CN114085852A - 调控植物半矮化株型和叶比值的cll1基因及其豆科的直系同源基因的应用 - Google Patents

调控植物半矮化株型和叶比值的cll1基因及其豆科的直系同源基因的应用 Download PDF

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CN114085852A
CN114085852A CN202110648311.9A CN202110648311A CN114085852A CN 114085852 A CN114085852 A CN 114085852A CN 202110648311 A CN202110648311 A CN 202110648311A CN 114085852 A CN114085852 A CN 114085852A
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陈江华
张晓嘉
方月哏旺
伍青
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Abstract

本发明属于植物的基因技术领域,更具体而言,本发明提供了一种调控植株侧生器官伸长的CLL1基因及其应用。本发明通过对CLL1基因及其编码CLL1蛋白的调控,可以极大增加豆科植物和牧草的叶片/叶柄的比率,同时可以使植株呈现半矮化的表型,从而提高光合效率和最大化利用牧草的可食用部位。

Description

调控植物半矮化株型和叶比值的CLL1基因及其豆科的直系同 源基因的应用
技术领域
本发明属于植物的基因技术领域,更具体而言,本发明涉及调控植株侧生器官伸长的CLL1基因及其直系同源基因以及它们的应用。
背景技术
丝裂原活化蛋白激酶(MAPK)是一种在真核生物中非常保守的丝/苏氨酸蛋白激酶,在许多细胞活动中起作用,如生长增殖,细胞分化,细胞运动或死亡。MAPK级联信号传导由3个不同层次的分子所组成。MAPK被MAPK的激酶(MAPKK)磷酸化后激活,MAPKK被MAPKK的激酶(MAPKKK)磷酸化而激活。而MAPKKK通过与GTPase 和/或其他蛋白酶相互作用而被激活,从而将MAPK和细胞表面的受体以及胞外的信号联系在一起。
在拟南芥中气孔是进化过程中植物由水生到陆生特化出的一个与外界环境气体交换的结构。气孔复合物是由一对保卫细胞和中间的微孔组成,通过气孔的开闭达到与外界进而CO2和氧气的交换。研究证明磷酸激酶通路(MAPK级联通路)可能参与植物气孔早期的发育形成,气孔的发育信号首先由EPF1/EPF2短肽传递,被细胞膜上的受体激酶TOO MANYMOUTHS(TMM)和ER家族的ERECTA(ER)、Erecta-like1 (ERL1)和ERL2传递入细胞,经YODA-MKK4/5-MPK3/6负调控,最终磷酸化核内的SPEECHLESS(SPCH),导致其降解调控细胞气孔的命运。YODA-MKK4/5-MPK3/6通路除调控气孔发育外,在拟南芥中还参与花序、果柄、主根和侧根等植物器官的形态建成。研究表明在该通路上,任意一级成员的缺失,都会导致花序和果柄的缩短,同时畸形花序的形成。此外,Xu和Guo等人分别通过遗传和生化上证明 YODA-MKK4-MAPK6级联通路正向调控水稻种子大小和穗子的发育,从而直接决定水稻的产量。
另一方面,还有研究表明拟南芥中的MKK4/5-MPK3/6通路调控拟南芥花器官的脱落异常。脱落是指被感染或没有功能的器官与母体脱离的现象,脱落对于植物体可以更合理的利用自身资源,从而更好的进行生长发育。遗传证据表明,INFLORESCENCE DEFICIENTIN ABSCISSION(IDA)是该通路的上游,HAESA/HAESA-LIKE2 (HAE/HSL2)单突没有表型,双突表现为花器官不脱落,说明脱落信号的膜上信号的接收需要2个蛋白共同存在。进一步将信号传递到 MAPK级联途径,通过多级磷酸化逐级放大信号。但对于调控花器官脱落的MKK4/5的直接上游和MPK3/6的下游目前还有待进一步的研究。侧根的起始以拟南芥为例可以简化为侧根原基的起始,侧根原基的形成和侧根突破表皮3个过程。在突破表皮的过程中递次要穿过内皮层,皮层和表皮3个部位。Kumpf等人证明IDA-HAE/HSL2突变后侧根数量减少,主要是侧根在突破3层组织部位过程中,细胞壁的果胶降解异常,导致侧根不能正常发育。同时遗传结果表明和生化结果表明, MKK4/5-MPK3/6位于IDA-HAE/HSL2的下游调控侧根的发育,并且细胞壁降解主要是由PG ABSCISSION ZONE ARABIDOPSIS THALIANA(PGAZAT)、PG LATERAL ROOT(PGLR)、XTH23/XYLOGLUCAN ENDOTRANSGLYCOSYLASE 6(XTR6)和EXPANSIN 17(EXP17)的表达影响。
现有的研究结果表明,磷酸激酶通路是植物中非常保守的途径,在植物生长过程中,磷酸激酶通路广泛的参与植物多个方面的生长发育。然而磷酸激酶通路在其他物种中的功能还不是很清楚。植物器官的形态建成,尤其是叶柄和叶轴形态建成背后的分子机制很大程度上是未知的。
发明内容
有鉴于此,本发明提供了一种正向调控植株侧生器官伸长的CLL1 基因及其豆科直系同源基因,通过对CLL1基因的调控,增加叶片/叶柄的比率,同时调整植株的株高,从而提高农作物的光合效率和牧草的利用率。
在第一方面,本发明提供了一种突变的蒺藜苜蓿CLL1基因,与 Medtr1g100250相比,所述突变的蒺藜苜蓿CLL1基因第一内含子缺失,所述缺失的第一内含子序列如SEQ IDNO.1所示。所述突变的蒺藜苜蓿CLL1基因的核苷酸序列如SEQ ID NO.2所示,其编码的CLL1蛋白序列如SEQ ID NO.3所示。
在第二方面,本发明提供了本发明第一方面所述CLL1蛋白在调控植物侧生器官伸长中的应用,所述调控为正向调控。
在第三方面,本发明提供了一种蒺藜苜蓿cll1突变体,其特征在于,与野生型相比,所述cll1突变体中包含本发明第一方面所述突变的蒺藜苜蓿CLL1基因,其核苷酸序列如SEQ ID NO.2所示。
在第四方面,本发明提供了一种改良植物的方法,所述方法包括调低CLL1基因表达,例如去除所述CLL1基因的第一内含子或者用 RNA干扰技术降低CLL1基因表达。
在一些实施方案中,用于RNA干扰的片段如SEQ ID NO.4所示。
在第五方面,本发明提供了一种融合表达载体,所述融合表达载体包括待表达基因序列和插入替换的所述CLL1基因的第一内含子。
在一些实施方案中,所述融合表达载体中的所述待表达基因序列如SEQ ID NO.5所示;所述CLL1基因的第一内含子序列如SEQ ID NO.1所示。
在第六方面,本发明提供了第二方面所述的CLL1蛋白的豆科直系同源蛋白,所述蛋白的氨基酸序列如SEQ ID NO.6-20所示,所述豆科植物包括:鹰嘴豆(Cicerarietinum)、豌豆(Pisum sativum)、大豆 (Glycine max)、菜豆(Phaseolus vulgaris)、赤豆(Vigna angularis)、落花生(Arachis hypogaea)、蔓花生(Arachis duranensis)、绿豆(Vigna radiata)车轴草(Trifolium pratense)、紫花苜蓿(Medicago sativa)和百脉根(lotus japonicus)。
在第七方面,本发明提供了所述CLL1蛋白的豆科直系同源蛋白的编码基因,所述基因的核苷酸序列如SEQ ID NO.21-35所示。
本发明的有益效果:
1)提供了一种正向调控植株侧生器官伸长的突变的CLL1基因,可用于调控植株的生长发育,特别是增加叶片/叶柄的比率和使植株呈现半矮化的表型;
2)提供了CLL1基因在其他重要的豆科作物中的直系同源基因及其编码的蛋白在调控植物侧生器官伸长中的应用;
3)提供了一种通过调控CLL1基因及其豆科直系同源基因来改良植物的方法,所述方法可以极大增加豆科植物和牧草的叶片/叶柄的比率,同时植株呈现半矮化的表型,从而提高作物产量和最大化牧草的可食用部位(叶片),为分子育种提供参考资源。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本发明的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他的实施方案。
图1示出了CLL1的分子克隆;其中,图1A示出了CLL1位点首先被定位到1号染色体的长臂上,位于标记fgp2468和456-2之间,并进一步限定到fgp2468和fgp2472之间约120kb的区域;图1B示出了位于120kb的区域内的17个候选基因,第13个基因为 Medtr1g100250;图1C示出了野生型和cll1突变体植株中CLL1的基因结构,黑框和线条分别表示外显子和内含子,红线代表CLL1中的第一内含子,基因结构上下的箭头代表不同的引物;图1D示出了野生型和 cll1突变体植株中CLL1 gDNA部分序列的PCR扩增;图1E示出了野生型和cll1突变体中CLL1转录物的RT-PCR分析;图1F示出了野生型和cll1突变体中CLL1的相对表达水平,MtGAPDH基因用作内部对照;数据取平均值(+SD),n=3(图1F);*代表学生t检验的统计显著差异(*,P<0.05;**,P<0.01),以上实验均重复三次,结果相似。
图2示出了CLL1的第一内含子刺激LUC活性;图2A示出了 CLL1启动子LUC结构和将CLL1的第一内含子异源连接在拟南芥TRP1 后面的示意图,中空框分别代表CLL1和TRP1的启动子区域,红色短框代表CLL1的第一内含子,灰框代表AtTRP的部分编码区域(CLL1 的第一内含子插在AtTRP的编码区域内),黑框代表LUC基因,pCLL1:: LUC缩写为CLL1-p,pCLL1-Intron::LUC缩写为CLL1-p-intron1;图 2B和图2D示出了CLL1-p-intron1和AtTRP-p-intron1在烟草叶中的反式激活;图2C和图2E示出了萤火虫荧光素酶(LUC)与海肾荧光素酶(REN)活性的比率;数据取平均值(+SD),n=3(图2C和图2E); *代表学生t检验的统计显著差异(*,P<0.05;**,P<0.01),以上实验均重复三次,结果相似。
图3示出了CLL1基因表达降低的转基因株系中的叶柄和叶轴;其中,图3A示出了代表的野生型、RNAi-1和RNAi-2转基因株系(7 周)的成熟复叶;图3B示出了野生型、RNAi-1和RNAi-2的7周龄植株第4节的叶柄表面SEM图;图3C示出了野生型、RNAi-1和RNAi-2 植株7周龄植株第4节的叶轴表面SEM图;图3D和图3E分别示出了野生型、RNAi-1和RNAi-2植株中CLL1和Medtr8g098425的相对表达水平,其中野生型中的CLL1和Medtr8g098425的表达水平值被设置为 1,数据取平均值(+SD),n=3(图3D和图3E),MtGAPDH基因用作内部对照;图3F示出了7周龄植株成熟复叶的叶柄长度;图3G和图 3H分别示出了7周龄植株第4节上成熟复叶的叶柄表皮细胞长度和叶柄细胞数;图3I示出了7周龄植株成熟复叶的叶轴长度;图3J和图3K 分别示出了7周龄植株第4节上成熟复叶的叶轴表皮细胞长度和叶轴细胞数;数据取平均值(+SD),n≥9(图3F、图3H、图3I和图3K),n≥28 (图3G和图3J);*代表经学生t检验,野生型与RNAi-1或RNAi-2 之间的差异有统计学意义(*,P<0.05;**,P<0.01),ns代表不显著,刻度尺为2cm(图3A)和20μm(图3B-图3C)。
图4示出了CLL1基因表达降低的转基因株系的植株高度(10 周);其中,图4A示出了野生型、RNAi-1和RNAi-2转基因株系的植株高度的照片;图4B示出了野生型、RNAi-1和RNAi-2转基因株系的植株高度的对照图;数据取平均值(+SD),n=8;*代表经学生t检验,野生型与RNAi-1或RNAi-2之间的差异有统计学意义(*,P<0.05;**, P<0.01)。
图5示出了野生型和过表达的转基因株系(35S::N-CLL1#1和 35S::N-CLL1#2)的植株高度的照片。
图6示出了CLL1控制叶柄和轴中的细胞增殖和细胞伸长相关基因;其中,图6A示出了通过对cll1突变体的叶柄进行RNA-seq分析揭示出的相关基因表达(MtCYCs和MtEXPs);图6B和图6C分别示出了野生型和cll1突变体的叶柄和叶轴中MtCYCs的相对表达水平;图6D- 图6I分别示出了野生型和cll1突变体的叶柄和叶轴中MtEXPs的相对表达水平,在图6B-图6I中,数据取平均值(+SD),n=3,MtGAPDH 基因用作内部对照,野生型中的MtCYCs和MtEXPs的表达水平值被设置为1;*代表学生t检验的统计显著差异(*,P<0.05;**,P<0.01),以上实验均重复三次,结果相似。
具体实施方式
下面将结合本发明的实施方案和附图,对本发明进行清楚、完整的描述。显然,所描述的实施方案仅仅是本发明的一部分实施方案,而不是全部的实施方案。基于本发明中的实施方案,本领域普通技术人员可以获得的所有其他实施方案,都属于本发明保护的范围。
实验方法
1.植株生长条件
所有实验均在温室中进行,条件如下:16小时/8小时昼夜(温度为22℃/18℃),光照强度为150μEm-2秒-1,35%-40%的相对湿度,给植物进行充分浇水,并且每隔2周施用0.1%的水溶性肥料(Peters Professional Allrounder,20-20-20+TE)。
2.扫描电子显微镜(SEM)
收集7周龄植物的成熟组织(叶柄、叶轴和节间),在FAA固定溶液(5%甲醛、5%乙酸和50%乙醇)中真空渗入3次,约30分钟,然后于室温保持72小时。随后依次在50%的乙醇(30分钟)、60%的乙醇(30分钟)、70%的乙醇(30分钟)、80%的乙醇(30分钟)、85%的乙醇(15分钟)、90%的乙醇(15分钟)、95%的乙醇(15分钟)、100%的乙醇(15分钟)和100%的乙醇(1小时)中脱水。最后进行SEM(He et al.,2020)。所有样品都在Zeiss EVO LS10(CarlZeiss,Oberkochen) 并且加速电压为5千伏的条件下进行检测。细胞长度和细胞数用ImageJ软件计算。
3.RNA提取和实时荧光定量PCR(qRT-PCR)
使用RNeasy Mini试剂盒(Tiangen)从各种组织中提取总RNA。使用1.5μg的总RNA通过
Figure RE-GDA0003265751600000072
II第1链cDNA合成试剂盒 (Takara,Shiga,Japan)进行cDNA合成。使用25ng-50ng的RNA 作为模板,在Roche LightCycler480II仪器上进行qRT-PCR(Zhang etal., 2020)。PCR试验被设置为:阶段1,于95℃(3分钟);阶段2(28个循环),95℃(30秒),58℃(30秒),72℃(2.5分钟);阶段3,72℃ (5分钟),通过RT-PCR扩增CLL1转录物。MtGADPH用作内部对照,并进行三次生物学重复。使用的引物序列如表1所示。
表1.引物序列
Figure RE-GDA0003265751600000071
Figure RE-GDA0003265751600000081
*FW为正向引物,Rv为方向引物
4.质粒构建
将来自野生型的cDNA的CLL1编码序列进行PCR扩增,然后克隆到pCAMBIA3301NM载体的Pm1I和BstEII位点,生成质粒p35S::GFP-CDS。为了生成质粒p35S::GFP-CDSK440M,分别从质粒 p35S::GFP-CDS中PCR扩增CLL1的N-片段和C-片段(Xu et al.,2018)。 CLL1的N-片段和C-片段经重叠延伸PCR重组在一起,然后重组片段被克隆到pCAMBIA3301NM载体的Pm1I和BstEII位点生成质粒 p35S::GFP-CDSK440M。将来自野生型的4071bp的天然启动子进行PCR扩增,然后克隆到pCAMBIA3301载体的BamH1和NcoI位点,生成质粒pCLL1::GUS。将来自质粒pCLL1::GUS的相同序列进行PCR扩增,并克隆到pGreenII 0800-Luc载体的HindIII和BamHI位点,生成质粒 pCLL1::LUC。为了生成质粒pCLL1-intron::LUC,将来自质粒 pCLL1::GUS的自身启动子进行PCR扩增,并且将来自野生型gDNA 的CLL1的第一内含子进行PCR扩增。自身启动子和CLL1的第一内含子在融合反应中经PCR扩增,然后重组片段被分别克隆到pCAMBIA3301NM和pGreenII 0800-Luc载体的。为了生成质粒 pYLRNAi.2-CLL1,将来自野生型的cDNA的CLL1的C末端的一个非保守区(321bp)进行PCR扩增并且克隆到pYLRNAi.2载体中。所述方法参见现有技术(Hu and Liu,2006)。
除了pYLRNAi.2-CLL1,本研究中使用的所有构建体均使用一步克隆试剂盒(Vazyme)制备。将重组质粒导入根癌农杆菌(Agrobacterium tumefaciens)EHA105。所使用的引物序列如表1示。
5.反式激活活性分析
CLL1-p、CLL1-p-intron 1、AtTRP1-p和AtTRP1-p-intron 1构建体通过根癌农杆菌EHA105渗透导入3周龄的烟草叶。使用双荧光素酶检测试剂盒(Promega)在渗透后2天记录萤火虫荧光素酶(LUC)与海肾荧光素酶(REN)活性(He et al.,2020)。
6.RNA测序
从野生型和cll1突变体植株收集第7个时期(原基期)的叶柄。使用Trizol试剂盒(Invitrogen,Carlsbad,CA,USA)提取总RNA,并通过Agilent 2100生物分析仪(AgilentTechnologies,Palo Alto,Ca, USA)和琼脂糖凝胶电泳来证实RNA质量。用短cDNA片段(200bp-700 bp)组装准备RNA测序文库,并在Illumina Hiseq2500(广州基迪奥生物科技有限公司)上进行测序。去除低质量的碱基和接头后,高质量的干净读数被映射到蒺藜苜蓿基因组版本Mt4.0 (http://www.medicagogenome.org/)使用HISAT2.2.4软件(Kim et al.,2015)。通过DESeq2软件(Love et al.,2014)和Edger软件(Robinson et al.,2010)对差异表达基因进行分析和分离,结果显示倍数变化≥2和假发现率<0.05。
实验结果和分析
1.cll1突变体的分离
发明人使用正向遗传筛选从美国诺贝研究所(Noble Research Institute)蒺藜苜蓿(Medicago truncatula)(A17)的快中子诱变突变体库中筛选到1个叶柄和叶轴都缩短的突变体株系(FN8157),该突变体同时呈现半矮化的表型。经遗传试验验证该突变体为隐形纯合单核基因控制的突变体,因此发明人将该突变体命名为clustered leaf 1(cll1)。除了与RNAi转基因株系(R108)相比,本文所述的野生型(WT)均为A17生态型。
2.cll1突变体的特征描述
发明人通过实验研究发现,与野生型相比,cll1突变体的叶柄和叶轴长度不同,并且叶柄和叶轴的细胞伸长和增殖均异常。
总的来说,cll1突变体有以下几个特征:
①叶柄和叶轴细胞长度缩短:相对于野生型蒺藜苜蓿,cll1突变体通过细胞学的观察叶柄和叶轴细胞长度都显著的缩短。
②叶柄和叶轴细胞数目减少:通过计算结果表明,cll1突变体叶柄和叶轴表皮细胞的数目也显著低于野生型。
③株高缩短:发明人还观察到了cll1节间长度缩短,经细胞学观察缩短可能是因为细胞增殖都异常导致。
综上所述可以看出,cll1突变体侧生器官长度改变的主要是由侧生器官细胞伸长异常或增殖异常所导致的。
3.CLL1基因克隆及基因表达
①材料收集与回交:将cll1突变体与野生型(A17)进行杂交, F1为野生型表型,通过收集大量自花授粉的F1植株的种子(F2),进一步进行分离。F2植株中的突变体与野生型的比率为34:100(1:3),表明CLL1是一个单隐性核基因。②图位克隆:通过图位克隆技术,先后使用699个和1632个F2分离的纯合突变体(图1A)逐步将CLL1 基因定位在1号染色体上的简单重复序列(SSR)标记fgp2468和fgp2472之间的120Kb的区间,该区间共有17个候选基因。
③候选基因确定:发明人在120Kb的候选区间确定了17个候选基因。进一步对第7个时间发育时期的野生型和突变体叶柄进行了转录组测序,结果发现该区间只有3个基因表达量显著变化,并且只有第13个基因(Medtr1g100250)表达量显著下调,因此我们将cll1突变体中Medtr1g100250作为唯一的候选基因。首先发明人通过测序发现该基因第一内含子完全删除(图1C-图1D),并且除第一内含子外其他基因组序列并没有差异。与RNA-seq数据一致的是,RT-PCR分析几乎无法检测到Medtr1g100250完整编码序列 (CDS)的转录物(图1E),表明Medtr1g100250的表达被破坏(图 1E)。qRT-PCR分析表明野生型中Medtr1g100250的转录表达水平比突变体中的高约12倍,突变体中的Medtr1g100250的表达量相对于野生型显著性下降(图1F)。通过比对野生型和cll1突变体的编码序列,发现Medtr1g100250的编码序列并没有改变。
为了确定Medtr1g100250的第一内含子促进基因表达。发明人还使用了重组LUC报告结构进行了测试,其中将LUC融合在4kb 的CLL1启动子和471bp的拟南芥TRP1启动子下游,并且包括第一内含子(图2A)。在两组实验结果中,第一内含子都显著刺激LUC 报告基因的活性(图2C和图2E)。
因此,第一内含子是Medtr1g100250的正常表达所必需的,而 cll1是可能由于Medtr1g100250的第一内含子的缺失导致表达量下降的突变体。
④遗传验证:为了证明Medtr1g100250的表达异常是导致cll1 突变体表型的原因,发明人试图通过反向筛选分析从蒺藜苜蓿的 Tnt1突变体库搜索到Medtr1g100250有插入的突变体,但并没有找到。然后,为了进一步证实cll1表型的缺陷与Medtr1g100250基因表达相关,发明人通过RNAi(RNA干扰技术)来抑制Medtr1g100250 的表达。p35S::CLL1-RNAi-1(缩写为RNAi-1)和p35S::CLL1-RNAi-2 (缩写为RNAi-2)转基因株系具有与cll1植株相似的短叶柄和短叶轴(图4A),并且Medtr1g100250的表达量显著降低(图3D)。SEM 和转基因统计结果表明,RNAi-1和RNAi-2植株的叶柄和叶轴的细胞伸长和细胞增殖均异常,与野生型和cll1植株相似(图3B-图3C 和图3F-图3K)。此外,除叶柄和叶轴外,转基因株系的株高也与对照组有显著差异(图4)。另一方面,发明人通过删除CLL1基因 N端的抑制域,将删除N端抑制域后的编码序列命名为N-CLL1。然后将该段序列连接到35S启动子后来驱动该段序列,从而使 Medtr1g100250基因过表达。过表达的转基因植株(35S::N-CLL1 #1和35S::N-CLL1#2)的叶柄和叶轴相对于野生型显著伸长(图 5)。
这些结果证实了Medtr1g100250即为CLL1基因,并且cll1突变体是由CLL1基因的表达量下调导致。CLL1基因突变导致侧生器官缩短,而CLL1基因过表达导致侧生器官伸长。
4.CLL1表达模式及其蛋白亚细胞定位
进化分析和氨基酸序列比对分析表明CLL1蛋白具有高度保守的激酶结构域(kinase domain),说明CLL1蛋白可通过激酶结构域与其他相关蛋白相互作用,来调控植株的生长发育。
此外,qRT-PCR结果显示,CLL1在幼叶、果夹、茎和授粉后的种子等组织中表达量较高,同时在叶柄和叶轴中也有一定的表达(图 6D)。
在花椰菜花叶病毒35S启动子(CaMV35S)的控制下,通过在烟草的叶中瞬时表达CLL1与绿色荧光蛋白的融合蛋白 (GFP-CLL1K440M),以确定CLL1蛋白亚细胞定位。检测结构显示 GFP-CLL1K440M融合蛋白均位于烟草表皮细胞质和细胞核。这些数据表明CLL1蛋白是一种保守的MAPKKK蛋白。
5.CLL1参与信号通路以调控叶柄和叶轴的发育
现有文献表明YODA作为磷酸激酶途径中关键的一个环节,接受上游信号,继而磷酸化下游的MKK家族的蛋白激酶,继而将信号传递下去。并且在拟南芥和水稻中YODA-MKK4/5-MAPK3/6和 OsMKKK10-OsMKK4-OsMAPK6这两条通路已经共同证明MKK4/5 为下游传递信号的关键蛋白。然而,现有文献表明在蒺藜苜蓿中只进化出一个MtMKK4,来替代拟南芥中的AtMKK4和AtMKK5。并且在调控蒺藜苜蓿的根瘤和育性上已经证明MtMKK4和下游的MtMAPK3/6存在蛋白上的互作。因此,发明人推测CLL1-MtMKK4 级联可能是蒺藜苜蓿中的保守信号通路。
为了完善蒺藜苜蓿中这一信号通路,发明人通过酵母双杂 (Y2H)、双分子荧光互补和融合蛋白沉降技术证实了CLL1蛋白与 MtMKK4之间的相互作用。此外,发明人还在体外进行了蛋白磷酸化试验证明CLL1蛋白可以磷酸化MtMKK4。因此CLL1-MtMKK4 信号通路可能参与正向调控蒺藜苜蓿的叶柄和叶轴的发育。
6.CLL1影响细胞周期并调控基因的表达
植物器官的发育受到细胞伸长和细胞分裂两个方面的协同调控。为了解析CLL1调控叶柄和叶轴伸长的机制,发明人收集发育过程中第7个时期的野生型和cll1植株的叶柄进行RNA-seq。与野生型的叶柄相比,cll1突变体中1218个基因显著上调,1187个基因下调。此外,KEGG通路的分析结果表明,与油菜素甾醇生物合成(ko00905) 和MAPK信号通路(ko04016)相关的基因得到了富集,这些基因涉及植物发育。
RNA-seq结果及qRT-PCR共同证明在cll1植株的叶柄中 MtCYC2;2和MtCYC2;3等细胞周期相关的基因表达量全部显著下调(图6B和图6C),另外MtEXP13、MtEXP11、MtEXPB3和MtXTH8 等细胞伸长相关的基因表达量也全部下调(图6D、图6E、图6G和图6H)。因为叶轴发育过程类似于叶柄,因此发现叶轴中MtCYC2;3 的基因表达量显著下调(图6B),同时MtEXP13、MtEXP11、MtEXPR、 MtEXPB3、MtXTH8和MtCESA3的基因表达量全部显著下调(图6D-图6I)。
这些结果表明,CLL1在蒺藜苜蓿的叶柄和叶轴中都影响核心细胞周期和细胞伸长的基因表达,进而调控器官大小。
7.CLL1及其相应豆科直系同源基因
通过进化分析表明,CLL1在其他重要的豆科作物,如、鹰嘴豆、豌豆、大豆、菜豆、赤豆、落花生、蔓花生、绿豆、车轴草、紫花苜蓿和百脉根中具有保守的同源基因(参见表2)。
表2.蒺藜苜蓿中CLL1基因及相关物种中CLL1豆科同源基因
Figure RE-GDA0003265751600000141
Figure RE-GDA0003265751600000151
CLL1及其相应豆科直系同源基因在不同物种中参与侧生器官伸长调控的证据如下:
1)根据CLL1蛋白及相应物种中同源蛋白的进化分析,从进化树可以看出所有蛋白具有同源性,聚集在相应的分枝上,这从一方面说明其功能的保守性;
2)对CLL1蛋白及相应物种中同源蛋白进行蛋白保守结构域分析,分析结果显示:CLL1及其相应的豆科同源蛋白都具有保守的激酶结构域(kinase domain);功能保守的特定家族的蛋白一般依赖于其特定的保守结构域来发挥其功能,根据现有文献中YODA–MKK4/5–MAPK3/MAPK6和 OsMKKK10-OsMKK4-OsMAPK6这两条通路在调控花柄伸长、气孔形成、株型形成、主根、侧根和种子伸长上有保守的机制。所以从 CLL1及其同源蛋白的蛋白结构保守性,可能也参与苜蓿中类似的信号通路来调控器官发育这功能上的保守性;
由此可见,在多个物种中CLL1或是其同源基因对侧生器官具有正调控功能,这是CLL1及其同源基因功能保守性最直接的证据。
通过调控CLL1基因及其同源基因可以直接调控植物侧生器官的伸长,极大地增加植物的叶片/叶柄的比率,同时可以调整植物的株高。对于豆科植物,增加叶片/叶柄的比率可以增加单位面积的光合效率从而增加产量。同时对于牧草,又可以极大增加的可食用部位产量(牧草中叶柄的蛋白含量远低于叶片,并且叶片为主要的食用部位)。半矮化的株型对于豆科作物可以合理的密植,从而增加其产量。所以CLL1与其同源基因在分子育种方面具有较大的运用潜力及价值。
表3.相关序列
Figure RE-GDA0003265751600000152
Figure RE-GDA0003265751600000161
Figure RE-GDA0003265751600000171
Figure RE-GDA0003265751600000181
Figure RE-GDA0003265751600000191
Figure RE-GDA0003265751600000201
Figure RE-GDA0003265751600000211
Figure RE-GDA0003265751600000221
Figure RE-GDA0003265751600000231
Figure RE-GDA0003265751600000241
Figure RE-GDA0003265751600000251
Figure RE-GDA0003265751600000261
Figure RE-GDA0003265751600000271
Figure RE-GDA0003265751600000281
Figure RE-GDA0003265751600000291
Figure RE-GDA0003265751600000301
Figure RE-GDA0003265751600000311
Figure RE-GDA0003265751600000321
Figure RE-GDA0003265751600000331
Figure RE-GDA0003265751600000341
序列表
<110> 中国科学院西双版纳热带植物园
<120> 调控植物半矮化株型和叶比值的CLL1基因及其豆科的直系同源基因的应用
<160> 68
<170> SIPOSequenceListing 1.0
<210> 1
<211> 134
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
gttgcctcct caaaccataa cacgcttcca cattgtacat gttattgcat atgcatgcct 60
taattatttg tgattgattg cattagaaat acgtatcttt tcaacttcac tcaaaagttt 120
ccctgtttct gaag 134
<210> 2
<211> 4725
<212> DNA
<213> 蒺藜苜蓿(Medicago truncatula)
<400> 2
atgcctacat ggtggggaaa atcatcatcg aaagaaacca agaagaaagc aggtaaggaa 60
agtattattg acacattgca cagaaaattt aaatttccat ctgagggtaa acgaagcact 120
atatctggag gatctcgtag acgaagcaat gacacaattt cagagaaggg ggaccgttct 180
ccatctgaat caagatcccc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggcctcatg ctcaacccct tccacttccg ggtttgcacc cttcaagttt aggtcgagtt 300
gattctgaaa ttagtatatc agcgaaatcg agactggaaa agccctccaa gccgtcatta 360
tttcttccac ttccaaaacc ttcatgcata cgttgtgggc caactcctgc agatttagat 420
ggagatatgg tcaatgcttc agtcttcagt gattgctctg ctgatagtga cgagccagca 480
gactcacgca accgtagtcc tctggcaact gactcggaaa ctggtacaag aactgctgcc 540
ggcagtcctt ccagcttggt gctcaaggat caatcatctg ctgtttccca accgaatttg 600
agagaagtaa aaaaaacggc aaccattctc agtaatcaca caccttctac ttcaccaaaa 660
cggaagccat tacgccacca tgttccaaat cttcaagttc cacctcatgg tgtcttctat 720
agtggtcccg acagttcctt gtcaagtcca tcaagaagtc cgttgagggc atttggtaca 780
gatcaggtgt tgaactctgc tttttgggct ggaaagccat atccagagat caatttcgtc 840
ggatctggcc actgctctag tccaggttct ggtcacaatt ctgggcataa ttcaatggga 900
ggggacatgt caggaccatt attttggcaa ccaagcaggg gtagccctga gtattctcca 960
atacctagcc ctagaatgac tagccctggt ccaagctcaa gaattcagag tggagcagtc 1020
acacctattc atcctagagc agggggaaca ccaactgaat cacagacagg acgggctgat 1080
gatggaaaac aacaaagtca tcgtttgccc ctccctcctt taacagttac caatacctcg 1140
ccattctctc attctaattc tgcagcaaca tctccatcta tgccgagaag tccagctaga 1200
gcagatagtc caatgagctc tgggtcacgt tggaaaaaag gaaagctgct tggcagaggt 1260
acatttgggc acgtctatat tggcttcaat aggtatgtac cctggataaa atgtaaacat 1320
ttcatcattt tttttaggat taatttctat ttatgcatct taaaaatact gatgtggtta 1380
aaaaatgttt gtctctctta gtcaaagtgg tgaaatgtgt gcaatgaagg aggttactct 1440
gttttcagat gatgccaagt ctttggaaag tgctaagcaa ttgatgcagg tatactgttc 1500
ccttctaaat ctagcaaaaa cttcacactg ataaacagtt cattttcgtt aggtgcaata 1560
ctatattttc tagtcttgaa ttcaacacag ctttcaaaat ctaactacaa gatttctgac 1620
aggaagttca tttattaagc cgtttaagac atccaaatat tgtgcagtat tatggctctg 1680
aaacagtaag ttctctcctt cgatatgatg gcgaatgacg acgatttatt tttgattcta 1740
ttatagactg tgcattttta atggatgcat gatcttctag tcacaagcat attagttagt 1800
ttgtggaagt ttacctaaca agaacaatat agtggcagaa atttgttagc attatttgtt 1860
tctaaagtct acttatagaa gtttttggat catcccctgc acaggtagat gacaagcttt 1920
acatatacct tgagtatgta tctggaggtt ccatacataa acttcttcaa gaatatgggc 1980
aatttggtga actagctatt cgaagttata ctcaacaaat tttgtcaggg cttgcttatt 2040
tacatgctaa aaatactctt cacaggtgaa ctaaggcatc ataatctcaa tgttttaaat 2100
tttgctgctg ccacatattt agtttctttt ttaaacttct gacatatcgt tctctgaaat 2160
tccatgtaca tgtttaggga catcaaagga gcaaacatat tggtagatcc aaatggtcgg 2220
gtcaaggttg cagactttgg catggcaaaa catgtaagct tagagatgat aactgtccgg 2280
tttccattat attttcattt agctgactta actagatatg aaaattagat tttttttttg 2340
accaaaaaaa ttagattcta atgctgattg aaattataaa tcataggttt ttttctaaaa 2400
aatctcaata tatcaatgtt tctggcttct tgatagcaat tgggtgattt aaaatgttaa 2460
aagaaaatgt gaagatggaa ccctattttt aagtatggaa agagaaacca gaataataca 2520
aaattgtgat ggtgtatgat ttaccttgta aaccacaatt tatgctgtcc aggatgtgca 2580
tgatttgtcc catttgttgt acttacaatt acatgattca cagataacag ggcaatactg 2640
tccattgtcg ttcaaaggaa gtccttattg gatggctcct gaggttctac tttttctttt 2700
ttgggttcag ttttttagat tttaattgca ttttagtttt gtcactcatt atcttttctt 2760
tttgatgatt caggttataa agaactccaa ggaatgcagc cttggtgtgg atatatggag 2820
tcttggatgc acagttttgg aaatggctac aacgaagcct ccttggtctc agtatgaagg 2880
ggtaagtaat tcatgtatat acatgagatt ttatatttac gaagacctgg tgatgttttt 2940
gtattataaa aatgtcaact ttttaaatta attcttctta ctgctttgtg tatttacgtg 3000
ttactataac aggttgctgc catgttcaaa attggtaaca gcaaggagct cccaacaatt 3060
ccagatcatc tctcaaatga aggaaaagat tttgttagaa aatgtctaca acgtaatcca 3120
cgtgatcgcc cttcagcaag tgaattatta gaccaccctt ttgtaaaagg tgctgcacct 3180
ttggaaagac ctattatggt tcccgaagct tcagacccta ttactggaat tacacatgga 3240
acaaaagctc tggtgcgtaa cctatttcct actccaatcg ttggaaactc acttcagttg 3300
tgtttgtgtg gttgttcatg ttaccattcg atggtacttt gtctttcatt ttatgcgaac 3360
atcaaactgt tgcatttgcc ttatctacac tattgaacaa aaaaggcgac agtttgctct 3420
cttcatgaaa atgtcatgtg aacttctgtt cttgttttca catttctcat gattgagtaa 3480
tttaagcggc gtgaatattt tataggctat tcacgctctt agtaatacat aaatgtaaag 3540
attttgttct gtcaagtcca aaatatcgag tcaattgcgt gatctatatt ttcattcatt 3600
tttctgtgaa agttattaag gaaattgttc tttgcatgag gcagcatgaa tgatgcataa 3660
gggaaaaaca tagacgatag ctcaagacat agtttaggtt gagggatagt gagatattgg 3720
ttggaaagcg ataaggttct agggttttta aattggtttc ccttttcatc agatctggct 3780
agaattttat gtcaagagta agtatacaca tggagttcag ttaggatgga gacttttgtt 3840
atttcaattc agtataattt attttaaaag taatataaat cttgtttcgt gtttattagc 3900
atgaagtatc ttccaaatat gaggtcacaa caagtttttc ttgcaagctt tgtactactc 3960
aatctgtatt ctcttgtttt atttttataa taattgcatc aatttcaggg cattggacaa 4020
ggaaggaatc tgtctgcctt ggattcagat aaactcttgg ttcattcttc tagggttttg 4080
aaaaataatc ctcatgaaag gtatctctct agctcactca cccacacaca tacacacaca 4140
aaaatgcatg caaggctgtt gacacattgc caatggtaat ctgaaatcat ttcatcaatt 4200
tccagtgaaa tccatattca aaggaatata tcttgcccag tctctcccat tggaagccca 4260
cttttgaggt caagatcacc acagcagagg agtgggagat tgtctccttc tcctatatct 4320
agccctcgga ccgcttctgg tgcatccacg cctctcactg gcggcagtgg tgccattcca 4380
ttcagtaacc acttaaaaca atcagtttac tttcaagagt gtctcggaag catgccgaaa 4440
tccccaaatg gtgtctacat taacggctct tctcaccacg attcaaatat cgacattttt 4500
caaagaatgc aagccgggtc tcacattaaa tcagagctag tctccagtga taatgatgct 4560
cttggtaagc agtttgtaag gtctcctcat gcagaaccgt atgactttca gtcggtctta 4620
gctgatcgtg ttggccggca gcttctgggg gatcatgtta agattaaccc atcatttgat 4680
ccaagtccca gctcgtcgat gctcaaccgg acaaatggtt tatga 4725
<210> 3
<211> 899
<212> PRT
<213> 蒺藜苜蓿(Medicago truncatula)
<400> 3
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Arg Ser Thr Ile Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Leu Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ala Lys Ser Arg Leu
100 105 110
Glu Lys Pro Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ser
115 120 125
Cys Ile Arg Cys Gly Pro Thr Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Val Leu Lys Asp Gln Ser
180 185 190
Ser Ala Val Ser Gln Pro Asn Leu Arg Glu Val Lys Lys Thr Ala Thr
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Lys Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Ile Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Arg Ala Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Gln
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Val His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Thr Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Leu Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Asn Asn Pro His Glu Ser Glu Ile His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
Gln Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Gly Ala Ile
770 775 780
Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu
785 790 795 800
Gly Ser Met Pro Lys Ser Pro Asn Gly Val Tyr Ile Asn Gly Ser Ser
805 810 815
His His Asp Ser Asn Ile Asp Ile Phe Gln Arg Met Gln Ala Gly Ser
820 825 830
His Ile Lys Ser Glu Leu Val Ser Ser Asp Asn Asp Ala Leu Gly Lys
835 840 845
Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val
850 855 860
Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile
865 870 875 880
Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Ser Met Leu Asn Arg Thr
885 890 895
Asn Gly Leu
<210> 4
<211> 321
<212> DNA/RNA
<213> 人工序列(Artificial Sequence)
<400> 4
ggaaggaatc tgtctgcctt ggattcagat aaactcttgg ttcattcttc tagggttttg 60
aaaaataatc ctcatgaaag tgaaatccat attcaaagga atatatcttg cccagtctct 120
cccattggaa gcccactttt gaggtcaaga tcaccacagc agaggagtgg gagattgtct 180
ccttctccta tatctagccc tcggaccgct tctggtgcat ccacgcctct cactggcggc 240
agtggtgcca ttccattcag taaccactta aaacaatcag tttactttca agagtgtctc 300
ggaagcatgc cgaaatcccc a 321
<210> 5
<211> 330
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
atggttattg cggtggcgac gacgagctcc attgtttccg gaatcaagct ttcagggatc 60
ttaacttcgt tcaacgccgt agatgatgcg tccagtagtt gcggaagaag caacttgacc 120
ggagttagaa tttttccgac attgagtcgg cgacgattct catcgatcgg agctgtttcc 180
ccaattcgtg gggatgctca atcttccttt agtcgcagct ctttcgcttg ctctcagaat 240
ctcggtttga gcggtggatt tagtgcagct gaagctctgc cacctgcttg tgcaaatgct 300
agcccttctt ccattaaatc tttcaaccag 330
<210> 7
<211> 882
<212> PRT
<213> 鹰嘴豆(Cicer arietinum)
<400> 7
Met Pro Ser Trp Trp Gly Lys Leu Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Ser Lys Glu Ser Ile Phe Asp Thr Leu His Arg Lys Phe Arg Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Ile Ser Gly Glu Ser Arg Arg Arg
35 40 45
Cys Ser Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ser Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Val Lys Ser Arg Leu
100 105 110
Glu Lys Ser Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ala
115 120 125
Cys Ile Arg Cys Gly Pro Ser Pro Ala Asp Leu Asp Gly Asp Leu Val
130 135 140
Thr Asn Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Met Leu Lys Asp Gln Thr
180 185 190
Ser Ala Val Ser Gln Leu Asn Ser Arg Glu Val Lys Lys Pro Thr Asn
195 200 205
Ile Leu Ser Asn His Thr Ser Ser Thr Ser Pro Lys Arg Arg Pro Leu
210 215 220
Arg Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Val Asn Phe Leu Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Ala Gly Thr Pro Thr
340 345 350
Glu Ser Gln Ser Gly Trp Val Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Ser Ser Leu Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Glu
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Met Phe Ser Asp Asp
435 440 445
Ala Lys Ser Met Glu Ser Ala Lys Gln Leu Met Gln Glu Ile His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Gly Cys Ser Leu Ala Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Ala Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ile Glu Leu Leu Asp His Pro Phe Val Lys Ser Ala Gly Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Thr Met Ser Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Pro
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Ser Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Ile Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro Gln
725 730 735
Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg Thr
740 745 750
Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Gly Ala Ile Pro
755 760 765
Phe Ser Asn Asn Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu Gly
770 775 780
Ser Met Pro Lys Ser Ser Asn Ser Val Tyr Ile Asn Gly Ser Thr His
785 790 795 800
His Asp Ser Asn Ile Asp Ile Phe Arg Gly Met Gln Ile Gly Pro His
805 810 815
Ile Lys Ser Asp Leu Val Ser Ser Glu Asn Asp Val Leu Gly Lys Gln
820 825 830
Phe Val Arg Ser Pro His Val Glu Pro Tyr Asp Phe Gln Ser Val Leu
835 840 845
Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile Asn
850 855 860
Pro Ser Phe Asp Pro Cys Pro Ser Pro Ser Leu Leu Asn Arg Thr Asn
865 870 875 880
Gly Leu
<210> 7
<211> 898
<212> PRT
<213> 豌豆(Pisum sativum)
<400> 7
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Arg Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Arg Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ala Lys Ser Arg Leu
100 105 110
Glu Lys Ser Ser Lys Ser Leu Leu Phe Leu Pro Leu Pro Lys Pro Ala
115 120 125
Cys Ile Arg Cys Gly Ser Asn Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Thr Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Met Leu Lys Asp Gln Pro
180 185 190
Ser Ala Val Ala Gln Leu Asn Ser Arg Glu Val Lys Thr Pro Thr Asn
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Arg Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Ala Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Trp Val Asp Glu Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Glu
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Met Phe Ser Asp Asp
435 440 445
Ala Lys Ser Met Glu Ser Ala Lys Gln Leu Met Gln Glu Ile His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Lys Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Glu His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Ser Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Thr Asp Lys Leu Leu Ile His Ser Ser Arg Val Leu
705 710 715 720
Lys Ser Asn Pro Asn Glu Ser Glu Asn His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
His Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Gly Ser Gly Ala
770 775 780
Ile Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys
785 790 795 800
Leu Gly Ser Met Pro Lys Ser Ser Asn Tyr Ile Asn Ser Ser Ser His
805 810 815
His Asp Ser Asn Ile Asp Ile Phe Arg Gly Met Gln Ile Gly Ser His
820 825 830
Ile Lys Ser Glu Leu Val Ser Ser Glu Asn Asp Ala Leu Thr Lys Gln
835 840 845
Phe Val Arg Ser Pro His Pro Glu Pro Tyr Asp Phe Gln Ser Val Leu
850 855 860
Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile Asn
865 870 875 880
Pro Ser Phe Asp Pro Asn Pro Ser Ser Ser Leu Leu Asn Arg Thr Asn
885 890 895
Gly Leu
<210> 8
<211> 887
<212> PRT
<213> 大豆(Glycine max)
<400> 8
Met Leu Thr Trp Trp Gly Lys Ser Ser Ser Lys Lys Thr Lys Lys Lys
1 5 10 15
Ala Asn Lys Glu Ser Phe Phe Asp Thr Leu His Arg Lys Leu Arg Ile
20 25 30
Ser Ser Glu Gly Lys Val Asn Ile Arg Ser Gly Gly Ser Arg Arg His
35 40 45
Cys Asn Asp Thr Ile Ser Glu Lys Gly Asp His Ser Pro Ser Gly Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ile Asp
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser Arg Leu
100 105 110
Glu Lys Val Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Thr Pro Gly
115 120 125
Cys Ile Arg Cys Arg Pro Asn Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Thr Pro Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser His Asn Arg Ser Pro Leu Ala Thr Asp Cys Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Ser Met Leu Lys Asp Gln Pro
180 185 190
Pro Thr Val Ser Leu Leu Asn Ser Thr Gly Val Lys Lys Pro Gly Asn
195 200 205
Ile Leu Ser Asn His Met Ser Ser Thr Ser Pro Lys His Arg Pro Leu
210 215 220
Arg Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Ala Phe Tyr
225 230 235 240
Ser Thr Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Leu Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Val Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly Tyr Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Leu Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Lys Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr His Arg Leu Pro Leu Pro Pro Leu Ser Val Ser Asn
355 360 365
Ser Ser Pro Phe Ser His Ser Asn Ser Ala Ala Thr Ser Pro Ser Met
370 375 380
Pro Arg Ser Pro Ala Arg Ala Asp Asn Pro Ser Ser Gly Ser Arg Trp
385 390 395 400
Lys Lys Gly Lys Leu Leu Gly Ser Gly Ser Phe Gly His Val Tyr Leu
405 410 415
Gly Phe Asn Ser Glu Ser Gly Glu Met Cys Ala Val Lys Glu Val Thr
420 425 430
Leu Phe Ser Asp Asp Pro Lys Ser Met Glu Ser Ala Lys Gln Phe Met
435 440 445
Gln Glu Ile His Leu Leu Ser Arg Leu Gln His Pro Asn Ile Val Gln
450 455 460
Tyr Tyr Gly Ser Glu Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu
465 470 475 480
Tyr Val Ser Gly Gly Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln
485 490 495
Phe Gly Glu Leu Val Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly
500 505 510
Leu Ala Tyr Leu His Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly
515 520 525
Ala Asn Ile Leu Val Asp Pro Thr Gly Arg Val Lys Leu Ala Asp Phe
530 535 540
Gly Met Ala Lys His Ile Thr Gly Gln Ser Cys Leu Leu Ser Phe Lys
545 550 555 560
Gly Thr Pro Tyr Trp Met Ala Pro Glu Val Ile Lys Asn Ser Asn Gly
565 570 575
Cys Asn Leu Ala Val Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu
580 585 590
Met Ala Thr Thr Lys Pro Pro Trp Phe Gln Tyr Glu Ala Val Ala Ala
595 600 605
Met Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His
610 615 620
Leu Ser Asn Glu Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn
625 630 635 640
Pro Tyr Asp Arg Pro Ser Ala Cys Glu Leu Leu Asp His Pro Phe Val
645 650 655
Lys Asn Ala Ala Pro Leu Glu Arg Pro Ile Leu Ala Pro Glu Val Leu
660 665 670
Asp Pro Val Ser Gly Ile Ile Gln Gly Ala Lys Ala Leu Ala Ala Gly
675 680 685
Gln Gly Lys Asn Leu Ser Ser Leu Asp Ser Asp Arg Leu Ser Ile His
690 695 700
Ser Ser Arg Phe Leu Lys Thr Asn Pro Arg Glu Ser Glu Ile His Ile
705 710 715 720
Pro Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu
725 730 735
Arg Ser Arg Ser Pro Gln His Arg Asn Gly Lys Met Ser Pro Pro Ile
740 745 750
Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Ala Gly Gly
755 760 765
Ser Gly Ala Ile Pro Phe Gly Asn His Ser Lys Gln Pro Ile Tyr Phe
770 775 780
Gln Glu Gly Phe Gly Ser Ile Pro Lys Ser Ser Asn Gly Val Tyr Ile
785 790 795 800
Asn Gly His Ser His His Asp Ser Ser Val Asp Ile Phe Arg Gly Met
805 810 815
Gln Ile Gly Ser His Ile Gln Pro Glu Leu Val Ser Ser Glu Asn Asp
820 825 830
Val Leu Val Asn Gln Phe Ala Arg His Pro His Ala Glu Pro Tyr Asp
835 840 845
Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Arg Glu
850 855 860
His Val Lys Ile Asn Pro Ser Ile Asp Leu Ser Pro Asn Ser Ser Leu
865 870 875 880
Leu Ser Arg Pro Asn Gly Leu
885
<210> 9
<211> 888
<212> PRT
<213> 大豆(Glycine max)
<400> 9
Met Leu Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Asn Lys Glu Ser Phe Phe Asp Thr Leu His Arg Lys Leu Arg Ile
20 25 30
Ser Ser Lys Gly Lys Val Ser Ile Arg Ser Gly Gly Ser Arg Arg His
35 40 45
Cys Asn Asp Thr Ile Ser Glu Lys Gly Asp His Ser Pro Cys Gly Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ile Asp
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser Arg Leu
100 105 110
Glu Lys Val Ser Lys Pro Leu Ser Phe Leu Thr Leu Pro Thr Pro Gly
115 120 125
Cys Ile Arg Cys Arg Pro Asn Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Thr Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser His Asn Arg Ser Pro Leu Ala Ile Asp Cys Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Met Leu Lys Asp Gln Pro
180 185 190
Pro Ala Val Ser Gln Leu Asn Ser Thr Gly Val Lys Lys Pro Gly Asn
195 200 205
Ile Leu Ser Asn His Met Ser Ser Thr Ser Pro Lys Arg Arg Pro Leu
210 215 220
Arg Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Ala Phe Tyr
225 230 235 240
Ser Ala Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Leu Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Leu Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Lys Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr His Arg Leu Pro Leu Pro Pro Leu Ser Val Ser Asn
355 360 365
Ser Ser Leu Phe Ser His Ser Asn Ser Ala Ala Thr Ser Pro Ser Met
370 375 380
Pro Arg Ser Pro Ala Arg Ala Asp Asn Pro Asn Ser Gly Ser Arg Trp
385 390 395 400
Lys Lys Gly Lys Leu Leu Gly Ser Gly Ser Phe Gly His Val Tyr Leu
405 410 415
Gly Phe Asn Ser Glu Arg Gly Glu Met Cys Ala Val Lys Glu Val Thr
420 425 430
Leu Phe Ser Asp Asp Pro Lys Ser Met Glu Ser Ala Lys Gln Phe Met
435 440 445
Gln Glu Ile His Leu Leu Ser Arg Leu Gln His Pro Asn Ile Val Gln
450 455 460
Tyr Tyr Gly Ser Glu Thr Val Asp Asn Lys Leu Tyr Ile Tyr Leu Glu
465 470 475 480
Tyr Val Ser Gly Gly Ser Ile His Lys Leu Leu Arg Glu Tyr Gly Gln
485 490 495
Phe Gly Glu Leu Val Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly
500 505 510
Leu Ala Tyr Leu His Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly
515 520 525
Ala Asn Ile Leu Val Asp Pro Thr Gly Arg Val Lys Leu Ala Asp Phe
530 535 540
Gly Met Ala Lys His Ile Thr Gly Gln Ser Cys Pro Leu Ser Phe Lys
545 550 555 560
Gly Thr Pro Tyr Trp Met Ala Pro Glu Val Ile Lys Asn Ser Asn Gly
565 570 575
Cys Asn Leu Ala Val Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu
580 585 590
Met Ala Thr Thr Lys Pro Pro Trp Phe Gln Tyr Glu Gly Val Ala Ala
595 600 605
Met Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His
610 615 620
Leu Ser Asn Glu Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn
625 630 635 640
Pro His Asp Arg Pro Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val
645 650 655
Lys Asn Ala Ala Pro Leu Glu Arg Pro Ile Pro Ala Pro Glu Ala Leu
660 665 670
Asp Pro Val Ser Gly Ile Thr Gln Gly Ala Lys Ala Leu Ala Ile Gly
675 680 685
Gln Gly Arg Asn Leu Ser Ser Leu Asp Ser Asp Arg Leu Ser Val His
690 695 700
Ser Ser Arg Phe Leu Lys Thr Asn Pro His Glu Ser Glu Ile His Ile
705 710 715 720
Pro Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu
725 730 735
Arg Ser Arg Ser Pro Gln His Arg Asn Gly Lys Met Ser Pro Ser Pro
740 745 750
Ile Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Ala Gly
755 760 765
Gly Ser Gly Ala Ile Pro Phe Gly Asn His Ser Lys Gln Ser Ile Tyr
770 775 780
Phe Gln Glu Gly Phe Gly Ser Ile Pro Lys Ser Ser Asn Gly Val Tyr
785 790 795 800
Met Asn Gly His Ser His His Asp Ser Asn Val Asp Ile Phe Arg Gly
805 810 815
Met Gln Met Gly Ser His Ile Ser Pro Glu Leu Val Ser Ser Glu Asn
820 825 830
Asp Val Leu Val Lys Gln Phe Ala Arg His Pro His Ala Glu Pro Tyr
835 840 845
Asp Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly
850 855 860
Glu His Val Lys Ile Asn Pro Ser Ile Asp Leu Ser Pro Asn Ser Ser
865 870 875 880
Leu Leu Ser Arg Pro Asn Gly Leu
885
<210> 10
<211> 823
<212> PRT
<213> 菜豆(Phaseolus vulgaris)
<400> 10
Gln Ser Phe Ile Glu Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly
1 5 10 15
Leu His Pro Ser Ser Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser
20 25 30
Ser Lys Ser Arg Leu Glu Lys Gly Ser Lys Pro Ser Leu Phe Leu Pro
35 40 45
Leu Pro Thr Pro Gly Cys Ile Arg Cys Lys Pro Asn Pro Ala Asp Leu
50 55 60
Asp Gly Asp Met Val Thr Ala Ser Val Phe Ser Asp Cys Ser Ala Asp
65 70 75 80
Ser Asp Glu Pro His Ser His Asn Arg Ser Pro Leu Val Ala Asp Cys
85 90 95
Glu Thr Gly Ser Arg Thr Ala Ala Ala Gly Ser Pro Ser Ser Thr Met
100 105 110
Leu Lys Asp Gln Pro Ile Val Val Ser Gln Leu Asn Ser Ala Gly Val
115 120 125
Lys Lys Pro Gly Ser Ile Leu Ser Asn His Thr Ser Ser Thr Ser Pro
130 135 140
Lys Arg Arg Pro Leu Arg Asn His Val Pro Asn Leu Gln Val Pro Pro
145 150 155 160
His Gly Ala Phe Tyr Ser Ala Pro Asp Ser Ser Leu Ser Ser Pro Ser
165 170 175
Arg Ser Pro Leu Arg Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala
180 185 190
Phe Trp Ser Gly Lys Pro Tyr Ser Glu Val Asn Leu Val Gly Ser Gly
195 200 205
Gln Cys Ser Ser Pro Gly Ser Gly His Asn Ser Gly His Asn Ser Met
210 215 220
Gly Gly Asp Met Ser Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser
225 230 235 240
Pro Glu Tyr Ser Pro Val Pro Ser Pro Arg Met Ala Ser Pro Gly Pro
245 250 255
Ser Ser Arg Ile Gln Ser Gly Ala Val Thr Pro Ile His Pro Lys Ala
260 265 270
Gly Gly Thr Pro Ile Glu Ser Gln Thr Gly Arg Val Asp Asp Gly Lys
275 280 285
Gln Gln Thr His Arg Leu Pro Leu Pro Pro Leu Ser Val Ser Asn Ser
290 295 300
Ser Pro Phe Ser His Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro
305 310 315 320
Arg Ser Pro Ala Arg Ala Asp Thr Pro Gly Ser Gly Ser Arg Trp Lys
325 330 335
Lys Gly Lys Leu Leu Gly Ser Gly Ser Phe Gly His Val Tyr Leu Gly
340 345 350
Phe Asn Ser Glu Ser Gly Glu Met Cys Ala Val Lys Glu Val Thr Leu
355 360 365
Phe Ser Asp Asp Pro Lys Ser Met Glu Ser Ala Lys Gln Phe Met Gln
370 375 380
Glu Ile His Leu Leu Ser Arg Leu Gln His Pro Asn Ile Val Gln Tyr
385 390 395 400
Tyr Gly Ser Glu Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr
405 410 415
Val Ser Gly Gly Ser Ile His Lys Leu Leu Arg Glu Tyr Gly Gln Phe
420 425 430
Gly Glu Leu Val Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu
435 440 445
Ala Tyr Leu His Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala
450 455 460
Asn Ile Leu Val Asp Pro Thr Gly Arg Val Arg Leu Ala Asp Phe Gly
465 470 475 480
Met Ala Lys His Ile Thr Gly Gln Ser Cys Pro Leu Ser Phe Lys Gly
485 490 495
Thr Pro Tyr Trp Met Ala Pro Glu Val Ile Lys Asn Ser Asn Gly Cys
500 505 510
Asn Leu Ala Val Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met
515 520 525
Ala Thr Thr Lys Pro Pro Trp Phe Gln Tyr Glu Gly Val Ala Ala Met
530 535 540
Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu
545 550 555 560
Ser Asn Glu Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro
565 570 575
Tyr Asp Arg Pro Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val Lys
580 585 590
Leu Ala Ala Pro Leu Glu Arg Pro Ile Leu Val Thr Glu Ala Leu Asp
595 600 605
His Val Ser Gly Thr Thr Gln Gly Ala Lys Ala Leu Ala Ile Gly Gln
610 615 620
Ala Lys Asn Leu Ser Thr Leu Asp Ser Asp Arg Leu Ser Val His Ser
625 630 635 640
Ser Arg Phe Leu Lys Ile Asn Pro His Glu Ser Glu Ile His Ile Pro
645 650 655
Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg
660 665 670
Ser Arg Ser Pro Gln His Arg Asn Gly Arg Met Ser Pro Ser Pro Ile
675 680 685
Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Ala Gly Gly
690 695 700
Ser Gly Ala Ile Pro Phe Gly Asn His Ser Lys Gln Leu Ile Tyr Phe
705 710 715 720
Gln Glu Gly Phe Gly Ser Ile Pro Lys Ser Ser Asn Gly Met Tyr Val
725 730 735
Asn Gly Pro Ala His His Asp Ser Thr Val Asp Ile Phe Arg Gly Met
740 745 750
Gln Ile Gly Ser His Ile Pro Ser Glu Leu Val Ser Ser Glu Asn Asp
755 760 765
Val Leu Val Lys Gln Phe Ala Arg His Pro His Ala Glu Pro His Asp
770 775 780
Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Glu
785 790 795 800
His Val Arg Ile Asn Pro Ser Leu Asp Leu Ser Pro Ser Ser Ser Leu
805 810 815
Leu Ser Arg Pro Asn Gly Leu
820
<210> 11
<211> 846
<212> PRT
<213> 赤豆(Vigna angularis)
<400> 11
Met Leu Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Ser Lys Lys Lys
1 5 10 15
Ser Asn Lys Glu Ser Phe Phe Asp Thr Leu His Arg Lys Leu Lys Ile
20 25 30
Ser Ser Glu Gly Lys Leu Ser Thr Arg Ser Gly Gly Ser Arg Arg Asn
35 40 45
Cys Asn Asp Thr Val Ser Glu Lys Gly Asp Arg Ser Pro Thr Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ile Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser Arg Leu
100 105 110
Glu Lys Val Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Thr Pro Gly
115 120 125
Cys Ile Arg Cys Lys Pro Asn Pro Ala Asp Leu Asp Gly Asp Leu Val
130 135 140
Thr Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro His
145 150 155 160
Ser His Asn Arg Ser Pro Leu Val Ala Asp Cys Glu Ile Gly Ile Arg
165 170 175
Thr Ala Ala Gly Ser Pro Ser Arg Pro Leu Arg Asn His Val Pro Asn
180 185 190
Leu Gln Val Pro Pro His Gly Ala Phe Tyr Ser Ala Pro Asp Ser Ser
195 200 205
Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg Ala Phe Gly Thr Asp Gln
210 215 220
Val Leu Asn Ser Ala Phe Trp Ala Gly Lys Pro Tyr Ser Glu Val Asn
225 230 235 240
Phe Val Gly Ser Gly His Cys Ser Ser Pro Gly Ser Gly His Asn Ser
245 250 255
Gly His Asn Ser Met Gly Gly Asp Met Ser Gly Pro Leu Phe Trp Gln
260 265 270
Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro Val Pro Ser Pro Arg Met
275 280 285
Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln Ser Gly Ala Val Thr Pro
290 295 300
Ile His Pro Lys Ala Gly Gly Thr Pro Ile Glu Ser Gln Thr Gly Arg
305 310 315 320
Val Asp Asp Gly Lys Gln Gln Thr His Arg Leu Pro Leu Pro Pro Leu
325 330 335
Ser Val Ser Asn Ser Ser Pro Phe Ser His Ser Asn Ser Ala Ala Thr
340 345 350
Ser Pro Ser Met Pro Arg Ser Pro Ala Arg Ala Asp Asn Pro Gly Ser
355 360 365
Gly Ser Arg Trp Lys Lys Gly Lys Leu Leu Gly Ser Gly Ser Phe Gly
370 375 380
His Val Tyr Leu Gly Phe Asn Ser Glu Ser Gly Glu Met Cys Ala Val
385 390 395 400
Lys Glu Val Thr Leu Phe Ser Asp Asp Pro Lys Ser Met Glu Ser Ala
405 410 415
Lys Gln Phe Met Gln Glu Ile His Leu Leu Ser Arg Leu Gln His Pro
420 425 430
Asn Ile Val Arg Tyr Tyr Gly Ser Glu Thr Val Asp Asp Lys Leu Tyr
435 440 445
Ile Tyr Leu Glu Tyr Val Ser Gly Gly Ser Ile His Lys Leu Leu Arg
450 455 460
Glu Tyr Gly Gln Phe Gly Glu Leu Val Ile Arg Ser Tyr Thr Gln Gln
465 470 475 480
Ile Leu Ser Gly Leu Ala Tyr Leu His Ala Lys Asn Thr Leu His Arg
485 490 495
Asp Ile Lys Gly Ala Asn Ile Leu Val Asp Pro Thr Gly Arg Val Lys
500 505 510
Leu Ala Asp Phe Gly Met Ala Lys His Ile Thr Gly Gln Ser Cys Pro
515 520 525
Leu Ser Phe Lys Gly Thr Pro Tyr Trp Met Ala Pro Glu Val Ala Ala
530 535 540
Met Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His
545 550 555 560
Leu Ser Asn Glu Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn
565 570 575
Pro Tyr Asp Arg Pro Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val
580 585 590
Lys Leu Ala Ala Pro Leu Glu Arg Pro Ile Leu Val Pro Glu Ala Leu
595 600 605
Asp Pro Val Ser Gly Thr Thr Gln Gly Ala Lys Ser Leu Val Phe Glu
610 615 620
Arg Pro Arg Leu Asn Leu Ser Val Val Leu Pro Lys Pro Lys Thr His
625 630 635 640
Pro Asn Ser Pro Leu Phe Ser Ser Ser Tyr Leu Ile Val Cys Cys His
645 650 655
Gly Asn Cys Ser Cys Met Arg Gln His Glu Trp Cys Arg Arg Lys Arg
660 665 670
Gln Gly Asp Glu Ile His Ile Pro Lys Asn Ile Ser Cys Pro Val Ser
675 680 685
Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro Gln His Arg Asn
690 695 700
Gly Lys Met Ser Pro Pro Ile Ser Ser Pro Arg Thr Ala Ser Gly Ala
705 710 715 720
Ser Thr Pro Leu Ala Gly Gly Ser Gly Ala Ile Pro Phe Gly Asn His
725 730 735
Ser Lys Gln Leu Ile Tyr Phe Gln Glu Ser Phe Gly Ser Ile Pro Lys
740 745 750
Ser Ser Asn Gly Val Tyr Val Asn Gly Pro Ala His His Asp Ser Thr
755 760 765
Val Asp Ile Phe Arg Gly Met Gln Ile Gly Ser His Ile Pro Ser Glu
770 775 780
Leu Val Ser Ser Glu Asn Asp Val Leu Val Lys Gln Phe Ala Arg Pro
785 790 795 800
Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val Leu Ala Asp Arg Val
805 810 815
Gly Arg Gln Leu Leu Arg Glu His Val Lys Ile Asn Pro Ser Leu Asp
820 825 830
Leu Ser Pro Asn Ser Ser Leu Leu Ser Arg Pro Asn Gly Leu
835 840 845
<210> 12
<211> 871
<212> PRT
<213> 落花生(Arachis hypogaea)
<400> 12
Met Pro Ile Arg Trp Ala Asn Pro Phe Ser Asn Ser Lys Glu Thr Lys
1 5 10 15
Lys Lys Ala Ser Lys Glu Ser Phe Ile Gly Thr Leu His Arg Lys Phe
20 25 30
Lys Thr Pro Ser Glu Gly Arg Leu Gly Ser Arg Ser Gly Gly Pro Gly
35 40 45
Arg Arg Cys Asn Asp Thr Ile Ser Glu Met Gly Asp Arg Ser Pro Ala
50 55 60
Glu Ser Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe
65 70 75 80
Ala Glu Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro
85 90 95
Ser Ser Ile Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser
100 105 110
Arg Leu Glu Lys Ser Ser Lys Pro Ser Phe Leu Pro Leu Pro Arg Pro
115 120 125
Ala Cys Met Arg Gly Arg Thr Asn Pro Ser Asp Leu Asp Gly Asp Met
130 135 140
Val Ala Ser Ile Ser Ser Asp Gly Ser Ala Asp Ser Asp Asp Gln Thr
145 150 155 160
Glu Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Thr Asp Thr Gly Thr
165 170 175
Arg Thr Ala Ala Glu Ser Pro Ser Ser Met Met Leu Lys Asp Gln Pro
180 185 190
Ser Val Val Ser Gln Leu Asn Ser Arg Glu Ala Lys Lys Pro Val Asn
195 200 205
Ile Leu Ser Asn His Met Ser Ser Thr Ser Pro Lys Gln Arg Pro Leu
210 215 220
Arg Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Ala Phe Tyr
225 230 235 240
Ser Ala Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Ser Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Phe Asn Leu Ala Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Gln Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Ser Trp Ala Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Ile Thr Asn Ser Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Glu Asn Pro Val Ser Pro Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Ile Gly Arg Gly Thr Phe Gly His Val Tyr Val Gly Phe Asn Asn Asp
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Ile Ser Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile Tyr Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Asn Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Val His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Leu Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Lys Gly Gln Ser Cys Pro Leu Ser Ile Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Lys Asn Thr Ser Gly Cys Asn Leu Ala Val Asp
580 585 590
Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys Pro
595 600 605
Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly Asn
610 615 620
Ser Lys Glu Leu Pro Lys Ile Pro Asp His Leu Ser Asn Glu Gly Lys
625 630 635 640
Asp Phe Val Arg Arg Cys Leu Gln Arg Asp Pro His Asp Arg Pro Ser
645 650 655
Ala Ser Glu Leu Leu Glu His Ala Phe Val Lys Cys Ala Ala Pro Leu
660 665 670
Glu Arg Pro Ile Leu Gly Pro Glu Ala Ser Asp Pro His Pro Ala Ile
675 680 685
Thr Gln Gly Thr Lys Thr Leu Ile Asp Leu Leu Asp Ile His Ile Pro
690 695 700
Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg
705 710 715 720
Ser Arg Ser Pro Gln His Met Asn Gly Arg Met Ser Pro Ser Pro Ile
725 730 735
Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly
740 745 750
Asn Gly Ala Ile Pro Phe Ser Asn Gln Leu Lys Gln Ser Val Tyr Phe
755 760 765
Gln Glu Gly Leu Val Ser Met Pro Lys Ser Pro Asn Gly Val Tyr Ile
770 775 780
Ser Gly Ser Ala His Asn Asp Ser Asn Val Asp Ile Phe Arg Gly Met
785 790 795 800
Gln Met Gly Ser His Ile Thr Ser Glu Leu Val Pro Ser Glu Asn Asp
805 810 815
Ile Leu Gly Lys Gln Phe Thr Arg Pro Ser His Pro Glu Pro Tyr Asp
820 825 830
Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Arg Asp
835 840 845
His Val Lys Ile Asn Pro Ser Leu Asp Leu Asn Pro Asn Ser Thr Leu
850 855 860
Leu Ser Arg Gly Asn Gly Leu
865 870
<210> 13
<211> 872
<212> PRT
<213> 蔓花生(Arachis duranensis)
<400> 13
Met Pro Ile Arg Trp Ala Asn Pro Phe Ser Asn Ser Lys Glu Thr Lys
1 5 10 15
Lys Lys Ala Ser Lys Glu Ser Phe Ile Gly Thr Leu His Arg Lys Phe
20 25 30
Lys Thr Pro Ser Glu Gly Arg Leu Gly Ser Arg Ser Gly Gly Pro Gly
35 40 45
Arg Arg Cys Asn Asp Thr Ile Ser Glu Met Gly Asp Arg Ser Pro Ala
50 55 60
Glu Ser Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe
65 70 75 80
Ala Glu Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro
85 90 95
Ser Ser Ile Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser
100 105 110
Arg Leu Glu Lys Ser Ser Lys Pro Ser Phe Leu Pro Leu Pro Arg Pro
115 120 125
Ala Cys Met Arg Gly Arg Thr Asn Pro Ser Asp Leu Asp Gly Asp Met
130 135 140
Val Ala Ser Ile Ser Ser Asp Gly Ser Ala Asp Ser Asp Asp Gln Thr
145 150 155 160
Glu Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Thr Asp Thr Gly Thr
165 170 175
Arg Thr Ala Ala Glu Ser Pro Ser Ser Met Met Leu Lys Asp Gln Pro
180 185 190
Ser Val Val Ser Gln Leu Asn Ser Arg Glu Ala Lys Lys Pro Val Asn
195 200 205
Ile Leu Ser Asn His Met Ser Ser Thr Ser Pro Lys Gln Arg Pro Leu
210 215 220
Arg Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Ala Phe Tyr
225 230 235 240
Ser Ala Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Ser Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Phe Asn Leu Ala Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Gln Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Val Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Ser Trp Ala Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Ile Thr Asn Ser Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Glu Asn Pro Val Ser Pro Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Ile Gly Arg Gly Thr Phe Gly His Val Tyr Val Gly Phe Asn Asn Asp
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Ile Ser Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile Tyr Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Asn Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Val His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Leu Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Lys Gly Gln Ser Cys Pro Leu Ser Ile Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Thr Ser Gly Cys Asn Leu Ala Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Lys Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Arg Cys Leu Gln Arg Asp Pro His Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Glu His Ala Phe Val Lys Cys Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Leu Gly Pro Asp Ala Ser Asp Pro His Pro Ala
675 680 685
Ile Thr Gln Gly Thr Lys Thr Leu Ile Asp Leu Leu Asp Ile His Ile
690 695 700
Pro Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu
705 710 715 720
Arg Ser Arg Ser Pro Gln His Met Asn Gly Arg Met Ser Pro Ser Pro
725 730 735
Ile Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly
740 745 750
Gly Asn Gly Ala Ile Pro Phe Ser Asn Gln Leu Lys Gln Ser Val Tyr
755 760 765
Phe Gln Glu Gly Leu Val Ser Met Pro Lys Ser Pro Asn Gly Val Tyr
770 775 780
Ile Ser Gly Ser Ala His Asn Asp Ser Asn Val Asp Ile Phe Arg Gly
785 790 795 800
Met Gln Met Gly Ser His Ile Thr Ser Glu Leu Val Pro Ser Glu Asn
805 810 815
Asp Ile Leu Gly Lys Gln Phe Thr Arg Pro Ser His Pro Glu Pro Tyr
820 825 830
Asp Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Arg
835 840 845
Asp His Val Lys Ile Asn Pro Ser Leu Asp Leu Asn Pro Asn Ser Thr
850 855 860
Leu Leu Ser Arg Gly Asn Gly Leu
865 870
<210> 14
<211> 911
<212> PRT
<213> 绿豆(Vigna radiata)
<400> 14
Met Leu Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Ser Lys Lys Lys
1 5 10 15
Ser Asn Lys Glu Ser Phe Phe Asp Thr Leu His Arg Lys Leu Lys Ile
20 25 30
Ser Ser Glu Gly Lys Leu Ser Thr Arg Ser Gly Gly Ser Arg Arg Asn
35 40 45
Cys Asn Asp Thr Val Ser Glu Lys Gly Asp Arg Ser Pro Thr Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Gly Arg Cys Gln Ser Phe Ile Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Val Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ser Lys Ser Arg Leu
100 105 110
Glu Lys Val Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Thr Pro Gly
115 120 125
Cys Ile Arg Cys Lys Pro Asn Pro Ala Asp Leu Asp Gly Asp Met Ile
130 135 140
Thr Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Leu His
145 150 155 160
Ser His Asn Arg Ser Pro Leu Val Ala Asp Cys Glu Thr Gly Ile Arg
165 170 175
Thr Ala Ala Gly Ser Pro Ser Ser Ser Met Pro Lys Asp Gln Pro Val
180 185 190
Val Ala Ser Gln Leu Asn Ser Ala Gly Val Lys Lys Pro Gly Ser Ile
195 200 205
Leu Ser Asn His Thr Ser Ser Thr Ser Pro Lys Arg Arg Pro Leu Arg
210 215 220
Asn His Val Pro Asn Leu Gln Val Pro Pro His Gly Ala Phe Tyr Ser
225 230 235 240
Ala Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg Ala
245 250 255
Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys Pro
260 265 270
Tyr Ser Glu Val Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro Gly
275 280 285
Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser Gly
290 295 300
Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro Val
305 310 315 320
Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln Ser
325 330 335
Gly Ala Val Thr Pro Ile His Pro Lys Ala Gly Gly Thr Pro Ile Glu
340 345 350
Ser Gln Thr Gly Arg Phe Asp Asp Gly Lys Gln Gln Thr His Arg Leu
355 360 365
Pro Leu Pro Pro Leu Ser Val Ser Asn Ser Ser Pro Phe Ser His Ser
370 375 380
Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg Ala
385 390 395 400
Asp Asn Pro Gly Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu Leu Gly
405 410 415
Ser Gly Ser Phe Gly His Val Tyr Leu Gly Phe Asn Ser Glu Ser Gly
420 425 430
Glu Met Cys Ala Val Lys Glu Val Thr Leu Phe Ser Asp Asp Pro Lys
435 440 445
Ser Met Glu Ser Ala Lys Gln Phe Met Gln Asn Pro Phe Lys Asn Tyr
450 455 460
Trp Gln Glu Ile His Leu Leu Ser Arg Leu Gln His Pro Asn Ile Val
465 470 475 480
Arg Tyr Tyr Gly Ser Glu Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu
485 490 495
Glu Tyr Val Ser Gly Gly Ser Ile His Lys Leu Leu Arg Glu Tyr Gly
500 505 510
Gln Phe Gly Glu Leu Val Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser
515 520 525
Gly Leu Ala Tyr Leu His Ala Lys Asn Thr Leu His Arg Asp Ile Lys
530 535 540
Gly Ala Asn Ile Leu Val Asp Pro Thr Gly Arg Val Lys Leu Ala Asp
545 550 555 560
Phe Gly Met Ala Lys His Ile Thr Gly Gln Ser Cys Pro Leu Ser Phe
565 570 575
Lys Gly Thr Pro Tyr Trp Met Ala Pro Glu Val Ile Lys Asn Ser Asn
580 585 590
Gly Cys Asn Leu Ala Val Asp Ile Trp Ser Leu Gly Cys Thr Val Leu
595 600 605
Glu Met Ala Thr Thr Lys Pro Pro Trp Phe Gln Tyr Glu Gly Val Ala
610 615 620
Ala Met Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp
625 630 635 640
His Leu Ser Asn Glu Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg
645 650 655
Asn Pro Tyr Asp Arg Pro Ser Ala Ser Glu Leu Leu Asp His Pro Phe
660 665 670
Val Lys Leu Ala Ala Pro Leu Glu Arg Pro Ile Leu Val Pro Glu Ala
675 680 685
Met Asp Pro Val Ser Gly Thr Thr Gln Gly Ala Lys Pro Leu Met His
690 695 700
Val Met Pro Met His Val Met Pro Met His Val Met Pro Met His Val
705 710 715 720
Met Pro Met His Val Met Pro Met His Val Met Ser Met His Val Met
725 730 735
Leu His Val Glu Ile His Ile Pro Lys Asn Ile Ser Cys Pro Val Ser
740 745 750
Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro Gln His Arg Asn
755 760 765
Gly Lys Met Ser Pro Ser Pro Ile Ser Ser Pro Arg Thr Ala Ser Gly
770 775 780
Ala Ser Thr Pro Leu Ala Gly Gly Ser Gly Ala Ile Pro Phe Gly Asn
785 790 795 800
His Ser Lys Gln Leu Ile Tyr Phe Gln Glu Ser Phe Gly Ser Ile Pro
805 810 815
Lys Ser Ser Asn Gly Val Tyr Val Asn Gly Pro Ala His His Asp Ser
820 825 830
Thr Val Asp Ile Phe Arg Gly Met Gln Ile Gly Ser His Ile Gln Ser
835 840 845
Glu Leu Val Ser Thr Glu Asn Asp Val Leu Val Lys Gln Phe Ala Arg
850 855 860
Pro Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val Leu Ala Asp Arg
865 870 875 880
Val Gly Arg Gln Leu Leu Arg Glu His Val Lys Ile Asn Pro Ser Leu
885 890 895
Asp Leu Ser Pro Asn Ser Ser Leu Leu Ser Arg Pro Asn Gly Leu
900 905 910
<210> 15
<211> 712
<212> PRT
<213> 车轴草(Trifolium pratense)
<400> 15
Met Leu Lys Asp Gln Pro Ser Ala Val Ser Gln Val Asn Ser Arg Glu
1 5 10 15
Val Lys Lys Ser Ala Asn Ile Leu Ser Asn Gln Thr Pro Ser Thr Ser
20 25 30
Pro Lys Arg Arg Pro Leu Arg His His Val Pro Asn Leu Gln Val Pro
35 40 45
Pro His Gly Ile Phe Tyr Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro
50 55 60
Ser Arg Ser Pro Leu Arg Ala Phe Gly Thr Asp Gln Val Leu Asn Ser
65 70 75 80
Ala Phe Trp Ala Gly Lys Gln Tyr Pro Glu Val Asn Phe Ile Gly Ser
85 90 95
Gly His Cys Ser Ser Pro Gly Ser Gly His Asn Ser Gly His Asn Ser
100 105 110
Met Gly Gly Asp Met Ser Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly
115 120 125
Ser Pro Glu Tyr Ser Pro Val Pro Ser Pro Arg Met Thr Ser Pro Gly
130 135 140
Pro Ser Ser Arg Ile Gln Ser Gly Ala Val Thr Pro Ile His Pro Arg
145 150 155 160
Ala Ala Gly Thr Pro Thr Glu Ser Gln Thr Gly Arg Val Asp Asp Gly
165 170 175
Lys Gln Gln Ser His Arg Leu Pro Leu Pro Pro Leu Thr Val Asn Asn
180 185 190
Thr Ser Pro Phe Ser His Ser Asn Ser Ala Ala Thr Ser Pro Ser Met
195 200 205
Pro Arg Ser Pro Ala Arg Ala Asp Ser Pro Met Ser Ser Gly Ser Arg
210 215 220
Trp Lys Lys Gly Lys Leu Leu Gly Arg Gly Thr Phe Gly His Val Tyr
225 230 235 240
Ile Gly Phe Asn Ser Glu Ser Gly Glu Met Cys Ala Met Lys Glu Val
245 250 255
Thr Met Phe Ser Asp Asp Ala Lys Ser Met Glu Ser Ala Lys Gln Leu
260 265 270
Met Gln Glu Ile His Leu Leu Ser Arg Leu Arg His Pro Asn Ile Val
275 280 285
Gln Tyr Tyr Gly Ser Glu Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu
290 295 300
Glu Tyr Val Ser Gly Gly Ser Ile His Lys Leu Leu Gln Glu Tyr Gly
305 310 315 320
Gln Phe Gly Glu Leu Ala Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser
325 330 335
Gly Leu Ala Phe Leu His Ala Lys Asn Thr Leu His Arg Asp Ile Lys
340 345 350
Gly Ala Asn Ile Leu Val Asp Pro Asn Gly Arg Val Lys Val Ala Asp
355 360 365
Phe Gly Met Ala Lys His Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe
370 375 380
Lys Gly Ser Pro Tyr Trp Met Ala Pro Glu Val Ile Lys Asn Ser Lys
385 390 395 400
Glu Cys Ser Leu Gly Val Asp Ile Trp Ser Leu Gly Cys Thr Val Leu
405 410 415
Glu Met Ala Thr Thr Lys Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala
420 425 430
Ala Met Phe Lys Ile Gly Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp
435 440 445
His Leu Ser Asp Ala Gly Lys Asp Phe Val Arg Lys Cys Leu Gln Arg
450 455 460
Asn Pro Arg Asp Arg Pro Ser Ala Ser Glu Leu Leu Asp His Pro Phe
465 470 475 480
Val Lys Gly Ala Ala Pro Leu Glu Arg Pro Ile Met Val Pro Glu Ala
485 490 495
Ser Asp Pro Val Ser Gly Ile Thr His Gly Thr Lys Ala Leu Gly Ile
500 505 510
Gly Gln Gly Arg Asn Leu Ser Ala Leu Asp Ser Asp Lys Leu Leu Val
515 520 525
His Ser Ser Arg Val Leu Lys Ser Asn Pro His Glu Ser Glu Asn His
530 535 540
Ile His Arg Asn Ile Ser Cys Pro Val Ser Pro Ile Gly Ser Pro Leu
545 550 555 560
Leu Arg Ser Arg Ser Pro His Gln Arg Ser Gly Arg Leu Ser Pro Ser
565 570 575
Pro Ile Ser Ser Pro Arg Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr
580 585 590
Gly Gly Ser Gly Ala Ile Pro Phe Asn His Leu Lys Gln Ala Ala Tyr
595 600 605
Phe Gln Glu Cys Leu Gly Ser Met Pro Lys Ser Ser Asn Gly Val Tyr
610 615 620
Met Asn Gly Ser Thr His His Asp Pro Asn Ile Asp Ile Phe Arg Gly
625 630 635 640
Met Gln Thr Gly Ser His Ile Lys Ser Glu Leu Val Ser Ser Glu Asn
645 650 655
Asp Ala Leu Ser Lys Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr
660 665 670
Asp Phe Gln Ser Val Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly
675 680 685
Asp His Val Lys Ile Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Ser
690 695 700
Met Leu His Arg Thr Asn Gly Leu
705 710
<210> 16
<211> 899
<212> PRT
<213> 紫花苜蓿(Medicago sativa)
<400> 16
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Ile Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Leu Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ala Lys Ser Arg Leu
100 105 110
Glu Lys Pro Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ser
115 120 125
Cys Ile Arg Cys Gly Ser Thr Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Val Leu Lys Asp Gln Ser
180 185 190
Ser Ala Val Ser Gln Pro Asn Ser Arg Glu Val Lys Lys Thr Ala Asn
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Lys Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Met Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Ile Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Arg Val Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Gln
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Val His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Glu His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Thr Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Leu Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Asn Asn Pro His Glu Ser Glu Ile His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
Gln Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Gly Ala Ile
770 775 780
Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu
785 790 795 800
Gly Ser Met Pro Lys Ser Pro Asn Gly Ala Tyr Ile Asn Gly Ser Ser
805 810 815
His His Asp Ser Asn Ile Asp Ile Phe Gln Arg Met Gln Ala Gly Ser
820 825 830
His Ile Lys Ser Glu Leu Val Ser Ser Asp Asn Asp Ala Leu Gly Lys
835 840 845
Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val
850 855 860
Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile
865 870 875 880
Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Ser Met Leu Asn Arg Thr
885 890 895
Asn Gly Leu
<210> 17
<211> 899
<212> PRT
<213> 紫花苜蓿(Medicago sativa)
<400> 17
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Ile Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Leu Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ala Lys Ser Arg Leu
100 105 110
Glu Lys Pro Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ser
115 120 125
Cys Ile Arg Cys Gly Pro Thr Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Val Leu Lys Asp Gln Ser
180 185 190
Thr Ala Val Ser Gln Pro Asn Ser Arg Glu Val Lys Lys Thr Glu Asn
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Lys Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Met Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Ile Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Arg Val Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Gln
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Val His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Asp Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Asp His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Ser Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Leu Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Asn Asn Pro Asn Glu Ser Glu Ile His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
Gln Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Ser Ala Ile
770 775 780
Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu
785 790 795 800
Gly Ser Met Pro Lys Ser Pro Asn Gly Val Tyr Ile Asn Gly Ser Ser
805 810 815
His His Asp Ser Asn Ile Asp Ile Phe Gln Arg Met Gln Ala Gly Ser
820 825 830
His Ile Lys Ser Glu Leu Val Ser Ser Asp Asn Asp Ala Leu Gly Lys
835 840 845
Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val
850 855 860
Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile
865 870 875 880
Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Ser Met Leu Asn Arg Thr
885 890 895
Asn Gly Leu
<210> 18
<211> 899
<212> PRT
<213> 紫花苜蓿(Medicago sativa)
<400> 18
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Ile Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Arg Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Leu Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Val Lys Ser Arg Leu
100 105 110
Glu Lys Pro Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ser
115 120 125
Cys Ile Arg Cys Gly Pro Thr Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Pro
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Val Leu Lys Asp Gln Ser
180 185 190
Ser Ala Val Ser Gln Pro Asn Ser Arg Glu Val Lys Lys Thr Ala Asn
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Lys Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Leu Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Ile Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Arg Val Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Gln
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Met Gln Glu Val His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Gly Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Glu His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Thr Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Leu Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Asn Asn Pro His Glu Ser Glu Ile His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
Gln Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Gly Ala Ile
770 775 780
Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu
785 790 795 800
Gly Ser Met Pro Lys Ser Pro Asn Gly Ala Tyr Ile Asn Gly Ser Ser
805 810 815
His His Asp Ser Asn Ile Asp Ile Phe Gln Arg Met Gln Ala Gly Ser
820 825 830
His Ile Lys Ser Glu Leu Val Ser Ser Asp Asn Asp Ala Leu Gly Lys
835 840 845
Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val
850 855 860
Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile
865 870 875 880
Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Thr Met Leu Asn Arg Thr
885 890 895
Asn Gly Leu
<210> 19
<211> 899
<212> PRT
<213> 紫花苜蓿(Medicago sativa)
<400> 19
Met Pro Thr Trp Trp Gly Lys Ser Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Gly Lys Glu Ser Ile Ile Asp Thr Leu His Arg Lys Phe Lys Phe
20 25 30
Pro Ser Glu Gly Lys Leu Ser Thr Ile Ser Gly Gly Ser Arg Arg Arg
35 40 45
Ser Asn Asp Thr Ile Ser Glu Lys Gly Asp Cys Ser Pro Ser Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro His Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Ser
85 90 95
Leu Gly Arg Val Asp Ser Glu Ile Ser Ile Ser Ala Lys Ser Arg Leu
100 105 110
Glu Lys Pro Ser Lys Pro Ser Leu Phe Leu Pro Leu Pro Lys Pro Ser
115 120 125
Cys Ile Arg Cys Gly Ser Thr Pro Ala Asp Leu Asp Gly Asp Met Val
130 135 140
Asn Ala Ser Val Phe Ser Asp Cys Ser Ala Asp Ser Asp Glu Pro Ala
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Val Leu Lys Asp Gln Ser
180 185 190
Ser Ala Val Ser Gln Pro Asn Ser Arg Glu Val Lys Lys Thr Ala Asn
195 200 205
Ile Leu Ser Asn His Thr Pro Ser Thr Ser Pro Lys Arg Lys Pro Leu
210 215 220
Arg His His Val Pro Asn Leu Gln Val Pro Pro His Gly Val Phe Tyr
225 230 235 240
Ser Gly Pro Asp Ser Ser Leu Ser Ser Pro Ser Arg Ser Pro Met Arg
245 250 255
Ala Phe Gly Thr Asp Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys
260 265 270
Pro Tyr Pro Glu Ile Asn Phe Val Gly Ser Gly His Cys Ser Ser Pro
275 280 285
Gly Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser
290 295 300
Gly Pro Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro
305 310 315 320
Ile Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln
325 330 335
Ser Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Gly Thr Pro Thr
340 345 350
Glu Ser Gln Thr Gly Arg Val Asp Asp Gly Lys Gln Gln Ser His Arg
355 360 365
Leu Pro Leu Pro Pro Leu Thr Val Thr Asn Thr Ser Pro Phe Ser His
370 375 380
Ser Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Ala Arg
385 390 395 400
Ala Asp Ser Pro Met Ser Ser Gly Ser Arg Trp Lys Lys Gly Lys Leu
405 410 415
Leu Gly Arg Gly Thr Phe Gly His Val Tyr Ile Gly Phe Asn Ser Gln
420 425 430
Ser Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp
435 440 445
Ala Lys Ser Leu Glu Ser Ala Lys Gln Leu Thr Gln Glu Val His Leu
450 455 460
Leu Ser Arg Leu Arg His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu
465 470 475 480
Thr Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ser Gly Gly
485 490 495
Ser Ile His Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala
500 505 510
Ile Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His
515 520 525
Ala Lys Asn Thr Leu His Arg Asp Ile Lys Gly Ala Asn Ile Leu Val
530 535 540
Asp Pro Asn Gly Arg Val Lys Val Ala Asp Phe Gly Met Ala Lys His
545 550 555 560
Ile Thr Gly Gln Tyr Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp
565 570 575
Met Ala Pro Glu Val Ile Lys Asn Ser Lys Glu Cys Ser Leu Gly Val
580 585 590
Asp Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys
595 600 605
Pro Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly
610 615 620
Asn Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly
625 630 635 640
Lys Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Arg Asp Arg Pro
645 650 655
Ser Ala Ser Glu Leu Leu Glu His Pro Phe Val Lys Gly Ala Ala Pro
660 665 670
Leu Glu Arg Pro Ile Met Val Pro Glu Ala Ser Asp Pro Ile Thr Gly
675 680 685
Ile Thr His Gly Thr Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu
690 695 700
Ser Ala Leu Asp Ser Asp Lys Leu Leu Val His Ser Ser Arg Val Leu
705 710 715 720
Lys Asn Asn Pro His Glu Ser Glu Ile His Ile Gln Arg Asn Ile Ser
725 730 735
Cys Pro Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Ser Arg Ser Pro
740 745 750
Gln Gln Arg Ser Gly Arg Leu Ser Pro Ser Pro Ile Ser Ser Pro Arg
755 760 765
Thr Ala Ser Gly Ala Ser Thr Pro Leu Thr Gly Gly Ser Gly Ala Ile
770 775 780
Pro Phe Ser Asn His Leu Lys Gln Ser Val Tyr Phe Gln Glu Cys Leu
785 790 795 800
Gly Ser Met Pro Lys Ser Pro Asn Gly Ala Tyr Ile Asn Gly Ser Ser
805 810 815
His His Asp Ser Asn Ile Asp Ile Phe Gln Arg Met Gln Ala Gly Ser
820 825 830
His Ile Lys Ser Glu Leu Val Ser Gly Asp Asn Asp Ala Leu Gly Lys
835 840 845
Gln Phe Val Arg Ser Pro His Ala Glu Pro Tyr Asp Phe Gln Ser Val
850 855 860
Leu Ala Asp Arg Val Gly Arg Gln Leu Leu Gly Asp His Val Lys Ile
865 870 875 880
Asn Pro Ser Phe Asp Pro Ser Pro Ser Ser Ser Met Leu Asn Arg Thr
885 890 895
Asn Gly Leu
<210> 20
<211> 894
<212> PRT
<213> 百脉根(Lotus japonicus)
<400> 20
Met Pro Ser Trp Trp Gly Lys Pro Ser Ser Lys Glu Thr Lys Lys Lys
1 5 10 15
Ala Ser Lys Glu Ser Phe Ile Asp Thr Leu Gln Arg Lys Phe Lys Ser
20 25 30
Pro Ser Glu Gly Lys Leu Ser Ser Arg Ser Gly Gly Ser Gly Arg Arg
35 40 45
Cys Asn Asp Thr Ile Ser Glu Lys Gly Ala Gln Ser Pro Pro Glu Ser
50 55 60
Arg Ser Pro Ser Pro Ser Lys Val Ala Arg Cys Gln Ser Phe Ala Glu
65 70 75 80
Arg Pro Arg Ala Gln Pro Leu Pro Leu Pro Gly Leu His Pro Ser Asn
85 90 95
Ile Ser Arg Ala Asp Ser Glu Ile Ser Ile Ser Ser Lys Ile Arg Gln
100 105 110
Glu Lys Ser Ser Lys Gln Ser Leu Phe Pro Pro Leu Pro Arg Pro Ala
115 120 125
Cys Met Arg Gly Arg Leu Asn Pro Gly Asp Leu Asp Ser Asp Leu Ile
130 135 140
Thr Ala Ser Val Ser Ser Glu Ser Ser Ala Asp Ser Asp Glu Pro Leu
145 150 155 160
Asp Ser Arg Asn Arg Ser Pro Leu Ala Thr Asp Ser Glu Thr Gly Thr
165 170 175
Arg Thr Ala Ala Gly Ser Pro Ser Ser Leu Met Leu Lys Asp Gln Ser
180 185 190
Ala Ala Val Ala Gln Val Asn Ser Arg Glu Ala Lys Lys Pro Ala Asn
195 200 205
Ile Leu Gly Asn Met Ser Ser Thr Ser Pro Lys Arg Arg Pro Leu Ser
210 215 220
Asn His Val Pro Asn Leu Gln Ile Pro Pro His Gly Ala Phe Cys Ser
225 230 235 240
Ala Pro Asp Ser Ser Arg Ser Ser Pro Ser Arg Ser Pro Leu Arg Ala
245 250 255
Phe Gly Thr Glu Gln Val Leu Asn Ser Ala Phe Trp Ala Gly Lys Pro
260 265 270
Tyr Thr Glu Val Asn Phe Val Gly Ser Gly Gln Cys Ser Ser Pro Gly
275 280 285
Ser Gly His Asn Ser Gly His Asn Ser Met Gly Gly Asp Met Ser Gly
290 295 300
Gln Leu Phe Trp Gln Pro Ser Arg Gly Ser Pro Glu Tyr Ser Pro Val
305 310 315 320
Pro Ser Pro Arg Met Thr Ser Pro Gly Pro Ser Ser Arg Ile Gln Ser
325 330 335
Gly Ala Val Thr Pro Ile His Pro Arg Ala Gly Val Thr Ser Ala Glu
340 345 350
Ser Gln Ile Gly Arg Val Asp Asp Gly Lys Lys Gln Ser His Arg Leu
355 360 365
Pro Leu Pro Pro Leu Ala Val Thr Asn Ser Met Pro Phe Ser His Ser
370 375 380
Asn Ser Ala Ala Thr Ser Pro Ser Met Pro Arg Ser Pro Gly Arg Ala
385 390 395 400
Asp Asn Pro Met Ser Pro Gly Ser Arg Trp Lys Lys Gly Lys Leu Leu
405 410 415
Gly Arg Gly Thr Phe Gly His Val Tyr Val Gly Phe Asn Lys Glu Ser
420 425 430
Gly Glu Met Cys Ala Met Lys Glu Val Thr Leu Phe Ser Asp Asp Ala
435 440 445
Lys Ser Lys Glu Ser Ala Lys Gln Leu Met Gln Glu Ile Ser Leu Leu
450 455 460
Ser Arg Leu Gln His Pro Asn Ile Val Gln Tyr Tyr Gly Ser Glu Thr
465 470 475 480
Val Asp Asp Lys Leu Tyr Ile Tyr Leu Glu Tyr Val Ala Gly Gly Ser
485 490 495
Ile Tyr Lys Leu Leu Gln Glu Tyr Gly Gln Phe Gly Glu Leu Ala Ile
500 505 510
Arg Ser Tyr Thr Gln Gln Ile Leu Ser Gly Leu Ala Tyr Leu His Ala
515 520 525
Lys Asn Thr Ile His Arg Asp Ile Lys Gly Ala Asn Leu Leu Val Asp
530 535 540
Thr Asn Gly Arg Val Lys Leu Ala Asp Phe Gly Met Ala Lys His Ile
545 550 555 560
Thr Gly Gln Ser Cys Pro Leu Ser Phe Lys Gly Ser Pro Tyr Trp Met
565 570 575
Ala Pro Glu Val Ile Lys Asn Ser Ser Gly Cys Asn Leu Ala Val Asp
580 585 590
Ile Trp Ser Leu Gly Cys Thr Val Leu Glu Met Ala Thr Thr Lys Pro
595 600 605
Pro Trp Ser Gln Tyr Glu Gly Val Ala Ala Met Phe Lys Ile Gly Asn
610 615 620
Ser Lys Glu Leu Pro Thr Ile Pro Asp His Leu Ser Asn Glu Gly Lys
625 630 635 640
Asp Phe Val Arg Lys Cys Leu Gln Arg Asn Pro Gln Gln Arg Pro Ser
645 650 655
Ala Ser Asp Leu Leu Asn His Pro Phe Val Lys Cys Ala Ala Pro Leu
660 665 670
Glu Arg Pro Ile Leu Leu Pro Glu Ala Ser Asp Pro Val Ser Glu Ile
675 680 685
Ala His Gly Ser Lys Ala Leu Gly Ile Gly Gln Gly Arg Asn Leu Ser
690 695 700
Met Leu Asp Ser Asp Arg Leu Ser Leu His Ser Arg Phe Leu Lys Asn
705 710 715 720
Asn Pro His Ala Ser Glu Leu His Ile Pro Arg Asn Ile Ser Cys Pro
725 730 735
Val Ser Pro Ile Gly Ser Pro Leu Leu Arg Pro Arg Ser Pro Gln His
740 745 750
Met Ser Gly Arg Met Ser Pro Ser Pro Ile Ser Ser Pro Arg Thr Ala
755 760 765
Ser Gly Ala Ser Thr Pro Leu Ser Gly Gly Ser Gly Ala Ile Pro Phe
770 775 780
Ser Asn Asn Leu Val Tyr Phe Gln Glu Gly Leu Gly Ser Leu Pro Lys
785 790 795 800
Ser Pro Asn Gly Ile Tyr Ile Ser Gly Pro Ala His Pro Asp Ser Asn
805 810 815
Val Asp Ile Phe Arg Gly Met Gln Lys Thr Ser His Ile Ser Ser Glu
820 825 830
Leu Val Pro Ser Glu Asn Asp Ala Leu Gly Lys Gln Phe Thr Arg Leu
835 840 845
Pro Gln Asp Lys Ser Tyr Asp Val Gln Ser Val Leu Ala Asp Arg Val
850 855 860
Cys Arg Gln Leu Leu Gly Asp Asn Val Lys Ile Asn Pro Ser Ile Asp
865 870 875 880
Leu Arg Pro Asn Thr Asn Leu Leu Ser Arg Ala Asn Gly Leu
885 890
<210> 21
<211> 2649
<212> DNA
<213> 鹰嘴豆(Cicer arietinum)
<400> 21
atgccttcat ggtgggggaa attatcatca aaagaaacca agaagaaagc aagtaaggaa 60
agtatttttg acacattgca ccgaaaattt agatttccat ctgagggtaa actaagcact 120
atatcaggag aatcacgtag acgatgcagt gacacaattt cagagaaagg ggatcgttct 180
ccatctgaat caagatctcc ttcaccttcc aaagtagcaa ggtgtcaaag tttttccgaa 240
aggcctcatg ctcagccact tccacttcct ggtctgcacc cttcaagtgt aggtcgagtg 300
gattctgaaa ttagtatatc agtgaaatca agacttgaaa agagctccaa gccgtcattg 360
tttcttcctc ttccaaaacc tgcatgcata cgttgtggac caagtcctgc agatttggat 420
ggagatttgg tcactaattc agtcttcagt gattgctctg ccgatagtga tgagccagca 480
gactcacgca atcgtagtcc tctggcaact gactctgaaa ccgggactag aactgctgct 540
ggaagtcctt ccagcttgat gctcaaggat caaacttctg ctgtttccca actgaattca 600
agagaagtca aaaaaccgac aaacattcta agtaatcaca cgtcttctac ttcaccaaaa 660
cggaggcctt tacgcaacca cgtaccaaat cttcaggttc ctccccacgg tgtcttttat 720
agtggtcctg acagttcctt gtcaagtcca tcaagaagtc cattgagggc atttggtaca 780
gatcaggtat tgaactctgc tttttgggcc ggaaagccat atccagaggt caatttcctt 840
ggatctgggc actgctctag tccaggttct ggtcacaact ctgggcataa ttcaatggga 900
ggggacatgt cgggaccatt attttggcaa ccaagcaggg gtagccctga gtattctccg 960
gtaccgagtc ctagaatgac tagccctggt ccaagctcta gaattcagag tggagctgtc 1020
acacctattc atcctcgagc agcaggaacg ccaactgaat cacaatcagg atgggttgat 1080
gatggaaaac aacagagtca tcgtttgccc ctccctcctt taactgttac caattcctcg 1140
ctgttttctc actctaattc tgcagcaaca tctccatcta tgccaagaag tccagctaga 1200
gcagatagtc caatgagctc tggttcacgt tggaaaaaag gaaagctgct tggcagaggc 1260
acatttggac acgtctatat tggcttcaat agtgaaagtg gtgaaatgtg tgcaatgaag 1320
gaggttacta tgttttcaga tgatgccaag tctatggaaa gtgctaagca attaatgcag 1380
gaaattcatt tattgagccg tttaaggcat ccaaatattg tgcagtatta tggctctgaa 1440
acagttgacg acaagcttta catatacctc gagtatgtat ctggaggctc catacataaa 1500
cttcttcaag aatatgggca attcggtgaa ctagctattc gtagttatac tcaacaaatt 1560
ttgtcagggc ttgcttattt acatgctaaa aatactcttc acagggacat caaaggagca 1620
aacatactgg tagatccaaa tggtcgggtc aaggttgcag actttggcat ggcaaaacat 1680
ataacagggc aatactgtcc cttgtcattc aaaggaagtc catattggat ggctcctgag 1740
gttataaaaa actctaaggg atgcagcctt gctgtggata tatggagtct tggatgcaca 1800
gttttggaaa tggctacaac taagcctcct tggtctcaat atgaaggggt tgctgctatg 1860
ttcaaaattg gtaacagcaa ggagcttcca gcaatcccag atcatctctc aaatgaagga 1920
aaagattttg ttagaaaatg tctacaacgt aatccacgtg atcgcccttc agcaattgaa 1980
ttattggacc acccttttgt aaaatctgct ggacctttgg aaagacctat tatggttcct 2040
gaagcttcag acactatgtc tggaattaca cacggaacaa aagctctggg cattggacaa 2100
ggaagaaatc cgtctgcctt ggattcagat aaactctccg ttcattcttc tagggttttg 2160
aaaatctctc ccattggaag cccacttttg aggtcaagat caccacaaca gagaagtgga 2220
agattgtctc cttctcctat atctagccct cggactgctt cgggtgcatc cacgcctctc 2280
actggcggca gtggtgccat tccattcagt aataacctaa aacagtcagt ttactttcaa 2340
gaatgtcttg gaagcatgcc gaaatcctca aacagtgtct acattaacgg ctccactcat 2400
cacgactcaa atattgacat ttttcgagga atgcaaattg ggccacacat taaatcagat 2460
cttgtctcca gtgaaaatga tgttctcggt aagcagtttg tcaggtctcc tcatgtagag 2520
ccgtatgact ttcagtcagt cttggctgat cgtgttggcc ggcagttgct cggggaccat 2580
gttaagatta acccttcgtt cgatccatgt cccagtccgt cattgctcaa ccgaacaaac 2640
ggtttatga 2649
<210> 22
<211> 1999
<212> DNA
<213> 豌豆(Pisum sativum)
<400> 22
atgcctacat ggtggggaaa atcatcatca aaagaaacca ggaagaaagc aggtaaggaa 60
agtattattg acacattgca ccgaaaattt aaatttccat ctgagggtaa attaagcact 120
agatcaggag gatctcgtag acgaagcaat gacacaatct cagagaaagg ggatcgttct 180
ccatccgaat caagatcgcc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggccacatg ctcaaccact tccgctccct ggtctgcacc cttcaagtgt aggtcgagtg 300
gattctgaaa ttagtatatc agcaaaatca agactggaaa agtcctccaa atccttattg 360
tttcttccac ttccgaaacc tgcgtgcata cgctgcgggt caaatcctgc agatttagat 420
ggagatatgg tcaacacttc agtcttcagt gattgctctg ctgatagtga cgagccagca 480
gactcacgca atcgtagtcc tctggcaact gactccgaaa ctgggactag aactgctgct 540
ggcagtcctt ccagcttgat gctcaaggat caaccgtctg ctgttgccca actgaattcg 600
agagaagtga aaacaccgac aaacatttta agtaatcaca caccttctac ttcacctaag 660
cgaaggcctt tacgacacca tgttccaaat cttcaggttc ctcctcatgg tgtcttttat 720
agtggtcccg acagttcctt gtcaagtcca tcaagaagtc cgctgagggc atttggtaca 780
gatcaggtgt tgaattctgc attttgggct ggaaagccat atccagagat caatttcgtc 840
ggatctggcc actgctctag tccaggttcc ggtcacaatt cggggcataa ttcaatggga 900
ggggacatgt cgggaccatt attttggcaa ccaagtaggg gtagccctga gtattctccg 960
gtacctagtc ctagaatgac tagccctggt cctagctcaa gaattcagag tggagcagtc 1020
acacctattc atcctagagc agcgggaaca ccgactgaat cacaaacagg gtgggttgat 1080
gagggaaaac aacagagtca ccgtttgcct ctccctcctt taacagttac caatacctcg 1140
ccgttctctc attctaattc tgcagcaaca tctccatcta tgccaagaag tccagctaga 1200
gcagatagtc caatgagctc tggttcacgt tggaaaaaag gaaagctgct tggcagaggc 1260
acgtttggac atgtctatat tggcttcaat agtgaaagtg gtgaaatgtg tgcaatgaag 1320
gaggttacta tgttttcaga tgatgccaag tctatggaaa gtgctaagca attaatgcag 1380
gaaattcatt tgttgagccg tttaagacat ccaaatattg tgcagtatta tggttctgaa 1440
aaagtagatg acaagcttta catatacctt gagtatgtat ctggaggctc catacataaa 1500
cttcttcaag aatatgggca atttggtgaa ctagctattc gtagttatac tcaacaaatt 1560
ttgtcagggc ttgcttattt acatgctaaa aatactcttc atagggacat caaaggagca 1620
aacatactgg tagatccaaa tggtcgggtc aaggttgcag actttggcat ggcaaaacat 1680
ataacagggc aatactgtcc tttgtcgttc aaaggaagtc cttattggat ggctcccgag 1740
gttataaaaa attccaagga atgcagcctt ggtgtggata tatggagtct tggatgcaca 1800
gttttggaaa tggctacaac taagcctcct tggtctcaat atgaaggggt tgctgccatg 1860
ttcaaaattg gtaacagcaa ggagctccca acaatcccgg aacatctttc aaatgaagga 1920
aaagattttg ttaggaaatg tctacaacgt aatccacgtg atcgcccttc agcaagtgaa 1980
ttattggacc acccttttg 1999
<210> 23
<211> 4020
<212> DNA
<213> 大豆(Glycine max)
<400> 23
gtgaacactg attgagacgg agaccccttc tttcaccctc tctttctctc tctccttctc 60
tacgcgatct ctttcactta cttttaattg cgaatatttt caccacaaat cacttcaaaa 120
tccttctctg tttgttgcat tttgagtcac ggagtccttt ggggttctct ctctctcgcg 180
ccgagctccg aacgcttcag ggaatttcct cactcagatt tgagctgaaa attgcgcagt 240
gagttcactt caaattcaga gctgattaat tgaagtgctt gtttttaaca atggattgat 300
agtgcatctt gggatcctgg aaattatggt ggaattagag agagggaatg tgaaagtttg 360
agtttttgtt ctttttttct ctctgggtat gtgggggaat ctgggtatgg agacacacaa 420
aaaaagttat catcatggat aggttttttg caataatagt tcaataactg actcctgtct 480
ctaattctga agttattgct tttgcaattt gtgattctcc cacttttaaa ggttaatggc 540
tgcgtcatgg cttttgtaat tctgtctact gatgtgaaat gatagatttt tggaactatt 600
actgggatcc ttttaagaaa cttgcttgcc acatcttaat ttataaaata caaatatgct 660
tacatggtgg gggaaatcat catcaaaaaa aaccaagaag aaagcaaata aggaaagttt 720
ttttgacaca ctgcaccgaa aactcagaat ttcatctgaa ggtaaagtaa acattagatc 780
tggaggatct cgtaggcatt gcaatgacac aatttcagag aagggggatc attctccatc 840
tggatcaaga tcgccttcac cttccaaagt ggcaaggtgt caaagtttta ttgataggcc 900
tcatgctcag ccacttccac ttcctggtct gcacccttca agtgtaggcc gagtagattc 960
cgaaattagc atatcatcaa aatcaagatt ggaaaaagtc tccaagccat cattgtttct 1020
tccacttcca acacctggat gcatacgttg taggccgaac cctgcagatt tggatggaga 1080
tatggtcact ccttcagtct ttagtgattg ctctgctgac agtgatgagc cggcagactc 1140
acacaatcgt agtcctctag caactgactg tgagactggg actagaactg ctgctggcag 1200
tccttccagc tcgatgctca aggatcaacc acctactgtt tccctactga attcaacagg 1260
agtaaagaaa ccaggaaata ttctaagtaa tcatatgtct tctacttcac caaaacatag 1320
gcctttacgc aaccatgttc caaatcttca ggttcctcct catggtgcct tctatagtac 1380
tcctgatagt tccttgtcaa gtccatcaag aagtccattg agagcatttg gcacagatca 1440
ggtgttgaat tctgcttttt tggctggaaa gccatatcca gaggtcaatt ttgttgggtc 1500
tgggcattgc tctagtccag gttcaggtca caattctggg tataattcaa tgggagggga 1560
catgtcagga ccgttacttt ggcaaccaag caggggtagc cctgagtatt ctccagtacc 1620
tagtcccaga atgactagcc ctggtccaag ctctagaatt cagagtggag cagtcacacc 1680
tattcatccc aaagccgggg gaacacccac agaatcacag actcatcgtt tgcctcttcc 1740
tcctttgtca gtttctaatt cctcaccgtt ctctcattca aattctgcag caacatctcc 1800
atctatgcca agaagtccag ctagagcaga taatccaagc tctggctcac gttggaagaa 1860
agggaagctg cttggcagcg gctcatttgg acatgtctat cttggtttca atagtgaaag 1920
tggcgaaatg tgtgcagtga aggaggttac cctgttttca gatgatccca agtctatgga 1980
aagtgctaag caatttatgc aggaaattca tttattaagc cgtttacagc atccaaatat 2040
cgtccagtat tatggttctg aaacagttga tgacaagctt tacatatacc ttgaatatgt 2100
atctggaggc tccatacata aacttcttca agaatatggg caatttggtg aactagttat 2160
tcgtagttat actcaacaga ttttgtcagg gcttgcttat ttgcatgcta aaaatactct 2220
ccatagggat atcaaaggag caaatatact ggtagatcca actggtcggg tcaagttggc 2280
agactttggc atggcaaaac atataacagg gcaatcgtgt ctattgtcat tcaagggaac 2340
cccttactgg atggctcctg aggttataaa gaactctaat ggatgcaacc ttgcagtgga 2400
tatatggagt cttggatgca cggttttgga aatggctact accaaacctc cttggtttca 2460
gtatgaagcg gttgctgcca tgttcaagat tggtaatagc aaggaactcc caacaatccc 2520
tgatcatctc tcaaatgaag gaaaagattt tgttaggaaa tgtcttcagc gtaacccgta 2580
tgatcgccct tcagcctgtg aattgttgga ccaccctttt gtaaaaaatg ctgcaccttt 2640
ggaaagacct attctggctc ctgaagtgtt ggaccctgtt tctgggatca tacagggagc 2700
aaaagctctg gccgctggac aaggaaagaa tctttctagc ttggattcag atagactttc 2760
tattcattct tctagatttt tgaaaactaa tcctcgtgaa agtgaaatcc atattccaag 2820
gaatatatct tgccctgttt ctcccattgg aagcccactt ttgaggtcaa gatcaccaca 2880
gcacaggaat gggaaaatgt ctcctcctat atctagccct cggactgctt ctggggcgtc 2940
cacccctctt gctggtggca gtggtgccat tccatttggt aatcactcta aacagccaat 3000
ttactttcaa gagggttttg gaagcattcc caagtcctca aatggtgtct acattaatgg 3060
ccattctcat cacgactcga gtgttgacat ttttcgagga atgcaaatag gatctcacat 3120
tcaaccagaa ttggtttcca gtgaaaatga tgttttggtt aatcagtttg caaggcatcc 3180
tcatgcagag ccatatgatt ttcagtcagt cttggcagat cgtgttggcc ggcagctgct 3240
gagggaacat gttaagatta acccatccat tgatctcagt cccaactcgt ctttgcttag 3300
ccggccaaac ggtttatgac atcaagattt ttccttaagt ttctcatagt acctttggtt 3360
atgcagatct gttcattgtt ctatttgcaa gaatataact atacaataag gagccaaagc 3420
acaagagaat cgatgtaaat aaatttgcat ctgagaatag gctacatggg gcacaaaagt 3480
gcattttgca tggcttctgc tcgggcttat ggagttcatt aatgtctcag cattaaactt 3540
tctgacaatt tgttcatatc aagcaatcaa ctaacaaatg ctcacttgtt cgaaaagagg 3600
aacttgttgt atgtaacata tttaaagcat caacgcatga ggggtttctc tcctgcactt 3660
aagatactag aaatgacagt tcgtgcaatg gcaaatgcat cttctggaat cagaacacca 3720
tatagatgcc agagaaaggg ctaaattgat acaaaactta acttgcatat ttttcttttt 3780
tgggaggtta gacattgctg tacagtagat gggaaactgt tttgtgattc tgcatttacg 3840
aatggggaag tcaaggtttt gtagagagtc catgcatggt ttttgggctc tgatgtattg 3900
ttgtcagtgt atactctact actgggtctg ttctgtacat tattattcaa cagtaaaatg 3960
gaagaaatct gatcttatga tcctcattag attatacatg gctttggaac aaacatgctt 4020
<210> 24
<211> 3909
<212> DNA
<213> 大豆(Glycine max)
<400> 24
acactgattg agacggagac cccttctttc actctttctc tctccttctc tacgcgatct 60
ctttcactta cttttaattg cgaatatttt caccacaaat cacttcaaaa tccttctctg 120
cttgtcgcat tttgagtctc tgtgggtctt ttggggctct ctctctcgcc gagctccgca 180
ctcttcaggg aatttcctca ctcagattcc agctgaaaat tgcgcagtga gttcacttca 240
aattcagaga tgattaattg aagttttttg caataatagt tcaataactg attcctgtct 300
ctaattctga agttattact tttaaaggtt taatggctgt gtcatgactt ttgtaacttt 360
gcctactgaa gtgaaatgat agattttttg aactcttact gggatccttc taagaaactt 420
gcgtgccaca tctcaattta taaaatacaa atatgcttac atggtgggga aaatcatcat 480
caaaagaaac caagaagaaa gcaaataagg aaagtttctt tgacacactg caccgaaaac 540
ttagaatttc atccaaaggt aaagtaagca ttagatctgg aggatcacgt agacattgca 600
atgacacaat ttcagagaag ggagatcatt ctccatgtgg atcaagatcg ccttcacctt 660
ccaaagtggc aaggtgtcaa agttttattg ataggcctca tgctcagcca cttccacttc 720
ctggtctgca tccttcaagt gtaggccgag tagattctga aattagcata tcatcaaaat 780
caagattgga aaaagtctcc aagccattat cgtttcttac gcttccaaca cctggatgca 840
tacgttgtag gccaaaccct gcagatttgg atggagatat ggtcactgct tcagtcttta 900
gtgattgctc tgctgacagt gatgagccgg cagactcaca caatcgtagt cctctagcaa 960
ttgactgtga gactgggact agaactgctg ctggcagtcc ttccagctta atgctcaagg 1020
atcaaccacc tgctgtttcc caactgaatt caacgggagt aaagaaacca ggaaatattc 1080
taagtaatca tatgtcttct acttcaccaa aacgtaggcc tttacgcaac catgttccaa 1140
atcttcaggt tcctcctcat ggtgccttct atagtgctcc tgatagttcc ttgtcaagtc 1200
catcaagaag tccattgagg gcatttggca cagatcaggt gttgaattct gcttttttgg 1260
ctggaaagcc atatccagag atcaattttg ttgggtctgg gcattgctct agtccaggtt 1320
caggtcacaa ttctgggcac aattcaatgg gaggggacat gtcaggaccg ttactttggc 1380
aaccaagcag gggtagccct gagtattctc cagtacctag tcccagaatg actagccctg 1440
gtccaagctc tagaattcag agtggagcag tcacacctat tcatcccaaa gccgggggaa 1500
cacccacaga atcacagact catcgattgc ctcttcctcc tttgtcagtt tctaattcct 1560
cactgttctc tcattcaaat tctgcagcaa catctccgtc tatgccaaga agtccagcta 1620
gagcagataa tccaaactct ggctcacgtt ggaagaaagg gaagctgctt ggcagcggct 1680
catttggaca tgtctatctt ggcttcaata gtgaaagagg cgaaatgtgt gcagtgaagg 1740
aggttaccct gttttcagat gatcccaagt ctatggaaag tgcgaagcaa tttatgcagg 1800
aaattcattt attaagccgc ttacagcatc caaatattgt ccagtattat ggttctgaaa 1860
cagtcgataa caagctttac atataccttg agtatgtatc tggaggctcc atacataaac 1920
ttcttcgaga atatgggcaa tttggtgaac tagttattcg tagttatact caacaaattt 1980
tgtcagggct tgcttatttg catgctaaaa atactctcca tagggatatc aaaggagcaa 2040
atatactggt agatccaact ggtcgggtca agttggcaga ctttggcatg gcaaaacata 2100
taacagggca atcgtgccca ttgtcattca agggaacacc ttactggatg gctcctgagg 2160
ttataaaaaa ttctaatgga tgcaaccttg cggtggatat atggagtctt ggatgcacag 2220
ttttggaaat ggctacaacc aaacctcctt ggtttcagta tgaaggggtt gctgccatgt 2280
tcaagattgg taatagcaag gaactcccaa ccatccctga tcatctctca aatgaaggaa 2340
aagattttgt taggaaatgt cttcagcgta acccacatga tcgtccttca gccagtgaat 2400
tgttggacca cccctttgta aaaaatgctg cacctttgga aagacctatt ccggctcctg 2460
aagctttgga ccctgtttct gggattacac agggagcaaa agctctggct attggacaag 2520
gaaggaatct ttctagcttg gattcagata gactttctgt tcattcttct agatttttga 2580
aaactaatcc tcatgaaagt gaaatccata ttccaaggaa tatatcttgc cctgtttctc 2640
ccattggaag cccacttttg aggtcaagat caccacagca caggaatggg aaaatgtctc 2700
cttctcctat atctagccct cggactgctt ctggggcatc tacacctctt gctggtggca 2760
gtggtgccat tccatttggt aatcactcta aacagtccat ttactttcaa gagggttttg 2820
gaagcattcc caagtcctca aatggtgtct acatgaatgg ccattctcat cacgactcga 2880
atgttgacat ttttcgagga atgcaaatgg ggtctcacat ttcaccagaa ctggtttcca 2940
gtgaaaatga tgttttggtt aagcagtttg caaggcatcc tcatgcagag ccatatgatt 3000
ttcagtcggt cttggcagat cgtgttggcc ggcagctgct gggggaacat gttaagatta 3060
acccatccat tgatctcagt cccaactcgt ctttgcttag ccggccaaac ggtttatgac 3120
atcaagattt ttcctaaagt ttctcataat acctttggtt agcagatctg ttcattgttc 3180
tatttgcaag aatataacta tacaataagg agccaaagca caagagaatc aatgtaaata 3240
aatttgtatc caagaatagg ctacatgggg cacaaaagtg cattttgcat ggcttcggct 3300
cgggctgatg gagttcattc gtgccacagc attaaacatt ctgacaattt gtccatatca 3360
agcaatcaac tcatacaaat gctcacttgt tcgaagagga acttcttgtt gtatgtaaca 3420
tatttaaagc atcaacgcat gagaggtttc tctcctgcac ttaagatact agaaatgaca 3480
gtttatgcaa tgacaaatgc atcttctaga attggaacac catatagatg ccagagaaag 3540
ggttaaattg atacaaaact taacttgcag actttttttt gggaggttag acattgctgt 3600
acggtagatg ggaaactgta ttgtgatcat gcatttatgg atggggaagt caaggtttgt 3660
agagagtcca tgcatgatgc atgttatgtt taattgtggg agatgaagta ttattgtcat 3720
tgtatagtct actactggat ttgttctgta cattattatt caacagtaaa atggaagaaa 3780
tctaaaaatc ttatgatcct catcggatta tacagggctt tggaacaaat ctggttgggt 3840
gagaaaaatt ggatgctttg aaagcaccac tgaatatata ttactggcca tcacgatccc 3900
gttttgatt 3909
<210> 25
<211> 2772
<212> DNA
<213> 菜豆(Phaseolus vulgaris)
<400> 25
atgatagatt ttttaactat tactgagatc cttttaagaa gtttgcttgc cacatttcaa 60
tttataaaga caaatatgct tacatggtgg ggaaaatcat catcaaaaga aagcaagaag 120
aaatcaaata aggaaagctt ttttgacaca ctgcaccgga aatttaaaat ttcatccgaa 180
ggtaaactaa gaactagatc tggaggatcc cgtagacatc gcaatgacac agtttcagag 240
aagggggatc cttctccaac tgaatcaaga tcaccttcac cttccaaagt ggctaggtgt 300
caaagtttta ttgaaagacc tcatgctcag ccacttcctc ttcctggtct gcacccttca 360
agtgttggcc gagtagattc tgaaattagc atatcatcaa aatcaagatt ggaaaaaggc 420
tccaagccat cattgtttct tcctctccca acacctggat gcatacgttg taagccaaac 480
cctgcagatt tggatggaga tatggtcaca gcttcagtct ttagtgattg ctctgctgac 540
agtgatgagc cacactcaca caatcgaagt cctctagtgg ctgactgtga gactgggagt 600
agaactgctg ctgctggcag tccttccagc acgatgctca aggatcagcc aattgttgtt 660
tcccagctca attcagcagg agtaaaaaaa cccggaagta ttctaagtaa tcatacatct 720
tctacatccc caaaacgtag gcctttacgc aaccatgttc caaatcttca ggttcctcct 780
catggtgcct tctacagtgc tcctgatagt tccttgtcaa gtccatccag aagtccattg 840
agagcatttg gcacagatca ggttttgaat tctgcttttt ggtctggaaa gccatattca 900
gaggtcaact tggttggatc tggacagtgc tctagtcctg gttcaggtca caattctgga 960
cataattcaa tgggagggga catgtcagga cctttatttt ggcaaccaag caggggtagt 1020
cctgagtatt ctccagtacc tagtcccaga atggctagtc ctggtcctag ctcgagaatt 1080
cagagtggag cagtcacacc tattcatccc aaagctgggg gaacacccat tgaatcacag 1140
acaggaaggg ttgatgatgg aaaacagcag actcatcgat tgccgcttcc tcctttgtca 1200
gtctccaatt cctcaccatt ctctcattca aattcggcag caacatctcc gtctatgcca 1260
agaagtccag ctagagcaga tactccaggc tctggctcac gttggaagaa aggaaagctg 1320
cttggtagtg gctcatttgg acatgtctat cttggcttca atagtgaaag tggcgaaatg 1380
tgtgcagtga aggaggttac cctgttttca gatgatccca agtcaatgga aagtgctaag 1440
caatttatgc aggaaattca tttattaagc cgtttacagc atccaaatat tgtccagtat 1500
tatggttctg aaacagttga tgacaagctt tacatatacc ttgaatatgt atctggaggc 1560
tccatacaca aacttcttcg agaatatggg cagtttggtg aactagttat tcgtagctat 1620
actcaacaaa ttttgtcagg gcttgcttat ttgcatgcta aaaatactct ccatcgggac 1680
atcaaaggag cgaatatact ggtagatcca actgggcggg tcaggttggc agactttggc 1740
atggcaaaac atataactgg gcaatcgtgt ccattatcat tcaaaggaac cccttactgg 1800
atggctcctg aggttataaa gaactctaat ggatgcaatc ttgcagtaga tatatggagt 1860
cttggatgca cagttttgga aatggctact accaaacctc cttggtttca gtatgaaggg 1920
gttgctgcca tgttcaagat tggtaatagc aaggaactcc cgacaatccc tgatcatctc 1980
tcaaatgaag gaaaagattt tgttaggaaa tgtcttcagc gtaacccata tgatcgccct 2040
tcagccagtg aattattgga ccaccctttt gtaaaacttg ctgcaccttt ggaaagacct 2100
attctggtta ctgaagcatt ggaccatgtt tctgggacta cacaaggggc aaaagctctg 2160
gcaattggac aagcaaagaa tctttctacc ttggattcag atagactttc tgttcattct 2220
tcaagatttt tgaaaattaa tccccatgaa agtgaaatcc atattccacg gaatatatct 2280
tgccccgtct ctcccattgg aagcccactt ttgaggtcaa gatcaccgca gcacaggaat 2340
gggagaatgt ctccttctcc tatatccagc cctcggactg cttctggggc atcaacacct 2400
ctcgctggtg gcagtggtgc cattccattt ggtaatcact ctaaacagtt aatttacttt 2460
caagagggtt ttggaagcat ccctaagtcc tcaaatggta tgtacgtcaa tggccctgct 2520
catcatgact caactgttga catttttcga ggaatgcaaa tagggtctca cattccatca 2580
gagctggttt ccagtgaaaa tgatgttctg gttaagcagt ttgcaaggca tcctcatgct 2640
gagccacacg attttcagtc agtcttggca gatcgtgttg gccggcagct gctgggggaa 2700
catgttagga ttaacccatc ccttgatctc agccccagct catctttgct tagccgccca 2760
aatggtttat aa 2772
<210> 26
<211> 2541
<212> DNA
<213> 赤豆(Vigna angularis)
<400> 26
atgcttacat ggtggggaaa atcatcatca aaagaaagca agaagaaatc aaataaggaa 60
agcttttttg acacactgca ccggaaatta aaaatttcat ctgaaggtaa actaagcact 120
agatctggag gatcccgtag aaattgcaat gacacagttt cagagaaggg ggatcgatct 180
ccgactgaat caagatcacc ttcaccttcc aaagtggcta ggtgtcaaag tttcattgaa 240
agacctcatg ctcagccact tcctcttcct ggtctgcacc cttcaagtgt aggccgagta 300
gattctgaaa ttagtatttc atcaaaatca agattggaaa aagtctccaa accatcattg 360
tttcttccac ttccgacacc tggatgcatc cgttgtaaac caaaccctgc agatttggat 420
ggagacttgg tcactgcttc agtctttagt gattgctctg ctgacagtga tgagccacac 480
tcacacaatc gtagtccttt agtggctgac tgtgagattg ggattagaac tgctgctggc 540
agtccttcca ggcctttacg caaccatgtt ccaaatcttc aggttcctcc tcatggtgcc 600
ttctacagtg ctccagatag ctccttgtca agtccatcaa gaagtccact gagagcattt 660
ggcacagatc aggtgttgaa ttctgctttt tgggccggaa agccatattc agaggtcaac 720
tttgttggat ctggacattg ctctagccca ggttcaggtc acaattctgg acataattca 780
atgggagggg acatgtcagg acctttattt tggcaaccaa gcaggggtag tccagagtat 840
tctcctgtac ctagtcccag aatgactagc cctggtccta gctctagaat tcagagtgga 900
gctgtcacac ctattcatcc caaagccggc ggaacaccca ttgaatcaca gacaggaagg 960
gttgatgatg ggaaacagca gactcatcgt ttgccacttc ctcctttgtc agtctccaat 1020
tcctcaccat tctctcattc aaattctgca gcaacatctc cgtctatgcc aagaagtcct 1080
gctagagcag ataatccagg ctctggctca cgttggaaga aaggaaagct gcttggtagt 1140
ggctcatttg gacatgtcta tcttggcttc aatagtgaaa gtggcgaaat gtgtgcagtg 1200
aaggaggtta ccctgttttc agatgatccc aagtcaatgg aaagcgctaa gcaatttatg 1260
caggaaattc atttattaag ccgtttacag catccaaata ttgtccggta ttatggttct 1320
gaaacagttg atgacaagct ttacatatac cttgaatatg tatctggtgg ctccatacac 1380
aaacttcttc gagaatatgg gcagtttgga gaactagtta ttcgtagcta tactcaacaa 1440
attttgtcag ggcttgctta tttgcatgct aaaaatactc tccatcggga catcaaagga 1500
gcaaatatac tggtagatcc aactggtcgg gtcaagttgg ccgactttgg catggcaaaa 1560
catataacag ggcaatcgtg tccattatca ttcaagggaa ccccttactg gatggctcct 1620
gaggttgctg ccatgttcaa gattggtaat agcaaggaac tcccaacaat cccagatcat 1680
ctctcaaatg aaggaaaaga ttttgttagg aaatgtcttc agcgcaaccc atatgatcgc 1740
ccttcagcaa gtgaattatt ggaccaccct tttgtaaaac ttgctgcacc tttggaaaga 1800
cctattctgg ttcctgaagc attggatcct gtttctggga ctacacaggg agcaaaatct 1860
ctggtgtttg aaagacctag gctcaacctc tcagttgtac tgccaaaacc gaagacccac 1920
cccaattctc cccttttctc ttcgagttat ctaatagttt gttgccatgg aaattgttct 1980
tgcatgaggc agcatgagtg gtgcagaagg aaaaggcagg gagatgaaat ccatattcca 2040
aagaatatat cttgccccgt atctcccatt ggaagcccac ttttaaggtc aagatcacca 2100
cagcacagga atgggaaaat gtctcctcct atatccagcc cacggactgc ttctggggca 2160
tccacacctc tcgctggtgg cagtggtgcc attccttttg gtaatcactc taaacagtta 2220
atttactttc aagagagttt tggaagcatc cctaagtcct caaatggtgt gtacgttaat 2280
ggccctgctc atcatgactc aactgttgac atttttcgag gaatgcaaat agggtctcac 2340
attccatcag agctggtttc cagtgaaaat gatgttctgg ttaagcagtt tgcaaggcct 2400
cctcatgcag agccatatga ttttcagtca gtcttggcag atcgtgttgg ccggcagctg 2460
ctgcgtgaac atgttaagat taacccatcc cttgatctca gtcccaactc atctttgcta 2520
agccgcccaa atggtttatg a 2541
<210> 27
<211> 3795
<212> DNA
<213> 落花生(Arachis hypogaea)
<400> 27
aaattccgaa tttttcacaa caaatcacta caagatcatt ctccattcct tgcattttgg 60
ggttttctgg gtctttttct tgttctcgct gtgctctgaa cggttcagga aattttctca 120
ctcagatttg agctgaatat tcactgtggg gggcttcagt tcagattcct ggacttgttt 180
aactgaagtt taatgcaata atttccaata actaatttct gtctctcgcc ctaaagttac 240
tgctttcgga acttgtcagc actctgtttg tgttaaggct tatggctgga tcatgatatt 300
tagaactatg gtcactgatg tgacatgata gatttttttg aactattatt tttgggatcc 360
ttttgagaaa cttgcttgac atctcaacat aacaaatatg cctatacggt gggcaaatcc 420
gttctcaaat tcaaaagaaa ccaagaagaa agcaagtaag gaaagtttta ttggcacact 480
gcatcggaaa tttaaaacac catctgaagg tagattaggc agtagatctg gaggtcctgg 540
tagacgctgc aatgacacaa tttcagagat gggggatcgt tctccagctg aatcaagatc 600
tccttcgcct tccaaagtgg caagatgtca aagtttcgct gaaaggcctc atgctcagcc 660
attaccactt cctggtcttc acccttcaag tataggccga gtagattctg aaattagcat 720
atcatcaaaa tcaagactgg aaaagagctc caagccatca ttccttccac ttccaagacc 780
tgcatgtatg cgaggaagga caaatccttc agatttggat ggagatatgg ttgcttcaat 840
ctccagtgat ggttctgctg atagtgatga ccaaacagag tcacgcaacc gaagtccttt 900
ggcaactgac accgatactg ggactagaac tgctgccgag agtccttcca gcatgatgct 960
caaggatcaa ccatctgttg tctcccaact aaattcaaga gaagccaaaa aaccagtgaa 1020
cattctaagt aatcatatgt cttctacttc accaaaacag aggcctttaa ggaaccatgt 1080
tccgaatctg caagttcccc ctcatggtgc cttctatagt gctccagaca gttccttgtc 1140
aagcccatcg agaagcccat tgagagcgtt tggcacagat caggtgttga actctgcctt 1200
ctcggctgga aagccatacc ctgagttcaa tttagccggt tctggccact gctctagtcc 1260
aggttccggt cacaattctg ggcacaattc aatgggtggg gacatgtcag gacagttatt 1320
ttggcaacca agcaggggta gccctgagta ttctccagtt cctagtccca gaatgactag 1380
tcctggtcct agctctagaa ttcagagtgg agccgtcaca cctattcatc ctagagctgg 1440
gggaacaccc actgaatcac agacaagctg ggctgatgat ggaaaacaac agagtcatcg 1500
tttgccactt cctcctttga caattaccaa ttcctcgcct ttctctcatt ccaattctgc 1560
agcaacttct ccatccatgc caagaagtcc agctagagcg gaaaatccag tgagccctgg 1620
ctcacgttgg aaaaaaggaa agctgattgg cagaggcaca tttggacatg tctacgtcgg 1680
cttcaataat gatagtggtg aaatgtgtgc aatgaaggag gttaccctgt tttcagatga 1740
tgccaagtct ttagagagcg ctaaacaatt aatgcaggaa atttctttat taagtcggct 1800
tcggcatcca aatattgtgc agtactatgg ttctgaaacg gtagatgaca agctttacat 1860
atacctggag tatgtatccg gaggctccat atataaactt cttcaagaat atggacagtt 1920
tggtgaacta gctattcgta attatactca acaaattttg tcagggcttg cttatctgca 1980
tgctaagaat actgtccaca gggacatcaa gggtgcaaat atacttgtag atccaaacgg 2040
ccgggtcaag ttggcagatt ttggcatggc aaaacatata aaagggcaat catgtccatt 2100
gtcaatcaag ggaagccctt attggatggc tcctgaggtt aagaacacta gtggatgcaa 2160
ccttgctgtg gatatatgga gtcttggatg cacggttttg gaaatggcta caaccaagcc 2220
cccttggagt caatacgaag gggttgctgc catgttcaag ataggtaata gcaaggaact 2280
cccaaaaatc ccagatcacc tctcaaatga aggaaaagat tttgtaagga gatgtctaca 2340
acgtgatcca catgatcgac cttcagccag tgaattgttg gagcatgctt ttgtgaagtg 2400
tgctgcacct ttggaaagac ctattctggg tcctgaagca tcagaccctc atcctgcaat 2460
tacacaggga acaaaaactc tgatagactt gcttgacatc catattccaa gaaatatatc 2520
ttgccctgtc tctcccattg gaagtccact tttgaggtca agatcaccac agcacatgaa 2580
tggaagaatg tcaccttctc caatatctag tccacggact gcttctggtg catcgacgcc 2640
tctcacaggc ggcaacggtg ccattccatt cagcaaccaa ttaaaacagt cagtttactt 2700
tcaagagggt cttgtaagca tgcccaagtc cccaaatggt gtctacatca gtggctctgc 2760
acataatgac tcaaatgttg acatttttcg tggaatgcaa atggggtctc acattacctc 2820
agaactagtt cccagtgaaa atgatattct gggtaagcag ttcacaaggc catctcatcc 2880
tgagccatat gattttcaat cagtcttggc ggatcgtgtt ggccggcagc tgctgcggga 2940
ccacgtgaag attaacccat cccttgatct aaatcccaac tccactttgc tgagccgagg 3000
aaatggtttg taatatcagg aattgtcctc gtcgacccac ttaaccaagc agtgcatgaa 3060
atttctaaga atacttttgg tgtacaaata agaagccaaa agcacaagag aatttttgta 3120
aataaaattg gacttgggag tagactgaaa attggggcac aaaagttcat tttgcatggc 3180
ttcaggctca ggtttctgga attcaaccct cagatatgca ttgccaaaca cagtaacagc 3240
atgaaaactt ctgacatctg atttctcaat atcaacttac caaagtgctc gatttattgg 3300
ccgagaggaa cttgtagttt tttgtataac atgagatgca gcatcaagct tgagcagcca 3360
ggagcttgga tttctcttct gcacttagac actagaaatg acagcgttca tgcaatgaca 3420
aatgtatctt ttggaacatc caagaaaaag aaaaagaaag gttgaattga tacaaaactt 3480
aacttgtaga ctattttttg ttgtacagat gatgggaaag tgtattgtga ttatgcattt 3540
aattgaagaa gagaaagtca aggatttgta aagcactgag cacatgtttt attgcagcct 3600
caattgtcat ctggatctgt tgtataaatt attactcaca gaaattgaaa tcttagtatg 3660
tgtttatggg tggggtgttg cactcgaaac tgtatacatt ccaactcaaa gacacctctc 3720
tcaccttgct tacctggttg tttctagaca tgtgtcttgt tacataaatt acacattagt 3780
ttctgttaac tagat 3795
<210> 28
<211> 3859
<212> DNA
<213> 蔓花生(Arachis duranensis)
<400> 28
aaattccgaa attttcacaa caaatcactg caagatcatt ctccattcct tgcattttgg 60
ggttttctgg gtctttttct tgttctcgct gtgctctgaa cggttcagga aattttctca 120
ctcagatttg agctgaatat tacactgtgg ggggcttcag ttcagattcc tggacttgtt 180
taactgaagt ttaatgcaat aatttccaat aactaatttc tatctctcgc cctaaagtta 240
ctgctttcgg aacttgtcag cactctattt gtgttaaggc ttatggctgg attatgatat 300
ttagaactat ggtcactgat gtgacatgat agattttttt gaagtattat ttttgggatc 360
cttttgagaa acttacttga catctcaaca taacaaatat gcctatacgg tgggcaaatc 420
cgttctcaaa ttcaaaagaa accaagaaga aagcaagtaa ggaaagtttt attggcacac 480
tgcatcggaa atttaaaaca ccatctgaag gtagattagg cagtagatct ggaggtcccg 540
gtagacgctg caatgacaca atttcagaga tgggggatcg ttctccagct gaatcaagat 600
ctccttcgcc ttccaaagtg gcaagatgtc aaagtttcgc tgaaaggcct catgctcagc 660
cattaccact tcctggtctt cacccttcaa gtataggccg agtagattct gaaattagca 720
tatcatcaaa atcaagactg gaaaagagct ccaagccatc attccttcca cttccaagac 780
ctgcatgtat gcgaggaagg acaaatcctt cagatttgga tggagatatg gttgcttcaa 840
tctccagtga tggttctgct gatagtgatg accaaacaga gtcacgcaac cgtagtcctt 900
tggcaactga caccgatact ggaactagaa cggctgccga gagtccttcc agcatgatgc 960
tcaaggatca accatctgtt gtctcccaac taaattcaag agaagccaaa aaaccagtga 1020
acattctaag taatcatatg tcttcaactt caccaaaaca gaggccttta aggaaccatg 1080
ttccgaatct gcaagttccc cctcatggtg ccttctatag tgctccagac agttccttgt 1140
caagcccatc gagaagcccg ttgagagcgt ttggcacaga tcaggtgttg aactctgcct 1200
tctcggctgg aaagccatac ccggagttca atttagccgg ttctggccac tgctctagtc 1260
caggttccgg tcacaattct gggcacaatt caatgggtgg ggacatgtca ggacagttat 1320
tttggcaacc aagcaggggt agccctgagt attctccagt tcctagtccc agaatgacta 1380
gtcctggtcc tagctctaga attcagagtg gagccgtcac acctattcat cctagagctg 1440
ggggaacacc cactgaatca cagacaagct gggctgatga tggaaaacaa cagagtcatc 1500
gtttgccact tcctcctttg acaattacca attcctcgcc tttctctcat tccaattctg 1560
cagcaacttc accatccatg ccaagaagtc cagctagagc ggaaaatcca gtgagccctg 1620
gctcacgttg gaaaaaagga aagctgattg gcagaggcac atttggacat gtctacgtcg 1680
gcttcaataa tgatagtggt gaaatgtgtg caatgaagga ggttaccctg ttttcagatg 1740
atgccaagtc tttagagagc gctaaacaat taatgcagga aatttcttta ttaagtcggc 1800
ttcggcatcc aaatattgtg cagtattatg gttctgaaac ggtagatgac aagctttaca 1860
tatacttgga gtatgtatct ggaggctcca tatataaact tcttcaagaa tatggacagt 1920
ttggtgaact agctattcgt aattatactc aacaaatttt gtcagggctt gcttatctgc 1980
atgctaagaa tactgtccac agggacatca agggtgcaaa tatacttgta gatccaaacg 2040
gccgggtcaa gttggcagat tttggcatgg caaaacatat aaaagggcaa tcatgtccat 2100
tgtcaatcaa gggaagccct tattggatgg ctcctgaggt tataaagaac actagtggat 2160
gcaaccttgc tgtggatata tggagtcttg gatgcacagt tttggaaatg gctacaacca 2220
agcccccttg gagtcaatac gaaggggttg ctgccatgtt caagataggt aatagcaagg 2280
aactcccaaa aatcccagat cacctctcaa atgaaggaaa agattttgta aggagatgtc 2340
tacaacgtga tccacatgat cgaccttcag ccagtgaatt gttggagcat gcttttgtga 2400
agtgtgctgc acctttagaa agacctattc tgggtcctga cgcatcagac cctcatcctg 2460
caattacaca gggaacaaaa actctgatag acttgctcga catccatatt ccaagaaaca 2520
tatcttgccc tgtctctccc attggaagtc cacttttgag gtcaagatca ccacagcaca 2580
tgaatggaag aatgtcacct tctccaatat ctagtccacg gactgcttct ggtgcatcga 2640
cgcctctcac aggtggcaac ggtgccattc cattcagcaa ccaattaaaa cagtcagttt 2700
actttcaaga gggtcttgta agcatgccca agtccccaaa tggtgtctac atcagtggct 2760
ctgcacataa tgactcaaat gttgacattt ttcgtggaat gcaaatgggg tctcacatta 2820
cctcagaact agttcccagt gaaaatgata ttctgggtaa gcagttcaca aggccatctc 2880
atcctgagcc atatgatttt caatcagtct tggcggatcg tgttggccgg cagctgctgc 2940
gggaccacgt gaagattaac ccatcccttg atctaaatcc caactccact ttgctgagcc 3000
gaggaaatgg tttgtaatat caggaattat cctcgtcgac ccacttaacc aagcagtgca 3060
tgaaatttct aagaatactt ttggtgtaca aataagaagc caaaagcacg agagaatttt 3120
tgtaaataaa attggactcg ggagtagact gaaaattggg gcacaaaagt tcattttgca 3180
tggcttcagg ctcaggtttc tggaattcaa ccctcagata tgcattgcca acacagtaac 3240
agcatgaaaa cttctgacat ctgatttctc aatatcaact taccaaagtg ctcgattcat 3300
tggctgagag gaacttgtag ttttttgtat aacacgagat tcagcatcaa gcttgagcag 3360
ccaggagctt ggatttctct tctgcactta gacactagaa atgatagcgt tcatgcatga 3420
caaatgtatc ttttggaact tccaagaaaa agaaaaagaa aggttgaatt gatacaaaac 3480
ttaacttgta gactattttt tgttgtacag atgatgggaa agtgtattgt gattatgcat 3540
ttaattgaag aagagaaagt caaggatttg taaagcactg agcacatgtt ttattgcagc 3600
ctcaattgtc atctggatct gttgtataaa ttattactta cagaaattga aatcttagta 3660
tgtgtttatg ggttggggtg ttgcactcga aactgtatac attccaactc aaagacacct 3720
ctctcacctt gcttacctgg ctgtttctag acgtgtcttg ttacataaat tacacattag 3780
tttctgttaa ctagattaca atatgagtta catttcctga ggttggggtg attgcattcg 3840
actagtaatt ttcatactt 3859
<210> 29
<211> 3631
<212> DNA
<213> 绿豆(Vigna radiata)
<400> 29
ggtttttgaa atattttcaa ataactgatt cctgtctctc taaaggtgtt gctttcgaat 60
ttgtgattct cctactttta aaggtttgat ggctgcatca tggctttggt aactctgtct 120
actgatgtga aatgatagat ttttttaact attactggga tccttttaag aatttgcttg 180
gcacatttca atttataaaa tacaaatatg cttacatggt ggggaaaatc atcatcaaaa 240
gaaagcaaga agaaatcaaa taaggaaagc ttttttgaca cactgcaccg gaaattaaaa 300
atttcatctg aaggtaaact aagcactaga tctggaggat cccgtagaaa ttgcaatgac 360
acagtttcag agaaggggga tcgatctcca actgaatcaa gatcaccttc accttccaaa 420
gtgggcaggt gtcaaagttt cattgaaaga cctcatgctc agccacttcc tcttcctggt 480
ctgcaccctt caagtgtagg ccgagtagat tctgaaatta gcatatcatc aaaatcaaga 540
ttggaaaagg tctccaaacc atcattgttt cttccacttc caacacctgg atgcatccgt 600
tgtaagccaa acccagcaga tttggatgga gatatgatca ctgcttcagt ctttagtgat 660
tgctctgctg acagtgatga gctacactca cacaatcgta gtcctctagt ggctgactgt 720
gagactggga tcagaactgc tgctggcagt ccttccagct caatgcccaa ggatcagcca 780
gttgtcgctt cccagctcaa ttcagcagga gtaaaaaaac caggaagtat tctgagtaat 840
catacatctt ctacttcacc aaaacggagg cctttacgca accatgttcc aaatcttcag 900
gttcctcctc acggtgcctt ctacagtgct ccagatagtt ccttgtcaag tccatcaaga 960
agtccactga gagcatttgg cacagatcag gtgttgaatt ctgctttttg ggccggaaag 1020
ccatattcag aggtcaactt tgttggatct ggacattgct ctagtccagg ttcaggtcac 1080
aattctggac ataattcgat gggaggggac atgtcaggac ctttattttg gcaaccaagc 1140
aggggtagtc cagagtattc tcctgtacct agtcccagaa tgactagccc tggtcctagc 1200
tctagaattc agagtggagc tgtcacacct attcatccca aagccggggg aacacccatt 1260
gaatcacaga caggaaggtt tgatgatggg aaacagcaga ctcatcgttt gccacttcct 1320
cctttgtcag tctccaattc ctcaccattc tctcattcaa attctgcagc aacatctccg 1380
tctatgccaa gaagtcctgc tagagcagat aatccaggct ctggctcacg ttggaagaaa 1440
ggaaagctgc ttggtagtgg ctcatttgga catgtctatc ttggcttcaa tagtgaaagt 1500
ggcgaaatgt gtgcagtgaa ggaggttacc ctgttttcag atgatcccaa gtcaatggaa 1560
agtgctaagc aatttatgca gaacccattt aaaaattact ggcaggaaat tcatttatta 1620
agccgtttac agcatccaaa tattgtccgg tattatggtt ctgaaacagt tgatgacaag 1680
ctttacatat accttgaata cgtgtctggt ggctccatac acaaacttct tcgagaatat 1740
gggcagtttg gagaactagt tattcgtagc tatactcaac aaattttgtc agggcttgct 1800
tatttgcatg ctaaaaatac tctccatcgg gacatcaaag gagcaaatat actggtagat 1860
ccaactggtc gggtcaagtt ggcagacttt ggcatggcaa aacatataac agggcaatcg 1920
tgtccattat cattcaaggg aaccccttac tggatggctc ctgaggttat aaagaactct 1980
aatggatgca atcttgcagt agatatatgg agtcttggat gcacagtttt ggaaatggct 2040
actaccaaac ctccttggtt tcagtatgaa ggggttgctg ccatgttcaa gattggtaat 2100
agcaaggaac tcccaacaat accagatcat ctctcaaatg aaggaaaaga ttttgttagg 2160
aaatgtcttc agcgtaaccc atatgatcgc ccttcagcga gtgaattatt ggaccaccct 2220
tttgtaaaac ttgctgcacc tttggaaaga cctattctgg ttcctgaagc aatggatcct 2280
gtttctggga ctacacaggg agcaaaacct ctgatgcatg ttatgccgat gcatgttatg 2340
ccgatgcatg ttatgccgat gcatgttatg ccaatgcatg ttatgccgat gcatgttatg 2400
tcgatgcatg ttatgctgca tgttgaaatc catattccaa agaatatatc ttgccccgta 2460
tctcccattg gaagcccact tttaaggtca agatcaccac agcacaggaa tggtaaaatg 2520
tctccttctc ccatatccag cccacgaact gcttccgggg catctacacc tctcgctggt 2580
ggcagtggtg ccattccttt tggtaatcac tctaaacagt taatttactt tcaagagagt 2640
tttggaagca tccctaagtc ctcaaatggt gtgtacgtta atggccctgc tcatcatgac 2700
tcaactgttg acatttttcg aggaatgcaa atagggtctc acattcaatc agagctggtt 2760
tccactgaaa atgatgttct ggttaagcag tttgcaaggc ctcctcatgc agagccatat 2820
gattttcagt cagtcttggc agatcgtgtt ggccggcagc tgctgcgtga acatgttaag 2880
attaacccat cccttgatct cagtcccaac tcatctttgc taagccgccc aaatggttta 2940
tgacatcacg atcttttcct gggccactca tctgagcagc tgttgaagtt tctcagaata 3000
ccttttggtc atgacgatat gttcattgtt ctgtttgcga aaatataatt atacaataag 3060
gagccaaagc acaagagaat cactgtaaat aactttgcga cggagaatag gctgcaccgg 3120
gcaccatagt gcattttgca tggcttcagg ctcaggctcg tggagttcat ccatttgtgc 3180
cacagcatta aactttctga caatttgtcc atatcaagca atcaactcac taatctcact 3240
tgttggcaaa gaagaacttg ttattgtatg taacatattt aagatgctgc atctgcatga 3300
gatgtttctc tcctgcactt gagatactat aaatgacaga gttcgtgcaa tgacaaatgc 3360
atctcctgga attggaacac cagagaaatg gttaaaattg atacaaaact taacttgtag 3420
actattttct ttttggagga tagacattgt tgtacagtag ataggaaact gtattgtgat 3480
tattctgaat ttagggatgg agacctcgat tacattgtgg gcttaaatgt attattgtca 3540
ttgcgtactc tactactgga tctgttctgc tctgtacatc attgttcaac agcaaaatgg 3600
aagaaatatc atcttatcat tggttaatac a 3631
<210> 30
<211> 3922
<212> DNA
<213> 车轴草(Trifolium pratense)
<400> 30
atgctcaagg atcaaccgtc tgctgtttca caagtgaatt cgagagaagt caaaaaatcg 60
gctaacattc taagtaatca gacaccttct acttcaccaa aacggaggcc attacgtcac 120
catgttccaa atcttcaggt tccgccccat ggtatcttct atagtggtcc cgacagttcc 180
ttgtcaagtc catcaaggag tccattgagg gcatttggta cagatcaggt gttgaactcc 240
gctttttggg ccggaaagca atatccagag gtcaatttca ttggatctgg acactgctct 300
agtccaggtt ctggtcacaa ttcagggcat aattcaatgg gaggggacat gtcgggacca 360
ttattttggc aaccaagcag gggtagccct gagtattctc cggtacctag tcctagaatg 420
actagccctg gtccaagctc aagaattcag agtggagcag tcacacctat tcatcctaga 480
gccgctggaa caccgactga atcacagaca ggacgggttg atgatggaaa acaacagagt 540
catcgtttgc cactccctcc tttaacagtt aacaatacct cgcccttctc tcattctaat 600
tctgcagcaa catctccatc tatgccaaga agtccagcta gagcagatag tccaatgagc 660
tctggatcac gttggaaaaa aggaaagctg cttggcagag gcacatttgg acacgtctat 720
attggcttca ataggtgtga acttttgata aaatgttcaa aatttcatta tggttctttt 780
tttaattttt tttttctttc tatatatgca tcttaacaat aattatttgg ttaaaaatgt 840
ttgtctcttc tagtgaaagt ggtgaaatgt gtgcaatgaa ggaggttact atgttttcag 900
atgatgctaa gtctatggaa agtgctaagc aattaatgca ggtaacttga cacctaaatc 960
tagcaaaatt tcatgctggt taatatttca ttttcattag gagcaacatt atattttcta 1020
gtcttgaatt taacacagct ttcgaaatct aactacaaga tttttgacag gaaattcatt 1080
tattgagccg tttaaggcat ccaaatattg tgcagtatta tggttctgaa acagtaagtt 1140
atttccttcc tttcctgtgt acaaaaccca tactgagaaa caattttagc attcagattc 1200
ttaaattcac gttatctgct ggtgaatgat gatgattttt attttggatt ctgttataga 1260
ccgttcattt ctaatgtatg catgaccttc tagtcacatg actgtagttt ctggacgttt 1320
acctactaat gatcaatata gtagtagaaa tttgttagca atatttgttt ttaattttta 1380
tagcttactt atagaagttt ttaatcatcc cttgtacagg tagatgacaa gctttacata 1440
taccttgagt atgtatctgg aggctccata cataaacttc ttcaagaata tggacaattt 1500
ggcgaactag ctattcgaag ttatactcaa caaattttgt cagggcttgc ttttttacat 1560
gctaaaaata ccctacacag gtgaactaag gcatctatta ctaatttcaa tttcaatgtt 1620
ttaaattttg ctgctgccac acaatggttt gttttctttt ctttctattt ttaaaaactt 1680
ctgatatatc tttctctgta attccatgta catgtatagg gatatcaaag gagcaaacat 1740
actggtagat ccaaatggtc gggtcaaggt tgcagacttt ggcatggcaa aacatgtaag 1800
cttagtagag atgataaccg tccagtatcc attacatctg tatttaagct gaatggagtt 1860
agatatgaag attagattct aatgcatatt gaaatcatta atcttagttc aaaagtcaat 1920
aatttactag atcaagaaag ttgttatttc caatttcatg agattgtaca ccattgcttt 1980
tcccccccaa atgttcctta taatcggttt cccccatatt tttgaagatc atgtccaccc 2040
ctcgttatgc ttcgcctcct ctgtctctaa tattcttcta ataaaaaaat cagtacattc 2100
tgatgaaata ttgatgtttc tggctttctt agtagcaatt agtcaattaa aaatgttata 2160
aaaaaaatgg gaagatggaa ccaaacccta tttataagta tgcaaagaga aaccacgagt 2220
aatgaaaaaa ggtgatggca tgatttacct tgtagatcat gttccatatt tgtccaaaac 2280
attgcatgat ttatcctgtc tgttgtactt atacatttca tgatttgcag ataacaggac 2340
aatactgtcc attgtcattc aaaggaagtc cttattggat ggctcctgag gttctacttt 2400
tatttttttg ctgttaagtt ctttagattt taattcagta ttagctttgt cactcactat 2460
attttctttt tgacgattca ggttataaag aactccaagg aatgtagcct tggtgtggat 2520
atatggagtc tcggttgcac agttttggaa atggctacaa ctaaaccgcc ttggtctcag 2580
tatgaagggg ttagtaattc atgtatatac actggatttt atatttacaa agatctaata 2640
atatttcttg tattataaac atttcaactt tttaaattat ttcctcttac tgcttgatgt 2700
atttacgtgt ttctataaca ggttgcagca atgttcaaaa ttggcaacag caaggagctc 2760
ccaacgatcc ccgatcatct ctcagatgca ggaaaagatt ttgttagaaa atgtctacaa 2820
cgtaatccac gtgatcgccc ttcagcaagt gaattattgg accacccttt tgtaaaaggt 2880
gctgcacctt tggaaagacc tattatggtt cctgaagctt cagaccctgt ttctggaatt 2940
acacatggaa caaaagctct ggtgcgtaac ctatttacta caaaaattat ttgaaactcg 3000
ttacacatgg agttcagttt tcttgtatca atgagttagc tttgtactat tcaatctgta 3060
ttccattgtt tttttttaat aattgcataa tctttgatca attctcaggg cattggacaa 3120
ggaagaaatc tgtctgcgtt ggattcagat aaactcttag ttcattcttc tagggttttg 3180
aaaagtaatc ctcatgaaag gtatctctct ctttctctcc cacnnnnnnn nnnnnnnnnn 3240
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300
nnnnnnnnnn nnnnnnnntg cgcgtgcgcg cacacacaca cacaaatgca tgcgaggctg 3360
ctgatattgc caatggtaat ctgacattat ttcattaatt tccagtgaaa accatattca 3420
taggaatata tcttgcccgg tctctcccat tggaagcccg cttttgaggt caagatcacc 3480
acatcagagg agtgggagat tatcaccttc tcctatatct agccctcgga ctgcttctgg 3540
tgcatccacc cctctcactg gcggcagtgg tgccattcca tttaatcact taaaacaggc 3600
agcttacttt caagaatgtc ttggaagcat gccaaaatcc tcaaacggtg tctacatgaa 3660
cggctctact caccacgacc caaatattga catttttcgg ggaatgcaaa ccgggtctca 3720
cattaaatca gaacttgtgt ccagcgaaaa cgatgctctc agtaagcagt ttgtaaggtc 3780
tcctcatgca gagccatatg actttcagtc tgtcttagct gatcgtgttg gccggcagct 3840
tctgggagat catgttaaga ttaacccatc atttgatccg agtcccagct cgtcgatgct 3900
ccaccgaaca aatggtttat ga 3922
<210> 31
<211> 4857
<212> DNA
<213> 紫花苜蓿(Medicago sativa)
<400> 31
atgcctacat ggtggggaaa atcatcatca aaagaaacca agaagaaagc aggtaaggaa 60
agtattattg acacattgca cagaaaattt aaatttccat ctgagggtaa actaagcact 120
atatctggag gatctcgtag acgaagcaat gacacaattt cagagaaggg ggaccgttct 180
ccatctgaat caagatcccc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggcctcatg ctcaacccct tccacttccg ggtttgcacc cttcaagttt aggtcgagtt 300
gattctgaaa ttagtatatc agcgaaatcg agactggaaa agccctccaa gccgtcgtta 360
tttcttccac ttccaaaacc ttcatgcata cgttgtgggt caactcctgc agatttagat 420
ggagatatgg tcaatgcttc agtcttcagt gattgctctg ctgatagtga tgagccagca 480
gactcacgca accgtagtcc tctggcaact gactcggaaa ctggtacaag aactgctgcc 540
ggcagtcctt ccaggttgcc tcctcaaacc ataacacgct tccacattgt acatgttatt 600
gcatatgcat gccttaatta tttgtgattg attgcattag aaatatgtat cttttcaact 660
tcactcaaaa gtttccctgt ttctgaagct tggtgctcaa ggatcaatca tctgctgttt 720
cccaaccgaa ttcgagagaa gtaaaaaaaa cggcaaacat tctcagtaat cacacacctt 780
ctacttcacc aaaacggaag ccattacgcc accatgttcc aaatcttcaa gttccacctc 840
atggtgtctt ctatagtggt cccgacagtt ccttgtcaag tccatcaaga agtccgatga 900
gggcatttgg tacagatcag gtgttgaact ctgctttttg ggctggaaag ccatatccag 960
agatcaattt tgttggatct ggccactgct ctagtccagg ttctggtcac aattctgggc 1020
ataattcaat gggaggggac atgtcaggac cattattttg gcaaccaagt aggggtagcc 1080
ctgagtattc tccaatacct agccctagaa tgactagccc tggtccaagc tcaagaattc 1140
agagtggagc agtcacacct attcatccta gagcaggggg aacaccaact gaatcacaga 1200
caggacgggt tgatgatgga aaacaacaaa gtcatcgttt gcccctccct cctttaacag 1260
ttaccaatac ctcgccattc tctcattcta attctgcagc aacatctcca tctatgccga 1320
gaagtccagc tagagcagat agtccaatga gctctgggtc acgttggaaa aaaggaaagc 1380
tgcttggcag aggtacattt gggcacgtct atattggctt caataggtat gtgccctgga 1440
taaaatgtaa acatttcgtc atttttttat gattaatttc tatttatgca tcttaaaaat 1500
actgatgtgg ttaaaaaatg tttgtctctc ttagtcaaag tggtgaaatg tgtgcaatga 1560
aggaggttac tctgttttca gatgatgcca agtctttgga aagtgctaag caattgatgc 1620
aggtatactg ttcccttcta aatctagcaa aaacttcata ctggtaaaca gttcattttc 1680
gttaggtgca atactgtatt ttctagtctt gaattcaaca cagctttcaa aatctaacta 1740
caagatttct gacaggaagt tcatttatta agccgtttaa gacatccaaa tattgtgcag 1800
tattatggct ctgaaacggt aagttctctc cttcgatctg atggcaaatg acgacaattt 1860
attttttatt ttattataga ctgtgcattt ttaatggatg catgatcttc tagtcacatg 1920
catattagtt agtttgtgga agtttaccta acaagaacaa tatagcagta gaaatttgtt 1980
agcattattt gtttctaaag tctacttata gaagtttttg gatcatccct tgcacaggtt 2040
gatgacaagc tttacatata ccttgagtat gtatctggag gttccataca taaacttctt 2100
caagaatatg ggcaatttgg tgaactagct attcgaagtt atactcaaca aattttgtca 2160
gggcttgctt atttacatgc taaaaatact cttcacaggt gaactaaggc atcttttcat 2220
aatctcaatg ttttaaattt tgctgctgcc acatatttag tttctttttt aaacttctga 2280
catatctttc tctgaaattc catgcacatg tttagggaca tcaaaggagc aaacatattg 2340
gtagatccaa atggtcgggt caaggttgca gactttggca tggcaaaaca tgtaagctta 2400
gagatgataa ctgtccagtt tccattatat tttcatttag ctgaaaatta gatatgaaaa 2460
ttagattctt tttttttttg acaaaaaaaa attagattct aatgcttatt gaaattataa 2520
atcataggtt ttttttctaa aaaatctcaa tatatcgatg tttctggctt cttgatagca 2580
attgggcgat ttaaaatgtt aaaagaaaat gtgaagatgg aaccctattt ttaagtatgg 2640
aaagagaaac cagaataata caaaattgtg atggtgtatg atttaccttg taaaccacga 2700
tttatgctgt ccaggacgtg catgatttgt cccatttgtt gtacttacaa ttacatgatt 2760
cacagataac agggcaatac tgtccattgt cattcaaagg aagtccttat tggatggctc 2820
ccgaggttct actttttctt ttttgggttc agttctttag attttaattg cattttagtt 2880
ttgtcactca ttatcttttc tttttgatga ttcaggttat aaagaactcc aaggaatgca 2940
gccttggtgt ggatatatgg agtcttggat gcacagtttt ggaaatggct acaacgaagc 3000
ctccttggtc tcagtatgaa ggggttagta attcatgtat aatacatgag gttttatatt 3060
tacaaagacc tggtgatatt tttgtattat aaacatgtca acttttaaat taatttacct 3120
gttactataa caggttgctg ccatgttcaa aattggtaac agcaaggagc tcccaacaat 3180
tccagatcat ctctcaaatg aaggaaaaga ttttgttaga aaatgtctac aacgtaatcc 3240
acgtgatcgc ccttcagcaa gtgaattatt agaacaccct tttgtaaaag gtgctgcacc 3300
tttggaaaga cctattatgg ttcccgaagc ttcagaccct attactggaa ttacacatgg 3360
aacaaaagct ctggtgcgta acctatttcc tactccaatc gttggaaact cacttcagtt 3420
gtgtttgtgt ggttgttcat gttaccattc gatggtactt tgtctttcat tttatgcgaa 3480
tatcaaactg tcgcattgcc ttatctacac tatagaacaa aataggggac agtttgctct 3540
cttcatgaaa atgtcatgtg aacttctgtt cttgttttca catttctcat ggttgagtaa 3600
tttaagtggc gtgtaaattt tataggctat tcacgctctt agtaatacat aaatgtaaag 3660
attttgatcc gtcaagtcca aaatatcgag tcaattgcgt gatctatatt ttcattcctt 3720
tttctgtgta agttattaag gaaattgttc tttgtatgag gcagcatgaa tgatgcataa 3780
gggaaaaaca tagatgatag ctcaatacat agtaggttga gggatagtga gatattggtt 3840
ggaaagcgat aaagttctag ggtttttaaa ttggtttccc ttttcatcag atctggctag 3900
aattttatgt caagagtaag tatacacatg gagttcagtt aggatggaga cttttgttat 3960
ttcaattcag tgtaatttat tttaaaagta atataaatct tgtttcgtgt ttattaggat 4020
gaagtatctt ccaaatatga ggtcacaaca agtttttctt gcatcaatga gttagctttg 4080
tactactcaa tctgtattct cttgttttat ttttataata attgcatcaa tttcagggta 4140
ttggacaagg aaggaatctg tctgccttgg attcagataa actcttggtt cattcttcta 4200
gggttttgaa aaataatcct catgaaaggt atctctctag ctcactcacc cacgcgcaca 4260
cacacacaca cacaaaaatg catgcaaggc tgttgacatt gccaatggta atctgaaatt 4320
atttcatcaa tttccagtga aatccatatt caaaggaata tatcttgccc agtctctccc 4380
attggaagcc cacttttgag gtcaagatca ccacagcaga ggagtgggag actgtctcct 4440
tctcctatat ctagccctcg gaccgcttct ggtgcatcca cgcctctcac tggcggcagt 4500
ggtgccattc cattcagtaa ccacttaaaa caatcagttt actttcaaga gtgtctcgga 4560
agcatgccga aatccccaaa tggtgcctac attaacggct cttctcacca cgattcaaat 4620
atcgacattt ttcaaagaat gcaagccggg tctcacatta aatcagaact agtctccagt 4680
gataatgatg ctcttggcaa gcagttcgta aggtctcctc atgcagaacc gtatgacttt 4740
cagtcggtct tagctgatcg tgttggccgg cagcttctgg gggatcatgt taagattaac 4800
ccgtcatttg atccaagtcc cagctcatcg atgctcaacc ggacaaatgg tttatga 4857
<210> 32
<211> 4796
<212> DNA
<213> 紫花苜蓿(Medicago sativa)
<400> 32
atgcctacat ggtggggaaa atcatcatca aaagaaacca agaagaaagc aggtaaggaa 60
agtattattg acacattgca cagaaaattt aaatttccat ctgagggtaa actaagcact 120
atatctggag gatctcgtag acgaagcaat gacacaattt cagagaaggg ggaccgttct 180
ccatctgaat caagatcccc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggcctcatg ctcaacccct tccacttccg ggtttgcacc cttcaagttt aggtcgagtt 300
gattctgaaa ttagtatatc agcgaaatcg agactggaaa agccctccaa gccgtcatta 360
tttcttccac ttccaaaacc ttcatgcata cgttgtgggc caactcctgc agatttagat 420
ggagatatgg tcaatgcttc agtcttcagt gattgctctg ctgatagtga cgagccagca 480
gactcacgca accgtagtcc tctggcaact gactcggaaa ctggtacaag aactgctgcc 540
ggcagtcctt ccaggttgcc tcctcaaacc ataacacgct tccacattgt acatgttatt 600
gcatatgcat gccttaatta tttgtgattg attgcattag aaatacgtat cttttcaact 660
tcactcaaaa gtttccctgt ttctgaagct tggtgctcaa ggatcaatca actgctgttt 720
cccaaccgaa ttcgagagaa gtaaaaaaaa cggaaaacat tctcagtaat cacacacctt 780
ctacttcacc aaaacggaag ccattacgcc accatgttcc aaatcttcaa gttccacctc 840
atggtgtctt ctatagtggt cccgacagtt ccttgtcaag tccatcaaga agtccgatga 900
gggcatttgg tacagatcag gtgttgaact ctgctttttg ggctggaaag ccatatccag 960
agatcaattt cgtcggatct ggccactgct ctagtccagg ttctggtcac aattctgggc 1020
ataattcaat gggaggggac atgtcaggac cattattttg gcaaccaagt aggggtagcc 1080
ctgagtattc tccaatacct agccctagaa tgactagccc tggtccaagc tcaagaattc 1140
agagtggagc agtcacacct attcatccta gagcaggggg aacaccaact gaatcacaga 1200
caggacgggt tgatgatgga aaacaacaaa gtcatcgttt gcccctccct cctttaacag 1260
ttaccaatac ctcgccattc tctcattcta attctgcagc aacatctcca tctatgccga 1320
gaagtccagc tagagcagat agtccaatga gctctgggtc acgttggaaa aaaggaaagt 1380
tgcttggcag aggcacattt ggacacgtct atattggctt caataggtat gtaccctgga 1440
taaaatgtaa acatttcatc attttttttt tatgattaat ttctatttat gcatcaacaa 1500
tacttatgtg gttaaaaaat gtttgtctct cagtcaaagt ggtgaaatgt gtgcaatgaa 1560
ggaggttact ctgttttctg atgatgccaa gtctttggaa agtgctaagc aattgatgca 1620
ggtatacttt tcccttctaa atcttgcaaa atttcatact ggtaaacagt tcattttcgt 1680
tggtgcaatg ctatattttc tagtcttgaa tttaacgcag ctttcgaaat ctaactacaa 1740
gatttctgac aggaagttca tttattaagt cgattaagac atccaaatat tgtgcagtat 1800
tacggctctg aaacagtaag ttctctcctt cactcgatct gatggcgaat gatgacgatt 1860
attttttatt ctattataga cggtctattt ttaatggatg catgatcttc cagtcacatg 1920
aatattagtt agtttctgga agtttaccta acaatgaaca gtatagttgt agaaatttgt 1980
tggcattatt tgtttctaaa gcctacttat aaaagttttt ggatcatctc ttgcacaggt 2040
agatgacaag ctttacatat accttgagta tgtatctgga ggttccatac ataaacttct 2100
tcaagattat gggcaatttg gtgaactagc tattcgaagt tatacccaac aaattttgtc 2160
agggcttgct tatttacatg ctaaaaatac tcttcacagg tgaactaagg catctattca 2220
taatttcaat gttttaaatt ttgctgcggc cacataatga tttgtttctt ttttaaactt 2280
ctgatatata tttctctgaa attccttgca cgtgtttagg gacatcaaag gagcaaacat 2340
attggtagat ccaaatggtc gggtcaaggt tgcagacttt ggcatggcaa agcatgtaag 2400
cttagagatg ataactgtcc agtttccatt atatcttcat ttaagctgac ttagctagat 2460
atgaaaataa gattctaatg cttattgaaa ttataaatct taggtttttt ctaataaaat 2520
ctcaatatat cgatgtttct ggcttcttga tagcaattgg gtgatttaaa atgttaaaag 2580
aaaatgtgaa gacggaaccc tatttttaag tatggaaaga gaaaccatga ttaataaaaa 2640
attgtgatgg tatgatttac cttgtaaacc atgttctatg ctgtccagga cgtgcatgat 2700
ttgtcccatt tgttgtactt ataattacat gatttgcaga taacagggca atactgtcca 2760
ttgtcattca aaggcagtcc ttattggatg gctcctgagg ttctattttt ttcttttttg 2820
ggttcagttt tttagatttt aattgcattt tagttttgtc actcaatatc tttccttttt 2880
gatgattcag gttataaaga actccaagga atgcagcctt ggtgtggata tatggagtct 2940
tggatgcaca gttttggaaa tggctacaac gaagcctcct tggtctcagt atgaaggggt 3000
tagtaattca tgtataatac atgagatttt atttttacaa agaccttgcg atatttttgt 3060
attataaaca tgtcaacttt ttaaattaat tcttcttact gcttggtgta ttttcgtgtt 3120
tctataacag gttgctgcca tgttcaaaat tggtaatagc aaggagctcc caacaattcc 3180
agatcatctc tcaaatgaag gaaaagattt tgttagaaaa tgtctacaac gtaatccacg 3240
tgatcgccct tcagcaagtg aattattaga ccaccctttt gtaaaaggtg ctgcaccttt 3300
ggaaagacct attatggttc ccgaagcttc agaccctatt tctggaatta cacatggaac 3360
aaaagctctg gtgcgtaacc tatttcctac tccaatcgtt ggaaactcac ttcagttgtg 3420
tttgtgtggt tgttcatgtt accatttgat ggtactttgt ctttcatttt atgcaaatat 3480
caaactgttg cattgcctta tctacacaat agaacaaaat aggggacagt ttgctctctt 3540
cattaaaatg tcatgtgaac tttctgttct tgttttcaca tttctcatgg ttgagtaatt 3600
taagtggcgt gtatatttta taggctattc atgctattaa caatacataa atgtatagat 3660
tttgatccat caagtccaga atatcgatag ctcaagacat aatttaggtt gaggggtagt 3720
gggatattgg ctggaaagcg ataaagttct aggattttta aattggtttc ccttttcatc 3780
agatctggct agaattttat gtcaagagta agtatacaca tggatttcag ttaggttgga 3840
gacttttgtt atttcaattc agtataattt caattgaaat aacaattgaa attatacttt 3900
tgttatttca attcagtata atttatttta aagtaatata aatcttgatt agtgtttctt 3960
agcacgttgt atcttccaaa tatgaggtca cagctagttt ttcttgcatg aatgagttag 4020
ctttgtacta gtcaatctgt attctcttgt tttattttta taataattgc attgatttca 4080
gggcattgga caaggaagga atctgtctgc cttggattca gataaactct tagttcattc 4140
ttctagggtt ttgaaaaata atcctaatga aaggtatctc tctagttcac tcactaacgc 4200
acatacacac acaaaaattc atgcaaggct gttgacattg ccgatggtaa tctgaaatta 4260
tttcatcaat ttccagtgaa atccatattc aaaggaatat atcttgccca gtctctccca 4320
ttggaagccc acttttgagg tcaagatcac cacagcagag gagtgggaga ttgtctccgt 4380
ctccaatatc tagccctcgg accgcttctg gtgcatccac gcctctcact ggcggcagta 4440
gtgccattcc attcagtaac cacttaaaac aatcagttta ctttcaagag tgtctcggaa 4500
gcatgccaaa atccccaaat ggtgtctaca ttaatggctc ttctcaccac gattcaaata 4560
tcgacatttt tcaaagaatg caagccgggt ctcacattaa atcagaacta gtctccagtg 4620
ataatgatgc tcttggcaag cagttcgtaa ggtctcctca tgcagaacca tatgactttc 4680
agtcggtctt ggctgatcgt gttggccggc agcttctggg ggatcatgtt aagattaacc 4740
catcatttga tccaagtccc agctcgtcga tgctcaaccg gacaaatggt ttatga 4796
<210> 33
<211> 4901
<212> DNA
<213> 紫花苜蓿(Medicago sativa)
<400> 33
atgcctacat ggtggggaaa atcatcatca aaagaaacca agaagaaagc aggtaaggaa 60
agtattattg acacattgca cagaaaattt aaatttccat ctgagggtaa actaagcact 120
atatctggag gatctcgtag acggagcaat gacacaattt cagagaaggg ggaccgttct 180
ccatctgaat caagatcccc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggcctcatg ctcaacccct tccacttccg ggtttgcacc cttcaagttt aggtcgagtt 300
gattctgaaa ttagtatatc agtgaaatcg agactggaaa agccctccaa gccgtcatta 360
tttcttccac ttccaaaacc ttcatgcata cgttgtgggc caactcctgc agatttagat 420
ggagatatgg tcaatgcttc agtcttcagt gattgctctg ctgatagtga cgagccagca 480
gactcacgca accgcagtcc tctggcaact gactcggaaa ctggtccaag aactgctgcc 540
ggcagtcctt ccaggttgcc tcctcaaacc ataacacgct tccacattgt acatgttatt 600
gcatatgcat gccttaatta tttgtgattg attgcattag agatacgtat cttttcaact 660
tcactcaaaa gtttccctgt ttctgaagct tggtgctcaa ggatcaatca tctgctgttt 720
cccaaccgaa ttcgagagaa gttaaaaaaa cggcaaacat tctcagtaat cacacacctt 780
ctacttcacc aaaacggaag ccattacgcc accatgttcc aaatcttcaa gttccacctc 840
atggtgtctt ctatagtggt cccgacagtt ccttgtcaag tccatcaaga agtccgttga 900
gggcatttgg tacagatcag gtgttgaact ctgctttttg ggctggaaag ccatatccag 960
agatcaattt cgttggatct ggccactgct ctagtccagg ttctggtcac aattctgggc 1020
ataattcaat gggaggggac atgtcaggac cattattttg gcaaccaagt aggggtagcc 1080
ctgagtattc tccaatacct agccctagaa tgactagccc tggtccaagc tcaagaattc 1140
agagtggagc agtcacacct attcatccta gagcaggggg aacaccaact gaatcacaga 1200
caggacgggt tgatgatgga aaacaacaaa gtcatcgttt gcccctccct cctttaacag 1260
ttaccaatac ctcgccattc tctcattcta attctgcagc aacatctcca tctatgccga 1320
gaagtccagc tagagcagat agtccaatga gctctgggtc acgttggaaa aaaggaaagc 1380
tgcttggcag aggtacattt gggcacgtct atattggttt caataggtat gtaccctgga 1440
taaaatgtaa acatttcgtc atttttttat gattaatttt tatttatgca tcttaaaaat 1500
actgatgtgg ttaaaaaatg tttgtctctc ttagtcaaag tggtgaaatg tgtgcaatga 1560
aggaggttac tctgttttca gatgatgcca agtctttgga aagcgctaag caattgatgc 1620
aggtatactg ttcccttcta aatctagcaa aaacttcata ctggtaaaca gttcattttc 1680
gttaggtgca atactgtatt ttctagtctt gaattcaaca cagctttcaa aatctaacta 1740
caagatttct gacaggaagt tcatttatta agccgtttaa gacatccaaa tattgtgcag 1800
tattatggct ctgaaacggt aagttctctc cttcgatctg atggcaaatg acgacaattt 1860
attttttatt ttattataga ctctgcattt ttaatggatg catgatcttc tagtcacatg 1920
catattagtt agtttgtgga agtttaccta acaagaacaa tatagtagta gaaatttgtt 1980
agcattattt gtttctaaag tctacttata gaagtttttg gatcatccct tgcacaggga 2040
gatgacaagc tttacatata ccttgagtat gtatctggag gttccataca taaacttctt 2100
caagaatatg ggcaatttgg tgaactagct attcgaagtt atactcaaca aattttgtca 2160
gggcttgctt atttacatgc taaaaatact cttcacaggt gaactaaggc atcttttcat 2220
aatctcaatg ttttaaattt tgctgctgcc acatatttag tttctttttt aaacttctga 2280
gatatctttc tctgaaattc catgcacatg tttagggaca tcaaaggagc aaacatattg 2340
gtagatccaa atggtcgggt caaggttgca gactttggca tggcaaaaca tgtaagctta 2400
gagatgataa ctgtccagtt tccattatat tttcatttag ctgaaaatta gtttcttttt 2460
tttttttgac aaaaaaaaat tagattctaa tgcttattga aattataaat cataggtttt 2520
tttttctaaa aaatctcaat atatcgatgt ttctggcttc ttgatagcaa ttgggcgatt 2580
taaaatgtta aaagaaaatg tgaagatgga acccgatttt taagtatgga aagagaaacc 2640
agaataatac aaaattgtga tggtgtatga tttaccttgt aaaccacgat ttatgctgtc 2700
caggacgtgc atgatttgtc ccatttgttg tacttacaat tacatgattc acagataaca 2760
gggcaatact gtccattgtc attcaaagga agtccttatt ggatggctcc cgaggttcta 2820
ctttttcttt tttgggttca gttcttttgg ttttaattgc attttagttt tgtcactcat 2880
tatcttttct ttttgatgat tcaggttata aagaactcca aggaatgcag ccttggtgtg 2940
gatatatgga gtcttggatg cacagttttg gaaatggcta caacgaagcc tccttggtct 3000
cagtatgaag gggttagtaa ttcatgtata atacatgaga ttttatattt acaaagacct 3060
gatgatattt ttgtattata aacatgtcaa cttttaaatt aattcttctt actgcttggt 3120
gtatttacgt gttactataa caggttgctg ccatgttcaa aattggtaac agcaaggagc 3180
tcccaacaat tccagatcat ctctcaaatg aaggaaaaga ttttgttaga aaatgtctac 3240
aacgtaatcc acgtgatcgc ccttcagcaa gtgaattatt agaacaccct tttgtcaaag 3300
gtgctgcacc tttggaaaga cctattatgg ttcccgaagc ttcagaccct attacaggaa 3360
ttacacatgg aacaaaagct ctggtgcgta acctatttcc tactccaatc gttggaaact 3420
cacttcagtt gtgtttctgt ggttgttcat gttaccattc gatggtactt tgtctttcat 3480
ttgatgcgaa tatcaaactg tcgcattgcc ttatctacac tatagaacaa aataggggac 3540
agtttgctct cttcatgaaa atgtcatgtg aacttctgtt cttgttttca catttctcat 3600
ggttgagtaa tttaagtggc gtgtatattt tataggctat tcacgctctt agtaatacat 3660
aaatgtaaag attttgatcc gtcaagtcca aaatatcgag tcaattgcgt gatctatatt 3720
ttcattcctt tttctgtgtc aattgcgtga tctatatttt cattcctttt tctgtgtaag 3780
ttattaagga aattgttctt tgtatgaggc agcatgaatg atgggaaaaa catagatgat 3840
agctcaatac atagtttagg ttgagggata gtgagatatt ggttggaaag cgataaagtt 3900
ccagggtttt taaattggtt tcccttttca tcagatctgg ctagaatttt atgtcaagag 3960
taagtataca catggagttc agttaggatg gagacttttg ttatttcaat tcagtgtaat 4020
ttattttaaa agtaatataa atcttgtttc gtgtttatta gcatgaagta tcttccaaat 4080
atgaggtcac aacaagtttt tcttgcatca atgagttagc tttgcactac tcaatctgta 4140
ttctcttgtt ttatttttat aataattgca tcaatttcag ggcattggac aaggaaggaa 4200
tctgtctgcc ttggattcag ataaactctt ggttcattct tctagggttt tgaaaaataa 4260
tcctcatgaa aggtatctct ctagctcact cacccactcg cacacacaca cacaaaaaaa 4320
aatgcatgca aggctgttga cattgccaat ggtaatctga aattatttca tcaatttcca 4380
gtgaaatcca tattcaaagg aatatatctt gcccagtctc tcccattgga agcccacttt 4440
tgaggtcaag atcaccacag cagaggagtg ggagactgtc tccttctcct atatctagcc 4500
ctcggaccgc ttctggtgca tccacgcctc tcactggcgg cagtggtgcc attccattca 4560
gtaaccactt aaaacaatca gtttactttc aagagtgtct cggaagcatg ccgaaatccc 4620
caaatggtgc ctacattaac ggctcttctc accacgattc aaatatcgac atttttcaaa 4680
gaatgcaagc cgggtctcac attaaatcag aactagtctc cagtgataat gatgctcttg 4740
gcaagcagtt cgtaaggtct cctcatgcag aaccgtatga ctttcagtcg gtcttagctg 4800
atcgtgttgg ccggcagctt ctgggggatc atgttaagat taacccatca tttgatccaa 4860
gtcccagctc aacgatgctc aaccggacaa atggtttatg a 4901
<210> 34
<211> 4861
<212> DNA
<213> 紫花苜蓿(Medicago sativa)
<400> 34
atgcctacat ggtggggaaa atcatcatca aaagaaacca agaagaaagc aggtaaggaa 60
agtattattg acacattgca cagaaaattt aaatttccat ctgagggtaa actaagcact 120
atatctggag gatctcgtag acgaagcaat gacacaattt cagagaaggg ggactgttct 180
ccatctgaat caagatcccc ttcaccttcc aaagtagcaa ggtgtcaaag ttttgctgaa 240
aggcctcatg ctcaacccct tccacttccg ggtttgcacc cttcaagttt aggtcgagtt 300
gattctgaaa ttagtatatc agcgaaatcg agactggaaa agccctccaa gccgtcatta 360
tttcttccac ttccaaaacc ttcatgcata cgttgtgggt caactcctgc agatttagat 420
ggagatatgg tcaatgcttc agtcttcagt gattgctctg ctgatagtga tgagccagca 480
gactcacgca accgtagtcc tctggcaact gactcggaaa ctggtacaag aactgctgcc 540
ggcagtcctt ccaggttgcc tcctcaaacc ataacacgct tccacattgt acatgttatt 600
gcatatgcat gccttaatta tttgtgattg attgcattag aaatatgtat cttttcaact 660
tcactcaaaa gtttccctgt ttctgaagct tggtgctcaa ggatcaatca tctgctgttt 720
cccaaccgaa ttcgagagaa gtaaaaaaaa cggcaaacat tctcagtaat cacacacctt 780
ctacttcacc aaaacggaag ccattacgcc accatgttcc aaatcttcaa gttccacctc 840
atggtgtctt ctatagtggt cccgacagtt ccttgtcaag tccatcaaga agtccgatga 900
gggcatttgg tacagatcag gtgttgaact ctgctttttg ggctggaaag ccatatccag 960
agatcaattt tgttggatct ggccactgct ctagtccagg ttctggtcac aattctgggc 1020
ataattcaat gggaggggac atgtcaggac cattattttg gcaaccaagt aggggtagcc 1080
ctgagtattc tccaatacct agccctagaa tgactagccc tggtccaagc tcaagaattc 1140
agagtggagc agtcacacct attcatccta gagcaggggg aacaccaact gaatcacaga 1200
caggacgggt tgatgatgga aaacaacaaa gtcatcgttt gcccctccct cctttaacag 1260
ttaccaatac ctcgccattc tctcattcta attctgcagc aacatctcca tctatgccga 1320
gaagtccagc tagagcagat agtccaatga gctctgggtc acgttggaaa aaaggaaagc 1380
tgcttggcag aggtacattt gggcacgtct atattggctt caataggtat gtaccctgga 1440
taaaatgtaa acatttcgtc atttttttat gattaatttc tatttatgca tcttaaaaat 1500
actgatgtgg ttaaaaaatg tttgtctctc ttagtcaaag tggtgaaatg tgtgcaatga 1560
aggaggttac tctgttttca gatgatgcca agtctttgga aagtgctaag caattgacgc 1620
aggtatactg ttcccttcta aatctagcaa aaacttcata ctggtaaaca gttcattttc 1680
gttaggtgca atactgtatt ttctagtctt gaattcaaca cagctttcaa aatctaacta 1740
caagatttct gacaggaagt tcatttatta agccgtttaa gacatccaaa tattgtgcag 1800
tattatggct ctgaaacggt aagttctctc cttcgatctg atggcaaatg acgacaattt 1860
attttttatt ttattataga ctgtgcattt ttaatggatg catgatcttc tagtcacatg 1920
catattagtt agtttgtgga agtttaccta acaagaacaa tatagcagta gaaatttgtt 1980
agcattattt gtttctaaag tctacttata gaagtttttg gatcatccct tgcacaggtt 2040
gatgacaagc tttacatata ccttgagtat gtatctggag gttccataca taaacttctt 2100
caagaatatg ggcaatttgg tgaactagct attcgaagtt atactcaaca aattttgtca 2160
gggcttgctt atttacatgc taaaaatact cttcacaggt gaactaaggc atcttttcat 2220
aatctcaatg ttttaaattt tgctgctgcc acatatttag tttctttttt aaacttctga 2280
catatctttc tctgaaattc catgcacatg tttagggaca tcaaaggagc aaacatattg 2340
gtagatccaa atggtcgggt caaggttgca gactttggca tggcaaaaca tgtaagctta 2400
gagatgataa ctgtccagtt tccattatat tttcatttag ctgaaaatta gattcttttt 2460
tttttttgac aaaaaaaaat tagattctaa tgcttattga aattataaat cataggtttt 2520
ttttctaaaa aatctcaata tatcgatgtt tctggcttct tgatagcaat tgggcgattt 2580
aaaatgttaa aagaaaatgt gaagatggaa ccctattttt aagtatggaa agagaaacca 2640
gaataataca aaattgtgat ggtgtatgat ttaccttgta aaccacgatt tatgctgtcc 2700
aggacgtgca tgatttgtcc catttgtact tacaattaca tgattcacag ataacagggc 2760
aatactgtcc attgtcattc aaaggaagtc cttattggat ggctcccgag gttctacttt 2820
ttcttttttg ggttcagttc tttagatttt aattgcattt tagttttgtc actcattatc 2880
ttttcttttt gatgattcag gttataaaga attccaagga atgcagcctt ggtgtggata 2940
tatggagtct tggatgcaca gttttggaaa tggctacaac gaagcctcct tggtctcagt 3000
atgaaggggt tagtaattca tgtataatac atgaggtttt atatttacaa agacctggtg 3060
atatttttgt attataaaca tgtcaacttt taaattaatt cttcttactg cttggtgtat 3120
ttacctgtta ctataacagg ttgctgccat gttcaaaatt ggtaacagca aggagctccc 3180
aacaattcca gatcatctct caaatgaagg aaaagatttt gttagaaaat gtctacaacg 3240
taatccacgt gatcgccctt cagcaagtga attattagaa cacccttttg taaaaggtgc 3300
tgcacctttg gaaagaccta ttatggttcc cgaagcttca gaccctatta ctggaattac 3360
acatggaaca aaagctctgg tgcgtaacct atttcctact ccaatcgttg gaaactcact 3420
tcagttgtgt ttgtgtggtt gttcatgtta ccattcgatg gtactttgtc tttcatttta 3480
tgcgaatatc aaactgtcgc attgccttat ctacactata gaacaaaata ggggacagtt 3540
tgctctcttc atgaaaatgt catgtgaact tctgttcttg ttttcacatt tctcatggtt 3600
gagtaattta agtggcgtgt aaattttata ggctattcac gctcttagta atacataaat 3660
gtaaagattt tgatccgtca agtccaaaat atcgagtcaa ttgcgtgatc tatattttca 3720
ttcctttttc tgtgtaagtt attaaggaaa ttgttctttg tatgaggcag catgaatgat 3780
gcataaggga aaaacataga tgatagctca atacatagta ggttgaggga tagtgagata 3840
ttggttggaa agcgataaag ttctagggtt tttaaattgg tttccctttt catcagatct 3900
ggctagaatt ttatgtcaaa agtatacaca tggagttcag ttaggatgga gacttttgtt 3960
atttcaattc agtgtaattt attttaaaag taatataaat cttgtttcgt gtttattagg 4020
atgaagtatc ttccaaatat gaggtcacaa caagtttttc ttgcatcaat gagttagctt 4080
tgtactactc aatctgtatt ctcttgtttt atttttataa taattgcatc aatttcaggg 4140
tattggacaa ggaaggaatc tgtctgcctt ggattcagat aaactcttgg ttcattcttc 4200
tagggttttg aaaaataatc ctcatgaaag gtatctctct agctcactca cccacgcgca 4260
cacacacaca cacacacaaa aatgcatgca aggctgttga cattgccaat ggtaatctga 4320
aattatttca tcaatttcca gtgaaatcca tattcaaagg aatatatctt gcccagtctc 4380
tcccattgga agcccacttt tgaggtcaag atcaccacag cagaggagtg ggagactgtc 4440
tccttctcct atatctagcc ctcggaccgc ttctggtgca tccacgcctc tcactggcgg 4500
cagtggtgcc attccattca gtaaccactt aaaacaatca gtttactttc aagagtgtct 4560
cggaagcatg ccgaaatccc caaatggtgc ctacattaac ggctcttctc accacgattc 4620
aaatattgac atttttcaaa gaatgcaagc cgggtctcac attaaatcag aactagtctc 4680
cggtgataat gatgctcttg gcaagcagtt cgtaaggtct cctcatgcag aaccgtatga 4740
ctttcagtcg gtcttagctg atcgtgttgg ccggcagctt ctgggggatc atgttaagat 4800
taacccgtca tttgatccaa gtcccagctc atcgatgctc aaccggacaa atggtttatg 4860
a 4861
<210> 35
<211> 6271
<212> DNA
<213> 百脉根(Lotus japonicus)
<400> 35
atgccttcat ggtgggggaa accatcgtca aaagaaacca agaagaaggc aagtaaggaa 60
agttttattg acacactaca acgaaaattt aaaagcccat ccgaaggtaa actaagcagt 120
agatctggag gatccggtag acgttgcaat gacacaattt cagaaaaggg ggctcagtct 180
cctcctgaat cgagatctcc ttcaccttcc aaagttgcaa ggtgtcaaag ttttgctgaa 240
aggcctcgtg ctcaaccatt gcctcttcct ggcctgcatc catctaatat aagccgagca 300
gattctgaaa ttagcatatc atctaaaata agacaggaaa agagctccaa acaatcactg 360
tttccgccgt taccaagacc tgcatgcatg cgtggtcgac taaaccctgg agatttggat 420
agtgatttga tcactgcttc agtctctagt gagagctctg ctgatagtga tgaaccattg 480
gactcccgca atcgcagtcc tctggcgact gactccgaga ctgggactag aactgctgca 540
ggcagtcctt caaggtatac tcatgaccat tcaggataat gtgcttccag attgcatatt 600
tcgttaaatt acttgtatgc atgggttatt tatgattact tgcaacttat tttttacttt 660
actcatctcg gatgttggca ttttaatgaa gcctaatgct caaggatcaa tcagctgctg 720
ttgcccaagt aaattcaagg gaagccaaaa agccggcaaa cattctgggt aatatgtctt 780
ctacttcacc aaaacggagg cctttaagca accatgttcc aaatctgcag attcctcctc 840
atggtgcctt ttgtagcgct cctgacagtt ccaggtcaag tccatcaaga agtccgttga 900
gagcatttgg caccgaacaa gtattgaact ctgcgttttg ggctggaaag ccatatacag 960
aggtcaattt tgttgggtct gggcagtgct caagtccagg ttctggacac aattctgggc 1020
acaattcaat gggaggggac atgtcaggac agttattttg gcaaccaagc aggggtagcc 1080
ctgaatattc tcctgtacct agtcccagaa tgactagccc tggtccaagc tctagaattc 1140
agagtggagc tgttacacct attcatccca gagctggggt aacatctgct gaatcacaaa 1200
taggacgggt tgatgatggg aaaaaacaga gccaccgttt gccccttcct cctttagctg 1260
ttaccaattc catgcctttc tctcattcaa attctgcagc aacatctcca tccatgccga 1320
gaagtccagg aagagcagat aatccaatga gccctggttc acgttggaaa aaaggaaagc 1380
tacttggcag aggcacattt gggcatgtct atgttggctt caataagtat gtacttagga 1440
acaaaatttg ttgattttat tatgaactaa ttcttggaaa aaaacattca tgtgaagttg 1500
taaacatcat cttaacaaaa ttgcttgatg caaatgtttg tctcgtttag ggaaagtggt 1560
gaaatgtgtg caatgaaaga ggtgactctg ttttctgatg atgccaagtc taaggaaagt 1620
gctaagcaat taatgcaggt aaattgattt atttttagac ccaggaaaat ttcatggaat 1680
atctatatgt tttaaatgta atgtaccttt gaaactctaa ctcctagatt cttgcaggaa 1740
atttccttat taagccgttt acagcatccg aatattgtgc agtattacgg ttctgaaaca 1800
gtaagctctt catttcctgt ttcattcaca ttcggataat tatgttagca tctgaatatt 1860
ggtccatttc atatatatgc atgattgctg gtggtttacc tttttatcta acagcattat 1920
attagaaaca tgtttgcatt gtttgttttt catctactta tagagcttct gactgttccc 1980
ttaaacaggt agatgacaag ctttacatat acctggagta tgtagctgga ggttccatat 2040
ataaacttct tcaagaatat gggcagtttg gtgagctagc tattcgtagt tatactcaac 2100
aaattttgtc agggcttgct tatttgcatg ctaaaaacac tatccacagg tgaactaagt 2160
cgcatatatt tttagattat aaattttact tctgccacat aatgattaat tttctacact 2220
tactgatata tctgacaaaa tttgcatttt tcttgtaaag ggacatcaaa ggagcaaatt 2280
tactagtaga tactaatggg cgggtcaagt tggcagactt tggcatggcc aaacatgtaa 2340
gcttggagat taaaactatc cattttataa ttgacattta agctgataga gaatatgagg 2400
ttctgatgct tagagaaatt atgaatcaat tacaaaatat catggattta ctaattcaag 2460
ggagatgcta ttgccaattc tctggggttt attcatgtta gtagtttttt ttcctgcccg 2520
aaatcttacc cattaaacgc gcatgtcttt aacttaaaaa aatatgacat aacccaaaaa 2580
tctttcacaa agactttaga ttcatttggt tatagtgtta accagatgtt gggttcctgg 2640
ttaattgcca tattatattg catctggttt atttatttat ttttattctt aatgctttca 2700
ataatcacaa ggttgcataa ttcttgcttt ttacccaact tgcatatttt gcagataaca 2760
gggcaatcat gtccattatc attcaaagga agcccttatt ggatggcacc tgaggttgta 2820
ttatttttct attagcatct gttctttata gattaaacta cgtctcagtt ttgtgacttc 2880
acatctctgc tcctaattaa tcaggttata aaaaactcta gtgggtgcaa tcttgcggta 2940
gatatatgga gtcttggatg cactgttttg gaaatggcta caactaagcc tccttggagt 3000
caatatgaag gggtttgtaa aatatcttgg aactttaaat tatttctttt gtcctaatat 3060
atatgtactg ttttttcatt tttataaagt actgatgata attctagtat taacttggct 3120
tggcttaatg tagtattgca tttgtgggtt aacataacag gttgctgcca tgttcaagat 3180
tggtaatagc aaggaactcc caacaatacc agatcatctc tctaatgaag gaaaggattt 3240
tgttcggaag tgcctacaac gtaatcctca acagcgtcct tcagctagtg atttgttgaa 3300
tcaccctttt gtaaaatgtg ctgcaccttt agaaagacct attctccttc ctgaagcttc 3360
agaccccgta tctgagattg cacatggatc aaaagctctg gtacagaaac tattcccaac 3420
ataattcata taaaaataag tcttcttctg ttgtatctcc atattgtcat tattttagcc 3480
agggatgggt tgtttgtctt gcattgcatg ccatggttta atgtcaaata ttgtttctcc 3540
cttattcctc tatgcatggt aggaataaaa cataaagcca tctggtcctt tacatgaaaa 3600
tattacataa ttttctgcag ttcactcttt tgaatccctc ctgagtgatg aatcagtttt 3660
gtagttatgt atattgtata gacaattcat tctcttggaa atgcaaagat gaccaatttt 3720
aatctaccag agaatatttg ggctaatgaa atatctttct tctatccgtt gtccatatat 3780
gttgccatag aagttaaaca tttttctgag gcagcataag ggtatagaag ggaaaggcag 3840
gtacagcagc ttgagaaaga tttagtgttg aaggataggg tgatagaagc aggattggaa 3900
ctgaatgaag gttctaggtt taggacaccc tcagttctgg aatccaatag attacataga 3960
aattccttga ataattgtga atgaaaaaat attatatcta aattatttta cacatgggcc 4020
cgcgttcatg tagaaatagt acaactttat cagctcttct aggacttttc taatccattt 4080
atataataat ttaccatcat aatcaccttt aaattatctt aagtaagtta taatagtata 4140
caaagaacag ctattaaaat tactaaaggc ctattcggtt tgttttttca cttcctgttt 4200
tcaatttaca ttatcagttt taattatata atagtcacct tgttttctct ttgtttcatg 4260
ttttcaaagt tttgtactgg aaacagtgaa aataatgaaa cagggaacaa attgaaaata 4320
acgtggtggg tgttataatt aaaactgaac agaaataaaa ataaaaccaa ataggcccta 4380
aaatattcag aagtcaaaac atcactctaa attgcagcag ggcaaaatag tggacatttg 4440
tagtcgaata atgttttgtt gacactcgaa acacatctac acctagtgag atacaagaag 4500
aaaagagaga ggattaatgg ataatgtgat aggaaaagaa aagagatagg aagagaaggt 4560
gagtggaaat gaaatgtaag agaaagtgaa gtatgaatga aatactctac cttttcaaag 4620
tattacctga taagggactt tttagtttga acagaaaacc ttcatggtga ttttaaattt 4680
cagactcatt attatgatag agactggttc agaagataga cattaacaga aagaaaataa 4740
ggaaatttta ctgaatatcc ttccccaaat ctcgatcaat ggatatccag ggtcaaaaat 4800
ggcaagaatg atgtacaatg ttatcttcct gatgaattca aactagttcc ccctacccaa 4860
aactgactga ctaactaact cctgtcctgt accttcgctg cctctattta taactgctta 4920
cctaattaac tattgacaat ttacgaggcc tgctctcaac ttacaaagcg taactaactg 4980
gggctggcca ttttgatccc ttaggccctt attcttgtag accttcctaa ttggaggttg 5040
catgctaaca tcctgcagaa actatgatat gtttgtttac atattagtag agaaggattc 5100
ttcttcttgc tgaaacttta agttttttac taatgactcg tgttattgaa tcccttcgta 5160
ttagaggatg aagacacagc tatacagaca caatgagaaa aactgtatta aagtataact 5220
aaggagtttt tcattacctt gggtaaaaca aacacttgga agttcagagg ggaaagcaaa 5280
acaaagataa gggcttttct gtcatcataa gaattgtggg gttgaatatt ttttatcagc 5340
aaaataaagt tagtttgtta cctctccatg tggaagttcc aaagtttctc tagattaggt 5400
attcaattga actatcttcc tattataaga tactagaaat ttttgcttgc atcaatgaag 5460
tgatctttga acttgttttc cctttgtggt ttctcctgta ttctcttgtt atttttgtgg 5520
taatctcatt aatcattgat aaattttcag ggcattggac aaggacggaa tctttctatg 5580
ttggattcag atagactttc tcttcattct aggtttttga aaaataatcc tcatgcaagg 5640
tctctctctc tctctctctg tctgactctc tctctctctc gcacacacag tcacaccatg 5700
cacacacaag ggtgatgaca ttgcccatgg taatctaaca ttattttaat tattgatctc 5760
cagtgagctc catattccaa ggaatatatc ctgccctgtc tctcccatag gaagcccact 5820
tttgagacca agatcaccac agcatatgag cggaagaatg tctccgtctc ctatatctag 5880
ccctcggact gcttctggtg catccacacc tctcagtggt ggtagtggtg ccattccatt 5940
tagtaataac ttagtttact ttcaagaggg tcttggaagc ttgccaaagt ccccaaacgg 6000
catctacatt agtggtcctg ctcatcctga ctcaaatgtt gacattttcc gaggaatgca 6060
gaagacttct cacatttcat cggaactggt tcccagtgaa aatgatgctc tgggcaagca 6120
gtttaccagg cttcctcaag acaagtcata tgatgttcaa tcagtcttgg ctgatcgtgt 6180
ttgccggcag ctgctcgggg ataatgtgaa gattaaccca tctattgatc taagacccaa 6240
caccaatctg ctcagccgag caaatggttt a 6271
<210> 36
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
gactttattg gtgataccag gtcg 24
<210> 37
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
ggtcaaccac acgggtactg taa 23
<210> 38
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
gtgcaatgat ggaggttact c 21
<210> 39
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
cggttgggaa acagcagatg 20
<210> 40
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
cggaccttca acaagatcgc 20
<210> 41
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
aaggacgttt ctggcactgt 20
<210> 42
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
ttgggtggct atctgtttgg a 21
<210> 43
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
cccttgccct ctgaagtatg t 21
<210> 44
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
acaggatgca gatcatagtg c 21
<210> 45
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
tgggcaacaa gatccaaact g 21
<210> 46
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
gagagtgtga aaggaggcca 20
<210> 47
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
acctccacaa gccagtcaac 20
<210> 48
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
ctgaccaagc cggatcaact 20
<210> 49
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
ggctgagcag atggttcaga 20
<210> 50
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
ggtggtgtgt ttcgggtagt 20
<210> 51
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
caccgtcact ctcagcatca 20
<210> 52
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
tgatgcggaa ggcactatgg 20
<210> 53
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
gcattgccca catgaagctc 20
<210> 54
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
tgcgtagggc atttggaaca 20
<210> 55
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
ctccacccaa tacagcccag 20
<210> 56
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
ccacatggct gttcatggtg 20
<210> 57
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
tggtgtggag ccttcattaa ct 22
<210> 58
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
tgcagggcca gaaagagatg 20
<210> 59
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
tcacgattac cggtcccatt c 21
<210> 60
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
gggtgaggaa ctgtcgtctg 20
<210> 61
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
ggatccattg gtcttgcttg c 21
<210> 62
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
tcgacggtat cgataagctt ccaggttaat ataggctcga ctg 43
<210> 63
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
ggcggccgct ctagaactag atttgtacta gataaattga g 41
<210> 64
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
ggcggccgct ctagaactag cttcagaaac agggaaactt 40
<210> 65
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
cgcggatccg gaaggaatct gtctgccttg 30
<210> 66
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
aaaaaagctt tggggatttc ggcatgcttc 30
<210> 67
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
aaaactgcag tggggatttc ggcatgcttc 30
<210> 68
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
gagagaacgc gtgggactct tgaccatggt a 31

Claims (10)

1.一种突变的蒺藜苜蓿CLL1基因,其特征在于,与Medtr1g100250相比,所述突变的蒺藜苜蓿CLL1基因第一内含子缺失,所述缺失的第一内含子序列如SEQ ID NO.1所示。
2.根据权利要求1所述突变的蒺藜苜蓿CLL1基因,其特征在于,所述突变的蒺藜苜蓿CLL1基因的核苷酸序列如SEQ ID NO.2所示,其编码的CLL1蛋白序列如SEQ ID NO.3所示。
3.根据权利要求2所述CLL1蛋白在调控植物侧生器官伸长中的应用,其特征在于,所述调控为正向调控。
4.一种蒺藜苜蓿cll1突变体,其特征在于,与野生型相比,所述cll1突变体中包含权利要求1所述突变的蒺藜苜蓿CLL1基因,其核苷酸序列如SEQ ID NO.2所示。
5.一种改良植物的方法,其特征在于,所述方法包括调低CLL1基因表达,例如去除所述CLL1基因的第一内含子或者用RNA干扰技术降低CLL1基因表达。
6.根据权利要求5的方法,其特征在于,用于RNA干扰的片段如SEQ ID NO.4所示。
7.一种融合表达载体,所述融合表达载体包括待表达基因序列和插入替换的所述CLL1基因的第一内含子。
8.根据权利要求7的融合表达载体,所述待表达基因序列如SEQ ID NO.5所示;所述CLL1基因的第一内含子序列如SEQ ID NO.1所示。
9.权利要求2所述的CLL1蛋白的豆科直系同源蛋白,其特征在于,所述蛋白的氨基酸序列如SEQ ID NO.6-20所示,所述豆科植物包括:鹰嘴豆(Cicer arietinum)、豌豆(Pisumsativum)、大豆(Glycine max)、菜豆(Phaseolus vulgaris)、赤豆(Vigna angularis)、落花生(Arachis hypogaea)、蔓花生(Arachis duranensis)、绿豆(Vigna radiata)车轴草(Trifolium pratense)、紫花苜蓿(Medicago sativa)和百脉根(lotus japonicus)。
10.权利要求7所述CLL1蛋白的豆科直系同源蛋白的编码基因,其特征在于,所述编码基因的核苷酸序列如SEQ ID NO.21-35所示。
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022257697A1 (zh) * 2021-06-10 2022-12-15 中国科学院西双版纳热带植物园 调控植物半矮化株型和叶比值的cll1基因及其豆科的直系同源基因的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074617A2 (en) * 1999-07-29 2001-02-07 Helix Research Institute Primers for synthesising full-length cDNA and their use
CN110819635A (zh) * 2019-11-04 2020-02-21 山东大学 豆科植物han同源基因在调控豆科植物根瘤数目中的应用
CN112063626A (zh) * 2019-06-10 2020-12-11 中国农业大学 玉米基因ZmRAVL1和功能位点及其用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109486839B (zh) * 2018-11-09 2021-05-11 山东农业大学 一种拟南芥mapkkk激酶在育种中的应用
CN110042113B (zh) * 2019-05-17 2021-05-14 中国科学院东北地理与农业生态研究所 水稻粒型正调控基因OsMAPKKK70、其编码蛋白及其应用
CN114085852B (zh) * 2021-06-10 2024-02-13 中国科学院西双版纳热带植物园 调控植物半矮化株型和叶比值的cll1基因及其豆科的直系同源基因的应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074617A2 (en) * 1999-07-29 2001-02-07 Helix Research Institute Primers for synthesising full-length cDNA and their use
CN112063626A (zh) * 2019-06-10 2020-12-11 中国农业大学 玉米基因ZmRAVL1和功能位点及其用途
CN110819635A (zh) * 2019-11-04 2020-02-21 山东大学 豆科植物han同源基因在调控豆科植物根瘤数目中的应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AES62473.1: "ATP-binding protein [Medicago truncatula]", GENBANK *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022257697A1 (zh) * 2021-06-10 2022-12-15 中国科学院西双版纳热带植物园 调控植物半矮化株型和叶比值的cll1基因及其豆科的直系同源基因的应用

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