CN108391813A - A kind of relieving alcoholism and protecting liver ferment and preparation method thereof - Google Patents
A kind of relieving alcoholism and protecting liver ferment and preparation method thereof Download PDFInfo
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- CN108391813A CN108391813A CN201810282461.0A CN201810282461A CN108391813A CN 108391813 A CN108391813 A CN 108391813A CN 201810282461 A CN201810282461 A CN 201810282461A CN 108391813 A CN108391813 A CN 108391813A
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of relieving alcoholism and protecting liver ferment, are prepared with integration of drinking and medicinal herbs raw material；A kind of preparation method of relieving alcoholism and protecting liver ferment, two step enzymatic isolation methods extraction integration of drinking and medicinal herbs raw material obtain enzymolysis liquid, enzymolysis liquid and water are mixed, add auxiliary material, beneficial flora is accessed after sterilization treatment and carries out multi-stage combination fermentation, subsequently enters the ageing stage, supernatant is finally collected by centrifugation.After testing, Puerarin, amino acid content in prepared ferment through the invention, ferment oxidation resistance etc. and relieving alcoholism and protecting liver index of correlation, it is above in conventional high-temperature processing procedure aqueous solution and corresponds to index, therefore it uses the relieving alcoholism and protecting liver ferment prepared by the method for the present invention in acceleration tricarboxylic acid cycle and promotes the decomposition of acetaldehyde, improve ethanol in blood and triglyceride concentration, improving oxidation resistance of hepatic tissue etc. has important potential, and hepatic injury and the exploitation of other ferment products for caused by alleviating people's excessive drinking have important directive significance.
Description
Technical field
The invention belongs to food technology fields, and in particular to a kind of relieving alcoholism and protecting liver ferment and preparation method thereof.
Background technology
Pueraria lobata is yellow containing flavone component Puerarin, Puerarin xyloside, daizeol, soybean as integration of drinking and medicinal herbs raw material
Ketone glucoside and cupreol, arachidic acid etc., then contain in Radix Salviae Miltiorrhizae root master containing fat-soluble Diterpenoids from bulbus and water-soluble phenolic acid at
Point, also contain flavonoids, triterpenes, the other compositions such as sterol；The two is all nutritive value height, the medicine with extensive bioactivity
Eat homologous sex-health products.A variety of amino acid, vitamin, organic acid, inorganic salts and the enzyme to boost metabolism are rich in honey
Class is important functional product.
Excessive drinking be easy liver is caused to damage, though have more relieving alcoholism and protecting liver product on the market at present, as drug, in
Medicine preparation and health products etc., although decomposing alcohol concentration can be played to a certain extent, caused by alleviation alcohol concentration is excessively high
The symptoms such as dizzy, vomiting, but drug can cause liver, kidney metabolism to bear and generate different degrees of while relieving the effect of alcohol
Injury.Moreover, integration of drinking and medicinal herbs raw material mostly carries out processing extraction using traditional high temperature concocting method at present, although this method is simple
Just, but cannot the active constituent in raw material be effectively subjected to complete extraction and release, and high temperature impregnates and digesting technoloy holds
Some active skull cap components of destructible.By investigation, it there is no using integration of drinking and medicinal herbs raw material as raw material and pass through completely on the market at present
Probiotics fermention converts the relieving alcoholism and protecting liver Related product to be formed.
Invention content
In order to solve the above technical problem, the present invention provides a kind of relieving alcoholism and protecting liver ferment and preparation method thereof；The present invention with
Pueraria lobata, Poria cocos, hawthorn etc. reported with the relevant integration of drinking and medicinal herbs raw material of relieving alcoholism and protecting liver, through biological enzymolysis and probiotics fermention,
Functional relieving alcoholism and protecting liver ferment is re-dubbed after after-ripening so that each ingredient synergistic effect helps to alleviate excessive drinking to liver
Caused by damage.
The present invention is achieved through the following technical solutions, and the present invention provides a kind of relieving alcoholism and protecting liver ferment, same with medicine food
Source raw material is prepared, and integration of drinking and medicinal herbs raw material is grouped as by the group of following parts by weight：1-2 parts of flower of kudzuvine, 3-5 parts of pueraria lobata, Chinese yam 1-3
Part, 1-2 parts of Herba portulacae, 3-4 parts of dark plum, 2-3 parts of sealwort, 1-2 parts of dandelion, 1-2 parts of honeysuckle, 1-3 parts of fructus amomi, almond 1-2
Part, 2-3 parts of cassia seed, 1-2 parts of the coptis, 1-3 parts of Poria cocos, 2-3 parts of Rhizoma Atractylodis Macrocephalae, 1-3 parts of Radix Salviae Miltiorrhizae, 1-2 parts of dried orange peel, 2-4 parts of hawthorn,
1-2 parts of hoveniae semoveniae semen.
Pueraria lobata has pueraria isoflavone and Puerarin：Brain and the effect of coronal vascular flow amount can be increased, also with low vascular resistence
With Green Tea Extract and improve SOD active functions.Inhibit ethyl alcohol stomach absorb and with accelerating alcohol katabolism, to reduce body blood
Determining alcohol, to play relieving alcoholism and preventing drunkenness effect；There is hoveniae semoveniae semen Central nervous depressant, anti peroxidation of lipid and blood pressure lowering to make
With；Flower of kudzuvine can be effectively improved Ethanol concentration in rat blood, reduce by three fat of liver oil in blood, improve the oxidation resistance of hepatic tissue, promote fat
Fat acid oxidase decomposes, and reduces lipid level；Chinese yam, fructus amomi, almond hydrolysis generation glucose can inhibit alcohol dehydrogenase, slow down second
The generation of aldehyde；Herba portulacae contains Herba portulacae polysaccharide, contains the ingredients such as gallic acid, citric acid, malic acid, the above ingredient in dark plum
With antiviral, oxidation resistant effect, the oxidation resistance of body can be improved, accelerates acetal dehyde decomposition；Studies have shown that sealwort carries
Take object and dandelion extract that there is obvious protective function to acute hepatic injury mice；Main chemical compositions have flavones in honeysuckle
Class, organic acid, glycoside and volatile oil etc..Mainly there is with the effect of honeysuckle hepatic cholagogic triterpenoid saponins wherein in glycoside
It closes；Cassia seed extracting solution has an impact rat liver CYP450 enzymes enzymatic activity, mRNA and protein expression level, has and reduces liver
The effect of lipid precipitation；The coptis damages the protective effect having to liver mitochondrion caused by insulin resistance rat oxygen radical；
Poria cocos, Rhizoma Atractylodis Macrocephalae, hawthorn have detoxication to hepatic injury；Radix Salviae Miltiorrhizae can promote microcirculation, inhibit liver cell damage and death,
Improve intracellular anaerobic environment, promote liver cell synthesis and secretion, there is obvious protective function to liver.
Another aspect of the present invention provides a kind of preparation method of relieving alcoholism and protecting liver ferment, and it is former that two step enzymatic isolation methods extract integration of drinking and medicinal herbs
Expect to obtain enzymolysis liquid, enzymolysis liquid and water are mixed, add auxiliary material, access beneficial flora carries out multi-stage combination fermentation after sterilization treatment,
The ageing stage is subsequently entered, supernatant is collected by centrifugation after the completion of conversion；
The two step enzymatic isolation methods of the present invention pass through biology extraction and microorganism conversion work compared with traditional high temperature processing procedure
Active skull cap components in integration of drinking and medicinal herbs raw material are not only completely retained and are discharged, and convert part macromolecular components to by skill
Conducive to the small molecule ingredient of absorption of human body and metabolism, while active secondary metabolites are generated, improved living in relieving alcoholism and protecting liver ferment
The type and function of property ingredient.Two step enzyme methods there is no report in ferment technique.
Ferment fermentation is mostly a kind of bacterium single fermentation in presently disclosed patent, preferably at most the mixing hair of yeast and lactic acid
Ferment, this patent use the multi-stage combination zymotechnique, the advantages of multi-stage combination zymotechnique to be：Multi-stage combination fermentation can be further
Active constituent in release and conversion integration of drinking and medicinal herbs raw material, while different types of abundant prebiotic stimulation metabolite is generated, increase
The type and content of active constituent in strong enzyme.
After Enzymatic Extraction and probiotics fermention, puerarin content processes Puerarin in aqueous solution relative to conventional high-temperature and carries
It is nearly 7 times high.Puerarin has reduction vascular resistence, inhibits the absorption of ethyl alcohol stomach and accelerating alcohol katabolism, to reduce body
Blood alcohol concentration reduces endotoxin (LPS) concentration and Cytochrome P450 2E1 (CYP2E1) is inhibited to express, and reduces free radical and generates,
To mitigate hepatic injury caused by oxidative stress, reach relieving alcoholism and protecting liver effect.
It is detected, sends out to the ferment product and through extracting the amino acid content in solution made from high temperature concocting method
It is existing, by the amino acid content of relieving alcoholism and protecting liver ferment made from this method nearly 10 are improved compared with amino acid content in conventional method
Times.Body metabolic disorder caused by excessively intake alcohol is adjusted in the increase of amino acid content, and the oxidation in stable cytosol is also
Former current potential accelerates tricarboxylic acid cycle, promotes the decomposition of acetaldehyde, reduce the damage of hepatic tissue.
The free radical scavenging activity curve that aqueous solution is processed to the ferment product and conventional high-temperature is detected, relieving alcoholism and protecting liver ferment
The free radical scavenging activity of element improves 92% compared with than tradition processing aqueous solution, has higher oxidation resistance.Alcohol is thin to liver
The toxicity of born of the same parents can make the lipid components excessive oxidation on liver plasma membrane surface, destroy liver cell by the metabolism of influence liver cell
Film.And internal superoxide dismutase, malonaldehyde, glutathione peroxidase, glutathione occur bright after excessive drinking
Aobvious to change, body, which generates a large amount of oxygen radical, makes oxidation reaction enhance, and generates a large amount of free radicals, very to the murder by poisoning of body tissue
Greatly.Therefore, the oxidation resistance of research relieving alcoholism and protecting liver ferment has important directive significance to liver protecting.
Preferably, enzymolysis liquid and water are mixed by the component of following parts by weight：30-45 parts of enzymolysis liquid, 55-70 parts of water.
Preferably, the relieving alcoholism and protecting liver ferment 3000-4000rpm centrifuges 5-10min, collects supernatant；
Preferably, the auxiliary material of addition press enzymolysis liquid and water mixed system parts by weight percentages, for it is following any one
Or arbitrary two kinds and two or more mixing：
Edible glucose 3-8%
Edible sucrose 2-5%
Food yeast powder 1-2%.
Addition auxiliary material be in order to allow microorganism preferably grow and ferment, according to microorganism grow primary demand C the sources N
It is added, additive amount is can to make the range that microorganism preferably grows by experimental demonstration.
Preferably, in supernatant be added with supernatant by weight percentage it is following any one or it is arbitrary two kinds and
Two or more mixtures：
Honey 3-6%
Antierythrite 0.5-1%
Oligofructose 0.5-1%
In order to increase the mouthfeel of relieving alcoholism and protecting liver ferment, mentioned component is added, above-mentioned additive amount is by tasting binding function
What the addition range of sugared effect finally determined.
Preferably, the beneficial flora be prebiotic bacillus amyloliquefaciens, bacillus licheniformis, bacillus amyloliquefaciens,
Bafillus natto, bacillus coagulans, bacillus cereus, Rhizopus oryzae, acetic acid bacteria, mucor, the wine brewing of cloth Laplace saccharomycete
Saccharomycete, utilizing flavoring yeast, Wei Shi yeast, Hansenula anomala, schizosaccharomyces pombe, glutinous rhodotorula, pichia farinose, production
Glycerin candida, candida utili, lactobacillus plantarum, Lactobacillus casei, streptococcus thermophilus, lactobacillus gasseri, bifid bar
Bacterium, staphylococcus xylosus, lactobacillus acidophilus, curls arbitrary two kinds in lactobacillus and two or more mixed at calf staphylococcus
It closes.
Preferably, the beneficial flora access amount is accessed according to the 2-10% of enzymolysis liquid volume.Preferably, the probiotics
Group's access amount is accessed according to the 2-5% of enzymolysis liquid volume.2%-10% is allowed inoculation range；2%-5% is conventional optimal
Inoculation range.
Preferably, multi-stage combination fermentation be arbitrary two kinds in aerobic fermentation, amphimicrobian fermentation, anaerobic fermentation and
Two or more mixed fermentation modes.
Preferably, multi-stage combination fermentation be 30-37 DEG C, 150-200rpm aerobic fermentations 1-2 days, 25-37 DEG C of 50-
100rpm amphimicrobians ferment 1-5 days, 20-37 DEG C standing for fermentation 1-14 days in arbitrary two kinds and two or more mixing send out
Ferment technique.
Preferably, the time in ageing stage is 1-6 months, and temperature is 10-25 DEG C.This is the normal of current ferment after-ripening
Use the time.
Preferably, the specific steps of two step enzymatic isolation methods extraction integration of drinking and medicinal herbs raw material include：
S101：Water is added after integration of drinking and medicinal herbs raw material is crushed, mixed liquor carries out broken wall, the agitated carry out first step of shell-broken liquid
Enzymolysis and extraction；
S102：By the mixed liquor after first step enzymolysis and extraction, stirring carries out second step enzymolysis and extraction.
For integration of drinking and medicinal herbs raw material mostly using traditional high temperature concocting method processing extraction, the present invention uses two step enzyme methods at present,
There are no be reported in the preparation process of ferment for two step enzyme methods；It can pass through the association of variety classes enzyme using two step enzymatic isolation methods
Same-action fully digests and releases the active ingredient in integration of drinking and medicinal herbs raw material, the active ingredient in integration of drinking and medicinal herbs raw material is made fully to release
It is put into enzymolysis liquid, puerarin content processes Puerarin in aqueous solution relative to conventional high-temperature and improves nearly 7 times.Amino acid content
Nearly 10 times are improved compared with amino acid content in conventional method.
Preferably, in S101, broken wall condition is including but not limited to 30-40 DEG C of ultrasound 60-90min, sporoderm-broken rate 85-95%.
Preferably, in S101, the extracting method of first step enzymolysis and extraction：40-60 DEG C, 150-200rpm stirrings, pH 3.5-
5.5, extraction time 60-90min；In S102, the extracting method of second step enzymolysis and extraction：50-70 DEG C, 100-200rpm stirrings,
PH 7.0-8.5, extraction time 30-60min.
Preferably, in S101, enzyme used in first step enzymolysis and extraction is following arbitrary in terms of weight permillage with mixed liquor
Two kinds and two or more mixing：
Preferably, in S102, enzyme used in second step enzymolysis and extraction is following arbitrary in terms of weight permillage with mixed liquor
A kind of or arbitrary two kinds and two or more mixing：
Enzyme used in first step enzymolysis and extraction is acidicenzym, and enzyme used in second step enzymolysis and extraction is neutral or alkalinity
Enzyme fully digests and releases the active ingredient in integration of drinking and medicinal herbs raw material by the synergistic effect of variety classes enzyme.
By puerarin content, amino acid content isoreactivity component content in the relieving alcoholism and protecting liver ferment of the invention prepared and resist
Oxidability is above in conventional high-temperature processing procedure aqueous solution related content and index, therefore using prepared by the method for the present invention
Relieving alcoholism and protecting liver ferment accelerating tricarboxylic acid cycle and promoting the decomposition of acetaldehyde, improve ethanol in blood and triglyceride concentration,
The oxidation resistance etc. for improving hepatic tissue has important potential, for caused by alleviating people's excessive drinking hepatic injury and its
He has important directive significance at the exploitation of ferment product.
Beneficial effects of the present invention：
1) present invention, not only will be in integration of drinking and medicinal herbs raw material by mild and efficient biology extraction and microbial conversion process
Active skull cap components completely retain and discharge, and convert part macromolecular components to small point conducive to absorption of human body and metabolism
Subconstiuent, while active secondary metabolites are generated, improve the type and function of active constituent in relieving alcoholism and protecting liver ferment.
2) present invention has the integration of drinking and medicinal herbs raw material of sober-up function with pueraria lobata, Poria cocos, hawthorn etc., passes through complex enzyme zymohydrolysis method
The active ingredient in integration of drinking and medicinal herbs is extracted, extraction conditions are mild, recovery rate is high, by the bioconversion of probiotics, preferable
While retaining and discharge nutritional ingredient in integration of drinking and medicinal herbs, and include active probiotic and its beneficial metabolic product.
3) it is with the integration of drinking and medicinal herbs raw material with sober-up function, and through biological enzymolysis and prebiotic that the present invention, which prepares products obtained therefrom,
Bacterium converts, fermentation after-ripening and the functional relieving alcoholism and protecting liver ferment compounded.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it is therefore apparent that the accompanying drawings in the following description is only this reality
Without creative efforts, may be used also for those of ordinary skill in the art with some novel embodiments
With obtain other attached drawings according to these attached drawings.
Fig. 1 relieving alcoholism and protecting livers ferment and high temperature process Puerarin phase chromatogram in aqueous solution；
Fig. 2 relieving alcoholism and protecting liver ferment free radical scavenging activity curves；
Fig. 3 high temperature processes aqueous free radical clearance rate curve.
Specific implementation mode
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As background technology part is introduced, though there is more relieving alcoholism and protecting liver product on the market, such as drug, Chinese medicine preparation
And health products etc., although decomposing alcohol concentration can be played to a certain extent, alleviate alcohol concentration it is excessively high caused by it is dizzy, vomit
The symptoms such as spit, but drug can cause liver, kidney metabolism to bear and generate different degrees of injury while relieving the effect of alcohol.
Meanwhile by investigation, there is no to be formed using integration of drinking and medicinal herbs raw material as raw material and converting by probiotics fermention completely at present on the market
Relieving alcoholism and protecting liver Related product.
A kind of relieving alcoholism and protecting liver ferment of the present embodiment, is prepared, integration of drinking and medicinal herbs raw material is by following with integration of drinking and medicinal herbs raw material
The group of parts by weight is grouped as：1-2 parts of flower of kudzuvine, 3-5 parts of pueraria lobata, 1-3 parts of Chinese yam, 1-2 parts of Herba portulacae, 3-4 parts of dark plum, sealwort 2-3
Part, 1-2 parts of dandelion, 1-2 parts of honeysuckle, 1-3 parts of fructus amomi, 1-2 parts of almond, 2-3 parts of cassia seed, 1-2 parts of the coptis, Poria cocos 1-3
Part, 2-3 parts of Rhizoma Atractylodis Macrocephalae, 1-3 parts of Radix Salviae Miltiorrhizae, 1-2 parts of dried orange peel, 2-4 parts of hawthorn, 1-2 parts of hoveniae semoveniae semen.
Pueraria lobata has pueraria isoflavone and Puerarin：Brain and the effect of coronal vascular flow amount can be increased, also with low vascular resistence
With Green Tea Extract and improve SOD active functions.Inhibit ethyl alcohol stomach absorb and with accelerating alcohol katabolism, to reduce body blood
Determining alcohol, to play relieving alcoholism and preventing drunkenness effect；There is hoveniae semoveniae semen Central nervous depressant, anti peroxidation of lipid and blood pressure lowering to make
With；Flower of kudzuvine can be effectively improved Ethanol concentration in rat blood, reduce by three fat of liver oil in blood, improve the oxidation resistance of hepatic tissue, promote fat
Fat acid oxidase decomposes, and reduces lipid level；Chinese yam, fructus amomi, almond hydrolysis generation glucose can inhibit alcohol dehydrogenase, slow down second
The generation of aldehyde；Herba portulacae contains Herba portulacae polysaccharide, contains the ingredients such as gallic acid, citric acid, malic acid, the above ingredient in dark plum
With antiviral, oxidation resistant effect, the oxidation resistance of body can be improved, accelerates acetal dehyde decomposition；Studies have shown that sealwort carries
Take object and dandelion extract that there is obvious protective function to acute hepatic injury mice；Main chemical compositions have flavones in honeysuckle
Class, organic acid, glycoside and volatile oil etc..Mainly there is with the effect of honeysuckle hepatic cholagogic triterpenoid saponins wherein in glycoside
It closes；Cassia seed extracting solution has an impact rat liver CYP450 enzymes enzymatic activity, mRNA and protein expression level, has and reduces liver
The effect of lipid precipitation；The coptis damages the protective effect having to liver mitochondrion caused by insulin resistance rat oxygen radical；
Poria cocos, Rhizoma Atractylodis Macrocephalae, hawthorn have detoxication to hepatic injury；Radix Salviae Miltiorrhizae can promote microcirculation, inhibit liver cell damage and death,
Improve intracellular anaerobic environment, promote liver cell synthesis and secretion, there is obvious protective function to liver.
A kind of preparation method of relieving alcoholism and protecting liver ferment, two step enzymatic isolation methods extraction integration of drinking and medicinal herbs raw material obtain enzymolysis liquid, will digest
Liquid and water mixing, enzymolysis liquid and water are mixed by the component of following parts by weight：30-45 parts of enzymolysis liquid, 55-70 parts of water；Auxiliary material is added,
Beneficial flora is accessed after sterilization treatment and carries out multi-stage combination fermentation, subsequently enters ageing stage, 3000- after the completion of conversion
4000rpm centrifuges 5-10min, collects supernatant；
The present invention is compared with traditional high temperature processing procedure, by biology extraction and microbial conversion process, not only by medicine
It eats the active skull cap components in homologous raw material completely to retain and discharge, and converts conducive to absorption of human body part macromolecular components to
With the small molecule ingredient of metabolism, while active secondary metabolites are generated, improves the type of active constituent in relieving alcoholism and protecting liver ferment
And function.
After Enzymatic Extraction and probiotics fermention, puerarin content processes Puerarin in aqueous solution relative to conventional high-temperature and carries
It is nearly 7 times high.Puerarin has reduction vascular resistence, inhibits the absorption of ethyl alcohol stomach and accelerating alcohol katabolism, to reduce body
Blood alcohol concentration reduces endotoxin (LPS) concentration and Cytochrome P450 2E1 (CYP2E1) is inhibited to express, and reduces free radical and generates,
To mitigate hepatic injury caused by oxidative stress, reach relieving alcoholism and protecting liver effect.
It is detected, sends out to the ferment product and through extracting the amino acid content in solution made from high temperature concocting method
It is existing, by the amino acid content of relieving alcoholism and protecting liver ferment made from this method nearly 10 are improved compared with amino acid content in conventional method
Times.Body metabolic disorder caused by excessively intake alcohol is adjusted in the increase of amino acid content, and the oxidation in stable cytosol is also
Former current potential accelerates tricarboxylic acid cycle, promotes the decomposition of acetaldehyde, reduce the damage of hepatic tissue.
The free radical scavenging activity curve that aqueous solution is processed to the ferment product and conventional high-temperature is detected, relieving alcoholism and protecting liver ferment
The free radical scavenging activity of element improves 92% compared with than tradition processing aqueous solution, has higher oxidation resistance.Alcohol is thin to liver
The toxicity of born of the same parents can make the lipid components excessive oxidation on liver plasma membrane surface, destroy liver cell by the metabolism of influence liver cell
Film.And internal superoxide dismutase, malonaldehyde, glutathione peroxidase, glutathione occur bright after excessive drinking
Aobvious to change, body, which generates a large amount of oxygen radical, makes oxidation reaction enhance, and generates a large amount of free radicals, very to the murder by poisoning of body tissue
Greatly.Therefore, the oxidation resistance of research relieving alcoholism and protecting liver ferment has important directive significance to liver protecting.
The auxiliary material of addition press enzymolysis liquid and water mixed system parts by weight percentages, for it is following any one or arbitrary two
Kind and two or more mixing：
Edible glucose 3-8%
Edible sucrose 2-5%
Food yeast powder 1-2%.
In order to increase the mouthfeel of relieving alcoholism and protecting liver ferment, following component is added, additive amount is by tasting binding function sugar work(
What the addition range of effect finally determined.In supernatant be added with supernatant by weight percentage it is following any one or appoint
Meaning two kinds and two or more mixtures：
Honey 3-6%
Antierythrite 0.5-1%
Oligofructose 0.5-1%
The beneficial flora is prebiotic bacillus amyloliquefaciens, bacillus licheniformis, bacillus amyloliquefaciens, natto gemma
Bacillus, bacillus cereus, Rhizopus oryzae, acetic acid bacteria, mucor, cloth Laplace yeast S. cerevisiae bacterium, increases bacillus coagulans
Fragrant saccharomycete, Wei Shi yeast, Hansenula anomala, schizosaccharomyces pombe, glutinous rhodotorula, pichia farinose, production glycerine vacation silk
Yeast, candida utili, lactobacillus plantarum, Lactobacillus casei, streptococcus thermophilus, lactobacillus gasseri, Bifidobacterium, calf Portugal
Grape coccus, lactobacillus acidophilus, curls arbitrary two kinds and two or more mixing in lactobacillus at staphylococcus xylosus.Selection
Above-mentioned strain is common probiotics fermention flora.
The beneficial flora access amount is accessed according to the 2-10% of enzymolysis liquid volume.The beneficial flora access amount is according to enzyme
Solve the 2-5% accesses of liquid product.2%-10% is allowed inoculation range；2%-5% is conventional optimal inoculation range.
Multi-stage combination fermentation be arbitrary two kinds and two kinds in aerobic fermentation, amphimicrobian fermentation, anaerobic fermentation with
On mixed fermentation mode.Ferment fermentation is mostly a kind of bacterium single fermentation, preferably at most yeast and lactic acid in presently disclosed patent
Mixed fermentation, this patent uses the multi-stage combination zymotechnique, the advantages of multi-stage combination zymotechnique to be：Multi-stage combination fermentation energy
It is enough further to discharge and convert the active constituent in integration of drinking and medicinal herbs raw material, while generating different types of abundant prebiotic stimulation metabolism
Product enhances the type and content of active constituent in ferment.
Multi-stage combination fermentation is 30-37 DEG C, 150-200rpm aerobic fermentations 1-2 days, 25-37 DEG C of 50-100rpm are simultaneous
Property arbitrary two kinds and two or more mixed fermentation technologies in 1-14 days of anaerobic fermentation 1-5 days, 20-37 DEG C of standing for fermentation.
The common time of ferment after-ripening at present is：The time in ageing stage is 1-6 months, and temperature is 10-25 DEG C.
Two step enzymatic isolation methods extraction integration of drinking and medicinal herbs raw material specific steps include：
S101：Water is added after integration of drinking and medicinal herbs raw material is crushed, mixed liquor carries out broken wall, the agitated carry out first step of shell-broken liquid
Enzymolysis and extraction；
Broken wall condition is including but not limited to 30-40 DEG C of ultrasound 60-90min, sporoderm-broken rate 85-95%.
The extracting method of first step enzymolysis and extraction：40-60 DEG C, 150-200rpm stirrings, pH 3.5-5.5, extraction time
60-90min；In S102, the extracting method of second step enzymolysis and extraction：50-70 DEG C, 100-200rpm stirrings, pH 7.0-8.5,
Extraction time 30-60min.
Enzyme used in first step enzymolysis and extraction is following arbitrary two kinds and two or more in terms of weight permillage with mixed liquor
Mixing：
S102：By the mixed liquor after first step enzymolysis and extraction, stirring carries out second step enzymolysis and extraction.
Enzyme used in second step enzymolysis and extraction with mixed liquor in terms of weight permillage, for it is following any one or it is two kinds arbitrary
And two or more mixing：
For integration of drinking and medicinal herbs raw material mostly using traditional high temperature concocting method processing extraction, the present invention uses two step enzyme methods at present,
There are no be reported in the preparation process of ferment for two step enzyme methods；It can pass through the association of variety classes enzyme using two step enzymatic isolation methods
Same-action fully digests and releases the active ingredient in integration of drinking and medicinal herbs raw material, the active ingredient in integration of drinking and medicinal herbs raw material is made fully to release
It is put into enzymolysis liquid, puerarin content processes Puerarin in aqueous solution relative to conventional high-temperature and improves nearly 7 times.Amino acid content
Nearly 10 times are improved compared with amino acid content in conventional method.Enzyme used in first step enzymolysis and extraction is acidicenzym, second step enzymolysis
Extraction enzyme used is neutral or alkaline enzyme, by the synergistic effect of variety classes enzyme, fully digests and release integration of drinking and medicinal herbs original
Active ingredient in material.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention is the test material of this field routine, can pass through commercial channel
It is commercially available.
Embodiment 1
In parts by weight, 40 DEG C of 2 parts of low temperature drying integration of drinking and medicinal herbs flower of kudzuvine, 4 parts of pueraria lobata, 3 parts of Chinese yam, 2 parts of Herba portulacae, crow are taken
4 parts of plum, 2 parts of sealwort, 2 parts of dandelion, 2 parts of honeysuckle, 3 parts of fructus amomi, 2 parts of almond, 2 parts of cassia seed, 1 part of the coptis, 3 parts of Poria cocos,
2 parts of Rhizoma Atractylodis Macrocephalae, 3 parts of Radix Salviae Miltiorrhizae, 2 parts of dried orange peel, 3 parts of hawthorn are crushed to and are mixed well less than after 36 mesh for totally 42 parts.Take above-mentioned mixing dry
65 parts of water, mixing is added in powder.
30 DEG C of ultrasound 60min broken wall treatments of integration of drinking and medicinal herbs raw material mixed liquor, sporoderm-broken rate 85%.Mixed liquor after broken wall
150rpm stirring enzymolysis 60min, temperature 50 C, pH 4.0.Extraction enzyme used and mixed liquor are as follows in terms of weight permillage：
The integration of drinking and medicinal herbs mixed liquor after first step Enzymatic Extraction is taken to carry out second step biology extraction.In parts by weight, first
Integration of drinking and medicinal herbs mixed liquor after step enzyme method extraction stirs enzymolysis and extraction 30min, temperature 70 C, pH 7.0 through 100rpm.Extraction institute
It is as follows in terms of weight permillage with enzyme and mixed liquor：
It is as follows by the parts by weight percentages of enzymolysis liquid and water mixed system that integration of drinking and medicinal herbs digests mixed liquor addition auxiliary material：
Edible glucose 3%
Food yeast powder 2%
80 DEG C of sterilizing 15min of integration of drinking and medicinal herbs enzymolysis liquid.Bacterium amount is connect according to the 2% of enzymolysis liquid volume access activated receive afterwards
The aerobic fermentation 2% activated plant of access again after 1-2 days under 35 DEG C, 150-200rpm of beans bacillus, bacillus licheniformis
Object lactobacillus, Lactobacillus casei, streptococcus thermophilus are left to ferment 1 week at 37 DEG C.
Relieving alcoholism and protecting liver ferment after fermentation, enters 3 months ageing stages at a temperature of 37 DEG C at 20 DEG C.
The relieving alcoholism and protecting liver ferment 3000rpm centrifugation 5min that after-ripening is completed are taken, its supernatant is collected.
Honey and functional sugar are added in supernatant, it is as follows according to being mixed with supernatant weight percent meter respectively：
Honey 3%
Erythrose alcohol 1%
Oligofructose 1%
Functional sugar and honey are added in relieving alcoholism and protecting liver ferment, and 200rpm stirrings 30min is mixed well, as relieving alcoholism and protecting liver
Ferment product.To the active constituent Puerarin detection in the ferment product as shown in Figure 1, conventional high-temperature processes pueraria lobata in aqueous solution
Cellulose content is 69.33mg/L, and puerarin content is 534.8mg/L in relieving alcoholism and protecting liver ferment.It can be seen that through Enzymatic Extraction and benefit
After raw bacterium fermentation, puerarin content improves nearly 7 times.Puerarin inhibits ethyl alcohol stomach to absorb and and add with vascular resistence is reduced
Fast ethanol degradation metabolism reduces endotoxin (LPS) concentration and inhibits Cytochrome P450 2E1 to reduce body blood alcohol concentration
(CYP2E1) it expresses, reduces free radical and generate, to mitigate hepatic injury caused by oxidative stress, reach relieving alcoholism and protecting liver effect.
Embodiment 2
In parts by weight, 35 DEG C of 1 part of low temperature drying integration of drinking and medicinal herbs flower of kudzuvine, 2 parts of Chinese yam, 1 part of Herba portulacae, 4 parts of dark plum, Pu are taken
2 parts of public English, 1 part of honeysuckle, 2 parts of fructus amomi, 1 part of almond, 3 parts of cassia seed, 1 part of the coptis, 3 parts of Poria cocos, 3 parts of Rhizoma Atractylodis Macrocephalae, 3 parts of Radix Salviae Miltiorrhizae,
1 part of dried orange peel, 2 parts of hawthorn are crushed to and are mixed well less than after 36 mesh for totally 30 parts.Above-mentioned mixed powder is taken, 45 parts of water is added, is mixed
It is even.
30 DEG C of ultrasound 70min broken wall treatments of integration of drinking and medicinal herbs raw material mixed liquor, sporoderm-broken rate 87%.Mixed liquor after broken wall
150rpm stirring enzymolysis 70min, temperature 50 C, pH 4.5.Extraction enzyme used and mixed liquor are as follows in terms of weight permillage：
The integration of drinking and medicinal herbs mixed liquor after first step Enzymatic Extraction is taken to carry out second step biology extraction.By weight percentage,
Integration of drinking and medicinal herbs mixed liquor after first step Enzymatic Extraction stirs enzymolysis and extraction 50min, temperature 70 C, pH 7.5 through 150rpm.It carries
Take enzyme and mixed liquor used as follows in terms of weight permillage：
Integration of drinking and medicinal herbs digests mixed liquor addition auxiliary material C, N sources by the parts by weight percentages of enzymolysis liquid and water mixed system such as
Under：
Edible sucrose 4%
PURE WHEY 1%
80 DEG C of sterilizing 20min of integration of drinking and medicinal herbs enzymolysis liquid.Afterwards according to the 3% activated cloth Laplace ferment of access of enzymolysis liquid volume
Female bacterium, S. cervisiae, the utilizing flavoring yeast amphimicrobian under 25-37 DEG C, 50-100rpm rotating speeds ferment 1-5 days.One time fermentation
After the completion, bacterium amount access lactobacillus gasseri, Bifidobacterium, lactobacillus acidophilus is connect according still further to 5% to be left to ferment 15 days in 35 DEG C.
Relieving alcoholism and protecting liver ferment after fermentation, enters 3 months ageing stages in 2 grades of joints at 15 DEG C.
The relieving alcoholism and protecting liver ferment 3000rpm centrifugation 8min that after-ripening is completed are taken, its supernatant is collected.
Functional sugar is added in supernatant, it is as follows according to being mixed with supernatant weight percent meter respectively：
Erythrose alcohol 1%
Oligofructose 1%
Functional sugar is added in relieving alcoholism and protecting liver ferment, and 200rpm is stirred and evenly mixed and is cooled further to 22 DEG C.Honey is added
Into uniformly mixed relieving alcoholism and protecting liver ferment, 200rpm stirrings 20min is mixed well, as ferment product.To the ferment product
It is detected with through extracting the amino acid content in solution made from high temperature concocting method, it is found that amino acid contains in conventional method
Amount is 0.343 × 103Mg/L, and the amino acid content for passing through relieving alcoholism and protecting liver ferment made from this method is 3.96 × 103Mg/L,
Amino acid content improves nearly 10 times.It is disorderly that body metabolism caused by excessively intake alcohol is adjusted in the increase of amino acid content
Disorderly, stablize the oxidation-reduction potential in cytosol, accelerate tricarboxylic acid cycle, promote the decomposition of acetaldehyde, reduce the damage of hepatic tissue.
Embodiment 3：
In parts by weight, 35 DEG C of 5 parts of low temperature drying integration of drinking and medicinal herbs pueraria lobatas, 3 parts of Chinese yam, 2 parts of Herba portulacae, 3 parts of dark plum, Pu are taken
1 part of public English, 2 parts of honeysuckle, 3 parts of fructus amomi, 2 parts of almond, 2 parts of cassia seed, 3 parts of Poria cocos, 2 parts of Rhizoma Atractylodis Macrocephalae, 3 parts of Radix Salviae Miltiorrhizae, 1 part of dried orange peel,
3 parts of hawthorn is crushed to and is mixed well less than after 36 mesh for totally 35 parts.Above-mentioned mixed powder is taken, 55 parts of water, mixing is added.
35 DEG C of ultrasound 70min broken wall treatments of integration of drinking and medicinal herbs raw material mixed liquor, sporoderm-broken rate 89%.Mixed liquor after broken wall
150rpm stirring enzymolysis 70min, temperature 60 C, pH 5.0.Extraction enzyme used and mixed liquor are as follows in terms of weight permillage：
The integration of drinking and medicinal herbs mixed liquor after first step Enzymatic Extraction is taken to carry out second step biology extraction.In parts by weight, first
Integration of drinking and medicinal herbs mixed liquor after step enzyme method extraction stirs enzymolysis and extraction 50min, temperature 60 C, pH 7.5 through 150rpm.Extraction institute
It is as follows in terms of weight permillage with enzyme and mixed liquor：
It is as follows by the parts by weight percentages of enzymolysis liquid and water mixed system that integration of drinking and medicinal herbs digests mixed liquor addition auxiliary material：
Edible glucose 6%
Food yeast powder 1%
80 DEG C of sterilizing 20min of integration of drinking and medicinal herbs enzymolysis liquid.Bacterium amount is connect according to 2% access activated solution starch gemma bar afterwards
Bacterium, bacillus licheniformis aerobic fermentation 1-2 days under 35 DEG C, 150-200rpm connect the inferior ferment of the abnormal Chinese of bacterium amount access according still further to 2%
Mother, the facultative anaerobic fermentation of schizosaccharomyces pombe 5 days finally connect bacterium amount access lactobacillus plantarum, cheese breast bar according to 5%
Bacterium, 25 DEG C of streptococcus thermophilus are left to ferment 14 days.
Relieving alcoholism and protecting liver ferment ferments 2 weeks at a temperature of 37 DEG C.Then enter 4 months ageing stages at 20 DEG C.
The relieving alcoholism and protecting liver ferment 4000rpm centrifugation 5min that after-ripening is completed are taken, its supernatant is collected.
Honey and functional sugar are added in supernatant, it is as follows according to being mixed with supernatant weight percent meter respectively：
Honey 4%
Erythrose alcohol 1%
Functional sugar and honey are added into uniformly mixed relieving alcoholism and protecting liver ferment, and 150rpm stirrings 30min is mixed well, i.e.,
For relieving alcoholism and protecting liver ferment product.The free radical scavenging activity curve that aqueous solution is processed to the ferment product and conventional high-temperature is examined
It surveys, as a result as shown in the figure：Fig. 2 relieving alcoholism and protecting liver ferment IC50 values are that the IC50 values that 0.088, Fig. 3 conventional high-temperatures process aqueous solution are
0.1693, the free radical scavenging activity of relieving alcoholism and protecting liver ferment improves 92% compared with than tradition processing aqueous solution, has higher antioxygen
Change ability.Alcohol can make the lipid components mistake on liver plasma membrane surface to the toxicity of liver cell by the metabolism of influence liver cell
Degree oxidation, destroys liver plasma membrane.And internal superoxide dismutase, malonaldehyde, glutathione peroxidase after excessive drinking
Enzyme, glutathione are substantially change, and body, which generates a large amount of oxygen radical, makes oxidation reaction enhance, and generates a large amount of free radicals,
It is very big to the murder by poisoning of body tissue.Therefore, the oxidation resistance of research relieving alcoholism and protecting liver ferment has important finger to liver protecting
Lead meaning.
Experimental result is to sum up stated, puerarin content, amino acid content etc. in the relieving alcoholism and protecting liver ferment prepared by the present invention
Active ingredient content and oxidation resistance are above in conventional high-temperature processing procedure aqueous solution related content and index, therefore use
Relieving alcoholism and protecting liver ferment prepared by the method for the present invention is accelerating tricarboxylic acid cycle and is promoting the decomposition of acetaldehyde, improves ethanol in blood
And triglyceride concentration, improving oxidation resistance of hepatic tissue etc. has important potential, for alleviating people's excessive drinking
Caused by the exploitations of hepatic injury and other ferment products there is important directive significance.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or
It is realized using the prior art, details are not described herein；Above example and attached drawing are only used to illustrate the technical scheme of the present invention not
It is limitation of the present invention, is described the invention in detail with reference to preferred embodiment, the ordinary skill people of this field
Member is it should be appreciated that variation, remodeling, the addition that those skilled in the art are made in the essential scope of the present invention
Or replace the claims that should also belong to the present invention without departure from spirit of the invention.
Claims (10)
1. a kind of relieving alcoholism and protecting liver ferment, it is characterised in that：It is prepared with integration of drinking and medicinal herbs raw material, integration of drinking and medicinal herbs raw material is by following heavy
The group of amount part is grouped as：1-2 parts of flower of kudzuvine, 3-5 parts of pueraria lobata, 1-3 parts of Chinese yam, 1-2 parts of Herba portulacae, 3-4 parts of dark plum, 2-3 parts of sealwort,
1-2 parts of dandelion, 1-2 parts of honeysuckle, 1-3 parts of fructus amomi, 1-2 parts of almond, 2-3 parts of cassia seed, 1-2 parts of the coptis, 1-3 parts of Poria cocos,
2-3 parts of Rhizoma Atractylodis Macrocephalae, 1-3 parts of Radix Salviae Miltiorrhizae, 1-2 parts of dried orange peel, 2-4 parts of hawthorn, 1-2 parts of hoveniae semoveniae semen.
2. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 1, it is characterised in that：It is including step：Two
Step enzymatic isolation method extraction integration of drinking and medicinal herbs raw material obtains enzymolysis liquid, and enzymolysis liquid and water are mixed, auxiliary material is added, beneficial flora is accessed after sterilizing
It carries out multi-stage combination fermentation and supernatant is finally collected by centrifugation subsequently into the ageing stage.
3. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 2, it is characterised in that：The auxiliary material of addition presses enzyme
The parts by weight percentages for solving liquid and water mixed system, for following any one or arbitrary two kinds and two or more mixing：
Edible glucose 3-8%
Edible sucrose 2-5%
Food yeast powder 1-2%.
4. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 2, it is characterised in that：It is added in supernatant
With following any one or arbitrary two kinds and the two or more mixtures of supernatant by weight percentage：
Honey 3-6%
Antierythrite 0.5-1%
Oligofructose 0.5-1%.
5. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 2, it is characterised in that：The beneficial flora connects
Enter amount to access according to the 2-10% of enzymolysis liquid volume；Preferably, the beneficial flora access amount according to enzymolysis liquid volume 2-5%
Access.
6. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 2, it is characterised in that：The multi-stage combination hair
Ferment includes arbitrary two kinds and two or more mixed fermentation modes in aerobic fermentation, amphimicrobian fermentation, anaerobic fermentation.
7. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 2, it is characterised in that：Two step enzymatic isolation methods extract
The specific steps of integration of drinking and medicinal herbs raw material include：
S101：After integration of drinking and medicinal herbs raw material is crushed plus water, mixed liquor carry out broken wall, and the agitated progress first step enzymolysis of shell-broken liquid carries
It takes；
S102：By the mixed liquor after first step enzymolysis and extraction, stirring carries out second step enzymolysis and extraction.
8. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 7, it is characterised in that：In S101, the first step
Enzyme used in enzymolysis and extraction is following arbitrary two kinds and two or more mixing in terms of weight permillage with mixed liquor：
9. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 7, it is characterised in that：In S102, second step
Enzyme used in enzymolysis and extraction with mixed liquor in terms of weight permillage, for it is following any one or it is two kinds and two or more mixed arbitrary
It closes：
10. a kind of preparation method of relieving alcoholism and protecting liver ferment according to claim 7, it is characterised in that：In S101, the first step
The extracting method of enzymolysis and extraction：40-60 DEG C, 150-200rpm stirrings, pH 3.5-5.5, extraction time 60-90min；In S102,
The extracting method of second step enzymolysis and extraction：50-70 DEG C, 100-200rpm stirrings, pH 7.0-8.5, extraction time 30-60min.
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| CN115812948A (en) * | 2022-07-05 | 2023-03-21 | 育米生物科技(云南)有限公司 | Chinese medicinal material alcohol effect dispelling enzyme and preparation method thereof |
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| CN111213875A (en) * | 2018-12-24 | 2020-06-02 | 国润民富科技发展有限公司 | Preparation method of cerasus humilis composite Chinese herbal medicine enzyme cream with functions of dispelling effects of alcohol and protecting liver |
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| CN112155144A (en) * | 2020-09-29 | 2021-01-01 | 江苏茅宝葛业有限公司 | Radix puerariae enzyme solid beverage and preparation method thereof |
| CN112931866A (en) * | 2021-03-29 | 2021-06-11 | 山东百德生物科技有限公司 | Edible amino acid enzyme and preparation method thereof |
| CN115812948A (en) * | 2022-07-05 | 2023-03-21 | 育米生物科技(云南)有限公司 | Chinese medicinal material alcohol effect dispelling enzyme and preparation method thereof |
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Application publication date: 20180814 |