CN109679661A - Galenical and preparation method thereof - Google Patents
Galenical and preparation method thereof Download PDFInfo
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- CN109679661A CN109679661A CN201910126171.1A CN201910126171A CN109679661A CN 109679661 A CN109679661 A CN 109679661A CN 201910126171 A CN201910126171 A CN 201910126171A CN 109679661 A CN109679661 A CN 109679661A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 58
- 150000001875 compounds Chemical class 0.000 claims abstract description 35
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000000813 microbial effect Effects 0.000 claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 17
- 239000011574 phosphorus Substances 0.000 claims abstract description 17
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- 241000186660 Lactobacillus Species 0.000 claims abstract description 15
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 15
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 15
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 15
- 241001446247 uncultured actinomycete Species 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 235000010419 agar Nutrition 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 235000012907 honey Nutrition 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 26
- 230000000052 comparative effect Effects 0.000 description 36
- 241000196324 Embryophyta Species 0.000 description 24
- 239000002689 soil Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 12
- 238000010790 dilution Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- 241000207199 Citrus Species 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 235000020971 citrus fruits Nutrition 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 240000008436 Ipomoea aquatica Species 0.000 description 7
- 235000019004 Ipomoea aquatica Nutrition 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 235000013311 vegetables Nutrition 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
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- 238000009472 formulation Methods 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- KAATUXNTWXVJKI-UHFFFAOYSA-N cypermethrin Chemical class CC1(C)C(C=C(Cl)Cl)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 KAATUXNTWXVJKI-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 235000021317 phosphate Nutrition 0.000 description 3
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000011814 protection agent Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 240000006108 Allium ampeloprasum Species 0.000 description 2
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 241001478315 Candidatus Liberibacter asiaticus Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 241000830536 Tripterygium wilfordii Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000008429 bread Nutrition 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 238000005341 cation exchange Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 235000015398 thunder god vine Nutrition 0.000 description 2
- 238000009333 weeding Methods 0.000 description 2
- YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- SFMJNHNUOVADRW-UHFFFAOYSA-N n-[5-[9-[4-(methanesulfonamido)phenyl]-2-oxobenzo[h][1,6]naphthyridin-1-yl]-2-methylphenyl]prop-2-enamide Chemical compound C1=C(NC(=O)C=C)C(C)=CC=C1N1C(=O)C=CC2=C1C1=CC(C=3C=CC(NS(C)(=O)=O)=CC=3)=CC=C1N=C2 SFMJNHNUOVADRW-UHFFFAOYSA-N 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 229940046001 vitamin b complex Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of preparation methods of galenical, comprising the following steps: is uniformly mixed actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora and growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~16.3:7.5~8.0:12~13 and forms microbial compound preparation；The canned fermentation of stirring 3~10 days is packed into after microbial compound preparation, culture medium, sugar, high protein and water are mixed according to 3~4:5 of mass ratio~6:10~11:5.5~6.5:70~80, wherein, 0~40 degree Celsius of fermentation temperature is controlled in fermentation process, obtains galenical.
Description
Technical field
The present invention relates to a kind of galenicals and preparation method thereof.
Background technique
Soil remediation currently on the market is substantially through organic fertilizer and realizes various functions, although many kinds of function
It can be single.It can not solve the problems such as hardened, agriculture is residual exceeded in soil.It is unfavorable for plant growth in the long run.
Summary of the invention
The present invention provides a kind of galenicals and preparation method thereof, can effectively solve the above problems.
The present invention is implemented as follows:
A kind of preparation method of galenical, comprising the following steps:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora with
And growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~
16.3:7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation；
S2, by microbial compound preparation, culture medium, sugar, high protein and water according to 3~4:5 of mass ratio~6:10~11:
5.5~6.5:70~80 is packed into the canned fermentation of stirring 3~10 days after mixing, wherein fermentation temperature 0~40 is controlled in fermentation process
Degree Celsius, obtain galenical.
The present invention also provides a kind of galenicals obtained by the above method.
The beneficial effects of the present invention are: the present invention provide described galenical and preparation method thereof can effectively decomposing pesticide it is residual
It stays and increases the cation exchange capacity (CEC) in soil simultaneously.In addition, the galenical can also activate soil, soil is allowed to build again
Ecological chain is erected, and allows beneficial bacterium to kill the disinfect pathogen in soil, and mend bacterium in due course, neutralizes the pH value of soil, promotes to plant
Absorption of the object to ASI systematic approach, while the nutrient in air is absorbed, it is converted to the nutritional ingredient for plant absorption, this side
Method is at low cost, and can accomplish to cultivate year after year.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention
Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to range, it for those of ordinary skill in the art, without creative efforts, can be with root
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the preparation method flow chart of microbial compound preparation provided in an embodiment of the present invention.
Fig. 2 is the preparation method flow chart of biological plant protection agent provided in an embodiment of the present invention.
Fig. 3 is the preparation method flow chart of biological agent provided in an embodiment of the present invention.
Fig. 4 is the method flow diagram of prevention and treatment Citrus Huanglongbing pathogen provided in an embodiment of the present invention.
Fig. 5 is the preparation method flow chart of animal nutrient solution provided in an embodiment of the present invention.
Specific embodiment
To keep the purposes, technical schemes and advantages of embodiment of the present invention clearer, implement below in conjunction with the present invention
The technical solution in embodiment of the present invention is clearly and completely described in attached drawing in mode, it is clear that described reality
The mode of applying is some embodiments of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ability
Domain those of ordinary skill every other embodiment obtained without creative efforts, belongs to the present invention
The range of protection.Therefore, the detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit below and is wanted
The scope of the present invention of protection is sought, but is merely representative of selected embodiment of the invention.Based on the embodiment in the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts belongs to this
Invent the range of protection.
The alkaline condition that it is 8.5 or so in PH that nitrogen fixation flora, which is most suitable for, and temperature is numerous under 20~22 DEG C of growing environment
It grows, and generates the growth substance of the substances such as organic nitrogen, Amino acid.The alkaline condition that it is 9.0 or so in PH that Soluble phosphorus flora, which is most suitable for,
And temperature is breeds under 22~24 DEG C of growing environment, can dissolve ineffectivity phosphorus enables it to be transformed into the phosphorus element that plant utilizes, point
The excellent physical properties of soil can be increased by secreting polysaccharide material out.Its slightly acidic environment for being suitable for PH6.5 or so of nitric acid flora
Lower growth, and the bread worm is about at 30 DEG C, and can be nitric nitrogen by toxic ammonia for plant absorption.Photosynthetic bacteria group its be suitable for
It is grown under the slightly acidic environment of PH6.0 or so, and the bread worm is about at 25 DEG C, and can produce many acitve organic matter matter, such as
The antiviral active substances such as vitamin B complex, folic acid, antiviral substance and positive growth factor.Lactobacillus its be suitable for PH4.0
The acidity of left and right, and growth temperature can secrete lactic acid brake bacteria factor and antibacterial molecule and make with very strong sterilization about at 35 DEG C
With.Its acidity for being suitable for PH5.0 or so of yeast flora, and growth temperature can secret out of promotion cell division about at 37 DEG C
B family vitamin abundant, the substances such as glucose.Its neutral environment for being suitable for PH7.0 or so of actinomycete group, and growth temperature
About at 30 DEG C, decomposable rotten organic matter is simultaneously converted into the nutriment for being conducive to biological growth, and secretes antibiotic etc. and is living
Property antibacterial substance.It is suitable for neutral environment to growth flora, and growth temperature can be secreted about at 26 DEG C and be conducive to animal and plant
The hormone of growth.
Since the growing environment of this above-mentioned 8 big flora is different, if proportion is improper, phase can be generated between various floras
Mutually inhibit, the synergistic effect for making it be difficult to play various floras.
The present invention through a large number of experiments, compounds out the microbial compound preparation that can be used for soil modification, and the microorganism is multiple
Closing preparation has the characteristics that preparation method is simple, and mutually coordinated, mutual can restrict between 8 big floras so as to significantly change
Kind soil property, so as to for crops or vegetable fertilizer and biological pesticide.And be prepared by microbial compound preparation
Animal nutrient solution, the healthy growth that the immunity of animal can also be improved, improve digestion and absorption function, promote animal, specifically such as
Under.
Shown in referring to Fig.1, the embodiment of the present invention provides a kind of preparation method of microbial compound preparation, including following step
It is rapid:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora with
And growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~
16.3:7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation.
As further improved, in step sl, it is preferred that the actinomycete group, the nitrogen fixation flora, described photosynthetic
The matter of flora, the nitric acid flora, the lactobacillus, the yeast flora, the Soluble phosphorus flora and the growth flora
Measure ratio are as follows: 14:8.5:16:7.3:18:16:7.7:12.5.
The embodiment of the present invention also provides a kind of microbial compound preparation being prepared by the above method.
Embodiment 1-1: by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, molten
Phosphorus flora and growth flora are uniformly mixed that form microorganism multiple according to mass ratio 14:8.5:16:7.3:18:16:7.7:12.5
Close preparation.It is test vegetables with water spinach, carries out soil cultivation, and apply the microbial compound preparation, average cultivation temperature is
25 ± 2 DEG C, the plant strain growth situation of each processing group is measured when 30 days after the emergence of hollow vegetable seedling, 3 groups of parallel tests is tested, makes even
Mean value.
Embodiment 1-2: by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, molten
Phosphorus flora and growth flora are uniformly mixed according to mass ratio 13.8:8.3:15.8:7:17.7:15.8:7.5:12 forms micro- life
Object compound formulation.It is test vegetables with water spinach, carries out soil cultivation, and apply the microbial compound preparation, average culture temperature
Degree is 25 ± 2 DEG C, and the plant strain growth situation of each processing group is measured when 30 days after the emergence of hollow vegetable seedling, tests 3 groups of parallel tests,
It is averaged.
Embodiment 1-3: by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, molten
Phosphorus flora and growth flora according to mass ratio 14.3:8.8:16.3:7.5:18.2:16.3:8.0:13 be uniformly mixed formed it is micro-
Biological compound preparation.It is test vegetables with water spinach, carries out soil cultivation, and apply the microbial compound preparation, it is average to cultivate
Temperature is 25 ± 2 DEG C, and the plant strain growth situation of each processing group is measured when 30 days after the emergence of hollow vegetable seedling, and 3 groups of test tries in parallel
It tests, is averaged.
Comparative example 1-1: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of actinomycete group is
13.5。
Comparative example 1-2: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of actinomycete group is
14.5。
Comparative example 1-3: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of nitrogen fixation flora is
8.0。
Comparative example 1-4: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of nitrogen fixation flora is
9.0。
Comparative example 1-5: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of photosynthetic bacteria group is
15.5。
Comparative example 1-6: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of photosynthetic bacteria group is
16.5。
Comparative example 1-7: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of nitric acid flora is
6.5。
Comparative example 1-8: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of nitric acid flora is
8.0。
Comparative example 1-9: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of lactobacillus is
17.0。
Comparative example 1-10: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of lactobacillus is
18.5。
Comparative example 1-11: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of yeast flora is
15.5。
Comparative example 1-12: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of yeast flora is
16.5。
Comparative example 1-13: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of Soluble phosphorus flora is
7.0。
Comparative example 1-14: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of Soluble phosphorus flora is
8.5。
Comparative example 1-15: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of growth flora is
11.5。
Comparative example 1-16: this comparative example and embodiment 1-1 are essentially identical, the difference is that the ratio of growth flora is
13.5。
Comparative example 1-17: this comparative example and embodiment 1-1 are essentially identical, the difference is that not applying the compound system of microorganism
Agent.
Comparative example 1-18: this comparative example and embodiment 1-1 are essentially identical, the difference is that applying commercially available turf vermiculite
Matrix.
The influence that 1 microbial compound preparation of table grows water spinach
From table 1 it follows that equal in fresh weight and chlorophyll content using the water spinach that microbial compound preparation is cultivated
Higher than control group, illustrate that microbial compound preparation provides enough nutrition for water spinach, further shows that multiple in microorganism
Under the action of closing preparation, nutriment sustained release improves the fertilizer of matrix, and then promotes the growth of water spinach.
Referring to shown in Fig. 2, the embodiment of the present invention further provides for a kind of by mentioned microorganism compound formulation preparation biology
The preparation method of plant protection agent, comprising the following steps:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora with
And growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~
16.3:7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation；
S2, by diatomite, natural plants material extracting solution and microbial compound preparation according to 85~88:7.5 of mass ratio~
8.5:5.5~6.5 ratio is mixed.
In step s 2, it is preferred that diatomite, natural plants material extracting solution and the microbial compound preparation are pressed into matter
Measure the ratio mixing than 86:8:6.In addition, the material of the natural plants material extracting solution is selected from weeding chrysanthemum, capsicum, leek, Cortex Phellodendri
And the extracting solution of the mixtures such as tripterygium wilfordii.Specifically, the preparation method of the natural plants material extracting solution the following steps are included:
Weeding chrysanthemum, capsicum, leek, Cortex Phellodendri and tripterygium wilfordii are added S21 according to weight ratio 1:0.1:0.2:0.2:0.1
It is boiled in water 10~20 minutes, it is stand-by after filtering.
By the tiny crystals of minerals, wood louse, aphid, each class pest pore and skin can be hurt, in favor of beneficial bacterium
Group's intrusion absorbs the nutriments such as moisture and protein.The distinctive smell that natural botanical extraction liquid is distributed can play insect prevention
Effect.Fortification of plants material and micropopulation are to the drive of the pest effect of going out.
Test proves that the biology plant protection agent is diluted sprinkling to Chinese cabbage and corn, so as to by Chinese cabbage and corn
The disease incidence of middle aphid can drop to 5% and 4% or less by original 30.0% and 20% or so respectively.
Referring to shown in Fig. 3, the embodiment of the present invention further provides for a kind of by mentioned microorganism compound formulation preparation plant
The preparation method of preparation, comprising the following steps:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora with
And growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~
16.3:7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation；
S3, by microbial compound preparation, culture medium, sugar, high protein and water according to 3~4:5 of mass ratio~6:10~11:
5.5~6.5:70~80 is packed into the canned fermentation of stirring 3~10 days after mixing, wherein fermentation temperature 0~40 is controlled in fermentation process
Degree Celsius, obtain galenical.
In step s3, it is preferred that by microbial compound preparation, culture medium, sugar, high protein and water according to mass ratio
The canned fermentation of stirring 5 days is packed into after 3.4:5.4:10.2:5.8:74 mixing, wherein control fermentation temperature 25 in fermentation process and take the photograph
Family name's degree.
The culture medium can be beef extract, peptone, agar, honey and its mixture.
The embodiment of the present invention also provides a kind of galenical obtained by the above method.
The galenical effectively decomposing pesticide residual simultaneously can increase the cation exchange capacity (CEC) in soil simultaneously.Experiment card
Bright, the galenical can be carried out effectively degrading to the pesticide residues such as organic phosphates and cypermethrin class.It is adding
Have in the soil of galenical and tested, galenical is being added after a week, the organic phosphates in soil can reduce by 30% left side
The right side, cypermethrin class can reduce by 40% or so；In addition, the organic phosphates in soil can drop after being added galenical one month
Low 60% or so, cypermethrin class can reduce by 75% or so.
Referring to shown in Fig. 4, the embodiment of the present invention further provides for one kind and passes through above-mentioned galenical and above-mentioned biological plant protection
The method of agent prevention and treatment Citrus Huanglongbing pathogen, comprising the following steps:
S4, every 48~96 hours by the biological plant protection dilution agent after sprinkling and pouring root are carried out to citrus, and at the same time
Pouring root and Xi Shu will be carried out after galenical dilution, and apply efficient organic fertilizer and liquid fertilizer simultaneously, operated continuously 2~4 times；
S5 will carry out sprinkling and pouring root to citrus after the biological plant protection dilution agent after being then spaced 10~20 days, and
Simultaneously pouring root and Xi Shu will be carried out after galenical dilution；
S6, finally every other month with after the biological plant protection dilution agent comprehensively sprinkling, and at the same time by the plant system
Pouring root is carried out after dilution agent.
In step s 4, it is preferred that every 72 hours by the biological plant protection dilution agent after citrus is sprayed and is filled
Root, and at the same time by pouring root and Xi Shu is carried out after galenical dilution, and apply efficient organic fertilizer and liquid fertilizer simultaneously, continuously
Operation 3 times.
In step s 5, it is preferred that interval will be sprayed and be filled to citrus after the biological plant protection dilution agent after 15 days
Root, and at the same time by pouring root and Xi Shu is carried out after galenical dilution.
Trial zone citrus is tested by the above method, yellow twig disease incidence can be by original 10.0% or so
Drop to 0.5% or less.In addition, orange yield by present be averaged 1500kg/ mu, improve to 1826kg/ mus, raising yield 20% with
On.Citrus of long duration in addition to infecting yellow twig, except the basic death of root system, can solve the breaking-out of yellow twig substantially.
Referring to Figure 5, the embodiment of the present invention further provides for a kind of by mentioned microorganism compound formulation preparation animal
The preparation method of nutrient solution, comprising the following steps:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora with
And growth flora according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~
16.3:7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation；
S7, by microbial compound preparation, culture medium, sugar, high protein and water according to 3~4:5 of mass ratio~6:10~11:
21~25:110~120 is packed into the canned fermentation of stirring 3~10 days after mixing, wherein fermentation temperature 0~40 is controlled in fermentation process
Degree Celsius, obtain animal nutrient solution.
In the step s 7, it is preferred that by microbial compound preparation, culture medium, sugar, high protein and water according to mass ratio
The canned fermentation of stirring 5 days is packed into after 3.5:5:10:24:115 mixing, wherein 25 degrees Celsius of fermentation temperature are controlled in fermentation process,
Obtain animal nutrient solution.
The animal nutrient solution that the embodiment of the present invention obtains has special sour taste, dynamic to livestock and poultry such as live pig, broiler chicken, cattle and sheep
Object has preferable palatability, and growth promotion ability is strong, and feed molecular structure becomes smaller, and easily absorbs.After livestock and poultry animal feeds this product,
Its function of intestinal canal has obtained biggish improvement, improves immune efficacy, improves the conversion ratio of feed, has preferable ecology, society
It can benefit.Improve the meats such as live pig, broiler chicken product safety.In addition, adjust intestinal flora balance, kill animal it is intracorporal have fall ill
Bacterium enhances fowl poultry immune systemic disease resistance, effectively prevents the generation of all kinds of epizootic diseases.It is demonstrated experimentally that adding the animal
The feed of nutrient solution, the weight average of 3 months pigs increases by 8% or so, and disease incidence reduces by 10%.The foregoing is merely the present invention
Preferred embodiment, be not intended to restrict the invention, for those skilled in the art, the present invention can have respectively
Kind change and variation.All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should all wrap
Containing within protection scope of the present invention.
Claims (5)
1. a kind of preparation method of galenical, which comprises the following steps:
S1, by actinomycete group, nitrogen fixation flora, photosynthetic bacteria group, nitric acid flora, lactobacillus, yeast flora, Soluble phosphorus flora and life
Long flora is according to 13.8~14.3:8.3 of mass ratio~8.8:15.8~16.3:7~7.5:17.7~18.2:15.8~16.3:
7.5~8.0:12~13 are uniformly mixed and form microbial compound preparation；
S2, by microbial compound preparation, culture medium, sugar, high protein and water according to 3~4:5 of mass ratio~6:10~11:5.5
~6.5:70~80 is packed into the canned fermentation of stirring 3~10 days after mixing, wherein control fermentation temperature 0~40 in fermentation process and take the photograph
Family name's degree, obtains galenical.
2. preparation method according to claim 1, which is characterized in that in step sl, the actinomycete group, the fixed nitrogen
Flora, the photosynthetic bacteria group, the nitric acid flora, the lactobacillus, the yeast flora, the Soluble phosphorus flora and described
Grow the mass ratio of flora are as follows: 14:8.5:16:7.3:18:16:7.7:12.5.
3. preparation method according to claim 1, which is characterized in that in step s 2, by microbial compound preparation, culture
Base, sugar, high protein and water are packed into the canned fermentation of stirring 5 days after mixing according to mass ratio 3.4:5.4:10.2:5.8:74,
In, 25 degrees Celsius of fermentation temperature are controlled in fermentation process.
4. preparation method according to claim 1, which is characterized in that in step s 2, the material of the culture medium includes
Beef extract, peptone, agar, honey and its mixture.
5. a kind of galenical, which is characterized in that the preparation method of galenical any one as described in claim 1-4
It is made.
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| WO2020168650A1 (en) * | 2019-02-20 | 2020-08-27 | 易之泰生物科技（龙岩）有限公司 | Microorganism composite formulation and preparation method therefor |
| CN111621439A (en) * | 2020-06-09 | 2020-09-04 | 誉辰生物科技(福建)有限公司 | Microbial disinfectant and preparation thereof |
| CN114621894A (en) * | 2022-02-17 | 2022-06-14 | 内蒙古亿民生物科技有限公司 | Preparation method of microbial agent and organic fertilizer containing microbial agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020168650A1 (en) * | 2019-02-20 | 2020-08-27 | 易之泰生物科技（龙岩）有限公司 | Microorganism composite formulation and preparation method therefor |
| CN111621439A (en) * | 2020-06-09 | 2020-09-04 | 誉辰生物科技(福建)有限公司 | Microbial disinfectant and preparation thereof |
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