CN110089357A - A kind of cuttage and seedling culture method of radix tetrastigme - Google Patents
A kind of cuttage and seedling culture method of radix tetrastigme Download PDFInfo
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- CN110089357A CN110089357A CN201910392616.0A CN201910392616A CN110089357A CN 110089357 A CN110089357 A CN 110089357A CN 201910392616 A CN201910392616 A CN 201910392616A CN 110089357 A CN110089357 A CN 110089357A
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- radix tetrastigme
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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Abstract
The present invention provides a kind of cuttage and seedling culture method of radix tetrastigme; it is related to conservation of plant resources and utilizes technical field; the cuttage and seedling culture method of radix tetrastigme of the present invention; using two semi-lignified, band base of leaf sections as cuttings; after cuttings is stained with hormotone solution of the leaching containing IBA and NAA; it is inserted into the cutting medium being contained in non-woven bag, cutting medium includes red soil, rice chaff ash and calcium superphosphate；The temperature and light transmittance when culture are controlled after cuttage, obtain radix tetrastigme rooted seedling；Radix tetrastigme rooted seedling, cutting medium and non-woven bag are integrally transplanted.Cuttage and seedling culture method of the present invention is suitable for that indoor industrially produces radix tetrastigme seedling, and the rooting rate of cuttage reaches 77% or more, according to the high survival rate after radix tetrastigme seedling replanting 6 months of the method for the invention cultivation in 67%.
Description
Technical field
The present invention relates to conservation of plant resources and utilize technical field more particularly to a kind of cuttage and seedling culture side of radix tetrastigme
Method.
Background technique
Radix tetrastigme, that is, tetratigma hemsleyanum is Vitaceae Tetrastigma perennial evergreen liana, is the distinctive treasure in China
Dilute medicinal plant.Radix tetrastigme has high medical value, economic value and ornamental value.Complete stool can be used as medicine, with 3 years with
On root tuber drug effect it is best.Its root tuber is known as hemsley rockvine root, is civil common Chinese herbal medicine, has clearing heat and detoxicating, promoting blood circulation by removing wind
Effect.For treating hyperpyretic convulsion, pneumonia, asthma, hepatitis, rheumatism, irregular menstruation, pharyngalgia, scrofula, carbuncle furunculosis furuncle and pernicious swollen
Tumor has a good effect, and without any side effects.
The extract of radix tetrastigme has been used to the clinical treatment of a variety of diseases at present.Radix tetrastigme is evergreen all the year round, shade tolerant, resistance to
Cold, winter hangs up red festoons fruit long, and emerald green palmately compound leaf, is gardens vertical greening, indoor pot good material in addition.Radix tetrastigme happiness is cool
Cool and refreshing air time, thermophilic are frequently grown in hillside shrubbery, mountain valley, small stream side hayashishita rock seam, height above sea level 300- in 25 DEG C or so robust growths
1300m, because of place of production difference, shape, pharmacological property can slightly have difference.Radix tetrastigme seed is since progenesis is immature and exosper
It influences, there is certain influence on the germination of radix tetrastigme seed.It is wanted due to its unique reproductive characteristic and to the special of growing environment
It asks, radix tetrastigme wild resource is rare, however in recent years due to the digging of spreading unchecked of people, wild resource is more rare, therefore realizes
Artificial breeding cultivation, the scale, industrialized development of radix tetrastigme all have weight to medical industry, human health, horticulture industry etc.
Big meaning.
For radix tetrastigme there is presently no the formal kind by authorization, the kind of production and application is farm variety, academia
With civil from leaf blade size and shape, surface keratin degree, radix tetrastigme is angularly divided into purple by limb sawtooth, rattan color and shape
Rattan radix tetrastigme and sinomenium acutum radix tetrastigme, it is considered that sinomenium acutum radix tetrastigme root tuber is cold in nature, and medicinal efficacy is not so good as Chinese wistaria radix tetrastigme, dosage
There may be certain security risk greatly, therefore Chinese wistaria radix tetrastigme has higher medical value and economic value, have broader
Application prospect.The purple flat rattan radix tetrastigme of leaflet is derived from Wuyi Mountain north slope height above sea level 300~800m small stream side underbrush, is Chinese wistaria three
One kind of Ye Qing, it is resistant it is strong, growth is fast, superior quality plurality of advantages, there is high application value.
The method of artificial culture radix tetrastigme seedling has the methods of cuttage, sowing, Plant Tissue Breeding.Radix tetrastigme plant tissue
Culture technique comparative maturity, but it is at high cost, many instrument and equipments are needed, operating technology is inconvenient, and seed propagation has not been reported,
But cutting propagation is at low cost, the facilities and equipment needed is few, and breeding operating technology is simple and fast.Cutting propagation research starting is compared
In evening, relevant document report is seldom, it is auspicious brave equal research shows that after 1000mg/L IBA dipping 2~triennial old branch 10s cuttage exist
Rooting efficiency is best in loess.Hua Jinwei carries out cuttage examination by using 6-BA+IAA and root-growing agent the processing cuttings of various concentration
It tests, obtains the rooting percent handled with 6-BA+IAA (mass concentration ratio 2:1) 2500mg/kg for 46%.Cutting propagation at present
Though rooting rate is not high it has been reported that still technology is still immature, it is difficult to meet the needs of large-scale planting.
Summary of the invention
The present invention provides a kind of three leaves for the defect for overcoming the rooting rate of prior three-blade blueness cutting propagation not high
Green cuttage and seedling culture method, cutting plantation is high, and transplanting survival rate is high.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of cuttage and seedling culture methods of radix tetrastigme, comprising the following steps:
(1) the two base of leaf sections of semi-lignified, band for intercepting radix tetrastigme, obtain radix tetrastigme cuttings；
(2) after the morphology lower end of radix tetrastigme cuttings to be stained with to the hormotone solution of leaching IBA and NAA, insertion is contained in nothing
Cutting medium in woven fabric bag；
(3) radix tetrastigme after cultivating cuttage obtains radix tetrastigme rooted seedling to taking root；In 35~45d after cuttage, control ring
Border temperature is in 25~30 DEG C, light transmittance 40~50%；
(4) radix tetrastigme rooted seedling is integrally transplanted together with non-woven bag and cutting medium therein.
Preferably, in the step (1), the semi-lignified of the radix tetrastigme, two base of leaf sections of band length be 10~
12cm, there are two internodes, and two leaves are had on second section, and two leaves are palmately compound leaf.
Preferably, in the step (2), the IBA concentration in hormotone solution is 0.05~0.3mg/g；Hormotone
NAA concentration in solution is 0.05~0.3mg/g.
Preferably, in the step (2), be stained with soak the hormotone solution time be 10~15min.
Preferably, in the step (2), cutting medium includes red soil, rice chaff ash and calcium superphosphate.
Preferably, in the step (2), the volume ratio of red soil, rice chaff ash and calcium superphosphate is 60~70:25~40:1
~5.
Preferably, in the step (3), environment temperature is controlled in 25~30 DEG C, method packet of the light transmittance 40~50%
It includes: building Small plastic shed above the radix tetrastigme scion after cuttage, the Small plastic shed includes backing material and plastic foil.
Preferably, primary every 6~8d watering in the step (3).
Preferably, it in the step (4), is sprinkled profoundly water immediately after transplanting.
Preferably, the kind of the radix tetrastigme includes the purple flat rattan radix tetrastigme of leaflet.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of cuttage and seedling culture methods of radix tetrastigme, using two semi-lignified, band base of leaf sections as slotting
Fringe is inserted into the cutting medium being contained in non-woven bag, cuttage base after cuttings is stained with hormotone solution of the leaching containing IBA and NAA
Matter includes red soil, rice chaff ash and calcium superphosphate；The temperature and light transmittance when culture are controlled after cuttage, are obtained radix tetrastigme and are taken root
Seedling；Radix tetrastigme rooted seedling, cutting medium and non-woven bag are integrally transplanted.Cuttage and seedling culture method of the present invention is suitable
Suitable indoor industrially produces radix tetrastigme seedling, and the rooting rate of cuttage reaches 77% or more, cultivates according to the method for the invention
High survival rate of the radix tetrastigme seedling replanting after 6 months is in 67%.
Specific embodiment
The present invention provides a kind of cuttage and seedling culture methods of radix tetrastigme, comprising the following steps:
(1) the two base of leaf sections of semi-lignified, band for intercepting radix tetrastigme, obtain radix tetrastigme cuttings；
(2) after the morphology lower end of radix tetrastigme cuttings to be stained with to the hormotone solution of leaching IBA and NAA, insertion is contained in nothing
Cutting medium in woven fabric bag；
(3) radix tetrastigme after cultivating cuttage obtains radix tetrastigme rooted seedling to taking root；In 35~45d after cuttage, control ring
Border temperature is in 25~30 DEG C, light transmittance 40~50%；
(4) radix tetrastigme rooted seedling is integrally transplanted together with non-woven bag and cutting medium therein.
The method of the invention can be suitable for industrialized seedling raising manners, easy to operate, easy to spread.
The present invention intercepts two base of leaf sections of semi-lignified, band of radix tetrastigme, obtains radix tetrastigme cuttings.It is currently preferred to adopt
With old branch cuttage, specifically, the preparation of radix tetrastigme cuttings of the present invention the following steps are included:
The radix tetrastigme plant of A1, selection no disease and pests harm, robust growth, its base portion branch of clip；The base portion branch it is close
It is semi-lignified at base portion；
A2, on the basis of retaining two of base portion branch sections, retaining two leaf palmately compound leaves on second section, according to
10~12cm of length trims base portion branch, obtains radix tetrastigme cuttings.
In the present invention, it preferably sprinkles water in the preparation process of the radix tetrastigme cuttings, to keep the humidity of cuttings.
The present invention selects the radix tetrastigme branch of two semi-lignified, band bases of leaf as scion, is on the one hand because semi-lignified material is rich
Richness carries out the nursery of batch production throughout the year；It is because the branch of semi-lignified is not allowed compared with edible tender branch on the other hand
Easy dehydration is wilted, and photosynthesis appropriate can be kept while reducing rising dehydration by retaining a small amount of blade.
After obtaining radix tetrastigme cuttings, the hormotone of leaching IBA and NAA is stained in the morphology lower end of radix tetrastigme cuttings by the present invention
After solution, it is inserted into the cutting medium being contained in non-woven bag.
In the present invention, in the hormotone solution, the concentration of IBA is preferably 0.05~0.3mg/g, more preferably
0.1mg/g；The concentration of NAA is preferably 0.05~0.3mg/g, more preferably 0.1mg/g.In the present invention, the hormotone
The solvent of solution is water.In the present invention, it is stained with the time that the hormotone solution is soaked with the morphology lower end of radix tetrastigme cuttings
Preferably 10~15min, more preferably 12~14min.In the present invention, callus can be promoted according to the hormone that the ratio mixes
The formation of tissue, the phase of taking root shift to an earlier date, and promote Gabor transform and the diameter increment of new root.
It in the present invention, preferably include red soil, rice chaff ash and calcium superphosphate in the cutting medium；The red soil
The volume ratio of soil, rice chaff ash and calcium superphosphate is preferably 60~70:25~40:1~5；More preferably 65:33:2.In the present invention
In, the red soil is commercially available to be bought or voluntarily prepares.If voluntarily preparing red soil can carry out as follows: taking forest land
The red soil on surface layer dries, and smashes it through 4cm and sieves to obtain the final product.When preparing cutting medium, according to volume ratio by red soil, rice chaff ash
It is stirred with calcium superphosphate.In the present invention, the red soil is played a supporting role as basic matrix, rice chaff
Ash plays the role of chesson, increases gas permeability, and calcium superphosphate provides nutrition, San Zhefu as slow-release fertilizer after new root is formed
It is capable of forming that a kind of nutrient is balanced, quality is loose and good cutting medium of breathing freely after matching.
In the present invention, the cutting medium preferably carries out disinfection before being packed into non-woven bag；More preferably using more
Bacterium spirit carries out disinfection.As shown in specific embodiments of the present invention, the cutting medium prepared is mixed with carbendazim dilute solution
Afterwards, with polybag it is closed for 24 hours more than.In the present invention, the non-woven bag preferably disappears before containing cutting medium
Poison.
In the present invention, insertion depth of the radix tetrastigme cuttings in cutting medium after being stained with leaching is preferably the 2/ of cutting medium
At 3 height.
After the completion of cuttage, the radix tetrastigme after culture cuttage of the present invention obtains radix tetrastigme rooted seedling to taking root；35 after cuttage
In~45d, environment temperature is controlled in 25~30 DEG C, light transmittance 40~50%.35~45d of the present invention after cuttage adjusts skewer
Ambient humidity, temperature and the illumination of radix tetrastigme scion after inserting are provided with the condition for being formed and being grown conducive to root restriction, promote to insert
Fringe Rapid Rooting.
In the present invention, control environment temperature 25~30 DEG C, light transmittance 40~50% method preferably include:
Small plastic shed is built above radix tetrastigme scion after cuttage, the Small plastic shed includes backing material and plastic foil.The backing material
The including but not limited to material that can be used as support such as timber, steel.In the present invention, in order to which light transmittance is on the basis of plastic foil
Also settable sunshade net is cooperated.In the present invention, in order to maintain the also settable blower of the environment temperature in Small plastic shed and water
Curtain.It is currently preferred, it is primary every 6~8d watering.The present invention passes through control watering time regulating environmental humidity.
In the present invention, the situation of taking root that cuttings is observed when 35~45d after cuttage, is opened simultaneously after cuttage rooting is good
Remove Small plastic shed and other corresponding facilities.It is currently preferred, after removing Small plastic shed, sprays every 3~5d and once contain
The water solubility fertilizer of humic acid.The Water soluble fertilizer containing humic acid provides full nutrition for rooted seedling, plays the role of strong sprout.At this
It is described when spraying in invention, it will be sprayed again after the water solubility fertilizer dilution 400~500 containing humic acid.
After obtaining radix tetrastigme rooted seedling, the present invention is whole together with non-woven bag and cutting medium therein by radix tetrastigme rooted seedling
Body transplanting.Rooted seedling is capable of the integrality of effective protection root system together with the mode that non-woven bag is integrally transplanted.Non-woven bag has
It is the characteristics of fast degradation, i.e. degradable in 90d after whole transplanting, the growth of root system, thus nursery side of the present invention are not influenced
Method can significantly improve the transplanting survival rate of radix tetrastigme seedling, the basic round-the-clock cultivation for realizing radix tetrastigme seedling.Applied to work
It can accomplish batch-order production, the supply of healthy and strong seedling in the production of industry metaplasia.
In the present invention, it is carried out after 100~120d of the transplanting of radix tetrastigme rooted seedling usually after cuttage.It, will when transplanting
The radix tetrastigme takes root transplantation of seedlings into field or culture apparatus, sprinkles profoundly water immediately after transplanting.In the present invention, after transplanting
The light transmittance of radix tetrastigme rooted seedling need to be 50% hereinafter, being more advantageous to surviving for radix tetrastigme rooted seedling.
In the present invention, there are serious continuous cropping obstacles for radix tetrastigme, can not continuously transplant in same forest land, the present invention
In order to overcome the problems, such as that this preferably integrally transplants radix tetrastigme rooted seedling into culture apparatus, the culture apparatus is arranged in field
Between.After radix tetrastigme harvest after transplanting, new soil or cultivation matrix are inserted into culture apparatus, same forest land can be realized
Lower continuous transplanting radix tetrastigme, overcomes radix tetrastigme Soil-sickness Problem.In the present invention, the culture apparatus includes but is not limited to spend
Basin or cultivating bag.
The cuttage and seedling culture method of radix tetrastigme of the present invention is also applied for the cuttage and seedling culture of the purple flat rattan radix tetrastigme of leaflet.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Cutting medium prepares: 1) taking Forest floor red soil and dry, crush, is spare after mistake 4cm sieve；2) according to red soil
Soil: rice chaff ash: red soil, rice chaff ash and calcium superphosphate are sufficiently stirred calcium superphosphate=65:33:2 volume ratio, so that each
Ingredient is uniformly mixed, and forms cutting medium；3) 12.5% synergy carbendazim soluble concentrate is watered and is diluted to 300 times of solution, led to
It crosses the method sprayed when digging to mix well carbendazim dilution with cutting medium, be covered after piling conical pile with plastic foil
It is sealed after covering or being directly loadable into polybag, it is sealing 24 hours or more, spare after sterilizing completely.
Cuttings prepares: 1) from three leaf of base portion clip on the purple flat rattan radix tetrastigme plant of leaflet of no disease and pests harm, robust growth
Green branch chooses the branch of the semi-lignified close to base portion, is 10cm according to length, and the standard clip for retaining two internodes is inserted
Fringe, and retain two leaf palmately compound leaves on each cuttings two sections, i.e. two base of leaf sections of semi-lignified band, in cuttings clip mistake
Cheng Zhong keeps cuttings humidity using watering；2) by the cuttings after trimming according to the standard of 50 every bundles arrange it is neat after be bundled into
Bundle, morphology lower end, that is, cuttage end is soaked in IBA (100ppm) and NAA (100ppm) hormotone solution 10 minutes, is taken
Prepare cuttage out.
Cuttage and management: 1) it by the cutting medium of sterilization treatment dispenses to the non-woven bag of 8cm × 12cm, in greenhouse
It is put neatly in greenhouse according to 15 × 200 square matrixes；2) cuttings of dipping HORMONE TREATMENT is inserted into non-woven bag center position
Then matrix is sprinkled profoundly water with being advisable in 2/3 insertion matrix；3) Small plastic shed in canopy is built using bamboo stick and plastic foil, supplemented weekly
The suitable humidity of water tariff collection, blower, cascade and inside and outside sunshade net by adjusting greenhouse maintain temperature at 27 DEG C, light transmission
Rate is between 40%~50%；4) 35~45 days or so observation cuttage rooting situations are opened and are removed after cuttage rooting is good
Sunshade net, plastic foil and bamboo stick spray 400 times of dilutions of primary water-soluble fertilizer containing humic acid for every 3 days.
Transplanting: it is integrally transplanted using rooted seedling, matrix and non-woven bag, 1) transplanting process specifically included that after 100-120 days
Select the seedling of robust growth together with non-woven bag, heatable adobe sleeping platform to bamboo grove, and is filled consolidation with soil, prevents from accumulating by transplanting
Water；2) it after transplanting, sprinkles profoundly water immediately；3) maintain light transmittance below 50%.
Situation of taking root in Statistics Implementation example 1 after the purple flat rattan radix tetrastigme cuttage of leaflet, the results are shown in Table 1:
The cuttage root-taking result of the purple flat rattan radix tetrastigme of 1 leaflet of table
| Average germination rate | Rooting rate | Mean elements | Average root long | Young sprout average length |
| 87.23% | 77.34% | 14.36 | 4.53cm | 6.45cm |
Embodiment 2
Cutting medium prepares: 1) taking Forest floor red soil and dry, crush, is spare after mistake 4cm sieve；2) according to red soil
Soil: rice chaff ash: red soil, rice chaff ash and calcium superphosphate are sufficiently stirred calcium superphosphate=60:30:3 volume ratio, so that each
Ingredient is uniformly mixed, and forms cutting medium；3) 12.5% synergy carbendazim soluble concentrate is watered and is diluted to 300 times of solution, led to
It crosses the method sprayed when digging to mix well carbendazim dilution with cutting medium, be covered after piling conical pile with plastic foil
It is sealed after covering or being directly loadable into polybag, it is sealing 24 hours or more, spare after sterilizing completely.
Cuttings prepares: 1) from three leaf of base portion clip on the purple flat rattan radix tetrastigme plant of leaflet of no disease and pests harm, robust growth
Green branch chooses the branch of the semi-lignified close to base portion, is 12cm according to length, and the standard clip for retaining two internodes is inserted
Fringe, and retain two leaf palmately compound leaves on each cuttings two sections, i.e. two base of leaf sections of semi-lignified band, in cuttings clip mistake
Cheng Zhong keeps cuttings humidity using watering；2) by the cuttings after trimming according to the standard of 50 every bundles arrange it is neat after be bundled into
Bundle, morphology lower end, that is, cuttage end is soaked in IBA (100ppm) and NAA (100ppm) hormotone solution 15 minutes, is taken
Prepare cuttage out.
Cuttage and management: 1) it by the cutting medium of sterilization treatment dispenses to the non-woven bag of 8cm × 12cm, in greenhouse
It is put neatly in greenhouse according to 15 × 200 square matrixes；2) cuttings of dipping HORMONE TREATMENT is inserted into non-woven bag center position
Then matrix is sprinkled profoundly water with being advisable in 2/3 insertion matrix；3) Small plastic shed in canopy is built using bamboo stick and plastic foil, supplemented weekly
The suitable humidity of water tariff collection, blower, cascade and inside and outside sunshade net by adjusting greenhouse maintain temperature at 27 DEG C, light transmission
Rate is between 40%~50%；4) 35~45 days or so observation cuttage rooting situations are opened and are removed after cuttage rooting is good
Sunshade net, plastic foil and bamboo stick spray 500 times of dilutions of primary water-soluble fertilizer containing humic acid for every 5 days.
Transplanting: it is integrally transplanted using rooted seedling, matrix and non-woven bag, 1) transplanting process specifically included that after 100-120 days
It selects the seedling of robust growth together with non-woven bag, transplants into cultivating bag, soil, the cultivation are housed in the cultivating bag
Bag is arranged in bamboo grove ground；2) it after transplanting, sprinkles profoundly water immediately；3) maintain light transmittance below 50%.
Comparative example 1
Other than individually transplanting radix tetrastigme rooted seedling to bamboo grove ground, other operations are same as Example 1.
6 months survival rates and growth shape after the purple flat rattan radix tetrastigme of leaflet is transplanted in Statistics Implementation example 1 and comparative example 1,2
Condition, the results are shown in Table 2:
Surviving after the taking root transplantation of seedlings 6 months of the purple flat rattan radix tetrastigme of table 2 leaflet and upgrowth situation
| Transplanting operation | Survival rate | Plant height | Ground fresh weight | Root system fresh weight | |
| Comparative example 1 | The direct transplanted bamboo forest land of rooted seedling | 55.31% | 23.36cm | 120.21g | 92.21g |
| Embodiment 1 | Whole transplanted bamboo forest land | 67.13% | 25.37cm | 156.32g | 108.25g |
| Embodiment 2 | Whole transplanting bamboo grove cultivating bag | 78.40% | 36.43cm | 178.23g | 110.20g |
As can be seen from Table 2, the transplanting survival rate of embodiment 1,2, plant height, ground fresh weight and root system fresh weight with comparison
Example 1 shows the survival rate after method provided by the invention can effectively improve radix tetrastigme transplanting, plant height, ground there are significant difference
Fresh weight and root system fresh weight.The present invention loads the cutting medium containing timbered soil using non-woven bag, integrally transplants after taking root
Radix tetrastigme seedling.A period of time after transplanting, non-woven bag is voluntarily degraded, so as to avoid the damage of root system, when without slow seedling
Between, greatly improve the transplanting survival rate of radix tetrastigme.
As can be seen from Table 2, embodiment 1,2 exists in terms of transplanting survival rate, plant height, ground fresh weight and root system fresh weight
Significant difference, this is because the transplanting destination that embodiment 1,2 uses is different.Since there is radix tetrastigme serious continuous cropping obstacle to ask
Radix tetrastigme, is directly transplanted the transplanting survival rate and growing way that can to bamboo grove reduce part radix tetrastigme rooted seedling by topic.The present invention is real
Applying example 2 is integrally transplanted radix tetrastigme rooted seedling to culture apparatus (i.e. cultivating bag), eliminates what continuous cropping grew radix tetrastigme
It may influence.I.e. the present invention using cultivating bag plant radix tetrastigme be it is a kind of it is novel can be in the side that same forest land is continuously managed
Formula.Using method provided by the invention, the filling object that need to only replace in culture apparatus can be transplanted again, will not influence three
It the growth of Ye Qing and survives.
In view of the foregoing it is apparent that the cutting plantation of radix tetrastigme cuttage and seedling culture method provided by the invention is high, transplanting at
Motility rate is high, and easy to operate, is easy to industrial applications.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of cuttage and seedling culture method of radix tetrastigme, comprising the following steps:
(1) the two base of leaf sections of semi-lignified, band for intercepting radix tetrastigme, obtain radix tetrastigme cuttings；
(2) after the morphology lower end of radix tetrastigme cuttings to be stained with to the hormotone solution of leaching IBA and NAA, insertion is contained in non-woven fabrics
Cutting medium in bag；
(3) radix tetrastigme after cultivating cuttage obtains radix tetrastigme rooted seedling to taking root；In 35~45d after cuttage, environment temperature is controlled
Degree is in 25~30 DEG C, light transmittance 40~50%；
(4) radix tetrastigme rooted seedling is integrally transplanted together with non-woven bag and cutting medium therein.
2. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (1), the half of the radix tetrastigme
Lignifying, the length of two base of leaf sections of band are 10~12cm, and there are two internodes, and have two leaves on second section, described
Two leaves are palmately compound leaf.
3. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (2), in hormotone solution
IBA concentration be 0.05~0.3mg/g；NAA concentration in hormotone solution is 0.05~0.3mg/g.
4. cuttage and seedling culture method according to claim 1 or 3, which is characterized in that in the step (2), it is described mixed to be stained with leaching
The time for closing hormone solution is 10~15min.
5. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (2), cutting medium includes red
Loam, rice chaff ash and calcium superphosphate.
6. cuttage and seedling culture method according to claim 5, which is characterized in that in the step (2), red soil, rice chaff ash
Volume ratio with calcium superphosphate is 60~70:25~40:1~5.
7. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (3), control environment temperature exists
25~30 DEG C, light transmittance in 40~50% method include: to build Small plastic shed above radix tetrastigme scion after cuttage, it is described small
Arched shed includes backing material and plastic foil.
8. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (3), water every 6~8d
Once.
9. cuttage and seedling culture method according to claim 1, which is characterized in that in the step (4), irrigated immediately after transplanting
Water.
10. cuttage and seedling culture method according to claim 1, which is characterized in that the kind of the radix tetrastigme includes leaflet purple
Flat rattan radix tetrastigme.
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