CN113068699A - Plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application - Google Patents

Plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application Download PDF

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Publication number
CN113068699A
CN113068699A CN202010049828.1A CN202010049828A CN113068699A CN 113068699 A CN113068699 A CN 113068699A CN 202010049828 A CN202010049828 A CN 202010049828A CN 113068699 A CN113068699 A CN 113068699A
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China
Prior art keywords
plant growth
growth regulator
brassinolide
cytokinin
regulator composition
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CN202010049828.1A
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Chinese (zh)
Inventor
李志江
楚娟娟
林建海
许秀宝
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Jiangxi Xinbang Biochemical Co ltd
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Jiangxi Xinbang Biochemical Co ltd
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Priority to CN202010049828.1A priority Critical patent/CN113068699A/en
Publication of CN113068699A publication Critical patent/CN113068699A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.001-1 parts of brassinolide, 0.001-0.1 parts of chlorin iron and 0.0001-10 parts of cytokinin (one of benzylaminopurine, adenine, furfuryl aminopurine or hydroxyadenine). The method can promote the flower and fruit retention of the citrus, increase the fruit setting rate after the first and second physiological fruit dropping, prevent flower and fruit dropping, improve the fruit agronomic characters (transverse and longitudinal diameters, single fruit weight and the like), improve the citrus quality (soluble solid content, VC content and sugar content), increase the yield greatly and obviously improve the citrus yield; does not cause phytotoxicity to crops, is highly safe to the crops, and is environment-friendly and safe.

Description

Plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application
Technical Field
The invention relates to the technical field of agricultural growth regulators, in particular to a plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof.
Background
The plant growth regulator is extracted and synthesized artificially based on the structure, function and action principle of plant hormone, and can change the synthesis, transportation, metabolism and action of hormone in plant body to regulate plant growth and physiological function. Auxins, gibberellins, ethylene, cytokinins, abscisic acid, brassinolide, and the like have been found. The plant growth regulator can promote or inhibit the growth and development of plants at a lower concentration. Plant growth regulators have been used in crops, fruit trees and vegetables for a wide range of applications.
At present, the single use of many plant growth regulators cannot achieve the ideal effect, and the disadvantages of the single use of the plant growth regulators are as follows: the biological activity is single, the growth regulating effect is relatively low, the use amount (or use concentration) is large, and the growth regulating effect can be achieved only in a certain growth period of crops generally. Therefore, it is necessary to properly and rationally select and mix different plant growth regulators in order to overcome or avoid the side effects, thereby achieving the desired application effects, i.e., synergy, addition or induction, and avoiding antagonism.
Brassinolide, a novel green environment-friendly plant growth regulator, is treated by soaking seeds and spraying stems and leaves of brassinolide with proper concentration. Can promote the growth of vegetables, melons, fruits and other crops, improve the quality, increase the yield, and ensure bright color and thicker leaves. The tea leaf picking time can be advanced, and the melon and fruit can have higher sugar content, larger individual, higher yield and better storage stability. Is harmless to human and livestock, and has safe and effective dosage when used normally. The natural brassin can be widely applied to various economic crops, can increase the yield by 5-10% and can reach 30% higher, obviously improve the quality, increase the sugar content and the weight of fruits and increase the gorgeous crops. Meanwhile, the drought resistance and the cold resistance of crops can be improved, and the symptoms of plant diseases and insect pests, phytotoxicity, fertilizer damage and freezing damage of the crops can be relieved.
The chlorin iron (III) chelate is prepared by chelating chlorin iron (III) chelate with pyropheophorbide, purpurin and chlorin as main ligands and different acid radicals or hydroxide radicals as axial ligands (X) and transition metal ferric ions. The plant growth regulator is safe and nontoxic and is not easy to accumulate residues; the activity is strong, and the effect is good; can improve the chlorophyll content of plants, promote rooting, improve germination rate, enhance stress resistance and increase yield.
Benzylaminopurine is high-activity cytokinin, has high activity, can increase chlorophyll, improve photosynthetic efficiency, effectively promote cell division and differentiation of plants, promote flower bud differentiation, accelerate growth and development, strengthen plants, strengthen fruits and expand fruits, and can improve yield.
Adenine is one of cytokinins. The plant growth regulator is prepared by pure biological fermentation, is mainly secreted and conducted to other parts by a root tip, stimulates cell division, promotes chlorophyll formation, and prevents premature senility and fruit shedding. Promoting photosynthesis and protein synthesis, and promoting flower bud differentiation and formation. And has good control effect on tomato, cucumber and tobacco virus diseases. The application range is grain, cotton, oil, vegetables, melons and fruits, medicinal materials, tobacco and the like.
Hydroxyalkenyladenine is one of cytokinins. Can stimulate plant cell division, promote chlorophyll formation, accelerate plant metabolism and protein synthesis, thereby achieving the purposes of rapidly increasing organism, promoting crops to be mature and high yield, and improving the disease resistance, ageing resistance and cold resistance of plants.
Furfuryl amino purine, one of cytokinins. The 1 st cytokinin discovered by human beings is a substance capable of promoting the division and differentiation of plant cells, is widely present in seaweed and most of plant bodies, belongs to an endogenous (natural) plant growth regulator which is produced by the plant bodies and has the function of regulating the growth of plants, and mainly has the function of promoting the division, differentiation and growth of the cells; inducing the callus to grow buds and removing the apical dominance; breaking dormancy of lateral buds and promoting seed germination; also has the functions of delaying the aging of leaves and cut flowers in vitro; inducing bud differentiation and development and increasing stomatal aperture; regulating transport of nutrients; promoting fructification and the like.
Citrus (Citrus reticulata Blanco) is a generic name of Citrus, Citrus reticulata, Citrus aurantium, Citrus grandis, and Poncirus trifoliata. China is one of the important native areas of citrus, the citrus resources are rich, the excellent varieties are various, the cultivation history is more than 4000 years, and the citrus becomes a precious fruit for human beings through long-term cultivation and selection. The flowering period of the citrus is generally 4-5 months, and the fruit period is 10-12 months. In recent years, due to the factors of various environmental problems such as global warming, haze and the like, the citrus has a lot of obstacles and troubles in the planting process, so that the effective measures adopted to protect flowers and fruits of the citrus become links which need to be broken by continuous efforts of agricultural workers.
Flower and fruit retention is an important means for improving the fruit setting rate of the citrus in the stable yield load capacity range. The purpose of flower and fruit retention is to improve the fruit setting rate, and the fruit setting rate is an important factor influencing the yield of the citrus. Therefore, the flower and fruit dropping is reduced by implementing the flower and fruit retention measures, the fruit setting rate is improved, and particularly the fruit setting rate is improved in the years with less flowers, so that the method is a key link for obtaining high yield of the citrus. The natural fruit setting rate of the citrus is generally low, and the increase of the weight of a single fruit is limited by the inheritance of varieties. Through continuous practice, the fact proves that artificial reasonable measures are continuously taken according to the natural growth rule of the oranges and the change factors of the oranges, so that the flowers and the fruits can be effectively preserved, and the loss is reduced.
At present, the conventional treatment technology for flower and fruit retention in actual production comprises the steps of improving the storage nutrition level of trees, properly controlling the vegetative growth, ensuring good pollination conditions, improving the environmental conditions, applying growth regulators and trace elements and the like. However, the existing efficient and cost-effective ternary plant growth regulator for flower and fruit retention is not common, so that the formula of the novel ternary plant growth regulator for flower and fruit retention of citrus becomes one of the hot development fields.
The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and it is not necessarily prior art to the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.001-1 parts of brassinolide, 0.001-0.1 parts of chlorin iron and 0.0001-10 parts of cytokinin.
Preferably, the composition consists of the following components in parts by weight: 0.01 part of brassinolide, 0.005 part of chlorin iron and 5 parts of cytokinin.
Preferably, the brassinolide is selected from any one of 28-homobrassinolide, 24-epibrassinolide, 25-methyl brassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide or 14-hydroxy brassinosteroid.
Preferably, the cytokinin is selected from any one of benzylaminopurine, adenine, furfurylaminopurine, or hydroxyadenine.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture and an auxiliary agent allowed by pesticides into any one of missible oil, microemulsion, aqueous emulsion, suspending agent, oil suspending agent, dry suspending agent, wettable powder or water dispersible granules.
The invention also aims to protect the plant growth regulator composition and the application thereof in citrus cultivation. Compared with the prior art, the invention has the following advantages and positive effects:
(1) the invention can adjust endogenous hormone of citrus, promote the transverse and longitudinal growth of citrus plants and fruits, promote the protoplasm flow of citrus pulp cells, improve the cell activity, improve the quality of citrus and improve the yield of citrus.
(2) The invention can promote the pollination of the citrus, protect flowers and fruits, improve the fruit setting rate after the first and second physiological fruit dropping, prevent flower dropping and fruit dropping and reduce the abnormal fruit rate; high safety to oranges and environment protection.
Detailed Description
The following is a detailed description of specific embodiments of the invention.
Example 1
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.01 part of 28-high brassinolide, 0.005 part of chlorin iron and 5 parts of benzylaminopurine.
The preparation method of the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into wettable powder with 2.5 percent of active ingredient mass percentage with auxiliary agents allowed in pesticides according to a conventional preparation method.
Example 2
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 24-epibrassinolide 0.03 parts, chlorin iron 0.075 parts, and adenine 0.0075 parts
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into an oil suspending agent with 5% of active ingredients in percentage by mass according to a conventional preparation method by using auxiliary agents allowed in pesticides.
Example 3
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.05 part of 25-methyl brassinolide, 0.05 part of chlorin iron and 0.4 part of furfuryl aminopurine.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into a suspending agent with 7.5 percent of active ingredients in percentage by mass according to a conventional preparation method by using auxiliary agents allowed in pesticides.
Example 4
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: propionyl brassinolide 0.075 part, chlorin iron 0.025 part and hydroxyl adenine 0.001 part.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into water dispersible granules with 10% of active ingredients in percentage by mass according to a conventional preparation method with auxiliary agents allowed in pesticides.
Example 5
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 28-epihomobrassinolide 0.1 part, dihydroporphin iron 0.01 part and adenine 0.0001 part.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing missible oil with 12.5 percent of active ingredients by mass percent with auxiliary agents allowed in pesticides according to a conventional preparation method.
Example 6
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.01 part of mixotrophic brassinolide, 0.005 part of chlorin iron and 2.5 parts of benzylaminopurine.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into the microemulsion with the mass percent of active ingredients of 15% by using an auxiliary agent allowed in pesticides according to a conventional preparation method.
Example 7
A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are disclosed, wherein the plant growth regulator composition comprises the following components in parts by weight: 0.04 part of 14-hydroxyl brassinosteroid, 0.001 part of chlorin iron and 10 parts of benzylaminopurine.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into an aqueous emulsion with 20% of active ingredients by mass percent according to a conventional preparation method with an auxiliary agent allowed in pesticides.
Test of field drug effect
A place: hainan province Qiongzhong countyGreen orange citrus orchard
Time: 2 months in 2019 to 10 months in 2019
Crop: citrus fruit
Soil: yellow loam
Test protocol: the plant growth regulator compositions of the present invention of examples 1 to 7 and comparative examples 1 to 3 were selected. Comparative examples are 28-homobrassinolide, chlorin iron and benzylaminopurine, respectively.
The plant growth regulator composition and application of the invention are designed into 7 treatments, and the treatment-1 (adopting the plant growth regulator composition and application of the invention in the embodiment 1), the treatment-2 (adopting the plant growth regulator composition and application of the invention in the embodiment 2), the treatment-3 (adopting the plant growth regulator composition and application of the invention in the embodiment 3), the treatment-4 (adopting the plant growth regulator composition and application of the invention in the embodiment 4), the treatment-5 (adopting the plant growth regulator composition and application of the invention in the embodiment 5), the treatment-6 (adopting the plant growth regulator composition and application of the invention in the embodiment 6) and the treatment-7 (adopting the plant growth regulator composition and application of the invention in the embodiment 7) are numbered in sequence;
comparative example 1: 0.01% 28-high brassin formulation alone, comparative example 2: 0.02% chlorin iron formulation alone, comparative example 3: 5% benzylaminopurine formulation alone. The test treatments are respectively sprayed once in 3 and 12 days in 2018, 4 and 1 days in 2018 and 5 and 1 days in 2018. Comparative examples 1 to 3 were sprayed for the same period of time, and the test results are shown in table 1 below.
TABLE 1 comparison of the plant growth regulator composition of the present invention with the comparative example
Figure BSA0000200596630000061
The results show that compared with the highest fruit setting rate of comparative examples 1-3, the fruit setting rate after the first and second physiological fruit drops after the plant growth regulator composition and the application (treatment 1-7) are sprayed is obviously higher, the fruit setting rate after the first physiological fruit drop is respectively increased by 1.2-3.82%, and the fruit setting rate after the second physiological fruit drop is respectively increased by 1.56-2.50%.
Compared with the maximum yield increase of comparative examples 1-3, the application of the plant growth regulator composition and the application (treatment 1-7) increase the yield of citrus to a certain extent, and increase the yield by 6.67-14.56% respectively. Among them, the yield increase effect of example 1 was the most significant, and the yield per plant was increased by 7.43kg and 23.94% as compared with the control. Meanwhile, the single fruit weight of the citrus is obviously increased by spraying the plant growth regulator composition (treatment 1-7), and the single fruit weight of the citrus in examples 1-7 is increased by 16.88-23.91% compared with that of the control. Therefore, the plant growth regulator composition and the application thereof provided by the invention promote the transverse and longitudinal diameter growth of citrus fruits, and improve the single fruit weight and the single plant yield of citrus, thereby improving the yield of citrus.
The surface and section of the citrus fruit in each test group were observed, and the soluble solids content, VC content and sugar content of the fruit pulp were measured, and the results are shown in Table 2 below.
TABLE 2 Effect of the plant growth regulator compositions of the present invention and the comparative examples on Citrus quality
Figure BSA0000200596630000071
The results show that compared with comparative examples 1-3, the soluble solid content, Vc content and sugar content of the citrus fruit applied by applying the plant growth regulator composition (treatment 1-7) are higher than those of the comparative examples, and the citrus fruit quality is improved to a certain extent by applying the plant growth regulator composition and the application thereof.
The above embodiments are merely preferred embodiments of the present invention, and any simple modification, modification and substitution changes made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims (5)

1. A plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application thereof are characterized in that the plant growth regulator composition comprises the following components in parts by weight: 0.001-1 parts of brassinolide, 0.001-0.1 parts of chlorin iron and 0.0001-10 parts of cytokinin (one of benzylaminopurine, adenine, furfuryl aminopurine or hydroxyadenine).
2. The plant growth regulator composition comprising brassinolide, chlorin iron and cytokinin as claimed in claim 1, wherein said brassinolide is selected from any one of 28-homobrassinolide, 24-epibrassinolide, 25-methyl brassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide or 14-hydroxy brassinosteroid.
3. The plant growth regulator composition comprising brassinolide, chlorin iron and cytokinin as claimed in claim 1 and use thereof, wherein said cytokinin is selected from any one of benzylaminopurine, enoadenine, furfurylaminopurine or hydroxyenoadenine.
4. The plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and the application of the plant growth regulator composition are characterized in that the raw materials in the weight ratio are uniformly mixed and are prepared into any one of missible oil, microemulsion, emulsion in water, suspending agent, oil suspending agent, dry suspending agent, wettable powder or water dispersible granules with auxiliary agents allowed in pesticides.
5. The plant growth regulator composition comprising brassinolide, chlorin iron and cytokinin according to claim 1 and use thereof in citrus planting.
CN202010049828.1A 2020-01-05 2020-01-05 Plant growth regulator composition containing brassinolide, chlorin iron and cytokinin and application Withdrawn CN113068699A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106007934A (en) * 2016-05-17 2016-10-12 中国农业科学院茶叶研究所 Foliar fertilizer capable or promoting early germination of tea trees and application of foliar fertilizer
CN114885954A (en) * 2022-06-01 2022-08-12 张掖市林业科学研究院 Plant growth regulator composition for promoting seed germination

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106007934A (en) * 2016-05-17 2016-10-12 中国农业科学院茶叶研究所 Foliar fertilizer capable or promoting early germination of tea trees and application of foliar fertilizer
CN106007934B (en) * 2016-05-17 2023-11-07 中国农业科学院茶叶研究所 Foliar fertilizer for promoting early germination of tea trees and application thereof
CN114885954A (en) * 2022-06-01 2022-08-12 张掖市林业科学研究院 Plant growth regulator composition for promoting seed germination

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Application publication date: 20210706