CN109628265A - A kind of liver-protection health-care molecule wine and preparation method thereof containing general flavone - Google Patents
A kind of liver-protection health-care molecule wine and preparation method thereof containing general flavone Download PDFInfo
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- CN109628265A CN109628265A CN201910019926.8A CN201910019926A CN109628265A CN 109628265 A CN109628265 A CN 109628265A CN 201910019926 A CN201910019926 A CN 201910019926A CN 109628265 A CN109628265 A CN 109628265A
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- general flavone
- liver
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- SGEWCQFRYRRZDC-VPRICQMDSA-N vitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O SGEWCQFRYRRZDC-VPRICQMDSA-N 0.000 description 1
- PZKISQRTNNHUGF-UHFFFAOYSA-N vitexine Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O PZKISQRTNNHUGF-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of liver-protection health-care molecule wine containing general flavone, break through traditional traditional Chinese medical prescription processing or brewing method, enrichment extraction is carried out to the medicinal and edible plant rich in flavone compound by ferment extraction process and obtains general flavone crude extract fermentation liquid, then general flavone powder is obtained, general flavone powder is configured to molecule wine with white wine in proportion.Molecule wine of the present invention has adjuvant treatment or prevention effect to alcoholic hepatic injury, the content of glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminease (AST) in liver organization supernatant can be reduced, total antioxidant capacity (T-AOC) in liver organization supernatant is increased；Make people while pleasure is drunk in enjoyment, can make liver from or mitigate alcohol injury；Compared with traditional soaked liquor, wine and women-sensual pursuits is light, free from extraneous odour, no precipitated impurities, and long-term drinking can increase body and resist the ability of alcohol and the tolerance to alcohol.
Description
Technical field
The invention belongs to health care molecule wine fields, and in particular to a kind of liver-protection health-care molecule wine containing flavone component.
Background technique
Flavone compound (flavonoids) is that one kind is present in nature, there is C6-C3-C6 to be skeleton, belongs to and planting
Object secondary metabolite.Flavone compound is usually combined into glycoside in plant with sugar, and fraction is with free state (aglycon)
Form exist.All contain flavone compound in most plants, it plant growth, develop, bloom, result with
And antibacterial diseases prevention etc. plays an important role.
Flavone compound is one of main active in medicinal plant, have anti-oxidant, liver protection, anti-inflammatory, antiallergy,
Anti- mattress, anti-mutation, the physiological activity for protecting the wide spectrums such as cardio-cerebrovascular and antiviral and desinsection.Recently as research side
The continuous improvement of method and technology further has found many flavone compounds new type and physiological action again, especially pair
Radicals scavenging and the effect of anti-cancer and cancer-preventing make the research of bioflavonoid enter a new stage.Such compound
It is widely used in medicine, field of food.
Flavone compound has played important function in the prevention and treatment of cardiovascular and cerebrovascular diseases.From the sixties, both at home and abroad
Successively developed included with various ginkgo agents made of ginkgo biloba p.e 24% flavones (Quercetin, different sandlwood
Element, Kaempferol and its glucoside are constituted), it is suitable for brain disorder, intellectual function decline, the adjoint limbs of peripheral vessel circulatory disorders
Thrombosis.Clinically there is good curative effect for treating coronary heart diseases and angina pectoris, cranial vascular disease etc..It is always yellow using sea-buckthorn
The Sindacon (5mg/ piece) of ketone exploitation is the ideal day for treating angina pectoris, prevention of arterial atherosis, myocardial infarction, cerebral thrombosis
Right drug, to treating anginal total effective rate up to 97.1%.Other similar drug is also: using in leaves of Hawthorn Quercetin,
Rutin, Vitexin etc. extract general flavone and " Yixintong " piece, Pueraria Flavonid, pubescens total flavone, radix scrophulariae general flavone, hardship are made
Join general flavone and single ingredient Puerarin (isoflavones carbon glucoside).
Flavone compound as emerging health food active constituent, with it is pure natural, quick be, toxic side effect is low or
The features such as nontoxic are brought true based on the theory and stringent scientific experiment of human body supplement flavones for eater
Health experience.Global flavone compound heat is being risen, and flavone compound health-product market prospect is extremely wide.It is right
It is concentrated mainly on to cardiovascular and cerebrovascular disease in the application of the health-care effect of flavone compound, anti-oxidant equal fields, not yet
It is in adjuvant treatment alcoholic liver injury field product and the research of application.
Heavy drinking can cause acute alcoholism, and chronic alcoholism can cause gastrointestinal reaction, phrenoblabia and Alcoholic
The illnesss such as hepatopathy (Alcoholic liver disease, ALD).ALD is common chronic due to caused by lasting excessive drinking
Hepatopathy, in American-European countries, the main reason for ALD is global incidence and the death rate.The range of disease is scorching from fatty liver to liver
Disease, necrosis, progressive fibrosis and hepatocellular carcinoma.Hepatitis, occurs approximately in greatly 10% to 35% chronic drinking person, and hepatitis is recognized
For reversible lesion can occur during ALD.Up to the present, clinically there are no ideal drug therapy ALD.?
China, as the improvement of people's living standards, more and more people participate in drinking, while the disease incidence of ALD also gradually increases.Cause
This, actively mitigates harm of the alcohol to human body, and prevention and treatment alcohol there is important economic significance and society to anticipate ALD caused by human body
Justice.
Ferment metaplasia object extraction process is a kind of Novel Chinese herbal medicine extraction process.Extracting mode reaction is mild, recovery rate
Height, intermediate product is less, mainly by the effect of microorganism and its enzyme, removal or cellulose in degradation Chinese herbal medicine,
The macromolecular substances such as starch, protein, pectin, at the same convert Extraction and enrichment obtain vitamin, flavonoids, triterpenes, organic acid,
The nutrition such as amino acid, minerals and active function small-molecule substance are easy to subsequent purification processing.Its main feature is that haveing suffered ferment
Organic solvent is not used in biological extraction process.
Traditional health liquor is to be added directly into wine by will be enriched in the Chinese herbal medicine of active constituent by for a long time
It is brewed to form, the drawbacks of this traditional handicraft be can only individual workship's output, because of complicated component multiplicity, quality controllability is poor, nothing
Method large-scale production；Secondly, traditional infusion technology extraction time is long, active constituent recovery rate is lower, causes the wasting of resources cannot
Effective use；It is that flavour of a drug are dense and color is deeper again, is unfavorable for consumer's receiving.
Summary of the invention
The purpose of the present invention is to provide a kind of liver-protection health-care molecule wine containing flavone component has adjuvant treatment or pre-
Anti- alcoholic liver injury effect, long to solve Traditional health care wine infusion technology extraction time, active constituent recovery rate is low, and quality can
The problems such as control property is poor.
In order to achieve the above objectives, the present invention adopts the following technical scheme that:
A kind of liver-protection health-care molecule wine containing general flavone is the molecule wine made of general flavone powder and white wine.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, the alcoholic strength of the white wine is 40~
52vol%.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, general flavone in the general flavone powder
Content is not less than 75%.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, general flavone powder and quality of white spirit volume ratio
Are as follows:
0.2~1.6g/500mL.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, the general flavone powder is that one kind is rich in
The ferment extract of the medicinal and edible plant of flavone compound or a variety of medicinal and edible plants rich in flavone compound
Ferment extract composition.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, the medicine rich in flavone compound
Food homologous plant includes that vine tea, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae, tartary buckwheat are one such
Or it is several.
Preferably, in the liver-protection health-care molecule wine above-mentioned containing general flavone, flavones is rich in for every kind in the composition
The ferment extract of the medicinal and edible plant of class compound it is identical in quality.
The preparation method of liver-protection health-care molecule wine above-mentioned containing general flavone takes one or more total according to required proportion
Flavone powder and white wine are configured to molecule wine, homogeneous, after electromagnetism aging ageing to obtain the final product.
Preferably, in the preparation method of the liver-protection health-care molecule wine above-mentioned containing general flavone, every kind of general flavone
Powder the preparation method comprises the following steps: through crushing and after mixing by every kind of medicinal and edible plant rich in flavone compound, according to
Following mass fraction: 1~5 part of medicinal and edible plant crushed material, 3~20 parts of pure water, 0.5~1 part of glucose, salt 0.3~
It is 0.5 part, 0.003~0.005 part of cellulase, close under conditions of 25~35 DEG C after 0.003~0.005 part of yeast powder mixing
Seal ferment obtains the fermentation liquid that alcoholic strength is 8~12vol%, is filtered using 0.2 μm~0.4 μm of aseptic filtration film, filtrate warp
Be concentrated under reduced pressure, under the conditions of 0~5 DEG C place be precipitated precipitating, collect precipitating and after recrystallizing repeatedly to obtain the final product.
Preferably, in the preparation method of the liver-protection health-care molecule wine above-mentioned containing general flavone, the medicinal and edible plant
Smashed size is 80~100 mesh.
The medicine have the advantages that providing a kind of liver-protection health-care molecule wine containing flavone component, break through in traditional
Medical prescription processes or brewing method, is carried out by ferment extraction process to the medicinal and edible plant rich in flavone compound
Enrichment, which is extracted, obtains general flavone crude extract fermentation liquid, after filtering and removing slag, is concentrated under reduced pressure, and low temperature, which is placed, is precipitated precipitating, collects precipitating
And recrystallize, general flavone powder is obtained, then general flavone powder is configured in proportion with alcoholic strength for the white wine of 40~52vol%
Molecule wine, homogeneous, analytical control is filling, obtains molecule wine after electromagnetism aging ageing.Molecule wine of the invention has adjuvant treatment
Or preventing alcoholic liver injury effect, reduce alcoholic liver injury animal's liver tissue supernatant in glutamic-pyruvic transaminase (ALT) and
The content of glutamic-oxalacetic transaminease (AST) increases the health care molecule wine of total antioxidant capacity (T-AOC) in liver organization supernatant, solution
The problems such as certainly Traditional health care wine infusion technology extraction time is long, and active constituent recovery rate is low, and quality controllability is poor is that one kind is convenient for
Large-scale production, while it being rich in effect substance flavone component, wine and women-sensual pursuits is yellowish, no precipitating, free from extraneous odour, can enjoy pleasure of drinking
While, and can avoid or protect alcohol to the health care molecule wine product of the damage of liver；People can be made to drink pleasure in enjoyment
While, make liver from or mitigate alcohol injury；Compared with traditional soaked liquor, wine and women-sensual pursuits is light, free from extraneous odour, and no precipitating is miscellaneous
Matter, long-term drinking can increase body and resist the ability of alcohol and the tolerance to alcohol.
Detailed description of the invention
Fig. 1 be flower of kudzuvine general flavone molecule wine to alcoholic liver injury mouse liver tissue ALT, AST and T-AOC influence ( N=3)；
Fig. 2 be vine tea general flavone molecule wine to alcoholic liver injury mouse liver tissue ALT, AST and T-AOC influence ( N=3)；
Fig. 3, which is that flower of kudzuvine general flavone molecule wine is morphologic on alcoholic liver injury mouse liver histopathology, influences (400
×,N=3)；
Fig. 4, which is that flower of kudzuvine general flavone molecule wine is morphologic on alcoholic liver injury mouse liver histopathology, influences (400
×,N=3).
Specific embodiment
Embodiment 1
A kind of liver-protection health-care molecule wine containing general flavone, is the molecule wine made of general flavone powder and white wine.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps: taking general flavone powder and white wine to prepare ingredient according to required proportion
Sub- wine, homogeneous, after electromagnetism aging ageing to obtain the final product.
Embodiment 2
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 52vol% according to 1.5g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is the ferment extract of flower of kudzuvine.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Flower of kudzuvine is crushed as 80 mesh, according to following mass fraction: 1 part of flower of kudzuvine crushed material, 3 parts of pure water, glucose 1
Part, it 0.3 part of salt, 0.005 part of cellulase, is sealed by fermentation under conditions of 25 DEG C after 0.003 part of yeast powder mixing and obtains wine
Precision is the fermentation liquid of 8vol%, is then filtered using 0.4 μm of aseptic filtration film, filtered fermentation liquid is concentrated under reduced pressure,
Precipitating is precipitated in 0 DEG C of placement, collects and precipitates and recrystallize repeatedly, obtain flower of kudzuvine general flavone powder；Finally, by flower of kudzuvine general flavone powder
The mass volume ratio of 1.5g/500mL and 52vol% white wine are configured to molecule wine in proportion at end, and homogeneous, analytical control is filling, electricity
Molecule wine is obtained after magnetic ageing ageing.
Embodiment 3
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 52vol% according to 1.5g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is the ferment extract of vine tea, and wherein the content of general flavone is
75%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Vine tea is crushed as 80 mesh, according to following mass fraction: 3 parts of vine tea crushed material, 10 parts of pure water, glucose
It 0.7 part, 0.4 part of salt, 0.004 part of cellulase, is sealed by fermentation and obtains under conditions of 25 DEG C after 0.004 part of yeast powder mixing
Alcoholic strength is the fermentation liquid of 10vol%, then spare using 0.2 μm of aseptic filtration film filtering, and filtered fermentation liquid is subtracted
Pressure concentration is precipitated precipitating in 0 DEG C of placement, collects and precipitate and recrystallize repeatedly, obtain vine tea general flavone powder；Finally, by vine tea
The mass volume ratio of 1.6g/500mL and 52vol% white wine are configured to molecule wine, homogeneous, analysis inspection to general flavone powder in proportion
It tests, it is filling, molecule wine is obtained after electromagnetism aging ageing.
Embodiment 4
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 40vol% according to 0.2g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is the ferment extract of Rosa roxburghii Tratt, ginkgo leaf and pueraria lobata, wherein
The content of general flavone is 95%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Rosa roxburghii Tratt, ginkgo leaf and pueraria lobata are crushed and is 100 mesh and is uniformly mixed, according to following mass fraction: Rosa roxburghii Tratt, ginkgo leaf
It is respectively 1 part, 1 part and 3 parts with pueraria lobata crushed material, 20 parts of pure water, 0.5 part of glucose, 0.5 part of salt, cellulase 0.003
Part, it is sealed by fermentation under conditions of 35 DEG C after 0.005 part of yeast powder mixing and obtains the fermentation liquid that alcoholic strength is 12vol%, then
It is spare using 0.3 μm of aseptic filtration film filtering, filtered fermentation liquid is concentrated under reduced pressure, precipitating is precipitated in 5 DEG C of placements, collects
It precipitates and recrystallizes repeatedly, obtain Rosa roxburghii Tratt, ginkgo leaf and Pueraria Flavonid powder；Finally, Rosa roxburghii Tratt, ginkgo leaf and pueraria lobata is always yellow
The mass volume ratio of 0.2g/500mL and 40vol% white wine are configured to molecule wine, homogeneous to ketone powder in proportion, and analytical control fills
Dress, obtains molecule wine after electromagnetism aging ageing.
Embodiment 5
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 45vol% according to 0.6g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is the ferment of stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat
Change extract, wherein the content of general flavone is 85%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat are crushed and is 90 mesh and is uniformly mixed, according to following mass parts
Number: stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat powder mince each 1 part, and 15 parts of pure water, 0.8 part of glucose, salt 0.4
Part, it 0.005 part of cellulase, is sealed by fermentation under conditions of 30 DEG C after 0.004 part of yeast powder mixing and obtains alcoholic strength and be
The fermentation liquid of 10vol%, it is then spare using 0.2 μm of aseptic filtration film filtering, filtered fermentation liquid is concentrated under reduced pressure,
Precipitating is precipitated in 2 DEG C of placements, collects and precipitates and recrystallize repeatedly, it is always yellow to obtain stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat
Ketone powder；Finally, by the quality of stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat general flavone powder 0.6g/500mL in proportion
Volume ratio and 45vol% white wine are configured to molecule wine, and homogeneous, analytical control is filling, obtains molecule wine after electromagnetism aging ageing.
Embodiment 6
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 50vol% according to 0.8g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is the ferment extract of vine tea, pueraria lobata, radix scutellariae and tartary buckwheat,
Wherein the content of general flavone is 80%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Vine tea, pueraria lobata, radix scutellariae and tartary buckwheat are crushed and is 80 mesh and is uniformly mixed, according to following mass fraction: vine tea, Pueraria lobota
Root, radix scutellariae and tartary buckwheat powder mince respectively 0.5 part, 1 part, 0.3 part and 0, and 2 parts, 7 parts of pure water, 0.6 part of glucose, salt
It 0.5 part, 0.003 part of cellulase, is sealed by fermentation under conditions of 25 DEG C after 0.005 part of yeast powder mixing and obtains alcoholic strength and be
The fermentation liquid of 8vol%, it is then spare using 0.4 μm of aseptic filtration film filtering, filtered fermentation liquid is concentrated under reduced pressure, 3
DEG C place be precipitated precipitating, collect precipitate simultaneously recrystallizes repeatedly, obtain vine tea, pueraria lobata, radix scutellariae and tartary buckwheat general flavone powder；Most
Afterwards, by vine tea, pueraria lobata, radix scutellariae and tartary buckwheat general flavone powder, the mass volume ratio of 0.8g/500mL and 50vol% are white in proportion
Wine is configured to molecule wine, and homogeneous, analytical control is filling, obtains molecule wine after electromagnetism aging ageing.
Embodiment 6
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 42vol% according to 1g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is ginkgo leaf, flower of kudzuvine, stauntonvine, feed with paper-mulberry leaf, radix scutellariae and bitter buckwheat
The ferment extract of wheat, wherein the content of general flavone is 90%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
Ginkgo leaf, flower of kudzuvine, stauntonvine, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat are crushed as 100 mesh and be uniformly mixed, according to
Following mass fraction: ginkgo leaf, flower of kudzuvine, stauntonvine, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat powder mince each 0.5 part, and 5 parts of pure water, Portugal
0.8 portion of grape sugar, 0.005 part of cellulase, is sealed by fermentation under conditions of 35 DEG C after 0.003 part of yeast powder mixing by 0.3 part of salt
The fermentation liquid that alcoholic strength is 12vol% is obtained, it is then spare using 0.2 μm of aseptic filtration film filtering, by filtered fermentation
Liquid is concentrated under reduced pressure, and precipitating is precipitated in 5 DEG C of placements, collects and precipitates and recrystallize repeatedly, obtain ginkgo leaf, flower of kudzuvine, stauntonvine, paper mulberry
Leaf, radix scutellariae and tartary buckwheat general flavone powder；Finally, by ginkgo leaf, flower of kudzuvine, stauntonvine, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat general flavone
The mass volume ratio of 1g/500mL and 42vol% white wine are configured to molecule wine to powder in proportion, and homogeneous, analytical control is filling, electricity
Molecule wine is obtained after magnetic ageing ageing.
Embodiment 7
A kind of liver-protection health-care molecule wine containing general flavone, is by the white wine of general flavone powder and 52vol% according to 1.2g/
Molecule wine made of the proportion of 500mL, wherein general flavone powder is vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sand
Spine, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat ferment extract, wherein the content of general flavone be 75%.
Above-mentioned liver-protection health-care molecule wine the preparation method comprises the following steps:
It is 80 that vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat, which are crushed,
Mesh is simultaneously uniformly mixed, according to following mass fraction: vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf,
Radix scutellariae and tartary buckwheat powder mince each 0.5 part, and 18 parts of pure water, 1 part of glucose, 0.5 part of salt, 0.004 part of cellulase, yeast
It is sealed by fermentation under conditions of 25 DEG C after 0.005 part of powder mixing and obtains the fermentation liquid that alcoholic strength is 12vol%, then use 0.2 μ
The aseptic filtration film filtering of m is spare, and filtered fermentation liquid is concentrated under reduced pressure, and precipitating is precipitated in 0 DEG C of placement, collection precipitating is simultaneously anti-
Multiple recrystallization, obtains vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat general flavone
Powder；Finally, by vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae and tartary buckwheat general flavone
The mass volume ratio of 1.2g/500mL and 52vol% white wine are configured to molecule wine to powder in proportion, and homogeneous, analytical control is filling,
Molecule wine is obtained after electromagnetism aging ageing.
In order to further verify the purpose of the present invention and beneficial effect, inventor has carried out following experiment:
1. experimental animal
Male mice in kunming, 18~22g of weight rise prosperous Bill experimental animal sale Co., Ltd by Chongqing and provide (conjunction
Lattice card SCXK (army) 2012-0011).Animal free water, feed, 23 ± 2 DEG C of feeding environment temperature, humidity 60 ± 10%,
Pellet is risen prosperous Bill experimental animal sale Co., Ltd by Chongqing and is provided.
2. experimental drug and reagent
Molecule wine containing general flavone: using flower of kudzuvine general flavone molecule wine and vine tea made from above-described embodiment 2 and embodiment 3
General flavone molecule wine.
Practise wine: in Kweiyang supermarket, Wal-Mart (lot number are as follows: 750813602S), alcoholic strength is 52 degree for purchase.
BCA determination of protein concentration kit: the green skies biotechnology research institute in Jiangsu；T-AOC: bioengineering is built up in Nanjing
Research institute；ALT: Bioengineering Research Institute is built up in Nanjing；AST: Bioengineering Research Institute is built up in Nanjing.
3. experimental group and administration
Kunming mouse is randomly divided into 5 groups (every group 10), blank control group；Model group (0.15mL/20g) and flavones
Molecule wine is basic, normal, high (1.0mg/0.15mL/20g, 1.5mg/0.15mL/20g and 2.0mg/0.15mL/20g), daily stomach-filling 1
It is secondary, continuous 21 days.Routinely claim a weight during test weekly.
4.HE dyeing
Cervical dislocation execution is carried out in the mouse of last stomach-filling, takes mouse lobus dexter liver organization to be cut into 1 × 1cm, is put into 4%
Paraformaldehyde it is fixed for 24 hours, 4 μm of serial section after paraffin embedding carry out HE dyeing, the above-mentioned piece cut are put in 60 DEG C of baking ovens
In dry, then carry out experimental implementation below.(1) dimethylbenzene dewaxes: dimethylbenzene (I) 10 minutes → dimethylbenzene (II) 10 minutes
5 minutes → 95% ethyl alcohol of → 100% ethyl alcohol, 5 minutes → 80% ethyl alcohol, 5 minutes → distillation washing 5 minutes；(2) haematoxylin, Yihong
Dyeing: after dewaxing → haematoxylin solution dyes 5 minutes → distills 10 seconds → acidic alcohol of washing and breaks up the punching of 2 seconds → tap water
10 minutes → distilled water immersion, 2 minutes → eosin stains, 3 minutes → tap water is washed to rinse 3 minutes → distilled water immersion 10 seconds；(3)
Dehydration, transparent, mounting: 80% ethyl alcohol, 30 seconds → 95% ethyl alcohol, 30 seconds → 100% ethyl alcohol (I), 1 minute → 100% ethyl alcohol (II) 1
Minute → dimethylbenzene (I) 2 minutes → dimethylbenzene (II) 2 minutes → neutral gum mounting；(4) BX43 is just setting micro- sem observation liver
The pathologic condition of tissue, and photograph to record.
5. the measurement of liver organization T-AOC, ALT and AST
Mouse after gastric infusion carries out cervical dislocation execution, and liver organization is taken to weigh weight, according to weight (g):
Volume (mL)=1:9 ratio, is added the physiological saline of 9 times of volumes, and mechanical homogenisation under the conditions of ice-water bath, is centrifuged by 2500 revs/min
10min takes supernatant to be measured.
6. statistical method application SPSS13.0 statistical software is analyzed, as a result with meanIt indicates, mean between group
Compare and is examined using t.
7. result
7.1 flower of kudzuvine general flavone molecule wine and vine tea general flavone molecule wine are respectively to alcoholic liver injury mouse general state
It influences
Blank group: mouse hair is shinny, and drinking-water, feed and stool are normal, movable sensitive, no death；
Model group: mouse gavage practise occur after drinking outlet difficulty, dignity it is dull, apathetic, appetite stimulator drinks water
Phenomena such as amount is reduced, drowsiness and movable slow, gavaging habit, 30min or so enters Somnolence after drinking, revives within 4 hours or so, 6 is small
When or so it is completely awake, and it is poor to show as hair luster degree, defecates with completely mashed.
Flower of kudzuvine general flavone molecule wine and vine tea general flavone molecule wine administration group (low, in and high dose): the appetite ratio of mouse
Model group is good, and the time of sobering up is more early than model group.
7.2 flower of kudzuvine general flavone molecule wine and vine tea general flavone molecule wine are respectively to the shadow of alcoholic liver injury mouse death rate
It rings
In experimentation, blank group is without dead mouse.Preliminary experiment early period is (0.15mL/ to vina amount to find out suitably
20g), the flower of kudzuvine general flavone molecule wine model group death rate is respectively 50%；There are 5 death, middle dosage in administration low dose group (D)
Group (Z) has 4 death, and high dose (G) has 2 death, and the death rate is respectively 50%, 40% and 20%.Vine tea general flavone molecule
The wine model group death rate is respectively 60%；There are 5 death in administration low dose group (D), middle dose group (Z) has 3 death, high agent
Amount (G) has 2 death, and the death rate is respectively 50%, 30% and 20%.Dead mouse is discovery in second day after stomach-filling, solution
Cuing open not discovery thoracic cavity has the phenomenon that extravasated blood or gastric perforation, but finds that there is a large amount of liquid in stomach the inside and big enteral has a large amount of gas
Body exists without food.Phenomena such as performance is walked haltingly before dead, is twitched, irritated and belly flatulence.Table 1 refers to flower of kudzuvine general flavone
Influence of the molecule wine to alcoholic liver injury mouse death rate；Table 2 refers to vine tea general flavone molecule wine to alcoholic liver injury mouse
The influence of the death rate.
Influence of the 1 flower of kudzuvine general flavone molecule wine of table to alcoholic liver injury mouse death rate
Note: K: blank control group；M: vina amount 0.15mL/20g group is practised；D:1.0mg/0.15mL/20g；
Z:1.5mg/0.15mL/20g group；G:2.0mg/0.15mL/20g.
Influence of the 2 vine tea general flavone molecule wine of table to alcoholic liver injury mouse death rate
Note: K: blank control group；M: vina amount 0.15mL/20g group is practised；D:1.0mg/0.15mL/20g；
Z:1.5mg/0.15mL/20g group；G:2.0mg/0.15mL/20g.
7.3 flower of kudzuvine general flavones and vine tea general flavone molecule wine are respectively to alcoholic liver injury mouse liver tissue ALT, AST
With the influence of T-AOC
As depicted in figs. 1 and 2, compared with blank control group (K), the level of ALT and AST is significant in model group liver organization
Increase and the ability of T-AOC obviously weakens (* P ﹤ 0.05).Compared with model group, each dosage group liver organization of general flavone molecule wine
The horizontal decrease of middle ALT and AST and the ability of T-AOC increase, and have statistical difference (#P ﹤ 0.05).Illustrate that flower of kudzuvine is always yellow
Ketone molecule wine and vine tea general flavone molecule wine have the function of reducing liver organization transaminase and increase total antioxidant capacity.
7.4 flower of kudzuvine general flavones and vine tea general flavone molecule wine are respectively to alcoholic liver injury mouse liver histopathology form
Influence
As shown in Figures 3 and 4, blank control group mouse hepatic tissue structure is normal, liver cell marshalling, clear in structure,
Without oedema phenomenon and inflammatory cell.Compared with blank control group, model group murine liver tissue structure and liver cell are disorganized, more
Oedema phenomenons are presented in number liver cells, inflammatory cell infiltration in hepatic tissue, liver cell fibrosis, have rouge not of uniform size in hepatic tissue
Fat drips vacuole.Compared with model group, molecule wine administration group disease states mitigate.Low dosage and middle dosage administration group murine liver tissue
Structure and liver cell arrangement are still neat, and inflammatory cell and fat drop vacuole subtract compared with model group in liver cell oedema phenomenon, hepatic tissue
Gently.High dose administration group murine liver tissue is clear in structure, and liver cell marshalling, size are uniform, and inflammatory cell and fat drop are empty
Compared with model group group, symptom significantly mitigates bubble.
This experiment the result shows that flower of kudzuvine general flavone and vine tea general flavone molecule wine can reduce alcoholic liver injury animal
The death rate, reduce liver organization in ALT and AST level and increase T-AOC vigor.See in pathological section result containing
The molecule wine of general flavone can make hepatic tissue clear in structure, and liver cell marshalling, size are uniform, and inflammatory cell is reduced.
Claims (10)
1. a kind of liver-protection health-care molecule wine containing general flavone, which is characterized in that divided made of general flavone powder and white wine
Sub- wine.
2. the liver-protection health-care molecule wine according to claim 1 containing general flavone, which is characterized in that the alcohol of the white wine
Degree is 40~52vol%.
3. the liver-protection health-care molecule wine according to claim 1 containing general flavone, which is characterized in that the general flavone powder
The content of general flavone is not less than 75% in end.
4. the liver-protection health-care molecule wine according to claim 1 containing general flavone, which is characterized in that general flavone powder with it is white
Quality of Liquors volume ratio are as follows: 0.2~1.6g/500mL.
5. the liver-protection health-care molecule wine according to any one of claims 1 to 4 containing general flavone, which is characterized in that described
General flavone powder is a kind of ferment extract or a variety of rich in flavonoids of medicinal and edible plant rich in flavone compound
The composition of the ferment extract of the medicinal and edible plant of compound.
6. the liver-protection health-care molecule wine according to claim 5 containing general flavone, which is characterized in that described is rich in flavones
The medicinal and edible plant of class compound include vine tea, Rosa roxburghii Tratt, ginkgo leaf, pueraria lobata, flower of kudzuvine, stauntonvine, sea-buckthorn, feed with paper-mulberry leaf, radix scutellariae,
Tartary buckwheat is one such or several.
7. the liver-protection health-care molecule wine according to claim 5 containing general flavone, which is characterized in that in the composition
Every kind rich in flavone compound medicinal and edible plant ferment extract it is identical in quality.
8. the preparation method of any liver-protection health-care molecule wine containing general flavone of claim 1~7, which is characterized in that
It takes one or more general flavone powder according to required proportion and white wine is configured to molecule wine, homogeneous, after electromagnetism aging ageing to obtain the final product.
9. the preparation method of the liver-protection health-care molecule wine according to claim 6 containing general flavone, which is characterized in that described
Every kind of general flavone powder the preparation method comprises the following steps: being crushed and being mixed every kind of medicinal and edible plant rich in flavone compound
After uniformly, according to following mass fraction: 1~5 part of medicinal and edible plant crushed material, 3~20 parts of pure water, glucose 0.5~1
Part, 0.3~0.5 part of salt, 0.003~0.005 part of cellulase, after 0.003~0.005 part of yeast powder mixing, 25~35
It is sealed by fermentation under conditions of DEG C and obtains the fermentation liquid that alcoholic strength is 8~12vol%, then use 0.2 μm~0.4 μm of degerming
Membrane filtration, filtrate through being concentrated under reduced pressure, under the conditions of 0~5 DEG C place be precipitated precipitating, collect precipitating and after recrystallizing repeatedly i.e.
?.
10. the preparation method of the liver-protection health-care molecule wine according to claim 7 containing general flavone, which is characterized in that institute
Stating the smashed size of medicinal and edible plant is 80~100 mesh.
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Application publication date: 20190416 |