CN107927500A - A kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali - Google Patents
A kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali Download PDFInfo
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- CN107927500A CN107927500A CN201711223703.0A CN201711223703A CN107927500A CN 107927500 A CN107927500 A CN 107927500A CN 201711223703 A CN201711223703 A CN 201711223703A CN 107927500 A CN107927500 A CN 107927500A
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 208000018556 stomach disease Diseases 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
- A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Water Supply & Treatment (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
A kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, it is related to a kind of beverage, it is combined by ultrasonic wave with dynamic countercurrent circulation, so that the fluffy middle polyphenol recovery rate of alkali greatly improves, play a multiplier effect, ultrasonic wave aids in, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in water miscible functional components, the fluffy middle polyphenols recovery rate of alkali improves 30% 70% compared to traditional extraction process recovery rate, both rich in antioxidant contents such as the fluffy middle Polyphenols of alkali, there are the essential trace elements of the human body and abundant amino acid again, it can meet pursuit of the people to nutrition and health care double effects, the fast pace life of present people is adapted at the same time.
Description
【Technical field】
The invention belongs to beverage production field, more particularly to a kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali.
【Background technology】
Fluffy alkali is annual herb plant, is generally born on the saline-alkali wastelands such as beach, lakeside, river valley, is up in salt content
3% intertidal zone can also be grown, and be a kind of typical salt-soda soil indicator plant.Alkali is fluffy to be had soil ecological protection and improves work
With being known as " pionner " of alkaline land improvement.Research finds that the fluffy fresh and tender cauline leaf of alkali is full of nutrition, rich in fat, albumen
Matter, crude fibre, mineral matter, trace element and multivitamin etc., great Development volue.Suaeda salsa is annual herb salification
Plant, the fluffy middle nutritional ingredient of alkali is complete and enriches, and the fluffy stem leaf protein matter of alkali, fat, carbohydrate three nutritious elements content are equal
Higher, its seed fat content accounts for the 36.5% of dry matter, and higher than soybean, the protein content of fresh and tender cauline leaf accounts for dry matter
40%, 16 kinds of amino acid are measured altogether in Suaeda salsa, wherein have 8 kinds of essential amino acids, and content is higher, has expansion blood
The effect of pipe, enhancing body immunity, decompression, hypoglycemic, prevention and cure of cardiovascular disease；Also containing a variety of needed by human micro- during alkali is fluffy
Secondary element and abundant vitamin, wherein calcium, magnesium, zinc, iron, nickel content are enriched, its calcium, phosphorus, iron and core yellow cellulose content are far above
The vegetables such as spinach, tomato and carrot, Vitamin C content are higher than or equivalent to general vegetables, and vitamin B content is general vegetables
5-8 times, far above spirulina；Invention Announce number is that the patent document of CN106578918A discloses a kind of fluffy nutrition of selenium-rich alkali
Healthy fine dried noodles and its production technology, patent document research demonstrate fluffy middle high 10 times or so of the more general food of Se contents of alkali, and
Selenium is referred to as " king of anti-cancer " in body trace element by scientist, has anticancer, pre- preventing tumor, the generation of hepatopathy, raising
The effect of body immunity；Invention Announce number is：The patent document of CN103027962A discloses a kind of Suaeda salsa flavones
Extraction and content assaying method, it is higher that patent document research demonstrates Suaeda salsa (S.salsa) leaf flavonoids content, and has
Stronger antioxidation activity, plant flavone have the multiple efficacies such as anti-oxidant, antitumor, antiatherosclerosis, anti-inflammatory.
The one kind of pigment as polyphenol, the pigment during alkali is fluffy are the valuable sources of natural pigment, and the pigment is through researching and analysing
For water-soluble anthocyanidin.Anthocyanidin has higher security performance, and have anti-mutation, resist as natural pigment
The healthcare functions such as oxidation, blood pressure lowering, protection liver, belong to functional food additives.Polyphenols is referred to as " the 7th class battalion
Support element ".Polyphenol is natural " antioxidant ", possesses superpower oxidation resistance, can prevent body from aoxidizing, and can remove human body
The free radical of generation, delaying skin aging；Suppress the decomposition of collagen, play crease-resistant function.Moreover, it or it is good
" anticarcinogen ", suppresses the activation of carcinogen, resists cell cancerization.
It is external that polyphenol can promote lipid and bile acid to exclude, and reduces containing for cholesterol and triglycerides in blood plasma and liver
Amount, has prevention of arterial hardening, reduces blood pressure and blood fat, also the prevention effect such as thrombus, very strong antioxidation, have and prolong
The functions such as slow aging, pre- anti-cancer.
Antioxidation activity analysis shows alkali is fluffy to O-2Free radical has stronger scavenging action, often edible to be conducive to remove body
Interior excessive oxygen radical, so as to be good for one's health and long-lived.Alkali is fluffy have at the same time as a kind of ancient edible vegetables it is higher
Medical value, according to《A Supplement to the Compendium of Materia Medica》Introduction, salty cool, the nontoxic, heat-clearing of its property, disperse accumulation.Modern medicine study is also sent out
Existing, Suaeda plant has the effects such as hypoglycemic, decompression, expansion blood vessel, prevention heart disease and enhancing body immunity, suitable for the heart
The prevention of vascular system disease, has notable health-care effect especially for the elderly, hypertensive patient.But alkali is fluffy at present
Processing and eating method are all in the primary stage, before alkali fluffy superior biological nature and effect determine its wide application
Scape, its research and development have very big economic value and social value.
CN 201510166655.0 discloses a kind of method that water extract method makes the fluffy beverage of health food alkali, and collection is new
Fresh Suaeda salsa aubergine blade, dries in the shade after cleaning repeatedly, crushes, and crosses 30 mesh sieves；According to feed liquid mass ratio 1: 25~30, water
Extraction 2 times, merges leaching liquor twice, filters；According to leaching liquor: the mass ratio of water is 1: 1, mass percentage 12%~
15% white granulated sugar, the citric acid of mass percentage 0.04%~0.06%, the honey of mass percentage 3%~5%, matter
The Vc of amount percentage 0.05%~0.08% is uniformly mixed, to obtain the final product.Temperature 50 C~60 DEG C of the flooding, time
50~60min, carries out pasteurize after mixing, and the method is high pol there are problem, and Consumer groups are limited, unfavorable strong
Health.Secondly, water-soluble polyphenols class material recovery rate is low, and pasteurize can destroy anthocyan material stability, it is impossible to retains
Its functional characteristic.
CN 201510398358.9 discloses a kind of processing method of mineral water, comprises the following steps：1) by natural ore deposit
Material is put into container after being crushed to 0.1~0.5 centimetre of little particle washing；2) give in container by weight the pure of injection 2 to 1
Water purification；3) again to be passed through in container 0.3~10 it is small when ozone, put only five minutes；4) will finally be put only from the water outlet of container
Water outflow afterwards.Natural mineral matter described in the method refers to the jade of the composition containing mineral matter, with single nutrient component, only rich
Containing mineral matter.
CN 201510467632.3 discloses a kind of Suaeda salsa beverage, and by weight percentage, the beverage includes following
Component：Suaeda salsa 8%~10%, glucose 10%~15%, purified water 70%~75%, sodium acid carbonate 2%~5% and right
Nipagin A 1%~3%.The invention obtains stoste and solid slag, solid slag is added water immersion adds using segment pressure juice
Heat, obtains the fluffy juice of alkali, then by boiling, cools down, and the technique such as sterilizing, the fluffy juice of alkali that the method obtains passes through the links such as high temperature sterilization,
The stability of vitamin substances is subject to heavy damage, and product with the addition of substantial amounts of glucide, be unfavorable for health.
In conclusion alkali is fluffy to have all multi-nutrients, there is good health-care efficacy, have great economic value with
Social value, and also contain day without one kind also without a kind of method of the energy fluffy middle polyphenol substance of high efficiency extraction alkali on Vehicles Collected from Market
The beverage of right plant polyphenol, mineral matter and amino acid.
【The content of the invention】
In order to overcome the shortcomings of in background technology, the present invention works out a kind of ultrasound wave auxiliary enzyme method and prepares the fluffy beverage of alkali
Method, is combined so that the fluffy middle polyphenol recovery rate of alkali greatly improves, and plays and gets twice the result with half the effort by ultrasonic wave with dynamic countercurrent circulation
Effect, in addition ultrasonic wave auxiliary, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in it is water miscible
Functional components, and then it is made a kind of rich in the fluffy water-soluble polyphenols of alkali, the fluffy beverage of the alkali of trace element and abundant amino acid.
For achieving the above object, the present invention adopts the following technical scheme that：
A kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, includes the following steps：
Step 1, fresh Leaves of Suaeda Salsa is gathered, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, the material after crushing is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1:
Add water at 20~1: 50, adds 2%~4% (w/w) cellulase, adds 0.05%~0.08% vitamin C, enzymolysis time
20min~30min, 30 DEG C~50 DEG C, ultrasonic power 50w~200w of hydrolysis temperature, extraction time are 1~2 time, merge extraction
Liquid；
Step 3, leaching liquor carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the water in step 1 used in wet pulverizing, which calculates, to exist
In step 2 in 1: 20~1: 50 feed liquid mass ratio.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, water used is deionization in step 1 and step 2
Water.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the wet pulverizing technique in step 1 can be replaced
Dry pulverization process, is specially：Fresh Leaves of Suaeda Salsa is gathered, food purifying machine is put into and cleans 1 time~3 times, utilize vegetable drying
Sterilization machine is dried, and temperature is 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is spare to cross 20~40 mesh sieves.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the leaching liquor its mineral described in step 2
Matter component and content are as follows：Ca2+μ g/L of 2985 μ g/L~5200, Mg2+μ g/L of 1743 μ g/L~2791, Fe2+162 μ g/L~185
μg/L、Mn2+μ g/L of 5.11 μ g/L~8.03, Zn+μ g/L of 5.27 μ g/L~8.51, K+μ g/L of 0.8 μ g/L~3.0, Na+1746μg/
The μ g/L of μ g/L of L~6899,200 μ g/L of total dissolved solid~400.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the reverse osmosis unit system configuration described in step 3
For：Pretreatment system, smart filtration system, corollary system；Pretreatment system is filtered by mechanical filter, adsorption filter, security personnel
Device forms；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system is made of water purification pot, electrical control system.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, ultrafiltration described in step 3 are hollow-fibre membrane ultrafiltration
To remove the microorganisms such as bacterium in extracting solution, mould, a diameter of 0.01 μm~0.001 μm of the hollow-fibre membrane, feed rate
For 900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration is the μ g/L of 200 μ g/L~400, the ultrafiltration time
100min~400min, backwash cycle 10min~40min, backwash time 20min~120min, upper operation 10min~40min,
Upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.
Due to using technical solution as described above, the present invention has the advantages that：
1st, the present invention is combined by ultrasonic wave with dynamic countercurrent circulation so that and the fluffy middle polyphenol recovery rate of alkali greatly improves,
Play a multiplier effect；
2nd, the present invention aided in by ultrasonic wave, aqueous enzymatic extraction and ultrafiltration technology triple combination greatly remain alkali it is fluffy in
Water miscible functional components, the fluffy middle polyphenols recovery rate of alkali improve 30%- compared to traditional extraction process recovery rate
70%, aqueous enzymatic method combination ultrasonic wave physical process, the very big acceleration produced using it, strong cavitation effect, high vibration
Etc. property, asked when reducing extraction, avoid temperature from changing the activity change to active ingredient；The ultrasonic wave dynamic countercurrent of the present invention is auxiliary
Help enzyme and ultrafiltration technology is combined the method simple production process of the extraction fluffy middle polyphenols of alkali, to expend the time few, reduce
Production work intensity, has product active ingredient high, reduces the advantages that temperature changes the adverse effect brought.
3rd, the present invention effectively eliminated using coarse filtration and ultrafiltration the impurity such as body refuse in extracting solution, suspended matter, algae and
The microorganism such as bacterium, mould in extracting solution, the various nutritional ingredients and trace element that remain the fluffy extracting solution of alkali of high degree,
It is easy to operate, it is suitable for industrialized production, compared to traditional filter method, hyperfiltration technique is a kind of purification for being widely used in water,
Pollution-free, energy consumption is low, and separator is simple, flow is short, easy to operate, easily controllable and maintenance, installation area are small.Normal
Temperature is lower to be carried out, and mild condition is without component damage, thus the separation and enrichment of the particularly suitable material to this product (thermo-responsive), right
The recycling of micro constitutent in this product is highly effective.Mechanical filter filtering can go to extract in ultrafiltration technology pretreatment system
Body refuse and fiber in liquid etc., after through adsorption filter filtering can remove the impurity such as oil removal and algae, and then pass through
The hollow-fibre membrane ultrafiltration of ultrafiltration technology essence filtration system is to remove the microorganisms such as bacterium in extracting solution, mould.
4th, the fluffy beverage of alkali produced by the present invention is rich in the fluffy water-soluble polyphenols of alkali, the fluffy drink of the alkali of trace element and abundant amino acid
Material, not only rich in antioxidant contents such as the fluffy middle Polyphenols of alkali, but also has the essential trace elements of the human body and abundant amino acid, Ke Yiman
Pursuit of the sufficient people to nutrition and health care double effects, while adapt to the fast pace life of present people.Extracting solution rich in calcium, magnesium,
The mineral elements such as iron, manganese, zinc, copper, strontium, sodium, and containing abundant amino acid classes, up to 18 kinds, including necessary amino
Acid：Threonine, valine, isoleucine, methionine, leucine, phenylalanine, lysine, tryptophan.Nonessential amino acid：
Asparatate, serine, glutamic acid, glycine, alanine, cystine, tyrosine, histidine, arginine, proline.Its
Middle essential amino acids content accounts for total amino acid content difference 35.2%, the reference model (36%) proposed close to FAO, it is necessary to amino acid
Ratio with nonessential amino acid is 55%, the reference model (0.56) proposed close to FAO.This shows Suaeda liaotungensis kitag green plant
Amino acid content and the reference model that is provided close to FAO (Food and Agriculture Organization of the United Nation) of composition, there is Development volue.
5th, the fluffy polyphenols recovery rate of alkali may be up to 5.9% in the present invention, and polyphenols is referred to as " the 7th class nutrition
Element ", polyphenol can promote lipid and bile acid to exclude external, the content of cholesterol and triglycerides in reduction blood plasma and liver, tool
There is prevention of arterial hardening, reduce blood pressure and blood fat, also the prevention effect such as thrombus, very strong antioxidation, have and delay to decline
Always, the function such as pre- anti-cancer, suitable diabetic, disease in the liver and gallbladder patient, hypertension, cardiovascular patient, intestines and stomach disease
Patient, old man and child overweight people drink, and have especially for the elderly, diabetic, hypertensive patient notable
Health-care effect, helps health.
6th, the fluffy raw material of alkali chooses alkali fluffy plant of the growth cycle within 45 days in the present invention, and the fluffy cauline leaf of alkali at this time is fresh
It is tender, rich in nutritional ingredients such as functional pigmented, vitamin, protein and alkaloids, in addition, the fluffy raw material of alkali selected by the present invention
For artificial cultivation, wild suaeda glauce raw material heavy metals exceeding standard problem that may be present, artificial cultivation, good growing environment are avoided
It ensure that the security of raw material.
7th, sucrose is free of in the fluffy beverage of alkali of the present invention, pure plant extracting solution is green safe, diabetic food
With.
8th, physical and chemical index detection is carried out to the fluffy beverage of alkali provided by the invention, every leading indicator is satisfied by national standard will
Ask.
【Brief description of the drawings】
Fig. 1 is influence of the liquid-solid ratio to the fluffy pigment extraction effect of alkali；
Fig. 2 is the response surface that solid-liquid ratio, cellulase, vitamin C and ultrasonic power influence polyphenol content.
【Embodiment】
The present invention can be explained in more detail by the following examples, the open purpose of the present invention is intended to the protection present invention
In the range of all changes and improvements, the invention is not limited in the following examples；
A kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, includes the following steps：
Step 1, fresh Leaves of Suaeda Salsa is gathered, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, the material after crushing is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1:
Add water at 20~1: 50, adds 2%~4% (w/w) cellulase, adds 0.05%~0.08% vitamin C, enzymolysis time
20min~30min, 30 DEG C~50 DEG C, ultrasonic power 50w~200w of hydrolysis temperature, extraction time are 1~2 time, merge extraction
Liquid；
Step 3, leaching liquor carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the water in step 1 used in wet pulverizing, which calculates, to exist
In step 2 in 1: 20~1: 50 feed liquid mass ratio.
Preferably, water used in step 1 and step 2 is deionized water, and the mineral in water have been filtered out in deionized water
Matter, avoids the interference of calcium in water, magnesium plasma.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the wet pulverizing technique in step 1 can be replaced
Dry pulverization process, is specially：Fresh Leaves of Suaeda Salsa is gathered, food purifying machine is put into and cleans 1 time~3 times, utilize vegetable drying
Sterilization machine is dried, and temperature is 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is spare to cross 20~40 mesh sieves.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the leaching liquor its mineral described in step 2
Matter component and content are as follows：Ca2+μ g/L of 2985 μ g/L~5200, Mg2+μ g/L of 1743 μ g/L~2791, Fe2+162 μ g/L~185
μg/L、Mn2+μ g/L of 5.11 μ g/L~8.03, Zn+μ g/L of 5.27 μ g/L~8.51, K+μ g/L of 0.8 μ g/L~3.0, Na+1746μg/
The μ g/L of μ g/L of L~6899,200 μ g/L of total dissolved solid~400.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, the reverse osmosis unit system configuration described in step 3
For：Pretreatment system, smart filtration system, corollary system；Pretreatment system is filtered by mechanical filter, adsorption filter, security personnel
Device forms；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system is made of water purification pot, electrical control system.
The method that the ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, ultrafiltration described in step 3 are hollow-fibre membrane ultrafiltration
To remove the microorganisms such as bacterium in extracting solution, mould, a diameter of 0.01 μm~0.001 μm of the hollow-fibre membrane, feed rate
For 900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration is the μ g/L of 200 μ g/L~400, the ultrafiltration time
100min~400min, backwash cycle 10min~40min, backwash time 20min~120min, upper operation 10min~40min,
Upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.
Wherein, step 1 can save energy consumption using wet pulverizing, the techniques such as drying in dry pulverization process be avoided, beneficial to energy saving.
Addition vitamin C can play the color protection fluffy to alkali, anti-oxidation efficacy in step 2, while can also realize battalion
Support the purpose strengthened.
Embodiment 1
(1) fresh Leaves of Suaeda Salsa is gathered, after cleaning 1 time, 30 DEG C of drying, crush, it is spare to cross 20 mesh sieves；
(2) by feed liquid mass ratio 1: 20 plus deionized water, 2% (w/w) cellulase is added, adds 0.05% vitamin C,
Enzymolysis time 20min, 35 DEG C, ultrasonic power 50w of hydrolysis temperature, extraction time are 2 times, merge leaching liquor twice, successively
Filtered by mechanical filter, adsorption filter, cartridge filter；
(3) and then using a diameter of 0.001 μm of hollow-fibre membrane ultrafiltration injection gas-water mixer, feed liquor speed is 900L/
H, pressure 0.045Mpa, feed concentration are 200 μ g/L, and ultrafiltration time 200min, backwash cycle 20min, backwash time 40s, is adopted
With operation 20min, upper backwash 40s modes, filtrate sterile filling both obtained.
Embodiment 2
(1) fresh Leaves of Suaeda Salsa is gathered, after cleaning 2 times, 40 DEG C of drying, crush, it is spare to cross 30 mesh sieves；
(2) by feed liquid mass ratio 1: 30 plus deionized water, 2.5% (w/w) cellulase is added, adds 0.06% vitamin
C, enzymolysis time 25min, 40 DEG C, ultrasonic power 100w of hydrolysis temperature, extraction time are 2 times, merge leaching liquor twice, according to
It is secondary to be filtered by mechanical filter, adsorption filter, cartridge filter；
(3) and then using a diameter of 0.004 μm of hollow-fibre membrane ultrafiltration injection gas-water mixer, feed liquor speed is 1100L/
H, pressure 0.06Mpa, feed concentration are 300 μ g/L, and ultrafiltration time 200min, backwash cycle 30min, backwash time 60s, use
Upper operation 30min, upper backwash 60s modes, filtrate sterile filling both obtained.
Embodiment 3
(1) fresh Leaves of Suaeda Salsa is gathered, after cleaning 3 times, 40 DEG C of drying, crush, it is spare to cross 30 mesh sieves；
(2) by feed liquid mass ratio 1: 40 plus deionized water, 4% (w/w) cellulase is added, adds 0.07% vitamin C,
Enzymolysis time 30min, 45 DEG C, ultrasonic power 150w of hydrolysis temperature, extraction time are 2 times, merge leaching liquor twice, successively
Filtered by mechanical filter, adsorption filter, cartridge filter；
(3) and then using a diameter of 0.007 μm of hollow-fibre membrane ultrafiltration injection gas-water mixer, feed liquor speed is 1300L/
H, pressure 0.1Mpa, feed concentration are 300 μ g/L, and ultrafiltration time 200min, backwash cycle 20min, backwash time 60s, use
Upper operation 20min, lower backwash 60s modes, filtrate sterile filling both obtained.
Embodiment 4
(1) fresh Leaves of Suaeda Salsa is gathered, after cleaning 3 times, 50 DEG C of drying, crush, it is spare to cross 40 mesh sieves；
(2) by feed liquid mass ratio 1: 50 plus deionized water, 4% (w/w) cellulase is added, adds 0.08% vitamin C,
Enzymolysis time 40min, 50 DEG C, ultrasonic power 200w of hydrolysis temperature, extraction time are 2 times, merge leaching liquor twice, successively
Filtered by mechanical filter, adsorption filter, cartridge filter；
(3) and then using a diameter of 0.01 μm of hollow-fibre membrane ultrafiltration injection gas-water mixer, feed liquor speed is 1500L/
H, pressure 0.18Mpa, feed concentration are 400 μ g/L, and ultrafiltration time 200min, backwash cycle 20min, backwash time 40s, use
Upper operation 20min, lower backwash 40s modes.Filtrate sterile filling both obtained.
The detection of every physical and chemical index：
1~4 preparation method of Example prepare the fluffy beverage of alkali, by GB/T12143.1, GB8537-1995,
GB4789 examination criterias compare indices, the results are shown in Table 1.
The fluffy beverage indices of 1 alkali of table
It is satisfied by by the visible fluffy beverage items leading indicator of alkali prepared according to 1~4 method of the embodiment of the present invention of 1 result of table
Requirements of the national standard.
The fluffy leaching liquor Mineral Elements in Jadeite Shellfish content such as table 2 below of alkali in the present invention：
The fluffy leaching liquor Mineral Elements in Jadeite Shellfish content of 2 alkali of table
Experimental study is carried out with the solid-liquid ratio of water for alkali is fluffy, is drawn with the solid-liquid ratio of water, the present invention in order to determine that alkali is fluffy
Liquid-solid ratio is to the influence figure of the fluffy pigment extraction effect of alkali, as shown in Figure 1, as shown in Figure 1, as the increase of liquid-solid ratio, pigment are molten
The absorbance of liquid gradually rises, and when liquid-solid ratio is 40: 1, light absorption value significantly raises, and show that the fluffy pigment recovery rate of alkali also significantly rises
Height, when liquid-solid ratio is more than 40: 1, curve tends towards stability, and absorbance changes less, considers energy-saving and emission-reduction, selects liquid-solid ratio
For 40: 1 (mL/g).
On the basis of above-mentioned single factor experiment, 4 solid-liquid ratio, cellulase, vitamin C and ultrasonic power factors are chosen
Box-Behnken designs are carried out, the results are shown in Table 3：
3 Box-Behnken experimental designs scheme of table and result
Regression fit is carried out to 3 test data of table using 8.0 design softwares of Design-Expert, obtains polyphenol content pair
Solid-liquid ratio, cellulase, the secondary multinomial regression model of 4 factors of vitamin C and ultrasonic power：
Polyphenol content=5.55+0.13A+0.19B+0.32C+0.19D-0.33A2-0.39B2-0.28C2-0.96D2
4 response surface design quadratic regression equation model the results of analysis of variance of table
Note：* significant difference p ＜ 0.05；The extremely notable p ＜ 0.01 of * differences.
Response surface figure is made according to regression equation, investigates the shape of be fitted response surface, analysis solid-liquid ratio, cellulase,
Fig. 2 is met personally in the influence of vitamin C and ultrasonic power to polyphenol content, its response.As shown in Table 4, mutation model p value ＜
0.0001, reach the pole level of signifiance, illustrate that the models fitting degree is fine.As shown in Figure 2, it is 1: 50 in solid-liquid ratio, cellulose
Enzyme 3%, vitamin C 0.05%, ultrasonic power 125w, polyphenol content reach peak, and the result and experiment of single factor
As a result it is consistent.
It is above-mentioned test result indicates that, the present invention is aided in by ultrasonic wave, aqueous enzymatic extraction and ultrafiltration technology triple combination pole
It is big remain alkali it is fluffy in water miscible functional components, the fluffy middle polyphenols recovery rate of alkali carries compared to traditional extraction process
Rate is taken to improve 30%-70%, the fluffy polyphenol recovery rate of alkali of the invention may be up to 5.9%, and the fluffy beverage of alkali produced by the present invention was both
Rich in antioxidant contents such as the fluffy middle Polyphenols of alkali, and there are the essential trace elements of the human body and abundant amino acid, can meet people
Pursuit to nutrition and health care double effects, while adapt to the fast pace life of present people, be of great significance.
Part not in the detailed description of the invention is the prior art.
Claims (7)
1. a kind of method that ultrasound wave auxiliary enzyme method prepares the fluffy beverage of alkali, it is characterized in that：Include the following steps：
Step 1, fresh Leaves of Suaeda Salsa is gathered, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, the material after crushing is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1: 20~
1: 50 plus water, 2%~4% (w/w) cellulase is added, adds 0.05%~0.08% vitamin C, enzymolysis time 20min~
30min, 30 DEG C~50 DEG C, ultrasonic power 50w~200w of hydrolysis temperature, extraction time are 1~2 time, merge leaching liquor；
Step 3, leaching liquor carries out coarse filtration, ultrafiltration by reverse osmosis unit, and sterile filling to obtain the final product.
2. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：It is wet in step 1
Method crushes water used and calculates in step 2 in 1: 20~1: 50 feed liquid mass ratio.
3. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：Step 1 and step
Water used is deionized water in rapid 2.
4. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：In step 1
Wet pulverizing technique can be replaced dry pulverization process, be specially：Fresh Leaves of Suaeda Salsa is gathered, is put into food purifying machine cleaning 1
All over~3 times, dried using vegetable drying sterilization machine, temperature is 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is standby to cross 20~40 mesh sieves
With.
5. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：Described in step 2
Its mineral composition of the leaching liquor and content it is as follows：Ca2+μ g/L of 2985 μ g/L~5200, Mg2+1743 μ g/L~2791
μg/L、Fe2+μ g/L of 162 μ g/L~185, Mn2+μ g/L of 5.11 μ g/L~8.03, Zn+μ g/L of 5.27 μ g/L~8.51, K+0.8μg/
μ g/L of L~3.0, Na+The μ g/L of μ g/L of 1746 μ g/L~6899,200 μ g/L of total dissolved solid~400.
6. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：Described in step 3
Reverse osmosis unit system configuration be：Pretreatment system, smart filtration system, corollary system；Pretreatment system by mechanical filter,
Adsorption filter, cartridge filter composition；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system by water purification pot,
Electrical control system forms.
7. the method that ultrasound wave auxiliary enzyme method according to claim 1 prepares the fluffy beverage of alkali, it is characterized in that：Described in step 3
Ultrafiltration is hollow-fibre membrane ultrafiltration to remove the microorganisms such as bacterium in extracting solution, mould, a diameter of 0.01 μ of hollow-fibre membrane
M~0.001 μm, feed rate are 900L/h~1500L/h, and pressure 0.045Mpa~0.18Mpa, feed concentration is 200 μ g/L
~400 μ g/L, ultrafiltration time 100min~400min, backwash cycle 10min~40min, backwash time 20min~120min,
Upper operation 10min~40min, upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.
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| CN108740650A (en) * | 2018-05-27 | 2018-11-06 | 辽宁泓新科技成果转化服务有限公司 | A kind of fluffy sead extract beverage of alkali and preparation method thereof |
| CN109527312A (en) * | 2018-12-14 | 2019-03-29 | 山东蓝碳科技有限公司 | Functional marine biology typical local food adds composition, beverage and preparation method thereof |
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| CN102987490A (en) * | 2012-12-18 | 2013-03-27 | 山东绿洲醇食品有限公司 | Suaeda glauca wild vegetable juice and production method and application thereof |
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| CN106666640A (en) * | 2017-01-04 | 2017-05-17 | 杭州博可生物科技有限公司 | Natural plant nutritional salt capable of replacing sodium chloride and process for preparing natural plant nutritional salt |
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