CN110036108A - 一种细菌β-1,3-葡聚糖酶及其编码基因与应用 - Google Patents

一种细菌β-1,3-葡聚糖酶及其编码基因与应用 Download PDF

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Publication number
CN110036108A
CN110036108A CN201680090696.9A CN201680090696A CN110036108A CN 110036108 A CN110036108 A CN 110036108A CN 201680090696 A CN201680090696 A CN 201680090696A CN 110036108 A CN110036108 A CN 110036108A
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sequence
protein
hydrolysis
oligosaccharides
portugal
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CN110036108B (zh
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江正强
秦臻
闫巧娟
游鑫
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China Agricultural University
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China Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本发明提供了一种β‑1,3‑葡聚糖酶及其编码基因与应用。所述β‑1,3‑葡聚糖酶是如下a)或b)或c)：a)氨基酸序列是序列表中序列2第29‑449位的蛋白质；b)在序列表中序列2第29‑449位所示的蛋白质的N端和/或C端连接标签得到的融合蛋白质；c)将序列表中序列2第29‑449位所示的氨基酸序列经过一个或几个氨基酸残基的取代和/或缺失和/或添加得到的且具有相同功能的蛋白质。还提供了所述β‑1,3‑葡聚糖酶在制备β‑1,3‑葡寡糖和/或高聚合度β‑1,3‑葡寡糖中的应用，以及一种生产高聚合度β‑1,3‑葡寡糖的方法。

Description

PCT国内申请，说明书已公开。

Claims (18)

  1. PCT国内申请，权利要求书已公开。
CN201680090696.9A 2016-11-09 2016-11-09 一种细菌β-1,3-葡聚糖酶及其编码基因与应用 Active CN110036108B (zh)

Applications Claiming Priority (1)

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PCT/CN2016/105187 WO2018086008A1 (zh) 2016-11-09 2016-11-09 一种细菌β-1,3-葡聚糖酶及其编码基因与应用

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CN110036108A true CN110036108A (zh) 2019-07-19
CN110036108B CN110036108B (zh) 2022-05-17

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WO (1) WO2018086008A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317636A (zh) * 2021-12-29 2022-04-12 苏州科宁多元醇有限公司 一种葡聚寡糖的制备方法
CN115927508A (zh) * 2022-08-29 2023-04-07 中国海洋大学 昆布多糖降解酶OUC-ScLam39在制备昆布寡糖中的应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020186030A1 (en) 2019-03-14 2020-09-17 The Procter & Gamble Company Cleaning compositions comprising enzymes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104745656A (zh) * 2015-03-20 2015-07-01 江南大学 一种利用热凝胶发酵液直接生产β-1,3-葡寡糖的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104745656A (zh) * 2015-03-20 2015-07-01 江南大学 一种利用热凝胶发酵液直接生产β-1,3-葡寡糖的方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SHRESTHA K.L.等: "Characterization and identification of essential residues of the glycoside hydrolase family 64 laminaripentaose-producing-β-1，3-glucanase.", 《PROTEIN ENG DES SEL》 *
无: "WP_016313499.1", 《GENBANK》 *
无: "WP_028540123.1", 《GENBANK》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317636A (zh) * 2021-12-29 2022-04-12 苏州科宁多元醇有限公司 一种葡聚寡糖的制备方法
CN115927508A (zh) * 2022-08-29 2023-04-07 中国海洋大学 昆布多糖降解酶OUC-ScLam39在制备昆布寡糖中的应用

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CN110036108B (zh) 2022-05-17

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