CN109646358B - Compound plant extract with oil control and acne removal effects and application thereof - Google Patents
Compound plant extract with oil control and acne removal effects and application thereof Download PDFInfo
- Publication number
- CN109646358B CN109646358B CN201910106408.XA CN201910106408A CN109646358B CN 109646358 B CN109646358 B CN 109646358B CN 201910106408 A CN201910106408 A CN 201910106408A CN 109646358 B CN109646358 B CN 109646358B
- Authority
- CN
- China
- Prior art keywords
- extract
- compound plant
- parts
- effects
- plant extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a compound plant extract with oil control and acne removal effects, which is prepared by extracting and compounding five plants, namely loquat leaves, phellodendron amurense, artemisia capillaries, mulberry root bark and glycyrrhiza glabra roots. The five plant extracts are combined together, and the effects of reducing excessive secretion of grease, inhibiting growth and reproduction of propionibacterium acnes, preventing release of lipase, reducing inflammation, realizing oil control, bacteriostasis, antioxidation, light mark inhibition of acne and the like are realized through the synergistic effect of the effects. Clinical experiments show that the compound plant extract can effectively reduce skin grease secretion and remove acnes, is safe and non-irritant to skin, is used in cosmetics for controlling and removing acnes, and has good effects of controlling and removing acnes. The compound plant extract is used as an active component, and the compound plant extract is prepared into facial cleanser, smoothing toner, emulsion, cream, essence and facial mask by adopting conventional auxiliary materials and processes for producing skin care products, and has good antioxidant activity, sterilization, skin decoloration and acne mark fading effects.
Description
Technical Field
The invention relates to a compound plant extract with oil control and acne removal effects, which is mainly used for preparing functional skin care products and belongs to the field of daily skin care products.
Background
With the aggravation of the reasons of living pressure, irregular life, food safety, environmental influence and the like, pox is one of the skin problems which afflict many young people. Pockmark is a chronic inflammation of hair follicle sebaceous glands, is common in young men and women in the development period of youth, mainly occurs at shoulder blades of facial muscles and breast muscles to form papules, is called as acne in traditional Chinese medicine, and is called as acne in western medicine. It is often manifested as acne, papules, pustules, nodules, cysts and scars.
Mechanism of pox formation: the increase of androgen can increase the secretion of sebaceous gland, and make the hair follicle and sebaceous gland hyperkeratotic, and the flat dead cells fallen from cuticle can be combined with sebum to produce mixture. If these mixtures are not free to drain out of the skin surface and pool in the hair follicle, a lipid plug is formed. Mature comedones are formed when these abnormal keratinocytes aggregate and the lower funnel swells. Sebum is broken down by bacteria present in the hair follicle to produce fatty acids which stimulate the follicle to cause inflammation, causing the follicle wall to rupture and the follicle contents to enter the dermis, causing an inflammatory response of varying degrees around the follicle.
At present, antibiotics such as metronidazole and the like are used for carrying out antibiosis and antiphlogosis on a plurality of acne-removing products on the market, and the products have certain effects on removing acnes, acnes and the like, but after long-term use, the products can stimulate the skin to cause contact dermatitis which is manifested as erythema, edema, erosion, pruritus and the like, and more serious, the drug resistance to the antibiotics is caused, and health hidden troubles are caused. Therefore, the development of a safe and effective oil-controlling and acne-removing product has very high practical application value and market value.
Disclosure of Invention
The invention aims to provide a compound plant extract with the effects of controlling oil and removing acnes;
the invention also aims to provide a specific application of the compound plant extract with the effects of controlling oil and removing acnes in preparing skin care products.
The traditional Chinese medicine considers that acne is formed mostly due to blood heat, blood deficiency and wind dryness, heat toxin, dampness and the like, so the common method for treating acnes is also from the aspects of removing heat, removing toxin and eliminating dampness. Therefore, the compound plant extract of the invention consists of five plant extracts of loquat leaves, phellodendron amurense, mulberry root bark, artemisia capillaries and glycyrrhiza glabra.
Folium Eriobotryae (leaves of Eriobotrya japonica, Barbados leaves, and Canarium album (lour.) Swingle) is dried leaves of Eriobotrya japonica (Lonicera japonica (Thunb.) Makino of Rosaceae). Mainly produced in Guangdong, Guangxi, Fujian, Yunnan, Zhejiang, Hunan, Jiangsu, Jiangxi, etc. It is bitter in taste, neutral in nature and non-toxic, and enters lung and stomach meridians. Has the functions of clearing lung, eliminating phlegm, relieving cough, lowering adverse qi and arresting vomiting. It can be used for treating cough due to lung heat, and vomiting due to stomach heat. It is widely used clinically because of its lung-heat clearing, phlegm resolving and cough relieving effects. The main components of folium Eriobotryae include triterpenic acids, flavonoids, polyphenols, volatile oils, sesquiterpenes and organic acids. The loquat leaf extract contains abundant flavonoids and triterpenes, has the effects of promoting the generation of ceramide, improving the sebum composition, reducing the secretion of oil and fat, softening the skin and inhibiting 5 alpha-reductase, so that the excessive secretion of the oil and fat can be effectively reduced. Phellodendron amurense, also known as phellodendron amurense, is a plant of Rutaceae (Rutaceae), and phellodendron amurense is an important herb source plant of Chinese medicinal material phellodendron amurense. Mainly produced in the northeast China in the mountainous areas such as the southeast of Daxingan mountains, Xiaoxingan mountains, Changbai mountains, Wandashan mountains, Qianshan mountains and the big provinces of North China. Is cold in nature and bitter in taste, has the effects of clearing heat, purging fire, removing dampness and detoxifying, and is mainly used for treating diseases such as heat dysentery, diarrhea, jaundice, bone steaming, overstrain, atrophy 36484. The cortex phellodendri extract contains berberine and berberine, and has remarkable antibacterial and anti-inflammatory effects. The invention takes loquat leaf and phellodendron amurense extract as monarch drug, and has the main functions of clearing heat and removing toxicity, resisting inflammation and resisting bacteria.
Mulberry root bark, also known as: cortex Mori, and cortex Mori, which are root bark of Morus al-ba L. Sweet in flavor and cold in nature. It enters lung meridian. It has the actions of purging lung, relieving asthma, inducing diuresis to alleviate edema. The cortex Mori extract is rich in flavonoids, coumarins and polysaccharides, and has tranquilizing, analgesic, antiinflammatory and antibacterial effects. The invention takes the mulberry root bark as a ministerial drug and assists a monarch drug to strengthen the effect of purging heat.
Herba Artemisiae Scopariae, and herba Artemisiae Scopariae. Is young seedling of Artemisia capillaris Thunb or Artemisia scoparia Waldst. et kit of Compositae. Shanxi, Shanxi and Anhui are main products. Bitter in taste and slightly cold in nature. It is entered into spleen, stomach, liver and gallbladder meridians. Has the functions of clearing away heat, promoting diuresis, promoting the function of gallbladder and eliminating jaundice. It can be used for treating jaundice, dysuria, damp-heat, eczema, and eczema. The herba Artemisiae Scopariae extract contains volatile oil, and the main ingredients of the oil are beta-pinene, capillin, capillone and folic acid. Herba Artemisiae Scopariae is bitter and cold in flavor, and has effects of clearing heat and promoting diuresis, eliminating jaundice and steaming. The invention takes the artemisia capillaris thunb extract as an adjuvant drug, inhibits various bacteria and fungi and conditions the skin.
Glycyrrhiza glabra is a plant of Glycyrrhiza of Leguminosae. The glabrous licorice root has sweet taste, clears heat, relieves pain and swelling and sore toxicity, and can coordinate the medicines as a guiding medicine; the liquorice has anti-inflammatory and anti-allergic effects; licorice contains liquiritigenin, a hormone-like compound, which helps to balance the hormone content in the female. Research shows that the glycyrrhiza glabra extract mainly contains triterpenoid saponins of glycyrrhizic acid series and flavonoid compounds with various structural types, such as glabridin T, glabridin, glycyrrhetin A, glycyrrhetin B, hemiglycyrrhetin B, formononetin, liquiritigenin and the like, so that the glycyrrhiza glabra extract has antioxidant activity and strong effects of sterilizing, decoloring skin and fading acne marks. The invention takes the glycyrrhiza glabra extract as a messenger drug, coordinates the drugs, and plays the roles of decoloring the skin and fading acne marks.
The five plant extracts are combined, so that the effects of the extracts are synergistic, the growth and reproduction of propionibacterium acnes are inhibited, the release of lipase is prevented, the occurrence of inflammation is reduced by reducing the excessive secretion of grease, and the effects of controlling oil, inhibiting bacteria, resisting oxidation, reducing acne marks, inhibiting acne and the like are realized, so that the aims of controlling oil and removing acne are fulfilled.
The compound plant extract is obtained by low-temperature wall-breaking extraction process. The process acts on the materials by instantaneous high-speed flow (maximum 300 m/s), so that the materials are quickly and strongly collided, plant cells are instantaneously ruptured, low-temperature, quick and full-ingredient extraction is realized, and the oxidation and degradation of thermosensitive ingredients are effectively prevented. Therefore, the color, the aroma and the taste of the obtained extracting solution are consistent with those of the raw materials, the effective components are consistent with those of the plant materials, and the use of the extracting solution is ensured to be consistent with the clinical application theory of the traditional plants.
In order to obtain better effect, the loquat leaf extract, the phellodendron amurense extract, the mulberry root bark extract, the artemisia capillaris extract and the glycyrrhiza glabra root extract are mixed according to the following weight parts: 8-15 parts of loquat leaf extract, 8-15 parts of phellodendron amurense extract, 8-13 parts of mulberry root bark extract, 6-11 parts of artemisia capillaris thumb extract and 5-10 parts of glycyrrhiza glabra root extract.
The preferable mixture ratio is as follows: 9-12 parts of loquat leaf extract, 9-12 parts of phellodendron amurense extract, 7-11 parts of mulberry root bark extract, 6-10 parts of artemisia capillaris thumb extract and 6-8 parts of glycyrrhiza glabra root extract.
The optimal proportion is as follows: 10 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 9 parts of mulberry root bark extract, 8 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract.
In the specific application of the compound plant extract with the effects of controlling oil and removing acnes in preparing the skin care product, the compound plant extract is used as an active component, and conventional auxiliary materials and processes for producing the skin care product are adopted to prepare the facial cleanser, the smoothing toner, the lotion, the cream, the essence and the facial mask. In order to achieve a good oil-control acne-removing effect, the addition amount of the compound plant extract in the skin care product is 1-20 wt%.
Clinical experiments show that the compound plant extract can effectively reduce skin grease secretion and remove acnes, is safe and non-irritant to skin, is used in cosmetics for controlling and removing acnes, and has good effects of controlling and removing acnes.
Detailed Description
The preparation of the compound plant extract and the specific application thereof in the preparation of functional skin care products are further described by the following specific examples.
Example one
1. Preparation of compound plant extract
Sterilizing folium Eriobotryae, cortex Phellodendri, cortex Mori, herba Artemisiae Scopariae, and radix Glycytthizae, adding deionized water as solvent (plant: deionized water =1:40 weight ratio), performing low temperature wall breaking extraction, centrifuging to remove residue, adding glycerol as solvent, performing multi-stage membrane separation and concentration to obtain folium Eriobotryae extract, cortex Phellodendri extract, cortex Mori extract, herba Artemisiae Scopariae extract, and radix Glycytthizae extract. The compound plant extract (I) is prepared from 10 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 9 parts of mulberry root bark extract, 8 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract.
2. Preparation of oil-control acne-removing toner
The formula of the oil-controlling acne-removing toner is shown in table 1;
preparing the oil-controlling acne-removing toner: mixing EDTA-2Na, butanediol and deionized water, heating to 80 ℃, stirring for dissolving, cooling to 45 ℃, adding panthenol, trimethylglycine, preservative and the compound plant extract, mixing and stirring uniformly, and standing to obtain the oil-controlling acne-removing skin-refreshing aquatic products No. 1, No. 2, No. 3 and No. 4.
Example two
1. Preparing a compound plant extract: sterilizing folium Eriobotryae, cortex Phellodendri, cortex Mori, herba Artemisiae Scopariae, and radix Glycytthizae, adding deionized water as solvent (plant: deionized water =1:40 weight ratio), performing low temperature wall breaking extraction, centrifuging to remove residue, adding glycerol as solvent, performing multi-stage membrane separation and concentration to obtain folium Eriobotryae extract, cortex Phellodendri extract, cortex Mori extract, herba Artemisiae Scopariae extract, and radix Glycytthizae extract. The compound plant extract (II) is prepared by 11 parts of loquat leaf extract, 11 parts of phellodendron amurense extract, 9.9 parts of mulberry root bark extract, 8.8 parts of artemisia capillaris extract and 7.7 parts of glycyrrhiza glabra root extract.
2. Preparation of oil-controlling acne-removing cream
The formula of the oil-controlling acne-removing cream is shown in table 2;
preparing the oil-controlling acne-removing cream: respectively heating the oil phase and the water phase to 75 ℃, mixing and homogenizing the two phases for a plurality of minutes, stirring and cooling, cooling to 45 ℃, adding the preservative and the compound plant extract, stirring uniformly, and standing at 35 ℃ to obtain white, fine and uniform emulsion.
EXAMPLE III
1. Preparing a compound plant extract: sterilizing folium Eriobotryae, cortex Phellodendri, cortex Mori, herba Artemisiae Scopariae, and radix Glycytthizae, adding deionized water as solvent (plant: deionized water =1:40 weight ratio), performing low temperature wall breaking extraction, centrifuging to remove residue, adding glycerol as solvent, performing multi-stage membrane separation and concentration to obtain folium Eriobotryae extract, cortex Phellodendri extract, cortex Mori extract, herba Artemisiae Scopariae extract, and radix Glycytthizae extract. The compound plant extract (III) is prepared by 9 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 8 parts of mulberry root bark extract, 7 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract.
2. Preparation of oil-control acne-removing face cream
The oil-control acne-removing facial cream is prepared by taking the compound plant extract (III) as a main active component (the adding amount of the compound plant extract (III)) and adopting conventional auxiliary materials and processes for producing the facial cream.
Example four
1. Preparation of compound plant extract
Sterilizing folium Eriobotryae, cortex Phellodendri, cortex Mori, herba Artemisiae Scopariae, and radix Glycytthizae, adding deionized water as solvent (plant: deionized water =1:40 weight ratio), performing low temperature wall breaking extraction, centrifuging to remove residue, adding glycerol as solvent, performing multi-stage membrane separation and concentration to obtain folium Eriobotryae extract, cortex Phellodendri extract, cortex Mori extract, herba Artemisiae Scopariae extract, and radix Glycytthizae extract. The compound plant extract is prepared by 8 parts of loquat leaf extract, 8 parts of phellodendron amurense extract, 7.2 parts of mulberry root bark extract, 6.4 parts of artemisia capillaris extract and 5.6 parts of glycyrrhiza glabra root extract (the compound plant extract is prepared by the following steps of)。
2. Preparation of oil-control acne-removing mask
Prepared from compound plant extracts () Is the main active component (compound plant extract) (() The addition amount of the oil-controlling acne-removing facial mask is 10 wt.%) and the oil-controlling acne-removing facial mask is prepared by adopting conventional auxiliary materials and processes for producing facial masks.
The oil-controlling and acne-removing efficacy of the compound plant extract is observed through in vitro tests and human body tests.
1. Test for Propionibacterium acnes inhibitory Capacity
Sample preparation: compound plant extract of example 1: () The compound plant extract (III) of example 3, the compound plant extract of example 4。
Preparation of a culture medium: respectively weighing 33 g of lecithin-tween nutrient agar culture medium and 29.3 g of liquid thioglycollate culture medium, adding 1L of ultrapure water, stirring, heating, boiling to completely dissolve, subpackaging, sterilizing at 121 ℃ for 30 min, cooling to 60-70 ℃, and preserving heat in a water bath for later use.
Recovery of propionibacterium acnes and preparation of bacterial suspension: taking propionibacterium acnes freeze-dried powder, fusing an ampoule bottle under an alcohol lamp, adding 1 mL of 0.85% physiological saline, uniformly stirring, inoculating the mixture on 3 flat plates containing a culture medium, and then culturing for 3-4 days under the anaerobic condition at 37 ℃. Scraping the slant colony with an inoculating loop, and properly diluting the bacterial suspension with physiological saline to make the concentration of the bacterial suspension be 106-107 CFU.mL-1. Taking the bacterial suspension under aseptic operation, and uniformly smearing the bacterial suspension on a culture medium by using a coating rod. Then, the Oxford cup is lightly placed on the surface of the solidified culture medium by using sterile forceps, and the Oxford cup (the outer diameter is 8 mm) is lightly pressed to ensure good contact. Adding 200 μ L of compound plant extract liquid into an Oxford cup, culturing in an incubator at 37 deg.C for 48 h, taking out, and measuring the diameter of effective zone of inhibition of the test solution in each culture dish. Each set of experiments was repeated twice, and samples were taken twice each time for testing.
Antibacterial effect judgment standard: the high sensitivity is that the diameter of the bacteriostatic zone is more than 20 mm; the diameter of the inhibition zone is 10-20 mm, and the medium sensitivity is determined; the diameter of the inhibition zone is less than 10 mm, and the drug resistance is achieved.
The bacteriostatic effect is as follows: see table 3:
as can be seen from table 3, the bacteriostatic effect of the compound plant extract is from strong to weak: i > III ≈. Therefore, the compound plant extract is prepared according to 10 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 9 parts of mulberry root bark extract, 8 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract, and the antibacterial effect is optimal. If not in this ratioIn the range of the examples, even if the added plant ingredients are more, the bacteriostatic effect is equivalent to that of the added plant ingredients with less addition.
2. Assay for inhibition of 5 alpha-reductase
Sample preparation: compound plant extract of example 1: () The compound plant extract (III) of example 3, the compound plant extract of example 4。
The test method comprises the following steps: simulating a 5 alpha-reductase catalytic reaction system in an animal body, and establishing the reaction system with the total volume of 100 mu L. The ratio of the testosterone to the 5 alpha-reductase to the phosphate buffer solution to the reduced coenzyme II to the medicament to be screened is 2:3:29:15: 1. The primary function of 5 α -reductase is to reduce testosterone to Dihydrotestosterone (DHT), which may cause acne in the face when the DHT level in the skin of the human body is too high.
Blank control group: adding testosterone T, 5 alpha-reductase, phosphate buffer solution and reduced coenzyme II into the reaction tube in sequence, uniformly mixing, determining absorbance A340 nm value at 0 min, incubating at 37 deg.C, determining absorbance A340 nm value at 60 min, deducting blank reduced background value of reduced coenzyme II, and obtaining absorbance reduced value (delta A)0). Group to be tested: adding testosterone T, 5 alpha-reductase, compound plant extract, phosphate buffer solution, and reduced coenzyme II into reaction tube in sequence, mixing, measuring absorbance A340 nm value at 0 min, incubating at 37 deg.C, measuring absorbance A340 nm value at 60 min, deducting blank reduced background value of reduced coenzyme II to obtain absorbance reduced value (Δ A)n). Comparison of Δ A0And Δ AnThe value of the value is determined whether the activity of the enzyme is inhibited or not, and the formula I% = (Delta A)0-ΔAn)/ΔA0X 100%, the magnitude of the inhibition rate was preliminarily calculated. The results are shown in Table 4:
as can be seen from table 4, the tests for the compound plant extract to inhibit 5 α -reductase were, in order from strong to weak: i > III ≈. Therefore, the compound plant extract is prepared according to 10 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 9 parts of mulberry root bark extract, 8 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract, and has the best effect of inhibiting 5 alpha-reductase. If the amount is not within the above range, the effect of inhibiting 5. alpha. -reductase is equivalent to that of a smaller amount of the plant component even if the plant component is added in a larger amount.
3. Oil control Effect test
Sample preparation: oil-controlling, acne-removing toner # 3 prepared in example 1; and (3) comparison: the compound plant extract (I) of the oil-controlling, acne-removing toner 3# is removed.
The test method comprises the following steps: volunteers 30, subjects were randomized into 3 groups of 10. The subjects sit still for 30 minutes in a laboratory with the temperature of 22 +/-3 ℃ and the relative humidity of 50 +/-5 percent, the skin oil content of the left forehead and the right forehead is respectively tested by using an oil tester Sebumeter SM815, the data is recorded as D0, the samples and the control samples are distributed to each subject, the subjects are guided to carry out half-face comparison and trial, the two samples are respectively smeared on the left half-face or the right half-face, 0.5g of the sample is smeared once in the morning and at the evening every day for 28 days, the skin oil content is respectively tested in a constant temperature laboratory on the 14 th day and the 28 th day, and the data is recorded as D14 and D28. Respectively calculating the oil content change rate of D14 and D28 according to the following formula:
the rate of change in fat content { (fat content after use sample-fat content before use sample)/fat content before use sample } × 100%
The results in table 5 show that, when the experimental sample added with the compound plant extract prepared in example 1 of the present invention is used, the skin oil content is significantly reduced in 14 days, and the effect is more significant after the experimental sample is continuously used for 28 days, while the oil control effect is significantly worse when the experimental sample is continuously used without the compound plant extract prepared in example 1.
4. Improvements in acne, pimples and pustules
Sample preparation: oil-controlling acne-removing cream # 3 prepared in example 2. The face pack is tried by 30 people for 28 days, the age is 16-30 years old, 30 people are oily mixed skin, and the face has acnes, papules and pustules with different degrees.
The using method comprises the following steps: the product is applied topically for 2 times every day, and the dosage is uniform for face application.
After 28 days of use, there was a significant improvement in skin condition, with 26 (86%) of the subjects experiencing marked oil development, and with 23 (76%) of the subjects experiencing marked acne, papules and pustules, with 18 (60%) of the subjects experiencing the acne marks and also being subdued.
Claims (4)
1. A compound plant extract with effects of controlling oil and removing acne is prepared from folium Eriobotryae extract, cortex Phellodendri extract, cortex Mori extract, herba Artemisiae Scopariae extract, and radix Glycytthizae extract; the plant extracts are prepared from the following components in parts by weight: 10 parts of loquat leaf extract, 10 parts of phellodendron amurense extract, 9 parts of mulberry root bark extract, 8 parts of artemisia capillaris extract and 7 parts of glycyrrhiza glabra root extract; the plant extracts are obtained by adopting a low-temperature wall-breaking extraction process.
2. The use of the compound plant extract with the effects of controlling oil and removing acne as claimed in claim 1 in the preparation of skin care products.
3. The application of the compound plant extract with the effects of controlling oil and removing acnes in preparing the skin care product as claimed in claim 2, is characterized in that: the skin softening lotion, the emulsion, the cream, the essence and the facial mask are prepared by taking the compound plant extract as an active component and adopting conventional auxiliary materials and processes for producing skin care products.
4. The application of the compound plant extract with the effects of controlling oil and removing acnes in preparing the skin care product as claimed in claim 3, is characterized in that: the addition amount of the compound plant extract in the skin care product is 1-20 wt% in terms of mass fraction.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910106408.XA CN109646358B (en) | 2019-02-02 | 2019-02-02 | Compound plant extract with oil control and acne removal effects and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910106408.XA CN109646358B (en) | 2019-02-02 | 2019-02-02 | Compound plant extract with oil control and acne removal effects and application thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109646358A CN109646358A (en) | 2019-04-19 |
CN109646358B true CN109646358B (en) | 2021-08-10 |
Family
ID=66122149
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910106408.XA Active CN109646358B (en) | 2019-02-02 | 2019-02-02 | Compound plant extract with oil control and acne removal effects and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109646358B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111329807A (en) * | 2020-04-02 | 2020-06-26 | 广州市逸轩精细化工有限公司 | Acne removing cream and preparation method thereof |
AU2021320960A1 (en) * | 2020-08-07 | 2023-03-09 | Kikkoman Corporation | Food/beverage article |
CN112006960A (en) * | 2020-09-15 | 2020-12-01 | 广州莱约生物科技有限公司 | Acne-removing and oil-controlling composition and preparation method and application thereof |
CN112168949B (en) * | 2020-10-14 | 2023-01-24 | 北京斯利安药业有限公司 | Health product for adolescent women |
CN115252487A (en) * | 2022-04-28 | 2022-11-01 | 上海煌萱实业有限公司 | Sports type oil-controlling, soothing and moisturizing plant care solution and preparation method thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1342474A (en) * | 2000-09-08 | 2002-04-03 | 俞宝明 | Functional additive for cosmetics |
CN1840115A (en) * | 2006-01-10 | 2006-10-04 | 孙小五 | Acne-treating medicinal soap and preparation method thereof |
CN101869618A (en) * | 2010-06-03 | 2010-10-27 | 宋丽峰 | Chinese medicinal acne-removal mixture for treating acne |
CN105796413A (en) * | 2016-03-17 | 2016-07-27 | 陆娟 | Traditional Chinese medicine facial mask and preparing method thereof |
-
2019
- 2019-02-02 CN CN201910106408.XA patent/CN109646358B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1342474A (en) * | 2000-09-08 | 2002-04-03 | 俞宝明 | Functional additive for cosmetics |
CN1840115A (en) * | 2006-01-10 | 2006-10-04 | 孙小五 | Acne-treating medicinal soap and preparation method thereof |
CN101869618A (en) * | 2010-06-03 | 2010-10-27 | 宋丽峰 | Chinese medicinal acne-removal mixture for treating acne |
CN105796413A (en) * | 2016-03-17 | 2016-07-27 | 陆娟 | Traditional Chinese medicine facial mask and preparing method thereof |
Non-Patent Citations (1)
Title |
---|
复合植物控油祛痘因子;富煜陇 Forulong;《https://mp.weixin.qq.com/s/w-aVBODjZi1yakfY3nUMyQ》;20180619;第1-3页 * |
Also Published As
Publication number | Publication date |
---|---|
CN109646358A (en) | 2019-04-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109646358B (en) | Compound plant extract with oil control and acne removal effects and application thereof | |
KR101310970B1 (en) | Oriental medicine extract for prevention of alopecia and hair article composition | |
KR100879032B1 (en) | Natural antibacterial extracts and cosmetic composition comprising the extracts | |
CN105434307B (en) | A kind of Whelk-eliminating paste containing Chinese herbal medicine extract and preparation method thereof | |
KR101991830B1 (en) | Composition for cosmetics comprising glycyrrhiza radix extract and centella asiatica extract using mineral-rich hot spring water as extraction solvent | |
CN113274331B (en) | Acne-removing anti-inflammatory composition and preparation method and application thereof | |
CN108904376B (en) | Traditional Chinese medicine compound fermentation liquor for cosmetics with oil control and acne removal effects and preparation and application thereof | |
CN114028295B (en) | Antibacterial itching-relieving red-fading acne-removing repairing composition as well as preparation method and application thereof | |
CN112569152B (en) | Oil-control anti-inflammatory plant combined extract and preparation method and application thereof | |
CN110151639A (en) | A kind of acne eliminating cream and preparation method thereof containing oldenlandia diffusa | |
CN110403880A (en) | A kind of herb fermenting object and its hand lotion of preparation | |
CN110251448A (en) | A kind of cosmetic compositions containing propolis extract | |
CN103041042B (en) | Tibetan medicine composition and application of composition in anti-off hair products | |
CN114010553A (en) | Composition for controlling oil and refining pores and preparation and application thereof | |
CN105168385A (en) | Antibacterial and itching-relieving medicine composition and preparing method thereof | |
CN105342916B (en) | A kind of composition of plant extracts and its preparation method and application | |
CN110585044A (en) | Compound traditional Chinese medicine composition containing dendrobium stem and preparation method thereof | |
CN113274455A (en) | Traditional Chinese medicine composition and application thereof | |
KR20140021315A (en) | Cosmetic compositon for skin-irritation alleviation with inula britannica var. chinensis, cynanchi radix and lysimachia foenum-graecum hance | |
CN110101648B (en) | Beauty-care concealer air cushion BB cream | |
KR100747415B1 (en) | Composition for improving mastitis comprising herb extracts | |
CN105412192A (en) | Extracting method of folium artemisiae argyi mite-removing cream and folium artemisiae argyi volatile oil | |
KR20160118417A (en) | Manufacturing Method and Composion for Viper Venom Liquid Mixture | |
CN108210433A (en) | Cardamom skin care compositions and its application with oil-control and/or acne-removing | |
KR101770413B1 (en) | A composition for the prevention or treatment of edema or dermatitis containing oriental medicine herbs oil extract as an active ingredient |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 510425 lianbian international 818, 361 and 363 Jianpeng Road, Helong street, Baiyun District, Guangzhou City, Guangdong Province (self declaration) Applicant after: Guangzhou fuyulong Biotechnology Co.,Ltd. Address before: 510600 room 3a08, No. 22, No. 1 Industrial Zone, Heguang Road, Tianhe District, Guangzhou City, Guangdong Province Applicant before: GUANGZHOU FORULONG CHEMICAL TECHNOLOGY Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant |