CN108760686A - Scattered light urbidmetry detects micro-fluidic chip and the biochemical immunity machine using the chip - Google Patents
Scattered light urbidmetry detects micro-fluidic chip and the biochemical immunity machine using the chip Download PDFInfo
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- CN108760686A CN108760686A CN201810889786.5A CN201810889786A CN108760686A CN 108760686 A CN108760686 A CN 108760686A CN 201810889786 A CN201810889786 A CN 201810889786A CN 108760686 A CN108760686 A CN 108760686A
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- 230000036039 immunity Effects 0.000 title claims abstract description 18
- 238000010790 dilution Methods 0.000 claims abstract description 73
- 239000012895 dilution Substances 0.000 claims abstract description 73
- 238000001514 detection method Methods 0.000 claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000006243 chemical reaction Methods 0.000 claims abstract description 35
- 238000002156 mixing Methods 0.000 claims abstract description 32
- 238000005119 centrifugation Methods 0.000 claims description 27
- 239000012530 fluid Substances 0.000 claims description 22
- 230000005540 biological transmission Effects 0.000 claims description 16
- 238000007493 shaping process Methods 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 9
- 230000007246 mechanism Effects 0.000 claims description 8
- 230000005611 electricity Effects 0.000 claims description 3
- 238000004879 turbidimetry Methods 0.000 abstract description 14
- 238000004737 colorimetric analysis Methods 0.000 abstract description 9
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 238000012360 testing method Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 63
- 238000000034 method Methods 0.000 description 13
- 238000010586 diagram Methods 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- UVXCXZBZPFCAAJ-UHFFFAOYSA-N arc-111 Chemical compound C1=C2OCOC2=CC2=C(N(CCN(C)C)C(=O)C3=C4C=C(C(=C3)OC)OC)C4=CN=C21 UVXCXZBZPFCAAJ-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000013142 basic testing Methods 0.000 description 1
- 238000010256 biochemical assay Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 230000009347 mechanical transmission Effects 0.000 description 1
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- 230000000630 rising effect Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Abstract
The present invention provides scattered light urbidmetry detection micro-fluidic chip and use the biochemical immunity machine of the chip, belong to testing equipment field, including ontology, mixing channel and reaction aperture, ontology is equipped with sample liquid feeding slot, sample quantitative slot and sample overflow launder, sample liquid feeding slot is connected to sample quantitative slot, sample quantitative slot is connected to sample overflow launder, dilution liquid feeding slot is additionally provided on ontology, dilution quantitative slot and dilution overflow launder, dilution liquid feeding slot is connected to dilution quantitative slot, dilution quantitative slot is connected to dilution overflow launder, sample quantitative slot and dilution quantitative slot are connected to mixing channel, mixing channel is combined by runner and reacts aperture connection, it is plane to react aperture close to the side in the ontology center of circle, ontology with react aperture outer ring corresponding position be evagination arc.The present invention effectively controls the diverging of scattered light urbidmetry detection light, detects light intensity sensitivity enhancement, can be used for colorimetric method and turbidimetry is detected.
Description
Technical field
The invention belongs to testing equipment fields, are related to biochemical and immunoassay device more particularly to scattered light urbidmetry detection
Micro-fluidic chip and the biochemical immunity machine for using the chip.
Background technology
Microfluidic chip technology is that accurate manipulation and control nanoliter and picoliters magnitude fluid are (raw in the runner of micro-meter scale
Object sample fluid) new technology, using this technology can sample preparation involved in chemistry and the fields such as biology, reaction,
The basic operation units such as separation, detection and cell culture, sorting, cracking are integrated or are integrated into one piece several square centimeters substantially (very
To smaller) chip on, network is formed by fluid channel, whole system is run through with controllable fluid, to replace conventional chemical or
A kind of technology platform of biology laboratory various functions, the essential characteristic and sharpest edges of Microfluid based Lab on a chip are a variety of lists
First technology flexible combination, scale on whole controllable small platform is integrated.
There are many kinds of immune and biochemistry detection methods, and colorimetric method is the main detection method of biochemistry detection, and detection is former
Reason is a kind of analysis method set up to the selective absorbing of light based on solution, and also known as extinction brightness method, coloring matter is molten
The color of liquid is related with its concentration, using the depth of optics comparison solution color, can measure the concentration of solution.
Turbidimetry is the method that predominantly detects of immune detection, and turbidimetry is divided into that transmittance is turbid and two methods of scattering turbidimetry,
The basic principle of turbidimetry is after antigen-antibody combines, to form immune complex, and compound is polymerize within a certain period of time
Existing turbidity.It when light passes through solution, can be absorbed by immune complex, immune complex amount is more, and the absorbed amount of light exists
Directly proportional to the amount of immune complex in a certain range, the testing principle of colorimetric method and turbidimetry is as shown in Figure 1；Such as Fig. 2
Shown, the basic testing principle of scattered light urbidmetry is to work as the light of certain wavelength along trunnion axis irradiation, makes to encounter antigen by solution
Antibody complex particle, light are reflected by particle granules, are deflected, the wavelength and antigen of the angle and transmitting light of light deflection
Antibody complex granular size and how many closely related.The intensity for scattering light is directly proportional to the content of compound, i.e. determined antigen
More multiple scattering light is also stronger, and the monochromatic source of sense channel and light detection recipient are generally in 5 degree to 90 degree in the method
Angle is arranged.
There are the biochemistry detection chip based on microflow control technique, the chip to utilize microfluidic chip technology currently on the market, it will
The various cuvettes of biochemical reaction are integrated into the reagent reacting hole of circular discs border, may be implemented to utilize colorimetric method and transmission
The biochemistry detecting item and immune detection project of turbidimetry detection, but the chip is when being scattered turbidimetry and being detected,
Since the reagent reaction aperture of chip is cylindric, it is saturating that the inner wall for detecting aperture with the outer wall of chip forms a similar plano-concave
The structure of mirror, the structure can play the role of diverging to the light of scattering, cause detector when detecting scattered light intensity, some
Light intensity can't detect, inadequate for the sensitivity of energy lower scattered light intensity detection, can influence entire detecting system
Performance indicator.
Invention content
The problem to be solved in the present invention is to be to provide scattered light urbidmetry detection micro-fluidic chip and the life using the chip
Change immunization machine, the effective diverging for controlling scattered light urbidmetry detection light detects light intensity sensitivity enhancement, can be used for colorimetric method and
Turbidimetry is detected.
In order to solve the above technical problems, the technical solution adopted by the present invention is：Scattered light urbidmetry detect micro-fluidic chip and
Using the biochemical immunity machine of the chip, including ontology, if mixing channel on the body and multiple reaction apertures, the mixing channel is logical
Cross runner combination with it is described react aperture connection, it is described react aperture close to the ontology center of circle side be plane, described
Body is with the reaction aperture far from the arc that the corresponding position in ontology center of circle side is outwardly convex.
Further, the ontology is equipped with sample liquid feeding slot, sample quantitative slot and sample overflow launder, the sample liquid feeding
Slot is connected to the sample quantitative slot by fluid channel, and the upper end of the sample quantitative slot is connected to the sample overflow launder, institute
State and be additionally provided with dilution liquid feeding slot, dilution quantitative slot and dilution overflow launder on ontology, the dilution liquid feeding slot with it is described
Dilution quantitative slot is connected to by fluid channel, and the upper end of the dilution quantitative slot is connected to the dilution overflow launder, described
Mixing channel is set on the body and with the sample liquid feeding slot in homonymy, and the sample quantitative slot and dilution quantitative slot are logical
Fluid channel is crossed to be connected to the mixing channel.
Further, runner combination includes an annular channel and multiple radial flow paths, the annular channel and institute
It states mixing channel to be connected to by fluid channel, the annular channel is coaxially disposed with the ontology, and each reaction aperture passes through one
A radial flow path is connected to the annular channel.
Further, the radial flow path is arranged perpendicular to the annular channel, and the reaction aperture is far from the ontology
The side of outer ring is circular shape.
Further, the sample overflow launder and dilution overflow launder are set as the same setting circle with the aperture that reacts
On, the quantity of the dilution overflow launder is more than the quantity of the sample overflow launder.
Further, the quantity of the sample overflow launder and dilution overflow launder and the 1/4 of the small hole number of the reaction is accounted for
~1/5, the sample overflow launder and dilution overflow launder are disposed adjacent.
Further, the side of each reaction aperture is equipped with identification code, each sample overflow launder and dilution
The side of hydrorrhea chute is also equipped with identification code.
Further, position corresponding with the reaction aperture is equipped with the shaping area being recessed inwardly on the ontology, described
Shaping area is U-shaped of the opening to the ontology outer ring, and the bottom surface of the shaping area is equipped with the optically focused arc of outwardly convex, the optically focused
The radian of arc is more than the radian of the outer ring circular portion of ontology described in the shaping area.
Detect the biochemical immunity machine of micro-fluidic chip using the turbid method of scattering arm, including left socle, right support, centrifugation motor and
Micro-fluidic chip, the centrifugation motor is located among the left socle and right support and is fixedly connected with the two, described micro-fluidic
Chip level is arranged and is fixedly connected with the force-output shaft of the centrifugation motor, and the upper end at the micro-fluidic chip edge is correspondingly provided with
Transmitted light source module and scattering light source module, the transmitted light source module and scattering light source module shift to install, the transmitted light
The lower end matching of source module is provided with transmission acquisition module, and the working end of the transmission acquisition module is located at the micro-fluidic chip
Lower section, the lower end matching of the scattering light source module is provided with scattering acquisition module, the scattering acquisition module with it is described micro-
The outer ring of fluidic chip is correspondingly arranged.
Further, the transmitted light source module and scattering light source module, which misplace 90 degree, is arranged, the transmitted light source module
It is located on lamp bracket, the lamp bracket is erected between the left socle and right support, and the transmission acquisition module is located at the left branch
It is fixedly connected between frame and right support and with the two, the scattering light source module and scattering acquisition module are each provided at the right support
On.
Further, between the left socle and the right side between be connected with temperature-controlled top plate and temperature control lower plate, the temperature-controlled top plate
Horizontally disposed with temperature control lower plate, the temperature-controlled top plate face is located at the micro-fluidic surface, the temperature-controlled top plate and institute
It states the corresponding part of transmitted light source module and keeps away a hole, temperature-controlled top plate portion corresponding with the scattering light source module equipped with first
It is arranged with second and keeps away a hole, the temperature control lower plate is located at the lower section of the micro-fluidic chip.
Further, the micro-fluidic chip is connected and disconnected from the centrifugation motor by locking device realization, described
Locking device is mechanical grip structure.
Further, the locking device includes locking jaw, actuating arm, drive block, fixed block and drive rod, the fixation
Block is fixedly connected with centrifugation motor, and the relatively described fixed block of the drive block slides up and down connection, the lower end of the drive block and
The drive rod connection, the drive rod drive oscilaltion, the both sides of the drive block upper end symmetrically hinged by cam mechanism
One end of the actuating arm, the other end of the actuating arm are hinged the middle part of the locking jaw, and one end of the locking jaw is hinged
On the fixed block, the other end is extended to one end far from the fixed block, and the symmetrically arranged locking jaw is at opening
The upward tubaeform setting of mouth.
Further, the cam mechanism includes plate cam and lifting motor, and the lifting motor solid lock is on the left side
On holder, the lifting motor drives the plate cam to rotate by gear structure, and the plate cam is vertically arranged, described
The lower end of drive rod is equipped with roller, and the roller contacts setting with the outer ring of the plate cam.
Further, the micro-fluidic chip is located at on export frame, and the both sides into export frame are equipped with rack, with institute
That states rack engagement setting has driving gear, and the driving gear is driven by disengaging motor to be rotated, and the disengaging motor solid lock exists
On the right support.
Further, the lower end into export frame is equipped with guide groove, and the centrifugation motor is by motor cabinet solid lock in institute
State between left socle and right support, the upper end of the motor cabinet is equipped with guide post, the guide post be located in the guide groove and
The two is equipped with.
Compared with prior art, the invention has the advantages and positive effects that：1, the outer ring of ontology of the present invention is circle,
It is plane to react aperture close to the side of ontology outer ring, this plane forms planoconvex spotlight with the round of ontology outer ring, is conducive to more
More light polymerizations are received by recipient, promote the sensitivity of detection；2, sample overflow launder and dilution overflow launder are disposed adjacent,
The convenient flooded conditions in same position observation sample and dilution react aperture concentrated setting, convenient during detection
Centralized detecting searches without walking, promotes the convenience of operation；It 3,, also can be to each anti-after ontology rotation after setting code
It answers aperture to be identified and distinguish, while being also convenient for distinguishing reaction aperture, sample overflow launder and dilution overflow launder, after differentiation,
It avoids confusion, is detected for reaction aperture, the scattered light intensity of liquid works as sample when practical application after judgement mixing
This overflow launder and dilution overflow launder could be mixed after having the part of overflow, ensure sample quantitative slot and dilution quantitative slot
Interior liquid is filled with state；5, micro-fluidic chip is arranged on centrifugation motor, realizes centrifuging, is quantitative, being dilute for sample-adding liquid
It releases and mixes, while transmitted light source module and scattering light source module work are set simultaneously in the edge of micro-fluidic chip, and
Setting transmission acquisition module and scattering acquisition module, can scatter and transmit the transmission for being carried out at the same time detection and carrying out detection data
Analysis, function carries out integrally disposed, a tractor serves several purposes, resource-effective, saves the use cost of user, in combination with micro-fluidic chip one
It is secondary to detect a sample individual event/Multiple biomarkers, individual event/multiple immune indexes, also can one-time detection go out the multinomial life of a sample
Change index+multiple immune indexes, detection efficiency greatly promotes；6, temperature-controlled top plate and temperature control lower plate are set, it can be to micro-fluidic chip
Temperature controlled, many liquid that are loaded have temperature certain requirement, therefore need during detection to ensure temperature；
7, micro-fluidic chip is realized with centrifugation motor by locking device and detaches and lock automatically, improves the effect placed in detection process
Rate, while micro-fluidic chip is located at into can pass in and out on export frame and with into export frame, is facilitated sample-adding, is improved the facility of operation
Property.
Description of the drawings
The attached drawing for constituting the part of the present invention is used to provide further understanding of the present invention, schematic reality of the invention
Example and its explanation are applied for explaining the present invention, is not constituted improper limitations of the present invention.In the accompanying drawings：
Fig. 1 is colorimetric method and the detection principle diagram of turbidimetry；
Fig. 2 is the basic detection principle diagram of scattered light urbidmetry；
Fig. 3 is the planar structure of scattered light urbidmetry detection micro-fluidic chip of the present invention and the biochemical immunity machine using the chip
Figure；
Fig. 4 is scattered light urbidmetry detection micro-fluidic chip of the present invention and the biochemical immunity machine embodiment A using the chip
Partial structural diagram；
Fig. 5 is the scatter diagram of light during Fig. 3 of the present invention actually detected；
Fig. 6 is scattered light urbidmetry detection micro-fluidic chip of the present invention and the biochemical immunity machine embodiment B using the chip
Partial structural diagram；
Fig. 7 is the scatter diagram of light during Fig. 5 of the present invention actually detected；
Fig. 8 is structural schematic diagram of the present invention using the biochemical immunity machine of the turbid method detection micro-fluidic chip of scattering arm；
Fig. 9 be the present invention using the biochemical immunity machine of scattering arm turbid method detection micro-fluidic chip without right support, into export
The structural schematic diagram of frame and transmitted light source module；
Figure 10 is the structural schematic diagram of present invention centrifugation motor and motor cabinet cooperation；
Figure 11 is the principle mechanism figure of locking device of the present invention；
Figure 12 is that the light of present invention scattering detection moves towards schematic diagram；
Figure 13 is that the light of present invention transmission detection moves towards schematic diagram；
Figure 14 is the schematic diagram that micro-fluidic chip of the present invention is carried out at the same time scattering and transmission.
Reference numeral：
1- ontologies；The shaping areas 11-；111- optically focused arcs；2- sample liquid feeding slots；21- sample quantitative slots；22- sample overflow launders；
3- dilution liquid feeding slots；31- dilution quantitative slots；32- dilution overflow launders；4- mixing channels；5- reacts aperture；51- identification codes；
52- annular channels；53- radial flow paths；6- receivers；81- left socles；82- right supports；83- micro-fluidic chips；84- centrifugation electricity
Machine；841- motor cabinets；842- guide posts；85- transmitted light source modules；851- lamp brackets；852- transmits acquisition module；86- scatters light
Source module；861- scatters acquisition module；87- temperature-controlled top plates；871- first keeps away a hole；872- second keeps away a hole；Under 87 '-temperature controls
Plate；88- is into export frame；881- racks；882- drives gear；883- passes in and out motor；9- locking devices；91- fixed blocks；92- drives
Motion block；93- actuating arms；94- locking jaws；95- drive rods；96- rollers；97- plate cams；98- lifting motors.
Specific implementation mode
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
In the description of the present invention, it is to be understood that, term "center", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, with specific azimuth configuration and operation, therefore should not be understood as pair
The limitation of the present invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can
To express or implicitly include one or more this feature.In the description of the present invention, unless otherwise indicated, " multiple "
It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
Can also be electrical connection to be mechanical connection；It can be directly connected, can also indirectly connected through an intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
Specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings.
As shown in figure 3, the present invention, which is scattered light urbidmetry, detects micro-fluidic chip and the biochemical immunity machine using the chip, packet
It includes ontology 1, mixing channel 4 and reaction aperture 5, ontology 1 and is equipped with sample liquid feeding slot 2, sample quantitative slot 21 and sample overflow launder 22,
Sample liquid feeding slot 2 is connected to sample quantitative slot 21 by fluid channel, and the upper end of sample quantitative slot 21 is connected to sample overflow launder 22,
Ensure that sample flows between sample liquid feeding slot 2, sample quantitative slot 21 and sample overflow launder 22 successively, is additionally provided on ontology 1 dilute
Liquid liquid feeding slot 3, dilution quantitative slot 31 and dilution overflow launder 32 are released, dilution liquid feeding slot 3 passes through with dilution quantitative slot 31
Fluid channel is connected to, and the upper end of dilution quantitative slot 31 is connected to dilution overflow launder 32, ensures that dilution adds in dilution successively
Flowed between liquid bath 3, dilution quantitative slot 31 and dilution overflow launder 32, mixing channel 4 be located on ontology 1 and with sample liquid feeding slot
2 are connected to by fluid channel with mixing channel 4 in homonymy, sample quantitative slot 21 and dilution quantitative slot 31, and mixing channel 4 passes through runner
Combination is connected to aperture 5 is reacted, and the quantity of reaction aperture 5 is outer rings that are multiple and being each provided at mixing channel 4, after the rotation of ontology 1,
Under the action of inertia, the liquid in mixing channel 4 is reacted by runner combined flow channel in aperture 5, and reaction aperture 5 is close to ontology 1
The side in the center of circle be plane, ontology 1 and react corresponding position of the aperture 5 far from 1 reason side of ontology for outside figure arc,
This plane forms planoconvex spotlight with the round of 1 outer ring of ontology, is conducive to more light polymerizations and is received by receiver 6, promotes inspection
The sensitivity of survey；It is highly preferred that ontology 1 is circle.
Preferably, runner combination includes an annular channel 52 and multiple radial flow paths 53, annular channel 52 and mixing channel 4
It is connected to by fluid channel, annular channel 52 is coaxially disposed with ontology 1, and each aperture 5 that reacts passes through a radial flow path 53 and ring
Shape runner 52 is connected to, and facilitates the connection of multiple reaction apertures 5 and mixing channel 4, this structure can be symmetrical arranged, easy to process, beautiful；
It is highly preferred that radial flow path 53 is arranged perpendicular to annular channel 52, just the liquid in mixing channel 4 is more rapidly for 1 rear swivel of ontology
Enter in reaction aperture 5, reaction side of the aperture 5 far from 1 outer ring of ontology is circular shape, easy to process, and is helped
In promotion scattering strength.
Preferably, sample overflow launder 22 and dilution overflow launder 32 are set as with aperture 5 is reacted on the same setting circle, are added
Work is convenient, good appearance, and the quantity of dilution overflow launder 32 is more than the quantity of sample overflow launder 22, and the spillway discharge of sample is less than dilute
Release the spillway discharge of liquid, specific aim setting, the utilization rate of lifting means.
Preferably, the quantity of sample overflow launder 22 and dilution overflow launder 32 and account for reaction 5 quantity of aperture 1/4~1/5,
Sample overflow launder 22 and dilution overflow launder 32 are disposed adjacent, the convenient overflow feelings in same position observation sample and dilution
Condition, reacts 5 concentrated setting of aperture, and convenient centralized detecting during detection searches without walking, promotes the facility of operation
Property.
Preferably, each the side of reaction aperture 5 is equipped with identification code 51, each sample overflow launder 22 and dilution overflow
The side of slot 32 is also equipped with identification code 51, after setting code, after ontology 1 rotates, can also know to each reaction aperture 5
It not and distinguishes, while being also convenient for distinguishing reaction aperture 5, sample overflow launder 22 and dilution overflow launder 32, after differentiation, avoid mixing
Confuse, is detected for reaction aperture 5, the scattered light intensity of liquid after judgement mixing, when practical application, when sample overflow
Slot 22 and dilution overflow launder 32 could be mixed after having the part of overflow, ensure sample quantitative slot 21 and dilution quantitative slot
Liquid in 31 is filled with state.
Embodiment A：As shown in Figure 4 and Figure 5, the inner wall of reaction aperture 5 is held round small for the side close from 1 center of circle of ontology
Planar structure is made in the radian in hole, the side far from the center of circle, the cambered structure that the outer ring edge of ontology 1 is held round, anti-in this way
It answers and forms a kind of structure of planoconvex spotlight at side of the aperture 5 far from circle centre position and 1 outmost turns of ontology, this structure not only can be with
For the Reagent Project that colorimetric method and turbidimetry are detected, and it can avoid the divergence problem of scattering light.
Embodiment B：As shown in Figure 6 and Figure 7, the inner wall of reaction aperture 5 is that the side close from the center of circle is held round aperture
Planar structure is made in radian, the side far from the center of circle, on ontology 1 position corresponding with reaction aperture 5 be equipped be recessed inwardly at
Type area 11, shaping area 11 are U-shaped of the opening to 1 outer ring of ontology, and the bottom surface of shaping area 11 is equipped with the optically focused arc 111 of outwardly convex,
The radian of optically focused arc 111 is more than the radian of the outer ring circular portion of 11 ontology 1 of shaping area, can enhance the aggregation journey to scattering light
Degree, the Reagent Project that this structure can be not only used for colorimetric method and turbidimetry is detected, and can realize scattering light
Aggtegation, so that detecting element is received more scattering lights in smaller space, improve the sensitivity of detecting system.
The biochemical immunity machine of micro-fluidic chip is detected using the turbid method of scattering arm, as shown in Fig. 8, Fig. 9 and 10, the present invention is base
In micro-fluidic biochemistry and immune integrated detection machine, including left socle 81, right support 82, centrifugation motor 84 and micro-fluidic chip
83, centrifugation motor 84 is located among left socle 81 and right support 82 and is fixedly connected with the two, and micro-fluidic chip 83 is horizontally disposed
And be fixedly connected with the force-output shaft of centrifugation motor 84, the upper end at 83 edge of micro-fluidic chip is correspondingly provided with 85 He of transmitted light source module
Light source module 86 is scattered, transmitted light source module 85 and scattering light source module 86 shift to install, the lower end of transmitted light source module 85
With transmission acquisition module is provided with, the working end of transmission acquisition module 852 is located at the lower section of micro-fluidic chip 83, scatters light source die
The lower end matching of block 86 is provided with scattering acquisition module, and the outer ring of scattering acquisition module and micro-fluidic chip 83 is correspondingly arranged.
Preferably, transmitted light source module 85 and scattering light source module 86, which misplace 90 degree, is arranged, and transmitted light source module 85 is located at
On lamp bracket 851, lamp bracket 851 is erected between left socle 81 and right support 82, and transmission acquisition module 852 is located at left socle 81 and the right side
It is fixedly connected between holder 82 and with the two, scattering light source module 86 and scattering acquisition module 861 are each provided on right support 82, wrong
Position is arranged at 90 degree, and structure is easy layout, compacter.
Preferably, between left socle 81 and the right side between be connected with temperature-controlled top plate 87 and temperature control lower plate 87 ', 87 He of temperature-controlled top plate
Temperature control lower plate 87 ' is horizontally disposed, and 87 face of temperature-controlled top plate is located at micro-fluidic surface, temperature-controlled top plate 87 and transmitted light source mould
A hole 871 is kept away in 85 corresponding part of block equipped with first, and the part corresponding with scattering light source module 86 of temperature-controlled top plate 87 is equipped with second
A hole 872 is kept away, temperature control lower plate 87 ' is located at the lower section of micro-fluidic chip 83, and temperature-controlled top plate 87 and temperature control lower plate 87 ' is arranged, can be right
The temperature of micro-fluidic chip 83 is controlled, and many liquid that are loaded have temperature certain requirement, therefore during detection
It needs to ensure temperature, it is highly preferred that left socle 81 is equipped with thermal switch, for incuding the temperature of micro-fluidic chip 83.
Preferably, micro-fluidic chip 83 is connected and disconnected from centrifugation motor 84 by the realization of locking device 9, locking device 9
For mechanical grip structure, pneumatic type can be used in mechanical grip structure, identical as the pneumatic driving dynamic formula clamping jaw principle on lathe.
Preferably, as shown in figure 11, locking device 9 includes locking jaw 94, actuating arm 93, drive block 92,91 and of fixed block
Drive rod 95, fixed block 91 are fixedly connected with centrifugation motor 84, and drive block 92 is relatively fixed block 91 and slides up and down connection, drive block
92 lower end is connect with drive rod 95, and drive rod 95 drives oscilaltion, the both sides of 92 upper end of drive block symmetrical by cam mechanism
One end of hinged actuating arm 93, the other end of actuating arm 93 are hinged the middle part of locking jaw 94, and one end of locking jaw 94 is hinged on admittedly
Determine on block 91, the other end is extended to one end far from fixed block 91, symmetrically arranged locking jaw 94 at opening upwards loudspeaker
Shape is arranged, and during drive block 92 moves up and down, actuating arm 93 is driven to move up and down, and then realize the opening of locking jaw 94
And closure, locking jaw 94 are arranged in the interstitial hole of micro-fluidic chip 83, under 94 open configuration of locking jaw, with micro-fluidic chip 83
It fits closely, realizes the rotation together with centrifugation motor 84, complete centrifugation, quantitative, dilution, mixing action, needed after the completion of detection
When clearing up or replace sample-adding product, drive block 92 declines, and actuating arm 93 drives locking jaw 94 to retract, micro-fluidic chip
83 are detached from centrifugation motor 84, facilitate and take.
Preferably, cam mechanism includes plate cam 97 and lifting motor 98,98 solid lock of lifting motor on left socle 81,
Lifting motor 98 drives plate cam 97 to rotate by gear structure, and plate cam 97 is vertically arranged, and the lower end of drive rod 95 is set
There are roller 96, roller 96 to contact setting with the outer ring of plate cam 97, plate cam 97 is realized under the drive of lifting motor 98
Rotation, and then 96 oscilaltion of roller coordinated with it is driven, the oscilaltion of drive rod 95 is completed, it is this using cam mechanism
Mechanical transmission, stability is high, and failure rate is low.
Preferably, micro-fluidic chip 83 is located at on export frame 88, rack 881 is equipped with into the both sides of export frame 88, with tooth
The engagement setting of item 881 has driving gear 882, and driving gear 882 is driven by disengaging motor 883 to be rotated, and 883 solid lock of motor is passed in and out
On right support 82, disengaging motor 883 rotates, and driving gear 882 is engaged with rack 881, realizes the movement into export frame 88,
Facilitate micro-fluidic chip 83 take and sample-adding action；It is highly preferred that being equipped with guide groove, centrifugation electricity into the lower end of export frame 88
For machine 84 by 841 solid lock of motor cabinet between left socle 81 and right support 82, the upper end of motor cabinet 841 is equipped with guide post 842, leads
It is located in guide groove to column 842 and the two is equipped with, after guide post 842 and guide groove are set, ensure that and moved into export frame 88
Dynamic precision and stability realizes the stability of the operating of micro-fluidic chip 83.
In actual application process, as shown in Figure 12, Figure 13 and Figure 14, after starting device, start disengaging motor first
883, it will remove, then sample be increased to micro-fluidic chip 83 in micro-fluidic chip 83 to, then into export into export frame 88
Frame 88 is moved to the inside of equipment so that micro-fluidic chip 83 reaches the specified position of equipment, and lifting motor 98 starts, and tablet is convex
Wheel 97 realizes the rising of drive rod 95 by roller 96, and locking jaw 94 is opened to be integrated with the tensioning of micro-fluidic chip 83, micro-fluidic
Chip 83 follow centrifugation motor 84 rotate together, complete sample-adding liquid centrifuge, quantify, diluting, mixing and heating act, then
Equipment starts exposure tests, and scattering turbidimetry detection intersects progress, transmitted light source module 85 and scattering with transmission colorimetric, than turbid detection
Light source module 86 works, and transmits acquisition module 852 and scatters after light data are collected by the work of acquisition module 861 simultaneously inspection
Measured data by plug-in calculates output pattern detection as a result, the plug-in of this detection is known module in the market, can
Directly purchase uses, and total is carried out at the same time scattering and transmission detection, and function carries out integrally disposed, a tractor serves several purposes, resource section
About, the use cost of user is saved, goes out a sample individual event/Multiple biomarkers, list in combination with 83 one-time detection of micro-fluidic chip
Item/multiple immune indexes, also can one-time detection go out a sample Multiple biomarkers+multiple immune indexes, detection efficiency carries significantly
It rises
Micro-fluidic chip is loaded operate during, operation according to the following steps is completed, 1, in sample pipetting volume slot
Enough samples to be checked are added, enough dilutions are included in dilution loading slot；2, sample application slot passes through fluid channel and sample
This quantitative slot 21 is connected to, and dilution loading slot is connected to by fluid channel with dilution quantitative slot 31, and centrifugation motor 83, which starts, drives this
Body 1 rotates, and sample to be checked enters sample quantitative slot 21 and quantifies, and excessive sample enters in sample overflow launder 22, and dilution enters
Dilution quantitative slot 31 is quantitative, and excessive dilution enters in dilution overflow launder 32；3, sample quantitative slot 21 passes through fluid channel
Be connected to mixing channel 4, dilution quantitative slot 31 is connected to by fluid channel with mixing channel 4, the sample and dilution quantitatively completed into
Enter and mixed according to quantitative ratio in mixing channel 4, the setting of ratio can be conveyed by setting the fluid channel diameter of sample with
The ratio for conveying the fluid channel diameter of dilution is completed；4, reagent reaction aperture 5 is radial by an annular channel 52 and one group
Runner 53 is connected to mixing channel 4, and diluted sample to be tested enters reagent reaction aperture 5 and reacted by a certain percentage；5, light
After being received by receiver 6, the transmission of aperture 5 and scattered light intensity are reacted by detection reagent, detect predetermined substance in sample to be tested
Concentration.When being detected using scattered light urbidmetry, the sensitivity and detection of the detecting system that the power of scattering light directly affects
The range of linearity, this structure is mainly for disperse function of the convex lens structures to scattered light intensity for reacting the formation of aperture 5 so that one
The problem of scattered light intensity divided cannot be detected by the detector, it is proposed that reagent reacts the planoconvex spotlight structure of aperture 5, convex lens
Structure can realize the aggregation of scattering light, and detecting element can be made to detect more light intensity in smaller detection interval,
The Reagent Project that the structure can be not only used for colorimetric method and turbidimetry is detected, and can effectively avoid scattering
The divergence problem of light significantly improves the sensitivity that detecting element receives scattered light intensity.
One embodiment of the present invention has been described in detail above, but the content be only the present invention preferable implementation
Example should not be construed as limiting the practical range of the present invention.It is all according to all the changes and improvements made by the present patent application range
Deng should all still fall within the scope of the patent of the present invention.
Claims (10)
1. scattered light urbidmetry detects micro-fluidic chip, it is characterised in that：Including ontology, if mixing channel on the body and multiple anti-
Aperture, the mixing channel is answered to combine by runner and be connected to the aperture that reacts, the reaction aperture is close to the ontology center of circle
Side be plane, the ontology is outwardly convex far from the corresponding position in ontology center of circle side with the reaction aperture
Arc.
2. scattered light urbidmetry according to claim 1 detects micro-fluidic chip, it is characterised in that：The ontology is equipped with sample
This liquid feeding slot, sample quantitative slot and sample overflow launder, the sample liquid feeding slot are connected to the sample quantitative slot by fluid channel,
The upper end of the sample quantitative slot is connected to the sample overflow launder, and dilution liquid feeding slot, dilution are additionally provided on the ontology
Quantitative slot and dilution overflow launder, the dilution liquid feeding slot is connected to the dilution quantitative slot by fluid channel, described dilute
The upper end for releasing liquid quantitative slot is connected to the dilution overflow launder, and the mixing channel sets on the body and adds with the sample
Liquid bath is connected to by fluid channel with the mixing channel in homonymy, the sample quantitative slot and dilution quantitative slot.
3. scattered light urbidmetry according to claim 2 detects micro-fluidic chip, it is characterised in that：The sample overflow launder and
Dilution overflow launder is set as with the aperture that reacts on the same setting circle, and the quantity of the dilution overflow launder is more than described
The quantity of the quantity of sample overflow launder, the sample overflow launder and dilution overflow launder and account for the 1/4 of the small hole number of the reaction
~1/5, the sample overflow launder and dilution overflow launder are disposed adjacent；
The side of each reaction aperture is equipped with identification code, the side of each the sample overflow launder and dilution overflow launder
Also it is equipped with identification code.
4. scattered light urbidmetry according to claim 1 detects micro-fluidic chip, it is characterised in that：On the ontology with it is described
The corresponding position of reaction aperture is equipped with the shaping area being recessed inwardly, and the shaping area is U-shaped of the opening to the ontology outer ring, institute
The bottom surface for stating shaping area is equipped with the optically focused arc of outwardly convex, and the radian of the optically focused arc is more than the outer of ontology described in the shaping area
Enclose the radian of circular portion.
5. scattered light urbidmetry according to claim 1 detects micro-fluidic chip, it is characterised in that：The runner combines
One annular channel and multiple radial flow paths, the annular channel are connected to the mixing channel by fluid channel, the annular flow
Road is coaxially disposed with the ontology, and each reaction aperture is connected to by a radial flow path with the annular channel,
The radial flow path is arranged perpendicular to the annular channel, and the reaction side of the aperture far from the ontology outer ring is arc-shaped
Shape.
6. using the biochemical immunity machine of scattered light urbidmetry detection micro-fluidic chip described in claim 1, it is characterised in that：Including
Left socle, right support, centrifugation motor and micro-fluidic chip, the centrifugation motor be located at the left socle and right support centre and with
The two is fixedly connected, and the micro-fluidic chip is horizontally disposed and is fixedly connected with the force-output shaft of the centrifugation motor, the miniflow
The upper end of control chip edge is correspondingly provided with transmitted light source module and scattering light source module, the transmitted light source module and scattering light source
Module shifts to install, and the lower end matching of the transmitted light source module is provided with transmission acquisition module, the transmission acquisition module
Working end is located at the lower section of the micro-fluidic chip, and the lower end matching of the scattering light source module is provided with scattering acquisition module,
The outer ring of the scattering acquisition module and the micro-fluidic chip is correspondingly arranged.
7. the biochemical immunity machine according to claim 6 for detecting micro-fluidic chip using scattered light urbidmetry, it is characterised in that：
Temperature-controlled top plate and temperature control lower plate are connected between the left socle and the right side, the temperature-controlled top plate and the equal level of temperature control lower plate are set
It sets, the temperature-controlled top plate face is located at the micro-fluidic surface, and the temperature-controlled top plate is corresponding with the transmitted light source module
Part keep away a hole equipped with first, a hole is kept away in temperature-controlled top plate part corresponding with the scattering light source module equipped with second,
The temperature control lower plate is located at the lower section of the micro-fluidic chip.
8. the biochemical immunity machine according to claim 6 for detecting micro-fluidic chip using scattered light urbidmetry, it is characterised in that：
The micro-fluidic chip is connected and disconnected from the centrifugation motor by locking device realization, and the locking device includes locking
Pawl, actuating arm, drive block, fixed block and drive rod, the fixed block are fixedly connected with centrifugation motor, and the drive block is with respect to institute
It states fixed block and slides up and down connection, the lower end of the drive block is connect with the drive rod, and the drive rod is driven by cam mechanism
Dynamic oscilaltion, the both sides of the drive block upper end are symmetrically hinged one end of the actuating arm, the other end hinge of the actuating arm
The middle part of the locking jaw is connect, one end of the locking jaw is hinged on the fixed block, and the other end is to far from the fixed block
One end be extended, the symmetrically arranged locking jaw at opening upwards tubaeform setting.
9. the biochemical immunity machine according to claim 8 for detecting micro-fluidic chip using scattered light urbidmetry, it is characterised in that：
The cam mechanism includes plate cam and lifting motor, and the lifting motor solid lock is on the left socle, the lifting electricity
Machine drives the plate cam to rotate by gear structure, and the plate cam is vertically arranged, and the lower end of the drive rod is equipped with
Roller, the roller contact setting with the outer ring of the plate cam.
10. the biochemical immunity machine according to claim 6 for being detected micro-fluidic chip using scattered light urbidmetry, feature are existed
In：The micro-fluidic chip is located at on export frame, and the both sides into export frame are equipped with rack, and setting is engaged with the rack
Have a driving gear, the driving gear is driven by disengaging motor to be rotated, and the disengaging motor solid lock is on the right support, institute
State into the lower end of export frame and be equipped with guide groove, the centrifugation motor by motor cabinet solid lock the left socle and right support it
Between, the upper end of the motor cabinet is equipped with guide post, and the guide post is located in the guide groove and the two is equipped with.
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| CN201810889786.5A CN108760686A (en) | 2018-08-07 | 2018-08-07 | Scattered light urbidmetry detects micro-fluidic chip and the biochemical immunity machine using the chip |
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