CN109844606B - 试样观察装置及试样观察方法 - Google Patents

试样观察装置及试样观察方法

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Publication number
CN109844606B
CN109844606B CN201780062595.5A CN201780062595A CN109844606B CN 109844606 B CN109844606 B CN 109844606B CN 201780062595 A CN201780062595 A CN 201780062595A CN 109844606 B CN109844606 B CN 109844606B
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CN
China
Prior art keywords
image data
observation
spim
light
sample
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Application number
CN201780062595.5A
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English (en)
Chinese (zh)
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CN109844606A (zh
Inventor
山本谕
松原正典
杉山范和
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamamatsu Photonics KK
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Hamamatsu Photonics KK
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Publication of CN109844606A publication Critical patent/CN109844606A/zh
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

Landscapes

  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CN201780062595.5A 2016-10-11 2017-08-15 试样观察装置及试样观察方法 Active CN109844606B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016-200075 2016-10-11
JP2016200075A JP6423841B2 (ja) 2016-10-11 2016-10-11 試料観察装置及び試料観察方法
PCT/JP2017/029367 WO2018070098A1 (ja) 2016-10-11 2017-08-15 試料観察装置及び試料観察方法

Publications (2)

Publication Number Publication Date
CN109844606A CN109844606A (zh) 2019-06-04
CN109844606B true CN109844606B (zh) 2025-09-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780062595.5A Active CN109844606B (zh) 2016-10-11 2017-08-15 试样观察装置及试样观察方法

Country Status (6)

Country Link
US (4) US10809509B2 (enExample)
EP (1) EP3528030B1 (enExample)
JP (1) JP6423841B2 (enExample)
CN (1) CN109844606B (enExample)
ES (1) ES2993344T3 (enExample)
WO (1) WO2018070098A1 (enExample)

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JP7207860B2 (ja) * 2018-04-09 2023-01-18 浜松ホトニクス株式会社 試料観察装置
CN115668027A (zh) * 2020-05-27 2023-01-31 浜松光子学株式会社 光照射装置和试样观察装置
EP4137864B1 (en) * 2020-06-01 2025-02-19 Hamamatsu Photonics K.K. Sample observation device and sample observation method
JP7754932B2 (ja) 2021-07-26 2025-10-15 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
JP7746632B1 (ja) 2022-09-09 2025-09-30 シグニファイ ホールディング ビー ヴィ レーザ励起高強度白色光源を製造するためのアキシコンレンズと組み合わせた環状蛍光体
JP2024093373A (ja) 2022-12-27 2024-07-09 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
JP2024093375A (ja) 2022-12-27 2024-07-09 浜松ホトニクス株式会社 試料観察装置及び試料観察方法

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Also Published As

Publication number Publication date
ES2993344T3 (en) 2024-12-27
CN109844606A (zh) 2019-06-04
US20220283420A1 (en) 2022-09-08
EP3528030A4 (en) 2020-05-27
US20200393661A1 (en) 2020-12-17
JP6423841B2 (ja) 2018-11-14
US11391934B2 (en) 2022-07-19
EP3528030B1 (en) 2024-10-09
EP3528030C0 (en) 2024-10-09
US20210382286A1 (en) 2021-12-09
US10809509B2 (en) 2020-10-20
WO2018070098A1 (ja) 2018-04-19
US20200041776A1 (en) 2020-02-06
EP3528030A1 (en) 2019-08-21
US11131839B2 (en) 2021-09-28
US11822066B2 (en) 2023-11-21
JP2018063292A (ja) 2018-04-19

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