CN109805404A - A kind of preparation method and application of the fluffy extract of alkali - Google Patents
A kind of preparation method and application of the fluffy extract of alkali Download PDFInfo
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Abstract
A kind of preparation method and application of the fluffy extract of alkali, it is related to a kind of extracting method, it is combined by ultrasonic wave with dynamic countercurrent circulation, so that the fluffy middle polyphenol recovery rate of alkali greatly improves, it plays a multiplier effect, ultrasonic wave auxiliary, aqueous enzymatic extraction process integration greatly remain alkali it is fluffy in water-soluble functional components, the fluffy middle polyphenols recovery rate of alkali improves 30%-70% compared to traditional extraction process recovery rate, the fluffy polyphenol recovery rate of alkali of the invention may be up to 5.9%, the fluffy polyphenol extract of alkali can be applied to preparation cardiovascular disease, hypertension, hyperlipidemia, diabetes, liver and bladder disease, beautifying face and moistering lotion, the health food or health drink of anticancer.
Description
[technical field]
The invention belongs to the preparation method and applications of plant extract development field more particularly to a kind of fluffy extract of alkali.
[background technique]
Fluffy alkali is annual herb plant, is generally born on the saline-alkali wastelands such as beach, lakeside, river valley, is up in salt content
3% intertidal zone can also be grown, and be a kind of typical salt-soda soil indicator plant.Alkali is fluffy to be had ecological protection to soil and improves work
With being known as " pionner " of alkaline land improvement.The study found that alkali it is fluffy fresh and tender cauline leaf it is full of nutrition, rich in fat, albumen
Matter, crude fibre, minerals, microelement and multivitamin etc., great Development volue.Suaeda salsa is annual herb salification
Plant, the fluffy middle nutritional ingredient of alkali is complete and enriches, and the fluffy stem leaf protein matter of alkali, fat, carbohydrate three nutritious elements content are equal
Higher, seed fat content accounts for the 36.5% of dry matter, is higher than soybean, and the protein content of fresh and tender cauline leaf accounts for dry matter
40%, 16 kinds of amino acid are measured altogether in Suaeda salsa, wherein there are 8 kinds of essential amino acids, and content is higher, there is expansion blood
Manage, enhance human immunity, being depressured, hypoglycemic, prevention and cure of cardiovascular disease the effect of；Also containing there are many needed by human micro- during alkali is fluffy
Secondary element and vitamin abundant, wherein calcium, magnesium, zinc, iron, nickel content are abundant, and calcium, phosphorus, iron and core yellow cellulose content are much higher than
The vegetables such as spinach, tomato and carrot, Vitamin C content are higher than or are equivalent to general vegetables, and vitamin B content is general vegetables
5-8 times, be much higher than spirulina；Invention Announce number is that the patent document of CN106578918A discloses a kind of fluffy nutrition of selenium-rich alkali
Healthy fine dried noodles and its production technology, patent document research demonstrate fluffy middle high 10 times of the more general food of Se content of alkali or so, and
Selenium is referred to as " king of anti-cancer " in body trace element by scientist, with anticancer, pre- preventing tumor, the generation of hepatopathy, raising
The effect of body immunity；Invention Announce number are as follows: the patent document of CN103027962A discloses a kind of Suaeda salsa flavones
Extraction and content assaying method, it is higher that patent document research demonstrates Suaeda salsa (S.salsa) leaf flavonoids content, and has
Stronger antioxidant activity, plant flavone have the multiple efficacies such as anti-oxidant, antitumor, antiatherosclerosis, anti-inflammatory.
The one kind of pigment as polyphenol, the pigment during alkali is fluffy are the valuable sources of natural pigment, and the pigment is through researching and analysing
For water-soluble anthocyanidin.Anthocyanidin has anti-mutation, resists as natural pigment, security performance with higher
The healthcare functions such as oxidation, blood pressure lowering, protection liver, belong to functional food additives.Polyphenols is referred to as " the 7th class battalion
Support element ".Polyphenol is natural " antioxidant ", possesses superpower oxidation resistance, can prevent body from aoxidizing, and can remove human body
The free radical of generation, delaying skin aging；The decomposition for inhibiting collagen, plays crease-resistant function.Moreover, it or it is good
" anticarcinogen " inhibits the activation of carcinogen, resists cell cancerization.
It is external that polyphenol can promote lipid and bile acid to exclude, and reduces containing for cholesterol and triglycerides in blood plasma and liver
Amount has the effects that prevention of arterial hardening, reduces blood pressure and blood lipid, prevention and treatment thrombus, and there are also very strong antioxidations, have and prolong
The functions such as slow aging, pre- anti-cancer.
Antioxidant activity is analysis shows alkali is fluffy to O-2Free radical has stronger scavenging effect, often edible to be conducive to remove body
Interior excessive oxygen radical, to be good for one's health and long-lived.The fluffy edible vegetables ancient as one kind of alkali have higher simultaneously
Medical value, according to the introduction of supplementary Amplifications of the Compendium of Materia Medica, salty cool, the nontoxic, heat-clearing of property, disperse accumulation.Modern medicine study is also sent out
Existing, Suaeda plant has the effects that hypoglycemic, decompression, expands blood vessel, prevents and treats heart disease and enhance human immunity, and is suitable for the heart
The prevention of vascular system disease has significant health-care effect especially for the elderly, hypertensive patient.However alkali is fluffy at present
Processing and eating method are all in the primary stage, before the fluffy superior biological nature of alkali and effect determine its wide application
Scape, research and development have very big economic value and social value.
CN 201510166655.0 discloses a kind of method of water extract method production fluffy beverage of health food alkali, and acquisition is new
Fresh Suaeda salsa aubergine blade, dries in the shade after cleaning repeatedly, crushes, and crosses 30 meshes；According to feed liquid mass ratio 1:25~30, water
Extraction 2 times, merges leaching liquor twice, filters；According to leaching liquor: the mass ratio of water be 1:1, mass percentage 12%~
15% white granulated sugar, the citric acid of mass percentage 0.04%~0.06%, the honey of mass percentage 3%~5%, matter
Amount percentage 0.05%~0.08% Vc be uniformly mixed to get.Temperature 50 C~60 DEG C of the flooding, time
50~60min carries out pasteurize after mixing, and the method is high pol there are problem, and Consumer groups are limited, unfavorable strong
Health.Secondly, water-soluble polyphenols substance recovery rate is low, and pasteurize can destroy anthocyan material stability, cannot retain
Its functional characteristic.
CN 201510398358.9 discloses a kind of processing method of mineral water, including the following steps: 1) by natural mine
Substance is put into container after being crushed to 0.1~0.5 centimetre of little particle washing；2) it gives in container by weight the pure of injection 2 to 1
Water purification；3) it again to the ozone for being passed through 0.3~10 hour in container, sets only five minutes；4) it will finally be set only from the water outlet of container
Water outflow afterwards.Natural mineral matter described in the method refers to the jade of the composition containing minerals, with single nutrient component, only rich
Containing minerals.
CN 201510467632.3 discloses a kind of Suaeda salsa beverage, and by weight percentage, the beverage includes following
Component: Suaeda salsa 8%~10%, glucose 10%~15%, purified water 70%~75%, sodium bicarbonate 2%~5% and right
Nipagin A 1%~3%.The invention presses juice using dissection, obtains stoste and solid slag, and solid slag is added water immersion and is added
Heat obtains the fluffy juice of alkali, and using boiling, cooling, the techniques such as sterilizing, the fluffy juice of the alkali that the method obtains passes through the links such as high temperature sterilization,
The stability of vitamin substances is seriously destroyed, and product is added to a large amount of glucide, is unfavorable for health.
In conclusion alkali it is fluffy have all multi-nutrients, have good health-care efficacy, have great economic value with
Social value, and there are no a kind of methods of energy fluffy middle polyphenol substance of high efficiency extraction alkali on Vehicles Collected from Market, also contain day without a kind of
The beverage of right plant polyphenol, minerals and amino acid.
[summary of the invention]
In order to overcome the shortcomings in the background art, the present invention develops a kind of preparation method and application of fluffy extract of alkali,
It is combined by ultrasonic wave with dynamic countercurrent circulation, so that the fluffy middle polyphenol recovery rate of alkali greatly improves, plays the effect got twice the result with half the effort
Fruit, furthermore ultrasonic wave auxiliary, aqueous enzymatic extraction process integration greatly remain alkali it is fluffy in water-soluble functional components, in turn
A kind of fluffy polyphenol extract of alkali is made.
For achieving the above object, the present invention adopts the following technical scheme:
A kind of preparation method of the fluffy extract of alkali, it is characterized in that: including the following steps:
Step 1, fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1:
20~1:50 adds water, adds 2%~4% (w/w) cellulase, and 0.05%~0.08% vitamin C, enzymolysis time is added
20min~30min, 30 DEG C~50 DEG C of hydrolysis temperature, ultrasonic power 50w~200w, extraction time is 1~2 time, merges extraction
Liquid；
Step 3, leaching liquor obtains the fluffy polyphenol extract of alkali by reverse osmosis unit progress coarse filtration, ultrafiltration.
The application of the fluffy polyphenol extract of alkali is applied to preparation cardiovascular disease, hypertension, hyperlipidemia, diabetes, liver and gallbladder
Disease, beautifying face and moistering lotion, anticancer health food.
The application of the fluffy polyphenol extract of alkali is applied to preparation cardiovascular disease, hypertension, hyperlipidemia, diabetes, liver and gallbladder
Disease, beautifying face and moistering lotion, anticancer health drink.
The preparation method of the fluffy extract of the alkali, in step 1 water used in wet pulverizing calculate in step 2 1:20~
In the feed liquid mass ratio of 1:50.
The preparation method of the fluffy extract of the alkali, water used in step 1 and step 2 are deionized water.
The preparation method of the fluffy extract of the alkali, the wet pulverizing technique in step 1 can be replaced dry pulverization process, specifically
Are as follows: fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, dried using vegetable drying sterilization machine, temperature
Degree is 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is spare to cross 20~40 meshes.
The preparation method of the fluffy extract of the alkali, described its mineral composition of leaching liquor and content described in step 2
It is as follows: Ca2+2985 μ g/L of μ g/L~5200, Mg2+1743 μ g/L of μ g/L~2791, Fe2+162 μ g/L of μ g/L~185, Mn2+5.11
μ g/L of μ g/L~8.03, Zn+5.27 μ g/L of μ g/L~8.51, K+0.8 μ g/L of μ g/L~3.0, Na+1746 μ g/L of μ g/L~6899,
200 μ of μ g/L~400 g/L of total dissolved solid.
The preparation method of the fluffy extract of the alkali, reverse osmosis unit system configuration described in step 3 are as follows: pretreatment system
System, smart filtration system, corollary system；Pretreatment system is made of mechanical filter, adsorption filter, cartridge filter；Smart mistake
Filter system is made of hollow-fibre ultrafiltration device；Corollary system is made of water purification pot, electrical control system.
The preparation method of the fluffy extract of the alkali, it is characterized in that: ultrafiltration described in step 3 is hollow-fibre membrane ultrafiltration to go
Except the microorganisms such as bacterium, mould in extracting solution, the hollow-fibre membrane diameter is 0.01 μm~0.001 μm, and feed rate is
900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration are the 200 μ g/L of μ g/L~400, ultrafiltration time
100min~400min, backwash cycle 10min~40min, backwash time 20min~120min, upper operation 10min~40min,
Upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.
Due to the adoption of the technical scheme as described above, the invention has the following beneficial effects:
1, the present invention is combined by ultrasonic wave with dynamic countercurrent circulation, so that the fluffy middle polyphenol recovery rate of alkali greatly improves,
It plays a multiplier effect；
2, the present invention by ultrasonic wave assist, aqueous enzymatic extraction process integration greatly remain alkali it is fluffy in water-soluble function
Energy property ingredient, the fluffy middle polyphenols recovery rate of alkali improve 30%-70%, aqueous enzymatic method compared to traditional extraction process recovery rate
In conjunction with ultrasonic wave physical process, the properties such as the very big acceleration generated using it, strong cavitation effect, high vibration are reduced
It is asked when extraction, temperature is avoided to change the activity change to effective component；Ultrasonic wave dynamic countercurrent auxiliary enzymes of the invention and super
Filtering technology combine extract the fluffy middle polyphenols of alkali method simple production process, expend the time it is few, reduce production work it is strong
Degree has many advantages, such as that product effective component is high, reduces temperature and changes bring adverse effect.
3, the present invention using coarse filtration and ultrafiltration effectively eliminate the impurity such as body refuse, suspended matter, algae in extracting solution and
The microorganisms such as bacterium, mould in extracting solution, the various nutritional ingredients and microelement for remaining the fluffy extracting solution of alkali of high degree,
It is easy to operate, it is suitable for industrialized production, compared to traditional filter method, hyperfiltration technique is a kind of purification for being widely used in water,
Pollution-free, low energy consumption, and separator is simple, process is short, easy to operate, easily controllable and maintenance, installation area are small.Normal
Temperature is lower to be carried out, and mild condition is without component damage, thus the separation and enrichment of the particularly suitable substance to this product (thermo-responsive), right
The recycling of micro constitutent in this product is highly effective.Mechanical filter filtering can go to extract in ultrafiltration technology pretreatment system
Body refuse and fiber in liquid etc., after through adsorption filter filtering can remove the impurity such as oil removal and algae, and then pass through
The hollow-fibre membrane ultrafiltration of ultrafiltration technology essence filtration system is to remove the microorganisms such as bacterium in extracting solution, mould.
4, the fluffy polyphenol extract of alkali produced by the present invention is rich in the fluffy water-soluble polyphenols of alkali, microelement and abundant amino acid
The fluffy beverage of alkali not only rich in antioxidant contents such as the fluffy middle Polyphenols of alkali, but also has the essential trace elements of the human body and amino acid abundant,
It can satisfy pursuit of the people to nutrition and health care double effects, while adapting to the fast pace life of present people.Extracting solution is rich in
Mineral elements such as calcium, magnesium, iron, manganese, zinc, copper, strontium, sodium, and containing abundant amino acid classes, up to 18 kinds, including must
Palpus amino acid: threonine, valine, isoleucine, methionine, leucine, phenylalanine, lysine, tryptophan.Nonessential ammonia
Base acid: asparatate, serine, glutamic acid, glycine, alanine, cystine, tyrosine, histidine, arginine, dried meat ammonia
Acid.Wherein essential amino acids content accounts for total amino acid content difference 35.2%, the reference model (36%) proposed close to FAO, it is necessary to
The ratio of amino acid and nonessential amino acid is 55%, the reference model (0.56) proposed close to FAO.This shows that Suaeda liaotungensis kitag is green
The reference model that the amino acid content and composition of color plant are provided close to FAO (Food and Agriculture Organization of the United Nation) has Development volue.
5, the fluffy polyphenols recovery rate of alkali may be up to 5.9% in the present invention, and polyphenols is referred to as " the 7th class nutrition
Element ", it is external that polyphenol can promote lipid and bile acid to exclude, and reduces the content of cholesterol and triglycerides in blood plasma and liver, tool
There is the effects of prevention of arterial hardening, reduce blood pressure with blood lipid, prevention and treatment thrombus, there are also very strong antioxidations, have and delay to decline
Always, polyphenol extract can be applied to diabetic, disease in the liver and gallbladder patient, hypertension, cardiac blood by the functions such as pre- anti-cancer
In pipe patient, enterogastric diseases patient, old man and child overweight people's edible food or beverage, especially for the elderly, sugar
Patient, hypertensive patient are urinated with significant health-care effect, help health.
6, the fluffy raw material of alkali chooses alkali fluffy plant of the growth cycle within 45 days in the present invention, and the fluffy cauline leaf of alkali at this time is fresh
It is tender, rich in nutritional ingredients such as functional pigmented, vitamin, protein and alkaloids, in addition, the fluffy raw material of alkali selected by the present invention
For artificial cultivation, wild suaeda glauce raw material heavy metals exceeding standard problem that may be present, artificial cultivation, good growing environment are avoided
It ensure that the safety of raw material.
[Detailed description of the invention]
Fig. 1 is influence of the liquid-solid ratio to the fluffy pigment extraction effect of alkali；
Fig. 2 is the response surface that solid-liquid ratio, cellulase, vitamin C and ultrasonic power influence polyphenol content.
[specific embodiment]
The present invention can be explained in more detail by the following examples, the open purpose of the present invention is intended to protect the present invention
All changes and improvements in range, the invention is not limited to the following examples；
A kind of preparation method of the fluffy extract of alkali, it is characterized in that: including the following steps:
Step 1, fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, by feed liquid mass ratio 1:
20~1:50 adds water, adds 2%~4% (w/w) cellulase, and 0.05%~0.08% vitamin C, enzymolysis time is added
20min~30min, 30 DEG C~50 DEG C of hydrolysis temperature, ultrasonic power 50w~200w, extraction time is 1~2 time, merges extraction
Liquid；
Step 3, leaching liquor obtains the fluffy polyphenol extract of alkali by reverse osmosis unit progress coarse filtration, ultrafiltration.
The application of the fluffy polyphenol extract of alkali is applied to preparation cardiovascular disease, hypertension, hyperlipidemia, diabetes, liver and gallbladder
Disease, beautifying face and moistering lotion, anticancer health food.
The application of the fluffy polyphenol extract of alkali is applied to preparation cardiovascular disease, hypertension, hyperlipidemia, diabetes, liver and gallbladder
Disease, beautifying face and moistering lotion, anticancer health drink.
The preparation method of the fluffy extract of the alkali, in step 1 water used in wet pulverizing calculate in step 2 1:20~
In the feed liquid mass ratio of 1:50.
The preparation method of the fluffy extract of the alkali, water used in step 1 and step 2 are deionized water.
The preparation method of the fluffy extract of the alkali, the wet pulverizing technique in step 1 can be replaced dry pulverization process, specifically
Are as follows: fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, dried using vegetable drying sterilization machine, temperature
Degree is 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is spare to cross 20~40 meshes.
The preparation method of the fluffy extract of the alkali, described its mineral composition of leaching liquor and content described in step 2
It is as follows: Ca2+2985 μ g/L of μ g/L~5200, Mg2+1743 μ g/L of μ g/L~2791, Fe2+162 μ g/L of μ g/L~185, Mn2+5.11
μ g/L of μ g/L~8.03, Zn+5.27 μ g/L of μ g/L~8.51, K+0.8 μ g/L of μ g/L~3.0, Na+1746 μ g/L of μ g/L~6899,
200 μ of μ g/L~400 g/L of total dissolved solid.
The preparation method of the fluffy extract of the alkali, reverse osmosis unit system configuration described in step 3 are as follows: pretreatment system
System, smart filtration system, corollary system；Pretreatment system is made of mechanical filter, adsorption filter, cartridge filter；Smart mistake
Filter system is made of hollow-fibre ultrafiltration device；Corollary system is made of water purification pot, electrical control system.
The preparation method of the fluffy extract of the alkali, it is characterized in that: ultrafiltration described in step 3 is hollow-fibre membrane ultrafiltration to go
Except the microorganisms such as bacterium, mould in extracting solution, the hollow-fibre membrane diameter is 0.01 μm~0.001 μm, and feed rate is
900L/h~1500L/h, pressure 0.045Mpa~0.18Mpa, feed concentration are the 200 μ g/L of μ g/L~400, ultrafiltration time
100min~400min, backwash cycle 10min~40min, backwash time 20min~120min, upper operation 10min~40min,
Upper backwash 20s~120s, lower operation 10min~40min, lower backwash 20s~120s.
Wherein, step 1 can save energy consumption using wet pulverizing, avoid the techniques such as the drying in dry pulverization process, be conducive to energy conservation.
Addition vitamin C can play the color protection fluffy to alkali, anti-oxidation efficacy in step 2, while also can be realized battalion
Support the purpose strengthened.
Embodiment 1
(1) fresh Leaves of Suaeda Salsa is acquired, after cleaning 1 time, 30 DEG C of drying crush, it is spare to cross 20 meshes；
(2) add deionized water by feed liquid mass ratio 1:20,2% (w/w) cellulase be added, 0.05% vitamin C is added,
Enzymolysis time 20min, 35 DEG C of hydrolysis temperature, ultrasonic power 50w, extraction time is 2 times, merges leaching liquor twice, successively
It is filtered by mechanical filter, adsorption filter, cartridge filter；
(3) diameter is then used to inject gas-water mixer for 0.001 μm of hollow-fibre membrane ultrafiltration, feed liquor rate is 900L/
H, pressure 0.045Mpa, feed concentration are 200 μ g/L, ultrafiltration time 200min, backwash cycle 20min, and backwash time 40s is adopted
With operation 20min, upper backwash 40s mode.
Embodiment 2
(1) fresh Leaves of Suaeda Salsa is acquired, after cleaning 2 times, 40 DEG C of drying crush, it is spare to cross 30 meshes；
(2) add deionized water by feed liquid mass ratio 1:30,2.5% (w/w) cellulase is added, 0.06% vitamin is added
C, enzymolysis time 25min, 40 DEG C of hydrolysis temperature, ultrasonic power 100w, extraction time is 2 times, merges leaching liquor twice, according to
It is secondary to be filtered by mechanical filter, adsorption filter, cartridge filter；
(3) diameter is then used to inject gas-water mixer for 0.004 μm of hollow-fibre membrane ultrafiltration, feed liquor rate is 1100L/
H, pressure 0.06Mpa, feed concentration are 300 μ g/L, ultrafiltration time 200min, backwash cycle 30min, and backwash time 60s is used
Upper operation 30min, upper backwash 60s mode.
Embodiment 3
(1) fresh Leaves of Suaeda Salsa is acquired, after cleaning 3 times, 40 DEG C of drying crush, it is spare to cross 30 meshes；
(2) add deionized water by feed liquid mass ratio 1:40,4% (w/w) cellulase be added, 0.07% vitamin C is added,
Enzymolysis time 30min, 45 DEG C of hydrolysis temperature, ultrasonic power 150w, extraction time is 2 times, merges leaching liquor twice, successively
It is filtered by mechanical filter, adsorption filter, cartridge filter；
(3) diameter is then used to inject gas-water mixer for 0.007 μm of hollow-fibre membrane ultrafiltration, feed liquor rate is 1300L/
H, pressure 0.1Mpa, feed concentration are 300 μ g/L, ultrafiltration time 200min, backwash cycle 20min, and backwash time 60s is used
Upper operation 20min, lower backwash 60s mode.
Embodiment 4
(1) fresh Leaves of Suaeda Salsa is acquired, after cleaning 3 times, 50 DEG C of drying crush, it is spare to cross 40 meshes；
(2) add deionized water by feed liquid mass ratio 1:50,4% (w/w) cellulase be added, 0.08% vitamin C is added,
Enzymolysis time 40min, 50 DEG C of hydrolysis temperature, ultrasonic power 200w, extraction time is 2 times, merges leaching liquor twice, successively
It is filtered by mechanical filter, adsorption filter, cartridge filter；
(3) diameter is then used to inject gas-water mixer for 0.01 μm of hollow-fibre membrane ultrafiltration, feed liquor rate is 1500L/
H, pressure 0.18Mpa, feed concentration are 400 μ g/L, ultrafiltration time 200min, backwash cycle 20min, and backwash time 40s is used
Upper operation 20min, lower backwash 40s mode.
The detection of every physical and chemical index:
Fluffy leaching liquor Mineral Elements in Jadeite Shellfish content such as the following table 1 of alkali in the present invention:
The fluffy leaching liquor Mineral Elements in Jadeite Shellfish content of 1 alkali of table
In order to determine the fluffy solid-liquid ratio with water of alkali, the present invention carries out experimental study for the fluffy solid-liquid ratio with water of alkali, obtains
Liquid-solid ratio is to the influence diagram of the fluffy pigment extraction effect of alkali, as shown in Figure 1, as shown in Figure 1, with the increase of liquid-solid ratio, pigment is molten
The absorbance of liquid gradually rises, and when liquid-solid ratio is 40:1, light absorption value is significantly increased, and show that the fluffy pigment recovery rate of alkali also significantly rises
Height, when liquid-solid ratio is greater than 40:1, curve tends towards stability, and absorbance changes less, comprehensively considers energy-saving and emission-reduction, selects liquid-solid ratio
For 40:1 (mL/g).
On the basis of above-mentioned single factor experiment, solid-liquid ratio, 4 cellulase, vitamin C and ultrasonic power factors are chosen
Box-Behnken design is carried out, the results are shown in Table 2:
2 Box-Behnken experimental design scheme of table and result
Regression fit is carried out to 2 test data of table using 8.0 design software of Design-Expert, obtains polyphenol content pair
Solid-liquid ratio, cellulase, 4 factors of vitamin C and ultrasonic power secondary multinomial regression model:
Polyphenol content=5.55+0.13A+0.19B+0.32C+0.19D-0.33A2-0.39B2-0.28C2-0.96D2
3 response surface design quadratic regression equation model the results of analysis of variance of table
Note: * significant difference p < 0.05；The extremely significant p < 0.01 of * difference.
Make response surface figure according to regression equation, investigate the shape of be fitted response surface, analysis solid-liquid ratio, cellulase,
Fig. 2 is met personally in the influence of vitamin C and ultrasonic power to polyphenol content, response.As shown in Table 3, mutation model p value < 0.0001,
Reach extremely significant level, illustrates that the models fitting degree is fine.It as shown in Figure 2, is 1:50, cellulase 3%, dimension in solid-liquid ratio
Raw element C 0.05%, ultrasonic power 125w, polyphenol content reach peak, and the result and experiment of single factor result one
It causes.
It is above-mentioned the experimental results showed that, the present invention is assisted by ultrasonic wave, aqueous enzymatic extraction process integration greatly remains
Water-soluble functional components during alkali is fluffy, the fluffy middle polyphenols recovery rate of alkali are improved compared to traditional extraction process recovery rate
30%-70%, the fluffy polyphenol recovery rate of alkali of the invention may be up to 5.9%, and the fluffy polyphenol extract of alkali can be applied to preparation painstaking effort
Pipe disease, hypertension, hyperlipidemia, diabetes, liver and bladder disease, beautifying face and moistering lotion, the health food of anticancer or health drink.
Part not in the detailed description of the invention is the prior art.
Claims (9)
1. a kind of preparation method of the fluffy extract of alkali, it is characterized in that: including the following steps:
Step 1, fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, carry out wet pulverizing；
Step 2, smashed material is put into ultrasonic wave dynamic countercurrent circulation extracting concentrating equipment, pressed
Feed liquid mass ratio 1:20~1:50 adds water, adds 2%~4%(w/w) cellulase, 0.05%~0.08% vitamin C is added,
The enzymolysis time min of 20 min~30,30 DEG C~50 DEG C of hydrolysis temperature, the ultrasonic power w of 50 w~200, extraction time be 1~
2 times, merge leaching liquor；
Step 3, leaching liquor obtains the fluffy polyphenol extract of alkali by reverse osmosis unit progress coarse filtration, ultrafiltration.
2. the application of the fluffy polyphenol extract of alkali according to claim 1, it is characterised in that: be applied to preparation cardiovascular disease, height
Blood pressure, hyperlipidemia, diabetes, liver and bladder disease, beautifying face and moistering lotion, anticancer health food.
3. the application of the fluffy polyphenol extract of the alkali according to claim 1, it is characterised in that: be applied to preparation cardiovascular disease, height
Blood pressure, hyperlipidemia, diabetes, liver and bladder disease, beautifying face and moistering lotion, anticancer health drink.
4. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: in step 1 used in wet pulverizing
Water calculates in the feed liquid mass ratio of 1:20~1:50 in step 2.
5. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: water used in step 1 and step 2
For deionized water.
6. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: the wet pulverizing technique in step 1
It can be replaced dry pulverization process, specifically: fresh Leaves of Suaeda Salsa is acquired, food purifying machine is put into and cleans 1 time~3 times, utilize
The drying of vegetable drying sterilization machine, temperature are 30 DEG C~50 DEG C, and fruits and vegetables pulverizer crushes, and it is spare to cross 20~40 meshes.
7. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: extraction described described in step 2
Its mineral composition of liquid and content are as follows: Ca2+ 2985 μ g/L of μ g/L~5200, Mg2+1743 μ g/L of μ g/L~2791,
Fe2+ 162 μ g/L of μ g/L~185, Mn2+ 5.11 μ g/L of μ g/L~8.03, Zn+ 5.27 μ g/L of μ g/L~8.51, K+
0.8 μ g/L of μ g/L~3.0, Na+ 1746 μ g/L of μ g/L~6899,200 μ of μ g/L~400 g/L of total dissolved solid.
8. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: reverse osmosis unit described in step 3
System configuration are as follows: pretreatment system, smart filtration system, corollary system；Pretreatment system by mechanical filter, adsorption filter,
Cartridge filter composition；Smart filtration system is made of hollow-fibre ultrafiltration device；Corollary system is by water purification pot, electrical control system
Composition.
9. the preparation method of the fluffy extract of alkali according to claim 1, it is characterized in that: ultrafiltration described in step 3 is hollow fibre
To remove the microorganisms such as bacterium in extracting solution, mould, the hollow-fibre membrane diameter is 0.01 μm~0.001 μ for dimension film ultrafiltration
M, feed rate are the L/h of 900 L/h~1500, and the pressure Mpa of 0.045 Mpa~0.18, feed concentration is 200 g/L~400 μ
μ g/L, ultrafiltration time 100 min~400 min, the backwash cycle min of 10 min~40, backwash time 20 min~120 min,
Upper operation 10 min~40 min, upper backwash 20 s~120 s, lower operation 10 min~40 min, 20 s~120 of lower backwash
s。
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