CN109576150A - Membrane DNA chip hybridization instrument - Google Patents

Membrane DNA chip hybridization instrument Download PDF

Info

Publication number
CN109576150A
CN109576150A CN201811652671.0A CN201811652671A CN109576150A CN 109576150 A CN109576150 A CN 109576150A CN 201811652671 A CN201811652671 A CN 201811652671A CN 109576150 A CN109576150 A CN 109576150A
Authority
CN
China
Prior art keywords
liquid
peristaltic pump
connecting line
dna chip
connect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811652671.0A
Other languages
Chinese (zh)
Inventor
江明锋
黄莉
彭丽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Huahan Trio Biotechnology Co Ltd
Southwest Minzu University
Original Assignee
Sichuan Huahan Trio Biotechnology Co Ltd
Southwest Minzu University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Huahan Trio Biotechnology Co Ltd, Southwest Minzu University filed Critical Sichuan Huahan Trio Biotechnology Co Ltd
Priority to CN201811652671.0A priority Critical patent/CN109576150A/en
Publication of CN109576150A publication Critical patent/CN109576150A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses membrane DNA chip hybridization instruments, are related to hybridization instrument technical field.Membrane DNA chip hybridization instrument provided by the invention includes apparatus body, sampling assemble, draining component, connecting line and reaction component, and sampling assemble, draining component, connecting line and reaction component are arranged at apparatus body.Sampling assemble includes sampling probe and peristaltic pump, and sampling probe is connect with peristaltic pump, and peristaltic pump is used to sample when opening by sampling probe.Draining component includes mounting disc, inlet tube and multiple liquid-controlling valves, inlet tube and multiple liquid-controlling valves are arranged in mounting disc, the connection of the efferent duct of inlet tube and peristaltic pump, multiple liquid-controlling valves are connect by connecting line with reaction component, and liquid-controlling valve is used to that liquid to be delivered to reaction component by connecting line when opening.Membrane DNA chip hybridization instrument provided by the invention has the characteristics that structure is simply and easy to use, can implement gene film chip detection method.

Description

Membrane DNA chip hybridization instrument
Technical field
The present invention relates to hybridization instrument technical fields, in particular to membrane DNA chip hybridization instrument.
Background technique
Traditional micro-array biochip analysis system, it is several to generally comprise chip hybridization, cleaning, dyeing and detection etc. Link, these links are had been manually done by operator, are not only taken time and effort, but also manual operation can bring experimental error, Polluted possibility.There is also membrane DNA chip hybridization instruments in the prior art, but the setting between each module is complex, makes It is inconvenient with existing with operation.
Summary of the invention
The purpose of the present invention is to provide a kind of membrane DNA chip hybridization instrument, have the characteristics that structure is simply and easy to use, It can implement gene film chip detection method.
The present invention provides a kind of technical solution about membrane DNA chip hybridization instrument:
A kind of membrane DNA chip hybridization instrument, including apparatus body, sampling assemble, draining component, connecting line and reaction component, institute It states sampling assemble, the draining component, the connecting line and the reaction component and is arranged at the apparatus body;It is described to take Sample component includes sampling probe and peristaltic pump, and the sampling probe is connect with the peristaltic pump, and the peristaltic pump is used for logical when opening Cross the sampling probe sampling;The draining component includes mounting disc, inlet tube and multiple liquid-controlling valves, the inlet tube and multiple institutes It states liquid-controlling valve to be arranged in the mounting disc, the inlet tube is connect with the efferent duct of the peristaltic pump, multiple drainings Valve is connect by the connecting line with the reaction component, and the liquid-controlling valve is used to that liquid to be passed through the connection when opening Pipeline transport is to the reaction component.
Optionally, the connecting line includes fixing piece and multiple liquid lines for being arranged on the fixing piece, the liquid Body pipe is set on the apparatus body by the fixing piece, the both ends of the liquid line respectively with the liquid-controlling valve and described Reaction component connection.
Optionally, the connecting line further includes overflow passage, and liquid tank, the overflow pipe are provided on the fixing piece The both ends on road are connect with the liquid tank and the reaction component respectively;The inclined bottom surface of the liquid tank is arranged and in lower end It is provided with liquid outlet.
Optionally, the sampling assemble further includes driving equipment, and the sampling probe and the driving equipment are sequentially connected, institute Driving equipment is stated for driving the sampling needle movement, so that the sampling probe is sampled when the peristaltic pump is opened.
Optionally, the driving equipment is cylinder, hydraulic cylinder, screw pair or linear motor, and the driving is set It is standby that its direction of motion is provided with guide rail.
Optionally, the reaction component includes card holding structure and chip card box, the chip card box have chamber and with institute The opening of chamber is stated, the connecting line and the opening connect, and the card holding structure is set to the apparatus body, and The chip card box can be fastened in the card holding structure;It is described when the chip card box is fastened on the card holding structure Connecting line and the opening connect, and the liquid is delivered to the chip card box.
Optionally, the chip card box includes cartridge ontology and box cover, and the cartridge ontology has the chamber and described Opening, the cartridge ontology and the box cover are tightly connected, to close the chamber.
Optionally, the box cover is provided with interconnecting piece, and the interconnecting piece and the cartridge ontology are rotatablely connected.
Optionally, the membrane DNA chip hybridization instrument further includes waste-solution line, and the waste-solution line is connect with the peristaltic pump, institute Peristaltic pump is stated to be also used to the liquid in the reaction component being delivered to the waste-solution line.
Technical solution the present invention also provides another kind about membrane DNA chip hybridization instrument:
A kind of membrane DNA chip hybridization instrument, including controller, apparatus body, sampling assemble, draining component, connecting line and reaction Component, the sampling assemble, the draining component, the connecting line and the reaction component are arranged at the equipment sheet Body;The sampling assemble includes sampling probe and peristaltic pump, and the sampling probe is connect with the peristaltic pump, and the peristaltic pump is used for It is sampled when unlatching by the sampling probe, the peristaltic pump is electrically connected with the controller, and the controller is described for controlling The working condition of peristaltic pump;The draining component includes mounting disc, inlet tube and multiple liquid-controlling valves, the inlet tube and multiple institutes It states liquid-controlling valve to be arranged in the mounting disc, the inlet tube is connect with the efferent duct of the peristaltic pump, multiple drainings Valve is connect by the connecting line with the reaction component, and the liquid-controlling valve is used to that liquid to be passed through the connection when opening Pipeline transport to the reaction component, the liquid-controlling valve is electrically connected with the controller, and the controller is also used to alternatively open Open the liquid-controlling valve.
Compared with prior art, the beneficial effect of membrane DNA chip hybridization instrument provided by the invention is:
Sampling assemble is for sampling, and wherein sampling probe is used for the sucting reaction liquid when peristaltic pump is opened.Draining component is used for Control liquid flow path, wherein reaction solution is delivered to inlet tube by the connection of the efferent duct of inlet tube and peristaltic pump;Multiple drainings Valve corresponds respectively to connecting line, and reaction solution is delivered to reaction component by connecting line when liquid-controlling valve is opened.Connecting line It is corresponding with multiple liquid-controlling valves and multiple reaction components, reaction solution is delivered to corresponding reaction component when liquid-controlling valve is opened. Reaction component is for carrying out gene film chip detection.In addition, upon completion of the assays, can also be inverted by peristaltic pump, will react Waste liquid discharge afterwards.Equipment can also be cleaned by peristaltic pump and connecting line etc..Film provided in an embodiment of the present invention Chip hybridization instrument has the characteristics that structure is simply and easy to use, can implement gene film chip detection method.Meanwhile the present invention The membrane DNA chip hybridization instrument that embodiment provides can automatically realize gene film chip detection method.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached Figure is briefly described.It should be appreciated that the following drawings illustrates only certain embodiments of the present invention, therefore it is not construed as pair The restriction of range.It for those of ordinary skill in the art, without creative efforts, can also be according to this A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the structural schematic block diagram for the membrane DNA chip hybridization instrument that the embodiment of the present invention provides;
Fig. 2 is the structural schematic diagram for the sampling assemble that the embodiment of the present invention provides;
Fig. 3 is the structural schematic diagram for the draining component that the embodiment of the present invention provides;
Fig. 4 is the structural schematic diagram for the liquid line that the embodiment of the present invention provides;
Fig. 5 is the structural schematic diagram for the fixing piece that the embodiment of the present invention provides;
Fig. 6 is the structural schematic diagram for the cartridge ontology that the embodiment of the present invention provides.
Icon: 10- membrane DNA chip hybridization instrument;100- apparatus body;200- sampling assemble;210- sampling probe;220- peristaltic pump; 230- driving equipment;300- draining component;310- mounting disc;320- inlet tube;330- liquid-controlling valve;400- connecting line;410- Fixing piece;412- liquid tank;420- liquid line;430- overflow passage;500- reaction component;510- chip card box;511- cartridge Ontology;512- box cover;513- chamber;514- opening;515- interconnecting piece.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described.Obviously, described embodiment is A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present invention, it is to be understood that, term " on ", "lower", "inner", "outside", "left", "right" etc. indicate Orientation or positional relationship be based on the orientation or positional relationship shown in the drawings or the invention product using when usually put Orientation or positional relationship or the orientation or positional relationship that usually understands of those skilled in the art, be merely for convenience of retouching It states the present invention and simplifies description, rather than the equipment of indication or suggestion meaning or element must have a particular orientation, with specific Orientation construction and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " etc. are only used for distinguishing description, it is not understood to indicate or imply relatively important Property.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, " setting ", " even Connect " etc. terms shall be understood in a broad sense, for example, " connection " may be a fixed connection, may be a detachable connection, or integrally connect It connects;It can be mechanical connection, be also possible to be electrically connected;It can be and be directly connected to, can also be indirectly connected with by intermediary, it can To be the connection inside two elements.For the ordinary skill in the art, can understand as the case may be above-mentioned The concrete meaning of term in the present invention.
With reference to the accompanying drawing, detailed description of the preferred embodiments.
Please refer to Fig. 1 to Fig. 6, present embodiments provide a kind of membrane DNA chip hybridization instrument 10, with structure simply and Feature easy to use can implement gene film chip detection method.
It should be noted that gene film chip detection method for animals and plants breeding chip, animal derived materials, cause a disease it is micro- Detection such as biological, plant-derived, transgenosis and anaphylactogen composition etc..Functional department can be improved to animals and plants in the introducing of this method The related scientific research such as breeding, food-borne plant, animal, pathogenic microorganisms are horizontal, have to the detectability for improving laboratory certain Meaning.Membrane DNA chip hybridization instrument 10 provided in this embodiment can be used for implementing gene film chip detection method.
Referring to Fig. 1, membrane DNA chip hybridization instrument 10 provided in this embodiment includes apparatus body 100, sampling assemble 200, control Liquid component 300, connecting line 400 and reaction component 500, sampling assemble 200, draining component 300, connecting line 400 and reaction Component 500 is arranged at apparatus body 100.
Optionally, sampling assemble 200 further includes driving equipment 230, and sampling probe 210 and driving equipment 230 are sequentially connected, drive Dynamic equipment 230 is for driving sampling probe 210 to move, so that sampling probe 210 is sampled when peristaltic pump 220 is opened.
It is understood that driving equipment 230 can be cylinder, hydraulic cylinder, screw pair or linear motor, and drive Guide rail can be set in its direction of motion in dynamic equipment 230, to motion guide.
Referring to Fig. 2, sampling assemble 200 includes sampling probe 210 and peristaltic pump 220, sampling probe 210 and peristaltic pump 220 connect It connects, peristaltic pump 220 is used to sample when opening by sampling probe 210.
Referring to Fig. 3, draining component 300 includes mounting disc 310, inlet tube 320 and multiple liquid-controlling valves 330, inlet tube 320 It is arranged in mounting disc 310 with multiple liquid-controlling valves 330, inlet tube 320 is connect with the efferent duct of peristaltic pump 220, multiple drainings Valve 330 is connect by connecting line 400 with reaction component 500, and liquid-controlling valve 330 is used to that liquid to be passed through connecting line when opening 400 are delivered to reaction component 500.
It is understood that sampling assemble 200, for sampling, wherein sampling probe 210 is used to inhale when peristaltic pump 220 is opened Enter reaction solution.Draining component 300 is for controlling liquid flow path, wherein and inlet tube 320 is connect with the efferent duct of peristaltic pump 220, with Reaction solution is delivered to inlet tube 320;Multiple liquid-controlling valves 330 correspond respectively to connecting line 400, will when liquid-controlling valve 330 is opened Reaction solution is delivered to reaction component 500 by connecting line 400.Connecting line 400 and multiple liquid-controlling valves 330 and multiple react group Part 500 is corresponding, reaction solution is delivered to corresponding reaction component 500 when liquid-controlling valve 330 is opened.Reaction component 500 is used for Carry out gene film chip detection.In addition, upon completion of the assays, can also be inverted by peristaltic pump 220, the waste liquid after reaction is arranged Out.Equipment can also be cleaned by peristaltic pump 220 and connecting line 400 etc..Membrane DNA chip hybridization provided in this embodiment Instrument 10 has the characteristics that structure is simply and easy to use, can implement gene film chip detection method.Meanwhile the present embodiment provides Membrane DNA chip hybridization instrument 10 can automatically realize gene film chip detection method.
Referring to Fig. 4, optionally, connecting line 400 includes fixing piece 410 and the multiple liquid being arranged on fixing piece 410 Body pipe 420, liquid line 420 are set on apparatus body 100 by fixing piece 410, the both ends of liquid line 420 respectively with liquid-controlling valve 330 and reaction component 500 connect.
It should be noted that the quantity of liquid line 420 is identical as the quantity of liquid-controlling valve 330, and the number with reaction component 500 Amount is consistent.Liquid-controlling valve 330, liquid line 420 and reaction component 500 are correspondingly arranged, and reaction solution is delivered to reaction component 500.
Referring to Fig. 5, optionally, connecting line 400 further includes overflow passage 430, liquid tank is provided on fixing piece 410 412, the both ends of overflow passage 430 are connect with liquid tank 412 and reaction component 500 respectively;The inclined bottom surface of liquid tank 412 is arranged And lower end is provided with liquid outlet.
It should be noted that the quantity of overflow passage 430 is identical as the quantity of reaction component 500, fixing piece 410 is on the one hand It is capable of fixing liquid line 420 and overflow passage 430, on the other hand, when reaction solution overflows in reaction component 500, can be passed through Overflow pipe is delivered to liquid tank 412.The inclined bottom surface setting of liquid tank 412 can be convenient for liquid outlet of the liquid from lower one end Place's outflow.
Referring to Fig. 6, optionally, reaction component 500 includes card holding structure and chip card box 510, chip card box 510 has Chamber 513 and the opening 514 being connected to chamber 513, connecting line 400 are connect with opening 514, and card holding structure is set to equipment sheet Body 100, and chip card box 510 can be fastened in card holding structure;When chip card box 510 is fastened on card holding structure, connecting tube Road 400 is connect with opening 514, and liquid is delivered to chip card box 510.
Optionally, in the present embodiment, chip card box 510 includes cartridge ontology 511 and box cover 512, and cartridge ontology 511 has There are chamber 513 and opening 514, cartridge ontology 511 and box cover 512 are tightly connected, with closed chamber 513.
Further, in the present embodiment, box cover 512 is provided with interconnecting piece 515, interconnecting piece 515 with 511 turns of cartridge ontology Dynamic connection.
Optionally, membrane DNA chip hybridization instrument 10 can also include waste-solution line (not shown), and waste-solution line and peristaltic pump 220 connect It connects, peristaltic pump 220 is also used to the liquid in reaction component 500 being delivered to waste-solution line.When after the reaction was completed, peristaltic pump 220 Liquid in chip card box 510 is delivered to waste-solution line by connecting line 400 by reversion.Waste-solution line, which can connect, to be used for Collect the containers such as the waste liquid pool of waste liquid.
Fig. 1 to Fig. 6 is please referred to, the present embodiment also provides another membrane DNA chip hybridization instrument 10 comprising controller is set Standby ontology 100, sampling assemble 200, draining component 300, connecting line 400 and reaction component 500, sampling assemble 200, draining group Part 300, connecting line 400 and reaction component 500 are arranged at apparatus body 100.Sampling assemble 200 includes 210 He of sampling probe Peristaltic pump 220, sampling probe 210 are connect with peristaltic pump 220, and peristaltic pump 220 is used to sample when opening by sampling probe 210, compacted Dynamic pump 220 is electrically connected with the controller, and controller is used to control the working condition of peristaltic pump 220.Draining component 300 includes mounting disc 310, inlet tube 320 and multiple liquid-controlling valves 330, inlet tube 320 and multiple liquid-controlling valves 330 are arranged in mounting disc 310, feed liquor Pipe 320 is connect with the efferent duct of peristaltic pump 220, and multiple liquid-controlling valves 330 are connect by connecting line 400 with reaction component 500, is controlled Liquid valve 330 is used to that liquid to be delivered to reaction component 500, liquid-controlling valve 330 and controller electricity by connecting line 400 when opening Connection, controller are also used to alternatively open liquid-controlling valve 330.
Membrane DNA chip hybridization instrument 10 provided in this embodiment the utility model has the advantages that sampling assemble 200 for sampling, wherein sampling probe 210 for the sucting reaction liquid when peristaltic pump 220 is opened.Draining component 300 is for controlling liquid flow path, wherein inlet tube 320 It is connect with the efferent duct of peristaltic pump 220, reaction solution is delivered to inlet tube 320;Multiple liquid-controlling valves 330 correspond respectively to connect Reaction solution is delivered to reaction component 500 by connecting line 400 when liquid-controlling valve 330 is opened by pipeline 400.Connecting line 400 with Multiple liquid-controlling valves 330 and multiple reaction components 500 are corresponding, corresponding anti-to be delivered to reaction solution when liquid-controlling valve 330 is opened Answer component 500.Reaction component 500 is for carrying out gene film chip detection.In addition, upon completion of the assays, wriggling can also be passed through 220 reversion of pump, the waste liquid after reaction is discharged.Equipment can also be carried out by peristaltic pump 220 and connecting line 400 etc. clear It washes.Membrane DNA chip hybridization instrument 10 provided in this embodiment has the characteristics that structure is simply and easy to use, can implement gene film core Chip detection method.Meanwhile membrane DNA chip hybridization instrument 10 provided in this embodiment can automatically realize gene film chip detection side Method.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (10)

1. a kind of membrane DNA chip hybridization instrument, which is characterized in that including apparatus body, sampling assemble, draining component, connecting line and anti- Component is answered, the sampling assemble, the draining component, the connecting line and the reaction component are arranged at the equipment sheet Body;
The sampling assemble includes sampling probe and peristaltic pump, and the sampling probe is connect with the peristaltic pump, and the peristaltic pump is used for It is sampled when opening by the sampling probe;
The draining component includes mounting disc, inlet tube and multiple liquid-controlling valves, and the inlet tube and multiple liquid-controlling valves are all provided with It sets in the mounting disc, the inlet tube is connect with the efferent duct of the peristaltic pump, and multiple liquid-controlling valves pass through the company Adapter tube road is connect with the reaction component, and the liquid-controlling valve is used to that liquid to be delivered to institute by the connecting line when opening State reaction component.
2. membrane DNA chip hybridization instrument according to claim 1, which is characterized in that the connecting line includes fixing piece and setting Multiple liquid lines on the fixing piece, the liquid line is set on the apparatus body by the fixing piece, described The both ends of liquid line are connect with the liquid-controlling valve and the reaction component respectively.
3. membrane DNA chip hybridization instrument according to claim 2, which is characterized in that the connecting line further includes overflow passage, Liquid tank is provided on the fixing piece, the both ends of the overflow passage connect with the liquid tank and the reaction component respectively It connects;The inclined bottom surface of the liquid tank is arranged and lower end is provided with liquid outlet.
4. membrane DNA chip hybridization instrument according to claim 1, which is characterized in that the sampling assemble further includes driving equipment, The sampling probe and the driving equipment are sequentially connected, and the driving equipment is for driving the sampling needle movement, so that described Sampling probe is sampled when the peristaltic pump is opened.
5. membrane DNA chip hybridization instrument according to claim 4, which is characterized in that the driving equipment is cylinder, hydraulic cylinder, silk Thick stick pair of nut or linear motor, and the driving equipment is provided with guide rail along its direction of motion.
6. membrane DNA chip hybridization instrument according to claim 1, which is characterized in that the reaction component includes card holding structure and core Piece cartridge, the chip card box have chamber and the opening with the chamber, and the connecting line and the opening connect, The card holding structure is set to the apparatus body, and the chip card box can be fastened in the card holding structure;In the core When piece cartridge is fastened on the card holding structure, the connecting line and the opening are connected, and the liquid is delivered to institute State chip card box.
7. membrane DNA chip hybridization instrument according to claim 6, which is characterized in that the chip card box includes cartridge ontology and box Lid, the cartridge ontology have the chamber and the opening, and the cartridge ontology and the box cover are tightly connected, to close State chamber.
8. membrane DNA chip hybridization instrument according to claim 7, which is characterized in that the box cover is provided with interconnecting piece, the company Socket part and the cartridge ontology are rotatablely connected.
9. membrane DNA chip hybridization instrument according to claim 1, which is characterized in that the membrane DNA chip hybridization instrument further includes sewer pipe Road, the waste-solution line are connect with the peristaltic pump, and the peristaltic pump is also used to for the liquid in the reaction component being delivered to The waste-solution line.
10. a kind of membrane DNA chip hybridization instrument, which is characterized in that including controller, apparatus body, sampling assemble, draining component, connection Pipeline and reaction component, the sampling assemble, the draining component, the connecting line and the reaction component are arranged at institute State apparatus body;
The sampling assemble includes sampling probe and peristaltic pump, and the sampling probe is connect with the peristaltic pump, and the peristaltic pump is used for It is sampled when opening by the sampling probe, the peristaltic pump is electrically connected with the controller, and the controller is for controlling institute State the working condition of peristaltic pump;
The draining component includes mounting disc, inlet tube and multiple liquid-controlling valves, and the inlet tube and multiple liquid-controlling valves are all provided with It sets in the mounting disc, the inlet tube is connect with the efferent duct of the peristaltic pump, and multiple liquid-controlling valves pass through the company Adapter tube road is connect with the reaction component, and the liquid-controlling valve is used to that liquid to be delivered to institute by the connecting line when opening Reaction component is stated, the liquid-controlling valve is electrically connected with the controller, and the controller is also used to alternatively open the liquid-controlling valve.
CN201811652671.0A 2018-12-29 2018-12-29 Membrane DNA chip hybridization instrument Pending CN109576150A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811652671.0A CN109576150A (en) 2018-12-29 2018-12-29 Membrane DNA chip hybridization instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811652671.0A CN109576150A (en) 2018-12-29 2018-12-29 Membrane DNA chip hybridization instrument

Publications (1)

Publication Number Publication Date
CN109576150A true CN109576150A (en) 2019-04-05

Family

ID=65915691

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811652671.0A Pending CN109576150A (en) 2018-12-29 2018-12-29 Membrane DNA chip hybridization instrument

Country Status (1)

Country Link
CN (1) CN109576150A (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102134552A (en) * 2010-12-31 2011-07-27 福建泰普生物科学有限公司 Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same
CN103949292A (en) * 2014-04-04 2014-07-30 北京贝泰科技有限公司 Multi-target microarray chip full-automatic hybrid cleaning dyeing system
CN104946510A (en) * 2014-03-31 2015-09-30 博奥生物集团有限公司 Nucleic acid amplification/microarray detection integrated microfluidic device
CN107299054A (en) * 2017-01-12 2017-10-27 武汉菲思特生物科技有限公司 The control system and control method of DNA sequencing device
CN207498376U (en) * 2017-09-26 2018-06-15 南京岚煜生物科技有限公司 The micro-fluidic nucleic acid detection chip of more flux of active control flow path
CN108641902A (en) * 2018-06-08 2018-10-12 四川华汉三创生物科技有限公司 Hybridization instrument and molecular detection system
CN108642142A (en) * 2018-06-08 2018-10-12 四川华汉三创生物科技有限公司 Cartridge component and hybridization instrument
CN108642159A (en) * 2018-06-27 2018-10-12 上海百傲科技股份有限公司 Biological chips detection system
CN108760686A (en) * 2018-08-07 2018-11-06 李浩元 Scattered light urbidmetry detects micro-fluidic chip and the biochemical immunity machine using the chip
CN209537481U (en) * 2018-12-29 2019-10-25 西南民族大学 Membrane DNA chip hybridization instrument

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102134552A (en) * 2010-12-31 2011-07-27 福建泰普生物科学有限公司 Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same
CN104946510A (en) * 2014-03-31 2015-09-30 博奥生物集团有限公司 Nucleic acid amplification/microarray detection integrated microfluidic device
CN103949292A (en) * 2014-04-04 2014-07-30 北京贝泰科技有限公司 Multi-target microarray chip full-automatic hybrid cleaning dyeing system
CN107299054A (en) * 2017-01-12 2017-10-27 武汉菲思特生物科技有限公司 The control system and control method of DNA sequencing device
CN207498376U (en) * 2017-09-26 2018-06-15 南京岚煜生物科技有限公司 The micro-fluidic nucleic acid detection chip of more flux of active control flow path
CN108641902A (en) * 2018-06-08 2018-10-12 四川华汉三创生物科技有限公司 Hybridization instrument and molecular detection system
CN108642142A (en) * 2018-06-08 2018-10-12 四川华汉三创生物科技有限公司 Cartridge component and hybridization instrument
CN108642159A (en) * 2018-06-27 2018-10-12 上海百傲科技股份有限公司 Biological chips detection system
CN108760686A (en) * 2018-08-07 2018-11-06 李浩元 Scattered light urbidmetry detects micro-fluidic chip and the biochemical immunity machine using the chip
CN209537481U (en) * 2018-12-29 2019-10-25 西南民族大学 Membrane DNA chip hybridization instrument

Similar Documents

Publication Publication Date Title
US8431089B2 (en) Process analysis system with sterile sampling of mechanically-sensitive material from a bioreactor
CN106733982B (en) A kind of genetic engineering test tube cleaning device
US4696902A (en) Modular apparatus for cell culture
CN104677675A (en) Sampling device and biological reaction device with sampling device
CN107641596A (en) Multichannel automatic ration sterile sampling device and the bioreactor with the device
CN109283033A (en) A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing
CN209537481U (en) Membrane DNA chip hybridization instrument
CN109444028A (en) A kind of particle analyzer and its liquid channel system, discharging of waste liquid method
CN203965191U (en) Sampler and there is the biological reaction apparatus of this sampler
CN110186737A (en) A kind of liquid-based specimens film-making dyeing all-in-one machine
RU84381U1 (en) DEVICE FOR AUTOMATED ISOLATION OF NUCLEIC ACIDS
CN108753576A (en) A kind of fermentation tank on-line period dilution system and its technique
CN105969651A (en) Microorganism oscillating cleaning and recycling system
CN109576150A (en) Membrane DNA chip hybridization instrument
CN210875403U (en) Full-automatic liquid feeding device
CN209311186U (en) A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing
CN112525611A (en) Sampling device and sampling method for fermentation tank
CN208632519U (en) A kind of fermentor on-line period dilution system
LU100171B1 (en) Device for processing a liquid sample
JP2023549325A (en) Systems and methods for sterile sampling and liquid addition
CN207845644U (en) Multichannel automatic ration sterile sampling device and bioreactor with the device
CN210913874U (en) Inspection waste liquid collecting and processing device for medical inspection
CN219194964U (en) In situ hybridization appearance liquid way
CN205669026U (en) A kind of microorganism concussion clean and reuse system
CN205594014U (en) Full -automatic water quality analyzer's water supply system

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination