CN109757248B - Method for inhibiting flowering of mikania micrantha by using combined exogenous hormone - Google Patents
Method for inhibiting flowering of mikania micrantha by using combined exogenous hormone Download PDFInfo
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- CN109757248B CN109757248B CN201910225730.4A CN201910225730A CN109757248B CN 109757248 B CN109757248 B CN 109757248B CN 201910225730 A CN201910225730 A CN 201910225730A CN 109757248 B CN109757248 B CN 109757248B
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- Prior art keywords
- mikania micrantha
- regulator
- combined
- gibberellin
- hormone
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- 241001440840 Mikania micrantha Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000005556 hormone Substances 0.000 title claims abstract description 24
- 229940088597 hormone Drugs 0.000 title claims abstract description 24
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 17
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 title claims abstract description 15
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 13
- 239000003448 gibberellin Substances 0.000 claims abstract description 13
- 239000003617 indole-3-acetic acid Substances 0.000 claims abstract description 13
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 4
- 239000011259 mixed solution Substances 0.000 claims abstract description 4
- 229940057847 polyethylene glycol 600 Drugs 0.000 claims abstract description 4
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 4
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 239000000575 pesticide Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 6
- 230000033228 biological regulation Effects 0.000 abstract description 3
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000228293 Persicaria perfoliata Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000036531 allelopathy Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 229940125697 hormonal agent Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of agricultural production, and particularly relates to a method for inhibiting flowering of mikania micrantha by using combined exogenous hormone, which takes preparation of a regulator with the concentration of 1L being 10ppm as an example and comprises the following steps: s1, weighing 10mg of indoleacetic acid and 10mg of gibberellin, and dissolving the indoleacetic acid and the gibberellin in 3mL of ethanol; s2, transferring the S1 solution into a 1L volumetric flask; s3, adding water into the volumetric flask to a constant volume of 1L to obtain 10ppm of regulator; s4, transferring the regulator solution prepared in the S3 into a 2L big beaker; s5, adding 1mL of polyethylene glycol 600 and 1mL of Tween 20 nonionic surfactant into a 2L big beaker; s6, stirring the mixed solution in the S5 at room temperature for 30 minutes to obtain the combined hormone regulator; the exogenous hormone regulation and control method adopted by the invention is a safe and effective method for inhibiting the mikania micrantha from blooming and fructifying, the spread of the mikania micrantha is controlled from seed propagation, and the method for continuously controlling the mikania micrantha is simple and convenient to operate, low in cost, free of pollution and the like.
Description
Technical Field
The invention belongs to the technical field of agricultural production, and particularly relates to a method for inhibiting mikania micrantha from blooming by using combined exogenous hormone.
Background
Mikania micrantha is an exotic invasive organism, which is mainly propagated by seeds and grows strongly, nearly 20cm each day 1, and is called "mile a minute weed" (one mile of weeds). The world natural protection alliance (IUCN) published 2001 as one of the most harmful 100 alien invasive species in the world; in 2004, mikania micrantha is listed in a national forestry quarantine pest list for the first time; in 2013, 1 and 9 days, mikania micrantha is determined as the only national forestry quarantine harmful plant by the bulletin of the national forestry bureau (No. 4 in 2013), and so far, mikania micrantha is also the only national forestry major quarantine harmful plant. Due to the strong allelopathy, the fast growth speed and the strong shading effect, the growth of crops and forest vegetations in an invasive land is inhibited, even the vegetation is disappeared after the crops and the forest vegetations are wound, climbed and covered by the mikania micrantha, the whole high and large trees die, the yield of the crops and the economic forest is reduced, and the forest biodiversity disappears in partial areas.
At present, methods for preventing and treating mikania micrantha comprise artificial mechanical removal, chemical prevention and treatment, biological prevention and treatment and substitution control. Among them, biological control and chemical control are the two most important methods. The methods have good effects in severe emergence areas of farmland mikania micrantha, but the pesticide for preventing and removing the mikania micrantha in the forest land is not widely applied to preventing and removing the mikania micrantha in the forest land due to the influences of the factors such as inconvenient use of the herbicide, high cost, environmental pollution and the like.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a method for inhibiting the flowering of mikania micrantha by using combined exogenous hormone, which has the characteristics of convenience in use and low cost.
In order to achieve the purpose, the invention provides the following technical scheme: a method for inhibiting flowering of mikania micrantha by using combined exogenous hormones, for example, a regulator with the concentration of 10ppm is prepared by 1L, and comprises the following steps:
s1, weighing 10mg of indoleacetic acid and 10mg of gibberellin, and dissolving the indoleacetic acid and the gibberellin in 3mL of ethanol;
s2, transferring the S1 solution into a 1L volumetric flask;
s3, adding water into the volumetric flask to a constant volume of 1L to obtain 10ppm of regulator;
s4, transferring the regulator solution prepared in the S3 into a 2L big beaker;
s5, adding 1mL of polyethylene glycol 600 and 1mL of Tween 20 nonionic surfactant into a 2L big beaker;
s6, stirring the mixed solution in the S5 at room temperature for 30 minutes to obtain the combined hormone regulator;
s7, spraying the pesticide 1 week before the blooming of mikania micrantha in 10 months, and spraying the pesticide 1 time in the full bloom stage in 11 middle-late months.
As a preferable technical scheme of the method for inhibiting the flowering of the mikania micrantha by using the combined exogenous hormone, the nonionic surfactant aims to increase the stability of the regulator solution.
As a preferable technical scheme of the method for inhibiting the flowering of mikania micrantha by using the combined exogenous hormone, the mass ratio of the indoleacetic acid to the gibberellin is 1: 1.
as a preferable technical scheme of the method for inhibiting the flowering of the mikania micrantha by using the combined exogenous hormone, the indoleacetic acid is used as a hormone reagent for stimulating the growth of plants.
As a preferred technical scheme of the method for inhibiting the flowering of mikania micrantha by using the combined exogenous hormone, the gibberellin is a widely-existing phytohormone.
As a preferable technical scheme of the method for inhibiting the flowering of mikania micrantha by using the combined exogenous hormone, in the S7, the dosage of the medicament is 100 kg/mu.
Compared with the prior art, the invention has the beneficial effects that: the exogenous hormone regulation and control method adopted by the invention is a safe and effective method for inhibiting the mikania micrantha from blooming and fructifying, the spread of the mikania micrantha is controlled from seed propagation, and the method for continuously controlling the mikania micrantha is simple and convenient to operate, low in cost, free of pollution and the like.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a graph of the inflorescence of normal mikania micrantha of the present invention;
fig. 3 is a graph of the inflorescence of mikania micrantha after combined hormone treatment according to the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1, the present invention provides a technical solution: a method for inhibiting flowering of mikania micrantha by using combined exogenous hormones, for example, a regulator with the concentration of 10ppm is prepared by 1L, and comprises the following steps:
s1, weighing 10mg of indoleacetic acid and 10mg of gibberellin, and dissolving the indoleacetic acid and the gibberellin in 3mL of ethanol;
s2, transferring the S1 solution into a 1L volumetric flask;
s3, adding water into the volumetric flask to a constant volume of 1L to obtain 10ppm of regulator;
s4, transferring the regulator solution prepared in the S3 into a 2L big beaker;
s5, adding 1mL of polyethylene glycol 600 and 1mL of Tween 20 nonionic surfactant into a 2L big beaker;
s6, stirring the mixed solution in the S5 at room temperature for 30 minutes to obtain the combined hormone regulator;
s7, spraying the pesticide 1 week before the blooming of mikania micrantha in 10 months, and spraying the pesticide 1 time in the full bloom stage in 11 middle-late months.
In particular, the purpose of the nonionic surfactant is to increase the stability of the conditioner solution.
Specifically, the mass ratio of the indoleacetic acid to the gibberellin is 1: 1.
in particular, the indoleacetic acid is useful as a hormonal agent for stimulating plant growth.
In particular, the gibberellins are a ubiquitous plant hormone.
Specifically, in S7, the dosage of the medicament is 100 kg/mu.
Example 2
Referring to fig. 2-3, flowers of mikania micrantha are head-shaped inflorescences which are arranged in a complex chamber inflorescence shape at the branch end, the inflorescences are thin, the head-shaped inflorescences at the top are opened first and gradually and sequentially downwards, the head-shaped inflorescences contain 4-5 flowers (fig. 2), after the combined hormone regulator treatment, the mikania micrantha flower buds cannot be normally differentiated, the number of the inflorescences and the flowers is reduced (fig. 3), the seed development is inhibited, and the flowering inhibition rate of the mikania micrantha reaches 72.3%.
The inhibition rate (%) - (control group flower number-experimental group flower number)/(control group flower number) × 100%.
Control group: water-sprayed mikania micrantha.
Experimental groups: mikania micrantha treated with spray regulator.
The inhibition rate (%) of the hormone regulator with different concentrations and proportions on the flowering of mikania micrantha.
From the viewpoint of the production cost and the inhibitory effect of the hormone-regulating agent, 10ppm of the combined regulating agent is used at an optimum concentration.
The invention has the beneficial effects that: the exogenous hormone regulation and control method adopted by the invention is a safe and effective method for inhibiting the mikania micrantha from blooming and fructifying, the spread of the mikania micrantha is controlled from seed propagation, and the method for continuously controlling the mikania micrantha is simple and convenient to operate, low in cost, free of pollution and the like.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. The method for inhibiting the flowering of mikania micrantha by using the combined exogenous hormone is characterized by comprising the following steps of preparing 1L of regulator with the concentration of 10ppm as an example: s1, weighing 10mg of indoleacetic acid and 10mg of gibberellin, and dissolving the indoleacetic acid and the gibberellin in 3mL of ethanol; s2, transferring the S1 solution into a 1L volumetric flask; s3, adding water into the volumetric flask to a constant volume of 1L to obtain 10ppm of regulator; s4, transferring the regulator solution prepared in the S3 into a 2L big beaker; s5, adding 1mL of polyethylene glycol 600 and 1mL of Tween 20 nonionic surfactant into a 2L big beaker; s6, stirring the mixed solution in the S5 at room temperature for 30 minutes to obtain the combined hormone regulator; s7, spraying the pesticide 1 week before the flowering of mikania micrantha in 10 months, and spraying the pesticide 1 time in the full-bloom stage in 11 middle-late-month; the mass ratio of the indoleacetic acid to the gibberellin is 1: 1.
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| CN201910225730.4A CN109757248B (en) | 2019-03-25 | 2019-03-25 | Method for inhibiting flowering of mikania micrantha by using combined exogenous hormone |
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| CN201910225730.4A CN109757248B (en) | 2019-03-25 | 2019-03-25 | Method for inhibiting flowering of mikania micrantha by using combined exogenous hormone |
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| CN109757248B true CN109757248B (en) | 2021-06-01 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147483A (en) * | 2007-11-09 | 2008-03-26 | 北京市园林科学研究所 | Inhibitor for plant flying-batt and its use |
| CN101715785A (en) * | 2009-11-26 | 2010-06-02 | 李翠兰 | Special growth inhibitor for Eupatorium adenophorum |
| CN103120163A (en) * | 2013-03-26 | 2013-05-29 | 广州金水动物保健品有限公司 | Disinfector containing BCDMH (Bromochloro Hydantoin) and preparation method thereof |
| CN106172481A (en) * | 2016-07-14 | 2016-12-07 | 南京农业大学 | A kind of plant seed developmental inhibitors for controlling weeds and application thereof |
| CN108522541A (en) * | 2018-06-07 | 2018-09-14 | 四川省兰月科技有限公司 | Liniment for promoting grape maturity hyperchromic |
| CN108617672A (en) * | 2018-06-15 | 2018-10-09 | 广东中绿园林集团有限公司 | It is a kind of prevention Mikania micrantha composition and its application |
-
2019
- 2019-03-25 CN CN201910225730.4A patent/CN109757248B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147483A (en) * | 2007-11-09 | 2008-03-26 | 北京市园林科学研究所 | Inhibitor for plant flying-batt and its use |
| CN101715785A (en) * | 2009-11-26 | 2010-06-02 | 李翠兰 | Special growth inhibitor for Eupatorium adenophorum |
| CN103120163A (en) * | 2013-03-26 | 2013-05-29 | 广州金水动物保健品有限公司 | Disinfector containing BCDMH (Bromochloro Hydantoin) and preparation method thereof |
| CN106172481A (en) * | 2016-07-14 | 2016-12-07 | 南京农业大学 | A kind of plant seed developmental inhibitors for controlling weeds and application thereof |
| CN108522541A (en) * | 2018-06-07 | 2018-09-14 | 四川省兰月科技有限公司 | Liniment for promoting grape maturity hyperchromic |
| CN108617672A (en) * | 2018-06-15 | 2018-10-09 | 广东中绿园林集团有限公司 | It is a kind of prevention Mikania micrantha composition and its application |
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