CN106172481B - It is a kind of for the vegetable seeds developmental inhibitors of controlling weeds and its application - Google Patents
It is a kind of for the vegetable seeds developmental inhibitors of controlling weeds and its application Download PDFInfo
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- CN106172481B CN106172481B CN201610556973.2A CN201610556973A CN106172481B CN 106172481 B CN106172481 B CN 106172481B CN 201610556973 A CN201610556973 A CN 201610556973A CN 106172481 B CN106172481 B CN 106172481B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
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Abstract
The present invention discloses a kind of for the vegetable seeds developmental inhibitors of controlling weeds and its application, which includes plant growth regulator and water；The main component of the plant growth regulator is glyphosate.The vegetable seeds developmental inhibitors further include at least one of nutritional ingredient and sticker, preferably further include nutritional ingredient and sticker.Vegetable seeds developmental inhibitors of the invention inhibit bud differentiation and Seed Development, prevent seed and generate and spread sprawling, reach the technology for administering weeds.The vegetable seeds developmental inhibitors that the present invention filters out are low using concentration, environmentally safe.Currently, the report still without related research Eupatorium adenophorum seed inhibitor both at home and abroad, the research of this respect enters to invade to prevent and kill off and will provide one of new way to the solution grass.
Description
Technical field
The present invention be it is a kind of for the vegetable seeds developmental inhibitors of controlling weeds and its application, belong to plant protection subject
Control of weeds technical field.
Background technique
Since the 1940s, first 2.4-D of selective herbicide was born, the use of chemical herbicide is to peasant
Many benefits are brought with agricultural production, and with the progress of science and technology, herbicide new varieties are continually developed, in pesticide
Specific gravity is increasing.But chemical herbicide, which is brought, also brings serious negative effect such as Environmental security, weeds while interests
Drug resistance, chemical residual etc..Therefore seek low dosage, high-drug-effect herbicide become the field emphasis.Glyphosate is one
Kind of wide spectrum goes out natural disposition, nonselective herbicide, and extremely low to the toxicity of environment, degradable, residual is few.Therefore, Developing
Transgenosis glyphosate resistant crops, glyphosate herbicidal are widely used in the crop fields, have become most important herbicide type, make
Dosage has accounted for 20% or more of entire herbicide market.Currently, in the removing action for the exotic invasive weed that China carries out,
Also glyphosate is widely used to be prevented and kill off.But, using glyphosate management of weeds, routine uses concentration 0.1% or more,
Perennial Alien Weeds are prevented and kill off, then at concentrations up to 0.8% or more.Cause in this way it is huge prevent and kill off cost, preventing and kill off Alien Weeds
While, other plants in all habitats have also been killed, bio-diversity is greatly reduced.In addition, also having aggravated environment
Pollution.
Eupatorium adenophorum (Eupatorium adenophorum Spreng.), Solidago Canadensis, Horseweed Herb, artemisiifolia,
False stinkweed, bidentis, annual fleabane herb, Radix Conyzae sumatrensis, spring bitter fleabane, Ageratum conyzoides, guayule rubber, fragrant eupatorium herb, Mikania micrantha etc. are pernicious
Exotic invasive weed.These weeds are local places at China's field planting initial stage, still, as its invasion is spread, are spread to
Vast region seriously threatens the bio-diversity generation on invasion ground, brings about great losses to ecological environment and economic development.
The reason of Alien Weeds invasive first is that its with strong fertility, mainly hip number is big, seed is small and light but with hat
Hair, easily transmitted, diffusion, therefore its Seed Development is prevented, Alien Weeds can be effectively reduced and further spread and endanger.
Conventional weed control methods (manually, mechanically and chemically preventing and kill off) can play the Alien Weeds for preventing and kill off a small range
Certain positive effect, but for the area overflowed, artificial and mechanical anti-division is time-consuming and laborious；Largely use herbicide
Although whole strain can be killed, but also the useful plant of surrounding can be killed and bring serious environmental pollution；Dirt caused by biological insect control
Though dye is low, current control not enough, can only control its growth, without can control the big of its seed to a certain extent
Amount is propagated and diffusion.The efficient seed inhibitor of low dosage is developed, prevents Alien Weeds from blossoming and bearing fruit, not only can effectively prevent and kill off
The weeds are to bleeding around, breed, spread, and environmentally safe property is high.Currently, being there is no both at home and abroad in relation to passing through weed seed
The technology of inhibitor control weeds also more lacks, and the exploitation of the inhibitor will be that a comparison is safe, have long-range meaning
The prevention and control new way of justice.
Summary of the invention
The purpose of the present invention is to provide a kind of vegetable seeds developmental inhibitors.
Another object of the present invention is to provide above-mentioned vegetable seeds developmental inhibitors in the application administered in weeds.
The purpose of the present invention is achieved through the following technical solutions:
A kind of vegetable seeds developmental inhibitors, the vegetable seeds developmental inhibitors include plant growth regulator and
Water；The main component of the plant growth regulator is glyphosate.
As a kind of optimal technical scheme, the vegetable seeds developmental inhibitors further include in nutritional ingredient and sticker
At least one preferably further includes nutritional ingredient and sticker.
The weight ratio of the plant growth regulator and the nutritional ingredient is 490:0.5~300, preferably 490:1
~250, further preferably 490:10~250 are still more preferably 490:30~200；
The weight ratio of the plant growth regulator and the binder is 490:1~150, preferably 490:1~
100, further preferably 490:2~100 are still more preferably 490:5~50.
Concentration of the nutritional ingredient in the plant growth and development inhibitor is 0.05%~30% (100ml plant
The nutritional ingredient containing 0.05~30g in seed development inhibitor) but not limited to this, preferably 3%~22%；The sticker exists
Concentration in the plant growth and development inhibitor be 0.01%~15% (in 100ml vegetable seeds developmental inhibitors containing 0.01~
15g sticker) but not limited to this, preferably 0.02%~10%, it is still more preferably 0.5%~5%.
The sticker is selected from peach gum, xanthan gum, gelatin, Arabic gum, dextrin, starch, agar, carboxymethyl cellulose
At least one of；The nutritional ingredient is selected from least one of sucrose, urea and ammonium hydrogen carbonate；It is preferred: described
Nutritional ingredient is urea and sucrose, or is ammonium hydrogen carbonate and sucrose；It is further preferred that the weight of the urea and sucrose
Than for 1:1~1:5, the weight ratio of the ammonium hydrogen carbonate and sucrose is 1:1~1:5；The sticker is peach gum.
The glyphosate is glyphosate and its esters；Preferably, the glyphosate salt is glyphosate isopropyl amine
At least one of salt, ammonium glyphosate, glyphosate sylvite, glyphosate sodium salt and glyphosate dimethylamine salt.
It further include ethephon (CEPHA),2-(chloroethyl) phosphonic acid in the plant growth regulator；Preferably, the weight ratio of the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
≥3:1；Further preferably, the weight ratio of the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid is 50:1~4:1；Still more preferably, the grass is sweet
The weight ratio of phosphine and ethephon (CEPHA),2-(chloroethyl) phosphonic acid is 20:1~5:1；Still more preferably, the weight ratio of the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid be 20:1~
10:1。
Above-mentioned vegetable seeds developmental inhibitors can using plant growth regulator as principle active component with nutritional ingredient and
At least one of sticker is auxiliary agent, along with other auxiliary agents allowed on pesticide are configured to any one allowed on pesticide
Dosage form.
Application of the above-mentioned vegetable seeds developmental inhibitors in controlling weeds.
Taking the vegetable seeds developmental inhibitors to be configured to plant growth regulator is 0.25%~0.004% concentration
(plant growth regulator containing 0.005~0.1g in 100ml vegetable seeds developmental inhibitors) sprays, and the use of the time is weeds bud
Idiophase puts peak period to the flowers are in blossom.
The weeds be farmland and Alien Weeds Eupatorium adenophorum, Solidago Canadensis, Horseweed Herb, artemisiifolia, false stinkweed,
Bidentis, annual fleabane herb, Radix Conyzae sumatrensis, spring bitter fleabane, Ageratum conyzoides, guayule rubber, fragrant eupatorium herb, Mikania micrantha.
Heretofore described concentration " % " is the grams of certain solute included in 100ml preparation.
Glyphosate is preparing vegetable seeds developmental inhibitors or is inhibiting the developmental application of vegetable seeds.The plant species
Sub- developmental inhibitors include plant growth regulator and water, and the main component of the plant growth regulator is glyphosate；It is preferred that
, the vegetable seeds developmental inhibitors further include at least one of nutritional ingredient and sticker；It is further preferred that described
Plant growth regulator in further include ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
Although in the present invention using glyphosate as herbicide, the function of killing weeds is famous, at low dose
The lower technology as seed inhibitor of amount processing is to pass through a large amount of multiple experiment sievings, when blooming for Eupatorium adenophorum
Each period (flower bud differentiation period, megaspore and microspore cytological development phase, peak period of blooming application), the solid best suppression of screening control Eupatorium adenophorum
Formulation concentrations and most sensitive application period thoroughly block under the premise of taking into account environmental protection and human health and cause purple stem pool
The new function of the seed source of orchid sprawling, diffusion.Glyphosate, which passes through, inhibits enolpyruvyl shikimene phosphate synthase (EPSPS),
Lead to aromatic amino acid biosynthesis block, so that the synthesis of protein is unsmooth, inhibit the growth and development process of plant, until killing
Plant.The target that the art of this patent is related to is to need to inhibit the reproductive growth process of plant, finally fruit development is made to be obstructed, but not
It can influence the normal nutrient growth process of plant.Therefore, the key of technology is using during vegetation growth of plant and reproductive growth
To glyphosate sensitive sex differernce, realize the purpose for only influencing and inhibiting reproductive growth process.So the key of the art of this patent
Property technological progress be to solve to guarantee the medicament residence time as long as possible on branch under low concentration processing, and be conducive to
Sorbefacient measure synergistic effect, so that giving full play to for low dosage medicament drug effect is realized, to significantly reduce medicament
Use concentration.Its principle is the initial stage that spraying time is in that trees just start recovery growth, and number metabolism is weaker, to what is used
Medicament absorption is slower, and spring rainwater is more in addition, easily causes effective component elution to fall, sticker can reduce the hair of this process
It is raw, and nutriment may advantageously facilitate absorption, the synergistic effect of the two significantly reduces the usage amount of medicament.First to inhibitor
It is screened using concentration, when discovery concentration reaches 0.1%-0.005%, that is, can inhibit the normal of the adventitious plants such as Eupatorium adenophorum
Development, so that the tapetal cell of anther abnormal behaviour is occurred, i.e., disintegrate in advance, cause pollen mother cell nonnutritive and
It cannot complete meiosis, final abortion；So that ovule in ovary is deformed the differentiation for terminating blastular simultaneously and can not be solid.
Inhibit bud differentiation and Seed Development using vegetable seeds developmental inhibitors the invention discloses a kind of, prevents seed
It generates and diffusion is spread, reach the technology for administering weeds.The controlling object is Alien Weeds Eupatorium adenophorum, Canadian solidago plant
Flower, Horseweed Herb, artemisiifolia, false stinkweed, bidentis, annual fleabane herb, Radix Conyzae sumatrensis, spring bitter fleabane, Ageratum conyzoides, guayule rubber, fragrant eupatorium herb, common vetch
Camomile.
The vegetable seeds developmental inhibitors that the present invention filters out are low using concentration, environmentally safe.Currently, not having still both at home and abroad
Report in relation to studying Eupatorium adenophorum seed inhibitor, the research of this respect is to solving entering to invade to prevent and kill off and providing one for the grass
Road new way.
Specific embodiment
Embodiment 1:
The vegetable seeds inhibitor processing of experimental design is applied alone for glyphosate, can add this field skill using existing method
Aqua is made in auxiliary agent well known to art personnel and water, takes about 100 grams of (α-sodium olefin sulfonates 40 of 490 grams of glyphosate potassiums, adding assistants
Gram, 55 grams of alkyl glycosides, 5 grams of Tea Saponin and a small amount of defoaming agent), be configured to 1 liter of medical fluid with water, do not add or add urea 5g
Sucrose 25g (30g nutritional ingredient being added in 1L medicament, the weight ratio of urea and sucrose is 1:5), urea 25g sucrose 75g, urea
50g sucrose 125g/ liter, is dissolved in 1000 liters of tap water, in the Eupatorium adenophorum bud idiophase of basin alms bowl plantation, respectively with above 4
A different ratio is carried out with 0.5% (glyphosate containing 5g in 1L medicament) and 0.08% (glyphosate containing 0.8g in 1L medicament) concentration
Spraying treatment, if 4 repetitions, sprayer when application, by 80ml/m2Sprinkling.When fruit maturation, when pappus will be unfolded, often
Strain harvests the achene of 10 capitulums respectively, and by achene is received, full is calculated as solid seed, with capitulum little Hua average
Compared to calculating single-strain fructification yield.Then, every plant of seed is individually placed in the culture dish for being lined with filter paper, 25 DEG C of cultures are periodically seen
Germination seed number is examined and counted, until no longer sprouting, calculates single germination rate.Setting percentage and germination rate the detection knot respectively handled
Fruit is as follows:
The results show that 0.5% concentration processing setting percentage be respectively 5% or under, germination rate zero.And concentration is
0.08%, setting percentage is respectively germination rate 35.31%, 13.25%, 3.75%, 0.25%, germination rate is then respectively 13.25%,
2.5%, 0%, 0%, 0%.Obviously, glyphosate addition urea 5g sucrose 25g, urea 25g sucrose 75g, urea 50g sucrose
125g/ liter has the effect for significantly inhibiting solid, is properly added urea and sucrose, can dramatically increase its inhibitory effect.But
It is that glyphosate is applied alone, needs higher position reason concentration that can reach good inhibitory effect.Therefore, urea and sugarcane are properly added
Sugar realizes glyphosate at low concentrations to weed seed inhibiting effect.
Embodiment 2:
The vegetable seeds inhibitor processing of experimental design is applied alone for glyphosate, takes 490 grams of glyphosate potassiums, adding assistants about
100 grams (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, 5 grams of Tea Saponin and a small amount of defoaming agent), it is configured to 1 liter of medical fluid with water,
Do not add or add ammonium hydrogen carbonate 15g sucrose 25g, ammonium hydrogen carbonate 25g sucrose 75g, ammonium hydrogen carbonate 50g sucrose 125g/ liter,
It is dissolved in 1000 liters of tap water, in the Eupatorium adenophorum bud idiophase of basin alms bowl plantation, respectively with above 4 different ratios, with
0.5% and 0.08% concentration carries out spraying treatment, if 4 repetitions, sprayer when application, by 80ml/m2Sprinkling.To fruit at
When ripe, when pappus will be unfolded, every plant of achene for harvesting 10 capitulums respectively, by achene is received, full is calculated as solid seed,
Single-strain fructification yield is calculated compared with capitulum little Hua average.Then, every plant of seed is individually placed in the culture for being lined with filter paper
In ware, 25 DEG C of cultures, routine observation simultaneously counts germination seed number, until no longer sprouting, calculates single germination rate.Respectively handle
Setting percentage and germination rate testing result are as follows:
The results show that 0.5% concentration processing setting percentage be respectively 5% or so or under, germination rate zero.And it is dense
Degree is 0.08%, and setting percentage is respectively germination rate 37.25%, 15.67%, 9.13%, 8.25%, and germination rate is then respectively
14.25%, 4.25%, 1.25%, 0.Obviously, glyphosate addition ammonium hydrogen carbonate 15g sucrose 25g, ammonium hydrogen carbonate 25g sucrose
75g, ammonium hydrogen carbonate 50g sucrose 125g/ liter have the effect for significantly inhibiting solid, be properly added ammonium hydrogen carbonate and sucrose, can
To dramatically increase its inhibitory effect.But glyphosate is applied alone, and needs higher position reason concentration that can reach good inhibition effect
Fruit.Therefore, it is properly added ammonium hydrogen carbonate and sucrose, realizes glyphosate at low concentrations to weed seed inhibiting effect.
Embodiment 3:
The vegetable seeds inhibitor processing of experimental design be glyphosate be applied alone and with ethephon (CEPHA),2-(chloroethyl) phosphonic acid by 20:1,10:1,5:1 and
The weight ratio compounding agent of 4:1 is handled, the combination for taking 490 grams of glyphosate potassiums or glyphosate potassium to compound in proportion with ethephon (CEPHA),2-(chloroethyl) phosphonic acid
About 100 grams of object, adding assistant (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, 5 grams of Tea Saponin and a small amount of defoaming agent), is configured to
1 liter of medical fluid, addition urea 25g sucrose 75g (100g nutritional ingredient is added in 1L medicament, the weight ratio of urea and sucrose is 1:
3), be dissolved in 1000 liters of tap water, basin alms bowl plantation the Eupatorium adenophorum bud idiophase, respectively with above 4 different ratios with
0.1% concentration carries out spraying treatment, if 4 repetitions, sprayer when application, by 80ml/m2Sprinkling.When fruit maturation, hat
Hair be when will be unfolded, every plant of achene for harvesting 10 capitulums respectively, and by achene is received, full is calculated as solid seed, with head
Inflorescence little Hua average is compared to calculating single-strain fructification yield.Then, every plant of seed is individually placed in the culture dish for being lined with filter paper, 25
DEG C culture, routine observation simultaneously counts germination seed number, until no longer until sprouting.
Setting percentage is respectively 12.25%, 3.75%, 2.75%, 19.33%, 28.35% as the result is shown, and germination rate then divides
It Wei 5.35%, 0%, 0%, 5.25%, 7.25%.Obviously, glyphosate be applied alone and with ethephon (CEPHA),2-(chloroethyl) phosphonic acid by 20:1,10:1,5:1 and
4:1 compounding agent has the effect for significantly inhibiting solid, is properly added a small amount of ethephon (CEPHA),2-(chloroethyl) phosphonic acid, and glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid press 20:1,10:
1 ratio compounding can dramatically increase its inhibitory effect.
Embodiment 4:
Take about 100 grams of 490 grams of glyphosate potassiums, adding assistants (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, Tea Saponin 5
Gram and a small amount of defoaming agent), be configured to 1 liter of medical fluid of vegetable seeds inhibitor medicament with water, add urea 15g/ sucrose 35g, no
Addition or respectively add peach gum, xanthan gum, gelatin, Arabic gum, dextrin, starch, agar, each 5g of carboxymethyl cellulose and
45g；Concentration for the treatment of is 0.09%, in the Eupatorium adenophorum bud idiophase of basin alms bowl plantation, respectively with above 5 different ratios
0.09% concentration carries out spraying treatment, if 4 repetitions, sprayer when application, by 80ml/m2Sprinkling.When fruit maturation, hat
Hair be when will be unfolded, every plant of achene for harvesting 10 capitulums respectively, and by achene is received, full is calculated as solid seed, with head
Inflorescence little Hua average is compared to calculating single-strain fructification yield.Then, every plant of seed is individually placed in the culture dish for being lined with filter paper, 25
DEG C culture, routine observation simultaneously counts germination seed number, until no longer until sprouting.Setting percentage and germination rate the detection knot respectively handled
Fruit is as follows:
Obviously, inhibitor adds the effect difference of different adhesives, and peach gum can dramatically increase inhibitor and inhibit solid
Effect.And increase with concentration, effect also significantly improves.
Embodiment 5:
Take about 100 grams of 490 grams of glyphosate potassiums, adding assistants (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, Tea Saponin 5
Gram and a small amount of defoaming agent), be configured to medical fluid with water and be made 1 liter, add urea 15g/ sucrose 35g, peach gum 45g be made vegetable seeds suppression
Preparation medicament, by vegetable seeds inhibitor medicament configuration at 1% mother liquor, be then gradually diluted to 1%, 0.25%,
0.0625%, 0.016%, 0.004%, 0.001%, the control of totally 6 concentration and clear water (0%), in the purple stem of basin alms bowl plantation
Herba Lycopi flower bud differentiation period carries out spraying treatment with the above 10 different concentration respectively, and each concentration sets 4 repetitions, when application
With sprayer, by 80ml/m2Sprinkling.Routine observation formalness changes after application.When fruit maturation, when pappus will be unfolded,
Every plant of achene for harvesting 10 capitulums respectively, the wherein achene of artemisiifolia harvest complete stool, by achene is received, full being calculated as is solid
Seed calculates single-strain fructification yield compared with capitulum little Hua average.Then, every plant of seed is individually placed in and is lined with filter paper
In culture dish, 25 DEG C of cultures, routine observation simultaneously counts germination seed number, until no longer sprouting.There is suspend mode for artemisiifolia etc.
The seed of characteristic detects after placing half a year.
When vegetable seeds inhibitor concentration for the treatment of is 1%, three developmental stages of Eupatorium adenophorum are spent by serious suppression
System, although having a small amount of Seed Development when blooming peak period application cannot sprout.When concentration is 0.25%, at the first two
Developmental stage application does not have Seed Development, but peak period application of blooming has Seed Development, and the average hip number of every 10 inflorescences reaches
118, germination percentage 6.3%；When concentration is 0.0625%, 0.016%, flower bud differentiation period application, every 10 inflorescences are put down
Equal hip number is respectively 13.3,26.3, and compareing is 638, and germination percentage is respectively 2.5%, 3.4%, and compareing is 82.5%；
The application of megaspore and microspore cytological development phase, the average hip number of every 10 inflorescences is respectively 89.7,84, and compareing is 596, germination percentage
Respectively 3.7%, 3.6%, compareing is 85%；In peak period application of blooming, the average hip number of every 10 inflorescences is respectively 176,
255, compareing is 691, and germination percentage is respectively 11.5%, 22.6%, and compareing is 82.5%.Concentration be 0.004%,
When 0.001%, flower bud differentiation period application, the average hip number of every 10 inflorescences is respectively 53.6,76.7, and compareing is 653,
Its germination percentage is respectively 6.5%, 78.9%, and compareing is 84.1%；The application of megaspore and microspore cytological development phase, the average knot of every 10 inflorescences
Actual quantities is respectively 79.7,125, and compareing is 609, and germination percentage is respectively 45.7%, 65.2%, and compareing is 84.5%；?
Peak period of blooming application, the average hip number of every 10 inflorescences is respectively 275,550, and compareing is 691, and germination percentage is respectively
12.6%, 25.3%, compareing is 83.5%.
Embodiment 6:
The plant growth regulator glyphosate and its esters glyphosate isopropyl amine salt of experimental design, ammonium glyphosate,
Glyphosate sylvite, glyphosate sodium salt, glyphosate dimethylamine salt take about 100 grams of (α-alkene of 490 grams of glyphosate potassiums, adding assistants
40 grams of base sodium sulfonate, 55 grams of alkyl glycosides, 5 grams of Tea Saponin and a small amount of defoaming agent), it is configured to 1 liter of medical fluid with water, adds urea 15g/
Sucrose 35g, peach gum 45g；It is diluted to concentration for the treatment of 0.1%, 0.05%, 0.025%, 0.0125%, 0.00625% respectively, often
Kind dosages distribution processing sprays 2m2Area Solidago Canadensis, spraying with sprayer, spray amount to be sprayed onto completely, no
Preferably respray.Test is selected in Nanjing Suburban progress, and October 3, weather is fine, and 4 grades of wind-force.Solidago Canadensis is in bud
Idiophase.It is compareed with water.
When fruit maturation, when pappus will be unfolded, every plant of achene for harvesting 10 capitulums respectively is satisfied by achene is received
Full is calculated as solid seed, and single-strain fructification yield is calculated compared with capitulum little Hua average.Then, every plant of seed is individually set
In the culture dish for being lined with filter paper, 25 DEG C of cultures, routine observation simultaneously counts germination seed number, until no longer sprouting.Everywhere
Setting percentage testing result such as the following table 3 of reason.Each ingredient increases with concentration for the treatment of, and inhibitory effect obviously increases, when concentration is
It can achieve acceptable effect when 0.025% or more, further decrease concentration, then it is ineffective.In short, glyphosate
And its esters all has apparent inhibitory effect.
The inhibitory effect of the different glyphosates of table 3. and its esters processing Solidago Canadensis compares
Embodiment 7:
Take about 100 grams of 490 grams of glyphosate potassiums, adding assistants (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, Tea Saponin 5
Gram and a small amount of defoaming agent), be configured to 1 liter of medical fluid with water, add urea 15g, sucrose 35g, peach gum 45g, vegetable seeds is inhibited
Agent medicament configuration is then gradually diluted to 0.25%, 0.0625%, 0.016%, 0.004%, 0.001% at 1% mother liquor,
The control of totally 5 concentration and clear water (0%), Eupatorium adenophorum, Solidago Canadensis, Horseweed Herb, artemisiifolia, vacation in the plantation of basin alms bowl
The bud stages such as stinkweed, bidentis, annual fleabane herb, Radix Conyzae sumatrensis, spring bitter fleabane, Ageratum conyzoides, guayule rubber, fragrant eupatorium herb, Mikania micrantha, respectively
Spraying treatment is carried out with the above 6 different concentration, and each concentration sets 4 repetitions, sprayer when application, by 130ml/m2Spray
It spills.Routine observation formalness changes after application.When fruit maturation, when pappus will be unfolded, every plant harvests 10 heads respectively
The achene of inflorescence, the wherein achene of artemisiifolia harvest complete stool, by achene is received, full is calculated as solid seed, with capitulum little Hua
Average is compared to calculating single-strain fructification yield.Then, every plant of seed is individually placed in the culture dish for being lined with filter paper, 25 DEG C of cultures,
Routine observation simultaneously counts germination seed number, until no longer sprouting.There is the seed of hibernation feature for artemisiifolia etc., places half a year
After detect.
When inhibitor concentration is 0.25%, the differentiation of Eupatorium adenophorum bud bud is suppressed, capitulum 11- after administration
It can exist within 12 days, the then atrophy of inflorescence elder generation chlorosis, young leaflet tablet is sallow at the top of plant.When concentration is 0.0625% or less
It does not have much affect apparently to plant, but bud differentiation is inhibited by different degrees of, only only a few can continue to develop, and by
To the inflorescence feature that observation and expression goes out from the appearance of inhibition are as follows: the diameter of capitulum does not change, color is gradually from depth
Green-pale green-is yellowish green, still, can largely blossom and bear fruit.And the capitulum for compareing strain continues to break up, and knot of blooming
It is real.Pappus can be opened normally.
Setting percentage statistical result such as table
As can be seen from the table, inhibition of the inhibitor concentration at 0.016% or higher, to the seed-setting of above-mentioned weeds
Rate reaches 60% or more.And when concentration is 0.004%, inhibiting rate may also reach up 40% or more, some weeds still may be used
To reach 60% or more.
The plantation germination percentage respectively handled is as follows:
It is as shown in the table, and the germination rate of the solid seed of inhibitor 0.004% and higher concentration is 50% hereinafter, remove artemisiifolia
Outside, below 40%.Great-hearted seed amount can be effectively reduced, is reached by reducing grain weight control or delaying above-mentioned
The purpose of weeds diffusion sprawling.
Embodiment 8:
Take about 100 grams of 490 grams of glyphosate potassiums, adding assistants (40 grams of α-sodium olefin sulfonate, 55 grams of alkyl glycosides, Tea Saponin 5
Gram and a small amount of defoaming agent), be configured to 1 liter of medical fluid, urea 15g, sucrose 35g, peach gum 45g added, by vegetable seeds inhibitor medicine
Agent is configured to 1% mother liquor, be then gradually diluted to 1%, 0.5%, 0.25%, 0.125%, 0.0625%, 0.0313%,
0.016%, 0.008%, 0.004%, 0.002%, the control of totally 10 concentration and clear water (0%), in the purple stem of basin alms bowl plantation
Herba Lycopi flower bud differentiation period carries out spraying treatment with the above 10 different concentration respectively, and each concentration sets 4 repetitions, when application
With sprayer, by 80ml/m2Sprinkling.Routine observation formalness and internal structure develop two aspects after application.Internal structure
Observation paraffin method.Every 3 days sampling treated buds after application, FAA fixer and carnoy fluid are fixed, by de-
Water --- paraffin embedding --- slice --- dyeing --- processes such as film-making, slice thickness are 8 μm, are dyed through siderotil-hematoxylin,
Gummy mounting.Observation structure under the microscope.
Influence of the inhibitor to the Eupatorium adenophorum bud idiophase: when inhibitor concentration is 1%, the differentiation of Eupatorium adenophorum bud
Completely suppressed, capitulum starts to wilt after administration for 11-12 days then to dry up, and Eupatorium adenophorum entirety plant has dead show of withering
As；Concentration also completely inhibits the further development of bud differentiation, the then atrophy of inflorescence elder generation chlorosis, plant when being 0.5%, 0.25%
Top young leaflet tablet is sallow.Concentration is 0.125% and do not have much affect when following to plant, but bud differentiation almost all by
To inhibition, only only a few can continue the inflorescence developed, and the be suppressed feature that observation and expression goes out from the appearance are as follows: cephaloid
The diameter of sequence does not change, color is gradually yellowish green from bottle green-pale green-.And the capitulum for compareing strain continues to break up.
Eupatorium adenophorum internal structure development change: when concentration is 0.25%, the capitulum in bud differentiation period,
Its every colored Pistil And Stamen cell disconnects after administration, and atrophy is at linear；Head in megaspore and microspore cytological development period
Inflorescence, every colored anther cell and petal cell first disconnect after administration, and whole flower is in cross section when further developing
Vestiges are only stayed, bract cell is also severely deformed.Until influence of the concentration to florescence internal structure is relatively slow below 0.125% concentration
With.In Eupatorium adenophorum development process, at pollen mother cell period the volume of its cell and core increase, each coyote hole
Four layers of wall closely connect, tapetum is apocyte, and during subsequent meiosis, tapetum remains at original position
The development for being set to pollen mother cell provides nutrition, and until the tetrad of pollen grain, tapetum just starts to degenerate, monokaryon pollen
Grain period is still it is observed that the tapetum just degenerated.The middle layer of the tapetal cell and anther wall that more than 0.004% concentration handle
Cell separation, and invade coyote hole and gradually surrounded to pollen mother cell, the abortion of 5 anther is only stayed in spending to later period every of development
Vestiges.While anther development is obstructed, the ovule in ovary also deforms not the differentiation that can be carried out blastular.
Embodiment 8:
Break up period (i.e. flower head diameter is in 2-4mm) in Eupatorium adenophorum bud, with the plant prepared in embodiment 5
Seed inhibitor medicament carries out sprinkling processing, concentration for the treatment of 0.125%, 0.08%, sprayer when application, by 80ml/m2Spray
Medical fluid is spilt, if 3 repetitions, each replicated plot area is 30m2, place is area --- the Kunming, Yunnan that Eupatorium adenophorum is spread unchecked
Heilongtang park.Fair weather, processing in afternoon.
Capitulum develops into fruit completely, when pappus starts to spread out, collects fruit.The setting percentage of two concentration for the treatment of point
Not Wei 5.6% and 23.67%, germination rate then be respectively 0% and 5.25%.With significant inhibitory effect.
Embodiment 9:
At Mikania micrantha bud differentiation period (i.e. flower head diameter is in 3-5mm), with the plant species prepared in embodiment 5
Sub- inhibitor medicament carries out sprinkling processing, concentration for the treatment of 0.125%, 0.08%, sprayer when application, by 80ml/m2Sprinkling
Medical fluid, if 3 repetitions, each replicated plot area is 5m2, place is Shenzhen City, Guangdong Province Mt Wutongshan.Fair weather, at afternoon
Reason.
Capitulum develops into fruit completely, when pappus starts to spread out, collects fruit.The setting percentage of two concentration for the treatment of point
Not Wei 15.98% and 35.56%, germination rate then be respectively 5.33% and 15.67%.With significant inhibitory effect.
Claims (18)
1. a kind of vegetable seeds developmental inhibitors, it is characterised in that: the vegetable seeds developmental inhibitors include plant growth
Regulator and water；The main component of the plant growth regulator is glyphosate；The vegetable seeds developmental inhibitors further include battalion
It forms point and sticker；
The weight ratio of the plant growth regulator and the nutritional ingredient is 490:0.5 ~ 300；
The weight ratio of the plant growth regulator and the binder is 490:1 ~ 150；
The sticker is selected from least one of peach gum, Arabic gum, agar, carboxymethyl cellulose；The nutrition at
It is selected from least one of sucrose, urea and ammonium hydrogen carbonate.
2. vegetable seeds developmental inhibitors according to claim 1, it is characterised in that: the plant growth regulator with
The weight ratio of the nutritional ingredient is 490:1 ~ 250；The weight ratio of the plant growth regulator and the binder is
490:1 ~ 100.
3. vegetable seeds developmental inhibitors according to claim 2, it is characterised in that: the plant growth regulator with
The weight ratio of the nutritional ingredient is 490:10 ~ 250；The weight ratio of the plant growth regulator and the binder is
490:2 ~ 100.
4. vegetable seeds developmental inhibitors according to claim 3, it is characterised in that: the plant growth regulator with
The weight ratio of the nutritional ingredient is 490:30 ~ 200；The weight ratio of the plant growth regulator and the binder is
490:5 ~ 50.
5. any vegetable seeds developmental inhibitors according to claim 1 ~ 4, it is characterised in that: the nutritional ingredient
Concentration in the vegetable seeds developmental inhibitors is 0.05% ~ 30%；The sticker is in the vegetable seeds developmental inhibitors
In concentration be 0.01% ~ 15%.
6. vegetable seeds developmental inhibitors according to claim 5, it is characterised in that: the nutritional ingredient is in the plant
Concentration in seed development inhibitor is 3% ~ 22%；Concentration of the sticker in the vegetable seeds developmental inhibitors is
0.02%~10%。
7. vegetable seeds developmental inhibitors according to claim 1, it is characterised in that: the nutritional ingredient be urea and
Sucrose, or be ammonium hydrogen carbonate and sucrose；The sticker is peach gum.
8. vegetable seeds developmental inhibitors according to claim 7, it is characterised in that: the weight of the urea and sucrose
Than for 1:1 ~ 1:5, the weight ratio of the ammonium hydrogen carbonate and sucrose is 1:1 ~ 1:5.
9. vegetable seeds developmental inhibitors according to claim 1, it is characterised in that: the glyphosate is glyphosate
And its esters.
10. vegetable seeds developmental inhibitors according to claim 9, it is characterised in that: the glyphosate salt is grass
At least one in sweet phosphine isopropyl amine salt, ammonium glyphosate, glyphosate sylvite, glyphosate sodium salt and glyphosate dimethylamine salt
Kind.
11. vegetable seeds developmental inhibitors according to claim 1, it is characterised in that: the plant growth regulator
In further include ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
12. vegetable seeds developmental inhibitors according to claim 11, it is characterised in that: the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Weight ratio >=3:1.
13. vegetable seeds developmental inhibitors according to claim 12, it is characterised in that: the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Weight ratio is 50:1 ~ 4:1.
14. vegetable seeds developmental inhibitors according to claim 13, it is characterised in that: the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Weight ratio is 20:1 ~ 5:1.
15. vegetable seeds developmental inhibitors according to claim 14, it is characterised in that: the glyphosate and ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Weight ratio is 20:1 ~ 10:1.
16. application of the vegetable seeds developmental inhibitors described in claim 1 in controlling weeds.
17. application according to claim 16, it is characterised in that: the vegetable seeds developmental inhibitors is taken to be configured to plant
Growth regulator is that 0.25%~0.004% concentration expressed in percentage by weight sprays, and puts Sheng to the flowers are in blossom for weeds flower bud differentiation period using the time
Phase.
18. application according to claim 16 or 17, it is characterised in that: the weeds are farmland and Alien Weeds purple stem
Herba Lycopi, Solidago Canadensis, Horseweed Herb, artemisiifolia, false stinkweed, bidentis, annual fleabane herb, Radix Conyzae sumatrensis, spring bitter fleabane, victory are red
Ji, guayule rubber, fragrant eupatorium herb, Mikania micrantha.
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| CN109757248B (en) * | 2019-03-25 | 2021-06-01 | 西南林业大学 | Method for inhibiting flowering of mikania micrantha by using combined exogenous hormone |
| CN110537553A (en) * | 2019-09-20 | 2019-12-06 | 贵州九州农牧发展有限公司 | Control agent for broad-leaved weeds in capsicum field |
| CN112913852A (en) * | 2021-02-02 | 2021-06-08 | 中国科学院华南植物园 | Efficient prevention and control method for aircraft grass and south American wedelia chinensis |
| CN112889850B (en) * | 2021-02-10 | 2021-12-14 | 广东省农业科学院农业生物基因研究中心 | Rice pre-harvest sprouting inhibitor prepared from south medicinal herb extract as well as preparation method and application thereof |
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| CN101743995A (en) * | 2009-12-23 | 2010-06-23 | 深圳诺普信农化股份有限公司 | Efficiency glyphosate herbicidal composition |
| CN101938907A (en) * | 2008-01-25 | 2011-01-05 | 辛根塔有限公司 | Herbicidal compositions |
| CN102578140A (en) * | 2012-01-05 | 2012-07-18 | 南京登博生物科技有限公司 | Plant growth and development inhibitor and application thereof |
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| CN101564044B (en) * | 2009-05-19 | 2012-07-25 | 美丰农化有限公司 | Soluble granule of glyhosate ammonium and preparation method |
| CN104886121B (en) * | 2015-07-08 | 2018-01-30 | 山东潍坊润丰化工股份有限公司 | A kind of glyphosate dry suspending agent, its preparation method and its application |
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| CN101938907A (en) * | 2008-01-25 | 2011-01-05 | 辛根塔有限公司 | Herbicidal compositions |
| CN101743995A (en) * | 2009-12-23 | 2010-06-23 | 深圳诺普信农化股份有限公司 | Efficiency glyphosate herbicidal composition |
| CN102578140A (en) * | 2012-01-05 | 2012-07-18 | 南京登博生物科技有限公司 | Plant growth and development inhibitor and application thereof |
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