CN109757277B - Method for rapidly recovering root system activity of magnolia denudata seedlings - Google Patents
Method for rapidly recovering root system activity of magnolia denudata seedlings Download PDFInfo
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- CN109757277B CN109757277B CN201910144402.1A CN201910144402A CN109757277B CN 109757277 B CN109757277 B CN 109757277B CN 201910144402 A CN201910144402 A CN 201910144402A CN 109757277 B CN109757277 B CN 109757277B
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- 240000005819 Magnolia denudata Species 0.000 title claims abstract description 43
- 235000016094 Magnolia denudata Nutrition 0.000 title claims abstract description 43
- 230000000694 effects Effects 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000002689 soil Substances 0.000 claims abstract description 50
- 239000003337 fertilizer Substances 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000005556 hormone Substances 0.000 claims abstract description 18
- 229940088597 hormone Drugs 0.000 claims abstract description 18
- 239000011573 trace mineral Substances 0.000 claims abstract description 18
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 11
- 238000011084 recovery Methods 0.000 claims abstract description 7
- 239000011248 coating agent Substances 0.000 claims abstract description 5
- 238000000576 coating method Methods 0.000 claims abstract description 5
- 230000010412 perfusion Effects 0.000 claims abstract description 5
- 238000004804 winding Methods 0.000 claims abstract description 5
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 16
- 150000001875 compounds Chemical class 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 9
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical group C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 8
- 239000003617 indole-3-acetic acid Substances 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 6
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 6
- -1 alkyl glycoside Chemical class 0.000 claims description 6
- 229930182470 glycoside Natural products 0.000 claims description 6
- 229940083466 soybean lecithin Drugs 0.000 claims description 6
- 241000589157 Rhizobiales Species 0.000 claims description 5
- 239000004114 Ammonium polyphosphate Substances 0.000 claims description 4
- 229920002752 Konjac Polymers 0.000 claims description 4
- 235000019826 ammonium polyphosphate Nutrition 0.000 claims description 4
- 229920001276 ammonium polyphosphate Polymers 0.000 claims description 4
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 235000013312 flour Nutrition 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- 239000000022 bacteriostatic agent Substances 0.000 claims description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 3
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 3
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 239000002121 nanofiber Substances 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 239000004408 titanium dioxide Substances 0.000 claims description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 229920002125 Sokalan® Polymers 0.000 claims description 2
- 125000000129 anionic group Chemical group 0.000 claims description 2
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 2
- 239000004584 polyacrylic acid Substances 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 2
- 230000009467 reduction Effects 0.000 abstract description 12
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 7
- 241000735598 Galbulimima belgraveana Species 0.000 description 3
- 241000235527 Rhizopus Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 239000002131 composite material Substances 0.000 description 2
- YJTKZCDBKVTVBY-UHFFFAOYSA-N 1,3-Diphenylbenzene Chemical group C1=CC=CC=C1C1=CC=CC(C=2C=CC=CC=2)=C1 YJTKZCDBKVTVBY-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 235000013532 brandy Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a magnolia denudata seedling with quick root activityThe method for quick recovery comprises the following steps of coating a soil ball pipe film: winding the transplanted magnolia denudata seedling soil balls on the upper layer and the lower layer of the soil balls in a row respectively by using PVC pipes, and then wrapping the upper layer and the lower layer of the PVC pipes and wrapping the soil balls by using a film material; nutrient solution perfusion: injecting nutrient solution consisting of hormone, trace elements and fertilizer into the PVC pipe from the upper opening, and infiltrating a film wrapping the soil ball along the upper PVC pipe; repeating the steps every 3 to 4 weeks to finish the rapid recovery of the root system activity of the magnolia denudata seedlings. The large magnolia denudata tree planted in early spring by adopting the technical scheme of the invention has the advantages that the root activity is recovered to the normal state from the weak state within three months; or from a weaker state to a strong state (the root system reduction strength is 15-20)μgV (g.h), mean 17.5μg/(g.h)）。
Description
Technical Field
The invention relates to a method for quickly recovering the root activity of a magnolia denudata seedling, in particular to a method for quickly recovering the root activity of a large-size magnolia denudata seedling after transplanting.
Background
The brandy root is a fleshy root, is not resistant to water and moisture, is sensitive to saline-alkali soil, and is slow to recover due to the reduction of root activity caused by the lack of soil nutrition, unsmooth ventilation and drainage and the like after the large-size nursery stock is transplanted.
During the transplanting process of the magnolia denudata, the root system is damaged due to root breaking during seedling lifting; the conditions of the planting points on the land do not conform to the ecological habits of the large magnolia denudata trees, for example, the pH value of soil is slightly alkaline, the soil is sticky and heavy to cause unsmooth ventilation, the terrain is slightly low or the underground water level is slightly high to cause unsmooth drainage, the nutrition deficiency of the soil and the like cause the root system of the magnolia denudata trees to be underdeveloped, the vitality of the root system after transplantation is frustrated, and the reduction strength of the root system is often 0-5μgV (g.h), mean 2.5μgV (g.h). Generally, the root system of the magnolia denudata can be recovered after one year or more, and even the magnolia denudata is in a weak or weak state for a long time, so that the large tree grows badly for a long time.
In the transplanting process, the big seedlings are dug to damage the root system, and possible damage is caused in the loading, unloading and transporting processes; the difference between the conditions of the planting field and the original habitat, especially the great difference between the conditions of the planting field and the ecological requirements of the magnolia denudata, is a main reason for the difficulty in transplanting.
Conventional cultivation techniques such as raising the terrain to relatively reduce the groundwater level, applying organic media to improve the soil structure and the like are methods for indirectly recovering and enhancing the root activity by increasing the permeability and avoiding ponding, have certain effect in the long term, but have unobvious effect on rapidly recovering the root activity.
Disclosure of Invention
Aiming at the straight root system oblique rooting type of the white magnolia, the method for quickly restoring the root system vitality by wrapping the soil ball with the white magnolia pipe film is researched and developed by aiming at quickly restoring the root system vitality of the transplanted large-size white magnolia seedling (the chest diameter is more than 20 cm). The method comprises the following specific steps:
(1) and (3) coating the soil ball pipe film: winding the transplanted magnolia denudata seedling soil balls on the upper layer and the lower layer of the soil balls in a row respectively by using PVC pipes, and then wrapping the upper layer and the lower layer of the PVC pipes and wrapping the soil balls by using a film material;
(2) nutrient solution perfusion: injecting nutrient solution consisting of hormone, trace elements and fertilizer into the PVC pipe from the upper opening, and infiltrating a film wrapping the soil ball along the upper PVC pipe;
(3) repeating the step (2) once every 3-4 weeks to finish the rapid recovery of the root system activity of the magnolia denudata seedlings.
In the step (1), the pipe orifice of the upper layer pipe obliquely leaks out of the soil surface upwards, and the lower layer pipe converges into the drainage ditch from the bottom of the tree pit at a gradient of 3-5 degrees. In the precipitation and the later period, the PVC pipe can remove the excessive water permeating into the pipe. After the internal seepage water is discharged, the inner space of the pipe has the function of ventilation.
The membrane material is any one of carboxymethylated plant fiber or carboxymethylated nanofiber grafted carboxymethyl cellulose, sodium polyacrylate, anionic polyacrylamide and polyvinyl alcohol containing polyacrylic acid chain segments, and the molecular weight of the grafted material is 10000-120000. The film material has the functions of water absorption and water locking, so that liquid is infiltrated and slowly released, and the film can be naturally degraded in soil within 3-6 months. The nutrient solution flowing through the PVC pipe can uniformly permeate into the film-shaped or net-shaped material, and is uniformly and slowly released from the side surface of the soil ball and absorbed by soil and root systems on the side surface of the soil ball.
The grafting material is also added with a titanium dioxide bacteriostatic agent with the mass fraction of 0.001-0.01%.
The mass ratio of the hormone to the trace elements to the fertilizer is (1-5): (10-20): 10000.
the fertilizer is N, P, K-containing compound fertilizer, and the compound fertilizer is also added with ammonium polyphosphate modified konjaku flour composite material, and the addition amount of the composite material is 0.1-0.8% of the mass of the fertilizer.
The hormone is naphthylacetic acid and indoleacetic acid; the hormone also comprises collecting rhizosphere soil during the digging process of the big magnolia denudata tree, dissolving the soil in clear water, standing at normal temperature for 2-3h, filtering, and using the filtrate as rhizobacteria liquid (mainly umbrella-shaped mildew) symbiotic with the root system of the magnolia denudata treeUmbelopsisBacteria), naphthylacetic acid and indoleacetic acid account for 0.1-0.8% of the mass of the rhizopus liquid.
The microelements are ferrous sulfate and copper sulfate, and alkyl glycoside or soybean lecithin is also added into the microelements, wherein the addition amount of the alkyl glycoside or the soybean lecithin is 0.01-0.1% of the mass of the microelements.
Through the functions of increasing soil nutrition and hormone, ventilating and draining water, the root system activity of the magnolia denudata transplanted seedlings is quickly recovered in the first three months, the seedling revival period is shortened, and a good foundation is laid for subsequent healthy growth.
In the technical scheme of the invention, the activity of the root system is measured by adopting a triphenyltetrazolium chloride (TTC) method. TTC is dissolved in water to form a colorless solution, but after reduction, red and insoluble triphenyl methylhydrazone (TTF) is generated, and the reduction strength of the TTC is used for expressing the activity of the root system.
TTC reduction Strength: (μg/(g, h)) = amount of TTC reduction (TTC reduction amount: (g, h) =μg) /[ root weight (g) × time (h)]
The activity of the root system is frustrated to a normal level (the reduction strength of the root system is 10 to 15)μgV (g.h), mean 12.5μg/(g.h)), recovery is required. The method specifically comprises the following steps: weak activity and root system reduction strength of 5-10μgV (g.h), mean 7.5μgV (g.h); weak activity and root system reduction strength of 0-5μgV (g.h), mean 2.5μg/(g. h)。
Through the measures of the patent: in the big magnolia denudata tree planted in early spring, the root activity is recovered to the normal state from the weak state within three months; or from a weaker state to a strong state (the root system reduction strength is 15-20)μgV (g.h), mean 17.5μg/(g. h)）。
Detailed Description
A method for rapidly recovering the root activity of magnolia denudata seedlings comprises the following steps:
(1) and (3) coating the soil ball pipe film: winding the transplanted magnolia denudata seedling soil balls on the upper layer and the lower layer of the soil balls in a row respectively by using PVC pipes, wrapping the PVC pipes on the upper layer and the lower layer by using a membrane material, wrapping the PVC pipes on the upper layer and the lower layer and wrapping the soil balls, enabling pipe orifices of the upper layer of pipes to obliquely leak upwards to the soil surface, and enabling the lower layer of pipes to converge into drainage ditches from the bottoms of the tree pits at a gradient of 5;
(2) nutrient solution perfusion: injecting nutrient solution consisting of hormone, trace elements and fertilizer into the PVC pipe from the upper opening, and infiltrating a film wrapping the soil ball along the upper PVC pipe; the membrane material is carboxymethyl cellulose grafted by carboxymethylated plant fiber, and the molecular weight is 12000. The mass ratio of the hormone to the trace elements to the fertilizer is 5: 14: 10000, wherein the fertilizer is a compound fertilizer containing N, P, K, the compound fertilizer is also added with a compound material of ammonium polyphosphate modified konjaku flour, and the addition amount of the compound material is 0.3 percent of the mass of the fertilizer. The hormone is naphthylacetic acid and indoleacetic acid; the hormone also comprises collecting rhizosphere soil during the digging process of the big magnolia denudata tree, dissolving the soil in clear water, standing at normal temperature for 2-3h, filtering, and using the filtrate as rhizobacteria liquid (mainly umbrella-shaped mildew) symbiotic with the root system of the magnolia denudata treeUmbelopsisBacteria), naphthylacetic acid and indoleacetic acid account for 0.5 percent of the mass of the rhizopus liquid. The trace elements are ferrous sulfate and copper sulfate, and alkyl glycoside is also added in the trace elements, wherein the addition amount of the alkyl glycoside is 0.06% of the mass of the trace elements.
(3) Repeating the step (2) once every 3-4 weeks to finish the rapid recovery of the root system activity of the magnolia denudata seedlings.
By adopting the technical scheme, the root activity, the root length and the root number after 3 months have obvious effects, and the method specifically comprises the following steps:
TABLE 1 root vigor and growth status of magnolia denudata planted in early spring
Note: the counted area of the root number and the root length is 900cm2The surface of the soil ball; root activity unit:μg/(g. h)。
as can be seen from the above table 1, compared with CK, by adopting the patent technology, the root system activity of the large magnolia denudata tree can be quickly recovered and remarkably improved within 3 months after the large magnolia denudata tree is planted in early spring, and the number and the length of new roots are also obviously increased. The big magnolia denudata tree which does not adopt the technology of the patent has obviously lower root activity, root length and root quantity than the big magnolia denudata tree which is processed by the technology under the condition that the nursery stock and the planting condition are the same. CK root activity cannot be recovered in a long time (half a year or even 1 year or more).
Example 2
A method for rapidly recovering the root activity of magnolia denudata seedlings comprises the following steps:
(1) and (3) coating the soil ball pipe film: winding the transplanted magnolia denudata seedling soil balls on the upper layer and the lower layer of the soil balls in a row respectively by using PVC pipes, wrapping the PVC pipes on the upper layer and the lower layer by using a membrane material, wrapping the PVC pipes on the upper layer and the lower layer and wrapping the soil balls, enabling pipe orifices of the upper layer of pipes to obliquely leak upwards to the soil surface, and enabling the lower layer of pipes to converge into drainage ditches from the bottoms of the tree pits at a gradient of 5;
(2) nutrient solution perfusion: injecting nutrient solution consisting of hormone, trace elements and fertilizer into the PVC pipe from the upper opening, and infiltrating a film wrapping the soil ball along the upper PVC pipe; the membrane material be carboxymethylated's nanofiber grafted anion polyacrylamide, the post-grafting molecular weight is 80000, the grafting material in still add the titanium dioxide bacteriostatic agent that the mass fraction is 0.001%. The mass of the hormone, the trace elements and the fertilizer is 1: 12: 10000, the fertilizer contains N, P, KThe compound fertilizer is also added with a compound material of ammonium polyphosphate modified konjaku flour, and the addition amount of the compound material is 0.6 percent of the mass of the fertilizer. The hormone is naphthylacetic acid and indoleacetic acid; the hormone also comprises collecting rhizosphere soil during the digging process of the big magnolia denudata tree, dissolving the soil in clear water, standing at normal temperature for 2-3h, filtering, and using the filtrate as rhizobacteria liquid (mainly umbrella-shaped mildew) symbiotic with the root system of the magnolia denudata treeUmbelopsisBacteria), naphthylacetic acid and indoleacetic acid account for 0.6 percent of the mass of the rhizopus liquid. The trace elements are ferrous sulfate and copper sulfate, and soybean lecithin is also added into the trace elements, wherein the addition amount of the soybean lecithin is 0.03 percent of the mass of the trace elements.
(3) Repeating the step (2) once every 3-4 weeks to finish the rapid recovery of the root system activity of the magnolia denudata seedlings.
TABLE 1 root vigor and growth status of magnolia denudata planted in early spring
Note: the counted area of the root number and the root length is 900cm2The surface of the soil ball; root activity unit:μg/(g. h)。
Claims (4)
1. a method for rapidly recovering the root activity of magnolia denudata seedlings is characterized by comprising the following steps:
(1) and (3) coating the soil ball pipe film: winding the transplanted magnolia denudata seedling soil balls on the upper layer and the lower layer of the soil balls in a row respectively by using PVC pipes, then wrapping the PVC pipes on the upper layer and the lower layer by using a membrane material, wherein the membrane material is any one of carboxymethylated plant fibers or carboxymethylated nano-fibers grafted carboxymethyl cellulose, sodium polyacrylate, anionic polyacrylamide and polyvinyl alcohol containing polyacrylic acid chain segments, and the molecular weight after grafting is 10000-120000;
(2) nutrient solution perfusion: injecting nutrient solution consisting of hormone, trace elements and fertilizer into a PVC pipe from an upper opening, and infiltrating a film wrapping a soil ball along the upper PVC pipe, wherein the trace elements are ferrous sulfate and copper sulfate, and the trace elements are also added with alkyl glycoside or soybean lecithin, the addition amount of the alkyl glycoside or the soybean lecithin is 0.01-0.1% of the mass of the trace elements, the fertilizer is a compound fertilizer containing N, P, K, the compound fertilizer is also added with a compound material of ammonium polyphosphate modified konjaku flour, the addition amount of the compound material is 0.1-0.8% of the mass of the fertilizer, and the hormone is naphthylacetic acid and indoleacetic acid; the hormone also comprises the steps of collecting rhizosphere soil in the process of digging up the magnolia denudata trees, dissolving the soil in clear water, standing for 2-3h at normal temperature, filtering, taking filtrate as rhizobacteria liquid symbiotic with the magnolia denudata roots, wherein naphthylacetic acid and indoleacetic acid account for 0.1-0.8% of the mass of the rhizobacteria liquid;
(3) repeating the step (2) once every 3-4 weeks to finish the rapid recovery of the root system activity of the magnolia denudata seedlings.
2. The method for rapidly recovering the root activity of the magnolia denudata seedlings according to claim 1, wherein in the step (1), the soil surface leaks from the mouth of the upper-layer pipe obliquely upwards, and the lower-layer pipe converges into a drainage ditch from the bottom of the pit at a gradient of 3-5 degrees.
3. The method of claim 1, wherein a titanium dioxide bacteriostatic agent with a mass fraction of 0.001-0.01% is further added to the grafting material.
4. The method for rapidly recovering the root activity of the magnolia denudata seedlings according to claim 1, wherein the mass ratio of the hormone to the trace elements to the fertilizer is (1-5): (10-20): 10000.
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| CN108738997A (en) * | 2018-06-08 | 2018-11-06 | 濮阳市园林绿化处 | A kind of Nursery Stock inoculation method |
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