CN109844136A - 测序核酸的方法和系统 - Google Patents

测序核酸的方法和系统 Download PDF

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Publication number
CN109844136A
CN109844136A CN201780063633.9A CN201780063633A CN109844136A CN 109844136 A CN109844136 A CN 109844136A CN 201780063633 A CN201780063633 A CN 201780063633A CN 109844136 A CN109844136 A CN 109844136A
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nucleotide
nucleic acid
polymerase
template nucleic
acid molecule
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Chinese (zh)
Inventor
K·维贾亚恩
C·M·丹巴赫尔
E·图
M·A·贝纳德
J·罗基基
K·威尔逊
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Omnium GmbH
Pacific Biosciences of California Inc
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Omniome Inc
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Priority to CN202311235787.5A priority Critical patent/CN117385014A/zh
Publication of CN109844136A publication Critical patent/CN109844136A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN201780063633.9A 2016-08-15 2017-08-15 测序核酸的方法和系统 Pending CN109844136A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311235787.5A CN117385014A (zh) 2016-08-15 2017-08-15 测序核酸的方法和系统

Applications Claiming Priority (5)

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US201662375379P 2016-08-15 2016-08-15
US62/375,379 2016-08-15
US201762447319P 2017-01-17 2017-01-17
US62/447,319 2017-01-17
PCT/US2017/046976 WO2018035134A1 (en) 2016-08-15 2017-08-15 Method and system for sequencing nucleic acids

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US (4) US10443098B2 (enExample)
EP (2) EP4039824A1 (enExample)
JP (2) JP6828140B2 (enExample)
KR (1) KR102230444B1 (enExample)
CN (2) CN109844136A (enExample)
AU (1) AU2017313718B2 (enExample)
WO (1) WO2018035134A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114599795A (zh) * 2019-10-18 2022-06-07 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
CN115710599A (zh) * 2021-08-23 2023-02-24 深圳华大智造科技股份有限公司 去除核苷酸修饰缺陷的方法、试剂盒及在测序中的应用
CN116287167A (zh) * 2023-02-20 2023-06-23 珠海市大道测序生物科技有限公司 核酸分子的测序方法
CN116559427A (zh) * 2023-03-13 2023-08-08 浙江大学 一种基于cmos无透镜成像的侧向流动免疫检测芯片及制备方法、装置及使用方法

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11859171B2 (en) 2013-04-17 2024-01-02 Agency For Science, Technology And Research Method for generating extended sequence reads
US10077470B2 (en) * 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
CN109072297B (zh) * 2016-04-22 2022-09-13 加利福尼亚太平洋生物科学股份有限公司 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统
EP4039824A1 (en) * 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
EP4575499A3 (en) 2017-01-04 2025-07-09 MGI Tech Co., Ltd. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
AU2018210944B2 (en) * 2017-01-20 2021-07-22 Pacific Biosciences Of California, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2019018366A1 (en) 2017-07-18 2019-01-24 Omniome, Inc. METHOD OF CHEMICAL MODIFICATION OF PLASTIC SURFACES
WO2019035897A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. SCANNING APPARATUS AND METHODS USEFUL FOR DETECTION OF CHEMICAL OR BIOLOGICAL ANALYTES
AU2018353136B2 (en) 2017-10-19 2022-05-19 Pacific Biosciences Of California, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
KR20200139671A (ko) 2018-02-06 2020-12-14 옴니옴 인코포레이티드 핵산 프라이머 연장을 위한 조성물 및 기술
WO2019161253A1 (en) 2018-02-16 2019-08-22 Ultima Genomics, Inc. Methods for sequencing with single frequency detection
EP3775259A4 (en) * 2018-03-26 2022-01-05 Ultima Genomics, Inc. Methods of sequencing nucleic acid molecules
AU2019255987A1 (en) 2018-04-19 2020-12-10 Pacific Biosciences Of California, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
WO2019209426A1 (en) 2018-04-26 2019-10-31 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
WO2019231568A1 (en) * 2018-05-31 2019-12-05 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
WO2020023362A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
CN112218877B (zh) 2018-08-27 2025-07-25 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用
GB2574746B (en) * 2018-09-17 2020-06-03 Omniome Inc Engineered polymerases for improved sequencing
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US20240011086A1 (en) * 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
WO2020117653A1 (en) 2018-12-04 2020-06-11 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
CN113227348B (zh) 2018-12-20 2025-05-27 加利福尼亚太平洋生物科学股份有限公司 用于分析核酸和其他分析物的温度控制
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US11680950B2 (en) 2019-02-20 2023-06-20 Pacific Biosciences Of California, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
WO2020181129A1 (en) * 2019-03-07 2020-09-10 The Trustees Of Columbia University In The City Of New York High accuracy nanopore-based single molecule sequencing by synthesis with tagged nucleotides
WO2020206114A1 (en) * 2019-04-02 2020-10-08 The Trustees Of Columbia University In The City Of New York Sequencing by synthesis with energy transfer dye pairs
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
US11180520B2 (en) * 2019-09-10 2021-11-23 Omniome, Inc. Reversible modifications of nucleotides
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
AU2021230282A1 (en) 2020-03-03 2022-09-22 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US20230183798A1 (en) 2020-05-05 2023-06-15 Pacific Biosciences Of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US12505571B2 (en) 2020-08-31 2025-12-23 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
US12469162B2 (en) 2020-08-31 2025-11-11 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US11200446B1 (en) 2020-08-31 2021-12-14 Element Biosciences, Inc. Single-pass primary analysis
CN115812103A (zh) * 2020-11-06 2023-03-17 伊鲁米纳公司 使用寡核苷酸检测混合物中的材料
CA3204784A1 (en) 2021-01-13 2022-07-21 Alex Nemiroski Surface structuring with colloidal assembly
CN117858959A (zh) 2021-05-10 2024-04-09 加利福尼亚太平洋生物科学股份有限公司 滚环扩增期间的dna扩增缓冲液补充
EP4337786A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Single-molecule seeding and amplification on a surface
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
JP2024522795A (ja) 2021-06-18 2024-06-21 エレメント バイオサイエンシズ，インコーポレイティド 操作されたポリメラーゼ
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
EP4532751A1 (en) 2022-06-03 2025-04-09 Element Biosciences, Inc. Compositions and methods for reducing base call errors by removing deaminated nucleotides from a nucleic acid library
WO2023240093A1 (en) 2022-06-06 2023-12-14 Element Biosciences, Inc. Methods for assembling and reading nucleic acid sequences from mixed populations
CN119923478A (zh) 2022-07-05 2025-05-02 元素生物科学公司 使用双链夹板衔接子的无pcr文库制备以及使用方法
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
WO2024040058A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. Methods for preparing nucleic acid nanostructures using compaction oligonucleotides
JP2025530259A (ja) 2022-09-12 2025-09-11 エレメント バイオサイエンシズ，インコーポレイティド ユニバーサル長スプリント鎖を有する二本鎖スプリントアダプター及び使用方法
EP4630490A2 (en) 2022-12-07 2025-10-15 Element Biosciences, Inc. Water soluble cyanine derivatives and their use in methods of sequencing of nucleic acids
CN121712907A (zh) 2023-01-27 2026-03-20 元素生物科学公司 用于使用基于酶的试剂对模板分子的多个区进行测序的组合物和方法
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
AU2024298639A1 (en) 2023-07-24 2026-01-29 Element Biosciences, Inc. On-support circularization and amplification for generating immobilized nucleic acid concatemer molecules
AU2024301610A1 (en) 2023-07-26 2026-02-26 Element Biosciences, Inc. Dna sequencing systems and use thereof
WO2025062341A1 (en) 2023-09-20 2025-03-27 Element Biosciences, Inc. Solid-state and configurable optical test targets and flow cell devices
WO2025120579A1 (en) 2023-12-07 2025-06-12 Element Biosciences, Inc. Compositions and methods for sequencing multiple regions of a template molecule using read-capping nucleotide analogs
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
WO2025147442A1 (en) * 2024-01-02 2025-07-10 Grail, Inc. Rapid bisulfite conversion and recovery of nucleic acid molecules
WO2025163526A1 (en) 2024-01-30 2025-08-07 Element Biosciences, Inc. Method for improving nucleic acid sequencing quality by eliminating nucleic acids with deaminated bases from library and method for sequencing in which complexes of primers, polymerases and labelled probes are bound to concatemers
WO2025165512A1 (en) 2024-02-02 2025-08-07 Element Biosciences, Inc. Amplification-free probe complexes for detection of target analytes
WO2025191535A1 (en) 2024-03-15 2025-09-18 Element Biosciences, Inc. Partially double-stranded splint adaptors and methods of use
WO2025196650A1 (en) 2024-03-19 2025-09-25 Element Biosciences, Inc. Flow cell devices and use thereof
WO2025196727A1 (en) 2024-03-22 2025-09-25 Element Biosciences, Inc. Macromolecular multivalent conjugates and related uses
WO2025196731A1 (en) 2024-03-22 2025-09-25 Element Biosciences, Inc. Polymeric multivalent conjugates and related uses
WO2025212655A1 (en) 2024-04-02 2025-10-09 Element Biosciences, Inc. Multiple priming for on-support nucleic acid amplification
WO2025212654A1 (en) 2024-04-02 2025-10-09 Element Biosciences, Inc. Library molecule titration for tunable surface density in polony sequencing
WO2025235016A1 (en) 2024-05-07 2025-11-13 Element Biosciences, Inc. Sequencing method with spatial flexibility
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme
WO2025240909A1 (en) 2024-05-17 2025-11-20 Element Biosciences, Inc. Hybridization-based capture and enrichment of target nucleic acid sequences
WO2026039088A2 (en) 2024-06-03 2026-02-19 Esbiolab, Llc Excitation and detection methods for analyzing biomolecules
CN121409161A (zh) * 2025-12-29 2026-01-27 西安交通大学 一种衬层及润滑膜厚度同步超声测量方法及系统

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005019476A1 (en) * 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
CN1771336A (zh) * 2003-02-12 2006-05-10 金尼松斯文斯卡股份公司 用于核酸测序的方法和工具
US20090286245A1 (en) * 2008-03-31 2009-11-19 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
WO2010141390A2 (en) * 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
CN103370425A (zh) * 2010-12-17 2013-10-23 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN109072297A (zh) * 2016-04-22 2018-12-21 欧姆尼欧美公司 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6828094B2 (en) 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
ATE225858T1 (de) 1997-07-28 2002-10-15 Medical Biosystems Ltd Sequenzanalyse von nukleinsäuren
US6248539B1 (en) 1997-09-05 2001-06-19 The Scripps Research Institute Porous semiconductor-based optical interferometric sensor
DE69940430D1 (de) 1998-06-24 2009-04-02 Illumina Inc Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US20030064366A1 (en) 2000-07-07 2003-04-03 Susan Hardin Real-time sequence determination
US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU2002241803A1 (en) 2000-10-20 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Transient electrical signal based methods and devices for characterizing molecular interaction and/or motion in a sample
US7371562B2 (en) 2000-10-30 2008-05-13 Sru Biosystems, Inc. Guided mode resonant filter biosensor using a linear grating surface structure
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DE602004021235D1 (de) 2003-02-21 2009-07-09 Geneform Technologies Ltd Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
GB0317343D0 (en) 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
US8071755B2 (en) 2004-05-25 2011-12-06 Helicos Biosciences Corporation Nucleotide analogs
AU2005254984B2 (en) 2004-06-10 2008-10-16 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis
US7264934B2 (en) 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
GB0413082D0 (en) 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
EP1790202A4 (en) 2004-09-17 2013-02-20 Pacific Biosciences California APPARATUS AND METHOD FOR ANALYZING MOLECULES
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7544794B1 (en) 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
US20070009925A1 (en) 2005-05-05 2007-01-11 Applera Corporation Genomic dna sequencing methods and kits
US8192930B2 (en) * 2006-02-08 2012-06-05 Illumina Cambridge Limited Method for sequencing a polynucleotide template
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7678894B2 (en) 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
CN101434988B (zh) 2007-11-16 2013-05-01 深圳华因康基因科技有限公司 一种高通量寡核苷酸测序方法
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CA2720046C (en) 2008-03-31 2018-07-24 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
GB2461026B (en) * 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
TWI408888B (zh) * 2008-08-07 2013-09-11 Ind Tech Res Inst 超音波線性馬達
CN102317471A (zh) 2008-12-11 2012-01-11 加利福尼亚大学董事会 用于单个dna分子的直接测序的方法和系统
EP2379748A4 (en) 2008-12-23 2012-08-29 Illumina Inc MULTIBASE RELEASE FOR LONG READINGS IN SEQUENCING BY SYNTHESIS PROTOCOLS
US20100255487A1 (en) 2009-03-27 2010-10-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US8034923B1 (en) 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US8986930B2 (en) 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
BE1019015A5 (nl) * 2010-12-13 2012-01-10 Luxafoil Energiewerende kunststoffolie.
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2714935B1 (en) 2011-05-27 2017-03-15 Genapsys Inc. Systems and methods for genetic and biological analysis
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
MX342195B (es) 2011-09-13 2016-09-20 Lasergen Inc Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.
CA3104322C (en) 2011-09-23 2023-06-13 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
CN102634586B (zh) 2012-04-27 2013-10-30 东南大学 一种两核苷酸实时合成dna解码测序方法
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
GB201301178D0 (en) 2013-01-23 2013-03-06 Dynamic Biosensors Gmbh Method for sequencing a template nucleic acid immobilized on a substrate
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
EP2971071B1 (en) 2013-03-15 2018-02-28 Illumina, Inc. Enzyme-linked nucleotides
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
EP3241913B1 (en) 2013-07-03 2019-02-20 Illumina, Inc. System for sequencing by orthogonal synthesis
CN105980578B (zh) 2013-12-16 2020-02-14 深圳华大智造科技有限公司 用于使用机器学习进行dna测序的碱基判定器
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
CN107849605B (zh) * 2015-07-21 2021-08-20 欧姆尼欧美公司 核酸测序方法和系统
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
CA3021769C (en) 2016-04-29 2021-11-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
KR102231977B1 (ko) * 2016-04-29 2021-03-24 옴니옴 인코포레이티드 핵산 서열의 결정 방법
EP4039824A1 (en) 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1771336A (zh) * 2003-02-12 2006-05-10 金尼松斯文斯卡股份公司 用于核酸测序的方法和工具
WO2005019476A1 (en) * 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
US20090286245A1 (en) * 2008-03-31 2009-11-19 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
WO2010141390A2 (en) * 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
CN103370425A (zh) * 2010-12-17 2013-10-23 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN109072297A (zh) * 2016-04-22 2018-12-21 欧姆尼欧美公司 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MICHAEL JOHN ROBERT PREVITE等: "DNA sequencing using polymerase substrate-binding kinetics", 《NATURE COMMUNICATIONS》 *
YING LI等: "Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation", 《THE EMBO JOURNAL》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114599795A (zh) * 2019-10-18 2022-06-07 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
CN115710599A (zh) * 2021-08-23 2023-02-24 深圳华大智造科技股份有限公司 去除核苷酸修饰缺陷的方法、试剂盒及在测序中的应用
CN116287167A (zh) * 2023-02-20 2023-06-23 珠海市大道测序生物科技有限公司 核酸分子的测序方法
CN116287167B (zh) * 2023-02-20 2024-06-25 深圳市大道测序生物科技有限公司 核酸分子的测序方法
WO2024174850A1 (zh) * 2023-02-20 2024-08-29 珠海市大道测序生物科技有限公司 核酸分子的测序方法
CN116559427A (zh) * 2023-03-13 2023-08-08 浙江大学 一种基于cmos无透镜成像的侧向流动免疫检测芯片及制备方法、装置及使用方法

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