CN109790547B - 控制植物中气孔关闭的构建体和方法 - Google Patents

控制植物中气孔关闭的构建体和方法 Download PDF

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CN109790547B
CN109790547B CN201780048261.2A CN201780048261A CN109790547B CN 109790547 B CN109790547 B CN 109790547B CN 201780048261 A CN201780048261 A CN 201780048261A CN 109790547 B CN109790547 B CN 109790547B
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N.莱昂哈特
L.马丁
S.基亚伦扎
H.杰奎特
L.努索梅
M.贾韦尔
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Abstract

本发明涉及在植物中表达AHA5蛋白质，优选地是导致AHA5的组成型活性的突变的AHA5蛋白质，以控制植物中的气孔关闭并改善对干旱条件的耐受性和提高产量。

Description

控制植物中气孔关闭的构建体和方法
背景
干旱是对于在当今农业区之外进行作物扩张的最大限制因素之一。由于公认的全球气候的变化，这在曾经该问题被忽视的地区将变得越来越重要。今天，人们担心改善干旱易发地区的栽培措施和作物基因型。
在植物中，大部分水分损失通过叶子表面上的孔隙发生，这被称为气孔。叶子中气孔的大小是可变的，并控制水蒸气从植物中扩散的速率。除了控制水分流失外，气孔还允许CO2扩散到叶子中进行光合作用。因此，气孔永久性地控制碳吸收和水分损失之间的权衡。通过保卫细胞响应环境刺激和应激条件对气孔运动进行调节是决定作物植物水分利用效率和生产率的主要因素。
在植物中，初级离子泵H+-ATP酶是质膜的主要蛋白质。利用ATP的化学能，质膜H+-ATP酶从植物细胞中排出质子以生成电化学质子梯度。该梯度在提供用于跨质膜的次级活性转运的能量方面起主要作用。分子研究已显示，植物H+-ATP酶由多基因家族编码。值得注意的是，已经在拟南芥基因组中鉴定了命名为AHA1至AHA11的11种同种型(Axelsen等，2001)。该家族成员之间的比对已表明它们具有至少66％的蛋白质序列同一性(Axelsen等，2001)。它们具有100个氨基酸长的C末端结构域的共同结构，称为R(调节)或自抑制结构域，包含14-3-3结合位点和倒数第二个Thr残基处的磷酸化位点。
拟南芥质膜H+-ATP酶(AHA)家族成员的表达谱的概述可见于Genevestigator数据库(www.genevestigator.org)。两种基因，AHA1和AHA2几乎在所有组织和器官中表达。因此，这些基因似乎起到离子稳态所需的管家基因的作用。在芽中发现相对更多的AHA1转录物，而AHA2主要在根中表达，特别是在根毛中。AHA3，AHA4和AHA11在整个植物中也表现出广泛的表达，但未表达为相同的程度。根据报告基因分析研究，当AHA4在根内胚层中具有高表达时，可见一定程度的特化(Vitart等，2001)。实时RT-PCR分析证实AHA1，AHA2，AHA3和AHA11是在叶中发现的主要转录物(Alsterfjord等，2004)。报告基因分析显示AHA3启动子在叶韧皮部伴侣细胞中具有活性(DeWitt等，1991)。AHA6和AHA9(主要在花药中表达)，AHA7和AHA8(在花粉中几乎为排他性表达)和AHA10(长角果中的最高转录物水平)的表达模式表明由这些基因编码的泵具有更特化的功能。报告基因分析证实了AHA9在花药中的表达(Houlne等，1994)和AHA10在发育中的种皮内皮中的表达(Baxter等，2005)。发现AHA5主要在保卫细胞中表达(Ueno等人，2005)。在拟南芥保卫细胞中，可以检测到所有11个H+-ATP酶同基因的表达，但是AHA1，AHA2和AHA5是主要的同种型(Ueno等，2005)。
植物质膜H+-ATP酶在不同的生理功能中起重要作用，包括养分吸收，细胞内pH调节和气孔开放(Haruta等，2015)。除了调节生理过程外，质膜质子泵还在植物对不断变化的条件的适应中具有作用。因此，H+-ATP酶可以是在应激条件下活化的抗性机制的共有元件。
为了确定植物内不同同种型的功能，对于11个基因中的10个，已经在植物模型拟南芥中获得了单个功能丧失突变体。然而，可能是由于功能冗余，这些突变体在正常生长条件下没有表现出表型。例如，aha1和aha2敲低的突变体在标准条件下正常生长，而双纯合突变体是胚胎致死的(Haruta等，2010)。这清楚地说明了这两种蛋白质对植物生理学至关重要。此外，使用生理遗传筛选，已经鉴定了两个拟南芥ost2(open stomata2)突变体，并且OST2基因座的定位克隆显示其与已知的编码质膜H+-ATPase的AHA1基因的同一性(Merlot等，2007)。OST2的两个显性的功能改变的等位基因分别被鉴定为编码AHA1-P68S和AHA1-L169F-G867S泵变体。两种突变都破坏了AHA1泵的自动抑制调节并导致其组成型活性。有趣的是，这种泵失调诱导了多效性作用，包括叶片自发坏死的发展与水杨酸含量(SA)的增加，根中H+排出(酸化)的增加相关，但其也是气孔的异常控制，导致了叶片失水异常高。
一些研究表明，几个残基处的置换导致活化的H+-ATP酶，包括在ost2突变体中检测到的AHA1中的两个置换。实际上，包括W875L，G867L或P68S的AHA1的突变体形式随着分子活性的增加而升高至高亲和力H+-ATP酶(Baunsgaard等，1996；Merlot等，2007)。使用基于酵母互补的功能测定，这些置换显示出在其内源性质膜PMA1中破坏的酵母突变体RS-72中的致死生长缺陷，但不是其野生型计数器(Cid等，1987；Baunsgaard等，1996；Merlot等，2007)。此外，梅洛等人(2007)教导了AHA1参与了植物中气孔开度的调节，并且与野生型相比，两种ost2突变体的气孔更加开放，尤其是在黑暗条件下。
有许多不同的文件描述了控制气孔关闭的机制。然而，它们都没有提供改善耐旱性的解决方案。
在EP2995680中，发明人在保卫细胞特异性启动子的控制下在拟南芥植物中过表达AHA2。他们观察到气孔在转化的植物中更开放，促进光合作用并可能提高产量。AHA2促进气孔的开放，因此本领域技术人员不会认为该基因在改善植物的耐旱性方面是令人关注的。
WO2014012145旨在通过过表达AHA1来抑制气孔关闭以增加蒸腾作用。当试图改善耐旱性时，本领域技术人员不会考虑该文献。
来自拟南芥和玉米的AHA5的序列列于EP1033405、US20130074202和US20090217414的序列表中。然而，没有迹象表明可能会使用AHA5。
在US8577624中描述了与ZmAHA5接近的蛋白质。该专利涉及鉴定III型P型ATP酶的潜在抑制剂的方法，产生这种调节剂的方法和实施本发明的计算机辅助方法。
对于开发用于控制气孔关闭以改善植物的耐旱性和用于植物有效利用水的解决方案仍存在很大兴趣。
发明内容
本发明在于在植物中表达AHA5蛋白，并且优选地是导致AHA5的组成型活性的突变的AHA5蛋白，以控制植物中的气孔关闭和改善对干旱条件的耐受性。
本发明基于植物的转化的惊人效果，所述植物利用编码AHA5蛋白的核酸和优选地导致AHA5的组成型活性的突变的AHA5蛋白进行转化，产生相较于相应的非转基因植物对干旱胁迫具有提高的耐受性的转基因植物。
因此，本发明包括(i)过表达野生型AHA5蛋白，或(ii)表达或过表达导致AHA5的组成型活性的突变的AHA5蛋白，用于控制气孔关闭和改善植物对干旱条件的耐受性。本发明还在于通过气孔关闭获得耐旱植物的方法。
在第一方面，本发明涉及控制植物中气孔关闭的方法，所述方法包括表达或过表达AHA5蛋白。所述方法允许通过控制由于AHA5蛋白活性引起的气孔关闭来赋予耐旱性或抗旱性。
优选地，通过过表达编码突变的AHA5的核酸获得AHA5的表达或过表达，所述突变的AHA5导致AHA5蛋白的组成型活性。
在第二方面，本发明涉及鉴定具有封闭气孔的植物的方法，包括在植物群体中鉴定过表达AHA5蛋白质或具有组成型活性的AHA5的植物的步骤，特别是过表达野生型AHA5蛋白或组成型活性AHA5蛋白的植物。
在另一方面，本发明还涉及一种培育植物的方法，包括播种植物种子的步骤，其中所述植物种子来源于表达或过表达AHA5蛋白的植物，以及从这些播种的种子中培育植物的步骤，其中在干旱/缺水胁迫条件下进行生长阶段。
在又一方面，本发明涉及编码组成型活性AHA5蛋白的核酸序列，该核酸序列优选地具有遗传修饰，该遗传修饰导致AHA5蛋白的第一跨膜区段中、或AHA5蛋白的自身抑制性C末端中的R1结构域中的氨基酸修饰，或AHA5蛋白的自抑制性C末端中的R1结构域的至少部分的缺失，和更优选具有导致AHA5蛋白的第一跨膜区段或自抑制性C末端中的R1结构域中的氨基酸置换的遗传修饰。
在另一方面，本发明还涉及核酸构建体，其包含在植物中有功能的启动子，并且使得其至少在保卫细胞中表达，其与编码根据本发明的AHA5蛋白的核酸序列可操作地连接。
本发明的其他方面是包含核酸构建体的转基因植物，所述核酸构建体包含在植物中有功能的启动子，并且使得其至少在保卫细胞中表达，所述构建体与编码组成型活性AHA5蛋白的核酸序列可操作地连接，或所述构建体包含编码野生型AHA5蛋白的核酸，该核酸与使得其至少在保卫细胞中表达的启动子连接，且其中所述启动子并非编码所述野生型AHA5蛋白质的核酸的内源性启动子。
本发明的又一方面提供了通过表达或过表达AHA5蛋白来减少植物中水分流失的方法。
在又一另外的方面，本发明提供了一种在非胁迫或胁迫条件下增加植物产量以供水或至少在干旱/缺水胁迫条件下维持植物产量的方法，所述方法包括在非胁迫条件或胁迫条件下培育过表达野生型AHA5蛋白或组成型活性的AHA5蛋白的转基因植物的步骤以用于供水或干旱/缺水条件。
适当地，减少水分损失或增加植物产量的方法包括，优选地，通过AHA5蛋白的第一跨膜区段或自抑制性C末端的R1结构域中的氨基酸置换表达或过表达组成型活性AHA5蛋白。
在与控制气孔关闭和改善植物对干旱条件的耐受性相关的所有这些方面中，本发明包括通过在植物中(i)过表达野生型AHA5蛋白，或(ii)表达或过表达导致组成型活性的AHA5蛋白的突变的AHA5蛋白及其方法。
附图简述
图1是拟南芥AHA5(AtAHA5)野生型蛋白质(931个氨基酸)的示意图，其具有不同的功能结构域(TM＝跨膜结构域；自抑制性C末端中的R1结构域)。获得组成型活性AHA5的氨基酸置换的三个位置表示为：P50S，G849S，W857L。
图2：酵母突变体RS-72的互补，其中编码H+-ATP酶PMA1的必需基因在半乳糖(GAL)诱导型启动子的严格控制下被置换。
图3：用pAHA5:EGFP-GUS构建体转化的植物(Col)中AtAHA5基因的表达谱。
图4：用pSbMYB60:ZsGreen构建体转化的植物(玉米)中SbMYB60基因的表达谱。比例尺，20μm。
图5：在35S启动子控制下用AtAHA5、AtAHA5P50S或AtAHA5W857L转化的植物中的失水实验(OE表示过表达)。
图6：在pZmRAB17启动子控制下在用ZmAHA5W885L转化的玉米幼苗中测量的相较于A188对照的三个事件#A，#B，#C的白天的水分损失实验(A)和动力学(B)。
图7：在35S启动子控制下用AtAHA5、AtAHA5P50S或AtAHA5W857L转化的植物中响应于光的气孔运动的测量结果(OE表示过表达)。
图8：在35S启动子控制下用AtAHA5、AtAHA5P50S或AtAHA5W857L转化的植物中莲座鲜重的测量结果(OE表示过表达)。
图9：在充分浇水(WW)或水胁迫(WS)条件下生长的转基因玉米幼苗的第四片叶中，在启动子pSbMYB60的控制下表达的ZsGreen的相对表达。
图10：在充分浇水(WW)或水胁迫(WS)条件下生长的转基因玉米植物的第十片叶和旗叶中，在启动子pSbMYB60的控制下表达的ZsGreen的相对表达。
图11：在充分浇水(WW)或水胁迫(WS)条件下生长的转基因玉米植物的第十片叶中的ZsGreen荧光的共聚焦显微镜成像。比例尺，10μm。
发明详述
本发明基于以下事实：AHA5蛋白或组成型活性的AHA5蛋白的过表达或表达在植物中控制气孔关闭，其赋予耐旱性，从而导致产量的改善或至少不变。
根据本发明，植物中的气孔关闭可以通过过表达野生型AHA5蛋白，通过过表达组成型活性的突变的AHA5蛋白，或通过直接在所述植物中表达组成型活性AHA5来控制。
在第一方面，本发明涉及在植物中控制气孔关闭的方法，所述方法包括表达或过表达AHA5蛋白，优选过表达AHA5蛋白。
在一个实施方案中，在包括AHA5蛋白的过表达的植物中控制气孔关闭的方法是包括过表达野生型AHA5蛋白或组成型活性AHA5蛋白的方法。它可以通过用于植物中的任何常规的过表达方法来获得，例如用包含编码AHA5蛋白的所述核酸序列的载体进行转化。所述转化可以用细菌菌株如根癌农杆菌(Agrobacterium tumefaciens)来进行或通过直接方法如电穿孔、基因枪轰击或本领域技术人员已知的其他方法进行。优选地，通过用包含在植物中起作用的启动子和编码AHA5蛋白的核酸序列的载体转化植物来过表达AHA5蛋白，并再生所述细胞以获得完整植物。所述载体可以通过根癌土壤杆菌引入植物中。尤其是，可以使用Ishida等人(1996)描述的用于单子叶植物的转化的方法。
在另一个实施方案中，控制植物中气孔关闭的方法包括直接在植物中表达组成型活性的AHA5蛋白。根据本申请中公开的突变，它可以通过基因编辑技术直接突变植物细胞中的基因而获得，例如，CRISPR/Cas9或TALEN。这些技术也是技术人员所熟知的。
如本文所用的术语“气孔闭合”或“闭合的气孔”都指完全关闭的气孔和部分闭合的气孔。根据本发明，当两个保卫细胞之间的孔径与给定植物中的开放气孔相比减少至少50％时，气孔被认为是闭合的或部分闭合的。当植物置于光照条件和无水胁迫条件下时，认为气孔是开放的。特别地，在本发明的一个优选实施方案中，当孔径减少至少50％，至少60％，至少70％，至少80％和高达85％时，认为气孔是部分闭合的。在另一个优选的实施方案中，与开口气孔相比，当孔径减小超过85％时，认为气孔是闭合的。
气孔闭合或孔径可以通过本领域技术人员已知的任何经典方法测量。
例如，气孔闭合或孔径可以通过直接方法测量，例如但不限于可视化测量，特别是使用显微镜检查并在光照或黑暗条件下测量两个保卫细胞之间的孔径(leonhardt N等人；1997年)。
气孔闭合或孔径也可以通过基于参数测量的间接方法进行测量，例如但不限于：(i)莲座叶片近轴表面温度，特别是使用热成像，例如通过红外热成像(Merlot S等人，2002)，
(ii)通道活动，例如钾或钙通道，特别是使用对保卫细胞原生质体的膜片钳实验(Perfus-Barbeoch L等，2002)，
(iii)气孔导度，特别是叶片使用分析手持式气孔计，监测气体交换的阻力，例如通过测量叶片温度和相对湿度(Clément M等，2011)，或使用气体交换测量室，
(iv)蒸腾速率，特别是基于植物重量损失除以植物叶面积(Caldeira等，2014)
根据一个优选的实施方案，控制气孔关闭的方法包括至少在保卫细胞中表达或过表达AHA5蛋白，更优选至少在保卫细胞中具有组成型活性的突变AHA5蛋白的过表达。
本文所用的表述“控制气孔关闭”或“气孔关闭的控制”是指作用于气孔关闭，特别是促进气孔关闭。
在一个实施方案中，控制气孔关闭的方法包括在植物中过表达野生型AHA5蛋白。
如本文所用，术语“野生型”是指天然存在于至少一种给定生物体中并且未被人类改变，修饰或突变的氨基酸序列。
所述野生型AHA5蛋白可以来自任何植物(属，种)，比如拟南芥(SEQ ID NO:1)，玉米(SEQ ID NO:2)，向日葵(Helianthus annuus)(SEQ ID NO:3和SEQ ID NO:4)，番茄(Lycopersicon esculentum)(SEQ ID NO:5)，獐茅(Aeluropus littoralis)(SEQ ID NO:6和SEQ ID NO:7)，高粱(Sorghum bicolor)(SEQ ID NO:8)，谷子(Setaria italica)(SEQID NO:9)，水稻(Oryza sativa)(SEQ ID NO:10)，二穗短柄草(Brachypodium distachyon)(SEQ ID NO:11)，大麦(Hordeum vulgare)(SEQ ID NO:12和SEQ ID NO:13)，小麦(Triticum aestivum)(SEQ ID NO:14、SEQ ID NO:15和SEQ ID NO:16)，红花荠菜(Capsella rubella)(SEQ ID NO:17)，芥兰(Camelina sativa)(SEQ ID NO:18)，蒺藜状苜蓿(Medicago truncatula)(SEQ ID NO:19)，胡杨(Populus euphratica)(SEQ ID NO:20)。
在一个优选的实施方案中，所述野生型AHA5蛋白质选自SEQ ID NO:1和SEQ IDNO:2所示的序列。
在另一个实施方案中，并且其代表一个优选的实施方案，用于控制气孔关闭的方法包括突变的AHA5的表达或过表达，其导致AHA5蛋白的组成型活性。
术语“组成型活性AHA5”或“组成型AHA5活性”是指突变的AHA5H+-ATP酶，以便始终具有活性。这可以通过例如AHA5蛋白的第一跨膜结构域或R1调节结构域中的氨基酸修饰或通过缺失AHA5蛋白的自抑制性C末端中R1结构域的至少部分来获得。优选地，组成型AHA5活性可以通过AHA5蛋白的第一跨膜区段或R1调节结构域中的氨基酸置换来获得。
在一个实施方案中，根据本发明的控制气孔关闭的方法包括组成型活性AHA5蛋白的表达或过表达，所述组成型活性AHA5蛋白是在第一跨膜结构域中突变的AHA5蛋白。
如本文所用，第一跨膜结构域在于(consist in)23个氨基酸的共有序列FLGFMWNPLSWVMEX1AAX2MAIAX3(SEQ ID NO:21)，其中：
X1:M，V，A
X2:L，I，V
X3：L，M
优选地，控制气孔关闭的方法包括参照上述共有序列(SEQ ID NO:21)在第一跨膜结构域中用氨基酸S置换第8位的氨基酸P而突变的AHA5蛋白的表达或过表达FLGFMWNSLSWVMEX1AAX2MAIAX3(SEQ ID NO:22)。
在一个优选的实施方案中，控制气孔关闭的方法包括表达或过表达来自拟南芥的突变的AHA5蛋白，如SEQ ID NO:23所示。
在另一个实施方案中，根据本发明的控制气孔关闭的方法包括组成型活性AHA5蛋白的表达或过表达，所述组成型活性AHA5蛋白是通过R1结构域中的氨基酸置换或缺失修饰的AHA5蛋白。
如本文所用，R1结构域在于107个氨基酸的共有序列
LSGX4AWX5NX6LX7NKX8AFTX9KX10X11YGX12X13EREAQWAX14AQRTLHGLQX15X16EX17X18X19X2 0X21X22FX23X24KX25SYX26X27LSX28IAEQAKRRAEX29X30RLREX31X32X33LKX34HVESVVKLKGLDIX35TIX36QX37YTV(SEQ ID NO：24-27)，
其中：
X5：D，L，N，R
X6：L，M
X7：E，Q，D
X8：T，I
X9：T，S
X10：K，E
X11：D，N
X12：K，R
X13：E，G
X14：T，A，L
X15：P，A
X16：P，A
X17：T，V，A，P，K，S
X18：无氨基酸，A，S，N
X19：无氨基酸，S，T，N
X20：N，S，H
X21：N，T
X22：I，V，M，L
X23：N，S，H
X24：E，D
X25：S，N
X26：R，S，H
X27：E，D
X28：E，Q
X29：I，V，M
X30：A，V
X31：L，I，V
X32：N，L，H
X33：T，S
X34：G，S
X35：D，E
X36：Q，N
X37：N，H
优选地，控制气孔关闭的方法包括参照上述共有序列SEQ ID NO:24-27，表达或过表达在R1结构域的以下位置的一个或多个位点，更优选一个位点突变的AHA5蛋白：
·22，其中氨基酸Y被A置换，或；
·23，其中氨基酸G被S或A置换，或；
·24，其中氨基酸K或R被A置换，或；
·27，其中氨基酸R被A置换，或；
·31，其中氨基酸W被L或A置换，或；
·32，其中氨基酸A被S置换，或；
·35，其中氨基酸Q被A置换，或；
·36，其中氨基酸R被A置换，或；
·41，其中氨基酸L被A置换，或；
·68，其中氨基酸A被S或T置换，或；
·70，其中氨基酸R被A置换，或；
·71，其中氨基酸R被A置换，或；
·74，其中氨基酸I被A置换
在上述置换中，下列位置中的一个或多个，优选一个是优选的：
·22，其中氨基酸Y被A置换，或；
·23，其中氨基酸G被S或A置换，或；
·24，其中氨基酸K或R被A置换，或；
·23，其中氨基酸G被S或A置换，或；
·31，其中氨基酸W被L或A置换，或；
·36，其中氨基酸R被A置换，或；
·41，其中氨基酸L被A置换，或；
·69，其中氨基酸R被A置换，或；
用于获得组成型活性AHA5蛋白的最优选的置换在31位，其中氨基酸W被L或A置换。因此，在本发明的一个有利的实施方案中，控制气孔关闭的方法包括表达或过表达参照上述共有序列SEQ ID NO:24-27在R1结构域中的第31位突变的AHA5蛋白，其中氨基酸W被L或A置换，
LSGX4AWX5NX6LX7NKX8AFTX9KX10X11YGX12X13EREAQLAX14AQRTLHGLQX15X16EX17X18X19X2 0X21X22FX23X24KX25SYX26X27LSX28IAEQAKRRAEX29X30RLREX31X32X33LKX34HVESVVKLKGLDIX35TIX36QX37YTV(SEQ ID NO：28)或LSGX4AWX5NX6LX7NKX8AFTX9KX10X11YGX12X13EREAQAAX14AQRTLHGLQX1 5X16EX17X18X19X20X21X22FX23X24KX25SYX26X27LSX28IAEQAKRRAEX29X30RLREX31X32X33LKX34HVESVVKLKGLDIX35TIX36QX37YTV(SEQ ID NO：29).
在一个优选的实施方案中，控制气孔关闭的方法包括表达或过表达来自拟南芥的突变的AHA5蛋白，如SEQ ID NO：30所示，或表达或过表达来自玉米的突变的AHA5蛋白，如SEQ ID NO：31所示。
在本发明的另一个实施方案中，控制气孔关闭的方法包括表达或过表达在自抑制性C末端缺少至少部分R1结构域的AHA5蛋白。
在该特定的实施方案中，R1结构域可以是完全缺失的，因此在AHA5蛋白的C末端缺少序列SEQ ID NO：24-27的107个氨基酸。R1结构域也可以是部分缺失的，优选自AHA5蛋白的C末端的至少67个氨基酸，优选至少78个氨基酸，优选至少93个氨基酸，优选至少96个氨基酸缺失。上述位置参照上述R1结构域共有序列SEQ ID NO：24-27。
根据本发明的控制气孔关闭的方法可以用于控制任何类型植物中的气孔关闭，例如：单子叶植物如玉米，小麦，高粱，水稻，大麦，甘蔗，或双子叶植物如向日葵或来自十字花科的植物如拟南芥、油菜籽，芸苔属(Brassica)或来自茄科(Solanaceae)如番茄和马铃薯。
优选地，该方法用于控制单子叶植物中的气孔关闭，和更优选地选自玉米和小麦，最优选为玉米。
在一个具体的实施方案中，本发明的方法用于通过过表达来自拟南芥属，尤其是拟南芥的AHA5蛋白，或通过过表达来自玉米的AHA5蛋白来控制玉米中的气孔关闭。
在第二方面，本发明涉及鉴定具有封闭气孔的植物的方法，包括在植物群体中鉴定过表达AHA5蛋白或具有组成型活性的AHA5蛋白的植物的步骤，特别是过表达野生型AHA5蛋白或组成型活性的AHA5蛋白的植物。
在一个具体的实施方案中，通过使用对转基因特异性的标志物进行鉴定。在该实施方案中，鉴定步骤因此优选在包括对所述植物群体进行基因分型的步骤之后。
在一个具体的实施方案中，鉴定步骤之前是包括从所述群体中的个体提取RNA的步骤。
在一个具体的实施方案中，鉴定步骤之前是包括从所述群体中的个体提取蛋白质的步骤。可以使用本领域已知的用于检测和定量AHA5蛋白水平的任何经典方法，例如Western印迹。
在另一方面，本发明还涉及种植植物的方法，包括以下步骤：
播种植物种子，其中所述植物种子来源于过表达AHA5蛋白的植物，
从这些播种的种子中培育植物，其中生长期在干旱/缺水胁迫条件下进行。
该方法还可包括收获所述植物的步骤。
如本文所用，表述“干旱胁迫”和“缺水胁迫”是同义的条件。术语“干旱胁迫”是指在植物生长中没有正常浇水的条件，其用作非常常见的术语，包括诱导对植物生长和存活具有有害影响的所有种类的非生物胁迫，例如本文所用的“干旱胁迫”，包括诸如土壤缺水，蒸汽压力不足，热应力或光辐射之类的应力。更具体地，术语“干旱”和“缺水”是指环境条件，其中水的量(例如，用于植物生命的降雨量或其他可用水源)小于特定环境的平均水条件，或者可用水的量小于某种植物或在特定环境中生长的植物通常所需的水量。
根据本申请，干旱胁迫位置是，例如其中位点的谷物产量潜力由于干旱胁迫而未达到的位置。
非应力位置例如是商业杂交品种已经达到谷物产量潜力的位置。
干旱胁迫强度可以通过测量干旱胁迫处理(WUE)和用最佳水量灌溉的参考处理之间的产量损失来评估，该参考处理至少等同于作物的最大蒸发蒸腾(ETM)。
通常以-30％的产量损失为目标，干旱位置的共同分布在产率的-10％和-40％之间。
低干旱胁迫位置通常可以是产量损失在0％和多达-20％之间的位置，中等胁迫位置在-20％和多达-30％之间。
目标生长阶段通常可以从抽穗到R2生长期。
在常见的干旱地区，干旱胁迫期可以从V10和R4生长期之间的时期开始持续。
术语“抗旱性”或“耐旱性”是指植物从干旱胁迫期(即，几天或几天没有水)恢复的能力。在本发明的上下文中，干旱耐受性是指，无论应力的强度和持续时间如何，植物实现尽可能接近最佳产量的产量性能的能力。
产量通常定义为作物的经济价值的可测量的产量。这可以根据数量和/或质量来定义。产量直接取决于几个因素，例如，器官的数量和大小，植物结构(例如，分枝数)，种子产量，叶子衰老等。术语“产量”通常是指具有经济价值的可测量产品，通常与特定作物、区域和一段时间相关。单个植物部分根据其数量，大小和/或重量直接影响产量，或实际产量是作物和年份的每平方米的产量，这是通过将总产量(包括收获和评估的产量)除以种植平方米。植物的术语“产量”可以涉及该植物的植物生物量(根和/或枝条生物量)、繁殖器官、和/或繁殖体(比如种子)。产量可以表示为例如q/ha(q表示对应于100kg的公担，ha表示公顷)。
对于本发明，产率可以按如下计算：
o在收获期间，使用联合收割机上的机载设备测量谷物重量和谷物水分。
o然后使用以下公式将粒重标准化为15％的水分：
标准化的谷物重量＝测量的谷物重量x(100-测量的水分(百分比))/85(100-标准化的水分15％)。例如，如果测量的谷物水分为25％，则标准化的谷物重量为：标准化的谷物重量＝测量的谷物重量×75/85。
o然后以常规单位(例如每公顷的公担)表示产量。
在一个具体的实施方案中，根据本发明的培育植物的方法包括播种植物种子的步骤，所述植物种子源自过表达组成型活性AHA5蛋白的植物，并且，优选地，过表达如上定义的组成型活性的AHA5蛋白。
在另一方面，本发明涉及编码组成型活性的AHA5蛋白的核酸序列，所述核酸序列优选具有遗传修饰，该遗传修饰导致如上文SEQ ID NO:21所述的第一跨膜区段中或AHA5蛋白的自抑制性C末端中的R1结构域中的氨基酸修饰，或通过使如上文SEQ ID NO:24-27所述的AHA5的自抑制性C末端中的R1结构域的至少部分缺失产生的氨基酸修饰，且更优选地导致AHA5蛋白的所述第一跨膜区段中或自抑制性C末端中的所述R1结构域中的氨基酸置换。
在一个优选的实施方案中，核酸序列编码在第一跨膜结构域中突变的组成型活性的AHA5蛋白，和在一个更优选的实施方案中，参照以上共有序列(SEQ ID NO:21)，通过用氨基酸S置换第8位上的氨基酸P，在第一跨膜结构域中使AHA5蛋白突变。
在一个最优选的实施方案中，核酸序列编码来自拟南芥的组成型活性AHA5蛋白，所述蛋白由SEQ ID NO:23代表。
在另一个优选的实施方案中，核酸序列编码通过R1结构域中的氨基酸置换或缺失修饰的组成型活性的AHA5蛋白。更优选地，参照共有序列SEQ ID NO:24-27，所述AHA5蛋白在R1结构域中的一个或多个，优选一个以下位置进行突变：
·22，其中氨基酸Y被A置换，或；
·23，其中氨基酸G被S或A置换，或；
·24，其中氨基酸K或R被A置换，或；
·27，其中氨基酸R被A置换，或；
·31，其中氨基酸W被L或A置换，或；
·32，其中氨基酸A被S置换，或；
·35，其中氨基酸Q被A置换，或；
·36，其中氨基酸R被A置换，或；
·41，其中氨基酸L被A置换，或；
·68，其中氨基酸A被S或T置换，或；
·70，其中氨基酸R被A置换，或；
·71，其中氨基酸R被A置换，或；
·74，其中氨基酸I被A置换
在一个甚至更优选的实施方案中，核酸序列编码组成型活性的AHA5蛋白，其参照共有序列SEQ ID NO:24-27，在R1结构域中的一个或多个，优选一个的以下位置发生突变：
·23，其中氨基酸G被S或A置换，或；
·31，其中氨基酸W被L或A置换，或；
·36，其中氨基酸R被A置换，或；
·41，其中氨基酸L被A置换，或；
·69，其中氨基酸R被A置换，或；
在最优选的实施方案中，核酸序列编码在第31位的R1结构域中突变的组成型活性的AHA5蛋白，其中氨基酸W被L或A置换(分别为SEQ ID NO:28和SEQ ID NO:29)。
在一个优选的实施方案中，核酸序列编码来自拟南芥的突变的AHA5蛋白，所述蛋白由SEQ ID NO:30代表，或者核酸编码来自玉米的突变的AHA5蛋白，所述蛋白由SEQ IDNO:31所代表。
在另一个优选的实施方案中，核酸序列编码在自抑制性C末端中缺少至少部分R1结构域的AHA5蛋白。
在另一方面，本发明还涉及核酸构建体，其包含在植物中有功能的启动子并且至少在保卫细胞中表达，其与编码如上定义的组成型活性的AHA5蛋白的核酸序列可操作地连接。
在植物中有功能的启动子是指能够启动植物细胞中核酸分子转录的核苷酸序列。在植物中起作用的启动子可以是，例如，组成型启动子，诱导型启动子，发育调节的启动子或组织特异性启动子，例如叶特异性启动子，种子特异性启动子，保卫细胞特异性启动子等。
根据本发明，核酸构建体包含在植物中有功能的启动子，并且使其至少在气孔的保卫细胞中表达。
本发明的核酸构建体可以包含对保卫细胞特异性的启动子，例如SbMYB60或允许在整个植物中表达的启动子，例如CsvMV启动子，或在叶子中驱动表达的启动子，例如rbcs启动子，或干旱诱导型启动子诸如RAB17。
在另一方面，本发明还涉及核酸构建体，其包含编码与启动子连接的野生型AHA5蛋白的核酸，所述启动子不是编码所述野生型AHA5的核酸的内源性启动子，且其允许至少在保卫细胞中表达。
本发明的所有核酸构建体均是控制气孔关闭的构建体，其含有编码具有内源性或非内源性启动子的组成型活性的AHA5蛋白的核酸序列或含有在非内源性启动子的控制下编码野生型AHA5蛋白的核酸序列。
本发明的另一方面在于包含核酸构建体的转基因植物，所述核酸构建体包含在植物中有功能的启动子，该启动子与编码组成型活性的AHA5蛋白的核酸序列可操作地连接，或在于包含核酸构建体的转基因植物，所述核酸构建体包含与编码野生型AHA5蛋白的核酸序列可操作地连接的启动子，所述启动子是如上所述的非内源性启动子。
关于编码组成型活性的AHA5蛋白的核酸序列的所有上述优选实施方案也适用于根据本发明的核酸构建体。
本发明的又一方面提供了在植物中降低水分损失的方法，该方法通过在所述植物中表达或过表达AHA5蛋白来实现，特别是在所述植物中过表达野生型AHA5蛋白或表达或过表达组成型活性的AHA5蛋白来实现。
在另一方面，本发明提供了一种在供水的非胁迫或胁迫条件下增加植物产量，或至少在干旱/缺水胁迫条件下维持植物产量的方法，所述方法包括在供水的非胁迫条件或胁迫条件下或在干旱/缺水条件下培育过表达野生型AHA5蛋白或组成型活性AHA5蛋白的转基因植物的步骤。
如本文所用，表述“供水的非胁迫条件”是指相对于植物种类和植物生长的特定环境具有正常或足够供水的条件。
本文所述的关于控制植物气孔关闭的AHA5蛋白的表达或过表达的所有优选的实施方案，经必要的变更适用于根据本发明的方法中AHA5蛋白的表达或过表达，以用于降低植物中的水分损失或增加植物产量。
适宜地，降低水分损失或增加植物产量的方法包括组成型活性AHA5蛋白的表达或过表达，其优选是在AHA5蛋白的第一跨膜结构区段或R1调节结构域中通过氨基酸修饰而突变的AHA5，或通过缺失AHA5蛋白的自抑制性C末端中的R1结构域的至少部分而突变的AHA5。更优选地，组成型活性的AHA5蛋白是在AHA5蛋白的第一跨膜区段或R1调节结构域中通过氨基酸置换而突变的AHA5。
根据本发明，植物产量的增加为，其中：
·与从在所述胁迫条件下培育的未过表达AHA5蛋白的植物获得的产量相比，从在所述缺水胁迫条件下培育的所述转基因植物获得的产量增加了，或者
·与从在所述非胁迫条件下培育的未过表达AHA5蛋白的植物获得的产量相比，从在非胁迫条件下培育的所述转基因植物获得的产量增加了，或者
·与在供水的非胁迫条件下培育的所述转基因植物获得的产量相比，从在所述缺水胁迫条件下培育的所述转基因植物获得的产量得以维持。
在另一方面，本发明提供了与SEQ ID NO:40具有至少80％，优选至少85％，更优选至少90％，甚至更优选至少95％的序列同一性的序列的启动子。该启动子能够在植物的保卫细胞中特异性地驱动表达。该启动子也能够由干旱胁迫来诱导，即，是一种干旱胁迫诱导型启动子。通过本领域技术人员已知的任何序列分析方法计算同一性百分比，特别是使用诸如Needleman-Wunsch的算法。在查询序列的整个长度上计算同一性百分比。
在一个优选的实施方案中，启动子是SEQ ID NO:40。
虽然已经参考本发明的特定实施例描述了本发明，但是本领域技术人员应该理解，在不脱离本发明的真实精神和范围的情况下，可以进行各种改变并且可以等同方式进行替换。另外，可以进行许多修改以使特定情况、材料、物质组成、过程、过程步骤适应本发明的目的、精神和范围。所有这些修改都在所附权利要求的范围内。
以下实施例说明了本发明的某些方面。这些实施例决不会限制本发明。
实施例
实施例1参与质膜质子泵H+-ATP酶的活性调节的两个突变的鉴定
AtAHA5基因由基因合成提供者GeneArt合成，并克隆到pMK载体中。随后，使用以下引物通过PCR扩增AtAHA5：
5'-GGATTCTAGAACTAGTATGGAGGAAGTGTTCGAGGAGCTG-3'(SEQ ID NO:32)和
5'-CGGTATCATAAGCTTGTTAAACGGTGTAATGTTGCTGAATCG-3'(SEQ ID NO:33)，并使用In-Fusion策略(Clontech)遵循制造商的说明将其克隆到通过Spel和HindIII切下的pAG425GPD中。然后，为了测试先前在AHA1中鉴定的置换，通过将点突变引入AtAHA5产生AtAHA5变体。在AHA1中，这些突变产生具有增加的分子活性的高亲和力的H+-ATP酶。
使用Site-Directed Mutagenesis试剂盒(Agilent technology)，按照制造商的说明并使用以下引物通过定点诱变将单个氨基酸置换P50S或W857L导入AtAHA5：5'-GGGTTCATGTGGAACTCATTGTCGTGG-3'(SEQ ID NO:34)，和5'-CCACGACAATGAGTTCCACATGAACCC-3'(SEQ ID NO:35)或5'-GGAAAG AG AAG CTCAATTG G CTGCAG CTC-3'(SEQ ID NO:36)和5'-G AG CTG CAGCC AATTG AG CTTCTCTTTCC-3'(SEQ IDNO:37)。
为了评估这些置换的影响，使用内源性质膜ATP酶被破坏的酵母突变体RS-72进行基于酵母互补的功能测定。在RS-72酵母菌株中，酵母H+-ATP酶(PMA1)的天然启动子已经被半乳糖依赖性(GAL1)启动子取代。质粒出生的植物H+-ATP酶的表达处于组成型PMA1启动子的控制之下。因此，当在半乳糖培养基上培养酵母时，酵母和植物H+-ATP酶均表达。当转移到葡萄糖培养基后，仅产生植物H+-ATP酶，并且，为了生长，酵母依赖于植物酶(Cid等，1987)。
表达野生型AtAHA5但没有内源性酵母ATP酶的酵母在葡萄糖培养基上完全不生长，如先前对于AHA1所观察到的(图2)。令人惊讶的是，与AHA1或AHA2相比，氨基酸置换P50S或W857L均不能拯救酵母的生长表型。使用针对来自酵母的膜蛋白上的植物H+-ATP酶的抗体进行Western分析，证实了酵母中AtAHA5蛋白的表达(数据未显示)。
这些结果表明，AHA1和AHA2蛋白中的氨基酸置换P68S和W875L，而不是AHA5中的相应的氨基酸置换P50S和W857L，能够补充RS-72的生长缺陷。
然后将这些变体用于转化拟南芥植物以评估这些蛋白在响应于干旱胁迫时在与生物量评估相关的气孔关闭和失水率中的影响。
实施例2在AtAHA5启动子区域的控制下标记物基因GUS的保卫细胞特异性表达
先前基于RT-PCR的研究表明AtAHA5在气孔中表达(Ueno等，2005，Leonhardt等，1999)。为详细确定AtAHA5表达谱，产生了在推定的AtAHA5启动子控制下表达报告β-葡糖醛酸糖苷酶(GUS)基因的转基因植物。使用引物5'-GGG GAC AAC TTT GTA TAG AAA AGT TGCAAC CAT CAT GAC AAG CGT CTG-3'(SEQ ID NO:38)和5'-GGG GAC TGC TTT TTT GTA CAAACT TGG GGT ATT CGT ACC TAG ACC CAT CAT T-3'(SEQ ID NO:39)，通过PCR对来自拟南芥哥伦比亚生态型(Col)的基因组DNA扩增位于翻译起始密码子上游的基因组区域，长度为2356bp。然后将该2356bp基因组片段克隆到pBGWFS7载体中的EGFP(增强绿色荧光蛋白)和GUS(β-葡糖醛酸糖苷酶)报告基因的上游。将获得的构建体导入拟南芥中，并对得到的转基因品系进行组织学分析以检测报告子表达域。在所有检查的样品中，仅在来自具有气孔的所有植物气生器官的保卫细胞中检测到报告基因表达(图3)。
这些结果表明仅在保卫细胞中检测到GUS和GFP(B，D，E，F)。在根或叶中的维管组织(A，B，C)和叶肉(A)中未检测到GUS活性或EGFP。
实施例3保卫细胞启动子SbMYB60和ZmMYB60的鉴定
使用AtMYB60蛋白质序列，对通过blastp针对拟南芥基因组(TAIR_V9)，水稻基因组(MSU水稻基因组第7版)，玉米基因组(B73 RefGen_v2)和高粱基因组(MIPS1.4)的第一命中(first hits)进行选择并使用seaview4(Gouy M等，(2010))的Muscle模块进行比对。鉴定保守的区块并将其提供给phyML 3.0软件(Guindon S.等，2010)。鉴定出了以下中最接近的同源基因：水稻(LOC_Os11g35390)，甜高粱(Sb05g021820)和玉米(GRMZM2G108959)。分离直至下一个重复区域的上游区域并进行比对以鉴定保守区段和顺式元件。从Sb05g021820上游922bp的区域，在推定的起始密码子上游149bp处鉴定出一个ABRE盒。ABRE盒从ACGTG突变以产生DOF元件AAAAG。为了克隆目的，在推定的起始密码子上游682bp处修饰另外的碱基。类似地，在玉米中，在来自GRMZM2G108959基因上游773bp的区域，在推定的起始密码子上游71bp处鉴定出一个ABRE盒。ABRE盒从ACGTG突变以产生DOF元件AAAAG。为了克隆的目的，在推定的起始密码子上游702bp处修饰了另外的碱基。
将如上所示例的SbMYB60的推定的起始密码子上游的调节区(SEQ ID NO:40)克隆并与报告基因ZsGreen(Clonetech；Matz等，1999)和拟南芥Sac66多腺苷酸化序列融合，并转化到玉米中。通过初级转化体幼苗的共聚焦显微镜验证了ZsGreen荧光蛋白的保卫细胞特异性表达。保卫细胞特异性高粱MYB60启动子允许在保卫细胞中强烈表达。双色高粱(Sorghum bicolor)基因被鉴定为AtMYB60最接近的同源物(Rusconi等，2013)。
通过共聚焦显微镜分析用融合proSMYB60:ZsGreen稳定转化的玉米T0幼苗。如图4所示，在保卫细胞而非副卫细胞中检测到ZsGreen标记基因的荧光，在玉米叶的表皮铺列细胞中也未检测到。
如上所述还鉴定了玉米MYB60启动子(SEQ ID NO:41)。
实施例4在35S启动子下游和AtAHA5启动子下游克隆AtAHA5并转化拟南芥植物
AtAHA5基因由基因合成提供者GeneArt合成，并克隆到pMK载体中。随后通过AscI，PacI裂解切下AtAHA5，并将其克隆到携带双35S启动子(SEQ ID NO:42)的gateway组成型表达载体pMDC32的AscI，PacI位点。然后，分别使用以下引物通过定点诱变将单个氨基酸置换P50S或W857L引入pMDC32：5-GGGTTCATGTGGAACTCATTGTCGTGG-3'(SEQ ID NO:34)和5-CCACGACAATGAGTTCCACATGAACCC-3'(SEQ ID NO:35)或5-GGAAAG AG AAG CTCAATTG G CTGCAG CTC-3'(SEQ ID NO:36)和5-GAG CTG CAGCC AATTG AG CTTCTCTTTCC-3'(SEQ ID NO:37)。
p35S:AtAHA5P50S构建体对应于SEQ ID NO:43，和p35S:AtAHA5W857L构建体对应于SEQ ID NO:44。
还将AtAHA5野生型基因克隆到双35S启动子的下游以制备构建体SEQ ID NO:45。
使用gateway克隆策略将AtAHA5的启动子(SEQ ID NO:46)与EGFP和AtAHA5基因融合到pB7m34G载体中。然后，使用以下引物通过定点诱变将单氨基酸置换W857L引入pB7m34G：5'-G GAAAG AG AAG CTCAATTGG CTG CAG CTC-3'(SEQ ID NO:36)和5'-G AG CTG CAGCCAATTG AG CTTCTCTTTCC-3'(SEQ ID NO:37)。将构建体(SEQ ID NO:47)引入拟南芥中。
还将AtAHA5野生型基因克隆到AtAHA5启动子的下游(SEQ ID NO:48)。
将所有植物转化质粒载体导入根癌土壤杆菌(Agrobacterium tumefaciens)中，然后使用标准方法(Weigel和Glazebrook，2006)将其用于转化属于哥伦比亚生态型或ost2-2D突变体的拟南芥植物。F3纯合植物用于实验。
实施例5-克隆CsVMV，rbcs，ZmRAB17和SbMYB60启动子下游突变的ZmAHA5并在玉米 中转化
具有突变W885L的ZmAHA5编码序列(GRMZM2G006894)(SEQ ID NO:49或SEQ ID NO:50对应于关于变应原性问题的两个优化版本)经基因合成服务提供者对于玉米表达进行密码优化并克隆到pUC57载体(Genscript)。然后通过CsVMV启动子(Verdaguer等，(1996))(SEQ ID NO:51)和水稻肌动蛋白内含子(McElroy等，1990)(SEQ ID NO:52)与拟南芥Sac66多聚腺苷酸化序列(Jenkins等，1999)(SEQ ID NO:53)之间的限制酶消化和连接将突变的ZmAHA5序列克隆到目的二元质粒pBIOS03092中，形成pBIOS03580。(目的载体是pSB12的衍生物(Komari等，(1996))，含有pActin+肌动蛋白内含子选择性标记物-nos polyA嵌合基因，用于选择玉米转化子和HMWG启动子，报告基因ZsGreen(Clonetech；Matz等，1999)和Sac66腺苷酸化序列之间的盒)。
类似地，通过进行限制酶消化和连接，将突变的ZmAHA5与Rbcs启动子(Matsuoka和Sanada，1991)(SEQ ID NO:54)和玉米Rbcs聚腺苷酸化序列(SEQ ID NO:55)连接，目的二元质粒pBIOS03092形成pBIOS03583。
类似地，通过进行限制酶消化和连接，将突变的ZmAHA5与干旱诱导型玉米Rab17启动子(Vilardell等，1991)(SEQ ID NO:56)和玉米Rab17聚腺苷酸化序列(SEQ ID NO:57)连接，且目的二元质粒pBIOS03092形成pBIOS10481。
通过进行限制酶消化和连接，将突变的ZmAHA5与保卫细胞特异性双色高粱MYB60启动子(SEQ ID NO:40)和拟南芥Sac66多腺苷酸化序列(SEQ ID NO:53)连接，且目的二元质粒pBIOS03092形成pBIOS10646。
根据Komari等(1996)将pBIOS03580，pBIOS03583，pBIOS10481和pBIOS10646转移到农杆菌LBA4404_+pSB1中，分别形成菌株T02781，菌株T02784，菌株T10401和菌株T10584。基本上如Ishida等(1996)所述，用这些农杆菌菌株转化玉米栽培品种A188。
分析pCsVMV-ZmAHA5W885L-AtSac66项(SEQ ID NO:58)，pRbcs-ZmAHA5W885L-Rbcs项(SEQ ID NO:59)，pZmRAB17-ZmAHA5W885L-ZmRab17项(SEQ ID NO:60)和pSbMYB60-ZmAHA5W885L-AtSac66项(SEQ ID NO:61)转化的玉米植物表明植物过表达突变的ZmAHA5。
通过定量逆转录聚合酶链反应(q-RT-PCR)验证转基因的过表达。从T0幼苗叶中提取总RNA。
将RNA逆转录到cDNA中；在这些cDNA上定量突变的ZmAHA5表达，并用内源ZmEF1α(GRMZM2G001327)和ZmUbiquitin(GRMZM2G110983)基因表达进行标准化。
实施例6克隆CsVMV，rbcs，ZmRAB17和SbMYB60启动子下游突变的AtAHA5并转化到 玉米中
具有突变W857L(SEQ ID NO:62)的AtAHA5编码序列(AT2G24520)通过基因合成服务提供者对于玉米表达进行密码子优化，并克隆到pUC57载体(Genscript)中。然后通过限制酶消化CsVMV启动子(Verdaguer等(1996))(SEQ ID NO:51)和水稻肌动蛋白内含子(McElroy等1990)(SEQ ID NO:52)与拟南芥Sac66多聚腺苷酸化序列(Jenkins等(1999))(SEQ ID NO:53)并在其之间进行连接将AtAHA5序列克隆到目的二元质粒pBIOS3091，形成pBIOS03579。
类似地，通过在rbcs启动子和玉米rbcs聚腺苷酸化序列(SEQ ID NO:55)之间进行限制酶消化和连接，将突变的AtAHA5与rbcs启动子(SEQ ID NO:54)连接并克隆到目的二元质粒pBIOS03092，形成pBIOS03582。
类似地，通过进行限制酶消化和连接，将突变的AtAHA5与干旱诱导型玉米Rab17启动子(Vilardell等，1991)(SEQ ID NO:56)和玉米Rab17多腺苷酸化序列(SEQ ID NO:57)连接，并连接到目的二元质粒pBIOS03092，形成pBIOS10231。
通过进行限制酶消化和连接，将突变的AtAHA5与保卫细胞特异性双色高粱MYB60启动子(SEQ ID NO:40)和拟南芥Sac66聚腺苷酸化序列(SEQ ID NO:53)连接，并与目的二元质粒pBIOS03092连接，形成pBIOS10648。
将pBIOS03579，pBIOS03582，pBIOS10231和pBIOS10648转移到农杆菌LBA4404(pSB1)中，分别形成菌株T02780，菌株T02783，菌株T10191和菌株T10586。基本上如Ishida等(1996)所述，用这些农杆菌菌株转化玉米栽培品种A188。
分析pCsVMV-AtAHA5W857L-AtSac66项(SEQ ID NO:63)，pRbcs-AtAHA5W857L-ZmRbcs项(SEQ ID NO:64)，pZmRAB17-AtAHA5W857L-ZmRAB17项(SEQ ID NO:65)和pSbMYB60-AtAHA5W857L-AtSac66项(SEQ ID NO:66)转化的玉米植物表明植物过表达突变的AtAHA5。如前所述，通过定量逆转录聚合酶链反应(q-RT-PCR)验证转基因的过表达。
实施例7玉米田间试验
A现场试验
从AHA5转基因玉米品系(pCsVMV-ZmAHA5W885L-AtSac66项(SEQ ID NO:58)，pRbcs-ZmAHA5W885L-Rbcs项(SEQ ID NO:59)，pZmRAB17-ZmAHA5W885L-ZmRab17项(SEQ ID NO:60)，pSbMYB60-ZmAHA5W885L-AtSac66项(SEQ ID NO:61)，pCsVMV-AtAHA5W857L-AtSac66项(SEQ IDNO:63)，pRbcs-AtAHA5W857L-ZmRbcs项(SEQ ID NO:64)，pZmRAB17-AtAHA5W857L-ZmRAB17项(SEQ ID NO:65)，和pSbMYB60-AtAHA5W857L-AtSac66项(SEQ ID NO:66))，根据前述实施例用测试系进行选择。
首先将转化体(T0)植物与A188系杂交，从而产生T1植物。然后将T1植物自花授粉两次，产生T3植物，其是含有转基因的纯合系。然后将这些T3植物与测试系交叉，从而产生杂交体。该杂交体在转化步骤方面处于T4水平，并且对于转基因是杂合的。这些杂交植物用于田间试验。
获得的对照杂交如下：
对照等价物对应于A188系(用于转换的系)和测试系之间的杂交。
按如下计算收率：
在收获期间，使用联合收割机上的机载设备测量谷物重量和谷物水分。
然后使用以下公式将粒重标准化为15％的水分：标准化的粒重＝测量的粒重x(100-测量的水分(作为百分比))/85(其为100-标准化的水分，15％)。
例如，如果测量的谷物水分为25％，则标准化的谷物重量为：标准化的谷粒重量＝测量的谷物重量×75/85。
然后以常规单位(例如每公顷的公担)表示产量。
B-实验设计：
现场试验分为3个不同的位置。
实验块包含4个重复。实验设计是干旱胁迫位置的随机格子块。每个重复包含两个行播小区，每个小区约有多达70株植物，密度为75000株/公顷。
在该实验中使用的对照为如上所述的对照等效物(A188与测试系杂交)。
干旱胁迫位置是由于干旱胁迫而未达到该位置的谷物产量潜力的位置。
非胁迫位置是商业杂交品种已经达到谷物产量潜力的位置。
通过测量干旱胁迫处理(WUE)和用最佳水量灌溉的参考处理之间的产量损失来评估干旱胁迫强度，该参考处理至少等于作物的最大蒸发蒸腾(ETM)。
产量损失为-30％，干旱位置的共同分布在产率的-10％和-40％之间。
低干旱胁迫位置通常是产量损失在0％和高达-20％之间的位置，中等胁迫位置在-20％和高达-30％之间。
目标生长期阶段通常是从抽穗到R2生长期。在常见的干旱地区，干旱胁迫期可以从V10和R4生长期之间的时期开始持续。
实施例8：失水实验
蒸腾水分损失是与耐旱性相关的最重要因素之一。为了评估水分流失的速率，将在35S启动子控制下用AtAHA5，AtAHA5P50S或AtAHA5W857L转化的植物(Col0或ost2-2D)的莲座叶分离，并在45分钟内测量它们的鲜重变化。AtAHA5的活性增加导致切除的莲座叶的水分流失减少。在光照期开始之后至少4小时，切下来自在生长室中的土壤上培育的不同生态型的莲座叶(21℃，70％相对湿度，8小时/16小时光照/黑暗，300μmol.m-2.s-1)，并转移到黑暗中。通过监测切下的莲座叶的鲜重减少来确定水分损失。图5显示了至少3个独立的测量结果的平均值±SE。
这些数据表明，与野生型(图5A)或ost2-2D叶(图5B)相比，过表达AtAHA5的植物的叶片显示出较慢的水分流失速率。并且，氨基酸置换P50S或W857L增强了这种效果，表明AtAHA5质子泵的组成活性允许降低水分损失率。
在启动子pZmRAB17的控制下用ZmAHA5W885L转化的玉米幼苗在受控条件下在生长室中在土壤上生长。5周之后，植物受到水分胁迫，每天通过监测灌溉停止时幼苗鲜重的减少来确定水分损失。结果显示在图6A和6B中。数据显示，与A188未转化的幼苗叶片相比，在启动子pZmRAB17控制下过表达ZmAHA5W885L的幼苗的叶子显示出较慢的水分流失速率。与对照相比，三个事件(事件#A，事件#B，事件#C)呈现出类似的较慢的失水模式。
实施例9：正常条件下或具有ABA时气孔开度的比较
气孔的打开和关闭受环境因素控制，包括光、湿度和CO2浓度，以及植物激素如ABA(脱落酸)。因为AtAHA5过表达系表现出较慢的水分流失速率，所以测试转化植物的气孔运动以与对照(Col0或ost2-2D)进行比较。在生长室土壤中生长的3-4周龄植物的叶子(21℃，70％相对湿度，8小时/16小时光照/黑暗，300μmol.m-2.s-1)对表皮进行气孔孔径测量。在30mM KCl，10mM MES-Tris pH6.0的黑暗中30分钟后，测量气孔孔径。在光诱导的气孔开放实验中，表皮剥离在22℃下在光照(300μl/ml-2.8-1)下孵育3小时，然后测量气孔孔径。呈现的每个值是来自至少三个独立实验的至少60个孔的平均值。误差条代表平均值(SEM)的标准误差，置信区间为95％。数据表示3次独立重复的平均值±sem。结果显示在图7中。
因此，在不同条件下测量了在35S启动子控制下具有几种形式的AtAHA5的转化植物的气孔孔径，并与野生型(图7A)或ost2-2D(图7B)进行了比较。
在黑暗中，与野生型植物相比，所有植物的孔径相似，但在ost2-2D突变体中受到强烈影响，表明AtAHA5质子泵活性消除了ost2-2D表型。照射3小时后，发现与野生型(图7A)或ost2-2D植物(图7B)相比，AtAHA5过表达系中的气孔孔径大大减少。总之，这些结果表明AtAHA5活性抑制光诱导的气孔开放。确定保卫细胞的大小和气孔密度，并且转基因系和野生型植物之间的两个参数相似(数据未显示)。
实施例10：生物质实验
植物气孔通过打开和关闭来控制碳吸收和蒸腾。因此，当气孔关闭导致气孔孔径减小时，可能会影响气体交换，从而限制碳吸收。这种气体交换的减少可以转化为较低的光合作用速率和植物生物量。为了评估AtAHA5或其组成型活性变体的异位表达对植物生物量的影响，在35S启动子控制下在4周内在生长室中(21℃，70％相对湿度，8小时/16小时光照/黑暗，300μmol.m-2.s-1)的土壤上培育AtAHA5，AtAHA5P50S或AtAHA5W857L转化的拟南芥植物(Col0或ost2-2D)，在光照期开始后至少4小时切下莲叶座并且转移到黑暗中。测量切下的莲座的鲜重。给出平均值和标准偏差(n≥6个植物重复)。
AtAHA5或组成型变体AtAHA5的活性增加不影响标准条件下的植物生物量。过量表达AtAHA5或其组成型变体的植物的生物量不受影响(图8)
实施例11：启动子SbMYB60的表征
将表达pSbMYB60:ZsGreen盒和无效的T1或T2种子分离，用肥料播种在盆中，并置于温室控制的环境中。
通过除草剂抗性试验进行转基因植物的选择。
在每天两次5分钟的正常灌溉下培育每个事件的12个转基因植物，直到第15叶出现。然后每个事件两批6株植物进行两种不同的灌溉处理。如上所述一批得到灌溉，而第二批的灌溉减少到每天两次1分30，直到穗出现然后停止直到开花阶段。一旦植物在开花阶段受到严重胁迫，就进行叶子取样。
为了评估ZsGreen(Clonetech；Matz等，1999)对幼叶缺水的响应，还对玉米幼苗进行了干旱胁迫实验。同时对7个事件中的叶子4进行取样。
A-RT-PCR分析
使用SV96总RNA分离系统(Promega)纯化总RNA。应用DNase处理并控制RNA的完整性。使用MultiScribeTM逆转录酶(Thermo Fisher Scientific)通过逆转录合成cDNA。
通过定量RT-PCR(25℃下10分钟，37℃下2小时，85℃下5分钟)定量ZsGreen转录物。将相对表达标准化为ZmUbiquitin(GRMZM2G110983)和ZmEF1α(GRMZM2G001327)，并根据ΔΔCt方法(Livak等，2001)计算。
如图9所示，在胁迫条件下，幼叶(叶#4)中ZsGreen的表达显著上调约30倍，而事件2则达到50倍。如图10所示，在老叶(叶#10和旗叶)中发现ZsGreen的表达上调约1.5至2倍。除了事件003的叶#10之外，结果是显著的(p值>0.01)。受胁迫的植物的第10叶中ZsGreencDNA的水平在统计学上高于灌溉植物中的水平。该观察与旗叶中的观察相同。
B-共聚焦显微镜观察
按如上所述培育植物，并且当植物受到严重胁迫即进行共聚焦显微镜观察。Adaxial转基因植物叶表皮在显微镜载玻片上被隔绝。将载玻片安装在安装缓冲液(甘油50％，PBS 0,5x)中。使用LSM 800 Airyscan(Carl Zeiss)通过共聚焦显微镜观察气孔，激发波长为488nm，在1个通风单位处针孔为38μm，在494和520nm之间检测到发射。
如图11所示，从受胁迫的植物保卫细胞的观察中检测到的荧光信号大于良好灌溉的植物保卫细胞的荧光信号，显示了ZsGreen在受胁迫的植物中更加上调。
这些结果表明，用于转化的pSbMYB60启动子的片段不仅驱动保卫细胞特异性表达，而且还响应水分亏缺而驱动下游基因的上调。该启动子可被认为是新的干旱诱导型启动子。
参考文献
专利文献
EP2995680
WO2014/012145
EP1033405
US20130074202
US20090217414
US8577624
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Leu Val Ile Asn Ser Thr Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly
115 120 125
Asn Ala Ala Ala Ala Leu Met Ala Asn Leu Ala Pro Lys Thr Lys Val
130 135 140
Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu Ala Ala Ile Leu Val Pro
145 150 155 160
Gly Asp Ile Val Ser Ile Lys Leu Gly Asp Ile Val Pro Ala Asp Ala
165 170 175
Arg Leu Leu Glu Gly Asp Pro Leu Lys Val Asp Gln Ser Ala Leu Thr
180 185 190
Gly Glu Ser Leu Pro Val Thr Lys Gly Pro Gly Asp Glu Val Phe Ser
195 200 205
Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu Ala Val Val Ile Ala Thr
210 215 220
Gly Val His Thr Phe Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr
225 230 235 240
Asn Gln Val Gly His Phe Gln Gln Val Leu Thr Ala Ile Gly Asn Phe
245 250 255
Cys Ile Cys Ser Ile Ala Val Gly Ile Val Val Glu Ile Ile Val Met
260 265 270
Phe Pro Ile Gln His Arg Arg Tyr Arg Ser Gly Ile Glu Asn Leu Leu
275 280 285
Val Leu Leu Ile Gly Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser
290 295 300
Val Thr Met Ala Ile Gly Ser His Lys Leu Ser Gln Gln Gly Ala Ile
305 310 315 320
Thr Lys Arg Met Thr Ala Ile Glu Glu Met Ala Gly Met Asp Val Leu
325 330 335
Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu Asn Lys Leu Ser Val Asp
340 345 350
Lys Asn Leu Val Glu Val Phe Cys Lys Gly Val Asp Lys Asp His Val
355 360 365
Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr Glu Asn Gln Asp Ala Ile
370 375 380
Asp Ala Ala Met Val Gly Met Leu Ala Asp Pro Lys Glu Ala Arg Ala
385 390 395 400
Gly Ile Arg Glu Ile His Phe Leu Pro Phe Asn Pro Val Asp Lys Arg
405 410 415
Thr Ala Leu Thr Tyr Ile Asp Ala Asp Gly His Trp His Arg Val Ser
420 425 430
Lys Gly Ala Pro Glu Gln Ile Leu Asp Leu Cys His Cys Lys Glu Asp
435 440 445
Leu Arg Arg Lys Val His Gly Ile Ile Asp Lys Tyr Ala Glu Arg Gly
450 455 460
Leu Arg Ser Leu Ala Val Ala Arg Gln Glu Val Pro Glu Arg Asn Lys
465 470 475 480
Glu Ser Pro Gly Gly Pro Trp Gln Phe Val Gly Leu Leu Pro Leu Phe
485 490 495
Asp Pro Pro Arg His Asp Ser Ala Glu Thr Ile Arg Lys Ala Leu Val
500 505 510
Leu Gly Val Asn Val Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Gly
515 520 525
Lys Glu Thr Gly Arg Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser
530 535 540
Ser Ala Leu Leu Gly Gln Asn Lys Asp Ala Thr Leu Glu Ala Leu Pro
545 550 555 560
Val Asp Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro
565 570 575
Glu His Lys Tyr Glu Ile Val Lys Arg Leu Gln Glu Lys Lys His Ile
580 585 590
Val Gly Met Thr Gly Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys
595 600 605
Ala Asp Ile Gly Ile Ala Val Ala Asp Ala Thr Asp Ala Ala Arg Ser
610 615 620
Ala Ser Asp Ile Val Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser
625 630 635 640
Ala Val Leu Thr Ser Arg Cys Ile Phe Gln Arg Met Lys Asn Tyr Thr
645 650 655
Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile Val Leu Gly Phe Met Leu
660 665 670
Ile Ala Leu Ile Trp Gln Tyr Asp Phe Ser Pro Phe Met Val Leu Ile
675 680 685
Ile Ala Ile Leu Asn Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg
690 695 700
Val Lys Pro Ser Pro Leu Pro Asp Ser Trp Lys Leu Lys Glu Ile Phe
705 710 715 720
Ala Thr Gly Ile Val Leu Gly Ser Tyr Leu Ala Leu Met Thr Val Ile
725 730 735
Phe Phe Trp Ala Met His Lys Thr Asp Phe Phe Ser Asp Lys Phe Gly
740 745 750
Val Arg Ser Ile Arg Asp Ser Glu His Glu Met Met Ser Ala Leu Tyr
755 760 765
Leu Gln Val Ser Ile Val Ser Gln Ala Leu Ile Phe Val Thr Arg Ser
770 775 780
Arg Ser Trp Ser Phe Val Glu Arg Pro Gly Leu Leu Leu Val Thr Ala
785 790 795 800
Phe Leu Leu Ala Gln Leu Val Ala Thr Phe Leu Ala Val Tyr Ala Asn
805 810 815
Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly Trp Gly Trp Ala Gly Val
820 825 830
Val Trp Leu Tyr Ser Ile Val Phe Tyr Phe Pro Leu Asp Leu Ile Lys
835 840 845
Phe Phe Ile Arg Phe Val Leu Ser Gly Arg Ala Trp Asp Asn Leu Leu
850 855 860
Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr Gly Arg Glu Glu
865 870 875 880
Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg Thr Leu His Gly Leu Gln
885 890 895
Pro Pro Glu Ala Ala Thr Ser Thr Leu Phe His Asp Lys Asn Ser Tyr
900 905 910
Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala Lys Arg Arg Ala Glu Ile
915 920 925
Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys Gly His Val Glu Ser Val
930 935 940
Val Lys Leu Lys Gly Leu Asp Ile Asp Thr Ile Gln Gln Asn Tyr Thr
945 950 955 960
Val
<210> 3
<211> 954
<212> PRT
<213> 向日葵
<220>
<221> misc_feature
<223> HaRA11741_4232_向日葵
<400> 3
Met Gly Thr Asp Lys Ala Leu Gly Leu Glu Glu Ile Lys Asn Glu Thr
1 5 10 15
Val Asp Leu Glu Lys Val Pro Ile Glu Glu Val Phe Gln Gln Leu Lys
20 25 30
Cys Asn Arg Glu Gly Leu Thr Ser Asp Glu Gly Ala Ala Arg Leu Gln
35 40 45
Ile Phe Gly Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu
50 55 60
Lys Phe Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Ala
65 70 75 80
Ala Ala Ile Met Ala Ile Ala Met Ala Asn Gly Gly Gly Lys Pro Pro
85 90 95
Asp Trp Gln Asp Phe Val Gly Ile Val Cys Leu Leu Val Ile Asn Ser
100 105 110
Thr Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala
115 120 125
Leu Met Ala Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg
130 135 140
Trp Ser Glu Gln Glu Ala Ser Ile Leu Val Pro Gly Asp Ile Ile Ser
145 150 155 160
Ile Lys Leu Gly Asp Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly
165 170 175
Asp Pro Leu Lys Ile Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro
180 185 190
Val Asn Lys Asn Pro Tyr Asp Glu Val Phe Ser Gly Ser Thr Cys Lys
195 200 205
Gln Gly Glu Ile Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe
210 215 220
Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr Asn Gln Val Gly His
225 230 235 240
Phe Gln Gln Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile
245 250 255
Ala Val Gly Met Leu Val Glu Ile Ile Val Met Tyr Pro Ile Gln His
260 265 270
Arg Gln Tyr Arg Ser Gly Ile Asp Asn Leu Leu Val Leu Leu Ile Gly
275 280 285
Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile
290 295 300
Gly Ser His Arg Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr
305 310 315 320
Ala Ile Glu Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr
325 330 335
Gly Thr Leu Thr Leu Asn Lys Leu Thr Val Asp Lys Asn Leu Ile Glu
340 345 350
Val Phe Ala Lys Gly Val Asp Lys Glu Gln Val Leu Leu Tyr Ala Ala
355 360 365
Arg Ala Ser Arg Thr Glu Asn Gln Asp Ala Ile Asp Ala Ala Ile Val
370 375 380
Gly Thr Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val
385 390 395 400
His Phe Phe Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr
405 410 415
Ile Asp Glu Arg Gly Asn Trp His Arg Thr Ser Lys Gly Ala Pro Glu
420 425 430
Gln Ile Leu Thr Leu Cys Gly Cys Lys Glu Asp Met Lys Lys Lys Val
435 440 445
His Ala Met Ile Asp Lys Phe Ala Glu Arg Gly Leu Arg Ser Leu Ala
450 455 460
Val Gly Lys Gln Glu Val Pro Glu Lys Thr Lys Glu Gly Pro Gly Gly
465 470 475 480
Pro Trp Gln Phe Val Gly Leu Leu Ser Leu Phe Asp Pro Pro Arg His
485 490 495
Asp Ser Ala Glu Thr Ile Arg Gln Ala Leu His Leu Gly Val Asn Val
500 505 510
Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Ala Lys Glu Thr Gly Arg
515 520 525
Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser Ser Ser Leu Leu Gly
530 535 540
Asp His Lys Asp Ala Ser Ile Ala Thr Leu Pro Ile Glu Glu Leu Ile
545 550 555 560
Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu
565 570 575
Ile Val Lys Lys Leu Gln Glu Arg Lys His Ile Cys Gly Met Thr Gly
580 585 590
Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile
595 600 605
Ala Val Ala Asp Ala Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val
610 615 620
Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser
625 630 635 640
Arg Ala Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser
645 650 655
Ile Thr Ile Arg Ile Val Phe Gly Phe Met Phe Ile Ala Leu Ile Trp
660 665 670
Lys Phe Asp Phe Ser Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn
675 680 685
Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro
690 695 700
Leu Pro Asp Ser Trp Lys Leu Lys Glu Ile Phe Ala Thr Gly Ile Val
705 710 715 720
Leu Gly Gly Tyr Leu Ala Leu Met Thr Val Ile Phe Phe Trp Ile Met
725 730 735
Lys Asp Thr Asp Phe Phe Thr Glu Lys Phe Gly Val Arg Ser Leu Arg
740 745 750
Asn Ser Glu Val Glu Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile
755 760 765
Val Ser Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe
770 775 780
Ile Glu Arg Pro Gly Leu Leu Leu Leu Gly Ala Phe Leu Ala Ala Gln
785 790 795 800
Leu Val Ala Thr Leu Ile Ala Val Tyr Ala Glu Trp Glu Phe Ala Arg
805 810 815
Ile Lys Gly Val Gly Trp Gly Trp Ala Gly Val Ile Trp Leu Tyr Ser
820 825 830
Ile Val Phe Tyr Phe Pro Leu Asp Ile Met Lys Phe Ala Ile Arg Tyr
835 840 845
Ile Leu Ser Gly Lys Ala Trp Arg Asn Met Leu Glu Asn Lys Thr Ala
850 855 860
Phe Thr Thr Lys Lys Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp
865 870 875 880
Ala Leu Ala Gln Arg Thr Leu His Gly Leu Gln Ala Pro Glu Thr Ser
885 890 895
Asn Ile Phe Asn Glu Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala
900 905 910
Glu Gln Ala Lys Arg Arg Ala Glu Val Ala Arg Leu Arg Glu Val Leu
915 920 925
Thr Leu Lys Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp
930 935 940
Ile Asp Thr Ile Gln Gln His Tyr Thr Val
945 950
<210> 4
<211> 952
<212> PRT
<213> 向日葵
<220>
<221> misc_feature
<223> HaRA11742_4232_向日葵
<400> 4
Met Gly Gly Asp Lys Ala Leu Ser Leu Glu Glu Ile Lys Asn Glu Thr
1 5 10 15
Val Asp Leu Glu Lys Val Pro Ile Glu Glu Val Phe Glu Gln Leu Lys
20 25 30
Cys Asn Arg Glu Gly Leu Ser Ser Asp Glu Gly Ala Ala Arg Leu Gln
35 40 45
Ile Phe Gly Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys Leu Leu
50 55 60
Lys Phe Leu Gly Phe Met Asn Pro Leu Ser Trp Val Met Glu Ala Ala
65 70 75 80
Ala Ile Met Ala Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp
85 90 95
Trp Gln Asp Phe Val Gly Ile Val Cys Leu Leu Val Ile Asn Ser Thr
100 105 110
Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu
115 120 125
Met Ala Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Asn Trp
130 135 140
Ser Glu Gln Glu Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile
145 150 155 160
Lys Leu Gly Asp Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp
165 170 175
Pro Leu Lys Ile Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val
180 185 190
Asn Lys Asn Pro Tyr Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln
195 200 205
Gly Ile Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly
210 215 220
Lys Ala Ala His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln
225 230 235 240
Gln Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val
245 250 255
Gly Met Leu Val Glu Ile Val Val Met Tyr Pro Ile Gln His Arg Glu
260 265 270
Tyr Arg Asn Gly Ile Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile
275 280 285
Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser
290 295 300
His Arg Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile
305 310 315 320
Glu Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr
325 330 335
Leu Thr Leu Asn Lys Leu Ser Val Asp Lys Asn Leu Ile Glu Val Phe
340 345 350
Ala Lys Gly Val Asp Lys Asp Gln Val Leu Leu Tyr Ala Ala Arg Ala
355 360 365
Ser Arg Thr Glu Asn Gln Asp Ala Ile Asp Ala Ala Ile Val Gly Thr
370 375 380
Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe
385 390 395 400
Phe Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp
405 410 415
Glu Arg Gly Asn Trp His Arg Thr Ser Lys Gly Ala Pro Glu Gln Ile
420 425 430
Leu Thr Leu Cys Gly Cys Lys Glu Asp Met Lys Lys Lys Val His Ala
435 440 445
Met Ile Asp Lys Phe Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala
450 455 460
Lys Gln Glu Val Pro Glu Lys Asn Lys Glu Ser Pro Gly Gly Pro Trp
465 470 475 480
Thr Phe Ile Gly Leu Leu Ser Leu Phe Asp Pro Pro Arg His Asp Ser
485 490 495
Ala Glu Thr Ile Arg Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met
500 505 510
Ile Thr Gly Asp Gln Leu Ala Ile Ala Lys Glu Thr Gly Arg Arg Leu
515 520 525
Gly Met Gly Thr Asn Met Tyr Pro Ser Ser Ser Leu Leu Gly Asn His
530 535 540
Lys Asp Ala Ser Ile Ala Ala Ile Pro Ile Glu Glu Leu Ile Glu Lys
545 550 555 560
Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val
565 570 575
Lys Lys Leu Gln Glu Arg Lys His Ile Cys Gly Met Thr Gly Asp Gly
580 585 590
Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val
595 600 605
Ala Asp Ala Thr Asp Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr
610 615 620
Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala
625 630 635 640
Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr
645 650 655
Ile Arg Ile Val Phe Gly Phe Leu Phe Ile Ala Leu Ile Trp Lys Phe
660 665 670
Asp Phe Ser Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly
675 680 685
Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro
690 695 700
Asp Ser Trp Lys Leu Lys Glu Ile Phe Ala Thr Gly Ile Val Leu Gly
705 710 715 720
Gly Tyr Leu Ala Leu Met Thr Val Ile Phe Phe Trp Ile Met Lys Asp
725 730 735
Thr Asn Phe Phe Ser Asp Thr Phe Gly Val Arg Ser Leu Arg His Ser
740 745 750
Glu Val Glu Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile Val Ser
755 760 765
Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Ile Glu
770 775 780
Arg Pro Gly Leu Leu Leu Leu Gly Ala Phe Leu Ala Ala Gln Leu Val
785 790 795 800
Ala Thr Leu Ile Ala Val Tyr Ala Asn Trp Glu Phe Ala Arg Ile Lys
805 810 815
Gly Val Gly Trp Gly Trp Ala Gly Val Ile Trp Leu Tyr Ser Ile Val
820 825 830
Phe Tyr Phe Pro Leu Asp Leu Met Lys Phe Ala Ile Arg Tyr Ile Leu
835 840 845
Ser Gly Lys Ala Trp Asn Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr
850 855 860
Ser Lys Lys Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Leu
865 870 875 880
Ala Gln Arg Thr Leu His Gly Leu Gln Gln Pro Glu Thr Ser Asn Ile
885 890 895
Phe Asn Glu Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln
900 905 910
Ala Lys Arg Arg Ala Glu Val Ala Arg Leu Arg Glu Val Leu Thr Leu
915 920 925
Lys Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp
930 935 940
Thr Ile Gln Gln His Tyr Thr Val
945 950
<210> 5
<211> 952
<212> PRT
<213> 西红柿
<220>
<221> misc_feature
<223> LeAHA_XP_004242908.1_西红柿
<400> 5
Met Ala Lys Ala Ile Ser Leu Glu Glu Ile Lys Asn Glu Thr Val Asp
1 5 10 15
Leu Glu Lys Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Ser
20 25 30
Arg Glu Gly Leu Thr Ser Asp Glu Gly Ala Asn Arg Leu Gln Ile Phe
35 40 45
Gly Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu Lys Phe
50 55 60
Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala
65 70 75 80
Ile Met Ala Ile Ala Leu Ala Asn Gly Asp Gly Lys Pro Pro Asp Trp
85 90 95
Gln Asp Phe Val Gly Ile Val Cys Leu Leu Val Ile Asn Ser Thr Ile
100 105 110
Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met
115 120 125
Ala Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Ser
130 135 140
Glu Gln Glu Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Val Lys
145 150 155 160
Leu Gly Asp Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro
165 170 175
Leu Lys Ile Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr
180 185 190
Lys Asn Pro Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly
195 200 205
Glu Leu Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly
210 215 220
Lys Ala Ala His Leu Val Asp Ser Thr Asn Asn Val Gly His Phe Gln
225 230 235 240
Lys Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Ile
245 250 255
Gly Met Leu Val Glu Ile Ile Val Met Tyr Pro Ile Gln His Arg Lys
260 265 270
Tyr Arg Asp Gly Ile Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile
275 280 285
Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser
290 295 300
His Arg Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile
305 310 315 320
Glu Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr
325 330 335
Leu Thr Leu Asn Lys Leu Ser Val Asp Arg Ser Leu Val Glu Val Phe
340 345 350
Thr Lys Gly Val Asp Lys Glu Tyr Val Leu Leu Leu Ala Ala Arg Ala
355 360 365
Ser Arg Val Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met
370 375 380
Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe
385 390 395 400
Leu Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp
405 410 415
Ser Asn Gly Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile
420 425 430
Leu Asp Leu Cys Asn Cys Lys Glu Asp Val Arg Arg Lys Val His Ser
435 440 445
Met Ile Asp Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala
450 455 460
Arg Gln Glu Val Pro Glu Lys Ser Lys Glu Ser Thr Gly Gly Pro Trp
465 470 475 480
Gln Phe Val Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser
485 490 495
Ala Glu Thr Ile Arg Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met
500 505 510
Ile Thr Gly Asp Gln Leu Ala Ile Ala Lys Glu Thr Gly Arg Arg Leu
515 520 525
Gly Met Gly Thr Asn Met Tyr Pro Ser Ala Ser Leu Leu Gly Gln Asp
530 535 540
Lys Asp Ser Ser Ile Ala Ser Leu Pro Val Glu Glu Leu Ile Glu Lys
545 550 555 560
Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val
565 570 575
Lys Lys Leu Gln Glu Arg Lys His Ile Val Gly Met Thr Gly Asp Gly
580 585 590
Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val
595 600 605
Ala Asp Ala Thr Asp Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr
610 615 620
Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala
625 630 635 640
Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr
645 650 655
Ile Arg Ile Val Phe Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Tyr
660 665 670
Asp Phe Ser Ala Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly
675 680 685
Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Met Pro
690 695 700
Asp Ser Trp Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly
705 710 715 720
Gly Tyr Gln Ala Leu Met Thr Val Ile Phe Phe Trp Ala Met His Asp
725 730 735
Thr Ser Phe Phe Thr Asp Lys Phe Gly Val Lys Asp Ile Arg Glu Ser
740 745 750
Asp Glu Glu Met Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Ile Ser
755 760 765
Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu
770 775 780
Arg Pro Gly Ala Leu Leu Met Ile Ala Phe Leu Ile Ala Gln Leu Val
785 790 795 800
Ala Thr Leu Ile Ala Val Tyr Ala Asp Trp Thr Phe Ala Arg Val Lys
805 810 815
Gly Cys Gly Trp Gly Trp Ala Gly Val Ile Trp Ile Phe Ser Ile Val
820 825 830
Thr Tyr Phe Pro Leu Asp Ile Met Lys Phe Ala Ile Arg Tyr Ile Leu
835 840 845
Ser Gly Lys Ala Trp Asn Asn Leu Leu Asp Asn Lys Thr Ala Phe Thr
850 855 860
Thr Lys Lys Asp Tyr Gly Lys Glu Glu Arg Glu Ala Gln Trp Ala Leu
865 870 875 880
Ala Gln Arg Thr Leu His Gly Leu Gln Pro Pro Glu Ala Ser Asn Leu
885 890 895
Phe Asn Glu Lys Asn Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln
900 905 910
Ala Lys Arg Arg Ala Glu Met Ala Arg Leu Arg Glu Leu His Thr Leu
915 920 925
Lys Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Glu
930 935 940
Thr Ile Gln Gln His Tyr Thr Val
945 950
<210> 6
<211> 951
<212> PRT
<213> 獐矛
<220>
<221> misc_feature
<223> AlAHA_G3M3G8_獐矛
<400> 6
Met Gly Gly Leu Glu Glu Ile Lys Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Met Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Ser Ser Glu Glu Gly Thr Gln Arg Leu Gln Val Phe Gly Pro Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Val
165 170 175
Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Gly Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln His Arg Lys Tyr Arg Ser
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Asp His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Ala Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ser Asp Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Arg Glu Asp Met Lys Arg Lys Val His Ser Ile Ile Asp
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Thr Lys Glu Ser Pro Gly Gly Pro Trp Gln Phe Val
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu His Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Asn Lys Asp Ser
530 535 540
Thr Leu Glu Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Lys Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Leu Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Asn Glu Ile Phe Ala Thr Gly Ile Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Ile Met Thr Val Val Phe Phe Trp Ala Ile His Lys Thr Asp Phe
725 730 735
Phe Thr Glu Lys Phe Gly Val Arg Ser Ile Arg Asp Ser Glu His Glu
740 745 750
Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Val Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Val Trp Leu Tyr Ser Val Val Leu Tyr Phe
820 825 830
Pro Leu Asp Val Phe Lys Phe Leu Ile Arg Phe Ala Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Ala Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Pro Pro Glu Val Ala Ser Asn Thr Leu Phe
885 890 895
Asn Glu Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln Asn Tyr Thr Val
945 950
<210> 7
<211> 951
<212> PRT
<213> 獐矛
<220>
<221> misc_feature
<223> AlAHA_G9MC80_獐矛
<400> 7
Met Gly Gly Leu Glu Glu Ile Lys Asn Glu Ser Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Met Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Ser Ser Glu Glu Gly Thr Gln Arg Leu Gln Val Phe Gly Pro Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Val
165 170 175
Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Gly Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly Leu Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln His Arg Arg Tyr Arg Ser
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Asp His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Ala Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ser Asp Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Arg Glu Asp Met Lys Arg Lys Val His Ser Ile Ile Asp
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Thr Lys Glu Ser Pro Gly Gly Pro Trp Gln Phe Val
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu His Leu Gly Val Asn Val Glu Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Asn Lys Asp Ala
530 535 540
Thr Leu Glu Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Lys Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala
595 600 605
Thr Asp Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Leu Leu Ile Ala Leu Ile Trp Lys Phe Asp Leu Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Lys Glu Ile Phe Ala Thr Gly Ile Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Ile Met Thr Val Val Phe Phe Trp Ala Ile His Lys Thr Asp Phe
725 730 735
Phe Thr Glu Lys Phe Gly Val Arg Ser Ile Arg Asp Ser Glu Asp Glu
740 745 750
Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Val Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asp Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Val Trp Leu Tyr Ser Val Val Phe Tyr Leu
820 825 830
Pro Leu Asp Val Phe Lys Phe Leu Ile Arg Phe Ala Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Ala Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Pro Pro Glu Val Ala Ser Asn Thr Leu Phe
885 890 895
Asn Glu Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln Asn Tyr Thr Val
945 950
<210> 8
<211> 951
<212> PRT
<213> 双色高粱
<220>
<221> misc_feature
<223> SbAHA_Sb06g031240_双色高粱
<400> 8
Met Gly Gly Leu Glu Glu Ile Lys Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Ser Ser Ser Glu Gly Gln Gln Arg Leu Glu Ile Phe Gly Pro Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Glu Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Val
165 170 175
Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Arg Gly Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val Gly Ile Ile
245 250 255
Val Glu Ile Ile Val Met Phe Pro Ile Gln His Arg Lys Tyr Arg Ser
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Cys Lys Gly
340 345 350
Val Asp Lys Asp His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Ala Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Gly Asp Gly
405 410 415
Asn Trp His Arg Val Ser Lys Gly Ala Pro Glu Gln Ile Leu Asp Leu
420 425 430
Cys His Cys Lys Glu Asp Leu Arg Arg Lys Val His Gly Ile Ile Asp
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Asn Lys Glu Ser Pro Gly Gly Pro Trp Gln Phe Val
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Asn Lys Asp Ser
530 535 540
Thr Leu Glu Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Tyr Asp Phe Ser
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Lys Glu Ile Phe Ala Thr Gly Ile Val Leu Gly Ser Tyr Leu
705 710 715 720
Ala Ile Met Thr Val Ile Phe Phe Trp Ala Met His Lys Thr Asp Phe
725 730 735
Phe Ser Asp Lys Phe Gly Val Arg Ser Ile Arg Asp Ser Glu His Glu
740 745 750
Met Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Leu Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Phe
785 790 795 800
Leu Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Val Trp Leu Tyr Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Leu Ile Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Pro Pro Glu Ala Ala Thr Asn Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln Asn Tyr Thr Val
945 950
<210> 9
<211> 951
<212> PRT
<213> 小米
<220>
<221> misc_feature
<223> SiAHA_K3Z3J5_小米
<400> 9
Met Gly Gly Leu Glu Glu Ile Lys Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Ser Ser Asn Glu Gly Ala Gln Arg Leu Glu Ile Phe Gly Pro Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Glu Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Val
165 170 175
Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Gly Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Lys Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln His Arg Ala Tyr Arg Ser
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Cys Lys Gly
340 345 350
Val Asp Lys Asp His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Ala Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ser Asp Gly
405 410 415
Asn Trp His Arg Val Ser Lys Gly Ala Pro Glu Gln Ile Leu Asp Leu
420 425 430
Cys Asn Cys Arg Glu Asp Met Arg Arg Lys Val His Ser Ile Ile Asp
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Asp Ala Ser Gly Gly Pro Trp Gln Phe Val
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ser Leu Leu Gly Thr Asn Lys Asp Ser
530 535 540
Thr Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Tyr Asp Phe Ser
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Lys Glu Ile Phe Ala Thr Gly Val Val Leu Gly Ser Tyr Leu
705 710 715 720
Ala Leu Met Thr Val Ile Phe Phe Trp Ala Met His Lys Thr Asp Phe
725 730 735
Phe Pro Glu Lys Phe Gly Val Lys Pro Ile Arg Asp Ser Glu Gly Lys
740 745 750
Met Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Leu Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Phe
785 790 795 800
Leu Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Glu Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Val Trp Leu Tyr Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Leu Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Pro Pro Glu Ala Ala Ser Asn Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln Asn Tyr Thr Val
945 950
<210> 10
<211> 951
<212> PRT
<213> 稻
<220>
<221> misc_feature
<223> Os04g56160_稻
<400> 10
Met Gly Gly Leu Glu Glu Ile Lys Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Ser Ser Glu Glu Gly Asn Arg Arg Ile Glu Met Phe Gly Pro Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Glu Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
165 170 175
Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Thr Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln His Arg Ala Tyr Arg Ser
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Thr Lys Gly
340 345 350
Val Asp Lys Asp His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Ala Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Asp Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Leu Thr Leu
420 425 430
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ala Val Ile Asp
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Glu Ser Ala Gly Gly Pro Trp Gln Phe Val
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu His Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Asn Lys Asp Ala
530 535 540
Ser Leu Glu Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Leu Leu Ile Ala Leu Ile Trp Lys Tyr Asp Phe Ser
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Lys Glu Ile Phe Ala Thr Gly Ile Val Leu Gly Ser Tyr Leu
705 710 715 720
Ala Leu Met Thr Val Ile Phe Phe Trp Ala Met His Lys Thr Asp Phe
725 730 735
Phe Thr Asp Lys Phe Gly Val Arg Ser Ile Arg Asn Ser Glu His Glu
740 745 750
Met Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Ile Glu Arg Pro Gly
770 775 780
Leu Leu Leu Val Thr Ala Phe Met Leu Ala Gln Leu Val Ala Thr Phe
785 790 795 800
Leu Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Ile Trp Leu Tyr Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Glu Asn Lys Ile Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Glu Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Pro Pro Glu Val Ala Ser Asn Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln Asn Tyr Thr Val
945 950
<210> 11
<211> 951
<212> PRT
<213> 二穗短柄草
<220>
<221> misc_feature
<223> BdAHA_Bradi5g24690.1_二穗短柄草
<400> 11
Met Gly Gly Leu Glu Glu Ile Arg Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly
20 25 30
Leu Thr Ser Asp Glu Gly Ala Gln Arg Val Thr Ile Phe Gly Leu Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Glu Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ser Ile Leu Val Pro Gly Asp Ile Val Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
165 170 175
Asp Gln Ser Gly Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Lys Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Val Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg Arg Lys Tyr Arg Ala
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Leu
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Glu His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Val
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Glu Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ser Val Ile Glu
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Asp Ser Pro Gly Gly Pro Trp Gln Phe Ile
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Ser Lys Asp Gly
530 535 540
Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Asp Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Leu Met Thr Val Val Phe Phe Trp Ala Ile His Lys Thr Asp Phe
725 730 735
Phe Thr Asn Lys Phe Gly Val Arg Ser Ile Arg Asn Ser Glu Phe Glu
740 745 750
Leu Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Phe Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asn Trp Glu Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Val Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala Phe Thr Thr Lys Lys
850 855 860
Asp Tyr Gly Arg Gly Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Ala Pro Glu Ser Asn Asn Asn Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Asn Gln Asn Tyr Thr Val
945 950
<210> 12
<211> 951
<212> PRT
<213> 大麦
<220>
<221> misc_feature
<223> HvAHA_M0Z2H5_大麦
<400> 12
Met Gly Gly Leu Glu Glu Ile Arg Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Gln Gly
20 25 30
Leu Thr Ser Asp Glu Gly Ala Gln Arg Val Glu Ile Phe Gly Leu Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ser Ile Leu Val Pro Gly Asp Ile Val Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
165 170 175
Asp Gln Ser Gly Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Ile Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg Arg Lys Tyr Arg Ala
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Leu
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Glu His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Val
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Glu Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ser Val Ile Glu
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Asp Ser Ala Gly Gly Pro Trp Gln Phe Ile
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Ser Lys Asp Gly
530 535 540
Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Asp Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Leu Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Leu Met Thr Val Val Phe Phe Trp Ile Ile His Arg Thr Asp Phe
725 730 735
Phe Thr Asn Lys Phe Gly Val Arg Ser Ile Arg Glu Asn Glu Thr Glu
740 745 750
Lys Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Phe Leu Leu Val Ile Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Ser Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala Phe Thr Thr Lys Glu
850 855 860
Asn Tyr Gly Lys Gly Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Ala Pro Glu Pro Ala Ser His Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Asn Gln Asn Tyr Thr Val
945 950
<210> 13
<211> 956
<212> PRT
<213> 大麦
<220>
<221> misc_feature
<223> HvAHA_Q8H1X2_大麦
<400> 13
Met Ala Asp Lys Glu Ala Gly Asn Leu Glu Ala Val Leu Lys Glu Val
1 5 10 15
Val Asp Leu Glu Asn Ile Pro Leu Glu Glu Val Leu Asp Asn Leu Arg
20 25 30
Cys Ser Arg Glu Gly Leu Thr Ala Glu Gln Ala Gln Gln Arg Leu Gln
35 40 45
Ile Leu Gly Pro Asn Lys Leu Glu Glu Lys Glu Glu Ser Lys Phe Leu
50 55 60
Lys Phe Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Met
65 70 75 80
Ala Ala Ile Met Ala Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro
85 90 95
Asp Trp Gln Asp Phe Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser
100 105 110
Thr Val Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala
115 120 125
Leu Met Ala Asn Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg
130 135 140
Trp Gly Glu Gln Glu Ala Ser Ile Leu Val Pro Gly Thr Leu Ser Ala
145 150 155 160
Ser Ser Leu Val Thr Ser Ser Leu Leu Met Leu Val Cys Leu Glu Gly
165 170 175
Asp Pro Phe Glu Asp Ser Ser Val Trp Ala Tyr Arg Arg Val Ser Pro
180 185 190
Ser Asp Gln Glu Pro Trp Gly Glu Val Phe Ser Gly Ser Thr Cys Lys
195 200 205
Gln Gly Glu Ile Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe
210 215 220
Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr Asn Gln Val Gly His
225 230 235 240
Phe Gln Gln Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Ile Ser Ile
245 250 255
Ala Val Gly Ile Val Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg
260 265 270
Arg Lys Tyr Arg Ala Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly
275 280 285
Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile
290 295 300
Gly Ser His Lys Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr
305 310 315 320
Ala Ile Glu Glu Leu Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr
325 330 335
Gly Thr Leu Thr Leu Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu
340 345 350
Val Phe Ala Lys Gly Val Asp Lys Glu His Val Leu Leu Leu Ala Ala
355 360 365
Arg Ala Ser Arg Val Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val
370 375 380
Gly Met Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val
385 390 395 400
His Phe Leu Pro Phe Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr
405 410 415
Ile Asp Ala Glu Gly Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu
420 425 430
Gln Ile Ile Thr Leu Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val
435 440 445
His Ser Val Ile Glu Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala
450 455 460
Val Ala Arg Gln Glu Val Pro Glu Lys Ser Lys Asp Ser Ala Gly Gly
465 470 475 480
Pro Trp Gln Phe Ile Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His
485 490 495
Asp Ser Ala Glu Thr Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val
500 505 510
Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg
515 520 525
Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly
530 535 540
Gln Ser Lys Asp Gly Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile
545 550 555 560
Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu
565 570 575
Ile Val Lys Arg Leu Gln Glu Lys Lys His Ile Val Gly Met Thr Gly
580 585 590
Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile
595 600 605
Ala Val Asp Asp Ala Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val
610 615 620
Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser
625 630 635 640
Arg Cys Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser
645 650 655
Ile Thr Ile Arg Ile Val Leu Gly Phe Leu Leu Ile Ala Leu Ile Trp
660 665 670
Lys Phe Asp Phe Ala Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn
675 680 685
Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro
690 695 700
Leu Pro Asp Ser Trp Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val
705 710 715 720
Leu Gly Thr Tyr Leu Ala Leu Met Thr Val Val Phe Phe Trp Ile Ile
725 730 735
His Arg Thr Asp Phe Phe Thr Asn Lys Phe Gly Val Arg Ser Ile Arg
740 745 750
Glu Asn Glu Thr Glu Lys Met Ser Ala Leu Tyr Leu Gln Val Ser Ile
755 760 765
Val Ser Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe
770 775 780
Val Glu Arg Pro Gly Phe Leu Leu Val Ile Ala Phe Leu Leu Ala Gln
785 790 795 800
Leu Val Ala Thr Leu Ile Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg
805 810 815
Ile Ser Gly Ile Gly Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser
820 825 830
Ile Val Phe Tyr Phe Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe
835 840 845
Val Leu Ser Gly Arg Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala
850 855 860
Phe Thr Thr Lys Glu Asn Tyr Gly Lys Gly Glu Arg Glu Ala Gln Trp
865 870 875 880
Ala Thr Ala Gln Arg Thr Leu His Gly Leu Gln Ala Pro Glu Pro Ala
885 890 895
Ser His Thr Leu Phe Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu
900 905 910
Ile Ala Glu Gln Ala Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu
915 920 925
Leu Asn Thr Leu Lys Gly His Val Glu Ser Val Val Lys Leu Lys Gly
930 935 940
Leu Asp Ile Asp Thr Ile Asn Gln Asn Tyr Thr Val
945 950 955
<210> 14
<211> 880
<212> PRT
<213> 小麦
<220>
<221> misc_feature
<223> TaAHA_2BL_4AEA2109C_小麦
<400> 14
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
1 5 10 15
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
20 25 30
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
35 40 45
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
50 55 60
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
65 70 75 80
Ala Ser Ile Leu Val Pro Gly Asp Ile Val Ser Ile Lys Leu Gly Asp
85 90 95
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
100 105 110
Asp Gln Ser Gly Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
115 120 125
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
130 135 140
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
145 150 155 160
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
165 170 175
Thr Ala Ile Gly Asn Phe Cys Ile Val Ser Ile Ala Val Gly Ile Val
180 185 190
Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg Arg Lys Tyr Arg Ala
195 200 205
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
210 215 220
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
225 230 235 240
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Leu
245 250 255
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
260 265 270
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
275 280 285
Val Asp Lys Glu His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Val
290 295 300
Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met Leu Ala Asp
305 310 315 320
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
325 330 335
Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Glu Gly
340 345 350
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
355 360 365
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ser Val Ile Glu
370 375 380
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
385 390 395 400
Val Pro Glu Lys Ser Lys Asp Ser Pro Gly Gly Pro Trp Gln Phe Ile
405 410 415
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
420 425 430
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
435 440 445
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
450 455 460
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Ser Lys Asp Gly
465 470 475 480
Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
485 490 495
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
500 505 510
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
515 520 525
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Asp Asp Ala
530 535 540
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
545 550 555 560
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
565 570 575
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
580 585 590
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
595 600 605
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
610 615 620
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
625 630 635 640
Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly Thr Tyr Leu
645 650 655
Ala Leu Met Thr Val Val Phe Phe Trp Val Ile His Lys Thr Asp Phe
660 665 670
Phe Thr Asn Lys Phe Gly Val Arg Ser Ile Arg Asp Ser Glu Phe Glu
675 680 685
Met Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
690 695 700
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
705 710 715 720
Phe Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
725 730 735
Ile Ala Val Tyr Ala Asn Trp Glu Phe Ala Arg Ile Lys Gly Ile Gly
740 745 750
Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser Ile Val Phe Tyr Phe
755 760 765
Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
770 775 780
Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala Phe Thr Thr Lys Glu
785 790 795 800
Asn Tyr Gly Lys Gly Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
805 810 815
Thr Leu His Gly Leu Gln Ala Pro Glu Pro Ala Ser His Thr Leu Phe
820 825 830
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
835 840 845
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
850 855 860
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
865 870 875 880
<210> 15
<211> 951
<212> PRT
<213> 小麦
<220>
<221> misc_feature
<223> TaAHA_2AL_2C50DEE4C.1_小麦
<400> 15
Met Gly Gly Leu Glu Glu Ile Arg Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Gln Gly
20 25 30
Leu Thr Ser Asp Glu Gly Ala Gln Arg Val Glu Ile Phe Gly Leu Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu
130 135 140
Ala Ser Ile Leu Val Pro Gly Asp Ile Val Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
165 170 175
Asp Gln Ser Gly Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Val Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg Arg Lys Tyr Arg Ala
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Leu
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Glu His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Val
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Glu Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ser Val Ile Glu
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Asp Ser Pro Gly Gly Pro Trp Gln Phe Ile
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Ser Lys Asp Gly
530 535 540
Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Asp Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Leu Val Thr Val Val Phe Phe Trp Leu Ile His Lys Thr Asp Phe
725 730 735
Phe Thr Asn Lys Phe Gly Val Glu Ser Ile Arg Asn Thr Glu Phe Lys
740 745 750
Glu Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Phe Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asn Trp Asp Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala Phe Thr Thr Lys Glu
850 855 860
Asn Tyr Gly Lys Gly Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Ala Pro Glu Pro Ala Ser His Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Asn Gln Asn Tyr Thr Val
945 950
<210> 16
<211> 951
<212> PRT
<213> 小麦
<220>
<221> misc_feature
<223> TaAHA_Q5PSM6_小麦
<400> 16
Met Gly Gly Leu Glu Glu Ile Arg Asn Glu Ala Val Asp Leu Glu Asn
1 5 10 15
Ile Pro Ile Glu Glu Val Phe Glu Gln Leu Lys Cys Thr Arg Gln Gly
20 25 30
Leu Thr Ser Asp Glu Gly Ala Gln Arg Val Glu Ile Phe Gly Leu Asn
35 40 45
Lys Leu Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe
50 55 60
Met Trp Asn Pro Leu Ser Trp Val Met Glu Met Ala Ala Ile Met Ala
65 70 75 80
Ile Ala Leu Ala Asn Gly Gly Gly Lys Pro Pro Asp Trp Gln Asp Phe
85 90 95
Val Gly Ile Ile Val Leu Leu Val Ile Asn Ser Thr Ile Ser Phe Ile
100 105 110
Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Asn Leu
115 120 125
Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg Trp Gly Glu Gln Gly
130 135 140
Ala Ser Ile Leu Val Pro Gly Asp Ile Val Ser Ile Lys Leu Gly Asp
145 150 155 160
Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu Lys Ile
165 170 175
Asp Gln Ser Gly Leu Thr Gly Glu Ser Leu Pro Val Thr Lys Asn Pro
180 185 190
Gly Asp Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu
195 200 205
Ala Val Val Ile Ala Thr Gly Val Arg Thr Phe Phe Gly Lys Ala Ala
210 215 220
His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Gln Val Leu
225 230 235 240
Thr Ala Ile Gly Asn Phe Cys Ile Val Ser Ile Ala Val Gly Ile Val
245 250 255
Ile Glu Ile Ile Val Met Phe Pro Ile Gln Arg Arg Lys Tyr Arg Ala
260 265 270
Gly Ile Glu Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala
275 280 285
Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Lys Leu
290 295 300
Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Leu
305 310 315 320
Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu
325 330 335
Asn Lys Leu Ser Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly
340 345 350
Val Asp Lys Glu His Val Leu Leu Leu Ala Ala Arg Ala Ser Arg Val
355 360 365
Glu Asn Gln Asp Ala Ile Asp Ala Cys Met Val Gly Met Leu Ala Asp
370 375 380
Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu Pro Phe
385 390 395 400
Asn Pro Thr Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ala Glu Gly
405 410 415
Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile Thr Leu
420 425 430
Cys Asn Cys Lys Glu Asp Val Lys Arg Lys Val His Ser Val Ile Glu
435 440 445
Lys Tyr Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu
450 455 460
Val Pro Glu Lys Ser Lys Asp Ser Pro Gly Gly Pro Trp Gln Phe Ile
465 470 475 480
Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr
485 490 495
Ile Arg Lys Ala Leu Val Leu Gly Val Asn Val Lys Met Ile Thr Gly
500 505 510
Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly
515 520 525
Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Ser Lys Asp Gly
530 535 540
Ser Leu Glu Ser Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly
545 550 555 560
Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys Arg Leu
565 570 575
Gln Glu Lys Lys His Ile Val Gly Met Thr Gly Asp Gly Val Asn Asp
580 585 590
Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Asp Asp Ala
595 600 605
Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu Pro Gly
610 615 620
Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Cys Ile Phe Gln
625 630 635 640
Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile
645 650 655
Val Leu Gly Phe Met Leu Ile Ala Leu Ile Trp Lys Phe Asp Phe Ala
660 665 670
Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met
675 680 685
Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp Ser Trp
690 695 700
Lys Leu Asn Glu Ile Phe Ala Thr Gly Val Val Leu Gly Thr Tyr Leu
705 710 715 720
Ala Leu Val Thr Val Val Phe Phe Trp Leu Ile His Lys Thr Asp Phe
725 730 735
Phe Thr Asn Lys Phe Gly Val Glu Ser Ile Arg Asn Thr Glu Phe Lys
740 745 750
Glu Met Ser Ala Leu Tyr Leu Gln Val Ser Ile Val Ser Gln Ala Leu
755 760 765
Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly
770 775 780
Phe Leu Leu Val Thr Ala Phe Leu Leu Ala Gln Leu Val Ala Thr Leu
785 790 795 800
Ile Ala Val Tyr Ala Asn Trp Asp Phe Ala Arg Ile Lys Gly Ile Gly
805 810 815
Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser Ile Val Phe Tyr Phe
820 825 830
Pro Leu Asp Ile Phe Lys Phe Phe Ile Arg Phe Val Leu Ser Gly Arg
835 840 845
Ala Trp Asp Asn Leu Leu Gln Asn Lys Thr Ala Phe Thr Thr Lys Glu
850 855 860
Asn Tyr Gly Lys Gly Glu Arg Glu Ala Gln Trp Ala Thr Ala Gln Arg
865 870 875 880
Thr Leu His Gly Leu Gln Ala Pro Glu Pro Ala Ser His Thr Leu Phe
885 890 895
Asn Asp Lys Ser Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Ile Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys
915 920 925
Ser His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Asn Gln Asn Tyr Thr Val
945 950
<210> 17
<211> 931
<212> PRT
<213> 荠菜
<220>
<221> misc_feature
<223> CrAHA|XP_006293643_荠菜
<400> 17
Met Glu Glu Val Phe Glu Glu Leu Lys Cys Thr Lys Gln Gly Leu Thr
1 5 10 15
Arg Glu Glu Ala Ser His Arg Leu Asp Leu Phe Gly Pro Asn Lys Leu
20 25 30
Glu Glu Lys Lys Glu Ser Lys Val Leu Lys Phe Leu Gly Phe Met Trp
35 40 45
Asn Pro Leu Ser Trp Val Met Glu Ala Ala Ala Leu Met Ala Ile Ala
50 55 60
Leu Ala Asn Gly Gly Gly Arg Pro Pro Asp Trp Gln Asp Phe Val Gly
65 70 75 80
Ile Val Cys Leu Leu Phe Ile Asn Ser Thr Ile Ser Phe Ile Glu Glu
85 90 95
Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Gly Leu Ala Pro
100 105 110
Lys Thr Lys Val Leu Arg Asp Asn Gln Trp Ser Glu Gln Glu Ala Ser
115 120 125
Ile Leu Val Pro Gly Asp Val Ile Ser Val Lys Leu Gly Asp Ile Ile
130 135 140
Pro Ala Asp Ala Arg Leu Leu Asp Gly Asp Pro Leu Lys Ile Asp Gln
145 150 155 160
Ser Ser Leu Thr Gly Glu Ser Ile Pro Val Thr Lys Asn Pro Gly Asp
165 170 175
Glu Val Phe Ser Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu Ala Ile
180 185 190
Val Val Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala His Leu
195 200 205
Val Asp Ser Thr Asn Gln Ile Gly His Phe Gln Lys Val Leu Thr Ser
210 215 220
Ile Gly Asn Phe Cys Ile Cys Ser Ile Gly Leu Gly Ile Ile Val Glu
225 230 235 240
Leu Ile Val Met Tyr Pro Ile Gln Arg Arg Lys Tyr Arg Asp Gly Ile
245 250 255
Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala Met Pro
260 265 270
Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His Arg Leu Ser Lys
275 280 285
Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met Ala Gly
290 295 300
Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu Asn Arg
305 310 315 320
Leu Thr Val Asp Arg Asn Leu Val Glu Val Phe Ala Lys Gly Val Gly
325 330 335
Lys Glu His Val Phe Leu Leu Ala Ala Arg Ala Ser Arg Ile Glu Asn
340 345 350
Gln Asp Ala Ile Asp Ala Ala Ile Val Gly Met Leu Gly Asp Pro Lys
355 360 365
Glu Ala Arg Ala Gly Val Arg Glu Val His Phe Phe Pro Phe Asn Pro
370 375 380
Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ser Asp Gly Asn Trp
385 390 395 400
His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Leu Asn Leu Cys Asn
405 410 415
Cys Lys Glu Asp Val Arg Arg Lys Val His Gly Val Ile Asp Lys Phe
420 425 430
Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Lys Val Pro
435 440 445
Glu Lys Lys Lys Asp Ala Ser Gly Asp Pro Trp Gln Leu Val Gly Leu
450 455 460
Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr Ile Arg
465 470 475 480
Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met Ile Thr Gly Asp Gln
485 490 495
Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly Thr Asn
500 505 510
Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Glu Lys Asp Ser Ser Leu
515 520 525
Gly Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala
530 535 540
Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Asn Arg Leu Gln Gln
545 550 555 560
Arg Lys His Ile Cys Gly Met Thr Gly Asp Gly Val Asn Asp Ala Pro
565 570 575
Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala Asp Ala Thr Asp
580 585 590
Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr Glu Pro Gly Leu Ser
595 600 605
Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala Ile Phe Gln Arg Met
610 615 620
Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile Val Phe
625 630 635 640
Gly Phe Met Phe Ile Ala Leu Ile Trp Gln Phe Asp Phe Ser Pro Phe
645 650 655
Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met Thr Ile
660 665 670
Ser Lys Asp Arg Val Lys Pro Ser Pro Gln Pro Asp Ser Trp Lys Leu
675 680 685
Arg Glu Ile Phe Ser Thr Gly Val Val Leu Gly Gly Tyr Gln Ala Leu
690 695 700
Met Thr Val Val Phe Phe Trp Val Met Lys Asp Thr Asp Ile Phe Ser
705 710 715 720
Asn Met Leu Gly Val Arg Pro Leu Ser Gln Arg Pro Glu Gln Met Met
725 730 735
Ala Ala Leu Tyr Leu Gln Val Ser Ile Ile Ser Gln Ala Leu Ile Phe
740 745 750
Val Thr Arg Ser Arg Ser Trp Ser Phe Val Glu Arg Pro Gly Leu Leu
755 760 765
Leu Leu Gly Ala Phe Val Ile Ala Gln Leu Val Ala Thr Phe Ile Ala
770 775 780
Val Tyr Ala Asn Trp Ser Phe Ala Arg Ile Glu Gly Ala Gly Trp Gly
785 790 795 800
Trp Ala Gly Val Ile Trp Leu Tyr Ser Leu Ile Thr Tyr Ile Pro Leu
805 810 815
Asp Leu Leu Lys Phe Gly Ile Arg Tyr Val Leu Ser Gly Lys Ala Trp
820 825 830
Leu Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr
835 840 845
Gly Lys Glu Glu Arg Glu Ala Gln Trp Ala Ala Ala Gln Arg Thr Leu
850 855 860
His Gly Leu Gln Pro Ala Glu Thr Asn Asn Ile Phe Asn Glu Lys Asn
865 870 875 880
Ser Tyr His Asp Leu Ser Gln Ile Ala Glu Gln Ala Lys Arg Arg Ala
885 890 895
Glu Val Val Arg Leu Arg Glu Ile Asn Thr Leu Lys Gly His Val Glu
900 905 910
Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr Ile Gln Gln His
915 920 925
Tyr Thr Val
930
<210> 18
<211> 957
<212> PRT
<213> 荠蓝
<220>
<221> misc_feature
<223> CsAHA|XP_010472461_荠蓝
<400> 18
Met Ala Ala Ala Ala Gly Lys Gly Asn Glu Leu Asp His Ile Lys Asn
1 5 10 15
Glu Ser Val Asp Leu Val Arg Ile Pro Met Glu Glu Val Phe Glu Glu
20 25 30
Leu Lys Cys Thr Lys Gln Gly Leu Thr Thr Asp Glu Ala Ser His Arg
35 40 45
Leu Asp Leu Phe Gly Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys
50 55 60
Leu Leu Lys Phe Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met
65 70 75 80
Glu Ala Ala Ala Leu Met Ala Ile Ala Leu Ala Asn Gly Gly Gly Arg
85 90 95
Pro Pro Asp Trp Gln Asp Phe Val Gly Ile Val Cys Leu Leu Phe Ile
100 105 110
Asn Ser Thr Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala
115 120 125
Ala Ala Leu Met Ala Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp
130 135 140
Asn Gln Trp Ser Glu Gln Glu Ala Ser Ile Leu Val Pro Gly Asp Val
145 150 155 160
Ile Ser Leu Lys Leu Gly Asp Ile Ile Pro Ala Asp Ala Arg Leu Leu
165 170 175
Asp Gly Asp Pro Leu Lys Ile Asp Gln Ser Ser Leu Thr Gly Glu Ser
180 185 190
Ile Pro Val Thr Lys Asn Pro Gly Asp Glu Val Phe Ser Gly Ser Thr
195 200 205
Cys Lys Gln Gly Glu Ile Glu Ala Ile Val Val Ala Thr Gly Val His
210 215 220
Thr Phe Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr Asn Gln Ile
225 230 235 240
Gly His Phe Gln Lys Val Leu Thr Ser Ile Gly Asn Phe Cys Ile Cys
245 250 255
Ser Ile Ala Leu Gly Ile Val Val Glu Leu Leu Val Met Tyr Pro Ile
260 265 270
Gln Arg Arg Gly Tyr Arg Asp Gly Ile Asp Asn Leu Leu Val Leu Leu
275 280 285
Ile Gly Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met
290 295 300
Ala Ile Gly Ser His Arg Leu Ser Lys Gln Gly Ala Ile Thr Lys Arg
305 310 315 320
Met Thr Ala Ile Glu Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp
325 330 335
Lys Thr Gly Thr Leu Thr Leu Asn Lys Leu Thr Val Asp Arg Asn Leu
340 345 350
Val Glu Val Phe Ala Lys Gly Val Gly Lys Glu His Val Phe Leu Leu
355 360 365
Ala Ala Arg Ala Ser Arg Ile Glu Asn Gln Asp Ala Ile Asp Ala Ala
370 375 380
Ile Val Gly Met Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Val Arg
385 390 395 400
Glu Val His Phe Phe Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu
405 410 415
Thr Tyr Val Asp Tyr Ser Asp Gly Asn Trp Tyr Arg Ala Ser Lys Gly
420 425 430
Ala Pro Glu Gln Ile Leu Asn Leu Cys Asp Cys Lys Glu Asp Val Arg
435 440 445
Arg Lys Val His Gly Val Ile Asp Lys Phe Ala Glu Arg Gly Leu Arg
450 455 460
Ser Leu Ala Val Ala Arg Gln Glu Val Pro Glu Lys Lys Lys Asp Ala
465 470 475 480
Ser Gly Gly Pro Trp Gln Leu Val Gly Leu Leu Pro Leu Phe Asp Pro
485 490 495
Pro Arg His Asp Ser Ala Glu Thr Ile Arg Arg Ala Leu Asn Leu Gly
500 505 510
Val Asn Val Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Gly Lys Glu
515 520 525
Thr Gly Arg Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser Ser Ala
530 535 540
Leu Leu Gly Gln Glu Lys Asp Ser Ser Leu Gly Ala Leu Pro Val Asp
545 550 555 560
Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His
565 570 575
Lys Tyr Glu Ile Val Asn Arg Leu Gln Gln Arg Lys His Ile Cys Gly
580 585 590
Met Thr Gly Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp
595 600 605
Ile Gly Ile Ala Val Ala Asp Ser Thr Asp Ala Ala Arg Gly Ala Ser
610 615 620
Asp Ile Val Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val
625 630 635 640
Leu Thr Ser Arg Ala Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr
645 650 655
Ala Val Ser Ile Thr Ile Arg Ile Val Phe Gly Phe Met Phe Ile Ala
660 665 670
Leu Ile Trp Gln Phe Asp Phe Ser Pro Phe Met Val Leu Ile Ile Ala
675 680 685
Ile Leu Asn Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys
690 695 700
Pro Ser Pro Gln Pro Asp Ser Trp Lys Leu Arg Glu Ile Phe Ser Thr
705 710 715 720
Gly Val Val Leu Gly Gly Tyr Gln Ala Phe Met Thr Val Val Phe Phe
725 730 735
Trp Val Met Lys Asp Thr Asn Ile Phe Ser Asn Met Leu Gly Val Arg
740 745 750
Pro Leu Ser Gln Arg Pro Glu Gln Met Met Ala Ala Leu Tyr Leu Gln
755 760 765
Val Ser Ile Ile Ser Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser
770 775 780
Trp Ser Phe Val Glu Arg Pro Gly Leu Leu Leu Leu Gly Ala Phe Val
785 790 795 800
Ile Ala Gln Leu Val Ala Thr Phe Ile Ala Val Tyr Ala Asp Trp Ser
805 810 815
Phe Ala Arg Ile Glu Gly Ala Gly Trp Gly Trp Ala Gly Val Ile Trp
820 825 830
Leu Tyr Ser Leu Ile Thr Tyr Ile Pro Leu Asp Leu Leu Lys Phe Gly
835 840 845
Ile Arg Tyr Val Leu Ser Gly Lys Ala Trp Leu Asn Leu Leu Glu Asn
850 855 860
Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr Gly Lys Glu Glu Arg Glu
865 870 875 880
Ala Gln Trp Ala Ala Ala Gln Arg Thr Leu His Gly Leu Gln Pro Ala
885 890 895
Glu Thr Asn Asn Ile Phe Asn Glu Lys Asn Ser Tyr Ser Glu Leu Ser
900 905 910
Gln Ile Ala Glu Gln Ala Lys Arg Arg Ala Glu Val Val Arg Leu Arg
915 920 925
Glu Ile Asn Ser Leu Lys Gly His Val Glu Ser Val Val Lys Leu Lys
930 935 940
Gly Leu Asp Ile Asp Thr Ile Gln Gln His Tyr Thr Val
945 950 955
<210> 19
<211> 951
<212> PRT
<213> 蒺藜苜蓿
<220>
<221> misc_feature
<223> MtAHA|XP_013465721_蒺藜苜蓿
<400> 19
Met Gly Gly Ile Ser Leu Glu Glu Ile Lys Asn Glu Ser Val Asp Leu
1 5 10 15
Glu Arg Ile Pro Ile Asp Glu Val Phe Glu Gln Leu Lys Cys Ser Arg
20 25 30
Gln Gly Leu Thr Ser Glu Glu Gly Ala Asn Arg Leu Gln Val Phe Gly
35 40 45
Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys Phe Leu Lys Phe Leu
50 55 60
Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Ala Ala Ala Ile
65 70 75 80
Met Ala Ile Ala Leu Ala Asn Gly Ser Gly Arg Pro Pro Asp Trp Gln
85 90 95
Asp Phe Val Gly Ile Ile Ala Leu Leu Val Ile Asn Ser Thr Ile Ser
100 105 110
Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala
115 120 125
Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Ser Arg Trp Ser Glu
130 135 140
Gln Asp Ala Ala Ile Leu Val Pro Gly Asp Ile Ile Ser Ile Lys Leu
145 150 155 160
Gly Asp Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly Asp Pro Leu
165 170 175
Ser Ile Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro Val Thr Lys
180 185 190
Ser Ser Ser Asp Glu Val Phe Ser Gly Ser Thr Val Lys Lys Gly Glu
195 200 205
Ile Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys
210 215 220
Ala Ala His Leu Val Asp Ser Thr Asn Gln Val Gly His Phe Gln Lys
225 230 235 240
Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Ile Gly
245 250 255
Ile Leu Val Glu Leu Ile Val Met Tyr Pro Ile Gln His Arg Lys Tyr
260 265 270
Arg Asp Gly Ile Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro
275 280 285
Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile Gly Ser His
290 295 300
Arg Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu
305 310 315 320
Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr Gly Thr Leu
325 330 335
Thr Leu Asn Lys Leu Ser Val Asp Arg Asn Leu Ile Glu Val Phe Ala
340 345 350
Lys Gly Ile Glu Lys Glu Tyr Val Met Leu Leu Ala Ala Arg Ala Ser
355 360 365
Arg Thr Glu Asn Gln Asp Ala Ile Asp Ala Ala Ile Val Gly Met Leu
370 375 380
Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val His Phe Leu
385 390 395 400
Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr Ile Asp Ser
405 410 415
Asp Gly Lys Trp His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Ile
420 425 430
Asn Leu Cys Asn Cys Lys Glu Asp Val Arg Lys Lys Val His Ala Val
435 440 445
Ile Asp Lys Phe Ala Glu Arg Gly Leu Arg Ser Leu Gly Val Ala Arg
450 455 460
Gln Glu Val Pro Glu Arg Thr Lys Asp Ser Pro Gly Gly Pro Trp Gln
465 470 475 480
Phe Val Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala
485 490 495
Glu Thr Ile Arg Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met Ile
500 505 510
Thr Gly Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly
515 520 525
Met Gly Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Asp Lys
530 535 540
Asp Ser Ser Ile Ser Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala
545 550 555 560
Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val Lys
565 570 575
Arg Leu Gln Glu Lys Lys His Ile Cys Gly Met Thr Gly Asp Gly Val
580 585 590
Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Ala
595 600 605
Asp Ala Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val Leu Thr Glu
610 615 620
Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala Ile
625 630 635 640
Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile
645 650 655
Arg Ile Val Phe Gly Phe Met Phe Ile Ala Leu Ile Trp Lys Phe Asp
660 665 670
Phe Ala Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr
675 680 685
Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro Leu Pro Asp
690 695 700
Ser Trp Lys Leu Arg Glu Ile Phe Ala Thr Gly Val Val Leu Gly Ser
705 710 715 720
Tyr Met Ala Leu Met Thr Val Val Phe Phe Trp Ala Met Lys Asp Thr
725 730 735
Asn Phe Phe Ser Asn Lys Phe Gly Val Arg Pro Ile Arg His Asn Pro
740 745 750
Asp Glu Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile Ile Ser Gln
755 760 765
Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe Ala Glu Arg
770 775 780
Pro Gly Leu Leu Leu Leu Gly Ala Phe Leu Ile Ala Gln Leu Ile Ala
785 790 795 800
Thr Phe Ile Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg Ile Lys Gly
805 810 815
Met Gly Trp Gly Trp Ala Gly Val Ile Trp Leu Tyr Ser Leu Val Thr
820 825 830
Tyr Ile Pro Leu Asp Leu Leu Lys Phe Ala Ile Arg Tyr Phe Leu Ser
835 840 845
Gly Lys Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr
850 855 860
Lys Lys Asp Tyr Gly Lys Glu Glu Arg Glu Ala Gln Trp Ala Ala Ala
865 870 875 880
Gln Arg Thr Leu His Gly Leu Gln Pro Pro Glu Ala Ser Asn Val Phe
885 890 895
Asn Glu Lys Asn Ser Tyr Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala
900 905 910
Lys Arg Arg Ala Glu Val Ala Arg Leu Arg Glu Val His Thr Leu Lys
915 920 925
Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr
930 935 940
Ile Gln Gln His Tyr Thr Val
945 950
<210> 20
<211> 955
<212> PRT
<213> 胡杨
<220>
<221> misc_feature
<223> PeAHA|XP_011031360_胡杨
<400> 20
Met Ser Ser Lys Gly Gly Ile Ser Leu Glu Glu Ile Lys Asn Glu Ser
1 5 10 15
Val Asp Leu Glu Arg Ile Pro Met Glu Glu Val Phe Glu Gln Leu Lys
20 25 30
Cys Thr Arg Glu Gly Leu Ser Ala Asp Glu Gly Ala Ser Arg Leu Gln
35 40 45
Val Phe Gly Pro Asn Lys Leu Glu Glu Lys Lys Glu Ser Lys Ile Leu
50 55 60
Lys Phe Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Ala
65 70 75 80
Ala Ala Leu Met Ala Ile Ala Leu Ala Asn Gly Asp Gly Arg Pro Pro
85 90 95
Asp Trp Gln Asp Phe Val Gly Ile Val Val Leu Leu Val Ile Asn Ser
100 105 110
Thr Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly Asn Ala Ala Ala Ala
115 120 125
Leu Met Ala Gly Leu Ala Pro Lys Thr Lys Val Leu Arg Asp Gly Arg
130 135 140
Trp Ser Glu Gln Asp Ala Ser Ile Leu Val Pro Gly Asp Ile Ile Ser
145 150 155 160
Ile Lys Leu Gly Asp Ile Val Pro Ala Asp Ala Arg Leu Leu Glu Gly
165 170 175
Asp Pro Leu Lys Ile Asp Gln Ser Ala Leu Thr Gly Glu Ser Leu Pro
180 185 190
Val Thr Lys Asn Pro Ser Asp Glu Val Phe Ser Gly Ser Thr Cys Lys
195 200 205
Gln Gly Glu Ile Glu Ala Val Val Ile Ala Thr Gly Val His Thr Phe
210 215 220
Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr Asn Gln Val Gly His
225 230 235 240
Phe Gln Lys Val Leu Thr Ala Ile Gly Asn Phe Cys Ile Cys Ser Ile
245 250 255
Ala Val Gly Ile Phe Ala Glu Leu Ile Val Met Tyr Pro Ile Gln His
260 265 270
Arg Lys Tyr Arg Asp Gly Ile Asp Asn Leu Leu Val Leu Leu Ile Gly
275 280 285
Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser Val Thr Met Ala Ile
290 295 300
Gly Ser His Arg Leu Ser Gln Gln Gly Ala Ile Thr Lys Arg Met Thr
305 310 315 320
Ala Ile Glu Glu Met Ala Gly Met Asp Val Leu Cys Ser Asp Lys Thr
325 330 335
Gly Thr Leu Thr Leu Asn Lys Leu Thr Val Asp Arg Ser Leu Ile Glu
340 345 350
Val Phe Ala Lys Gly Val Glu Lys Glu His Val Ile Leu Leu Ala Ala
355 360 365
Arg Ala Ser Arg Thr Glu Asn Gln Asp Ala Ile Asp Ala Ala Ile Val
370 375 380
Gly Met Leu Ala Asp Pro Lys Glu Ala Arg Ala Gly Ile Arg Glu Val
385 390 395 400
His Phe Leu Pro Phe Asn Pro Val Asp Lys Arg Thr Ala Leu Thr Tyr
405 410 415
Ile Asp Ser Asp Gly Asn Trp His Arg Ala Ser Lys Gly Ala Pro Glu
420 425 430
Gln Ile Leu Thr Leu Cys Asn Cys Lys Glu Asp Val Lys Lys Lys Val
435 440 445
His Ser Val Ile Asp Lys Phe Ala Glu Arg Gly Leu Arg Ser Leu Gly
450 455 460
Val Ala Lys Gln Glu Val Pro Glu Lys Ser Lys Asp Ala Ala Gly Ala
465 470 475 480
Pro Trp Gln Leu Val Gly Leu Leu Pro Leu Phe Asp Pro Pro Arg His
485 490 495
Asp Ser Ala Glu Thr Ile Arg Arg Ala Leu His Leu Gly Val Asn Val
500 505 510
Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Gly Lys Glu Thr Gly Arg
515 520 525
Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser Ser Ala Leu Leu Gly
530 535 540
Gln Asp Arg Asp Ala Ser Ile Ala Ala Leu Pro Val Asp Glu Leu Ile
545 550 555 560
Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro Glu His Lys Tyr Glu
565 570 575
Ile Val Lys Arg Leu Gln Asp Arg Lys His Ile Cys Gly Met Thr Gly
580 585 590
Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile
595 600 605
Ala Val Ala Asp Ala Thr Asp Ala Ala Arg Ser Ala Ser Asp Ile Val
610 615 620
Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser Ala Val Leu Thr Ser
625 630 635 640
Arg Ala Ile Phe Gln Arg Met Lys Asn Tyr Thr Ile Tyr Ala Val Ser
645 650 655
Ile Thr Ile Arg Ile Val Phe Gly Phe Met Phe Ile Ala Leu Ile Trp
660 665 670
Lys Phe Asp Phe Ala Pro Phe Met Val Leu Ile Ile Ala Ile Leu Asn
675 680 685
Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg Val Lys Pro Ser Pro
690 695 700
Gln Pro Asp Ser Trp Lys Leu Lys Glu Ile Phe Ser Thr Gly Ile Val
705 710 715 720
Leu Gly Gly Tyr Met Ala Leu Met Thr Val Leu Phe Phe Trp Ile Met
725 730 735
Lys Asp Thr Asp Phe Phe Ser Asp Lys Phe Gly Val Arg Ser Leu Arg
740 745 750
Lys Asn Asp Glu Glu Met Met Ala Ala Leu Tyr Leu Gln Val Ser Ile
755 760 765
Val Ser Gln Ala Leu Ile Phe Val Thr Arg Ser Arg Ser Trp Ser Phe
770 775 780
Val Glu Arg Pro Gly Phe Leu Leu Leu Gly Ala Phe Val Ala Ala Gln
785 790 795 800
Leu Ile Ala Thr Leu Ile Ala Val Tyr Ala Asn Trp Gly Phe Ala Arg
805 810 815
Ile Lys Gly Cys Gly Trp Gly Trp Ala Gly Val Ile Trp Leu Phe Ser
820 825 830
Val Val Thr Tyr Val Pro Leu Asp Ile Leu Lys Phe Ala Ile Arg Tyr
835 840 845
Ile Leu Ser Gly Lys Ala Trp Asp Asn Leu Leu Glu Asn Lys Thr Ala
850 855 860
Phe Thr Thr Lys Lys Asp Tyr Gly Lys Glu Glu Arg Glu Ala Gln Trp
865 870 875 880
Ala Thr Ala Gln Arg Thr Leu His Gly Leu Gln Pro Pro Glu Thr Ser
885 890 895
His Asn Met Phe Ser Glu Lys Asn Ser Tyr Arg Glu Leu Ser Glu Ile
900 905 910
Ala Glu Gln Ala Lys Arg Arg Ala Glu Met Ala Arg Leu Arg Glu Leu
915 920 925
Asn Thr Leu Lys Gly His Val Glu Ser Val Val Lys Leu Lys Gly Leu
930 935 940
Asp Ile Asp Thr Ile Gln Gln His Tyr Thr Val
945 950 955
<210> 21
<211> 23
<212> PRT
<213> 人工的
<220>
<223> 共有序列第一跨膜结构域
<220>
<221> VARIANT
<222> (15)..(15)
<223> Xaa可以替换为M, V, A
<220>
<221> VARIANT
<222> (18)..(18)
<223> Xaa可以替换为L, I, V
<220>
<221> VARIANT
<222> (23)..(23)
<223> Xaa可以替换为L, M
<400> 21
Phe Leu Gly Phe Met Trp Asn Pro Leu Ser Trp Val Met Glu Xaa Ala
1 5 10 15
Ala Xaa Met Ala Ile Ala Xaa
20
<210> 22
<211> 23
<212> PRT
<213> 人工的
<220>
<223> 第一跨膜结构域在第8位突变 P->S
<220>
<221> 变体
<222> (15)..(15)
<223> Xaa可以替换为M, V, A
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (23)..(23)
<223> Xaa可以替换为L, M
<400> 22
Phe Leu Gly Phe Met Trp Asn Ser Leu Ser Trp Val Met Glu Xaa Ala
1 5 10 15
Ala Xaa Met Ala Ile Ala Xaa
20
<210> 23
<211> 930
<212> PRT
<213> 拟南芥
<220>
<221> misc_feature
<223> AtAHA5_P50S突变体
<400> 23
Met Glu Glu Val Phe Glu Glu Leu Lys Cys Thr Lys Gln Gly Leu Thr
1 5 10 15
Ala Asn Glu Ala Ser His Arg Leu Asp Val Phe Gly Pro Asn Lys Leu
20 25 30
Glu Glu Lys Lys Glu Ser Lys Leu Leu Lys Phe Leu Gly Phe Met Trp
35 40 45
Asn Ser Leu Ser Trp Val Met Glu Val Ala Ala Leu Met Ala Ile Ala
50 55 60
Leu Ala Asn Gly Gly Gly Arg Pro Pro Asp Trp Gln Asp Phe Val Gly
65 70 75 80
Ile Val Cys Leu Leu Leu Ile Asn Ser Thr Ile Ser Phe Ile Glu Glu
85 90 95
Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Gly Leu Ala Pro
100 105 110
Lys Thr Lys Val Leu Arg Asp Asn Gln Trp Ser Glu Gln Glu Ala Ser
115 120 125
Ile Leu Val Pro Gly Asp Val Ile Ser Ile Lys Leu Gly Asp Ile Ile
130 135 140
Pro Ala Asp Ala Arg Leu Leu Asp Gly Asp Pro Leu Lys Ile Asp Gln
145 150 155 160
Ser Ser Leu Thr Gly Glu Ser Ile Pro Val Thr Lys Asn Pro Ser Asp
165 170 175
Glu Val Phe Ser Gly Ser Ile Cys Lys Gln Gly Glu Ile Glu Ala Ile
180 185 190
Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala His Leu
195 200 205
Val Asp Asn Thr Asn Gln Ile Gly His Phe Gln Lys Val Leu Thr Ser
210 215 220
Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Leu Gly Ile Ile Val Glu
225 230 235 240
Leu Leu Val Met Tyr Pro Ile Gln Arg Arg Arg Tyr Arg Asp Gly Ile
245 250 255
Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala Met Pro
260 265 270
Ser Val Leu Ser Val Thr Met Ala Thr Gly Ser His Arg Leu Phe Gln
275 280 285
Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met Ala Gly
290 295 300
Met Asp Val Leu Cys Cys Asp Lys Thr Gly Thr Leu Thr Leu Asn Lys
305 310 315 320
Leu Thr Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly Val Gly
325 330 335
Lys Glu His Val Phe Leu Leu Ala Ala Arg Ala Ser Arg Ile Glu Asn
340 345 350
Gln Asp Ala Ile Asp Ala Ala Ile Val Gly Met Leu Ala Asp Pro Lys
355 360 365
Glu Ala Arg Ala Gly Val Arg Glu Val His Phe Phe Pro Phe Asn Pro
370 375 380
Val Asp Lys Arg Thr Ala Leu Thr Tyr Val Asp Ser Asp Gly Asn Trp
385 390 395 400
His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Leu Asn Leu Cys Asn
405 410 415
Cys Lys Glu Asp Val Arg Arg Lys Val His Gly Val Ile Asp Lys Phe
420 425 430
Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu Val Leu
435 440 445
Glu Lys Lys Lys Asp Ala Pro Gly Gly Pro Trp Gln Leu Val Gly Leu
450 455 460
Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr Ile Arg
465 470 475 480
Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met Ile Thr Gly Asp Gln
485 490 495
Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly Thr Asn
500 505 510
Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Val Lys Asp Ser Ser Leu
515 520 525
Gly Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala
530 535 540
Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val His Arg Leu Gln Gln
545 550 555 560
Arg Asn His Ile Cys Gly Met Thr Gly Asp Gly Val Asn Asp Ala Pro
565 570 575
Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Val Asp Ala Thr Asp
580 585 590
Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr Glu Pro Gly Leu Ser
595 600 605
Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala Ile Phe Gln Arg Met
610 615 620
Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile Val Phe
625 630 635 640
Gly Phe Met Phe Ile Ala Leu Ile Trp Gln Phe Asp Phe Ser Pro Phe
645 650 655
Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met Thr Ile
660 665 670
Ser Lys Asp Arg Met Lys Pro Ser Pro Gln Pro Asp Ser Trp Lys Leu
675 680 685
Arg Asp Ile Phe Ser Thr Gly Val Val Leu Gly Gly Tyr Gln Ala Leu
690 695 700
Met Thr Val Val Phe Phe Trp Val Met Lys Asp Ser Asp Phe Phe Ser
705 710 715 720
Asn Tyr Phe Gly Val Arg Pro Leu Ser Gln Arg Pro Glu Gln Met Met
725 730 735
Ala Ala Leu Tyr Leu Gln Val Ser Ile Ile Ser Gln Ala Leu Ile Phe
740 745 750
Val Thr Arg Ser Arg Ser Trp Ser Tyr Ala Glu Cys Pro Gly Leu Leu
755 760 765
Leu Leu Gly Ala Phe Val Ile Ala Gln Leu Val Ala Thr Phe Ile Ala
770 775 780
Val Tyr Ala Asn Trp Ser Phe Ala Arg Ile Glu Gly Ala Gly Trp Gly
785 790 795 800
Trp Ala Gly Val Ile Trp Leu Tyr Ser Phe Leu Thr Tyr Ile Pro Leu
805 810 815
Asp Leu Leu Lys Phe Gly Ile Arg Tyr Val Leu Ser Gly Lys Ala Trp
820 825 830
Leu Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr
835 840 845
Gly Lys Glu Glu Arg Glu Ala Gln Trp Ala Ala Ala Gln Arg Thr Leu
850 855 860
His Gly Leu Gln Pro Ala Glu Lys Asn Asn Ile Phe Asn Glu Lys Asn
865 870 875 880
Ser Tyr Ser Glu Leu Ser Gln Ile Ala Glu Gln Ala Lys Arg Arg Ala
885 890 895
Glu Val Val Arg Leu Arg Glu Ile Asn Thr Leu Lys Gly His Val Glu
900 905 910
Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr Ile Gln Gln His
915 920 925
Tyr Thr
930
<210> 24
<211> 107
<212> PRT
<213> 人工的
<220>
<223> 共有序列R1结构域
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为A, S, N
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为S, T, N
<220>
<221> 变体
<222> (49)..(49)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (50)..(50)
<223> Xaa可以替换为N, T
<220>
<221> 变体
<222> (51)..(51)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (53)..(53)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (54)..(54)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (56)..(56)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (59)..(59)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (60)..(60)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (63)..(63)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (74)..(74)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (75)..(75)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (81)..(81)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (82)..(82)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (85)..(85)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (99)..(99)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (102)..(102)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (104)..(104)
<223> Xaa可以替换为N, H
<400> 24
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Trp Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Xaa Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile
50 55 60
Ala Glu Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa
65 70 75 80
Xaa Xaa Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu
85 90 95
Asp Ile Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 25
<211> 106
<212> PRT
<213> 人工的
<220>
<223> 共有序列R1结构域
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为S, T, N
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (49)..(49)
<223> Xaa可以替换为N, T
<220>
<221> 变体
<222> (50)..(50)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (52)..(52)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (53)..(53)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (55)..(55)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (58)..(58)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (59)..(59)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (62)..(62)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (73)..(73)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (74)..(74)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (79)..(79)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (81)..(81)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (84)..(84)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (98)..(98)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (101)..(101)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (103)..(103)
<223> Xaa可以替换为N, H
<400> 25
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Trp Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile Ala
50 55 60
Glu Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa Xaa
65 70 75 80
Xaa Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp
85 90 95
Ile Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 26
<211> 106
<212> PRT
<213> 人工的
<220>
<223> 共有序列R1结构域
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为A, S, N
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (49)..(49)
<223> :Xaa可以替换为N, T
<220>
<221> 变体
<222> (50)..(50)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (52)..(52)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (53)..(53)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (55)..(55)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (58)..(58)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (59)..(59)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (62)..(62)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (73)..(73)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (74)..(74)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (79)..(79)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (81)..(81)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (84)..(84)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (98)..(98)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (101)..(101)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (103)..(103)
<223> Xaa可以替换为N, H
<400> 26
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Trp Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile Ala
50 55 60
Glu Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa Xaa
65 70 75 80
Xaa Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp
85 90 95
Ile Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 27
<211> 105
<212> PRT
<213> 人工的
<220>
<223> 共有序列R1结构域
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为N, T
<220>
<221> 变体
<222> (49)..(49)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (51)..(51)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (52)..(52)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (54)..(54)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (57)..(57)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (58)..(58)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (61)..(61)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (72)..(72)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (73)..(73)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (78)..(78)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (79)..(79)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (83)..(83)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (97)..(97)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (100)..(100)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (102)..(102)
<223> Xaa可以替换为N, H
<400> 27
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Trp Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile Ala Glu
50 55 60
Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa Xaa Xaa
65 70 75 80
Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu Asp Ile
85 90 95
Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 28
<211> 107
<212> PRT
<213> 人工的
<220>
<223> R1结构域在第31位突变 W->L
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为A, S, N
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为S, T, N
<220>
<221> 变体
<222> (49)..(49)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (50)..(50)
<223> Xaa可以替换为N, T
<220>
<221> 变体
<222> (51)..(51)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (53)..(53)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (54)..(54)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (56)..(56)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (59)..(59)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (60)..(60)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (63)..(63)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (74)..(74)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (75)..(75)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (81)..(81)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (82)..(82)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (85)..(85)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (99)..(99)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (102)..(102)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (104)..(104)
<223> Xaa可以替换为N, H
<400> 28
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Leu Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Xaa Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile
50 55 60
Ala Glu Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa
65 70 75 80
Xaa Xaa Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu
85 90 95
Asp Ile Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 29
<211> 107
<212> PRT
<213> 人工的
<220>
<223> R1结构域在第31位突变 W->A
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa可以替换为R, K
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa可以替换为D, L, N, R
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa可以替换为L, M
<220>
<221> 变体
<222> (11)..(11)
<223> Xaa可以替换为E, Q, D
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa可以替换为T, I
<220>
<221> 变体
<222> (18)..(18)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (20)..(20)
<223> Xaa可以替换为K, E
<220>
<221> 变体
<222> (21)..(21)
<223> Xaa可以替换为D, N
<220>
<221> 变体
<222> (24)..(24)
<223> Xaa可以替换为K, R
<220>
<221> 变体
<222> (25)..(25)
<223> Xaa可以替换为E, G
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa可以替换为T, A, L
<220>
<221> 变体
<222> (43)..(43)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (44)..(44)
<223> Xaa可以替换为P, A
<220>
<221> 变体
<222> (46)..(46)
<223> Xaa可以替换为T, V, A, P, K, S
<220>
<221> 变体
<222> (47)..(47)
<223> Xaa可以替换为A, S, N
<220>
<221> 变体
<222> (48)..(48)
<223> Xaa可以替换为S, T, N
<220>
<221> 变体
<222> (49)..(49)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (50)..(50)
<223> Xaa可以替换为N, T
<220>
<221> 变体
<222> (51)..(51)
<223> Xaa可以替换为I, V, M, L
<220>
<221> 变体
<222> (53)..(53)
<223> Xaa可以替换为N, S, H
<220>
<221> 变体
<222> (54)..(54)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (56)..(56)
<223> Xaa可以替换为S, N
<220>
<221> 变体
<222> (59)..(59)
<223> Xaa可以替换为R, S, H
<220>
<221> 变体
<222> (60)..(60)
<223> Xaa可以替换为E, D
<220>
<221> 变体
<222> (63)..(63)
<223> Xaa可以替换为E, Q
<220>
<221> 变体
<222> (74)..(74)
<223> Xaa可以替换为I, V, M
<220>
<221> 变体
<222> (75)..(75)
<223> Xaa可以替换为A, V
<220>
<221> 变体
<222> (80)..(80)
<223> Xaa可以替换为L, I, V
<220>
<221> 变体
<222> (81)..(81)
<223> Xaa可以替换为N, L, H
<220>
<221> 变体
<222> (82)..(82)
<223> Xaa可以替换为T, S
<220>
<221> 变体
<222> (85)..(85)
<223> Xaa可以替换为G, S
<220>
<221> 变体
<222> (99)..(99)
<223> Xaa可以替换为D, E
<220>
<221> 变体
<222> (102)..(102)
<223> Xaa可以替换为Q, N
<220>
<221> 变体
<222> (104)..(104)
<223> Xaa可以替换为N, H
<400> 29
Leu Ser Gly Xaa Ala Trp Xaa Asn Xaa Leu Xaa Asn Lys Xaa Ala Phe
1 5 10 15
Thr Xaa Lys Xaa Xaa Tyr Gly Xaa Xaa Glu Arg Glu Ala Gln Ala Ala
20 25 30
Xaa Ala Gln Arg Thr Leu His Gly Leu Gln Xaa Xaa Glu Xaa Xaa Xaa
35 40 45
Xaa Xaa Xaa Phe Xaa Xaa Lys Xaa Ser Tyr Xaa Xaa Leu Ser Xaa Ile
50 55 60
Ala Glu Gln Ala Lys Arg Arg Ala Glu Xaa Xaa Arg Leu Arg Glu Xaa
65 70 75 80
Xaa Xaa Leu Lys Xaa His Val Glu Ser Val Val Lys Leu Lys Gly Leu
85 90 95
Asp Ile Xaa Thr Ile Xaa Gln Xaa Tyr Thr Val
100 105
<210> 30
<211> 931
<212> PRT
<213> 拟南芥
<220>
<221> misc_feature
<223> AtAHA5_W857L突变体
<400> 30
Met Glu Glu Val Phe Glu Glu Leu Lys Cys Thr Lys Gln Gly Leu Thr
1 5 10 15
Ala Asn Glu Ala Ser His Arg Leu Asp Val Phe Gly Pro Asn Lys Leu
20 25 30
Glu Glu Lys Lys Glu Ser Lys Leu Leu Lys Phe Leu Gly Phe Met Trp
35 40 45
Asn Pro Leu Ser Trp Val Met Glu Val Ala Ala Leu Met Ala Ile Ala
50 55 60
Leu Ala Asn Gly Gly Gly Arg Pro Pro Asp Trp Gln Asp Phe Val Gly
65 70 75 80
Ile Val Cys Leu Leu Leu Ile Asn Ser Thr Ile Ser Phe Ile Glu Glu
85 90 95
Asn Asn Ala Gly Asn Ala Ala Ala Ala Leu Met Ala Gly Leu Ala Pro
100 105 110
Lys Thr Lys Val Leu Arg Asp Asn Gln Trp Ser Glu Gln Glu Ala Ser
115 120 125
Ile Leu Val Pro Gly Asp Val Ile Ser Ile Lys Leu Gly Asp Ile Ile
130 135 140
Pro Ala Asp Ala Arg Leu Leu Asp Gly Asp Pro Leu Lys Ile Asp Gln
145 150 155 160
Ser Ser Leu Thr Gly Glu Ser Ile Pro Val Thr Lys Asn Pro Ser Asp
165 170 175
Glu Val Phe Ser Gly Ser Ile Cys Lys Gln Gly Glu Ile Glu Ala Ile
180 185 190
Val Ile Ala Thr Gly Val His Thr Phe Phe Gly Lys Ala Ala His Leu
195 200 205
Val Asp Asn Thr Asn Gln Ile Gly His Phe Gln Lys Val Leu Thr Ser
210 215 220
Ile Gly Asn Phe Cys Ile Cys Ser Ile Ala Leu Gly Ile Ile Val Glu
225 230 235 240
Leu Leu Val Met Tyr Pro Ile Gln Arg Arg Arg Tyr Arg Asp Gly Ile
245 250 255
Asp Asn Leu Leu Val Leu Leu Ile Gly Gly Ile Pro Ile Ala Met Pro
260 265 270
Ser Val Leu Ser Val Thr Met Ala Thr Gly Ser His Arg Leu Phe Gln
275 280 285
Gln Gly Ala Ile Thr Lys Arg Met Thr Ala Ile Glu Glu Met Ala Gly
290 295 300
Met Asp Val Leu Cys Cys Asp Lys Thr Gly Thr Leu Thr Leu Asn Lys
305 310 315 320
Leu Thr Val Asp Lys Asn Leu Val Glu Val Phe Ala Lys Gly Val Gly
325 330 335
Lys Glu His Val Phe Leu Leu Ala Ala Arg Ala Ser Arg Ile Glu Asn
340 345 350
Gln Asp Ala Ile Asp Ala Ala Ile Val Gly Met Leu Ala Asp Pro Lys
355 360 365
Glu Ala Arg Ala Gly Val Arg Glu Val His Phe Phe Pro Phe Asn Pro
370 375 380
Val Asp Lys Arg Thr Ala Leu Thr Tyr Val Asp Ser Asp Gly Asn Trp
385 390 395 400
His Arg Ala Ser Lys Gly Ala Pro Glu Gln Ile Leu Asn Leu Cys Asn
405 410 415
Cys Lys Glu Asp Val Arg Arg Lys Val His Gly Val Ile Asp Lys Phe
420 425 430
Ala Glu Arg Gly Leu Arg Ser Leu Ala Val Ala Arg Gln Glu Val Leu
435 440 445
Glu Lys Lys Lys Asp Ala Pro Gly Gly Pro Trp Gln Leu Val Gly Leu
450 455 460
Leu Pro Leu Phe Asp Pro Pro Arg His Asp Ser Ala Glu Thr Ile Arg
465 470 475 480
Arg Ala Leu Asn Leu Gly Val Asn Val Lys Met Ile Thr Gly Asp Gln
485 490 495
Leu Ala Ile Gly Lys Glu Thr Gly Arg Arg Leu Gly Met Gly Thr Asn
500 505 510
Met Tyr Pro Ser Ser Ala Leu Leu Gly Gln Val Lys Asp Ser Ser Leu
515 520 525
Gly Ala Leu Pro Val Asp Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala
530 535 540
Gly Val Phe Pro Glu His Lys Tyr Glu Ile Val His Arg Leu Gln Gln
545 550 555 560
Arg Asn His Ile Cys Gly Met Thr Gly Asp Gly Val Asn Asp Ala Pro
565 570 575
Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Val Val Asp Ala Thr Asp
580 585 590
Ala Ala Arg Gly Ala Ser Asp Ile Val Leu Thr Glu Pro Gly Leu Ser
595 600 605
Val Ile Ile Ser Ala Val Leu Thr Ser Arg Ala Ile Phe Gln Arg Met
610 615 620
Lys Asn Tyr Thr Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile Val Phe
625 630 635 640
Gly Phe Met Phe Ile Ala Leu Ile Trp Gln Phe Asp Phe Ser Pro Phe
645 650 655
Met Val Leu Ile Ile Ala Ile Leu Asn Asp Gly Thr Ile Met Thr Ile
660 665 670
Ser Lys Asp Arg Met Lys Pro Ser Pro Gln Pro Asp Ser Trp Lys Leu
675 680 685
Arg Asp Ile Phe Ser Thr Gly Val Val Leu Gly Gly Tyr Gln Ala Leu
690 695 700
Met Thr Val Val Phe Phe Trp Val Met Lys Asp Ser Asp Phe Phe Ser
705 710 715 720
Asn Tyr Phe Gly Val Arg Pro Leu Ser Gln Arg Pro Glu Gln Met Met
725 730 735
Ala Ala Leu Tyr Leu Gln Val Ser Ile Ile Ser Gln Ala Leu Ile Phe
740 745 750
Val Thr Arg Ser Arg Ser Trp Ser Tyr Ala Glu Cys Pro Gly Leu Leu
755 760 765
Leu Leu Gly Ala Phe Val Ile Ala Gln Leu Val Ala Thr Phe Ile Ala
770 775 780
Val Tyr Ala Asn Trp Ser Phe Ala Arg Ile Glu Gly Ala Gly Trp Gly
785 790 795 800
Trp Ala Gly Val Ile Trp Leu Tyr Ser Phe Leu Thr Tyr Ile Pro Leu
805 810 815
Asp Leu Leu Lys Phe Gly Ile Arg Tyr Val Leu Ser Gly Lys Ala Trp
820 825 830
Leu Asn Leu Leu Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr
835 840 845
Gly Lys Glu Glu Arg Glu Ala Gln Leu Ala Ala Ala Gln Arg Thr Leu
850 855 860
His Gly Leu Gln Pro Ala Glu Lys Asn Asn Ile Phe Asn Glu Lys Asn
865 870 875 880
Ser Tyr Ser Glu Leu Ser Gln Ile Ala Glu Gln Ala Lys Arg Arg Ala
885 890 895
Glu Val Val Arg Leu Arg Glu Ile Asn Thr Leu Lys Gly His Val Glu
900 905 910
Ser Val Val Lys Leu Lys Gly Leu Asp Ile Asp Thr Ile Gln Gln His
915 920 925
Tyr Thr Val
930
<210> 31
<211> 961
<212> PRT
<213> 玉米
<220>
<221> misc_feature
<223> ZmAHA5_W885L突变体
<400> 31
Met Gly Pro Leu Gln Arg Arg Pro Thr Ala Met Gly Gly Leu Glu Glu
1 5 10 15
Ile Lys Asn Glu Ala Val Asp Leu Glu Asn Ile Pro Ile Glu Glu Val
20 25 30
Phe Glu Gln Leu Lys Cys Thr Arg Glu Gly Leu Ser Ser Ser Glu Gly
35 40 45
Gln Gln Arg Leu Glu Ile Phe Gly Pro Asn Arg Leu Glu Glu Lys Lys
50 55 60
Glu Ser Lys Ile Leu Lys Phe Leu Gly Phe Met Trp Asn Pro Leu Ser
65 70 75 80
Trp Val Met Glu Met Ala Ala Val Met Ala Ile Ala Leu Ala Asn Gly
85 90 95
Gly Gly Lys Pro Pro Asp Trp Glu Asp Phe Val Gly Ile Ile Val Leu
100 105 110
Leu Val Ile Asn Ser Thr Ile Ser Phe Ile Glu Glu Asn Asn Ala Gly
115 120 125
Asn Ala Ala Ala Ala Leu Met Ala Asn Leu Ala Pro Lys Thr Lys Val
130 135 140
Leu Arg Asp Gly Arg Trp Gly Glu Gln Glu Ala Ala Ile Leu Val Pro
145 150 155 160
Gly Asp Ile Val Ser Ile Lys Leu Gly Asp Ile Val Pro Ala Asp Ala
165 170 175
Arg Leu Leu Glu Gly Asp Pro Leu Lys Val Asp Gln Ser Ala Leu Thr
180 185 190
Gly Glu Ser Leu Pro Val Thr Lys Gly Pro Gly Asp Glu Val Phe Ser
195 200 205
Gly Ser Thr Cys Lys Gln Gly Glu Ile Glu Ala Val Val Ile Ala Thr
210 215 220
Gly Val His Thr Phe Phe Gly Lys Ala Ala His Leu Val Asp Ser Thr
225 230 235 240
Asn Gln Val Gly His Phe Gln Gln Val Leu Thr Ala Ile Gly Asn Phe
245 250 255
Cys Ile Cys Ser Ile Ala Val Gly Ile Val Val Glu Ile Ile Val Met
260 265 270
Phe Pro Ile Gln His Arg Arg Tyr Arg Ser Gly Ile Glu Asn Leu Leu
275 280 285
Val Leu Leu Ile Gly Gly Ile Pro Ile Ala Met Pro Thr Val Leu Ser
290 295 300
Val Thr Met Ala Ile Gly Ser His Lys Leu Ser Gln Gln Gly Ala Ile
305 310 315 320
Thr Lys Arg Met Thr Ala Ile Glu Glu Met Ala Gly Met Asp Val Leu
325 330 335
Cys Ser Asp Lys Thr Gly Thr Leu Thr Leu Asn Lys Leu Ser Val Asp
340 345 350
Lys Asn Leu Val Glu Val Phe Cys Lys Gly Val Asp Lys Asp His Val
355 360 365
Leu Leu Leu Ala Ala Arg Ala Ser Arg Thr Glu Asn Gln Asp Ala Ile
370 375 380
Asp Ala Ala Met Val Gly Met Leu Ala Asp Pro Lys Glu Ala Arg Ala
385 390 395 400
Gly Ile Arg Glu Ile His Phe Leu Pro Phe Asn Pro Val Asp Lys Arg
405 410 415
Thr Ala Leu Thr Tyr Ile Asp Ala Asp Gly His Trp His Arg Val Ser
420 425 430
Lys Gly Ala Pro Glu Gln Ile Leu Asp Leu Cys His Cys Lys Glu Asp
435 440 445
Leu Arg Arg Lys Val His Gly Ile Ile Asp Lys Tyr Ala Glu Arg Gly
450 455 460
Leu Arg Ser Leu Ala Val Ala Arg Gln Glu Val Pro Glu Arg Asn Lys
465 470 475 480
Glu Ser Pro Gly Gly Pro Trp Gln Phe Val Gly Leu Leu Pro Leu Phe
485 490 495
Asp Pro Pro Arg His Asp Ser Ala Glu Thr Ile Arg Lys Ala Leu Val
500 505 510
Leu Gly Val Asn Val Lys Met Ile Thr Gly Asp Gln Leu Ala Ile Gly
515 520 525
Lys Glu Thr Gly Arg Arg Leu Gly Met Gly Thr Asn Met Tyr Pro Ser
530 535 540
Ser Ala Leu Leu Gly Gln Asn Lys Asp Ala Thr Leu Glu Ala Leu Pro
545 550 555 560
Val Asp Glu Leu Ile Glu Lys Ala Asp Gly Phe Ala Gly Val Phe Pro
565 570 575
Glu His Lys Tyr Glu Ile Val Lys Arg Leu Gln Glu Lys Lys His Ile
580 585 590
Val Gly Met Thr Gly Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Lys
595 600 605
Ala Asp Ile Gly Ile Ala Val Ala Asp Ala Thr Asp Ala Ala Arg Ser
610 615 620
Ala Ser Asp Ile Val Leu Thr Glu Pro Gly Leu Ser Val Ile Ile Ser
625 630 635 640
Ala Val Leu Thr Ser Arg Cys Ile Phe Gln Arg Met Lys Asn Tyr Thr
645 650 655
Ile Tyr Ala Val Ser Ile Thr Ile Arg Ile Val Leu Gly Phe Met Leu
660 665 670
Ile Ala Leu Ile Trp Gln Tyr Asp Phe Ser Pro Phe Met Val Leu Ile
675 680 685
Ile Ala Ile Leu Asn Asp Gly Thr Ile Met Thr Ile Ser Lys Asp Arg
690 695 700
Val Lys Pro Ser Pro Leu Pro Asp Ser Trp Lys Leu Lys Glu Ile Phe
705 710 715 720
Ala Thr Gly Ile Val Leu Gly Ser Tyr Leu Ala Leu Met Thr Val Ile
725 730 735
Phe Phe Trp Ala Met His Lys Thr Asp Phe Phe Ser Asp Lys Phe Gly
740 745 750
Val Arg Ser Ile Arg Asp Ser Glu His Glu Met Met Ser Ala Leu Tyr
755 760 765
Leu Gln Val Ser Ile Val Ser Gln Ala Leu Ile Phe Val Thr Arg Ser
770 775 780
Arg Ser Trp Ser Phe Val Glu Arg Pro Gly Leu Leu Leu Val Thr Ala
785 790 795 800
Phe Leu Leu Ala Gln Leu Val Ala Thr Phe Leu Ala Val Tyr Ala Asn
805 810 815
Trp Gly Phe Ala Arg Ile Lys Gly Ile Gly Trp Gly Trp Ala Gly Val
820 825 830
Val Trp Leu Tyr Ser Ile Val Phe Tyr Phe Pro Leu Asp Leu Ile Lys
835 840 845
Phe Phe Ile Arg Phe Val Leu Ser Gly Arg Ala Trp Asp Asn Leu Leu
850 855 860
Glu Asn Lys Thr Ala Phe Thr Thr Lys Lys Asp Tyr Gly Arg Glu Glu
865 870 875 880
Arg Glu Ala Gln Leu Ala Thr Ala Gln Arg Thr Leu His Gly Leu Gln
885 890 895
Pro Pro Glu Ala Ala Thr Ser Thr Leu Phe His Asp Lys Asn Ser Tyr
900 905 910
Arg Glu Leu Ser Glu Ile Ala Glu Gln Ala Lys Arg Arg Ala Glu Ile
915 920 925
Ala Arg Leu Arg Glu Leu Asn Thr Leu Lys Gly His Val Glu Ser Val
930 935 940
Val Lys Leu Lys Gly Leu Asp Ile Asp Thr Ile Gln Gln Asn Tyr Thr
945 950 955 960
Val
<210> 32
<211> 40
<212> DNA
<213> 人工的
<220>
<223> AtAHA5基因的扩增引物
<400> 32
ggattctaga actagtatgg aggaagtgtt cgaggagctg 40
<210> 33
<211> 42
<212> DNA
<213> 人工的
<220>
<223> AtAHA5基因的扩增引物
<400> 33
cggtatcata agcttgttaa acggtgtaat gttgctgaat cg 42
<210> 34
<211> 27
<212> DNA
<213> 人工的
<220>
<223> P50S的定点突变引物
<400> 34
gggttcatgt ggaactcatt gtcgtgg 27
<210> 35
<211> 27
<212> DNA
<213> 人工的
<220>
<223> P50S的定点突变引物
<400> 35
ccacgacaat gagttccaca tgaaccc 27
<210> 36
<211> 29
<212> DNA
<213> 人工的
<220>
<223> W857L的定点突变引物
<400> 36
ggaaagagaa gctcaattgg ctgcagctc 29
<210> 37
<211> 29
<212> DNA
<213> 人工的
<220>
<223> W857L的定点突变引物
<400> 37
gagctgcagc caattgagct tctctttcc 29
<210> 38
<211> 48
<212> DNA
<213> 人工的
<220>
<223> 启动子pAtAHA5的扩增引物
<400> 38
ggggacaact ttgtatagaa aagttgcaac catcatgaca agcgtctg 48
<210> 39
<211> 52
<212> DNA
<213> 人工的
<220>
<223> 启动子pAtAHA5的扩增引物
<400> 39
ggggactgct tttttgtaca aacttggggt attcgtacct agacccatca tt 52
<210> 40
<211> 922
<212> DNA
<213> 双色高粱
<220>
<221> misc_feature
<223> proSbMYB60
<400> 40
ctccgtcgct agcgttgtaa tttttatcag cctctctcca tctgattgga tgagctgctt 60
ctcctagcta gcacgttgtc aaccattggg gacgcccttt aggagtgaaa gtgatgcagt 120
tactctactc ccatccggtc caaatcccaa cctaagggat atatgacgaa gagttttcaa 180
tttcagtata atgtgtatag caagtgattt gattttcctg cagattcttg agagagaagg 240
gccaaacctg catatgtgcc aggtgcgcac tcacagtcac acacacggtc ctcatcaatg 300
cccaagaaat ctggagagac acagacaaac gcaagcaatt actccattca tgagcgccac 360
agcagaaaaa ggagtcctcc agaaccggcc gtgttcttgg cttgcacaca cacacacaca 420
cacgcgaaag cagcgcatgc aacaaagcaa agatcacaca cggccggagc aagagagacg 480
cccatattca tgtgatcacc tggctgactt ttgcattgga aagcaacagt gcgagatcaa 540
aagcgaacgc agcacacagc acagcacagc acagggatcg agttgctcta ctccgtatat 600
gaggtcatgc agtatataag cactcacatg cagctatacc ttagctaggg cacctgtgca 660
aggggacagg taggagataa agcaatgtct gctggctaga ataaaaggag gccgatatcg 720
tatcagcaga gggttccagg ggagaaggcc gttctgcagg gccgccgtaa aaggctgtgt 780
aggaagcagc cacagcttct catatcttct tgttcttcga ctacttgcct agctactata 840
tctactagac tagccctagg ctgcttgcta gctagtaggt gtatcgatcg tccgaggtag 900
gaggagacag caggaagcag gc 922
<210> 41
<211> 773
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> proZmMYB60
<400> 41
ggaagtgttt tgattttcct gcagttctcg atcgagagag gccctcttga atctgaaaga 60
ggtcgagaag ggccagcctg cgtgtcccag gagcgtgcgc tcacacacac ggtcctcatc 120
aatgcccaag aaatctggag agacacaaac aaacgcaaag caattactca cttcatgagc 180
gccacagcag aaaaggagtc ctcaagcagt acgtcaaccg gccgtgttct cgcacacgca 240
aaagcagcgc atgcaacaaa gcaaagatca cacacccgga gcgaagagac gcccattcat 300
gtgatcacct ggctgacttt tgcattggaa agctacagtg cgagatcaaa agcgaacgca 360
gcacagcaca ggccggggga gtggcttata tgaggtcatg cagtatataa gcactcacat 420
gcagctagct aggtagagca cctgtgcatc aaggtaaagc agatggggcc tgctctgctg 480
cctgcctgcc tgctggcatg cgtgtacgtc ctagaataaa aggaggcccg tatcgtatta 540
gcagagggtt ccagggagaa gagaagggag ggcgaggcca ctggctgtgt aggcagcagc 600
cagccacaca gacacagctt cgttcccatc tcttctcgtt cttcatcacc tacttgccta 660
ctatatctac tagccacgct agctagacta gccttagaaa aggctagctc actagctgct 720
tgctaggtgt gtaccgatcg tccgaggtag gagacacagc acgcagcagg ggt 773
<210> 42
<211> 674
<212> DNA
<213> 人工的
<220>
<223> pro35S
<400> 42
attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct 60
atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat 120
tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga 180
cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa 240
gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa aaatatcaaa 300
gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga 360
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 420
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 480
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 540
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 600
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 660
catttggaga ggac 674
<210> 43
<211> 4023
<212> DNA
<213> 人工的
<220>
<223> 35S-AtAHA5P50S盒
<400> 43
attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct 60
atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat 120
tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga 180
cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa 240
gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa aaatatcaaa 300
gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga 360
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 420
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 480
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 540
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 600
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 660
catttggaga ggacctcgac tctagaggat ccccgggtac cgggcccccc ctcgaggcgc 720
gccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc 780
aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccatggag gaagtgttcg 840
aggagctgaa atgtacaaaa caaggtctta cggcaaatga agcttctcac cgtcttgacg 900
tttttggtcc taacaagctt gaagagaaga aggaaagcaa gttacttaag tttttagggt 960
tcatgtggaa ctcattgtcg tgggtgatgg aggtggctgc actaatggca atcgctttgg 1020
ccaacggagg aggacggcca ccggattggc aagactttgt ggggatagtg tgtttactat 1080
taatcaactc aaccattagt ttcattgagg aaaataacgc cggtaatgct gcagccgctc 1140
tcatggccgg tctagcccct aagacaaagg ttctcagaga taatcaatgg agcgagcaag 1200
aagcatcgat tctcgtcccc ggagatgtaa tcagcatcaa gctcggcgat atcattcccg 1260
ccgatgcacg tttactcgac ggcgatcctc tcaaaatcga tcaatcatca ctcaccggtg 1320
aatcaatccc cgtcacaaaa aaccccagcg atgaagtctt ctccggctca atatgcaaac 1380
aaggcgaaat cgaagcaatc gtcatcgcca ctggagtcca cacattcttc ggcaaagcag 1440
ctcatctcgt cgacaacaca aaccaaatcg gccatttcca gaaagttctc acttcgattg 1500
gtaacttctg tatctgttcg attgctttag gaatcatcgt tgagctttta gtaatgtatc 1560
cgattcaacg ccggagatat cgagacggaa tcgataattt acttgttttg ttgatcggag 1620
ggattcctat agctatgcct agtgtgttat ctgttacaat ggcgactggt tctcatagat 1680
tgtttcaaca aggagctatt actaagagaa tgactgctat tgaggaaatg gctggtatgg 1740
atgtattgtg ttgtgataag actggtactc ttacgcttaa taagcttacg gttgataaga 1800
acttagtcga ggttttcgct aaaggagttg gtaaagaaca tgttttcctt ttggctgcga 1860
gagcttcgag gattgagaat caagatgcga ttgatgctgc tattgttgga atgcttgctg 1920
atcctaaaga ggctagagct ggtgttagag aggttcattt tttccctttt aatccggttg 1980
ataagagaac tgctttgact tatgttgact ctgatggaaa ctggcataga gctagtaaag 2040
gagctccaga gcagatactg aacctctgta attgcaagga agatgttagg aggaaagttc 2100
atggagtgat tgataagttt gctgagcgtg gacttcgctc tttggctgtt gcaagacagg 2160
aagttctcga gaagaagaaa gatgctcctg gtggcccatg gcaacttgta ggtcttttac 2220
ctctttttga tcctccgagg catgacagtg ccgagacaat caggagggct ttaaacctcg 2280
gtgttaatgt taagatgata accggggacc agcttgcaat tgggaaagag accggtcgaa 2340
ggcttggaat gggtacgaac atgtatcctt cttctgcgct gctcggacaa gtcaaagatt 2400
cttccttggg tgcacttcct gtggatgaac tgatagagaa ggctgatggg tttgcaggag 2460
tctttccaga acataagtat gagattgttc ataggctgca acaaaggaat cacatatgtg 2520
gcatgactgg agatggtgtg aatgatgccc cggctctcaa gaaggcggat attggtatag 2580
ctgttgttga tgctactgat gctgcgagag gcgcttctga tatcgttctc acagaacccg 2640
gattgagtgt tatcattagt gctgtactca ccagtagagc catattccaa agaatgaaaa 2700
attacactat ttatgcggtt tcaatcacga tacgaattgt gtttggcttc atgttcattg 2760
ccctcatatg gcagttcgat ttttcgcctt tcatggttct gatcatagca atcttaaacg 2820
acggaacaat catgacaata tcaaaagaca gaatgaagcc atctccacag ccagatagct 2880
ggaaactcag agatatattc tcgaccggcg tcgtgcttgg aggttaccag gccttgatga 2940
cagtcgtttt cttctgggtg atgaaagaca gtgatttttt ctcgaactat tttggtgtga 3000
gaccactgag ccaacgtcct gaacaaatga tggctgctct gtatctacaa gttagcatca 3060
taagccaggc tctcatcttc gtcaccagat cccgtagctg gtcctacgcc gaatgccctg 3120
gccttctctt acttggtgca tttgtcatag ctcagctggt ggcgacattt atagcagttt 3180
atgcaaactg gagttttgcc cggatagaag gagccggttg gggatgggct ggagtgatct 3240
ggctatacag tttcctaacg tacatccctc ttgacttact caagtttgga atccgctatg 3300
ttttgagtgg aaaagcttgg ttaaatcttc ttgagaacaa gactgccttc acgacgaaga 3360
aagactatgg gaaagaggaa agagaagctc aatgggctgc agctcaaaga accctccacg 3420
gacttcaacc agcagagaaa aacaacatct ttaacgaaaa gaatagttac agtgaactct 3480
ctcagattgc tgaacaggct aaacgccgag ctgaggttgt caggctgaga gagataaata 3540
cattaaaagg gcatgtagag tcagtggtga agcttaaagg acttgacatt gacacgattc 3600
agcaacatta caccgtttaa aacccagctt tcttgtacaa agttggcatt ataagaaagc 3660
attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 3720
gttaattaac tagttctaga gcggccgccc accgcggtgg agctcgaatt tccccgatcg 3780
ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat 3840
tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac 3900
gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat 3960
agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt 4020
act 4023
<210> 44
<211> 4023
<212> DNA
<213> 人工的
<220>
<223> 35S-AtAHA5W857L盒
<400> 44
attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct 60
atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat 120
tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga 180
cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa 240
gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa aaatatcaaa 300
gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga 360
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 420
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 480
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 540
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 600
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 660
catttggaga ggacctcgac tctagaggat ccccgggtac cgggcccccc ctcgaggcgc 720
gccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc 780
aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccatggag gaagtgttcg 840
aggagctgaa atgtacaaaa caaggtctta cggcaaatga agcttctcac cgtcttgacg 900
tttttggtcc taacaagctt gaagagaaga aggaaagcaa gttacttaag tttttagggt 960
tcatgtggaa cccattgtcg tgggtgatgg aggtggctgc actaatggca atcgctttgg 1020
ccaacggagg aggacggcca ccggattggc aagactttgt ggggatagtg tgtttactat 1080
taatcaactc aaccattagt ttcattgagg aaaataacgc cggtaatgct gcagccgctc 1140
tcatggccgg tctagcccct aagacaaagg ttctcagaga taatcaatgg agcgagcaag 1200
aagcatcgat tctcgtcccc ggagatgtaa tcagcatcaa gctcggcgat atcattcccg 1260
ccgatgcacg tttactcgac ggcgatcctc tcaaaatcga tcaatcatca ctcaccggtg 1320
aatcaatccc cgtcacaaaa aaccccagcg atgaagtctt ctccggctca atatgcaaac 1380
aaggcgaaat cgaagcaatc gtcatcgcca ctggagtcca cacattcttc ggcaaagcag 1440
ctcatctcgt cgacaacaca aaccaaatcg gccatttcca gaaagttctc acttcgattg 1500
gtaacttctg tatctgttcg attgctttag gaatcatcgt tgagctttta gtaatgtatc 1560
cgattcaacg ccggagatat cgagacggaa tcgataattt acttgttttg ttgatcggag 1620
ggattcctat agctatgcct agtgtgttat ctgttacaat ggcgactggt tctcatagat 1680
tgtttcaaca aggagctatt actaagagaa tgactgctat tgaggaaatg gctggtatgg 1740
atgtattgtg ttgtgataag actggtactc ttacgcttaa taagcttacg gttgataaga 1800
acttagtcga ggttttcgct aaaggagttg gtaaagaaca tgttttcctt ttggctgcga 1860
gagcttcgag gattgagaat caagatgcga ttgatgctgc tattgttgga atgcttgctg 1920
atcctaaaga ggctagagct ggtgttagag aggttcattt tttccctttt aatccggttg 1980
ataagagaac tgctttgact tatgttgact ctgatggaaa ctggcataga gctagtaaag 2040
gagctccaga gcagatactg aacctctgta attgcaagga agatgttagg aggaaagttc 2100
atggagtgat tgataagttt gctgagcgtg gacttcgctc tttggctgtt gcaagacagg 2160
aagttctcga gaagaagaaa gatgctcctg gtggcccatg gcaacttgta ggtcttttac 2220
ctctttttga tcctccgagg catgacagtg ccgagacaat caggagggct ttaaacctcg 2280
gtgttaatgt taagatgata accggggacc agcttgcaat tgggaaagag accggtcgaa 2340
ggcttggaat gggtacgaac atgtatcctt cttctgcgct gctcggacaa gtcaaagatt 2400
cttccttggg tgcacttcct gtggatgaac tgatagagaa ggctgatggg tttgcaggag 2460
tctttccaga acataagtat gagattgttc ataggctgca acaaaggaat cacatatgtg 2520
gcatgactgg agatggtgtg aatgatgccc cggctctcaa gaaggcggat attggtatag 2580
ctgttgttga tgctactgat gctgcgagag gcgcttctga tatcgttctc acagaacccg 2640
gattgagtgt tatcattagt gctgtactca ccagtagagc catattccaa agaatgaaaa 2700
attacactat ttatgcggtt tcaatcacga tacgaattgt gtttggcttc atgttcattg 2760
ccctcatatg gcagttcgat ttttcgcctt tcatggttct gatcatagca atcttaaacg 2820
acggaacaat catgacaata tcaaaagaca gaatgaagcc atctccacag ccagatagct 2880
ggaaactcag agatatattc tcgaccggcg tcgtgcttgg aggttaccag gccttgatga 2940
cagtcgtttt cttctgggtg atgaaagaca gtgatttttt ctcgaactat tttggtgtga 3000
gaccactgag ccaacgtcct gaacaaatga tggctgctct gtatctacaa gttagcatca 3060
taagccaggc tctcatcttc gtcaccagat cccgtagctg gtcctacgcc gaatgccctg 3120
gccttctctt acttggtgca tttgtcatag ctcagctggt ggcgacattt atagcagttt 3180
atgcaaactg gagttttgcc cggatagaag gagccggttg gggatgggct ggagtgatct 3240
ggctatacag tttcctaacg tacatccctc ttgacttact caagtttgga atccgctatg 3300
ttttgagtgg aaaagcttgg ttaaatcttc ttgagaacaa gactgccttc acgacgaaga 3360
aagactatgg gaaagaggaa agagaagctc aattggctgc agctcaaaga accctccacg 3420
gacttcaacc agcagagaaa aacaacatct ttaacgaaaa gaatagttac agtgaactct 3480
ctcagattgc tgaacaggct aaacgccgag ctgaggttgt caggctgaga gagataaata 3540
cattaaaagg gcatgtagag tcagtggtga agcttaaagg acttgacatt gacacgattc 3600
agcaacatta caccgtttaa aacccagctt tcttgtacaa agttggcatt ataagaaagc 3660
attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 3720
gttaattaac tagttctaga gcggccgccc accgcggtgg agctcgaatt tccccgatcg 3780
ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat 3840
tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac 3900
gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat 3960
agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt 4020
act 4023
<210> 45
<211> 4023
<212> DNA
<213> 人工的
<220>
<223> 35S-AtAHA5野生型盒
<400> 45
attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct 60
atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat 120
tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga 180
cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa 240
gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa aaatatcaaa 300
gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga 360
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 420
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 480
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 540
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 600
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 660
catttggaga ggacctcgac tctagaggat ccccgggtac cgggcccccc ctcgaggcgc 720
gccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc 780
aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccatggag gaagtgttcg 840
aggagctgaa atgtacaaaa caaggtctta cggcaaatga agcttctcac cgtcttgacg 900
tttttggtcc taacaagctt gaagagaaga aggaaagcaa gttacttaag tttttagggt 960
tcatgtggaa cccattgtcg tgggtgatgg aggtggctgc actaatggca atcgctttgg 1020
ccaacggagg aggacggcca ccggattggc aagactttgt ggggatagtg tgtttactat 1080
taatcaactc aaccattagt ttcattgagg aaaataacgc cggtaatgct gcagccgctc 1140
tcatggccgg tctagcccct aagacaaagg ttctcagaga taatcaatgg agcgagcaag 1200
aagcatcgat tctcgtcccc ggagatgtaa tcagcatcaa gctcggcgat atcattcccg 1260
ccgatgcacg tttactcgac ggcgatcctc tcaaaatcga tcaatcatca ctcaccggtg 1320
aatcaatccc cgtcacaaaa aaccccagcg atgaagtctt ctccggctca atatgcaaac 1380
aaggcgaaat cgaagcaatc gtcatcgcca ctggagtcca cacattcttc ggcaaagcag 1440
ctcatctcgt cgacaacaca aaccaaatcg gccatttcca gaaagttctc acttcgattg 1500
gtaacttctg tatctgttcg attgctttag gaatcatcgt tgagctttta gtaatgtatc 1560
cgattcaacg ccggagatat cgagacggaa tcgataattt acttgttttg ttgatcggag 1620
ggattcctat agctatgcct agtgtgttat ctgttacaat ggcgactggt tctcatagat 1680
tgtttcaaca aggagctatt actaagagaa tgactgctat tgaggaaatg gctggtatgg 1740
atgtattgtg ttgtgataag actggtactc ttacgcttaa taagcttacg gttgataaga 1800
acttagtcga ggttttcgct aaaggagttg gtaaagaaca tgttttcctt ttggctgcga 1860
gagcttcgag gattgagaat caagatgcga ttgatgctgc tattgttgga atgcttgctg 1920
atcctaaaga ggctagagct ggtgttagag aggttcattt tttccctttt aatccggttg 1980
ataagagaac tgctttgact tatgttgact ctgatggaaa ctggcataga gctagtaaag 2040
gagctccaga gcagatactg aacctctgta attgcaagga agatgttagg aggaaagttc 2100
atggagtgat tgataagttt gctgagcgtg gacttcgctc tttggctgtt gcaagacagg 2160
aagttctcga gaagaagaaa gatgctcctg gtggcccatg gcaacttgta ggtcttttac 2220
ctctttttga tcctccgagg catgacagtg ccgagacaat caggagggct ttaaacctcg 2280
gtgttaatgt taagatgata accggggacc agcttgcaat tgggaaagag accggtcgaa 2340
ggcttggaat gggtacgaac atgtatcctt cttctgcgct gctcggacaa gtcaaagatt 2400
cttccttggg tgcacttcct gtggatgaac tgatagagaa ggctgatggg tttgcaggag 2460
tctttccaga acataagtat gagattgttc ataggctgca acaaaggaat cacatatgtg 2520
gcatgactgg agatggtgtg aatgatgccc cggctctcaa gaaggcggat attggtatag 2580
ctgttgttga tgctactgat gctgcgagag gcgcttctga tatcgttctc acagaacccg 2640
gattgagtgt tatcattagt gctgtactca ccagtagagc catattccaa agaatgaaaa 2700
attacactat ttatgcggtt tcaatcacga tacgaattgt gtttggcttc atgttcattg 2760
ccctcatatg gcagttcgat ttttcgcctt tcatggttct gatcatagca atcttaaacg 2820
acggaacaat catgacaata tcaaaagaca gaatgaagcc atctccacag ccagatagct 2880
ggaaactcag agatatattc tcgaccggcg tcgtgcttgg aggttaccag gccttgatga 2940
cagtcgtttt cttctgggtg atgaaagaca gtgatttttt ctcgaactat tttggtgtga 3000
gaccactgag ccaacgtcct gaacaaatga tggctgctct gtatctacaa gttagcatca 3060
taagccaggc tctcatcttc gtcaccagat cccgtagctg gtcctacgcc gaatgccctg 3120
gccttctctt acttggtgca tttgtcatag ctcagctggt ggcgacattt atagcagttt 3180
atgcaaactg gagttttgcc cggatagaag gagccggttg gggatgggct ggagtgatct 3240
ggctatacag tttcctaacg tacatccctc ttgacttact caagtttgga atccgctatg 3300
ttttgagtgg aaaagcttgg ttaaatcttc ttgagaacaa gactgccttc acgacgaaga 3360
aagactatgg gaaagaggaa agagaagctc aatgggctgc agctcaaaga accctccacg 3420
gacttcaacc agcagagaaa aacaacatct ttaacgaaaa gaatagttac agtgaactct 3480
ctcagattgc tgaacaggct aaacgccgag ctgaggttgt caggctgaga gagataaata 3540
cattaaaagg gcatgtagag tcagtggtga agcttaaagg acttgacatt gacacgattc 3600
agcaacatta caccgtttaa aacccagctt tcttgtacaa agttggcatt ataagaaagc 3660
attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 3720
gttaattaac tagttctaga gcggccgccc accgcggtgg agctcgaatt tccccgatcg 3780
ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat 3840
tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac 3900
gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat 3960
agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt 4020
act 4023
<210> 46
<211> 2356
<212> DNA
<213> 拟南芥
<220>
<221> misc_feature
<223> pAtAHA5
<400> 46
aaccatcatg acaagcgtct ggatttttgg aatctaggat tttttgaaca tatagtgtgg 60
tgattttttg gggagaagag atcattgttg atgtatgttg cctgagttat ggccgcgacg 120
aaaaaataga ctgaaatgtg tgaaaagatg gtataaatat ctttacctag gtttctaggt 180
tttgattact gggcatgtga aatgggtttt aataaatggg cttgggcttg taaaattgtg 240
gaattaagct attgtaatca cgcatatgag ttatctaatt ggataactta ttagattgta 300
ataatgaggg aaaaaaaaag attattataa tgagaggtcc acatttaaaa tgatgtgaca 360
ctcttagcaa ataaatataa catggcaaaa tttagaagtg ttgtcaaaaa acgataagcg 420
taaaatggaa attgtgactg aaccaccata agaagcgtct ggatttttgg agtccgagag 480
atttagaaag tggagtgtgg ttgttttttt tagaggggga atgttgttgt ttatgccatt 540
agagtcatgg tggcgtcgaa gaacaataag tatatgtggg aatcagagat gtgtgtaaga 600
gaaacaaaaa tttatttggt tgggttttga attttcaaga aaatgttaaa ttatttcatc 660
gagtttcttt ttttttctct atgttaggaa atggtatggt caaaatctat tgatgatggt 720
gatgcctaac attaacaaaa ccaatagaag ggagacaaga gacataagac atcttcttga 780
aattcatcaa cattacaaac ttactaaaac tcctcctccc aatcccaaat gaatatgtac 840
ttcttaaagc cacgtgtctt tctctttttc attaatattt atttattttt ttaaaatgtg 900
aaattatttc tttggtttaa tatctctgga tcattcttct acaatgatca caagtattta 960
gatcatgtgt aaatagtccc atcatgaagt tttaattacc aatcggtatt gcacatcacc 1020
gattggtact gatccacctg cacttttgtc gatcttttgg gacaacgaaa aagaaactaa 1080
tttaatatat tttgttatcc gctcgaagat attaattata agctttaatt tggaacttct 1140
ttatatcaat tatatatata tataacttct ttgttaatag aaaatgggaa cgatccaaaa 1200
cttaagacat ttgatctcca caaggccgtc gacgtacaga gaagaagaat ggccaaaaat 1260
aagtaaataa aagagaagaa accgagggga agatgatttt gcattgccac gacccacgag 1320
catccatcgg ccatcgcctt tttggattca catggcaaag acaaacttcg atcttccttt 1380
tttctcgtga agttattatc tctctaattt ctagtctggt cgaacctata tttcatcata 1440
tatatcatta tttaattcat atgctgatgc atgggacatt atcatgttaa gtcattacaa 1500
aaaaataaaa ataaaaataa cttacgttgg actctcagtt taccttctcc tatggatttc 1560
acttccttgt ataagaatat acatcttact caaatcatga tttatatgca attttctttt 1620
gtgagatttt ttttacatat aaaccagaaa tgttgaaaga agaatatcca aacacaacaa 1680
aataatagaa gaaatctcac gtagaacatg gaaatggaaa aagaagtgaa ctcgtgaacc 1740
gatatattta ccaaaagtgc acacatagac aagtctttct cattaaagcc caatttcaca 1800
ctttcacatt ttcgtgacgt cttttgaagt tttgaatacg aattaatgtt catgtacgct 1860
ataaagacct aaaaggttgt catatttctc cactttcatc ttctcttcac aactatccta 1920
aacctttttt ccttttcttc ttcttcactc tctttcttcc cggcgtcact atggcggctg 1980
ccgccgggta aaggcagcga actcgaccac atcaagaatg aatccgttga tctggtatat 2040
atatttacgt cttcttagtt ttctattatc tatatgtttt aaaaaatctt tggccctact 2100
aattttaaat tttctgttac ataaaagata catatatgta tagtacgtaa ctagtaacta 2160
ctttgatcta atcagttaat atatttgtcc catgcgattt actcatgttt ccattagaaa 2220
tgattagtca aaattatata aatgatgggt ctaggtacga ataccctaat atatttgtcc 2280
catgcgattt actcatgttt ccattagaaa tgattagtca aaattatata aatgatgggt 2340
ctaggtacga ataccc 2356
<210> 47
<211> 6253
<212> DNA
<213> 人工的
<220>
<223> pAtAHA5-AtAHA5W857L盒
<400> 47
aaccatcatg acaagcgtct ggatttttgg aatctaggat tttttgaaca tatagtgtgg 60
tgattttttg gggagaagag atcattgttg atgtatgttg cctgagttat ggccgcgacg 120
aaaaaataga ctgaaatgtg tgaaaagatg gtataaatat ctttacctag gtttctaggt 180
tttgattact gggcatgtga aatgggtttt aataaatggg cttgggcttg taaaattgtg 240
gaattaagct attgtaatca cgcatatgag ttatctaatt ggataactta ttagattgta 300
ataatgaggg aaaaaaaaag attattataa tgagaggtcc acatttaaaa tgatgtgaca 360
ctcttagcaa ataaatataa catggcaaaa tttagaagtg ttgtcaaaaa acgataagcg 420
taaaatggaa attgtgactg aaccaccata agaagcgtct ggatttttgg agtccgagag 480
atttagaaag tggagtgtgg ttgttttttt tagaggggga atgttgttgt ttatgccatt 540
agagtcatgg tggcgtcgaa gaacaataag tatatgtggg aatcagagat gtgtgtaaga 600
gaaacaaaaa tttatttggt tgggttttga attttcaaga aaatgttaaa ttatttcatc 660
gagtttcttt ttttttctct atgttaggaa atggtatggt caaaatctat tgatgatggt 720
gatgcctaac attaacaaaa ccaatagaag ggagacaaga gacataagac atcttcttga 780
aattcatcaa cattacaaac ttactaaaac tcctcctccc aatcccaaat gaatatgtac 840
ttcttaaagc cacgtgtctt tctctttttc attaatattt atttattttt ttaaaatgtg 900
aaattatttc tttggtttaa tatctctgga tcattcttct acaatgatca caagtattta 960
gatcatgtgt aaatagtccc atcatgaagt tttaattacc aatcggtatt gcacatcacc 1020
gattggtact gatccacctg cacttttgtc gatcttttgg gacaacgaaa aagaaactaa 1080
tttaatatat tttgttatcc gctcgaagat attaattata agctttaatt tggaacttct 1140
ttatatcaat tatatatata tataacttct ttgttaatag aaaatgggaa cgatccaaaa 1200
cttaagacat ttgatctcca caaggccgtc gacgtacaga gaagaagaat ggccaaaaat 1260
aagtaaataa aagagaagaa accgagggga agatgatttt gcattgccac gacccacgag 1320
catccatcgg ccatcgcctt tttggattca catggcaaag acaaacttcg atcttccttt 1380
tttctcgtga agttattatc tctctaattt ctagtctggt cgaacctata tttcatcata 1440
tatatcatta tttaattcat atgctgatgc atgggacatt atcatgttaa gtcattacaa 1500
aaaaataaaa ataaaaataa cttacgttgg actctcagtt taccttctcc tatggatttc 1560
acttccttgt ataagaatat acatcttact caaatcatga tttatatgca attttctttt 1620
gtgagatttt ttttacatat aaaccagaaa tgttgaaaga agaatatcca aacacaacaa 1680
aataatagaa gaaatctcac gtagaacatg gaaatggaaa aagaagtgaa ctcgtgaacc 1740
gatatattta ccaaaagtgc acacatagac aagtctttct cattaaagcc caatttcaca 1800
ctttcacatt ttcgtgacgt cttttgaagt tttgaatacg aattaatgtt catgtacgct 1860
ataaagacct aaaaggttgt catatttctc cactttcatc ttctcttcac aactatccta 1920
aacctttttt ccttttcttc ttcttcactc tctttcttcc cggcgtcact atggcggctg 1980
ccgccgggta aaggcagcga actcgaccac atcaagaatg aatccgttga tctggtatat 2040
atatttacgt cttcttagtt ttctattatc tatatgtttt aaaaaatctt tggccctact 2100
aattttaaat tttctgttac ataaaagata catatatgta tagtacgtaa ctagtaacta 2160
ctttgatcta atcagttaat atatttgtcc catgcgattt actcatgttt ccattagaaa 2220
tgattagtca aaattatata aatgatgggt ctaggtacga ataccctaat atatttgtcc 2280
catgcgattt actcatgttt ccattagaaa tgattagtca aaattatata aatgatgggt 2340
ctaggtacga atacccatgc aagtttgtac aaaaaagcag gcttaatggt gagcaagggc 2400
gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2460
cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2520
aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2580
acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2640
aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 2700
aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2760
ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2820
tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 2880
ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 2940
aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 3000
tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 3060
accgccgccg ggatcactct cggcatggac gagctgtaca agtacccagc tttcttgtac 3120
aaagtggcta tggaggaagt gttcgaggag ctgaaatgta caaaacaagg tcttacggca 3180
aatgaagctt ctcaccgtct tgacgttttt ggtcctaaca agcttgaaga gaagaaggaa 3240
agcaagttac ttaagttttt agggttcatg tggaacccat tgtcgtgggt gatggaggtg 3300
gctgcactaa tggcaatcgc tttggccaac ggaggaggac ggccaccgga ttggcaagac 3360
tttgtgggga tagtgtgttt actattaatc aactcaacca ttagtttcat tgaggaaaat 3420
aacgccggta atgctgcagc cgctctcatg gccggtctag cccctaagac aaaggttctc 3480
agagataatc aatggagcga gcaagaagca tcgattctcg tccccggaga tgtaatcagc 3540
atcaagctcg gcgatatcat tcccgccgat gcacgtttac tcgacggcga tcctctcaaa 3600
atcgatcaat catcactcac cggtgaatca atccccgtca caaaaaaccc cagcgatgaa 3660
gtcttctccg gctcaatatg caaacaaggc gaaatcgaag caatcgtcat cgccactgga 3720
gtccacacat tcttcggcaa agcagctcat ctcgtcgaca acacaaacca aatcggccat 3780
ttccagaaag ttctcacttc gattggtaac ttctgtatct gttcgattgc tttaggaatc 3840
atcgttgagc ttttagtaat gtatccgatt caacgccgga gatatcgaga cggaatcgat 3900
aatttacttg ttttgttgat cggagggatt cctatagcta tgcctagtgt gttatctgtt 3960
acaatggcga ctggttctca tagattgttt caacaaggag ctattactaa gagaatgact 4020
gctattgagg aaatggctgg tatggatgta ttgtgttgtg ataagactgg tactcttacg 4080
cttaataagc ttacggttga taagaactta gtcgaggttt tcgctaaagg agttggtaaa 4140
gaacatgttt tccttttggc tgcgagagct tcgaggattg agaatcaaga tgcgattgat 4200
gctgctattg ttggaatgct tgctgatcct aaagaggcta gagctggtgt tagagaggtt 4260
cattttttcc cttttaatcc ggttgataag agaactgctt tgacttatgt tgactctgat 4320
ggaaactggc atagagctag taaaggagct ccagagcaga tactgaacct ctgtaattgc 4380
aaggaagatg ttaggaggaa agttcatgga gtgattgata agtttgctga gcgtggactt 4440
cgctctttgg ctgttgcaag acaggaagtt ctcgagaaga agaaagatgc tcctggtggc 4500
ccatggcaac ttgtaggtct tttacctctt tttgatcctc cgaggcatga cagtgccgag 4560
acaatcagga gggctttaaa cctcggtgtt aatgttaaga tgataaccgg ggaccagctt 4620
gcaattggga aagagaccgg tcgaaggctt ggaatgggta cgaacatgta tccttcttct 4680
gcgctgctcg gacaagtcaa agattcttcc ttgggtgcac ttcctgtgga tgaactgata 4740
gagaaggctg atgggtttgc aggagtcttt ccagaacata agtatgagat tgttcatagg 4800
ctgcaacaaa ggaatcacat atgtggcatg actggagatg gtgtgaatga tgccccggct 4860
ctcaagaagg cggatattgg tatagctgtt gttgatgcta ctgatgctgc gagaggcgct 4920
tctgatatcg ttctcacaga acccggattg agtgttatca ttagtgctgt actcaccagt 4980
agagccatat tccaaagaat gaaaaattac actatttatg cggtttcaat cacgatacga 5040
attgtgtttg gcttcatgtt cattgccctc atatggcagt tcgatttttc gcctttcatg 5100
gttctgatca tagcaatctt aaacgacgga acaatcatga caatatcaaa agacagaatg 5160
aagccatctc cacagccaga tagctggaaa ctcagagata tattctcgac cggcgtcgtg 5220
cttggaggtt accaggcctt gatgacagtc gttttcttct gggtgatgaa agacagtgat 5280
tttttctcga actattttgg tgtgagacca ctgagccaac gtcctgaaca aatgatggct 5340
gctctgtatc tacaagttag catcataagc caggctctca tcttcgtcac cagatcccgt 5400
agctggtcct acgccgaatg ccctggcctt ctcttacttg gtgcatttgt catagctcag 5460
ctggtggcga catttatagc agtttatgca aactggagtt ttgcccggat agaaggagcc 5520
ggttggggat gggctggagt gatctggcta tacagtttcc taacgtacat ccctcttgac 5580
ttactcaagt ttggaatccg ctatgttttg agtggaaaag cttggttaaa tcttcttgag 5640
aacaagactg ccttcacgac gaagaaagac tatgggaaag aggaaagaga agctcaattg 5700
gctgcagctc aaagaaccct ccacggactt caaccagcag agaaaaacaa catctttaac 5760
gaaaagaata gttacagtga actctctcag attgctgaac aggctaaacg ccgagctgag 5820
gttgtcaggc tgagagagat aaatacatta aaagggcatg tagagtcagt ggtgaagctt 5880
aaaggacttg acattgacac gattcagcaa cattacaccg tttaacaact ttattataca 5940
tagttgataa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac gatctaactg 6000
actagccgcg gccatggcgg ccgggagcgg ccatgctaga gtccgcaaaa atcaccagtc 6060
tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag 6120
ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt 6180
agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 6240
aatccagtga cct 6253
<210> 48
<211> 6253
<212> DNA
<213> 人工的
<220>
<223> pAtAHA5-AtAHA5野生型盒
<400> 48
aaccatcatg acaagcgtct ggatttttgg aatctaggat tttttgaaca tatagtgtgg 60
tgattttttg gggagaagag atcattgttg atgtatgttg cctgagttat ggccgcgacg 120
aaaaaataga ctgaaatgtg tgaaaagatg gtataaatat ctttacctag gtttctaggt 180
tttgattact gggcatgtga aatgggtttt aataaatggg cttgggcttg taaaattgtg 240
gaattaagct attgtaatca cgcatatgag ttatctaatt ggataactta ttagattgta 300
ataatgaggg aaaaaaaaag attattataa tgagaggtcc acatttaaaa tgatgtgaca 360
ctcttagcaa ataaatataa catggcaaaa tttagaagtg ttgtcaaaaa acgataagcg 420
taaaatggaa attgtgactg aaccaccata agaagcgtct ggatttttgg agtccgagag 480
atttagaaag tggagtgtgg ttgttttttt tagaggggga atgttgttgt ttatgccatt 540
agagtcatgg tggcgtcgaa gaacaataag tatatgtggg aatcagagat gtgtgtaaga 600
gaaacaaaaa tttatttggt tgggttttga attttcaaga aaatgttaaa ttatttcatc 660
gagtttcttt ttttttctct atgttaggaa atggtatggt caaaatctat tgatgatggt 720
gatgcctaac attaacaaaa ccaatagaag ggagacaaga gacataagac atcttcttga 780
aattcatcaa cattacaaac ttactaaaac tcctcctccc aatcccaaat gaatatgtac 840
ttcttaaagc cacgtgtctt tctctttttc attaatattt atttattttt ttaaaatgtg 900
aaattatttc tttggtttaa tatctctgga tcattcttct acaatgatca caagtattta 960
gatcatgtgt aaatagtccc atcatgaagt tttaattacc aatcggtatt gcacatcacc 1020
gattggtact gatccacctg cacttttgtc gatcttttgg gacaacgaaa aagaaactaa 1080
tttaatatat tttgttatcc gctcgaagat attaattata agctttaatt tggaacttct 1140
ttatatcaat tatatatata tataacttct ttgttaatag aaaatgggaa cgatccaaaa 1200
cttaagacat ttgatctcca caaggccgtc gacgtacaga gaagaagaat ggccaaaaat 1260
aagtaaataa aagagaagaa accgagggga agatgatttt gcattgccac gacccacgag 1320
catccatcgg ccatcgcctt tttggattca catggcaaag acaaacttcg atcttccttt 1380
tttctcgtga agttattatc tctctaattt ctagtctggt cgaacctata tttcatcata 1440
tatatcatta tttaattcat atgctgatgc atgggacatt atcatgttaa gtcattacaa 1500
aaaaataaaa ataaaaataa cttacgttgg actctcagtt taccttctcc tatggatttc 1560
acttccttgt ataagaatat acatcttact caaatcatga tttatatgca attttctttt 1620
gtgagatttt ttttacatat aaaccagaaa tgttgaaaga agaatatcca aacacaacaa 1680
aataatagaa gaaatctcac gtagaacatg gaaatggaaa aagaagtgaa ctcgtgaacc 1740
gatatattta ccaaaagtgc acacatagac aagtctttct cattaaagcc caatttcaca 1800
ctttcacatt ttcgtgacgt cttttgaagt tttgaatacg aattaatgtt catgtacgct 1860
ataaagacct aaaaggttgt catatttctc cactttcatc ttctcttcac aactatccta 1920
aacctttttt ccttttcttc ttcttcactc tctttcttcc cggcgtcact atggcggctg 1980
ccgccgggta aaggcagcga actcgaccac atcaagaatg aatccgttga tctggtatat 2040
atatttacgt cttcttagtt ttctattatc tatatgtttt aaaaaatctt tggccctact 2100
aattttaaat tttctgttac ataaaagata catatatgta tagtacgtaa ctagtaacta 2160
ctttgatcta atcagttaat atatttgtcc catgcgattt actcatgttt ccattagaaa 2220
tgattagtca aaattatata aatgatgggt ctaggtacga ataccctaat atatttgtcc 2280
catgcgattt actcatgttt ccattagaaa tgattagtca aaattatata aatgatgggt 2340
ctaggtacga atacccatgc aagtttgtac aaaaaagcag gcttaatggt gagcaagggc 2400
gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2460
cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2520
aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2580
acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2640
aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 2700
aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2760
ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2820
tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 2880
ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 2940
aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 3000
tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 3060
accgccgccg ggatcactct cggcatggac gagctgtaca agtacccagc tttcttgtac 3120
aaagtggcta tggaggaagt gttcgaggag ctgaaatgta caaaacaagg tcttacggca 3180
aatgaagctt ctcaccgtct tgacgttttt ggtcctaaca agcttgaaga gaagaaggaa 3240
agcaagttac ttaagttttt agggttcatg tggaacccat tgtcgtgggt gatggaggtg 3300
gctgcactaa tggcaatcgc tttggccaac ggaggaggac ggccaccgga ttggcaagac 3360
tttgtgggga tagtgtgttt actattaatc aactcaacca ttagtttcat tgaggaaaat 3420
aacgccggta atgctgcagc cgctctcatg gccggtctag cccctaagac aaaggttctc 3480
agagataatc aatggagcga gcaagaagca tcgattctcg tccccggaga tgtaatcagc 3540
atcaagctcg gcgatatcat tcccgccgat gcacgtttac tcgacggcga tcctctcaaa 3600
atcgatcaat catcactcac cggtgaatca atccccgtca caaaaaaccc cagcgatgaa 3660
gtcttctccg gctcaatatg caaacaaggc gaaatcgaag caatcgtcat cgccactgga 3720
gtccacacat tcttcggcaa agcagctcat ctcgtcgaca acacaaacca aatcggccat 3780
ttccagaaag ttctcacttc gattggtaac ttctgtatct gttcgattgc tttaggaatc 3840
atcgttgagc ttttagtaat gtatccgatt caacgccgga gatatcgaga cggaatcgat 3900
aatttacttg ttttgttgat cggagggatt cctatagcta tgcctagtgt gttatctgtt 3960
acaatggcga ctggttctca tagattgttt caacaaggag ctattactaa gagaatgact 4020
gctattgagg aaatggctgg tatggatgta ttgtgttgtg ataagactgg tactcttacg 4080
cttaataagc ttacggttga taagaactta gtcgaggttt tcgctaaagg agttggtaaa 4140
gaacatgttt tccttttggc tgcgagagct tcgaggattg agaatcaaga tgcgattgat 4200
gctgctattg ttggaatgct tgctgatcct aaagaggcta gagctggtgt tagagaggtt 4260
cattttttcc cttttaatcc ggttgataag agaactgctt tgacttatgt tgactctgat 4320
ggaaactggc atagagctag taaaggagct ccagagcaga tactgaacct ctgtaattgc 4380
aaggaagatg ttaggaggaa agttcatgga gtgattgata agtttgctga gcgtggactt 4440
cgctctttgg ctgttgcaag acaggaagtt ctcgagaaga agaaagatgc tcctggtggc 4500
ccatggcaac ttgtaggtct tttacctctt tttgatcctc cgaggcatga cagtgccgag 4560
acaatcagga gggctttaaa cctcggtgtt aatgttaaga tgataaccgg ggaccagctt 4620
gcaattggga aagagaccgg tcgaaggctt ggaatgggta cgaacatgta tccttcttct 4680
gcgctgctcg gacaagtcaa agattcttcc ttgggtgcac ttcctgtgga tgaactgata 4740
gagaaggctg atgggtttgc aggagtcttt ccagaacata agtatgagat tgttcatagg 4800
ctgcaacaaa ggaatcacat atgtggcatg actggagatg gtgtgaatga tgccccggct 4860
ctcaagaagg cggatattgg tatagctgtt gttgatgcta ctgatgctgc gagaggcgct 4920
tctgatatcg ttctcacaga acccggattg agtgttatca ttagtgctgt actcaccagt 4980
agagccatat tccaaagaat gaaaaattac actatttatg cggtttcaat cacgatacga 5040
attgtgtttg gcttcatgtt cattgccctc atatggcagt tcgatttttc gcctttcatg 5100
gttctgatca tagcaatctt aaacgacgga acaatcatga caatatcaaa agacagaatg 5160
aagccatctc cacagccaga tagctggaaa ctcagagata tattctcgac cggcgtcgtg 5220
cttggaggtt accaggcctt gatgacagtc gttttcttct gggtgatgaa agacagtgat 5280
tttttctcga actattttgg tgtgagacca ctgagccaac gtcctgaaca aatgatggct 5340
gctctgtatc tacaagttag catcataagc caggctctca tcttcgtcac cagatcccgt 5400
agctggtcct acgccgaatg ccctggcctt ctcttacttg gtgcatttgt catagctcag 5460
ctggtggcga catttatagc agtttatgca aactggagtt ttgcccggat agaaggagcc 5520
ggttggggat gggctggagt gatctggcta tacagtttcc taacgtacat ccctcttgac 5580
ttactcaagt ttggaatccg ctatgttttg agtggaaaag cttggttaaa tcttcttgag 5640
aacaagactg ccttcacgac gaagaaagac tatgggaaag aggaaagaga agctcaatgg 5700
gctgcagctc aaagaaccct ccacggactt caaccagcag agaaaaacaa catctttaac 5760
gaaaagaata gttacagtga actctctcag attgctgaac aggctaaacg ccgagctgag 5820
gttgtcaggc tgagagagat aaatacatta aaagggcatg tagagtcagt ggtgaagctt 5880
aaaggacttg acattgacac gattcagcaa cattacaccg tttaacaact ttattataca 5940
tagttgataa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac gatctaactg 6000
actagccgcg gccatggcgg ccgggagcgg ccatgctaga gtccgcaaaa atcaccagtc 6060
tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag 6120
ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt 6180
agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 6240
aatccagtga cct 6253
<210> 49
<211> 2886
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> ZmAHA5W885L version 1
<400> 49
atggggccac tgcagcggcg gcctacggct atgggggggc tggaggagat taagaacgag 60
gcggttgacc tggagaacat cccgattgag gaggtgttcg agcagctgaa gtgcacccgc 120
gagggcctct ccagctcgga ggggcagcag cgcctggaga tcttcggccc caaccgcctg 180
gaggagaaga aggagtccaa gatcctcaag ttcctgggct tcatgtggaa cccactgagc 240
tgggttatgg agatggctgc cgtgatggct atcgcgctcg ctaatggcgg gggcaagcca 300
ccagactggg aggatttcgt cggcatcatt gtgctcctgg tcatcaactc gacgatttct 360
ttcatcgagg agaacaatgc tggcaacgct gccgcggctc tgatggctaa tctcgctcct 420
aagacaaagg tgctgaggga tggccgctgg ggggagcagg aggccgcgat cctggtgccg 480
ggcgacattg tcagcatcaa gctcggggac atcgttccag ctgatgctag gctcctggag 540
ggggacccac tcaaggtgga tcagagcgct ctgacgggcg agtcgctccc tgtcacaaag 600
gggccaggcg acgaggtttt ctccggcagc acgtgcaagc agggggagat tgaggccgtg 660
gtcatcgcga ctggcgtcca caccttcttc gggaaggctg cccatctggt cgactcgact 720
aaccaggttg gccacttcca gcaggtgctc accgcgatcg ggaatttctg catttgctct 780
atcgctgtgg gcattgttgt ggagatcatt gtcatgttcc caatccagca taggcgctac 840
cgctccggca ttgagaacct cctggtgctc ctgatcgggg gcatccccat tgcgatgcca 900
acggttctga gcgtgacaat ggccatcggc agccacaagc tctcgcagca gggggccatt 960
acgaagagga tgacagccat cgaggagatg gcggggatgg acgtcctgtg ctctgataag 1020
acaggcactc tcaccctgaa caagctgtca gtggacaaga atctcgttga ggtgttctgc 1080
aagggcgttg acaaggatca cgtgctcctg ctcgcggctc gggcctcccg caccgagaac 1140
caggacgcga tcgatgctgc gatggtgggg atgctcgccg atccaaagga ggctagggcc 1200
ggcattcggg agatccattt cctgcccttc aatccagtcg acaagaggac tgctctcacc 1260
tacatcgacg ccgatggcca ctggcatcgg gtgagcaagg gggccccaga gcagatcctc 1320
gacctgtgcc actgcaagga ggatctgcgg cgcaaggtgc atggcatcat tgacaagtac 1380
gcggagaggg ggctcaggtc gctggcggtc gctcggcagg aggttcccga gcgcaacaag 1440
gagtctcctg ggggcccgtg gcagttcgtc ggcctgctcc cactcttcga cccacctcgg 1500
cacgattccg cggagactat ccgcaaggct ctcgtgctgg gcgtcaatgt taagatgatt 1560
accggggatc agctggccat cggcaaggag acggggagga ggctcgggat gggcacaaac 1620
atgtacccgt cttcagcgct gctcggccag aataaggacg ctaccctgga ggccctcccg 1680
gtggacgagc tgatcgagaa ggcggatggc ttcgctgggg tcttcccgga gcacaagtac 1740
gagattgtta agaggctgca ggagaagaag catatcgtgg gcatgacggg ggacggcgtc 1800
aacgatgctc ctgctctcaa gaaggcggat atcggcattg ctgtcgctga cgctacggat 1860
gctgctcggt cggcctctga catcgttctg acagagccgg gcctctctgt gatcatttca 1920
gcggtcctga cctcacgctg cattttccag aggatgaaga actacactat ctacgcggtt 1980
tccatcacca ttcgcatcgt gctcggcttc atgctcattg ccctgatctg gcagtacgac 2040
ttctctccgt tcatggtcct gatcattgcc atcctcaatg acggcacgat tatgacaatc 2100
tcaaaggata gggtgaagcc atcgcctctg ccggactctt ggaagctcaa ggagattttc 2160
gccacaggca tcgtgctggg gtcctacctc gctctgatga ctgtcatctt cttctgggcc 2220
atgcacaaga ccgacttctt cagcgataag ttcggcgtcc gctccatcag ggacagcgag 2280
catgagatga tgtcggcgct ctacctgcag gtctcaattg tttcccaggc cctcatcttc 2340
gtgacccggt cacgctcctg gagcttcgtt gagaggccag gcctgctcct ggtgacggcc 2400
ttcctcctgg cgcagctggt cgctacattc ctcgccgttt acgcgaactg gggcttcgcg 2460
cgcattaagg ggatcggctg ggggtgggct ggcgtcgttt ggctgtactc catcgtcttc 2520
tacttcccgc tcgacctgat taagttcttc atcaggttcg tgctcagcgg ccgggcgtgg 2580
gataacctcc tggagaataa gacggccttc accacgaaga aggactacgg cagggaggag 2640
cgcgaggctc agctcgctac tgctcagcgc accctccacg gcctgcagcc accagaggct 2700
gctacgtcga cactgttcca tgacaagaac tcatacaggg agctctccga gattgctgag 2760
caggccaagc gcagggcgga gatcgctagg ctcagggagc tgaacaccct caaggggcac 2820
gtcgagtccg tggtcaagct gaagggcctc gacattgata ctatccagca gaattacacc 2880
gtgtag 2886
<210> 50
<211> 2886
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> ZmAHA5W885L version 2
<400> 50
atgggcccac tgcagaggcg cccaacagcg atggggggcc tggaggagat caagaacgag 60
gccgtcgatc tggagaacat ccctattgag gaggtgttcg agcagctgaa gtgcacccgg 120
gagggcctca gctcctcgga gggccagcag cgcctggaga tcttcggccc aaaccggctg 180
gaggagaaga aggagagcaa gatcctcaag ttcctggggt tcatgtggaa cccgctgtcc 240
tgggtcatgg agatggctgc cgtgatggcg attgctctgg ctaatggcgg gggcaagcca 300
ccggactggg aggatttcgt ggggattatc gtcctgctcg tcatcaacag cactatctcc 360
ttcattgagg agaacaacgc tggcaacgcc gctgctgcgc tgatggcgaa cctcgctcca 420
aagactaagg tcctccgcga cggccgctgg ggggagcagg aggctgcgat tctcgtgccc 480
ggggacatcg ttagcattaa gctcggggat atcgtccctg ctgatgcccg gctgctggag 540
ggggaccccc tgaaggtcga ccagagcgct ctcaccggcg agtcgctccc agtcactaag 600
ggccctgggg acgaggtgtt cagcggctct acgtgcaagc agggcgagat cgaggcggtc 660
gtgatcgcta ccggcgtgca caccttcttc gggaaggccg cccatctcgt ggattcgaca 720
aaccaggtcg ggcacttcca gcaggtgctg acagccatcg ggaacttctg catctgctcg 780
attgccgttg ggatcgtggt tgagattatt gtgatgttcc cgattcagca ccggcgctac 840
cgctccggga tcgagaacct cctggtcctc ctcattgggg ggattccgat cgctatgccg 900
accgtcctga gcgtcactat ggctatcggg tctcacaagc tctcacagca gggggcgatt 960
accaagcgga tgacagcgat tgaggagatg gcggggatgg acgtgctgtg ctcagacaag 1020
accggcactc tgacgctcaa caagctctcc gtggacaaga acctcgtcga ggtgttctgc 1080
aagggggtgg acaaggatca tgtcctgctg ctggcggcca gggcctcccg gacagagaac 1140
caggacgcca ttgacgccgc tatggtgggg atgctggccg accctaagga ggcgagggcc 1200
ggcatccgcg agattcactt cctgccattc aatcccgttg acaagcgcac tgcgctgacg 1260
tacattgatg cggacgggca ctggcacagg gtgtccaagg gggctccaga gcagatcctc 1320
gatctgtgcc actgcaagga ggatctgcgg cgcaaggtgc atgggatcat tgacaagtac 1380
gcggagaggg ggctccggag cctggcggtg gccaggcagg aggtgccgga gcgcaacaag 1440
gagtcgccgg ggggcccttg gcagttcgtc gggctgctcc ctctgttcga tcccccgagg 1500
cacgacagcg cggagactat ccgcaaggcc ctcgttctgg gggtgaatgt caagatgatt 1560
accggggacc agctggccat tgggaaggag acaggccggc gcctcgggat gggcacgaac 1620
atgtacccgt catccgccct gctggggcag aataaggacg ccactctgga ggccctgccg 1680
gtcgatgagc tcatcgagaa ggctgacggg ttcgcgggcg tcttccccga gcataagtac 1740
gagatcgtga agcggctgca ggagaagaag catattgtgg ggatgactgg cgacggggtg 1800
aatgatgctc ccgccctgaa gaaggctgac atcggcattg ccgtcgcgga cgccacagat 1860
gctgcccgct cggcgtccga tatcgttctc acggagcctg ggctgagcgt tattatctcg 1920
gcggttctga caagcaggtg catcttccag cgcatgaaga actacaccat ctacgctgtg 1980
tcgatcacta tcaggatcgt gctgggcttc atgctgattg cgctcatctg gcagtacgac 2040
ttctcccctt tcatggtcct gatcattgct atcctcaacg acggcacaat catgactatc 2100
tcgaaggacc gcgtgaagcc gagcccactc cccgactctt ggaagctcaa ggagattttc 2160
gccactggga tcgttctcgg gtcttacctc gctctgatga ccgtgatttt cttctgggct 2220
atgcacaaga ccgacttctt cagcgacaag ttcggggttc gctcgattcg cgactcggag 2280
cacgagatga tgtctgcgct gtacctccag gtttcaattg tttcacaggc gctcattttc 2340
gtgactaggt cacggtcttg gtccttcgtc gagaggcctg gcctgctcct ggtcactgct 2400
ttcctgctcg cccagctggt ggccactttc ctcgctgttt acgctaattg ggggttcgct 2460
aggatcaagg ggatcggctg ggggtgggcg ggcgtggtct ggctctactc catcgttttc 2520
tacttcccac tcgacctcat caagttcttc attcggttcg tgctgtcagg gagggcgtgg 2580
gacaacctcc tggagaacaa gaccgcgttc accactaaga aggactacgg ccgcgaggag 2640
agggaggcgc agctggctac agcgcagcgc accctccacg ggctccagcc cccagaggct 2700
gcgacatcta cactcttcca cgacaagaat tcttaccgcg agctgtcaga gattgccgag 2760
caggctaagc gcagggcgga gattgctagg ctgagggagc tcaacacact gaagggccac 2820
gtggagtcag tggtcaagct gaagggcctg gacatcgata caattcagca gaactacacc 2880
gtttag 2886
<210> 51
<211> 504
<212> DNA
<213> 木薯脉花叶病毒
<220>
<221> misc_feature
<223> proCsVMV
<400> 51
aaggtaatta tccaagatgt agcatcaaga atccaatgtt tacgggaaaa actatggaag 60
tattatgtga gctcagcaag aagcagatca atatgcggca catatgcaac ctatgttcaa 120
aaatgaagaa tgtacagata caagatccta tactgccaga atacgaagaa gaatacgtag 180
aaattgaaaa agaagaacca ggcgaagaaa agaatcttga agacgtaagc actgacgaca 240
acaatgaaaa gaagaagata aggtcggtga ttgtgaaaga gacatagagg acacatgtaa 300
ggtggaaaat gtaagggcgg aaagtaacct tatcacaaag gaatcttatc ccccactact 360
tatcctttta tatttttccg tgtcattttt gcccttgagt tttcctatat aaggaaccaa 420
gttcggcatt tgtgaaaaca agaaaaaatt tggtgtaagc tattttcttt gaagtactga 480
ggatacaact tcagagaaat ttgt 504
<210> 52
<211> 557
<212> DNA
<213> 人工的
<220>
<223> 大米肌动蛋白内含子
<400> 52
accaccacca ccaccaccac ctccacctcc tcccccctcg ctgccggacg acgagctcct 60
cccccctccc cctccgccgc cgccgcgccg gtaaccaccc cgcccctctc ctctttcttt 120
ctccgttttt tttttccgtc tcggtctcga tctttggcct tggtagtttg ggtgggcgag 180
aggcggcttc gtgcgcgccc agatcggtgc gcgggagggg cgggatctcg cggctggggc 240
tctcgccggc gtggatccgg cccggatctc gcggggaatg gggctctcgg atgtagatct 300
gcgatccgcc gttgttgggg gagatgatgg ggggtttaaa atttccgcca tgctaaacaa 360
gatcaggaag aggggaaaag ggcactatgg tttatatttt tatatatttc tgctgcttcg 420
tcaggcttag atgtgctaga tctttctttc ttctttttgt gggtagaatt tgaatccctc 480
agcattgttc atcggtagtt tttcttttca tgatttgtga caaatgcagc ctcgtgcgga 540
gcttttttgt aggtaga 557
<210> 53
<211> 509
<212> DNA
<213> 拟南芥
<220>
<221> misc_feature
<223> terSac66
<400> 53
ctaaatgctc ttaactgagc taattatgta atgcacatac acatatttac atagatatgc 60
atatttatat atagcatgta tattgtacta catgcattgc ttcttaatac atgtagtaaa 120
gatatatgca aaaatagtcg aaagatttgt ttacatataa aatcaccaat atttattgtt 180
attgtatttt catgaataaa gtaataagat tatttgtcta atattttgat ttactagtac 240
tagaaatgaa aaggaatatg cacaatttca gcattatagt ttggtaggca aaatggagtg 300
agaatagagt ttcatagtat atactaaggt tcttaattgt gcaaatagtt gatacaagtc 360
acatgggcca agtttgtaaa tcttaaatcg aaatatgcct tcttcttttt ttgcatgaaa 420
atgctagtaa tttataagtg tgtttttcaa taagagatgc taaataccaa aattaaccta 480
gttttcagtg agcgcttgca ttattgtgg 509
<210> 54
<211> 968
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> proZmrbcS_Mut_al
<400> 54
tccctttaat ctggcgctag atctgcatcc gcggcttgca aagataaatg gcacatttag 60
tgtgttattt tgcaatacct ttcatagtag atatccttaa atgcagtttt aggcatgttt 120
gggtaattaa ataacatttt taggaggagt tttagattta cctttctttc gtgatgactg 180
atgacagacg tggggaattc aaatgcaact ctagcgaaag ttcatatatt tttcataaat 240
agctgaggct ggggtaatta ttttttttgt agaaaaatag aataggtgga atggttgggg 300
aaggcgtagg cgctcgtgga cgacgcccga taaaagacaa gaggcggaat tgccatgaat 360
tcgaggtagc taagtaaggc gcatatatat gccaaaaaat tctactgtca ctttccaatt 420
tcaatgcgct gccaaacaag ccatcctgga aactgacttg aattcagccc aattctgtag 480
atccaaacag ggccggcgtc agtgcctcag gtgagagagc agcagacgat tcaaagagcc 540
aaaactgcaa gcagacgcag ccgaagccga agccgaagcc caagcccaaa actgttttgt 600
ctttgcccag aaccgcgacg agcctaaact gcgcttcctc ctatctacaa gtccctggca 660
catcacgcat agtccaacct aggcgcgcag gcgataaggc gcgccacggg gacgcgacat 720
gtggtggcgg acgcgatcag gatagggcca ggctggccgg gcgcggccac gggagaacgg 780
tggccactcg tcccacatcc gcttcgtcct gtcctgtact gcgtcctgcc cccaacgaga 840
gccggagccg gccatcccgt cgcacactct ccccctctat atatgccgtc ggtgtggggg 900
agcctactac aggacgaccc aagcaagcaa gcaagcagcg agtacataca tactaggcag 960
ccaggcag 968
<210> 55
<211> 537
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> terZmRbcs
<400> 55
tagaccgcgc ccgccggccg ccccccgccg gctagctagc tagctagcta gctcctgcgt 60
gagctagtag ctagtgccat gcgtcgtctc tgtcgttcgg ttttgcttcg ggtcaccgta 120
ccctttgctt gcttggtttc ttctttcctt ttttcctttt ttttttcttc ttttccccgg 180
ccatggttcc tttgctttca gcagttctct gctggatgtg atgtatccat tgttgcaagc 240
atggccttgc attggctacc tctatacctg ctacaaacta ctgcaacgcc tatatatact 300
tggggtgagg aacatgtgaa tgcaagctcc ggctatcata tacatgtaat atggatacaa 360
actatatata taaatccgcc gaggcgccga ctaatactat acgacgacac cgtgttaagt 420
taatatataa ctggtgcttt ttatttatat atctgtctca tcatatatat atgctaatta 480
atggatgtgt gtcctcttca cttcaattcc ttctttcctt tcctatgctt tgagatc 537
<210> 56
<211> 444
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> proZmRAB17_del
<400> 56
aaatgttatg cagttcgctc tggacttttc tgctgcgcct acacttgggt gtactgggcc 60
taaattcagc ctgaccgacc gcctgcattg aataatggat gagcaccggt aaaatccgcg 120
tacccaactt tcgagaagaa ccgagacgtg gcgggccggg ccaccgacgc acggcaccag 180
cgactgcaca cgtcccgccg gcgtacgtgt acgtgctgtt ccctcactgg ccgcccaatc 240
cactcatgca tgcccacgta cacccctgcc gtggcgcgcc cagatcctaa tcctttcgcc 300
gttctgcact tctgctgcct ataaatggcg gcatcgaccg tcacctgctt caccaccggc 360
gagccacatc gagaacacga tcgagcacac aagcacgaag actcgtttag gagaaaccac 420
aaaccaccaa gccgtgcaag catc 444
<210> 57
<211> 500
<212> DNA
<213> 玉米
<220>
<221> misc_feature
<223> terZmRab17
<400> 57
gcggcgccta tacgtggctg tgctgtgctg tctggcgcgt caaagccgta ctcttcagcg 60
ttccatagat aataagataa acccatgaat aagtgtccct accctttgat catgtgacag 120
ggacagggac agtttcctgt acttgtgggg tgaatttgta cgtgtgatac ggtaacttgg 180
tccgtgtact gtttatgctg ctacgtttcc ttcctaaaag tacgtatata tatacagcac 240
ttgcgagtgg ctttacttgt gtgcactgcc taaaagtgtc gatattacct ccagcggtaa 300
ataaatgacc cttgtaagaa acttacacag catgtcttac taattaacag ccgttattat 360
gttcggcgca gtactcacac tggaacttac gaacaagaat tgctctatca atcaccatct 420
actttagata tatcttctga aacttttttc aaatccaaaa gttcagtatc ttcctacaaa 480
ttagtgaatt aggaatccaa 500
<210> 58
<211> 4416
<212> DNA
<213> 人工的
<220>
<223> 盒_T02781_CsVMV ZmAHA5_W885L
<400> 58
aaggtaatta tccaagatgt agcatcaaga atccaatgtt tacgggaaaa actatggaag 60
tattatgtga gctcagcaag aagcagatca atatgcggca catatgcaac ctatgttcaa 120
aaatgaagaa tgtacagata caagatccta tactgccaga atacgaagaa gaatacgtag 180
aaattgaaaa agaagaacca ggcgaagaaa agaatcttga agacgtaagc actgacgaca 240
acaatgaaaa gaagaagata aggtcggtga ttgtgaaaga gacatagagg acacatgtaa 300
ggtggaaaat gtaagggcgg aaagtaacct tatcacaaag gaatcttatc ccccactact 360
tatcctttta tatttttccg tgtcattttt gcccttgagt tttcctatat aaggaaccaa 420
gttcggcatt tgtgaaaaca agaaaaaatt tggtgtaagc tattttcttt gaagtactga 480
ggatacaact tcagagaaat ttgtccctcc cccctccccc tccgccgccg ccgcgccggt 540
aaccaccccg cccctctcct ctttctttct ccgttttttt tttccgtctc ggtctcgatc 600
tttggccttg gtagtttggg tgggcgagag gcggcttcgt gcgcgcccag atcggtgcgc 660
gggaggggcg ggatctcgcg gctggggctc tcgccggcgt ggatccggcc cggatctcgc 720
ggggaatggg gctctcggat gtagatctgc gatccgccgt tgttggggga gatgatgggg 780
ggtttaaaat ttccgccatg ctaaacaaga tcaggaagag gggaaaaggg cactatggtt 840
tatattttta tatatttctg ctgcttcgtc aggcttagat gtgctagatc tttctttctt 900
ctttttgtgg gtagaatttg aatccctcag cattgttcat cggtagtttt tcttttcatg 960
atttgtgaca aatgcagcct cgtgcggagc ttttttgtag gtagacgata tctccaccat 1020
ggggccactg cagcggcggc ctacggctat gggggggctg gaggagatta agaacgaggc 1080
ggttgacctg gagaacatcc cgattgagga ggtgttcgag cagctgaagt gcacccgcga 1140
gggcctctcc agctcggagg ggcagcagcg cctggagatc ttcggcccca accgcctgga 1200
ggagaagaag gagtccaaga tcctcaagtt cctgggcttc atgtggaacc cactgagctg 1260
ggttatggag atggctgccg tgatggctat cgcgctcgct aatggcgggg gcaagccacc 1320
agactgggag gatttcgtcg gcatcattgt gctcctggtc atcaactcga cgatttcttt 1380
catcgaggag aacaatgctg gcaacgctgc cgcggctctg atggctaatc tcgctcctaa 1440
gacaaaggtg ctgagggatg gccgctgggg ggagcaggag gccgcgatcc tggtgccggg 1500
cgacattgtc agcatcaagc tcggggacat cgttccagct gatgctaggc tcctggaggg 1560
ggacccactc aaggtggatc agagcgctct gacgggcgag tcgctccctg tcacaaaggg 1620
gccaggcgac gaggttttct ccggcagcac gtgcaagcag ggggagattg aggccgtggt 1680
catcgcgact ggcgtccaca ccttcttcgg gaaggctgcc catctggtcg actcgactaa 1740
ccaggttggc cacttccagc aggtgctcac cgcgatcggg aatttctgca tttgctctat 1800
cgctgtgggc attgttgtgg agatcattgt catgttccca atccagcata ggcgctaccg 1860
ctccggcatt gagaacctcc tggtgctcct gatcgggggc atccccattg cgatgccaac 1920
ggttctgagc gtgacaatgg ccatcggcag ccacaagctc tcgcagcagg gggccattac 1980
gaagaggatg acagccatcg aggagatggc ggggatggac gtcctgtgct ctgataagac 2040
aggcactctc accctgaaca agctgtcagt ggacaagaat ctcgttgagg tgttctgcaa 2100
gggcgttgac aaggatcacg tgctcctgct cgcggctcgg gcctcccgca ccgagaacca 2160
ggacgcgatc gatgctgcga tggtggggat gctcgccgat ccaaaggagg ctagggccgg 2220
cattcgggag atccatttcc tgcccttcaa tccagtcgac aagaggactg ctctcaccta 2280
catcgacgcc gatggccact ggcatcgggt gagcaagggg gccccagagc agatcctcga 2340
cctgtgccac tgcaaggagg atctgcggcg caaggtgcat ggcatcattg acaagtacgc 2400
ggagaggggg ctcaggtcgc tggcggtcgc tcggcaggag gttcccgagc gcaacaagga 2460
gtctcctggg ggcccgtggc agttcgtcgg cctgctccca ctcttcgacc cacctcggca 2520
cgattccgcg gagactatcc gcaaggctct cgtgctgggc gtcaatgtta agatgattac 2580
cggggatcag ctggccatcg gcaaggagac ggggaggagg ctcgggatgg gcacaaacat 2640
gtacccgtct tcagcgctgc tcggccagaa taaggacgct accctggagg ccctcccggt 2700
ggacgagctg atcgagaagg cggatggctt cgctggggtc ttcccggagc acaagtacga 2760
gattgttaag aggctgcagg agaagaagca tatcgtgggc atgacggggg acggcgtcaa 2820
cgatgctcct gctctcaaga aggcggatat cggcattgct gtcgctgacg ctacggatgc 2880
tgctcggtcg gcctctgaca tcgttctgac agagccgggc ctctctgtga tcatttcagc 2940
ggtcctgacc tcacgctgca ttttccagag gatgaagaac tacactatct acgcggtttc 3000
catcaccatt cgcatcgtgc tcggcttcat gctcattgcc ctgatctggc agtacgactt 3060
ctctccgttc atggtcctga tcattgccat cctcaatgac ggcacgatta tgacaatctc 3120
aaaggatagg gtgaagccat cgcctctgcc ggactcttgg aagctcaagg agattttcgc 3180
cacaggcatc gtgctggggt cctacctcgc tctgatgact gtcatcttct tctgggccat 3240
gcacaagacc gacttcttca gcgataagtt cggcgtccgc tccatcaggg acagcgagca 3300
tgagatgatg tcggcgctct acctgcaggt ctcaattgtt tcccaggccc tcatcttcgt 3360
gacccggtca cgctcctgga gcttcgttga gaggccaggc ctgctcctgg tgacggcctt 3420
cctcctggcg cagctggtcg ctacattcct cgccgtttac gcgaactggg gcttcgcgcg 3480
cattaagggg atcggctggg ggtgggctgg cgtcgtttgg ctgtactcca tcgtcttcta 3540
cttcccgctc gacctgatta agttcttcat caggttcgtg ctcagcggcc gggcgtggga 3600
taacctcctg gagaataaga cggccttcac cacgaagaag gactacggca gggaggagcg 3660
cgaggctcag ctcgctactg ctcagcgcac cctccacggc ctgcagccac cagaggctgc 3720
tacgtcgaca ctgttccatg acaagaactc atacagggag ctctccgaga ttgctgagca 3780
ggccaagcgc agggcggaga tcgctaggct cagggagctg aacaccctca aggggcacgt 3840
cgagtccgtg gtcaagctga agggcctcga cattgatact atccagcaga attacaccgt 3900
gtagaaccta aatgctctta actgagctaa ttatgtaatg cacatacaca tatttacata 3960
gatatgcata tttatatata gcatgtatat tgtactacat gcattgcttc ttaatacatg 4020
tagtaaagat atatgcaaaa atagtcgaaa gatttgttta catataaaat caccaatatt 4080
tattgttatt gtattttcat gaataaagta ataagattat ttgtctaata ttttgattta 4140
ctagtactag aaatgaaaag gaatatgcac aatttcagca ttatagtttg gtaggcaaaa 4200
tggagtgaga atagagtttc atagtatata ctaaggttct taattgtgca aatagttgat 4260
acaagtcaca tgggccaagt ttgtaaatct taaatcgaaa tatgccttct tctttttttg 4320
catgaaaatg ctagtaattt ataagtgtgt ttttcaataa gagatgctaa ataccaaaat 4380
taacctagtt ttcagtgagc gcttgcatta ttgtgg 4416
<210> 59
<211> 4402
<212> DNA
<213> 人工的
<220>
<223> 盒_T02784_rbcS_Mut_al ZmAHA5_W885L
<400> 59
tccctttaat ctggcgctag atctgcatcc gcggcttgca aagataaatg gcacatttag 60
tgtgttattt tgcaatacct ttcatagtag atatccttaa atgcagtttt aggcatgttt 120
gggtaattaa ataacatttt taggaggagt tttagattta cctttctttc gtgatgactg 180
atgacagacg tggggaattc aaatgcaact ctagcgaaag ttcatatatt tttcataaat 240
agctgaggct ggggtaatta ttttttttgt agaaaaatag aataggtgga atggttgggg 300
aaggcgtagg cgctcgtgga cgacgcccga taaaagacaa gaggcggaat tgccatgaat 360
tcgaggtagc taagtaaggc gcatatatat gccaaaaaat tctactgtca ctttccaatt 420
tcaatgcgct gccaaacaag ccatcctgga aactgacttg aattcagccc aattctgtag 480
atccaaacag ggccggcgtc agtgcctcag gtgagagagc agcagacgat tcaaagagcc 540
aaaactgcaa gcagacgcag ccgaagccga agccgaagcc caagcccaaa actgttttgt 600
ctttgcccag aaccgcgacg agcctaaact gcgcttcctc ctatctacaa gtccctggca 660
catcacgcat agtccaacct aggcgcgcag gcgataaggc gcgccacggg gacgcgacat 720
gtggtggcgg acgcgatcag gatagggcca ggctggccgg gcgcggccac gggagaacgg 780
tggccactcg tcccacatcc gcttcgtcct gtcctgtact gcgtcctgcc cccaacgaga 840
gccggagccg gccatcccgt cgcacactct ccccctctat atatgccgtc ggtgtggggg 900
agcctactac aggacgaccc aagcaagcaa gcaagcagcg agtacataca tactaggcag 960
ccaggcagtc tccaccatgg ggccactgca gcggcggcct acggctatgg gggggctgga 1020
ggagattaag aacgaggcgg ttgacctgga gaacatcccg attgaggagg tgttcgagca 1080
gctgaagtgc acccgcgagg gcctctccag ctcggagggg cagcagcgcc tggagatctt 1140
cggccccaac cgcctggagg agaagaagga gtccaagatc ctcaagttcc tgggcttcat 1200
gtggaaccca ctgagctggg ttatggagat ggctgccgtg atggctatcg cgctcgctaa 1260
tggcgggggc aagccaccag actgggagga tttcgtcggc atcattgtgc tcctggtcat 1320
caactcgacg atttctttca tcgaggagaa caatgctggc aacgctgccg cggctctgat 1380
ggctaatctc gctcctaaga caaaggtgct gagggatggc cgctgggggg agcaggaggc 1440
cgcgatcctg gtgccgggcg acattgtcag catcaagctc ggggacatcg ttccagctga 1500
tgctaggctc ctggaggggg acccactcaa ggtggatcag agcgctctga cgggcgagtc 1560
gctccctgtc acaaaggggc caggcgacga ggttttctcc ggcagcacgt gcaagcaggg 1620
ggagattgag gccgtggtca tcgcgactgg cgtccacacc ttcttcggga aggctgccca 1680
tctggtcgac tcgactaacc aggttggcca cttccagcag gtgctcaccg cgatcgggaa 1740
tttctgcatt tgctctatcg ctgtgggcat tgttgtggag atcattgtca tgttcccaat 1800
ccagcatagg cgctaccgct ccggcattga gaacctcctg gtgctcctga tcgggggcat 1860
ccccattgcg atgccaacgg ttctgagcgt gacaatggcc atcggcagcc acaagctctc 1920
gcagcagggg gccattacga agaggatgac agccatcgag gagatggcgg ggatggacgt 1980
cctgtgctct gataagacag gcactctcac cctgaacaag ctgtcagtgg acaagaatct 2040
cgttgaggtg ttctgcaagg gcgttgacaa ggatcacgtg ctcctgctcg cggctcgggc 2100
ctcccgcacc gagaaccagg acgcgatcga tgctgcgatg gtggggatgc tcgccgatcc 2160
aaaggaggct agggccggca ttcgggagat ccatttcctg cccttcaatc cagtcgacaa 2220
gaggactgct ctcacctaca tcgacgccga tggccactgg catcgggtga gcaagggggc 2280
cccagagcag atcctcgacc tgtgccactg caaggaggat ctgcggcgca aggtgcatgg 2340
catcattgac aagtacgcgg agagggggct caggtcgctg gcggtcgctc ggcaggaggt 2400
tcccgagcgc aacaaggagt ctcctggggg cccgtggcag ttcgtcggcc tgctcccact 2460
cttcgaccca cctcggcacg attccgcgga gactatccgc aaggctctcg tgctgggcgt 2520
caatgttaag atgattaccg gggatcagct ggccatcggc aaggagacgg ggaggaggct 2580
cgggatgggc acaaacatgt acccgtcttc agcgctgctc ggccagaata aggacgctac 2640
cctggaggcc ctcccggtgg acgagctgat cgagaaggcg gatggcttcg ctggggtctt 2700
cccggagcac aagtacgaga ttgttaagag gctgcaggag aagaagcata tcgtgggcat 2760
gacgggggac ggcgtcaacg atgctcctgc tctcaagaag gcggatatcg gcattgctgt 2820
cgctgacgct acggatgctg ctcggtcggc ctctgacatc gttctgacag agccgggcct 2880
ctctgtgatc atttcagcgg tcctgacctc acgctgcatt ttccagagga tgaagaacta 2940
cactatctac gcggtttcca tcaccattcg catcgtgctc ggcttcatgc tcattgccct 3000
gatctggcag tacgacttct ctccgttcat ggtcctgatc attgccatcc tcaatgacgg 3060
cacgattatg acaatctcaa aggatagggt gaagccatcg cctctgccgg actcttggaa 3120
gctcaaggag attttcgcca caggcatcgt gctggggtcc tacctcgctc tgatgactgt 3180
catcttcttc tgggccatgc acaagaccga cttcttcagc gataagttcg gcgtccgctc 3240
catcagggac agcgagcatg agatgatgtc ggcgctctac ctgcaggtct caattgtttc 3300
ccaggccctc atcttcgtga cccggtcacg ctcctggagc ttcgttgaga ggccaggcct 3360
gctcctggtg acggccttcc tcctggcgca gctggtcgct acattcctcg ccgtttacgc 3420
gaactggggc ttcgcgcgca ttaaggggat cggctggggg tgggctggcg tcgtttggct 3480
gtactccatc gtcttctact tcccgctcga cctgattaag ttcttcatca ggttcgtgct 3540
cagcggccgg gcgtgggata acctcctgga gaataagacg gccttcacca cgaagaagga 3600
ctacggcagg gaggagcgcg aggctcagct cgctactgct cagcgcaccc tccacggcct 3660
gcagccacca gaggctgcta cgtcgacact gttccatgac aagaactcat acagggagct 3720
ctccgagatt gctgagcagg ccaagcgcag ggcggagatc gctaggctca gggagctgaa 3780
caccctcaag gggcacgtcg agtccgtggt caagctgaag ggcctcgaca ttgatactat 3840
ccagcagaat tacaccgtgt agaactagac cgcgcccgcc ggccgccccc cgccggctag 3900
ctagctagct agctagctcc tgcgtgagct agtagctagt gccatgcgtc gtctctgtcg 3960
ttcggttttg cttcgggtca ccgtaccctt tgcttgcttg gtttcttctt tccttttttc 4020
cttttttttt tcttcttttc cccggccatg gttcctttgc tttcagcagt tctctgctgg 4080
atgtgatgta tccattgttg caagcatggc cttgcattgg ctacctctat acctgctaca 4140
aactactgca acgcctatat atacttgggg tgaggaacat gtgaatgcaa gctccggcta 4200
tcatatacat gtaatatgga tacaaactat atatataaat ccgccgaggc gccgactaat 4260
actatacgac gacaccgtgt taagttaata tataactggt gctttttatt tatatatctg 4320
tctcatcata tatatatgct aattaatgga tgtgtgtcct cttcacttca attccttctt 4380
tcctttccta tgctttgaga tc 4402
<210> 60
<211> 3841
<212> DNA
<213> 人工的
<220>
<223> 盒_T10401_RAB17_del ZmAHA5_W885L
<400> 60
aaatgttatg cagttcgctc tggacttttc tgctgcgcct acacttgggt gtactgggcc 60
taaattcagc ctgaccgacc gcctgcattg aataatggat gagcaccggt aaaatccgcg 120
tacccaactt tcgagaagaa ccgagacgtg gcgggccggg ccaccgacgc acggcaccag 180
cgactgcaca cgtcccgccg gcgtacgtgt acgtgctgtt ccctcactgg ccgcccaatc 240
cactcatgca tgcccacgta cacccctgcc gtggcgcgcc cagatcctaa tcctttcgcc 300
gttctgcact tctgctgcct ataaatggcg gcatcgaccg tcacctgctt caccaccggc 360
gagccacatc gagaacacga tcgagcacac aagcacgaag actcgtttag gagaaaccac 420
aaaccaccaa gccgtgcaag catctctcca ccatgggccc actgcagagg cgcccaacag 480
cgatgggggg cctggaggag atcaagaacg aggccgtcga tctggagaac atccctattg 540
aggaggtgtt cgagcagctg aagtgcaccc gggagggcct cagctcctcg gagggccagc 600
agcgcctgga gatcttcggc ccaaaccggc tggaggagaa gaaggagagc aagatcctca 660
agttcctggg gttcatgtgg aacccgctgt cctgggtcat ggagatggct gccgtgatgg 720
cgattgctct ggctaatggc gggggcaagc caccggactg ggaggatttc gtggggatta 780
tcgtcctgct cgtcatcaac agcactatct ccttcattga ggagaacaac gctggcaacg 840
ccgctgctgc gctgatggcg aacctcgctc caaagactaa ggtcctccgc gacggccgct 900
ggggggagca ggaggctgcg attctcgtgc ccggggacat cgttagcatt aagctcgggg 960
atatcgtccc tgctgatgcc cggctgctgg agggggaccc cctgaaggtc gaccagagcg 1020
ctctcaccgg cgagtcgctc ccagtcacta agggccctgg ggacgaggtg ttcagcggct 1080
ctacgtgcaa gcagggcgag atcgaggcgg tcgtgatcgc taccggcgtg cacaccttct 1140
tcgggaaggc cgcccatctc gtggattcga caaaccaggt cgggcacttc cagcaggtgc 1200
tgacagccat cgggaacttc tgcatctgct cgattgccgt tgggatcgtg gttgagatta 1260
ttgtgatgtt cccgattcag caccggcgct accgctccgg gatcgagaac ctcctggtcc 1320
tcctcattgg ggggattccg atcgctatgc cgaccgtcct gagcgtcact atggctatcg 1380
ggtctcacaa gctctcacag cagggggcga ttaccaagcg gatgacagcg attgaggaga 1440
tggcggggat ggacgtgctg tgctcagaca agaccggcac tctgacgctc aacaagctct 1500
ccgtggacaa gaacctcgtc gaggtgttct gcaagggggt ggacaaggat catgtcctgc 1560
tgctggcggc cagggcctcc cggacagaga accaggacgc cattgacgcc gctatggtgg 1620
ggatgctggc cgaccctaag gaggcgaggg ccggcatccg cgagattcac ttcctgccat 1680
tcaatcccgt tgacaagcgc actgcgctga cgtacattga tgcggacggg cactggcaca 1740
gggtgtccaa gggggctcca gagcagatcc tcgatctgtg ccactgcaag gaggatctgc 1800
ggcgcaaggt gcatgggatc attgacaagt acgcggagag ggggctccgg agcctggcgg 1860
tggccaggca ggaggtgccg gagcgcaaca aggagtcgcc ggggggccct tggcagttcg 1920
tcgggctgct ccctctgttc gatcccccga ggcacgacag cgcggagact atccgcaagg 1980
ccctcgttct gggggtgaat gtcaagatga ttaccgggga ccagctggcc attgggaagg 2040
agacaggccg gcgcctcggg atgggcacga acatgtaccc gtcatccgcc ctgctggggc 2100
agaataagga cgccactctg gaggccctgc cggtcgatga gctcatcgag aaggctgacg 2160
ggttcgcggg cgtcttcccc gagcataagt acgagatcgt gaagcggctg caggagaaga 2220
agcatattgt ggggatgact ggcgacgggg tgaatgatgc tcccgccctg aagaaggctg 2280
acatcggcat tgccgtcgcg gacgccacag atgctgcccg ctcggcgtcc gatatcgttc 2340
tcacggagcc tgggctgagc gttattatct cggcggttct gacaagcagg tgcatcttcc 2400
agcgcatgaa gaactacacc atctacgctg tgtcgatcac tatcaggatc gtgctgggct 2460
tcatgctgat tgcgctcatc tggcagtacg acttctcccc tttcatggtc ctgatcattg 2520
ctatcctcaa cgacggcaca atcatgacta tctcgaagga ccgcgtgaag ccgagcccac 2580
tccccgactc ttggaagctc aaggagattt tcgccactgg gatcgttctc gggtcttacc 2640
tcgctctgat gaccgtgatt ttcttctggg ctatgcacaa gaccgacttc ttcagcgaca 2700
agttcggggt tcgctcgatt cgcgactcgg agcacgagat gatgtctgcg ctgtacctcc 2760
aggtttcaat tgtttcacag gcgctcattt tcgtgactag gtcacggtct tggtccttcg 2820
tcgagaggcc tggcctgctc ctggtcactg ctttcctgct cgcccagctg gtggccactt 2880
tcctcgctgt ttacgctaat tgggggttcg ctaggatcaa ggggatcggc tgggggtggg 2940
cgggcgtggt ctggctctac tccatcgttt tctacttccc actcgacctc atcaagttct 3000
tcattcggtt cgtgctgtca gggagggcgt gggacaacct cctggagaac aagaccgcgt 3060
tcaccactaa gaaggactac ggccgcgagg agagggaggc gcagctggct acagcgcagc 3120
gcaccctcca cgggctccag cccccagagg ctgcgacatc tacactcttc cacgacaaga 3180
attcttaccg cgagctgtca gagattgccg agcaggctaa gcgcagggcg gagattgcta 3240
ggctgaggga gctcaacaca ctgaagggcc acgtggagtc agtggtcaag ctgaagggcc 3300
tggacatcga tacaattcag cagaactaca ccgtttagaa cgcggcgcct atacgtggct 3360
gtgctgtgct gtctggcgcg tcaaagccgt actcttcagc gttccataga taataagata 3420
aacccatgaa taagtgtccc taccctttga tcatgtgaca gggacaggga cagtttcctg 3480
tacttgtggg gtgaatttgt acgtgtgata cggtaacttg gtccgtgtac tgtttatgct 3540
gctacgtttc cttcctaaaa gtacgtatat atatacagca cttgcgagtg gctttacttg 3600
tgtgcactgc ctaaaagtgt cgatattacc tccagcggta aataaatgac ccttgtaaga 3660
aacttacaca gcatgtctta ctaattaaca gccgttatta tgttcggcgc agtactcaca 3720
ctggaactta cgaacaagaa ttgctctatc aatcaccatc tactttagat atatcttctg 3780
aaactttttt caaatccaaa agttcagtat cttcctacaa attagtgaat taggaatcca 3840
a 3841
<210> 61
<211> 4328
<212> DNA
<213> 人工的
<220>
<223> 盒_T10584_MYB60 ZmAHA5_W885L
<400> 61
ctccgtcgct agcgttgtaa tttttatcag cctctctcca tctgattgga tgagctgctt 60
ctcctagcta gcacgttgtc aaccattggg gacgcccttt aggagtgaaa gtgatgcagt 120
tactctactc ccatccggtc caaatcccaa cctaagggat atatgacgaa gagttttcaa 180
tttcagtata atgtgtatag caagtgattt gattttcctg cagattcttg agagagaagg 240
gccaaacctg catatgtgcc aggtgcgcac tcacagtcac acacacggtc ctcatcaatg 300
cccaagaaat ctggagagac acagacaaac gcaagcaatt actccattca tgagcgccac 360
agcagaaaaa ggagtcctcc agaaccggcc gtgttcttgg cttgcacaca cacacacaca 420
cacgcgaaag cagcgcatgc aacaaagcaa agatcacaca cggccggagc aagagagacg 480
cccatattca tgtgatcacc tggctgactt ttgcattgga aagcaacagt gcgagatcaa 540
aagcgaacgc agcacacagc acagcacagc acagggatcg agttgctcta ctccgtatat 600
gaggtcatgc agtatataag cactcacatg cagctatacc ttagctaggg cacctgtgca 660
aggggacagg taggagataa agcaatgtct gctggctaga ataaaaggag gccgatatcg 720
tatcagcaga gggttccagg ggagaaggcc gttctgcagg gccgccgtaa aaggctgtgt 780
aggaagcagc cacagcttct catatcttct tgttcttcga ctacttgcct agctactata 840
tctactagac tagccctagg ctgcttgcta gctagtaggt gtatcgatcg tccgaggtag 900
gaggagacag caggaagcag gctctccacc atgggcccac tgcagaggcg cccaacagcg 960
atggggggcc tggaggagat caagaacgag gccgtcgatc tggagaacat ccctattgag 1020
gaggtgttcg agcagctgaa gtgcacccgg gagggcctca gctcctcgga gggccagcag 1080
cgcctggaga tcttcggccc aaaccggctg gaggagaaga aggagagcaa gatcctcaag 1140
ttcctggggt tcatgtggaa cccgctgtcc tgggtcatgg agatggctgc cgtgatggcg 1200
attgctctgg ctaatggcgg gggcaagcca ccggactggg aggatttcgt ggggattatc 1260
gtcctgctcg tcatcaacag cactatctcc ttcattgagg agaacaacgc tggcaacgcc 1320
gctgctgcgc tgatggcgaa cctcgctcca aagactaagg tcctccgcga cggccgctgg 1380
ggggagcagg aggctgcgat tctcgtgccc ggggacatcg ttagcattaa gctcggggat 1440
atcgtccctg ctgatgcccg gctgctggag ggggaccccc tgaaggtcga ccagagcgct 1500
ctcaccggcg agtcgctccc agtcactaag ggccctgggg acgaggtgtt cagcggctct 1560
acgtgcaagc agggcgagat cgaggcggtc gtgatcgcta ccggcgtgca caccttcttc 1620
gggaaggccg cccatctcgt ggattcgaca aaccaggtcg ggcacttcca gcaggtgctg 1680
acagccatcg ggaacttctg catctgctcg attgccgttg ggatcgtggt tgagattatt 1740
gtgatgttcc cgattcagca ccggcgctac cgctccggga tcgagaacct cctggtcctc 1800
ctcattgggg ggattccgat cgctatgccg accgtcctga gcgtcactat ggctatcggg 1860
tctcacaagc tctcacagca gggggcgatt accaagcgga tgacagcgat tgaggagatg 1920
gcggggatgg acgtgctgtg ctcagacaag accggcactc tgacgctcaa caagctctcc 1980
gtggacaaga acctcgtcga ggtgttctgc aagggggtgg acaaggatca tgtcctgctg 2040
ctggcggcca gggcctcccg gacagagaac caggacgcca ttgacgccgc tatggtgggg 2100
atgctggccg accctaagga ggcgagggcc ggcatccgcg agattcactt cctgccattc 2160
aatcccgttg acaagcgcac tgcgctgacg tacattgatg cggacgggca ctggcacagg 2220
gtgtccaagg gggctccaga gcagatcctc gatctgtgcc actgcaagga ggatctgcgg 2280
cgcaaggtgc atgggatcat tgacaagtac gcggagaggg ggctccggag cctggcggtg 2340
gccaggcagg aggtgccgga gcgcaacaag gagtcgccgg ggggcccttg gcagttcgtc 2400
gggctgctcc ctctgttcga tcccccgagg cacgacagcg cggagactat ccgcaaggcc 2460
ctcgttctgg gggtgaatgt caagatgatt accggggacc agctggccat tgggaaggag 2520
acaggccggc gcctcgggat gggcacgaac atgtacccgt catccgccct gctggggcag 2580
aataaggacg ccactctgga ggccctgccg gtcgatgagc tcatcgagaa ggctgacggg 2640
ttcgcgggcg tcttccccga gcataagtac gagatcgtga agcggctgca ggagaagaag 2700
catattgtgg ggatgactgg cgacggggtg aatgatgctc ccgccctgaa gaaggctgac 2760
atcggcattg ccgtcgcgga cgccacagat gctgcccgct cggcgtccga tatcgttctc 2820
acggagcctg ggctgagcgt tattatctcg gcggttctga caagcaggtg catcttccag 2880
cgcatgaaga actacaccat ctacgctgtg tcgatcacta tcaggatcgt gctgggcttc 2940
atgctgattg cgctcatctg gcagtacgac ttctcccctt tcatggtcct gatcattgct 3000
atcctcaacg acggcacaat catgactatc tcgaaggacc gcgtgaagcc gagcccactc 3060
cccgactctt ggaagctcaa ggagattttc gccactggga tcgttctcgg gtcttacctc 3120
gctctgatga ccgtgatttt cttctgggct atgcacaaga ccgacttctt cagcgacaag 3180
ttcggggttc gctcgattcg cgactcggag cacgagatga tgtctgcgct gtacctccag 3240
gtttcaattg tttcacaggc gctcattttc gtgactaggt cacggtcttg gtccttcgtc 3300
gagaggcctg gcctgctcct ggtcactgct ttcctgctcg cccagctggt ggccactttc 3360
ctcgctgttt acgctaattg ggggttcgct aggatcaagg ggatcggctg ggggtgggcg 3420
ggcgtggtct ggctctactc catcgttttc tacttcccac tcgacctcat caagttcttc 3480
attcggttcg tgctgtcagg gagggcgtgg gacaacctcc tggagaacaa gaccgcgttc 3540
accactaaga aggactacgg ccgcgaggag agggaggcgc agctggctac agcgcagcgc 3600
accctccacg ggctccagcc cccagaggct gcgacatcta cactcttcca cgacaagaat 3660
tcttaccgcg agctgtcaga gattgccgag caggctaagc gcagggcgga gattgctagg 3720
ctgagggagc tcaacacact gaagggccac gtggagtcag tggtcaagct gaagggcctg 3780
gacatcgata caattcagca gaactacacc gtttagaacc taaatgctct taactgagct 3840
aattatgtaa tgcacataca catatttaca tagatatgca tatttatata tagcatgtat 3900
attgtactac atgcattgct tcttaataca tgtagtaaag atatatgcaa aaatagtcga 3960
aagatttgtt tacatataaa atcaccaata tttattgtta ttgtattttc atgaataaag 4020
taataagatt atttgtctaa tattttgatt tactagtact agaaatgaaa aggaatatgc 4080
acaatttcag cattatagtt tggtaggcaa aatggagtga gaatagagtt tcatagtata 4140
tactaaggtt cttaattgtg caaatagttg atacaagtca catgggccaa gtttgtaaat 4200
cttaaatcga aatatgcctt cttctttttt tgcatgaaaa tgctagtaat ttataagtgt 4260
gtttttcaat aagagatgct aaataccaaa attaacctag ttttcagtga gcgcttgcat 4320
tattgtgg 4328
<210> 62
<211> 2796
<212> DNA
<213> 拟南芥
<220>
<221> misc_feature
<223> AtAHA5W857L
<400> 62
atggaggagg ttttcgagga gctgaagtgc accaagcagg ggctgaccgc taatgaggcg 60
tcccacaggc tggatgtttt cgggccaaac aagctggagg agaagaagga gtctaagctc 120
ctgaagttcc tgggcttcat gtggaacccc ctctcatggg ttatggaggt ggctgctctg 180
atggctatcg ctctcgccaa tggcgggggc aggccgcccg actggcagga tttcgtcggc 240
attgtttgcc tcctgctcat caactctacg atttcattca tcgaggagaa caatgctggc 300
aatgctgccg cggctctgat ggccgggctc gcgccaaaga caaaggtgct cagggacaac 360
cagtggtctg agcaggaggc gtcaatcctg gtgccaggcg atgtcatctc cattaagctc 420
ggggacatca ttccagctga tgctaggctg ctcgacggcg atccactgaa gatcgaccag 480
tccagcctca cgggcgagtc gattcccgtc acaaagaacc catctgatga ggttttcagc 540
ggctcgattt gcaagcaggg ggagatcgag gccatcgtca ttgcgactgg cgttcacacc 600
ttcttcggga aggccgcgca tctggtggac aacactaatc agatcggcca cttccagaag 660
gtcctgacct caattgggaa cttctgcatt tgctccatcg ccctcggcat cattgttgag 720
ctgctcgtga tgtacccaat ccagaggagg cgctaccgcg acggcattga taatctgctc 780
gtgctgctca tcgggggcat ccctattgcg atgccgtccg tgctgagcgt cacgatggct 840
acaggctccc accgcctctt ccagcagggg gctattacga agcgcatgac agctatcgag 900
gagatggccg ggatggacgt gctctgctgc gataagacag gcactctgac cctcaacaag 960
ctgaccgtcg acaagaatct cgtcgaggtt ttcgcgaagg gggtgggcaa ggagcatgtc 1020
ttcctgctcg ctgcccgcgc ctcccgcatc gagaaccagg acgccattga tgctgctatc 1080
gtcggcatgc tggccgaccc aaaggaggcc agggcgggcg ttcgggaggt gcacttcttc 1140
cctttcaacc cggtggacaa gcgcactgcg ctcacctacg tcgacagcga tggcaattgg 1200
catagggctt cgaagggggc cccagagcag atcctgaacc tctgcaattg caaggaggac 1260
gtgcgcagga aggtccacgg cgttatcgat aagttcgctg agagggggct gaggtcgctc 1320
gctgttgccc ggcaggaggt gctggagaag aagaaggacg ctccaggggg cccgtggcag 1380
ctggtcggcc tgctccctct cttcgaccca cctcgccatg atagcgctga gacgatccgg 1440
cgcgctctga acctcggcgt gaatgtcaag atgattacag gggaccagct ggccatcggc 1500
aaggagacgg ggaggaggct cgggatgggc acaaacatgt acccgtcgtc tgctctgctc 1560
ggccaggtca aggactcatc cctgggggcg ctccctgttg acgagctcat cgagaaggcg 1620
gatggcttcg ctggggtttt cccggagcac aagtacgaga tcgtgcatcg cctgcagcag 1680
aggaaccaca tctgcggcat gacgggggac ggcgtgaatg atgcgcctgc tctcaagaag 1740
gccgacatcg gcattgccgt ggtcgacgct acggatgctg cgaggggggc gagcgatatc 1800
gtcctgacag agccgggcct ctcagtcatc atttccgccg ttctgacctc ccgggcgatt 1860
ttccagcgca tgaagaacta cactatctac gccgttagca tcaccattcg catcgtgttc 1920
ggcttcatgt tcattgcgct catctggcag ttcgacttct cgccgttcat ggtgctgatc 1980
attgccatcc tcaatgacgg cacgattatg acaatctcta aggataggat gaagccgtcc 2040
ccacagccag acagctggaa gctgagggat atcttctcca ctggcgttgt gctggggggc 2100
taccaggctc tcatgaccgt cgtgttcttc tgggtcatga aggacagcga cttcttctcc 2160
aactacttcg gcgtcaggcc tctgagccag aggccagagc agatgatggc tgctctgtac 2220
ctccaggtgt cgatcatttc tcaggccctc atcttcgtca cccggtctcg ctcatggtcc 2280
tacgctgagt gcccaggcct gctcctgctc ggggctttcg tgattgccca gctggtcgcg 2340
accttcatcg ctgtgtacgc caactggtca ttcgcgagga tcgagggggc tggctggggg 2400
tgggctggcg tcatttggct gtactccttc ctcacctaca tcccactcga cctgctcaag 2460
ttcggcattc gctacgtgct gagcgggaag gcctggctca acctcctgga gaataagact 2520
gcgttcacca cgaagaagga ctacggcaag gaggagcgcg aggctcagct ggcggctgcc 2580
cagaggaccc tgcacggcct ccagccggcg gagaagaaca atatcttcaa cgagaagaat 2640
agctactcgg agctctcgca gatcgctgag caggctaagc gcagggccga ggtggtcagg 2700
ctgagggaga tcaacacgct caagggccac gtggagtctg ttgtgaagct gaagggcctc 2760
gacattgata ctatccagca gcattacacc gtctag 2796
<210> 63
<211> 4326
<212> DNA
<213> 人工的
<220>
<223> 盒_T02780_CsVMV AtAHA5_W857L
<400> 63
aaggtaatta tccaagatgt agcatcaaga atccaatgtt tacgggaaaa actatggaag 60
tattatgtga gctcagcaag aagcagatca atatgcggca catatgcaac ctatgttcaa 120
aaatgaagaa tgtacagata caagatccta tactgccaga atacgaagaa gaatacgtag 180
aaattgaaaa agaagaacca ggcgaagaaa agaatcttga agacgtaagc actgacgaca 240
acaatgaaaa gaagaagata aggtcggtga ttgtgaaaga gacatagagg acacatgtaa 300
ggtggaaaat gtaagggcgg aaagtaacct tatcacaaag gaatcttatc ccccactact 360
tatcctttta tatttttccg tgtcattttt gcccttgagt tttcctatat aaggaaccaa 420
gttcggcatt tgtgaaaaca agaaaaaatt tggtgtaagc tattttcttt gaagtactga 480
ggatacaact tcagagaaat ttgtccctcc cccctccccc tccgccgccg ccgcgccggt 540
aaccaccccg cccctctcct ctttctttct ccgttttttt tttccgtctc ggtctcgatc 600
tttggccttg gtagtttggg tgggcgagag gcggcttcgt gcgcgcccag atcggtgcgc 660
gggaggggcg ggatctcgcg gctggggctc tcgccggcgt ggatccggcc cggatctcgc 720
ggggaatggg gctctcggat gtagatctgc gatccgccgt tgttggggga gatgatgggg 780
ggtttaaaat ttccgccatg ctaaacaaga tcaggaagag gggaaaaggg cactatggtt 840
tatattttta tatatttctg ctgcttcgtc aggcttagat gtgctagatc tttctttctt 900
ctttttgtgg gtagaatttg aatccctcag cattgttcat cggtagtttt tcttttcatg 960
atttgtgaca aatgcagcct cgtgcggagc ttttttgtag gtagacgata tctccaccat 1020
ggaggaggtt ttcgaggagc tgaagtgcac caagcagggg ctgaccgcta atgaggcgtc 1080
ccacaggctg gatgttttcg ggccaaacaa gctggaggag aagaaggagt ctaagctcct 1140
gaagttcctg ggcttcatgt ggaaccccct ctcatgggtt atggaggtgg ctgctctgat 1200
ggctatcgct ctcgccaatg gcgggggcag gccgcccgac tggcaggatt tcgtcggcat 1260
tgtttgcctc ctgctcatca actctacgat ttcattcatc gaggagaaca atgctggcaa 1320
tgctgccgcg gctctgatgg ccgggctcgc gccaaagaca aaggtgctca gggacaacca 1380
gtggtctgag caggaggcgt caatcctggt gccaggcgat gtcatctcca ttaagctcgg 1440
ggacatcatt ccagctgatg ctaggctgct cgacggcgat ccactgaaga tcgaccagtc 1500
cagcctcacg ggcgagtcga ttcccgtcac aaagaaccca tctgatgagg ttttcagcgg 1560
ctcgatttgc aagcaggggg agatcgaggc catcgtcatt gcgactggcg ttcacacctt 1620
cttcgggaag gccgcgcatc tggtggacaa cactaatcag atcggccact tccagaaggt 1680
cctgacctca attgggaact tctgcatttg ctccatcgcc ctcggcatca ttgttgagct 1740
gctcgtgatg tacccaatcc agaggaggcg ctaccgcgac ggcattgata atctgctcgt 1800
gctgctcatc gggggcatcc ctattgcgat gccgtccgtg ctgagcgtca cgatggctac 1860
aggctcccac cgcctcttcc agcagggggc tattacgaag cgcatgacag ctatcgagga 1920
gatggccggg atggacgtgc tctgctgcga taagacaggc actctgaccc tcaacaagct 1980
gaccgtcgac aagaatctcg tcgaggtttt cgcgaagggg gtgggcaagg agcatgtctt 2040
cctgctcgct gcccgcgcct cccgcatcga gaaccaggac gccattgatg ctgctatcgt 2100
cggcatgctg gccgacccaa aggaggccag ggcgggcgtt cgggaggtgc acttcttccc 2160
tttcaacccg gtggacaagc gcactgcgct cacctacgtc gacagcgatg gcaattggca 2220
tagggcttcg aagggggccc cagagcagat cctgaacctc tgcaattgca aggaggacgt 2280
gcgcaggaag gtccacggcg ttatcgataa gttcgctgag agggggctga ggtcgctcgc 2340
tgttgcccgg caggaggtgc tggagaagaa gaaggacgct ccagggggcc cgtggcagct 2400
ggtcggcctg ctccctctct tcgacccacc tcgccatgat agcgctgaga cgatccggcg 2460
cgctctgaac ctcggcgtga atgtcaagat gattacaggg gaccagctgg ccatcggcaa 2520
ggagacgggg aggaggctcg ggatgggcac aaacatgtac ccgtcgtctg ctctgctcgg 2580
ccaggtcaag gactcatccc tgggggcgct ccctgttgac gagctcatcg agaaggcgga 2640
tggcttcgct ggggttttcc cggagcacaa gtacgagatc gtgcatcgcc tgcagcagag 2700
gaaccacatc tgcggcatga cgggggacgg cgtgaatgat gcgcctgctc tcaagaaggc 2760
cgacatcggc attgccgtgg tcgacgctac ggatgctgcg aggggggcga gcgatatcgt 2820
cctgacagag ccgggcctct cagtcatcat ttccgccgtt ctgacctccc gggcgatttt 2880
ccagcgcatg aagaactaca ctatctacgc cgttagcatc accattcgca tcgtgttcgg 2940
cttcatgttc attgcgctca tctggcagtt cgacttctcg ccgttcatgg tgctgatcat 3000
tgccatcctc aatgacggca cgattatgac aatctctaag gataggatga agccgtcccc 3060
acagccagac agctggaagc tgagggatat cttctccact ggcgttgtgc tggggggcta 3120
ccaggctctc atgaccgtcg tgttcttctg ggtcatgaag gacagcgact tcttctccaa 3180
ctacttcggc gtcaggcctc tgagccagag gccagagcag atgatggctg ctctgtacct 3240
ccaggtgtcg atcatttctc aggccctcat cttcgtcacc cggtctcgct catggtccta 3300
cgctgagtgc ccaggcctgc tcctgctcgg ggctttcgtg attgcccagc tggtcgcgac 3360
cttcatcgct gtgtacgcca actggtcatt cgcgaggatc gagggggctg gctgggggtg 3420
ggctggcgtc atttggctgt actccttcct cacctacatc ccactcgacc tgctcaagtt 3480
cggcattcgc tacgtgctga gcgggaaggc ctggctcaac ctcctggaga ataagactgc 3540
gttcaccacg aagaaggact acggcaagga ggagcgcgag gctcagctgg cggctgccca 3600
gaggaccctg cacggcctcc agccggcgga gaagaacaat atcttcaacg agaagaatag 3660
ctactcggag ctctcgcaga tcgctgagca ggctaagcgc agggccgagg tggtcaggct 3720
gagggagatc aacacgctca agggccacgt ggagtctgtt gtgaagctga agggcctcga 3780
cattgatact atccagcagc attacaccgt ctagaaccta aatgctctta actgagctaa 3840
ttatgtaatg cacatacaca tatttacata gatatgcata tttatatata gcatgtatat 3900
tgtactacat gcattgcttc ttaatacatg tagtaaagat atatgcaaaa atagtcgaaa 3960
gatttgttta catataaaat caccaatatt tattgttatt gtattttcat gaataaagta 4020
ataagattat ttgtctaata ttttgattta ctagtactag aaatgaaaag gaatatgcac 4080
aatttcagca ttatagtttg gtaggcaaaa tggagtgaga atagagtttc atagtatata 4140
ctaaggttct taattgtgca aatagttgat acaagtcaca tgggccaagt ttgtaaatct 4200
taaatcgaaa tatgccttct tctttttttg catgaaaatg ctagtaattt ataagtgtgt 4260
ttttcaataa gagatgctaa ataccaaaat taacctagtt ttcagtgagc gcttgcatta 4320
ttgtgg 4326
<210> 64
<211> 4312
<212> DNA
<213> 人工的
<220>
<223> 盒_T02783_rbcS_Mut_al AtAHA5_W857L
<400> 64
tccctttaat ctggcgctag atctgcatcc gcggcttgca aagataaatg gcacatttag 60
tgtgttattt tgcaatacct ttcatagtag atatccttaa atgcagtttt aggcatgttt 120
gggtaattaa ataacatttt taggaggagt tttagattta cctttctttc gtgatgactg 180
atgacagacg tggggaattc aaatgcaact ctagcgaaag ttcatatatt tttcataaat 240
agctgaggct ggggtaatta ttttttttgt agaaaaatag aataggtgga atggttgggg 300
aaggcgtagg cgctcgtgga cgacgcccga taaaagacaa gaggcggaat tgccatgaat 360
tcgaggtagc taagtaaggc gcatatatat gccaaaaaat tctactgtca ctttccaatt 420
tcaatgcgct gccaaacaag ccatcctgga aactgacttg aattcagccc aattctgtag 480
atccaaacag ggccggcgtc agtgcctcag gtgagagagc agcagacgat tcaaagagcc 540
aaaactgcaa gcagacgcag ccgaagccga agccgaagcc caagcccaaa actgttttgt 600
ctttgcccag aaccgcgacg agcctaaact gcgcttcctc ctatctacaa gtccctggca 660
catcacgcat agtccaacct aggcgcgcag gcgataaggc gcgccacggg gacgcgacat 720
gtggtggcgg acgcgatcag gatagggcca ggctggccgg gcgcggccac gggagaacgg 780
tggccactcg tcccacatcc gcttcgtcct gtcctgtact gcgtcctgcc cccaacgaga 840
gccggagccg gccatcccgt cgcacactct ccccctctat atatgccgtc ggtgtggggg 900
agcctactac aggacgaccc aagcaagcaa gcaagcagcg agtacataca tactaggcag 960
ccaggcagtc tccaccatgg aggaggtttt cgaggagctg aagtgcacca agcaggggct 1020
gaccgctaat gaggcgtccc acaggctgga tgttttcggg ccaaacaagc tggaggagaa 1080
gaaggagtct aagctcctga agttcctggg cttcatgtgg aaccccctct catgggttat 1140
ggaggtggct gctctgatgg ctatcgctct cgccaatggc gggggcaggc cgcccgactg 1200
gcaggatttc gtcggcattg tttgcctcct gctcatcaac tctacgattt cattcatcga 1260
ggagaacaat gctggcaatg ctgccgcggc tctgatggcc gggctcgcgc caaagacaaa 1320
ggtgctcagg gacaaccagt ggtctgagca ggaggcgtca atcctggtgc caggcgatgt 1380
catctccatt aagctcgggg acatcattcc agctgatgct aggctgctcg acggcgatcc 1440
actgaagatc gaccagtcca gcctcacggg cgagtcgatt cccgtcacaa agaacccatc 1500
tgatgaggtt ttcagcggct cgatttgcaa gcagggggag atcgaggcca tcgtcattgc 1560
gactggcgtt cacaccttct tcgggaaggc cgcgcatctg gtggacaaca ctaatcagat 1620
cggccacttc cagaaggtcc tgacctcaat tgggaacttc tgcatttgct ccatcgccct 1680
cggcatcatt gttgagctgc tcgtgatgta cccaatccag aggaggcgct accgcgacgg 1740
cattgataat ctgctcgtgc tgctcatcgg gggcatccct attgcgatgc cgtccgtgct 1800
gagcgtcacg atggctacag gctcccaccg cctcttccag cagggggcta ttacgaagcg 1860
catgacagct atcgaggaga tggccgggat ggacgtgctc tgctgcgata agacaggcac 1920
tctgaccctc aacaagctga ccgtcgacaa gaatctcgtc gaggttttcg cgaagggggt 1980
gggcaaggag catgtcttcc tgctcgctgc ccgcgcctcc cgcatcgaga accaggacgc 2040
cattgatgct gctatcgtcg gcatgctggc cgacccaaag gaggccaggg cgggcgttcg 2100
ggaggtgcac ttcttccctt tcaacccggt ggacaagcgc actgcgctca cctacgtcga 2160
cagcgatggc aattggcata gggcttcgaa gggggcccca gagcagatcc tgaacctctg 2220
caattgcaag gaggacgtgc gcaggaaggt ccacggcgtt atcgataagt tcgctgagag 2280
ggggctgagg tcgctcgctg ttgcccggca ggaggtgctg gagaagaaga aggacgctcc 2340
agggggcccg tggcagctgg tcggcctgct ccctctcttc gacccacctc gccatgatag 2400
cgctgagacg atccggcgcg ctctgaacct cggcgtgaat gtcaagatga ttacagggga 2460
ccagctggcc atcggcaagg agacggggag gaggctcggg atgggcacaa acatgtaccc 2520
gtcgtctgct ctgctcggcc aggtcaagga ctcatccctg ggggcgctcc ctgttgacga 2580
gctcatcgag aaggcggatg gcttcgctgg ggttttcccg gagcacaagt acgagatcgt 2640
gcatcgcctg cagcagagga accacatctg cggcatgacg ggggacggcg tgaatgatgc 2700
gcctgctctc aagaaggccg acatcggcat tgccgtggtc gacgctacgg atgctgcgag 2760
gggggcgagc gatatcgtcc tgacagagcc gggcctctca gtcatcattt ccgccgttct 2820
gacctcccgg gcgattttcc agcgcatgaa gaactacact atctacgccg ttagcatcac 2880
cattcgcatc gtgttcggct tcatgttcat tgcgctcatc tggcagttcg acttctcgcc 2940
gttcatggtg ctgatcattg ccatcctcaa tgacggcacg attatgacaa tctctaagga 3000
taggatgaag ccgtccccac agccagacag ctggaagctg agggatatct tctccactgg 3060
cgttgtgctg gggggctacc aggctctcat gaccgtcgtg ttcttctggg tcatgaagga 3120
cagcgacttc ttctccaact acttcggcgt caggcctctg agccagaggc cagagcagat 3180
gatggctgct ctgtacctcc aggtgtcgat catttctcag gccctcatct tcgtcacccg 3240
gtctcgctca tggtcctacg ctgagtgccc aggcctgctc ctgctcgggg ctttcgtgat 3300
tgcccagctg gtcgcgacct tcatcgctgt gtacgccaac tggtcattcg cgaggatcga 3360
gggggctggc tgggggtggg ctggcgtcat ttggctgtac tccttcctca cctacatccc 3420
actcgacctg ctcaagttcg gcattcgcta cgtgctgagc gggaaggcct ggctcaacct 3480
cctggagaat aagactgcgt tcaccacgaa gaaggactac ggcaaggagg agcgcgaggc 3540
tcagctggcg gctgcccaga ggaccctgca cggcctccag ccggcggaga agaacaatat 3600
cttcaacgag aagaatagct actcggagct ctcgcagatc gctgagcagg ctaagcgcag 3660
ggccgaggtg gtcaggctga gggagatcaa cacgctcaag ggccacgtgg agtctgttgt 3720
gaagctgaag ggcctcgaca ttgatactat ccagcagcat tacaccgtct agaactagac 3780
cgcgcccgcc ggccgccccc cgccggctag ctagctagct agctagctcc tgcgtgagct 3840
agtagctagt gccatgcgtc gtctctgtcg ttcggttttg cttcgggtca ccgtaccctt 3900
tgcttgcttg gtttcttctt tccttttttc cttttttttt tcttcttttc cccggccatg 3960
gttcctttgc tttcagcagt tctctgctgg atgtgatgta tccattgttg caagcatggc 4020
cttgcattgg ctacctctat acctgctaca aactactgca acgcctatat atacttgggg 4080
tgaggaacat gtgaatgcaa gctccggcta tcatatacat gtaatatgga tacaaactat 4140
atatataaat ccgccgaggc gccgactaat actatacgac gacaccgtgt taagttaata 4200
tataactggt gctttttatt tatatatctg tctcatcata tatatatgct aattaatgga 4260
tgtgtgtcct cttcacttca attccttctt tcctttccta tgctttgaga tc 4312
<210> 65
<211> 5181
<212> DNA
<213> 人工的
<220>
<223> 盒_T10191_RAB17_del AtAHA5_W857L
<400> 65
aaatgttatg cagttcgctc tggacttttc tgctgcgcct acacttgggt gtactgggcc 60
taaattcagc ctgaccgacc gcctgcattg aataatggat gagcaccggt aaaatccgcg 120
tacccaactt tcgagaagaa ccgagacgtg gcgggccggg ccaccgacgc acggcaccag 180
cgactgcaca cgtcccgccg gcgtacgtgt acgtgctgtt ccctcactgg ccgcccaatc 240
cactcatgca tgcccacgta cacccctgcc gtggcgcgcc cagatcctaa tcctttcgcc 300
gttctgcact tctgctgcct ataaatggcg gcatcgaccg tcacctgctt caccaccggc 360
gagccacatc gagaacacga tcgagcacac aagcacgaag actcgtttag gagaaaccac 420
aaaccaccaa gccgtgcaag catctctcca ccatggagga ggttttcgag gagctgaagt 480
gcaccaagca ggggctgacc gctaatgagg cgtcccacag gctggatgtt ttcgggccaa 540
acaagctgga ggagaagaag gagtctaagc tcctgaagtt cctgggcttc atgtggaacc 600
ccctctcatg ggttatggag gtggctgctc tgatggctat cgctctcgcc aatggcgggg 660
gcaggccgcc cgactggcag gatttcgtcg gcattgtttg cctcctgctc atcaactcta 720
cgatttcatt catcgaggag aacaatgctg gcaatgctgc cgcggctctg atggccgggc 780
tcgcgccaaa gacaaaggtg ctcagggaca accagtggtc tgagcaggag gcgtcaatcc 840
tggtgccagg cgatgtcatc tccattaagc tcggggacat cattccagct gatgctaggc 900
tgctcgacgg cgatccactg aagatcgacc agtccagcct cacgggcgag tcgattcccg 960
tcacaaagaa cccatctgat gaggttttca gcggctcgat ttgcaagcag ggggagatcg 1020
aggccatcgt cattgcgact ggcgttcaca ccttcttcgg gaaggccgcg catctggtgg 1080
acaacactaa tcagatcggc cacttccaga aggtcctgac ctcaattggg aacttctgca 1140
tttgctccat cgccctcggc atcattgttg agctgctcgt gatgtaccca atccagagga 1200
ggcgctaccg cgacggcatt gataatctgc tcgtgctgct catcgggggc atccctattg 1260
cgatgccgtc cgtgctgagc gtcacgatgg ctacaggctc ccaccgcctc ttccagcagg 1320
gggctattac gaagcgcatg acagctatcg aggagatggc cgggatggac gtgctctgct 1380
gcgataagac aggcactctg accctcaaca agctgaccgt cgacaagaat ctcgtcgagg 1440
ttttcgcgaa gggggtgggc aaggagcatg tcttcctgct cgctgcccgc gcctcccgca 1500
tcgagaacca ggacgccatt gatgctgcta tcgtcggcat gctggccgac ccaaaggagg 1560
ccagggcggg cgttcgggag gtgcacttct tccctttcaa cccggtggac aagcgcactg 1620
cgctcaccta cgtcgacagc gatggcaatt ggcatagggc ttcgaagggg gccccagagc 1680
agatcctgaa cctctgcaat tgcaaggagg acgtgcgcag gaaggtccac ggcgttatcg 1740
ataagttcgc tgagaggggg ctgaggtcgc tcgctgttgc ccggcaggag gtgctggaga 1800
agaagaagga cgctccaggg ggcccgtggc agctggtcgg cctgctccct ctcttcgacc 1860
cacctcgcca tgatagcgct gagacgatcc ggcgcgctct gaacctcggc gtgaatgtca 1920
agatgattac aggggaccag ctggccatcg gcaaggagac ggggaggagg ctcgggatgg 1980
gcacaaacat gtacccgtcg tctgctctgc tcggccaggt caaggactca tccctggggg 2040
cgctccctgt tgacgagctc atcgagaagg cggatggctt cgctggggtt ttcccggagc 2100
acaagtacga gatcgtgcat cgcctgcagc agaggaacca catctgcggc atgacggggg 2160
acggcgtgaa tgatgcgcct gctctcaaga aggccgacat cggcattgcc gtggtcgacg 2220
ctacggatgc tgcgaggggg gcgagcgata tcgtcctgac agagccgggc ctctcagtca 2280
tcatttccgc cgttctgacc tcccgggcga ttttccagcg catgaagaac tacactatct 2340
acgccgttag catcaccatt cgcatcgtgt tcggcttcat gttcattgcg ctcatctggc 2400
agttcgactt ctcgccgttc atggtgctga tcattgccat cctcaatgac ggcacgatta 2460
tgacaatctc taaggatagg atgaagccgt ccccacagcc agacagctgg aagctgaggg 2520
atatcttctc cactggcgtt gtgctggggg gctaccaggc tctcatgacc gtcgtgttct 2580
tctgggtcat gaaggacagc gacttcttct ccaactactt cggcgtcagg cctctgagcc 2640
agaggccaga gcagatgatg gctgctctgt acctccaggt gtcgatcatt tctcaggccc 2700
tcatcttcgt cacccggtct cgctcatggt cctacgctga gtgcccaggc ctgctcctgc 2760
tcggggcttt cgtgattgcc cagctggtcg cgaccttcat cgctgtgtac gccaactggt 2820
cattcgcgag gatcgagggg gctggctggg ggtgggctgg cgtcatttgg ctgtactcct 2880
tcctcaccta catcccactc gacctgctca agttcggcat tcgctacgtg ctgagcggga 2940
aggcctggct caacctcctg gagaataaga ctgcgttcac cacgaagaag gactacggca 3000
aggaggagcg cgaggctcag ctggcggctg cccagaggac cctgcacggc ctccagccgg 3060
cggagaagaa caatatcttc aacgagaaga atagctactc ggagctctcg cagatcgctg 3120
agcaggctaa gcgcagggcc gaggtggtca ggctgaggga gatcaacacg ctcaagggcc 3180
acgtggagtc tgttgtgaag ctgaagggcc tcgacattga tactatccag cagcattaca 3240
ccgtctagaa cgcggcgcct atacgtggct gtgctgtgct gtctggcgcg tcaaagccgt 3300
actcttcagc gttccataga taataagata aacccatgaa taagtgtccc taccctttga 3360
tcatgtgaca gggacaggga cagtttcctg tacttgtggg gtgaatttgt acgtgtgata 3420
cggtaacttg gtccgtgtac tgtttatgct gctacgtttc cttcctaaaa gtacgtatat 3480
atatacagca cttgcgagtg gctttacttg tgtgcactgc ctaaaagtgt cgatattacc 3540
tccagcggta aataaatgac ccttgtaaga aacttacaca gcatgtctta ctaattaaca 3600
gccgttatta tgttcggcgc agtactcaca ctggaactta cgaacaagaa ttgctctatc 3660
aatcaccatc tactttagat atatcttctg aaactttttt caaatccaaa agttcagtat 3720
cttcctacaa attagtgaat taggaatcca agtggttaaa cgaattcggc cggccggtac 3780
cccttaggcc tagggctaga ggacccagct ttgagtggcc gtagatttgc aaaagcaatg 3840
gctaacagac acatattctg ccaaacccca agaaggataa tcacttttct tagataaaaa 3900
agaacagacc aatatacaaa catccacact tctgcaaaca atacatcaga actaggatta 3960
cgccgattac gtggctttag cagactgtcc aaaaatctgt tttgcaaagc tccaattgct 4020
ccttgcttat ccagcttctt ttgtgttggc aaactgcgct tttccaaccg attttgttct 4080
tctcgcgctt tcttcttagc ctaaacaaac ctcaccgtgc acgcagccat ggtcctgaac 4140
cttcacctcg tccctataaa agcctagcca accttcacaa tcttatcatc acccacaaca 4200
ccgagcacca caaactagag atcctctcca ccatggctca gtcaaagcac gggctcacaa 4260
aggagatgac tatgaagtac aggatggagg gctgcgtcga tgggcacaag ttcgttatca 4320
ccggcgaggg gatcggctac ccgttcaagg gcaagcaggc gattaacctg tgcgtggtcg 4380
agggcgggcc actgcccttc gctgaggata tcctctccgc cgcgttcatg tacggcaaca 4440
gggttttcac cgagtacccg caggacattg tcgattactt caagaattcc tgcccagctg 4500
ggtacacgtg ggacaggagc ttcctcttcg aggatggcgc tgtgtgcatc tgcaacgccg 4560
acattaccgt ttcggtggag gagaattgca tgtaccacga gtctaagttc tacggggtga 4620
acttcccagc tgacggcccc gtcatgaaga agatgacgga taattgggag ccatcatgcg 4680
agaagatcat tccagtgcct aagcagggga tcctgaaggg cgacgtctcc atgtacctcc 4740
tgctcaagga tggcggcagg ctcaggtgcc agttcgacac agtctacaag gccaagagcg 4800
ttccacgcaa gatgcctgac tggcacttca tccagcataa gctgactcgg gaggaccgct 4860
cggatgcgaa gaaccagaag tggcacctga ctgagcacgc tatcgcctcg gggtccgccc 4920
tcccttgaaa cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 4980
ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 5040
acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 5100
acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 5160
cggtgtcatc tatgttacta g 5181
<210> 66
<211> 4238
<212> DNA
<213> 人工的
<220>
<223> 盒_T10586_MYB60 AtAHA5_W857L
<400> 66
ctccgtcgct agcgttgtaa tttttatcag cctctctcca tctgattgga tgagctgctt 60
ctcctagcta gcacgttgtc aaccattggg gacgcccttt aggagtgaaa gtgatgcagt 120
tactctactc ccatccggtc caaatcccaa cctaagggat atatgacgaa gagttttcaa 180
tttcagtata atgtgtatag caagtgattt gattttcctg cagattcttg agagagaagg 240
gccaaacctg catatgtgcc aggtgcgcac tcacagtcac acacacggtc ctcatcaatg 300
cccaagaaat ctggagagac acagacaaac gcaagcaatt actccattca tgagcgccac 360
agcagaaaaa ggagtcctcc agaaccggcc gtgttcttgg cttgcacaca cacacacaca 420
cacgcgaaag cagcgcatgc aacaaagcaa agatcacaca cggccggagc aagagagacg 480
cccatattca tgtgatcacc tggctgactt ttgcattgga aagcaacagt gcgagatcaa 540
aagcgaacgc agcacacagc acagcacagc acagggatcg agttgctcta ctccgtatat 600
gaggtcatgc agtatataag cactcacatg cagctatacc ttagctaggg cacctgtgca 660
aggggacagg taggagataa agcaatgtct gctggctaga ataaaaggag gccgatatcg 720
tatcagcaga gggttccagg ggagaaggcc gttctgcagg gccgccgtaa aaggctgtgt 780
aggaagcagc cacagcttct catatcttct tgttcttcga ctacttgcct agctactata 840
tctactagac tagccctagg ctgcttgcta gctagtaggt gtatcgatcg tccgaggtag 900
gaggagacag caggaagcag gctctccacc atggaggagg ttttcgagga gctgaagtgc 960
accaagcagg ggctgaccgc taatgaggcg tcccacaggc tggatgtttt cgggccaaac 1020
aagctggagg agaagaagga gtctaagctc ctgaagttcc tgggcttcat gtggaacccc 1080
ctctcatggg ttatggaggt ggctgctctg atggctatcg ctctcgccaa tggcgggggc 1140
aggccgcccg actggcagga tttcgtcggc attgtttgcc tcctgctcat caactctacg 1200
atttcattca tcgaggagaa caatgctggc aatgctgccg cggctctgat ggccgggctc 1260
gcgccaaaga caaaggtgct cagggacaac cagtggtctg agcaggaggc gtcaatcctg 1320
gtgccaggcg atgtcatctc cattaagctc ggggacatca ttccagctga tgctaggctg 1380
ctcgacggcg atccactgaa gatcgaccag tccagcctca cgggcgagtc gattcccgtc 1440
acaaagaacc catctgatga ggttttcagc ggctcgattt gcaagcaggg ggagatcgag 1500
gccatcgtca ttgcgactgg cgttcacacc ttcttcggga aggccgcgca tctggtggac 1560
aacactaatc agatcggcca cttccagaag gtcctgacct caattgggaa cttctgcatt 1620
tgctccatcg ccctcggcat cattgttgag ctgctcgtga tgtacccaat ccagaggagg 1680
cgctaccgcg acggcattga taatctgctc gtgctgctca tcgggggcat ccctattgcg 1740
atgccgtccg tgctgagcgt cacgatggct acaggctccc accgcctctt ccagcagggg 1800
gctattacga agcgcatgac agctatcgag gagatggccg ggatggacgt gctctgctgc 1860
gataagacag gcactctgac cctcaacaag ctgaccgtcg acaagaatct cgtcgaggtt 1920
ttcgcgaagg gggtgggcaa ggagcatgtc ttcctgctcg ctgcccgcgc ctcccgcatc 1980
gagaaccagg acgccattga tgctgctatc gtcggcatgc tggccgaccc aaaggaggcc 2040
agggcgggcg ttcgggaggt gcacttcttc cctttcaacc cggtggacaa gcgcactgcg 2100
ctcacctacg tcgacagcga tggcaattgg catagggctt cgaagggggc cccagagcag 2160
atcctgaacc tctgcaattg caaggaggac gtgcgcagga aggtccacgg cgttatcgat 2220
aagttcgctg agagggggct gaggtcgctc gctgttgccc ggcaggaggt gctggagaag 2280
aagaaggacg ctccaggggg cccgtggcag ctggtcggcc tgctccctct cttcgaccca 2340
cctcgccatg atagcgctga gacgatccgg cgcgctctga acctcggcgt gaatgtcaag 2400
atgattacag gggaccagct ggccatcggc aaggagacgg ggaggaggct cgggatgggc 2460
acaaacatgt acccgtcgtc tgctctgctc ggccaggtca aggactcatc cctgggggcg 2520
ctccctgttg acgagctcat cgagaaggcg gatggcttcg ctggggtttt cccggagcac 2580
aagtacgaga tcgtgcatcg cctgcagcag aggaaccaca tctgcggcat gacgggggac 2640
ggcgtgaatg atgcgcctgc tctcaagaag gccgacatcg gcattgccgt ggtcgacgct 2700
acggatgctg cgaggggggc gagcgatatc gtcctgacag agccgggcct ctcagtcatc 2760
atttccgccg ttctgacctc ccgggcgatt ttccagcgca tgaagaacta cactatctac 2820
gccgttagca tcaccattcg catcgtgttc ggcttcatgt tcattgcgct catctggcag 2880
ttcgacttct cgccgttcat ggtgctgatc attgccatcc tcaatgacgg cacgattatg 2940
acaatctcta aggataggat gaagccgtcc ccacagccag acagctggaa gctgagggat 3000
atcttctcca ctggcgttgt gctggggggc taccaggctc tcatgaccgt cgtgttcttc 3060
tgggtcatga aggacagcga cttcttctcc aactacttcg gcgtcaggcc tctgagccag 3120
aggccagagc agatgatggc tgctctgtac ctccaggtgt cgatcatttc tcaggccctc 3180
atcttcgtca cccggtctcg ctcatggtcc tacgctgagt gcccaggcct gctcctgctc 3240
ggggctttcg tgattgccca gctggtcgcg accttcatcg ctgtgtacgc caactggtca 3300
ttcgcgagga tcgagggggc tggctggggg tgggctggcg tcatttggct gtactccttc 3360
ctcacctaca tcccactcga cctgctcaag ttcggcattc gctacgtgct gagcgggaag 3420
gcctggctca acctcctgga gaataagact gcgttcacca cgaagaagga ctacggcaag 3480
gaggagcgcg aggctcagct ggcggctgcc cagaggaccc tgcacggcct ccagccggcg 3540
gagaagaaca atatcttcaa cgagaagaat agctactcgg agctctcgca gatcgctgag 3600
caggctaagc gcagggccga ggtggtcagg ctgagggaga tcaacacgct caagggccac 3660
gtggagtctg ttgtgaagct gaagggcctc gacattgata ctatccagca gcattacacc 3720
gtctagaacc taaatgctct taactgagct aattatgtaa tgcacataca catatttaca 3780
tagatatgca tatttatata tagcatgtat attgtactac atgcattgct tcttaataca 3840
tgtagtaaag atatatgcaa aaatagtcga aagatttgtt tacatataaa atcaccaata 3900
tttattgtta ttgtattttc atgaataaag taataagatt atttgtctaa tattttgatt 3960
tactagtact agaaatgaaa aggaatatgc acaatttcag cattatagtt tggtaggcaa 4020
aatggagtga gaatagagtt tcatagtata tactaaggtt cttaattgtg caaatagttg 4080
atacaagtca catgggccaa gtttgtaaat cttaaatcga aatatgcctt cttctttttt 4140
tgcatgaaaa tgctagtaat ttataagtgt gtttttcaat aagagatgct aaataccaaa 4200
attaacctag ttttcagtga gcgcttgcat tattgtgg 4238

Claims (12)

1.一种在植物中控制气孔关闭的方法，所述方法包括至少在保卫细胞中通过过表达野生型AHA5或组成型活性的AHA5蛋白以过表达AHA5蛋白，其中所述野生型AHA5蛋白选自SEQID NO:1或SEQ ID NO:2，所述组成型活性AHA5蛋白选自由以下组成的组：来自拟南芥的如SEQ ID NO：23所示的突变AHA5蛋白，来自拟南芥的如SEQ ID NO：30所示的突变AHA5蛋白或来自玉米的如SEQ ID NO：31所示的突变AHA5蛋白。
2.根据权利要求1所述的方法，其包括通过基因编辑技术直接突变植物细胞中的AHA5基因来表达组成型活性的AHA5蛋白。
3.一种鉴定具有控制关闭的气孔的植物的方法，包括在植物群体中鉴定植物的步骤，所述植物过表达如权利要求1中所述的AHA5蛋白或组成型活性的AHA5蛋白。
4.一种培育植物的方法，包括以下步骤：
-播种植物种子，其中所述植物种子源自过表达如权利要求1中所述的AHA5蛋白的植物，和
-从这些播种的种子培育植物，其中培育期在干旱/缺水胁迫条件下进行。
5.根据权利要求4所述的培育植物的方法，其中所述植物种子源自过表达如权利要求1中所述的组成型活性的AHA5蛋白的植物。
6.一种核酸分子，其编码选自下组的组成型活性的AHA5蛋白:
SEQ ID NO：23或SEQ ID NO：30所示的来自拟南芥的组成型活性AHA5蛋白和SEQ IDNO：31所示的来自玉米的组成型活性AHA5蛋白。
7.根据权利要求6所述的核酸分子，其如SEQ ID NO：62，SEQ ID NO：49或SEQ ID NO：50所示。
8.一种核酸构建体，包括在植物中有功能的并允许至少在保卫细胞中表达的启动子，所述启动子与编码如权利要求1中所述的组成型活性的AHA5蛋白的核酸序列可操作地连接。
9.如权利要求8所述的核酸构建体，其如SEQ ID NO：43，SEQ ID NO：44，SEQ ID NO：47，SEQ ID NO：58至61或SEQ ID NO：63至66所示。
10.一种在植物中降低水分损失的方法，所述方法包括过表达如权利要求1中所述的AHA5蛋白的步骤。
11.一种在供水的非胁迫条件或干旱/缺水胁迫条件下提高植物产量或在干旱/缺水胁迫条件下至少维持植物产量的方法，所述方法包括在供水的非胁迫条件或干旱/缺水胁迫条件下培育过表达如权利要求1中所述的野生型AHA5蛋白或组成型活性的AHA5蛋白的转基因植物的步骤，其中：
·与在所述胁迫条件下培育的未过表达所述AHA5蛋白的植物获得的产量相比，在所述干旱/缺水胁迫条件下培育的所述转基因植物获得的产量增加了，或者，
·与在所述非胁迫条件下培育的未过表达所述AHA5蛋白的植物获得的产量相比，在供水的非胁迫条件下培育的所述转基因植物获得的产量增加了，或者，
·与在供水的非胁迫条件下培育的所述转基因植物获得的产量相比，在所述干旱/缺水胁迫条件下培育的所述转基因植物获得的产量至少得以维持。
12.启动子，其序列为SEQ ID NO：40。
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