CN109805212A - A kind of health drink and preparation method thereof with prevention hyperlipidemia function - Google Patents

Abstract

A kind of health drink and preparation method thereof with prevention hyperlipidemia function, it is related to a kind of health drink, it is combined by ultrasonic wave with dynamic countercurrent circulation, ultrasonic wave auxiliary, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in water-soluble functional components, the fluffy middle polyphenols recovery rate of alkali improves 30%-70% compared to traditional extraction process recovery rate, it is external that the fluffy middle polyphenols of alkali can promote lipid and bile acid to exclude, there is remarkable efficacy for hyperlipidemia, polyphenol can reduce the content of cholesterol and triglycerides in blood plasma and liver, it is hardened with prevention of arterial, it reduces blood pressure and blood lipid, the effects of preventing and treating thrombus；Conjugated linoleic acid has antiatherosclerosis, reduces animal and body's cholesterol in common seepweed seeds oil；Have the effects that apparent reducing blood lipid, norcholesterol, antiviral efficacy and prevention cardiovascular disease in conjunction with fructus arctii, highland barley, peppermint, hawthorn etc.；It is suitable for that high fat of blood patient drinks.

Description

A kind of health drink and preparation method thereof with prevention hyperlipidemia function

[technical field]

The present invention relates to a kind of health drink, more particularly, to a kind of health drink with prevention hyperlipidemia function and its Preparation method.

[background technique]

It is a kind of or more in blood plasma that hyperlipemia (Hyperlipidemia) refers to that body fat mass in human body metabolism or transhipment exception make Kind lipid is higher than the metabolic disorder disease of normal level.Clinical manifestation is total cholesterol (TC), triglycerides (TG) in blood It is excessively high or high-density lipoprotein cholesterol (HDL-C) is too low with low-density lipoprotein (LDL-C).Between Past 30 Years, with people Improvement of living standard and life style transformation, the morbidity and mortality of hyperlipemia constantly rise.According to statistics, 2011 Year China hyperlipidemia number of patients is up to 1.6 hundred million.The data of modern medicine diseases monitoring show that the whole world is daily because hyperlipidemia draws As many as nearly 3600 people of the cardiovascular and cerebrovascular disease death toll of hair.

Blood lipid has been accumulated and has resulted in vascular sclerosis, blood vessel blockage in high fat of blood, and then leads to coronary heart disease, cardiac muscle stalk The diseases such as plug, headstroke, nephrosclerosis.It is insufficient due to still there is many people to recognize the danger of hyperlipidemia at present, then increase blood lipid sheet There is no what symptoms for body, and therefore, many people are that inadvertently discovery blood lipid is high.Hyperlipidemia and your diet, living habit Etc. related, since hyperlipidemia can lead to artery sclerosis, best mode is exactly to pass through dietetic regulation, on the one hand reduces cholesterol Intake, on the other hand more intakes achieve the purpose that adjust dietary structure, mode of making the life better to the effective food of reducing blood lipid.

Suaeda salsa is annual herb salification plant, and the fluffy middle nutritional ingredient of alkali is complete and enriches, the fluffy stem leaf protein matter of alkali, Fat, carbohydrate three nutritious elements content are higher, and seed fat content accounts for the 36.5% of dry matter, are higher than soybean, The protein content of fresh and tender cauline leaf accounts for the 40% of dry matter, measures 16 kinds of amino acid in Suaeda salsa altogether, wherein there is 8 kinds of human bodies must Amino acid is needed, and content is higher, there is expansion blood vessel, enhance human immunity, be depressured, the function of hypoglycemic, prevention and cure of cardiovascular disease Effect；The essential trace elements of the human body and vitamin abundant there are many also containing during alkali is fluffy, wherein calcium, magnesium, zinc, iron, nickel content are rich Richness, calcium, phosphorus, iron and core yellow cellulose content are higher than or quite much higher than the vegetables such as spinach, tomato and carrot, Vitamin C content In general vegetables, vitamin B content is 5-8 times of general vegetables, is much higher than spirulina；Invention Announce number is CN106578918A Patent document disclose a kind of fluffy healthy and nutritious noodle of selenium-rich alkali and its production technology, it is fluffy that patent document research demonstrates alkali Middle high 10 times of the more general food of Se content or so, and selenium is referred to as " king of anti-cancer " in body trace element by scientist, tool There is the effect of anticancer, pre- preventing tumor, the generation of hepatopathy, raising body immunity；Invention Announce number are as follows: CN103027962A's Patent document discloses a kind of Suaeda salsa extracting flavonoids and content assaying method, and patent document research demonstrates Suaeda salsa (S.salsa) leaf flavonoids content is higher, and has stronger antioxidant activity, and plant flavone has anti-oxidant, antitumor, anti-dynamic The multiple efficacies such as pulse atherosclerosis, anti-inflammatory.

The one kind of pigment as polyphenol, the pigment during alkali is fluffy are the valuable sources of natural pigment, and the pigment is through researching and analysing For water-soluble anthocyanidin.Anthocyanidin has anti-mutation, resists as natural pigment, security performance with higher The healthcare functions such as oxidation, blood pressure lowering, protection liver, belong to functional food additives.Polyphenols is referred to as " the 7th class battalion Support element ".Polyphenol is natural " antioxidant ", and it is external that polyphenol can promote lipid and bile acid to exclude, and is had for hyperlipidemia aobvious Effect is write, polyphenol can reduce the content of cholesterol and triglycerides in blood plasma and liver, have prevention of arterial hardening, reduce blood pressure The effects of with blood lipid, prevention and treatment thrombus.

In conclusion the fluffy middle polyphenols of alkali has the content for reducing cholesterol and triglycerides in blood plasma and liver, Prevention of arterial hardening, the effect to reduce blood pressure with blood lipid, prevention and treatment thrombus, there are no extract the fluffy middle polyphenol of alkali on the market at present To be applied to the beverage of hyperlipemic patients, and the method for polyphenol haves the defects that recovery rate is low in tradition extraction plant.

[summary of the invention]

In order to overcome the shortcomings in the background art, the present invention develops a kind of health beverage with prevention hyperlipidemia function Material, by ultrasonic wave auxiliary, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in water-soluble function Property ingredient, the fluffy middle polyphenols recovery rate of alkali improve 30%-70% compared to traditional extraction process recovery rate, more during alkali is fluffy It is external that phenolic substances can promote lipid and bile acid to exclude, and has remarkable efficacy for hyperlipidemia, polyphenol can reduce blood plasma and liver The content of dirty middle cholesterol and triglycerides has the effects that prevention of arterial hardening, reduces blood pressure and blood lipid, prevention and treatment thrombus；Alkali Conjugated linoleic acid has antiatherosclerosis, reduces animal and body's cholesterol and triglycerides and low-density in fluffy seed oil Lipoprotein cholesterol can also reduce animal and body fat, increase muscle；Have in conjunction with fructus arctii, highland barley, peppermint, hawthorn etc. The effects of apparent reducing blood lipid, norcholesterol, antiviral efficacy and prevention cardiovascular disease；It is suitable for that high fat of blood patient drinks, There is significant health-care effect especially for hyperlipemic patients.

For achieving the above object, the present invention adopts the following technical scheme:

A kind of health drink and preparation method thereof with prevention hyperlipidemia function, includes the following steps:

The fluffy stem leaf extract preparation of saline-alkali land:

Step 1, the fluffy stem of fresh saline-alkali land and blade are acquired, is put into guarantor's food peace food purifying machine and cleans 1 time~3 Time, removal impurity and agriculture are residual, kill bacterium, guarantee that safety edible is used；

Step 2, by after cleaning Jian Peng stem and blade be put into vegetable-chopper and be cut into every section of 1cm~2cm；

Step 3, the Jian Peng stem cut and blade are mixed with deionized water 1:0.5~1:2, at colloid mill mashing It manages to 30 mesh~50 mesh；

Step 4, continue the slurries in pulverising step 3 to 60 mesh~200 mesh using high-accuracy Wet ultrafine grinding equipment；

Step 5, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1: 20~1:50 adds deionized water, adds 2%~4% (w/w) cellulase, is added 0.05%~0.08% vitamin C, when enzymatic hydrolysis Between 20min~30min, 30 DEG C~50 DEG C of hydrolysis temperature, ultrasonic power 50w~200w, extraction time be 1~2 time, merge leaching Extract；

Step 6, the leaching liquor of step 5 is subjected to coarse filtration, ultrafiltration by reverse osmosis unit, obtains the fluffy cauline leaf of saline-alkali land and extracts Liquid is spare；

The preparation of salt-soda soil common seepweed seeds oil:

Step 7, the fluffy seed of saline-alkali land is acquired, impurity elimination is screened, is sent into cleaning screen after the fluffy seed metering of saline-alkali land and is screened, Remove soil, rubble, seed shell and dust；Through magnetic separator except de-iron is miscellaneous, stone remover removes rubble, clod, and impurity content is small after screening In 0.1%；

Step 8, the fluffy seed of saline-alkali land is put into vacuum oven, uniformly spreads out, is dried in vacuo, vacuum drying temperature Degree is 40-60 DEG C, vacuum degree 10Pa-200Pa, vacuum drying time 3h-6h；

Step 9, the fluffy seed of saline-alkali land is crushed after vacuum drying with pulverizer, is crushed to mesh number as 60 mesh to 150 Mesh；

Step 10, the fluffy powder of seeds of saline-alkali land being subjected to supercritical carbon dioxide extracting, separate mode is the second-order separation, one Grade separating still pressure is 8-10MPa, and the second-order separation kettle pressure is 5-8MPa, and level-one separating still temperature is regulated to 55 DEG C, two fractions 35 DEG C are regulated to from kettle temperature degree, extraction time 2.5h-4.5h；

Step 11, the isolate in level-one separating still and the second-order separation kettle is collected, evaporates separation using spiral evaporator Then ethyl alcohol in object further removes ethyl alcohol in a vacuum drying oven under the conditions of 40 DEG C；

Step 12, the isolate in level-one separating still and the second-order separation kettle is after step 5 removes ethyl alcohol, in level-one separating still Isolate directly as salt-soda soil common seepweed seeds oil, the isolate in the second-order separation kettle is centrifuged with centrifuge, centrifugal rotational speed For 30r/min-50r/min, centrifugation time 20min, upper layer oil reservoir, the saline-alkali land being incorporated in level-one separating still after collection are taken In fluffy seed oil, it is spare that salt-soda soil common seepweed seeds oil is obtained after mixing；

Step 13, the fluffy stem leaf extract of saline-alkali land and salt-soda soil common seepweed seeds oil mix according to the ratio of 9:1 Mixed liquor A；

Step 14, it takes fructus arctii to add appropriate amount of water infusion, filters residue, it is spare to obtain fructus arctii extracting solution, by lotus leaf: peppermint: Cassia Son: hawthorn=1:0.4:0.8:1.5 is weighed adds decocting juice in right amount, after being first boiled by fire, is transferred to small fire and boils altogether 30 minutes, must extract Liquid B is spare；

Step 15, by mixed liquor A in step 13 and step 14, fructus arctii extracting solution, extracting solution B according to 1:0.2:0.8 ratio Example is mixed to prepare mixed liquor C；

Step 16, xylitol is added in mixed liquor C；

Step 17, mixed liquor C carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.

The described health drink and preparation method thereof with prevention hyperlipidemia function, the fluffy stem leaf extract of the alkali its Mineral composition and content are as follows: Ca2+2985 μ g/L~5200 μ g/L, the μ g/L of Mg2+1743 μ g/L~2791, Fe2+162 μ g/ The μ g/L of L~185, the μ g/L of Mn2+5.11 μ g/L~8.03, the μ g/L of Zn+5.27 μ g/L~8.51, μ g/L of K+0.8 μ g/L~3.0, Na + 1746 μ g/L of μ g/L~6899,200 μ of μ g/L~400 g/L of total dissolved solid.

The described health drink and preparation method thereof with prevention hyperlipidemia function, it is anti-described in step 6 and step 17 Osmotic device system is configured that pretreatment system, smart filtration system, corollary system；Pretreatment system is by mechanical filter, absorption Filter, cartridge filter composition；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system is by water purification pot, electric appliance Control system composition；Mechanical filter filtering can remove body refuse and fiber etc. in extracting solution, after filtered through adsorption filter Oil removal can be removed, however the impurity such as algae can be removed through mechanical filter filtering.

The health drink and preparation method thereof that there is prevention hyperlipidemia function, ultrafiltration described in step 6 and step 17 It is hollow-fibre membrane ultrafiltration to remove the microorganisms such as bacterium in extracting solution, mould.

The health drink and preparation method thereof for having prevention hyperlipidemia function, the hollow-fibre membrane diameter are 0.01 μm~0.001 μm, feed rate is 900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration 200 The μ g/L of μ g/L~400, ultrafiltration time 100min~400min, backwash cycle 10min~40min, backwash time 20min~ 120min, upper operation 10min~40min, upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.

Due to the adoption of the technical scheme as described above, the invention has the following beneficial effects:

1, it is the food of " dual-purpose of drug and food " that lotus leaf is selected in the health drink ingredient with prevention hyperlipidemia function of the invention Object, the Flavonoid substances being rich in lotus leaf, is the scavenger of most of oxygen radicals, SOD (superoxide dismutase can be improved Enzyme) vigor, reduce the generation of MDA (malondialdehyde) and 0X-LDL (OxLDL ELISA), it can be with Increase coronary flow, have antagonism to Myocardial Infarction, have protective effect to acute myocardial ischemia, to treatment coronary disease Disease, hypertension etc. have remarkable result, and to diastolic pressure is reduced, prevention and treatment arrhythmia cordis, cardiovascular disease etc. also play an important role, in addition, Another major class active material-alkaloid in lotus leaf, physiological activity is significant, has apparent reducing blood lipid, antiviral and other effects, in Medicine thinks that lotus leaf nature and flavor are bitter, puts down, returns liver, spleen, stomach, the heart channel of Hang-Shaoyin, there is clearing summer-heat and damp, sending up the lucid yang, cooling blood and hemostasis and other effects, Chinese medicine modern study the result shows that, lotus leaf has effect for reducing blood fat；Radix bardanae promotes blood circulation, removes stomach rubbish, prevents Human body, which crosses presenility, reduction cholesterol and blood glucose, the fiber of fructus arctii, can promote intestinal peristalsis, reduce internal cholesterol, reduce The effect of toxin, waste accumulate in vivo, reach pre- anti-stroke and prevent and treat gastric cancer, uterine cancer, radix bardanae contains multivitamin With minerals abundant, according to surveying and determination, 4.1-4.7 containing protein grams in every 100 grams of radix bardanaes, 3.0-3.5 grams of carbohydrate, calcium 240 milligrams, 0.1 gram of fat, 1.3-1.5 grams of cellulose, 390 milligrams of carrotene, wherein carotene carotene content occupies the in vegetables Two, 150 times are higher by than carrot；Peppermint: nature and flavor are pungent, cool, return liver and lung channel, have diverging wind-heat, head clearing, relieving sore-throat saturating The effect of rash has the function of reducing temperature of heatstroke prevention, diuresis, reducing liver heat, Detoxicating food-poisoning, reducing blood lipid, refreshes the mind；Cassia seed, mildly bitter flavor, With clearing liver and improving vision, sharp water defaecation plays the role of laxative, lowering blood pressure and blood fat；Hawthorn energy prevention and cure of cardiovascular disease has and expands Blood vessel increases coronary blood flow, improves heart vigor, stimulating central nervous system system, reducing blood pressure and cholesterol, softening blood vessel And diuresis and sedation；It is external that the fluffy middle polyphenols of alkali can promote lipid and bile acid to exclude, and has for hyperlipidemia aobvious Effect is write, polyphenol can reduce the content of cholesterol and triglycerides in blood plasma and liver, have prevention of arterial hardening, reduce blood pressure The effects of with blood lipid, prevention and treatment thrombus；Conjugated linoleic acid has antiatherosclerosis, reduces animal and human body gallbladder in common seepweed seeds oil Sterol and triglycerides and low density lipoprotein cholesterol can also reduce animal and body fat, increase muscle；It is suitable for height Blood lipid patient drinks, and has significant health-care effect especially for hyperlipemic patients.

2, in the fluffy extracting solution of alkali simultaneously alkali it is fluffy in also rich in the mineral elements such as calcium, magnesium, iron, manganese, zinc, copper, strontium, sodium, and Containing abundant amino acid classes, up to 18 kinds, including must amino acid: threonine, valine, isoleucine, methionine, Leucine, phenylalanine, lysine, tryptophan.Nonessential amino acid: asparatate, serine, glutamic acid, glycine, third Propylhomoserin, cystine, tyrosine, histidine, arginine, proline.Wherein essential amino acids content accounts for total amino acid content difference 35.2%, the reference model (36%) proposed close to FAO, it is necessary to which the ratio of amino acid and nonessential amino acid is 55%, is approached The reference model (0.56) that FAO is proposed, can provide comprehensive and abundant nutrition for liver and gallbladder patient.

3, the fluffy middle polyphenol recovery rate of alkali of the present invention improves 30%-70% compared to traditional extraction process recovery rate, passes through Ultrasonic wave is combined with dynamic countercurrent circulation, so that the fluffy middle polyphenol recovery rate of alkali greatly improves, is played a multiplier effect；It is logical Cross ultrasonic wave auxiliary, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in it is water-soluble it is functional at Point, the fluffy middle polyphenols recovery rate of alkali, aqueous enzymatic method combination ultrasonic wave physical process, using the very big acceleration of its generation, by force The properties such as strong cavitation effect, high vibration are reduced and are asked when extracting, and temperature is avoided to change the activity change to effective component；This The ultrasonic wave dynamic countercurrent auxiliary enzymes and ultrafiltration technology of invention combine the method production work for extracting the fluffy middle polyphenols of alkali Skill is simple, the consuming time is few, reduces production work intensity, has product effective component high, and it is unfavorable to reduce temperature change bring The advantages that influence.

4, the present invention using coarse filtration and ultrafiltration effectively eliminate the impurity such as body refuse, suspended matter, algae in extracting solution and The microorganisms such as bacterium, mould in extracting solution, high degree remain the fluffy extracting solution of alkali, highland barley liquid, peppermint, hawthorn, lotus leaf, determine The various nutritional ingredients and microelement of pine torch extracting solution, it is easy to operate, it is suitable for industrialized production, compared to traditional filtering side Method, hyperfiltration technique are a kind of purifications for being widely used in water, and pollution-free, low energy consumption, separator is simple, process is short, it is easy to operate, Easily controllable and maintenance, installation area are small.It carries out at normal temperature, mild condition is thus particularly suitable right without component damage The separation and enrichment of the substance of this product (thermo-responsive), it is highly effective to the recycling of the micro constitutent in this product.Ultrafiltration technology Mechanical filter filtering can remove body refuse and fiber etc. in extracting solution in pretreatment system, after can through adsorption filter filtering To remove the impurity such as oil removal and algae, and then mentioned by the hollow-fibre membrane ultrafiltration of ultrafiltration technology essence filtration system with removing Take the microorganisms such as bacterium in liquid, mould.

5, the fluffy polyphenols recovery rate of alkali may be up to 5.9% in the present invention, and polyphenols is referred to as " the 7th class nutrition Element ", it is external that the fluffy middle polyphenols of alkali can promote lipid and bile acid to exclude, and has remarkable efficacy, polyphenol energy for hyperlipidemia The content of cholesterol and triglycerides in blood plasma and liver is reduced, there is prevention of arterial hardening, reduce blood pressure and blood lipid, prevention and treatment blood The effects of bolt.It is suitable for that high fat of blood patient drinks, there is significant health-care effect especially for hyperlipemic patients.

6, the present invention is directed to the critical CO of the fluffy seed part over-extraction of saline-alkali land₂Extraction has extraction efficiency high, solvent-free Residual toxicity, active skull cap components and heat-sensitive ingredients are not easy the destruction that is decomposed, and can keep the natural of extract to the maximum extent Feature is, it can be achieved that the advantages of Selective Separation.

7, the fluffy raw material of alkali chooses alkali fluffy plant of the growth cycle within 45 days in the present invention, and the fluffy cauline leaf of alkali at this time is fresh Tender, rich in nutritional ingredients such as functional pigmented, vitamin, protein and alkaloids, the fluffy seed of saline-alkali land selects 10- in the present invention November the fluffy seed of saline-alkali land, in addition, the fluffy raw material of alkali selected by the present invention is artificial cultivation, avoiding wild suaeda glauce raw material may Existing heavy metals exceeding standard problem, artificial cultivation, good growing environment ensure that the safety of raw material.

8, the present invention, which has, is free of sucrose in the health drink of prevention hyperlipidemia function, pure plant extracting solution uses Xylitol, it is green safe.

9, physical and chemical index detection is carried out to the health drink with prevention hyperlipidemia function provided by the invention, it is every main Index is all satisfied requirements of the national standard.

[Detailed description of the invention]

Fig. 1 is influence of the liquid-solid ratio to the fluffy pigment extraction effect of alkali；

Fig. 2 is the response surface that solid-liquid ratio, cellulase, vitamin C and ultrasonic power influence polyphenol content.

[specific embodiment]

The present invention can be explained in more detail by the following examples, the open purpose of the present invention is intended to protect the present invention All changes and improvements in range, the invention is not limited to the following examples；

A kind of health drink and preparation method thereof with prevention hyperlipidemia function, includes the following steps:

The fluffy stem leaf extract preparation of saline-alkali land:

Step 1, the fluffy stem of fresh saline-alkali land and blade are acquired, is put into guarantor's food peace food purifying machine and cleans 1 time~3 Time, removal impurity and agriculture are residual, kill bacterium, guarantee that safety edible is used；

Step 2, by after cleaning Jian Peng stem and blade be put into vegetable-chopper and be cut into every section of 1cm~2cm；

Step 3, the Jian Peng stem cut and blade are mixed with deionized water 1:0.5~1:2, at colloid mill mashing It manages to 30 mesh~50 mesh；

Step 4, continue the slurries in pulverising step 3 to 60 mesh~200 mesh using high-accuracy Wet ultrafine grinding equipment；

Step 5, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1: 20~1:50 adds deionized water, adds 2%~4% (w/w) cellulase, is added 0.05%~0.08% vitamin C, when enzymatic hydrolysis Between 20min~30min, 30 DEG C~50 DEG C of hydrolysis temperature, ultrasonic power 50w~200w, extraction time be 1~2 time, merge leaching Extract；

Step 6, the leaching liquor of step 5 is subjected to coarse filtration, ultrafiltration by reverse osmosis unit, obtains the fluffy cauline leaf of saline-alkali land and extracts Liquid is spare；

The preparation of salt-soda soil common seepweed seeds oil:

Step 7, the fluffy seed of saline-alkali land is acquired, impurity elimination is screened, is sent into cleaning screen after the fluffy seed metering of saline-alkali land and is screened, Remove soil, rubble, seed shell and dust；Through magnetic separator except de-iron is miscellaneous, stone remover removes rubble, clod, and impurity content is small after screening In 0.1%；

Step 8, the fluffy seed of saline-alkali land is put into vacuum oven, uniformly spreads out, is dried in vacuo, vacuum drying temperature Degree is 40-60 DEG C, vacuum degree 10Pa-200Pa, vacuum drying time 3h-6h；

Step 9, the fluffy seed of saline-alkali land is crushed after vacuum drying with pulverizer, is crushed to mesh number as 60 mesh to 150 Mesh；

Step 10, the fluffy powder of seeds of saline-alkali land being subjected to supercritical carbon dioxide extracting, separate mode is the second-order separation, one Grade separating still pressure is 8-10MPa, and the second-order separation kettle pressure is 5-8MPa, and level-one separating still temperature is regulated to 55 DEG C, two fractions 35 DEG C are regulated to from kettle temperature degree, extraction time 2.5h-4.5h；

Step 11, the isolate in level-one separating still and the second-order separation kettle is collected, evaporates separation using spiral evaporator Then ethyl alcohol in object further removes ethyl alcohol in a vacuum drying oven under the conditions of 40 DEG C；

Step 12, the isolate in level-one separating still and the second-order separation kettle is after step 5 removes ethyl alcohol, in level-one separating still Isolate directly as salt-soda soil common seepweed seeds oil, the isolate in the second-order separation kettle is centrifuged with centrifuge, centrifugal rotational speed For 30r/min-50r/min, centrifugation time 20min, upper layer oil reservoir, the saline-alkali land being incorporated in level-one separating still after collection are taken In fluffy seed oil, it is spare that salt-soda soil common seepweed seeds oil is obtained after mixing；

Step 13, the fluffy stem leaf extract of saline-alkali land and salt-soda soil common seepweed seeds oil mix according to the ratio of 9:1 Mixed liquor A；

Step 14, it takes fructus arctii to add appropriate amount of water infusion, filters residue, it is spare to obtain fructus arctii extracting solution, by lotus leaf: peppermint: Cassia Son: hawthorn=1:0.4:0.8:1.5 is weighed adds decocting juice in right amount, after being first boiled by fire, is transferred to small fire and boils altogether 30 minutes, must extract Liquid B is spare；

Step 15, by mixed liquor A in step 13 and step 14, fructus arctii extracting solution, extracting solution B according to 1:0.2:0.8 ratio Example is mixed to prepare mixed liquor C；

Step 16, xylitol is added in mixed liquor C；

Step 17, mixed liquor C carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.

The described health drink and preparation method thereof with prevention hyperlipidemia function, the fluffy stem leaf extract of the alkali its Mineral composition and content are as follows: Ca2+2985 μ g/L~5200 μ g/L, the μ g/L of Mg2+1743 μ g/L~2791, Fe2+162 μ g/ The μ g/L of L~185, the μ g/L of Mn2+5.11 μ g/L~8.03, the μ g/L of Zn+5.27 μ g/L~8.51, μ g/L of K+0.8 μ g/L~3.0, Na + 1746 μ g/L of μ g/L~6899,200 μ of μ g/L~400 g/L of total dissolved solid.

The described health drink and preparation method thereof with prevention hyperlipidemia function, it is anti-described in step 6 and step 17 Osmotic device system is configured that pretreatment system, smart filtration system, corollary system；Pretreatment system is by mechanical filter, absorption Filter, cartridge filter composition；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system is by water purification pot, electric appliance Control system composition；Mechanical filter filtering can remove body refuse and fiber etc. in extracting solution, after filtered through adsorption filter Oil removal can be removed, however the impurity such as algae can be removed through mechanical filter filtering.

The health drink and preparation method thereof that there is prevention hyperlipidemia function, ultrafiltration described in step 6 and step 17 It is hollow-fibre membrane ultrafiltration to remove the microorganisms such as bacterium in extracting solution, mould.

The health drink and preparation method thereof for having prevention hyperlipidemia function, the hollow-fibre membrane diameter are 0.01 μm~0.001 μm, feed rate is 900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration 200 The μ g/L of μ g/L~400, ultrafiltration time 100min~400min, backwash cycle 10min~40min, backwash time 20min~ 120min, upper operation 10min~40min, upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.

Wherein, water used is deionized water, and the minerals in water have been filtered out in deionized water, have avoided calcium in water, magnesium The interference of plasma.

Addition vitamin C can play the color protection fluffy to alkali, anti-oxidation efficacy in steps of 5, while also can be realized battalion Support the purpose strengthened.

The detection of every physical and chemical index:

For nutritional ingredient composition and content situation in the fluffy stem leaf extract of saline-alkali land and salt-soda soil common seepweed seeds oil, pass through Micro-kjoldahl method measures crude protein content；Using determination of crude oil by Soxhlet extraction；It is thick using gravimetry Fiber content；Moisture content is measured using atmosphere pressure desiccation；Carbohydrate content is measured using anthrone colorimetry；Using high temperature Ash purification determining content of ashes；Amino acid content is measured using Hitachi 835-50 type high-speed amino acid analyzer；Using fluorescence point Light photometry measures tryptophan composition and content；Using atomic absorption spectroscopy determination mineral element, wherein selenium element is used Fluorescence spectrophotometry；Every nutritional ingredient and content are shown in Table 1-3 in the common seepweed seeds oil of salt-soda soil, and the fluffy cauline leaf of saline-alkali land mentions Every nutritional ingredient and content in liquid is taken to be shown in Table 4-6:

Main nutrient composition content in 1 salt-soda soil common seepweed seeds oil of table

2 salt-soda soil common seepweed seeds oil Mineral Element Contents (μ g/g) of table

The composition and content (mg/g) of amino acid in 3 salt-soda soil common seepweed seeds oil of table

The fluffy stem leaf extract nutrient content of 4 saline-alkali land of table

The fluffy stem leaf extract Mineral Elements in Jadeite Shellfish content of 5 alkali of table

The fluffy stem leaf extract amino acid content of 6 saline-alkali land of table

Pass through nutritional ingredient composition and content situation in the fluffy stem leaf extract of measurement saline-alkali land and salt-soda soil common seepweed seeds oil It can be seen that

The comparison of table 1 and table 4 can be seen that crude protein, crude fat content in the common seepweed seeds oil of salt-soda soil: 18.75% and 23.46%, much higher than crude protein, crude fat content in the fluffy stem leaf extract of saline-alkali land: 3.020% and 1.380%, and salt Alkali in common seepweed seeds oil crude fat content account for 23.46%, be higher than soybean (crude fat content 18.80%), be good plant Oil sources can be used as good protein sources, vitamin in the fluffy stem leaf extract of saline-alkali land since its crude protein content is also higher Content is higher, in addition, the chemotrophy ingredient of the fluffy seed of saline-alkali land and the fluffy cauline leaf of saline-alkali land is complete and enriches.

The comparison of table 2 and table 5 can be seen that salt-soda soil common seepweed seeds oil Mineral Elements potassium and the content of sodium is the abundantest, This is related with the saline-alkali environment that it grows, close with human health in salt-soda soil common seepweed seeds oil and the fluffy stem leaf extract of saline-alkali land It is higher to cut the constituent contents such as relevant iron, calcium, zinc, copper, manganese, it is seen then that the fluffy seed of saline-alkali land and the fluffy cauline leaf of saline-alkali land are one Kind good mineral matter nutritional source, in addition, also contain selenium in the common seepweed seeds oil of salt-soda soil, it is micro that selenium by scientist is referred to as human body " king of anti-cancer " in element therefore develops the fluffy seed of saline-alkali land and can be the mankind using the selenium contained by the fluffy seed of saline-alkali land Bring high value medical health care.

The comparison of table 3 and table 6, which can be seen that in salt-soda soil common seepweed seeds oil and the fluffy stem leaf extract of saline-alkali land, contains 18 Middle amino acid, rich content, it is necessary to which amino acid composition is complete, and wherein it is total to account for amino acid for the fluffy seed essential amino acids content of saline-alkali land The 36.44% of amount is higher than FAO mode (36.00%), while the ratio between the fluffy seed essential amino acid of saline-alkali land and nonessential amino acid It (E/N) is 0.573, also above FAO mode (0.56), it is seen then that the fluffy seed albumen of alkali belongs to quality plant albumen；The fluffy stem of saline-alkali land It is 35.5% that essential amino acids content, which accounts for total amino acid content, in leaf, the reference model (36%) proposed close to FAO, it is necessary to amino acid Ratio with nonessential amino acid is 55.1%, the reference model (0.56) proposed close to FAO；In addition, the research discovery fluffy seed of alkali The common seepweed seeds oil obtained after processing is refined has the physicochemical property of general edible oil, and it is next to can be used as a kind of new edible oil Exploitation, it is rich in unsaturated fatty acid in addition, and especially linoleic acid content is higher than many plants, it can be seen that, common seepweed seeds oil is one Kind of vegetable oil new resources containing high linoleic acid, have important a nutrition health-care functions and biggish Development volue, in common seepweed seeds oil Rich in unsaturated fatty acid, total unsaturated fatty acid content is up to 90.65%, and wherein linoleic acid content accounts for 68.74%, is current It was found that one of the higher oil sources of linoleic acid content, physical and chemical index also substantially conforms to edible oil standard, is a kind of ideal The good material of edible health-care oil and preparation conjugated linoleic acid.

By above-mentioned analysis it is found that the nutritional ingredient being rich in by the fluffy seed of saline-alkali land and the fluffy stem and leaf part of saline-alkali land It is biased to difference, the present invention can be made by deploying salt-soda soil common seepweed seeds oil and the fluffy stem leaf extract of saline-alkali land in proportion Have the health drink of prevention hyperlipidemia function, fluffy middle the be rich in nutritional ingredient effect of alkali is maximized, is sent out to greatest extent Wave the fluffy edible value of saline-alkali land.

Preparation method described in Example preparation have prevention hyperlipidemia function health drink, by GB/T12143.1, GB8537-1995, GB4789 examination criteria compare indices, the results are shown in Table 7.

Table 7 has the health drink indices of prevention hyperlipidemia function

It is visible according to the health drink items main indicator with prevention hyperlipidemia function prepared by the present invention by 7 result of table It is all satisfied requirements of the national standard.

In order to determine the solid-liquid ratio in the fluffy stem leaf extract preparation process of alkali, the present invention is directed to the feed liquid of alkali fluffy cauline leaf and water Than carrying out experimental study, liquid-solid ratio is obtained to the influence diagram of the fluffy pigment extraction effect of alkali, as shown in Figure 1, as shown in Figure 1, with The absorbance of the increase of liquid-solid ratio, pigment solution gradually rises, and when liquid-solid ratio is 40:1, light absorption value is significantly increased, and obtains alkali Fluffy pigment recovery rate also significantly increases, and when liquid-solid ratio is greater than 40:1, curve tends towards stability, and absorbance changes less, and synthesis is examined Consider energy-saving and emission-reduction, selects liquid-solid ratio for 40:1 (mL/g).

On the basis of above-mentioned single factor experiment, solid-liquid ratio, 4 cellulase, vitamin C and ultrasonic power factors are chosen Box-Behnken design is carried out, the maximum value of polyphenol content in the fluffy stem leaf extract of alkali is studied, the results are shown in Table 8:

Table 8Box-Behnken experimental design scheme and result

Regression fit is carried out to 8 test data of table using 8.0 design software of Design-Expert, obtains polyphenol content pair Solid-liquid ratio, cellulase, 4 factors of vitamin C and ultrasonic power secondary multinomial regression model:

Polyphenol content=5.55+0.13A+0.19B+0.32C+0.19D-0.33A²-0.39B²-0.28C²-0.96D²

9 response surface design quadratic regression equation model the results of analysis of variance of table

Note: * significant difference p < 0.05；The extremely significant p < 0.01 of * difference.

Make response surface figure according to regression equation, investigate the shape of be fitted response surface, analysis solid-liquid ratio, cellulase, Fig. 2 is met personally in the influence of vitamin C and ultrasonic power to polyphenol content, response.As shown in Table 9, mutation model p value < 0.0001, Reach extremely significant level, illustrates that the models fitting degree is fine.It as shown in Figure 2, is 1:50, cellulase 3%, dimension in solid-liquid ratio Raw element C 0.05%, ultrasonic power 125w, polyphenol content reach peak, and the result and experiment of single factor result one It causes.

It is above-mentioned the experimental results showed that, the present invention passes through ultrasonic wave auxiliary, aqueous enzymatic extraction and ultrafiltration technology triple combination pole It is big remain alkali it is fluffy in water-soluble functional components, polyphenols recovery rate is compared to traditional extraction side in the fluffy cauline leaf of alkali Method recovery rate improves 30%-70%, and the fluffy polyphenol recovery rate of alkali of the invention may be up to 5.9%, the fluffy middle polyphenols energy of alkali Promote lipid and bile acid to exclude external, there is remarkable efficacy for hyperlipidemia, polyphenol can reduce cholesterol in blood plasma and liver With the content of triglycerides, has the effects that prevention of arterial hardening, reduces blood pressure and blood lipid, prevention and treatment thrombus；In common seepweed seeds oil altogether Conjugated linoleic acid has antiatherosclerosis, reduction animal and body's cholesterol and triglycerides and low-density lipoprotein gallbladder solid Alcohol can also reduce animal and body fat, increase muscle；There is apparent drop blood in conjunction with lotus leaf, fructus arctii, peppermint, hawthorn etc. The effects of rouge, norcholesterol, antiviral efficacy and prevention cardiovascular disease；It is suitable for that high fat of blood patient drinks, especially for height Blood lipid patient has significant health-care effect.

The health drink clinical observation data with prevention hyperlipidemia function produced by the present invention is as follows:

XX is opened, it is male, 56 years old.Often occur feeling dizzy or be easy with people's speech gap sleeping.Feel brains after getting up in the morning Do not regain consciousness, it is early to improve after the meal, it easily feels sleepy in the afternoon, but night is very awake.It has a pain in the back, shortness of breath uncomfortable in chest, headache, dizziness, note Recall power decline.Health drink of the present invention is drunk, 3 times a day, each 220ml persistently takes one month, and sleep restores normal, note Recall power to be obviously improved, have a headache, dizziness is obviously improved.

Liu XX, female 48 years old, for many years with hyperlipidemia, Symptoms are as follows: dizziness, spiritlessness and weakness, insomnia forgetfulness, limbs fiber crops Wooden, uncomfortable in chest, palpitaition etc..Blood lipid leans on always the drug of reducing blood lipid to maintain, as long as soon as usually blood pressure drug stops, blood lipid increases quickly. Health drink of the present invention is drunk, 3 times a day, each 220ml after persistently taking 4 weeks, goes to hospital to check that blood lipid, index restore just Often.The symptoms such as dizzy, out of strength are all clearly better, and extremity numbness symptom also makes moderate progress.

King XX, male, 65 years old.It often feels dizzy and is easy to feel sleepy, and blackspot increases, failure of memory, blurred vision, waist Sour backache, shortness of breath uncomfortable in chest, insomnia and dreamful sleep.Health drink of the present invention is drunk, 3 times a day, each 220ml, after persistently taking 6 weeks, Sleep quality improves, no longer insomnia and dreamful sleep, and eyesight is gradually recovered, and sees that thing is also apparent from.

Part not in the detailed description of the invention is the prior art.

Claims (5)

1. a kind of health drink and preparation method thereof with prevention hyperlipidemia function, it is characterized in that: including the following steps:

The fluffy stem leaf extract preparation of saline-alkali land:

Step 1, the fluffy stem of fresh saline-alkali land and blade are acquired, is put into guarantor's food peace food purifying machine and cleans 1 time~3 times, go Except impurity and agriculture are residual, kill bacterium, guarantee that safety edible is used；

Step 2, by after cleaning Jian Peng stem and blade be put into vegetable-chopper and be cut into every section of cm of 1 cm~2；

Step 3, the Jian Peng stem cut and blade are mixed with deionized water 1:0.5~1:2, using colloid mill mashing handle to 30 mesh~50 mesh；

Step 4, continue the slurries in pulverising step 3 to 60 mesh~200 mesh using high-accuracy Wet ultrafine grinding equipment；

Step 5, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1:20~ 1:50 adds deionized water, adds 2%~4%(w/w) cellulase, 0.05%~0.08% vitamin C, 20 min of enzymolysis time is added ~30 min, 30 DEG C~50 DEG C of hydrolysis temperature, the ultrasonic power w of 50 w~200, extraction time is 1~2 time, merges extraction Liquid；

Step 6, the leaching liquor of step 5 is subjected to coarse filtration, ultrafiltration by reverse osmosis unit, it is standby obtains the fluffy stem leaf extract of saline-alkali land With；

The preparation of salt-soda soil common seepweed seeds oil:

Step 7, the fluffy seed of saline-alkali land is acquired, impurity elimination is screened, is sent into cleaning screen after the fluffy seed metering of saline-alkali land and is screened, removed Soil, rubble, seed shell and dust；Through magnetic separator except de-iron is miscellaneous, stone remover removes rubble, clod, and impurity content is less than after screening 0.1%；

Step 8, the fluffy seed of saline-alkali land is put into vacuum oven, uniformly spreads out, is dried in vacuo, vacuum drying temperature is 40-60 DEG C, vacuum degree 10Pa-200Pa, vacuum drying time 3h-6h；

Step 9, the fluffy seed of saline-alkali land is crushed with pulverizer after vacuum drying, being crushed to mesh number is 60 mesh to 150 mesh；

Step 10, the fluffy powder of seeds of saline-alkali land is subjected to supercritical carbon dioxide extracting, separate mode is the second-order separation, a fraction It is 8-10MPa from kettle pressure, the second-order separation kettle pressure is 5-8MPa, and level-one separating still temperature is regulated to 55 DEG C, the second-order separation kettle Temperature is regulated to 35 DEG C, extraction time 2.5h-4.5h；

Step 11, the isolate in level-one separating still and the second-order separation kettle is collected, is evaporated in isolate using spiral evaporator Ethyl alcohol, then under the conditions of 40 DEG C in a vacuum drying oven further removal ethyl alcohol；

Step 12, point of the isolate in level-one separating still and the second-order separation kettle after step 5 removes ethyl alcohol, in level-one separating still From object directly as salt-soda soil common seepweed seeds oil, the isolate in the second-order separation kettle is centrifuged with centrifuge, and centrifugal rotational speed is 30r/min -50r/min, centrifugation time 20min take upper layer oil reservoir, the saline-alkali land being incorporated in level-one separating still after collection In fluffy seed oil, it is spare that salt-soda soil common seepweed seeds oil is obtained after mixing；

Step 13, it carries out the fluffy stem leaf extract of saline-alkali land and salt-soda soil common seepweed seeds oil to mix to obtain mixing according to the ratio of 9:1 Liquid A；

Step 14, it takes fructus arctii to add appropriate amount of water infusion, filters residue, it is spare to obtain fructus arctii extracting solution, by lotus leaf: peppermint: cassia seed: mountain Short, bristly hair or beard=1:0.4:0.8:1.5 is weighed adds decocting juice in right amount, after being first boiled by fire, is transferred to small fire and boils altogether 30 minutes, it is standby to obtain extracting solution B With；

Step 15, by mixed liquor A in step 13 and step 14, fructus arctii extracting solution, extracting solution B is mixed according to the ratio of 1:0.2:0.8 It closes and mixed liquor C is made；

Step 16, xylitol is added in mixed liquor C；

Step 17, mixed liquor C carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.

2. the health drink and preparation method thereof with prevention hyperlipidemia function according to claim 1, it is characterized in that: institute Its mineral composition of the fluffy stem leaf extract of the alkali stated and content are as follows: the 2985 μ g/L of μ g/L~5200 of Ca2+, 1743 Mg2+ The μ g/L of μ g/L~2791,162 the Fe2+ μ g/L of μ g/L~185,5.11 the Mn2+ μ g/L of μ g/L~8.03,5.27 Zn+ μ The μ g/L of g/L~8.51,0.8 the K+ μ g/L of μ g/L~3.0,1746 Na+ μ g/L of μ g/L~6899, total dissolved solid 200 The μ of μ g/L~400 g/L.

3. the health drink and preparation method thereof with prevention hyperlipidemia function according to claim 1, it is characterized in that: step Rapid 6 and step 17 described in reverse osmosis unit system configuration are as follows: pretreatment system, smart filtration system, corollary system；Pretreatment system System is made of mechanical filter, adsorption filter, cartridge filter；Smart filtration system is made of hollow-fibre ultrafiltration device；Match Set system is made of water purification pot, electrical control system；Mechanical filter filtering can remove body refuse and fiber etc. in extracting solution, it Oil removal can be removed by adsorption filter filtering, however the impurity such as algae can be removed through mechanical filter filtering.

4. the health drink and preparation method thereof with prevention hyperlipidemia function according to claim 3, it is characterized in that: step Rapid 6 and step 17 described in ultrafiltration be hollow-fibre membrane ultrafiltration to remove the microorganisms such as bacterium in extracting solution, mould.

5. the health drink and preparation method thereof with prevention hyperlipidemia function according to claim 3 or 4, feature Be: the hollow-fibre membrane diameter is 0.01 μm~0.001 μm, and feed rate is the L/h of 900 L/h~1500, pressure The Mpa of 0.045 Mpa~0.18, feed concentration be the 200 μ g/L of μ g/L~400, ultrafiltration time 100 min~400 min, instead Wash period 10 min~40 min, backwash time 20 min~120 min, upper operation 10 min~40 min, 20 s of upper backwash ~120 s, lower operation 10 min~40 min, lower backwash 20 s~120 s.