CN109652346B - 锌吸附菌株的应用及锌吸附菌株的培养方法 - Google Patents
锌吸附菌株的应用及锌吸附菌株的培养方法 Download PDFInfo
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- CN109652346B CN109652346B CN201910124971.XA CN201910124971A CN109652346B CN 109652346 B CN109652346 B CN 109652346B CN 201910124971 A CN201910124971 A CN 201910124971A CN 109652346 B CN109652346 B CN 109652346B
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- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 229910052725 zinc Inorganic materials 0.000 title claims abstract description 54
- 239000011701 zinc Substances 0.000 title claims abstract description 54
- 238000001179 sorption measurement Methods 0.000 title claims abstract description 49
- 238000012136 culture method Methods 0.000 title claims description 20
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000004321 preservation Methods 0.000 claims abstract description 16
- 238000009629 microbiological culture Methods 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 30
- 235000015097 nutrients Nutrition 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 23
- 239000012452 mother liquor Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 11
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 11
- 229960001763 zinc sulfate Drugs 0.000 claims description 11
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 11
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 235000015278 beef Nutrition 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 13
- 230000015556 catabolic process Effects 0.000 abstract description 9
- 238000006731 degradation reaction Methods 0.000 abstract description 9
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000003911 water pollution Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 42
- 238000009631 Broth culture Methods 0.000 description 19
- 238000000926 separation method Methods 0.000 description 19
- 230000001580 bacterial effect Effects 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 12
- 239000010802 sludge Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- 239000007788 liquid Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 239000002351 wastewater Substances 0.000 description 9
- 238000012216 screening Methods 0.000 description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 6
- 235000011130 ammonium sulphate Nutrition 0.000 description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 6
- 235000019341 magnesium sulphate Nutrition 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 5
- 238000000576 coating method Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000003723 Smelting Methods 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000013048 microbiological method Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000010801 sewage sludge Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000467496 Pseudomonas protegens Species 0.000 description 1
- 241001464778 Zymobacter Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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CN201910124971.XA CN109652346B (zh) | 2019-02-19 | 2019-02-19 | 锌吸附菌株的应用及锌吸附菌株的培养方法 |
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CN201910124971.XA CN109652346B (zh) | 2019-02-19 | 2019-02-19 | 锌吸附菌株的应用及锌吸附菌株的培养方法 |
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CN109652346A CN109652346A (zh) | 2019-04-19 |
CN109652346B true CN109652346B (zh) | 2020-11-10 |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103409346A (zh) * | 2013-07-24 | 2013-11-27 | 徐州工程学院 | 一种重金属抗性果胶杆菌及其应用 |
CN105505821A (zh) * | 2015-12-17 | 2016-04-20 | 福建省微生物研究所 | 假单胞菌(Pseudomonas protegens)S63及其在防治水葫芦中的应用 |
CN106179270A (zh) * | 2016-08-03 | 2016-12-07 | 王维娜 | 一种城市污水中锌污染复合树脂吸附剂及其制备方法 |
CN106222113A (zh) * | 2016-08-24 | 2016-12-14 | 宁波枫叶杰科生物技术有限公司 | 一种快速治理铅锌污染水体的微生物方法 |
CN106916765A (zh) * | 2017-02-21 | 2017-07-04 | 江南大学 | 一种利用微紫青霉菌吸附废水中重金属锌的方法 |
CN108753652A (zh) * | 2018-06-14 | 2018-11-06 | 佛山市碧沃丰生物科技股份有限公司 | 一种降解席藻的方法 |
-
2019
- 2019-02-19 CN CN201910124971.XA patent/CN109652346B/zh not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103409346A (zh) * | 2013-07-24 | 2013-11-27 | 徐州工程学院 | 一种重金属抗性果胶杆菌及其应用 |
CN105505821A (zh) * | 2015-12-17 | 2016-04-20 | 福建省微生物研究所 | 假单胞菌(Pseudomonas protegens)S63及其在防治水葫芦中的应用 |
CN106179270A (zh) * | 2016-08-03 | 2016-12-07 | 王维娜 | 一种城市污水中锌污染复合树脂吸附剂及其制备方法 |
CN106222113A (zh) * | 2016-08-24 | 2016-12-14 | 宁波枫叶杰科生物技术有限公司 | 一种快速治理铅锌污染水体的微生物方法 |
CN106916765A (zh) * | 2017-02-21 | 2017-07-04 | 江南大学 | 一种利用微紫青霉菌吸附废水中重金属锌的方法 |
CN108753652A (zh) * | 2018-06-14 | 2018-11-06 | 佛山市碧沃丰生物科技股份有限公司 | 一种降解席藻的方法 |
Non-Patent Citations (2)
Title |
---|
Bacterial zinc uptake regulator proteins and their regulons;Alevtina Mikhaylina等;《Biochemical Society transactions》;20180820;983–1001页 * |
The effect of zinc limitation on the transcriptome of Pseudomonas protegens Pf5;Chee Kent Lim等;《Environmental Microbiology》;20131231;摘要,第3页右栏最后2段-第4页第1段 * |
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Inventor after: Han Jianjun Inventor after: Wang Yuwen Inventor after: Zhao Chuan Inventor after: Liang Xuejie Inventor before: Wang Yuwen Inventor before: Han Jianjun Inventor before: Zhao Chuan Inventor before: Liang Xuejie |
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