CN109937726B - Method for grafting tomato sleeve by utilizing stem section - Google Patents
Method for grafting tomato sleeve by utilizing stem section Download PDFInfo
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- CN109937726B CN109937726B CN201910121283.8A CN201910121283A CN109937726B CN 109937726 B CN109937726 B CN 109937726B CN 201910121283 A CN201910121283 A CN 201910121283A CN 109937726 B CN109937726 B CN 109937726B
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 26
- 240000003768 Solanum lycopersicum Species 0.000 title description 33
- 238000005520 cutting process Methods 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims abstract description 12
- 241000227653 Lycopersicon Species 0.000 claims abstract 10
- 206010052428 Wound Diseases 0.000 claims description 16
- 208000027418 Wounds and injury Diseases 0.000 claims description 16
- 238000009331 sowing Methods 0.000 claims description 15
- 239000013003 healing agent Substances 0.000 claims description 13
- 239000000758 substrate Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000012010 growth Effects 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000004745 nonwoven fabric Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 244000052616 bacterial pathogen Species 0.000 claims description 4
- 239000005747 Chlorothalonil Substances 0.000 claims description 3
- 208000035240 Disease Resistance Diseases 0.000 claims description 3
- 239000005822 Propiconazole Substances 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 125000004383 glucosinolate group Chemical group 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 230000007226 seed germination Effects 0.000 claims description 3
- 230000035040 seed growth Effects 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims description 3
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 229940023877 zeatin Drugs 0.000 claims description 3
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 2
- 239000004626 polylactic acid Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 5
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 238000013268 sustained release Methods 0.000 description 6
- 239000012730 sustained-release form Substances 0.000 description 6
- 230000035876 healing Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 229920001661 Chitosan Polymers 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000009335 monocropping Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000021243 milk fat Nutrition 0.000 description 1
- 229940051921 muramidase Drugs 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
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Abstract
A method for grafting a tomato sleeve by utilizing stem segments comprises the steps of respectively taking terminal buds and stem segments as scions according to the height and the number of leaves of scion seedlings, reserving 1-2 true leaves for each stem segment, taking 2-3 scions for each plant, firstly using a cutting blade at a position 6-7 CM away from a base position of a hypocotyl of a selected stock, obliquely cutting upwards at an angle of 35-45 degrees, enabling a cut surface to be flat and smooth, inserting a soft sleeve into the top of the cut surface of the stock, enabling the lower part of the cut surface of the stock to be located at the middle height position of the soft sleeve, selecting the scion seedlings with the same diameter as the stock from the selected scions, obliquely cutting downwards at an angle of 35-45 degrees at the hypocotyl position, inserting the cut surface of the cut scion seedlings into the soft sleeve downwards, enabling the cut surfaces of the stock and the scions to be tightly combined, and obtaining the tomato grafted seedlings. The method has the advantages that the stem segments of the terminal buds and the intercepted stems of the tomatoes are utilized for sleeve grafting, 2-3 scions can be formed on 1 seed, the seedling cost is reduced, the grafting speed is high, and the survival rate is high.
Description
Technical Field
The invention belongs to a vegetable grafting technology, and particularly relates to a method for grafting a tomato sleeve by utilizing a stem section.
Background
The tomato is one of the main vegetable varieties planted in south Zhejiang province in overwintering, the area of the tomato is over 5 ten thousand mu, the industry foundation is tall, due to the influence of factors such as continuous cropping obstacles, soil-borne diseases are serious, the tomato grafting becomes a main limiting factor influencing facility tomato cultivation, the grafting is a method for effectively relieving the continuous cropping obstacles, the tomato seed is applied in large area in production, the price of the tomato seed is very high at present, and the seed cost is too high due to the scion grafting technology of one tomato seedling and one tomato bud, so that the development of the industry is not facilitated. According to practical experience in recent years, a writer gropes a set of grafting and seedling raising technology by utilizing the tomato stem section sleeve, so that the tomato grafting speed is greatly improved, the survival rate of grafted seedlings is high, and the seedling raising cost is greatly reduced.
Disclosure of Invention
In order to solve the problems, the method for grafting the tomato sleeve by utilizing the stem segments has the advantages that the stem segment grafting is adopted, one scion can provide 2 stem segments besides one terminal bud, the utilization rate of plants can be greatly improved, and the seed cost is reduced.
In order to achieve the above purpose, the invention is solved by the following technical scheme: a method for grafting tomato sleeves by utilizing stem segments comprises the following steps:
the method comprises the following steps: selecting stock varieties, wherein the tomato stock is selected from Zhejiang stock I with developed root system, strong growth potential, strong disease resistance such as tomato bacterial wilt resistance and stress resistance and good scion affinity;
step two: selecting a scion variety, wherein the scion selection is Ouxiu 201 of a tomato variety which has good grafting affinity with stocks, is disease-resistant, high in yield, high in quality, adapts to local climatic conditions and grows infinitely;
step three: sowing and raising seedlings;
soaking the seeds in 55 deg.C warm water, maintaining the water temperature of 55 deg.C for 30min, stirring to heat the seeds uniformly to kill germs carried by the seeds;
preparing a substrate, wherein the substrate is required to meet the requirements of tomato seed germination and growth, is loose, breathable, water-retaining and fertilizer-retaining, does not have germs and meets the requirements of tomato seedling growth;
directly sowing the treated seeds in 50-hole plug trays filled with a matrix, sowing one seed in each hole, sowing the scion 3 days later than the stock, watering and covering a film after sowing, controlling the temperature of a seedbed at 25-30 ℃ in the daytime and 15-20 ℃ at night, removing the film in time after seedling emergence, enhancing the disease and insect control in the seedling stage, controlling the spindling of the seedlings by adopting a tide irrigation technology during the seedling cultivation, screening seedlings in the seedling cultivation management process, enabling the seedlings in the same tray to reach a state of uniform and neat size, and starting grafting when the stock and the scion seedlings grow 6-8 true leaves and 18-22 CM high;
step four: managing in a seedling stage;
the method comprises the steps of building a small arched shed after grafting for heat preservation and moisture preservation, covering thin non-woven fabrics according to weather conditions to avoid direct sunlight, requiring the temperature to be 25-28 ℃ in daytime and 18-20 ℃ at night, keeping the humidity to be 93-95% after grafting, when the temperature in the shed exceeds 30 ℃ and the relative humidity is more than 95%, removing a film to release air and remove moisture, gradually reducing the temperature and the humidity after 3 days, and reducing the temperature and the humidity to be 22-25 ℃ in daytime and 15-18 ℃ at night, keeping the humidity to be 75-80% after ventilation to reduce the humidity in the shed and avoid rotten seedlings, and also preventing overhigh humidity in a seedbed for a long time, removing the small arched shed and the non-woven fabrics after 10-12 days for normal seedling management, strengthening pest control, removing axillary buds of stocks in time, and removing non-survival grafted seedlings and disease-sensitive seedlings until the seedlings survive.
Further, in the first step, when 6-8 true leaves and 18-22 CM of plant height grow on the rootstock and the scion seedling, grafting is started, and the grafting comprises the following steps:
disinfecting shed rooms and seedlings 1 day before grafting, wherein the used medicaments are 400 times of 50% chlorothalonil wettable powder and 600 times of 66.5% propiconazole for sterilization and disinfection;
during grafting, terminal buds and stem sections can be respectively taken according to the height and the number of leaves of a scion seedling to be directly used as a scion, 1-2 true leaves are reserved on each stem section, 2-3 scions can be taken from each plant, a cutting blade is firstly used at the position 6-7 CM away from a base position of the hypocotyl of a selected stock and is upwards obliquely cut at an angle of 35-45 degrees, the cut surface is flat and smooth, a soft sleeve is inserted from the top of the cutting surface of the stock, the lower part of the cutting surface of the stock is positioned at the middle height position of the soft sleeve, the scion seedling with the same diameter as the stock is selected from the selected scions, the scion seedling is downwards obliquely cut at the lower hypocotyl position at an angle of 35-45 degrees, the cut cutting surface of the cut scion seedling is downwards inserted into the soft sleeve, and the two oblique cutting surfaces of the stock.
Furthermore, a grafting wound rapid healing agent is coated on the contact surface of the soft sleeve and the grafting position of the rootstock and the scion.
Further, the grafting wound rapid healing agent comprises 50-120 mu M of glucosinolate, 5-10 mu M of zeatin, 5-15 wt% of sucrose, 40mL of ethanol with the volume concentration of 95%, and the balance of water.
Furthermore, the inner wall of the soft sleeve is also provided with a slow release layer, and the grafting wound rapid healing agent is gradually released through the slow release layer.
Further, the soft sleeve is made of degradable materials.
Further, the degradable material is polylactic acid.
The invention has the advantages that: by adopting stem grafting, one scion can provide 2 stem segments besides one terminal bud, so that the utilization rate of plants can be greatly improved, and the seed cost is reduced. In addition, a soft sleeve is sleeved at the grafting wound, the junction of the scion and the stock is fixed, a slow release layer is arranged on the inner wall of the soft sleeve, and the rapid healing agent for the grafting wound is gradually released, so that the healing speed of the grafting wound is greatly improved.
Drawings
Fig. 1 is a schematic structural view of a soft sleeve used in the method for grafting the tomato sleeve by using the stem segment according to the present invention.
Fig. 2 is a schematic structural diagram of a soft sleeve and a slow release layer in the method for grafting the tomato sleeve by using the stem segment.
Reference numerals: soft sleeve 1, sustained release layer 2.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
A method for grafting tomato sleeves by utilizing stem segments comprises the following steps:
the method comprises the following steps: selecting stock varieties, wherein the tomato stock is selected from Zhejiang stock I with developed root system, strong growth potential, strong disease resistance such as tomato bacterial wilt resistance and stress resistance and good scion affinity;
step two: selecting a scion variety, wherein the scion selection is Ouxiu 201 of a tomato variety which has good grafting affinity with stocks, is disease-resistant, high in yield, high in quality, adapts to local climatic conditions and grows infinitely;
step three: sowing and raising seedlings;
soaking the seeds in 55 deg.C warm water, maintaining the water temperature of 55 deg.C for 30min, stirring to heat the seeds uniformly;
preparing a substrate, wherein the substrate is required to meet the requirements of tomato seed germination and growth, is required to be loose, breathable, water-retaining and fertilizer-retaining, does not have germs and meets the requirements of tomato seedling growth, and a commercial nutrient seedling substrate, such as a Luqing seedling substrate and the like, can be directly adopted;
directly sowing the treated seeds in 50-hole plug trays filled with a matrix, sowing one seed in each hole, sowing the scion 3 days later than the stock, watering and covering a film after sowing, controlling the temperature of a seedbed at 25-30 ℃ in the daytime and 15-20 ℃ at night, removing the film in time after seedling emergence, enhancing the disease and insect control in the seedling stage, controlling the spindling of the seedlings by adopting a tide irrigation technology during the seedling cultivation, screening seedlings in the seedling cultivation management process, enabling the seedlings in the same tray to reach a state of uniform and neat size, and starting grafting when the stock and the scion seedlings grow 6-8 true leaves and 18-22 CM high;
step four: managing in a seedling stage;
the method comprises the steps of building a small arched shed after grafting for heat preservation and moisture preservation, covering thin non-woven fabrics according to weather conditions to avoid direct sunlight, requiring the temperature to be 25-28 ℃ in daytime and 18-20 ℃ at night, keeping the humidity to be 93-95% after grafting, when the temperature in the shed exceeds 30 ℃ and the relative humidity is more than 95%, removing a film to release air and remove moisture, gradually reducing the temperature and the humidity after 3 days, and reducing the temperature and the humidity to be 22-25 ℃ in daytime and 15-18 ℃ at night, keeping the humidity to be 75-80% after ventilation to reduce the humidity in the shed and avoid rotten seedlings, and also preventing overhigh humidity in a seedbed for a long time, removing the small arched shed and the non-woven fabrics after 10-12 days for normal seedling management, strengthening pest control, removing axillary buds of stocks in time, and removing non-survival grafted seedlings and disease-sensitive seedlings until the seedlings survive.
A method for grafting tomato sleeves by utilizing stem sections comprises the following steps that grafting is started when 6-8 true leaves and 18-22 CM in plant height grow on stocks and scion seedlings, and grafting comprises the following steps:
disinfecting shed rooms and seedlings 1 day before grafting, wherein the used medicaments are 400 times of 50% chlorothalonil wettable powder and 600 times of 66.5% propiconazole for sterilization and disinfection;
during grafting, terminal buds and stem sections can be respectively taken according to the height and the number of leaves of a scion seedling to be directly used as a scion, 1-2 true leaves are reserved on each stem section, 2-3 scions can be taken from each plant, a cutting blade is firstly used for upwards beveling at an angle of 35-45 degrees at the position, 6-7 cm away from a base, of the hypocotyl of a selected stock, the section is flat and smooth, a soft sleeve 1 is inserted from the top of the stock cutting face, the lower part of the stock cutting face is located at the middle height position of the soft sleeve 1, the scion seedling with the same diameter as the stock is selected from the selected scions, downwards beveling is carried out at an angle of 35-45 degrees at the position of the hypocotyl, the cut face of the scion seedling is downwards inserted into the soft sleeve 1, and the two bevels of the stock and the scion are tightly combined to obtain the.
A method for grafting tomato by using stem segments is characterized in that a grafting wound rapid healing agent is coated on the contact surface of a soft sleeve 1 and the grafting positions of a stock and a scion. The rapid healing agent can accelerate the healing of grafting wounds, avoid germ infection and effectively improve the grafting survival rate.
A method for grafting tomato by using stem segments, wherein the grafting wound rapid healing agent comprises 50-120 mu M of glucosinolate, 5-10 mu M of zeatin, 5-15 wt% of sucrose, 40mL of ethanol with volume concentration of 95%, and the balance of water.
A method for carrying on the bush grafting of tomato with the stem segment, the inner wall of the soft bush 1 also has a layer of sustained release layer 2, the sustained release substrate material of the sustained release layer 2 chooses chitosan, chitosan can be degraded by muramidase in the organism and produced the natural metabolite, it has non-toxic, can be absorbed the characteristic completely by the organism, therefore use it as sustained release layer substrate have great superiority, the fast healing agent of the grafting wound is smeared and released gradually in the sustained release layer 2.
The average wound healing rate of the plants containing the rapid healing agent slow release layer 2 is 90 percent, and the healing time is 60 hours. The average wound healing rate of the plants without the rapid healing agent slow release layer 2 is lower than 70 percent, and the healing time is longer than 15 days.
A method for grafting a tomato sleeve by utilizing a stem section is characterized in that a soft sleeve 1 is made of degradable materials such as polymerized milk fat and starch plastic, the soft sleeve 1 automatically falls off along with the length of the stem section at the grafting position after the grafting wound of the soft sleeve 1 is completely reserved, the soft sleeve 1 does not need to be taken, and the method is environment-friendly and convenient. The poly-milk fat is a mature degradable material with similar performance to plastic at present, the plastic casing pipe commonly used at present falls into soil after falling off, and the plastic is difficult to be degraded and easily pollutes the environment and the soil. The use of the butterfat can effectively avoid this.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it is therefore intended that the present embodiments be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (1)
1. A method for grafting tomato sleeves by utilizing stem segments is characterized by comprising the following steps:
the method comprises the following steps: selecting stock varieties, wherein the tomato stock is selected from Zhe stock I with developed root system, strong growth potential, high tomato bacterial wilt resistance, strong stress resistance and good scion affinity;
step two: selecting a scion variety, wherein the scion selection is Ouxiu 201 of a tomato variety which has good grafting affinity with stocks, disease resistance, high yield, high quality and unlimited growth;
step three: sowing and raising seedlings;
soaking the seeds in 55 deg.C warm water, maintaining the water temperature of 55 deg.C for 30min, stirring to heat the seeds uniformly;
preparing a substrate, wherein the substrate is required to meet the requirements of tomato seed germination and growth, is loose, breathable, water-retaining and fertilizer-retaining, does not have germs and meets the requirements of tomato seedling growth;
directly sowing the treated seeds in 50-hole plug trays filled with a matrix, sowing one seed in each hole, sowing the scion 3 days later than the stock, watering and covering a film after sowing, controlling the temperature of a seedbed at 25-30 ℃ in the daytime and 15-20 ℃ at night, removing the film in time after seedling emergence, controlling excessive growth of the seedlings by adopting a tide irrigation technology during seedling cultivation, screening seedlings in the seedling cultivation management process, enabling the seedlings in the same tray to reach a state of uniform and neat size, and starting grafting when 6-8 true leaves and 18-22 CM of plant height grow out from the stock and the scion seedlings;
step four: managing in a seedling stage;
building a small arched shed after grafting for heat preservation and moisture preservation, covering thin non-woven fabrics according to weather conditions to avoid direct sunlight, wherein the temperature in the daytime is 25-28 ℃ in the first 3 days after grafting, the humidity at night is 18-20 ℃, the humidity is kept at 93% -95%, when the temperature in the shed exceeds 30 ℃ and the relative humidity is more than 95%, removing a film, ventilating and dehumidifying, gradually reducing the temperature and the humidity after 3 days, the temperature in the daytime is 22-25 ℃, the humidity at night is 15-18 ℃, the humidity is kept at 75% -80%, ventilating is performed to reduce the humidity in the shed, prevent rotten seedlings, prevent overhigh humidity for long time in a seedbed, after 10-12 days, removing the small arched shed and the non-woven fabrics for normal seedling cultivation management, removing axillary buds of the rootstock in time, removing non-viable grafted seedlings and susceptible seedlings until the grafting wounds of the viable seedlings completely heal, grafting wounds are more than 6cm above the ground, and in the third step, when 6-8 true leaves and scion seedlings grow out, Grafting is started when the plant height is 18-22 CM, and the grafting comprises the following steps:
disinfecting shed rooms and seedlings 1 day before grafting, wherein the used medicaments are 400 times of 50% chlorothalonil wettable powder and 600 times of 66.5% propiconazole for sterilization and disinfection;
during grafting, terminal buds and stem sections are respectively taken according to the height and the number of leaves of a scion seedling to be directly used as a scion, 1-2 true leaves are reserved on each stem section, 2-3 scions are taken from each plant, a cutting blade is firstly used at the position 6 CM-7 CM away from a basal position of the hypocotyl of a selected stock and is obliquely cut upwards at an angle of 35-45 degrees, the cut surface is flat and smooth, a soft sleeve is inserted into the top of the cutting surface of the stock, the lower part of the cutting surface of the stock is positioned at the middle height position of the soft sleeve, scion seedlings with the same diameter as the stock are selected from the selected scions, the scion seedlings are obliquely cut downwards at an angle of 35-45 degrees at the position of the hypocotyl, the cut surfaces of the cut scion seedlings are inserted into the soft sleeve downwards, the two oblique cut surfaces of the stock and the scion are tightly combined to obtain a tomato grafting seedling, a grafting wound rapid healing agent is coated on the contact, the grafting wound rapid healing agent comprises 50-120 mu M of glucosinolate, 5-10 mu M of zeatin, 5-15 wt% of sucrose, 40mL of 95% ethanol in volume concentration and the balance of water, a slow release layer is further arranged on the inner wall of the soft sleeve, the grafting wound rapid healing agent is gradually released through the slow release layer, the soft sleeve is made of degradable materials, and the degradable materials are polylactic acid.
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| CN110679313A (en) * | 2019-10-30 | 2020-01-14 | 杭州树联园艺科技有限公司 | Plant grafting method |
| CN115812454B (en) * | 2022-11-16 | 2023-07-18 | 广东省农业科学院蔬菜研究所 | Curing device for healing grafting wound of tomato and curing method thereof |
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| CN102771318A (en) * | 2012-06-19 | 2012-11-14 | 青岛市农业科学研究院 | Grafting cultivation method for tomato by using sleeve pipe |
| CN103416230A (en) * | 2013-08-27 | 2013-12-04 | 遵义市龙泉农业科技发展有限公司 | Disease-resistant, draught-resistant and cold-resistant grafting technology for black tomatoes in South America |
| CN105766397A (en) * | 2016-05-08 | 2016-07-20 | 宁夏天瑞产业集团现代农业有限公司 | Tomato seedling grafting technology and management method thereof |
| CN107087502B (en) * | 2017-07-06 | 2020-09-11 | 中国农业科学院蔬菜花卉研究所 | Method for cultivating double-sprout-tiller grafted seedlings of tomatoes |
| CN107668051A (en) * | 2017-11-30 | 2018-02-09 | 福建农林大学 | A kind of preparation for promoting grafting wound healing |
| CN108184465A (en) * | 2018-03-12 | 2018-06-22 | 成都市农林科学院 | A kind of Tomato Grafting method for culturing seedlings |
| CN108935044A (en) * | 2018-08-01 | 2018-12-07 | 贵州省园艺研究所 | The method for carrying out tomato seedling using tide irrigation seedbed equipment |
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