pawpaw is added
Protease 2.0kg, pH value 6.0-9.5, temperature control at 35-60 DEG C or so, at the uniform velocity stir 300 minutes, be subsequently poured into another
In reactor tank, 0.8kg copper sulphate, 0.3kg vitamin D is added, stirs 40 minutes, semi-finished product drying machine drying, after dry
Product, crush, sieving, measure, packaging.Its degree of hydrolysis is 21.7%.
Embodiment 7
250kg soybean oil, 250kg palm oil, 350kg dregs of beans, 150kg plasma protein are placed in 2000kg water, temperature control
System at 40-60 DEG C or so, then be added 10kg emulsifier, ferrous sulfate, the 5kg vitamin C of 0.5kg is added, stir, then into
Enter mulser to be handled, mulser rotor speed is 18000r/min or so, in quiet grinding, dynamic grinding between the two reasonable
After forming the comprehensive effects such as strong, reciprocal aquashear, friction, centrifugation extruding, liquid stream collision in narrow gap, 80 are lasted
Minute or so, material is added in enzymatic vessel, hay bacillus 1.5kg is added, papain 1.5kg, pH value is in 6.5-7.5
Between, temperature control at 60 DEG C, at the uniform velocity stirring enzymatic hydrolysis 360 minutes, the spray-dried tower of material semi-finished product is spray-dried,
As powder, it is dry after product, be sieved, measure, packaging.Its degree of hydrolysis is 28.7%.
Embodiment 8
50kg whey powder, 500kg rice protein, 250kg palm oil, 150kg soybean oil, 50kg intestine membrane protein are added
2000kg water, temperature control between 50-60 DEG C, and 5kg emulsifier is added, and 0.2kg sodium selenite, 0.2kg vitamin E is added,
Stirring, by homogenizer homogeneous micronization, pressure material moved in circles in a reservoir in 15-25MPa or so, by circulation pipe with
The upper course of work, adds and is handled subsequently into mulser, and mulser rotor speed is 2900r/min or so, stator,
Strong, reciprocal aquashear, friction are formed in reasonable narrow gap between rotor sheet, screw blade three, are centrifuged and are squeezed
After the comprehensive effects such as pressure, liquid stream collision, 90 minutes or so are lasted, then pours into material in enzymatic vessel, neutral proteinase is added
1.5kg, papain 1.5kg, pH value is between 6.5-7.5, temperature controls between 50-60 DEG C, stirring 300 minutes, so
The spray-dried tower of material semi-finished product is spray-dried afterwards, becomes powder, the product after drying is sieved, measures, packaging.
Its degree of hydrolysis is 26.3%.
Embodiment 9
50kg whey powder, 150kg coconut oil, 300kg palm oil, 500kg wheat gluten are placed in the aqueous solution of 2000kg
In, temperature is controlled at 50 DEG C or so, and 10kg emulsifier is then added, and ferrous sulfate 0.3kg, cobalt chloride 0.1kg, sulphur is then added
Sour copper 0.2kg, manganese sulfate 0.05kg, stirring, are handled into mulser, and mulser rotor speed is 2900r/min, left
The right side forms strong, reciprocal aquashear in the reasonable narrow gap between stator, rotor, friction, centrifugation squeeze, liquid
After the comprehensive effects such as stream collision, it is then injected into homogenizer, by homogenizer homogeneous micronization, pressure passes through circulation in 80MPa or so
Pipe material the moves in circles in a reservoir above course of work pours into material in enzymatic vessel after so lasting 60 minutes or so, adds
Enter hay bacillus 1.5kg, papain 1.5kg, bromelain 1.0kg, pH value is between 6.5-7.5, temperature control exists
It 50 DEG C or so, at the uniform velocity stirs 180 minutes, then the spray-dried tower of material semi-finished product is spray-dried again, becomes powder,
Product after drying is sieved, and measures, packaging.Its degree of hydrolysis is 25.2%.
Embodiment 10
250kg soybean oil, 250kg palm oil, 350kg dregs of beans, 150kg fish meal are placed in 2000kg water, and temperature control exists
20-60 DEG C or so, 10kg emulsifier is then added, potassium iodide, the 0.3kg vitamin K of 0.5kg is added, stirs, subsequently into cream
Change machine is handled, and mulser rotor speed is 18000r/min or so, stator, rotor between the two it is reasonable it is narrow between
After forming the comprehensive effects such as strong, reciprocal aquashear, friction, centrifugation extruding, liquid stream collision in gap, 80 minutes left sides are lasted
The right side adds material in enzymatic vessel, be added hay bacillus 1.5kg, papain 1.5kg, 1.5 kilograms of bromelain,
PH value is between 6.5-8.5, temperature is controlled at 60 DEG C, at the uniform velocity stirring enzymatic hydrolysis 360 minutes, by the spray-dried tower of material semi-finished product
It is spray-dried, becomes powder, the product after drying is sieved, measures, packaging.Its degree of hydrolysis is 27.6%.
Embodiment 11
700kg soybean protein, 300kg fish meal being placed in 1000kg aqueous solution, temperature controls between 35 DEG C -50 DEG C,
Into colloid mill, formed in stator (quiet grinding), the reasonable narrow gap of rotor (dynamic grinding) between the two strong, reciprocal
Aquashear, friction, centrifugation squeeze, liquid stream collision etc. is after comprehensive effects, material recycled in a reservoir by circulation pipe past
The multiple above course of work, achievees the purpose that refinement, and then material is added in enzymatic vessel, and alkali protease 2.5kg, pawpaw is added
Protease 2.0kg, pH value 6.0-9.5, temperature control at 35-60 DEG C or so, at the uniform velocity stir 300 minutes, be subsequently poured into another
In reactor tank, 0.8kg zinc sulfate, 0.3kg vitamin E is added, 0.3kg ferrous sulfate, 6kg vitamin C stir 40 minutes,
The drying of semi-finished product drying machine, the product after drying are crushed, sieving, are measured, packaging.Its degree of hydrolysis is 21.7%.
Above-mentioned specific embodiment is used to illustrate the present invention, is merely a preferred embodiment of the present invention, rather than
Limit the invention, within the spirit of the invention and the scope of protection of the claims, to the present invention make any modification,
Equivalent replacement, improvement etc., both fall within protection scope of the present invention.
Claims (11)
1. a kind of preparation method with enzymatic hydrolysis small peptide-additive complex compound feed, which is characterized in that described to have enzymatic hydrolysis small
The preparation method of peptide-additive complex compound feed in turn includes the following steps:
(1) it adds raw materials into water, emulsifier is added, then heating is added additive, is sufficiently stirred；The additive includes
The compound of co-ordinate covalent bond can be formed with amino；
(2) mixture obtained in step (1) is added in homogenizer and carries out homogenization；Or it will be obtained in step (1)
Mixture be added in mulser and carry out emulsification treatment；Or mixture obtained in step (1) is added in homogenizer
Homogenization is carried out, then the mixture after homogenization is added in mulser and carries out emulsification treatment；Or by step (1)
Obtained in mixture be added in mulser and carry out emulsification treatment, the mixture after emulsification treatment is then added to homogenizer
Middle carry out homogenization；Or according to raw material and the mixed viscosity situation of water, homogenizer is replaced with into colloid mill, or by glue
Body grinds the preceding road that homogenizer is arranged in；
(3) semi-finished product obtained in step (2) are added to enzymatic vessel, enzyme are added, is digested under certain temperature and pH value,
In the range of 20 ~ 100 DEG C, pH is controlled in the range of 1.5 ~ 12.0 heating and temperature control；
(4) by the semi-finished product that step (3) obtains be dried to powder to get to it is a kind of with enzymatic hydrolysis small peptide-additive complex compound
Feed；
The raw material include: mass percent be 0 ~ 60% whey powder, 0 ~ 80% vegetable protein, 0 ~ 80% animal protein, 0 ~
80% vegetable fat, 0 ~ 80% animal fat carry out mixing in proportion and be made into raw material, and at least there is vegetable protein or animal
One of albumen.
2. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 1, feature exist
In the enzyme includes: at least one protease and/or carbohydrase, lipase, food flavor enzyme, cellulase, Bacillus acidi lactici, double qi bars
One of bacterium, hay bacillus, redox enzymes, isomerase, enzyme inhibitor, zymoexciter or a variety of combinations.
3. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 1, feature exist
In, the additive include: soluble coordinating metal salt, the solubility coordinating metal salt include: copper, ferrous iron, manganese, zinc,
At least one of iodine, cobalt, molybdenum, selenium, nickel, vanadium, chromium, soluble metallic salt of lithium.
4. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 3, feature exist
In the additive further include: at least one of vitamin, microelement, acidulant and flavouring agent.
5. a kind of system described in any one of -4 according to claim 1 with enzymatic hydrolysis small peptide-additive complex compound feed
Preparation Method is preparation-obtained to have enzymatic hydrolysis small peptide-additive complex compound feed.
6. a kind of preparation method with enzymatic hydrolysis small peptide-additive complex compound feed, which is characterized in that described to have enzymatic hydrolysis small
The preparation method of peptide-additive complex compound feed includes:
(1) it adds raw materials into water, heat and is sufficiently stirred；
(2) mixture obtained in step (1) is added in homogenizer and carries out homogenization；Or it will be obtained in step (1)
Mixture be added in mulser and handled；Or mixture obtained in step (1) is added in homogenizer and is carried out
Then mixture after homogenization is added in mulser and handles by homogenization；Or it will be obtained in step (1)
Mixture is added in mulser and is handled, and then the mixture after emulsification treatment is added in homogenizer and is carried out at homogeneous
Reason；Or according to raw material and the mixed viscosity situation of water, homogenizer is replaced with into colloid mill, or colloid mill is arranged equal
The road Zhi Jiqian；
(3) semi-finished product obtained in step (2) are added to enzymatic vessel, enzyme is added, carries out enzyme at certain temperature and pH value
Solution；In the range of 20 ~ 100 DEG C, pH is controlled in the range of 1.5 ~ 12.0 heating and temperature control；
(4) semi-finished product that step (3) obtains are added in another reactor tank, additive, stirring a period of time is then added；Institute
Stating additive includes the compound that co-ordinate covalent bond can be formed with amino；
(5) by the semi-finished product that step (4) obtains be dried to powder to get to it is a kind of with enzymatic hydrolysis small peptide-additive complex compound
Feed；
The raw material include: mass percent be 0 ~ 60% whey powder, 0 ~ 80% vegetable protein, 0 ~ 80% animal protein, 0 ~
80% vegetable fat, 0 ~ 80% animal fat carry out mixing in proportion and be made into raw material, and at least there is vegetable protein or animal
One of albumen.
7. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 6, feature exist
In the enzyme includes: at least one protease and/or carbohydrase, lipase, food flavor enzyme, cellulase, Bacillus acidi lactici, double qi bars
One of bacterium, hay bacillus, redox enzymes, isomerase, enzyme inhibitor, zymoexciter or a variety of combinations.
8. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 6, feature exist
In, the additive include: soluble coordinating metal salt, the solubility coordinating metal salt include: copper, ferrous iron, manganese, zinc,
At least one of iodine, cobalt, molybdenum, selenium, nickel, vanadium, chromium, soluble metallic salt of lithium.
9. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 8, feature exist
In the additive further include: at least one of vitamin, microelement, acidulant and flavouring agent.
10. the preparation method with enzymatic hydrolysis small peptide-additive complex compound feed according to claim 6, feature exist
In, can also include: to add raw materials into water in the step (1), be added emulsifier, then heat and be sufficiently stirred.
11. a kind of have enzymatic hydrolysis small peptide-additive complex compound feed according to any one of claim 6-10
Preparation method is preparation-obtained to have enzymatic hydrolysis small peptide-additive complex compound feed.
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|---|---|---|---|---|
| CN103766609A (en) * | 2014-02-13 | 2014-05-07 | 哈尔滨伟平科技开发有限公司 | Preparation method of polypeptide feed additive |
| CN105211636A (en) * | 2015-08-25 | 2016-01-06 | 杭州香保饲料有限公司 | A kind of hybrid process method of grease, albumen, whey powder, feed addictive |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN106480154A (en) * | 2016-12-30 | 2017-03-08 | 亚太星原农牧科技海安有限公司 | A kind of small-peptide chelated zinc and preparation method and application |
| CN106721283A (en) * | 2016-12-09 | 2017-05-31 | 广州傲农生物科技有限公司 | Small-peptide chelated manganese compound of one main laver and preparation method and application |
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2018
- 2018-01-31 CN CN201810093476.2A patent/CN110089634A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766609A (en) * | 2014-02-13 | 2014-05-07 | 哈尔滨伟平科技开发有限公司 | Preparation method of polypeptide feed additive |
| CN105211636A (en) * | 2015-08-25 | 2016-01-06 | 杭州香保饲料有限公司 | A kind of hybrid process method of grease, albumen, whey powder, feed addictive |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN106721283A (en) * | 2016-12-09 | 2017-05-31 | 广州傲农生物科技有限公司 | Small-peptide chelated manganese compound of one main laver and preparation method and application |
| CN106480154A (en) * | 2016-12-30 | 2017-03-08 | 亚太星原农牧科技海安有限公司 | A kind of small-peptide chelated zinc and preparation method and application |
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