CN208366832U - Complex water body multi-parameter based on microflow control technique examines equipment fastly - Google Patents
Complex water body multi-parameter based on microflow control technique examines equipment fastly Download PDFInfo
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- CN208366832U CN208366832U CN201820700728.9U CN201820700728U CN208366832U CN 208366832 U CN208366832 U CN 208366832U CN 201820700728 U CN201820700728 U CN 201820700728U CN 208366832 U CN208366832 U CN 208366832U
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Abstract
The utility model relates to microfluidic art, discloses a kind of Complex water body multi-parameter based on microflow control technique and examine equipment fastly.It includes chip and detection device that the Complex water body multi-parameter examines equipment fastly；Wherein, the chip includes the reaction tank for example reaction；The detection device is used to detect the signal of the sample in the reaction tank.The Complex water body multi-parameter of the utility model examines that equipment detection speed is fast, and flux is high fastly, has good processing capacity for complex sample.
Description
Technical field
The utility model relates to microfluidic arts, and in particular to a kind of Complex water body based on microflow control technique is joined more
The fast inspection equipment of number.
Background technique
While world economy and industrial expansion are that the mankind bring huge convenient, but also pollutant is emitted on quantity
It is continuously increased in type, environmental issue is on the rise.Greatly developing all kinds of high-precision analysis instruments and detection technique
Meanwhile the analytical technology of small portable, simple and fast also becomes a big research hotspot, it is numerous because operating to supplement large-scale instrument
It is trivial, maintenance workload is larger, requires laboratory environment high, to be difficult to use in on-site test etc. deficiency.
Micro-total analysis system is also known as chip lab, be intended to by laboratory functional one be integrated on a chip
Kind analytical technology, brilliant prospect is shown in terms of the miniaturization of analytical technology, portability, automation, is become in recent years
One big research hotspot.
Micro-total analysis system still cannot actually realize the function of chip lab at present also in developing stage, thus
When involving the need for carrying out the analysis experiment of pre-treatment, pretreatment part experiment is usually first manually completed, enters back into micro- complete point
Analysis system is analyzed, and micro-total analysis system automation, rapid, portability development are limited.
Utility model content
It is asked the purpose of this utility model is to overcome of the existing technology for the high-throughput quickly detection of complex sample
Topic provides a kind of Complex water body multi-parameter based on microflow control technique and examines equipment fastly, which examines equipment inspection fastly
Degree of testing the speed is fast, and flux is high, has good processing capacity for complex sample.
To achieve the goals above, on the one hand the utility model provides a kind of Complex water body based on microflow control technique and joins more
The fast inspection equipment of number, it includes chip and detection device which examines equipment fastly；Wherein, the chip is rotary core
Piece or straight-line chip, the chip include the reaction tank for example reaction；The detection device is described anti-for detecting
The signal of the sample of Ying Chizhong.
Preferably, the label for determining detection signal, the label and the reaction tank pair are provided on the chip
It should be arranged.
Preferably, the multiple reaction tank of the rotary chip is arranged in using the center of the chip as the circle in the center of circle
Zhou Shang.
Preferably, the chip includes substrate and cover plate, and the substrate is fixedly connected with the cover plate, alternatively, the base
Piece and the cover plate can be mutually shifted, to change the connected relation in the chip between reaction tank and/or runner.
Preferably, it further includes sample introduction component that the Complex water body multi-parameter examines equipment fastly, and the sample introduction component is used for sample
Product sample introduction is into the chip.
It is highly preferred that the sample introduction component is manual injector；
It is highly preferred that the sample introduction component includes pretreatment portion, the pretreatment portion is for pre-processing sample.
Preferably, the detection device includes more than one detection part.
It is highly preferred that the detection part be chromaticity sensing section, absorbance detection component, fluorescence signal detection part,
One of Raman signal detection component and infrared spectroscopy detection part are a variety of.
Preferably, it further includes that the driving part of chip rotation, signal is driven to adopt that the Complex water body multi-parameter examines equipment fastly
Collect one of component, data processor, data output component and data transfer component or a variety of.
Through the above technical solutions, the Complex water body multi-parameter of the utility model examine equipment fastly can be according to the needs of detection
Appropriate chip selected to use, detection part, sampling device etc. have the advantages that high-throughput, detection is fast, accuracy is high, for
Various samples are attained by good detection effect, and pretreatment appropriate, reaction process can also be set as needed, simpler
Just detection is quickly completed.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of rotary chip of the utility model；
Fig. 2 is a kind of structural schematic diagram of slidingtype chip of the utility model；
Fig. 3 is a kind of structural schematic diagram of manual injector of the utility model；
Fig. 4 is the structural schematic diagram of the manual injector with pretreatment component of the utility model；
Fig. 5 is the structural schematic diagram of the detection device of the utility model.
Description of symbols
1, manual injector 2, chip
11, control unit 12, sample cell 13, out sample portion
14, pretreatment portion
21, runner 22, reaction tank 23, injection port
31, light source 32, branch optical fiber 33, spectroscopy equipment
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
On the one hand the utility model provides a kind of Complex water body multi-parameter based on microflow control technique and examines equipment fastly, the complexity
It includes chip and detection device that water body multi-parameter examines equipment fastly；Wherein, the chip be rotary chip or straight-line chip,
The chip includes the reaction tank for example reaction；The detection device is used to detect the letter of the sample in the reaction tank
Number.
In the present invention, the Complex water body multi-parameter examines equipment by using microflow control technique fastly, can pass through
Multiple reaction tanks are set on chip, so as to be completed in a short time the synchronous detection of a large amount of samples, and can be passed through
Structure designs in chip, completes the operation such as sample pretreatment, reaction, and detection process Highgrade integration is easy to operate, and precision is high.
According to the utility model, there is no particular limitation for the structure of the chip, can be rotary chip or in line
Formula chip can be the various well known shapes such as circle, rectangle.
As the rotary chip, refer to can on chip a little where chip rotation in surface.From
From the point of view of rotation, the rotary chip is preferably circular or regular polygon, more preferably round.It is described rotary
When chip is round, preferably rotated around its center of circle.
A preferred embodiment according to the present utility model, as shown in Figure 1, the multiple reaction tank is arranged with institute
The center for stating chip is on the circumference in the center of circle.Center of the i.e. the multiple reaction tank apart from the chip is equidistant.
Another preferred embodiment according to the present utility model, the reaction tank are divided into multiple groups, and every group of reaction tank
With multiple reaction tanks, every group of multiple reaction tanks are separately positioned on using the center of the chip as in one group of concentric circles in the center of circle
On one circumference.That is center of every group of the multiple reaction tanks apart from the chip is equidistant.
There is no particular limitation for the rotating manner of the rotary chip, it is preferable that the rotary chip can pass through
It drives either manually or by the driving part in detection device and is rotated in detection device, preferably around the rotary chip
Center rotation.From detection device is simplified, cost is reduced, convenient for from the point of view of portable operation, the rotary chip is excellent
Gating can be manually rotated.And from detection efficiency is improved, it is realized in the short time from the point of view of detecting on a large scale, the rotary core
Piece preferably passes through the driving rotation of the driving part in detection device.
As the straight-line chip, refers to the chip that the reaction tank on chip is arranged in rows, is typically designed to rectangle,
And with reaction tank more than a row.The reaction tank of straight-line chip, generally can be by making straight-line core due to marshalling
Piece is mobile relative to the detection device and is detected, such as the pull straight-line chip in detection device.
In the present invention, the reaction tank on the chip can be 2 or more, preferably 5 or more, more preferably
10-50.Can also include runner on the chip for the ease of reaction, the runner for sample differential responses pond it
Between flow.The set-up mode of the runner needs that chip detection demand is cooperated to carry out, such as needs to carry out multistep in the chips anti-
At once, it can be set to include successively reaction tank-runner-reaction tank-runner-reaction tank structure, thus in multiple reaction tanks
It is middle to complete different reactions.In order to control the flowing of sample, various promotion mixed structures, micro- can also be set in the chips
Valve etc., whether for controlling the mixing and flowing of sample.
A preferred embodiment according to the present utility model is provided on the chip for determining detection signal
Label, the label are correspondingly arranged with the reaction tank.
A preferred embodiment according to the present utility model, as shown in Figure 1, the chip has multiple reaction tanks, it is more
A reaction tank is arranged in using the center of the chip to be correspondingly arranged on more than one circumference in the center of circle with the reaction tank
There is the label for determining detection signal.In the use process of chip, by rotary chip, so that the reaction tank position in chip
In the corresponding inspection positions of detection device, while it can determine corresponding detection part.Preferably, distinguish with each reaction tank
It is correspondingly arranged on the label for determining detection signal, thus a chip can be completed in a short time a variety of different detections and refer to
Target detection.And detection process is easy to operate, and accuracy is high, and can be suitable for various detection methods.
In the present invention, the label can be the label with specific color or shape, such as can pass through
The modes such as printing or stickup are formed on the chip, the groove with specific shape, the notch etc. being also possible on chip.
There is no particular limitation for the shape of the label, achievees the purpose that identify and distinguish between, such as can be triangle, pros
Shape, regular pentagon, star, naturally it is also possible to for number, letter or various irregular shapes.Can only it be arranged on chip
1 above-mentioned label, the label is for determining that the detection signal of chip (can be same detection signal, be also possible to multiple reactions
The detection ordering of unlike signal in pond), thus when carrying out different tests with different chips convenient for determining detection mode；In chip
On can also correspond to one group of even corresponding detection signal of each reaction tank setting, to complete unlike signal in a chip
Detection.
In the present invention, if the set-up mode of the multiple reaction tank make chip rotate after, differential responses pond
The detection zone of detection device can be located at.Justified by the way that the multiple reaction tank is arranged in the center of the chip
On more than one circumference of the heart, so as to complete the detection of multiple reaction tanks on chip with single detection part, reaches and criticize
Measure the purpose of detection.
According to the utility model, the chip includes substrate and cover plate, and the substrate is fixedly connected (also referred to as with the cover plate
For " fixed chip "), alternatively, the substrate and the cover plate can be mutually shifted, to change reaction tank in the chip
And/or the connected relation (also referred to as " slidingtype chip ") between runner.In general, in the Complex water body multi-parameter of the utility model
It is above-mentioned fixed chip used in fast inspection equipment, slidingtype chip also can be used as needed.
As the slidingtype chip, can have reaction tank and/or runner on the substrate, and in the cover plate
It is upper that there is reaction tank and/or runner, by being mutually shifted for the substrate and the cover plate, can make between runner and reaction tank
Connected relation change, such as form two disconnected reaction tanks on substrate, form runner on cover plate, it is mobile before runner
Two reaction tanks cannot be connected to；And being mutually shifted by cover plate and cover plate, runner can be connected to two reaction tanks, so that
Sample and/or preset reagent in two reaction tanks can react to each other, consequently facilitating the progress reacted in control chip.It is described
Can according to need on slidingtype chip and multiple reaction tanks and multiple runners be set, adapt to Complex water body multi-parameter examine fastly in it is various
Complicated, high pass quantitative response demand.Above-mentioned movement can be sliding or rotation etc. in plane.
A preferred embodiment according to the present utility model, as shown in the a-d in Fig. 2, the rotatable chip 1 is wrapped
Include substrate and cover plate, the reaction tank setting on the substrate, is provided with reagent on the cover plate, the substrate and described
Cover plate relative movement, so that the reaction tank is interconnected or separates with the reagent.A in Fig. 2 shows rotatable
The substrate of chip 1 is provided with reaction tank thereon, and b shows the cover plate of rotatable chip 1, is provided with reagent thereon.In Fig. 2
C, d be respectively the rotatable chip 1 two kinds of rotation status figures, show reaction tank and the connection of different reagent
State.
According to the utility model, it further includes sample introduction component that the Complex water body multi-parameter examines equipment fastly, the sample introduction component
For by sample feeding into the chip.There is no particular limitation for the sample introduction component, can complete above-mentioned sample feeding behaviour
Work can use existing any sampling device.From the point of view of convenient for operation and portability, it is preferable that
The sample introduction component is manual injector.
As a kind of manual injector of the utility model, as shown in figure 3, the manual injector 1 include sample cell 12,
Control unit 11 and out sample portion 13, the control unit 11 are connected with the sample cell 12, for entering sample and being maintained at institute
It states in sample cell 12 or sample is made to release the sample cell 12 through the sample portion 13 out, the sample portion 13 out is located at the sample
The side opposite with the control unit 11 in product pond 12；The injection port 23 of the manual injector 1 and chip 2 is tightly connected.
The manual injector 1 can cooperate with the chip 2, realize sample introduction and control sample in the chip 2
Flow process purpose, the manual injector 1 can be used for sampling, thus the dress that the very big operation simplified needs
It sets, is highly convenient for the use of sampling device under various application scenarios.Flow process of the above-mentioned control sample in chip 2, simultaneously
Including the process for pushing sample to move forward or back, so as to so that the function of chip is more diversified, such as sample can be made anti-
Again by some reaction tank to accelerate the mixing of sample, or after first being mixed with the sample in some chamber, then with it is other
The sample of reaction tank mixes.
As needed, the injection port 23 can protrude from the outside of chip, also can be set in the inside of chip, preferably
The injection port 23 is provided with screw thread, to be screwed on sample portion 13 out.
According to the utility model, the manual injector 1 and the connection relationship of the chip 2 can be according to different manual
Sample injector is selected.
A preferred embodiment according to the present utility model, it is complete for the ease of the manual injector 1 is used alone
At sampling process, sample portion 13 and the injection port 23 of chip 2 out of the manual injector 1 are removably tightly connected.Sampling
When, the manual injector 1 can be removed, enter sample by the operation control unit 11 and be maintained at the sample cell
In 12, then going out manual injector 1 after sample portion 13 connect with the injection port 23 of chip 2, the control unit 11 is operated by sample
The injection port that the sample cell 12 enters the chip 11 is released through the sample portion 13 out.
It can be to be screwed on or connected by elastic sealing element as the above-mentioned concrete mode being removably tightly connected
It connects, the bottom in the preferably described sample portion 13 out has helicitic texture or flexible seal member, is preferably screwed on.The helicitic texture can
Think internal thread structure or external thread structure；The flexible seal member can be rubber stopper.
Another preferred embodiment according to the present utility model, in order to be further simplified device, the hand sampling
The sample portion 13 that goes out of device 1 is fixedly connected with the injection port 23 of the chip 2.It is highly preferred that the sample cell of the manual injector 1
12, go out sample portion 13 and the injection port 23 of the chip 2 is formed as one, in use, sample directly can be added in sample cell 12
Product, then the control unit 11 is coupled with sample cell 12, by operation control portion 11, control the flowing of sample in the chips
Process.
A further preferred embodiment according to the present utility model, the control unit 11 control the interior of sample cell 12
The range of chamber (i.e. the chamber for sample storage of sample cell 12 and the formation of control unit 11) volume change is according to the chip 2
Internal structure is selected.As long as the sample of specified amount is packed into sample cell as a result, so that it may which accurate control sample is in chip
Mobility status in 2 each runner, reaction tank facilitates the control that sample flow in experimentation is repeated several times, and improves experiment
Repeatability.
According to the utility model, it is preferable that the control unit 11 and the sample cell 12 are detachably connected.By by institute
It states control unit 11 and the sample cell 12 is detachably connected, the two can be separated, convenient for adding sample in sample cell.
According to the utility model, there is no particular limitation for the shape of the sample cell 12 and the control unit 11, and the two can
To cooperate, realization enters sample and is maintained in the sample cell 12 or sample is made to release institute through the sample portion 13 out
State sample cell 12.Specifically, the sample cell 12 and the control unit 11 are preferably all cylindricality.The control unit 11 and institute
Stating the connection type of sample cell 12, also there is no particular limitation, such as can be connected and sealed, be screwed on by seal member
Deng from the aspect of convenient for operating the control unit 11, the preferably described control unit 11 is screwed on the sample cell 12.
A preferred embodiment according to the present utility model, the process that the control unit 11 controls sample flow pass through
The cavity volume of sample cell 12 caused by relative motion between the control unit 11 and the sample cell 12, which changes, to be completed.
Preferably, the sample cell 12 and control unit 11 can be set to tube-in-tube structure.Wherein, the control unit 11 can be with
As outer cylinder or inner cylinder.It is highly preferred that the inside of the sample cell 12 is arranged in the control unit 11.Specifically, the sample
Product pond 12 can be the outer cylinder of upper opening, and the control unit 11 can be the inner cylinder of sealed bottom.Preferably, the control unit
11 may be set to be telescopic piston.By setting piston for control unit 11, can be relatively moved in sample cell 12.It is logical
Crossing makes control unit 11 and sample cell 12 cooperate, and control unit 11 relatively moves inside sample cell 12, makes the interior of sample cell 12
Chamber size changes, and control sample enters or outflow sample cell 12.
In order to complete control unit 11 and sample cell 12 and be tightly connected, preferably set in the lateral wall bottom of the control unit 11
It is equipped with sealing ring, the sealing ring is for sealing gap between the control unit 11 and the sample cell 12.
Another preferred embodiment according to the present utility model, the process that the control unit 11 controls sample flow are logical
The deformation process for crossing the control unit 11 itself is completed.The control unit 11 have can deformation part, and by this can deformations
The deformation divided changes the volume of 12 inner cavity of sample cell.It is described can deformation part shape and set-up mode do not limit particularly
It is fixed, it is preferable that the control unit 11 can deformations be divided into the scalable side wall of alterable height.It is specifically as follows folding side
Wall, for example formed as longitudinal section be continuous N font tubular or middle part can deformation part connection up and down two can not deformation
Partial structure (wherein, can deformation part can be made of flexible material, by can the deformation of deformation part change two up and down
Can not deformation part mutual alignment relation, such as by horizontal connection structure variation be mutual insert structure) etc..The control unit
11 side wall as can deformations timesharing, preferably deformation occurs at top, and when use can apply pressure or drawing at the top of this
Power manipulates the Sidewall Height variation of the control unit 11.
As above-mentioned control unit 11 can deformation part material, preferably flexible material, more preferably flexible macromolecule
Polymer is specifically as follows one of polyethylene, polypropylene, polytetrafluoroethylene (PTFE), polytetrafluoroethylene (PTFE) or a variety of, wherein being preferably
Polyethylene and/or polypropylene.
The material of the manual injector 1, it is however preferred to have translucency and chemically inert material.For the ease of observe into
Sample process, the preferably described sample cell 12 are transparent or semitransparent sample cell.As the material of the sample cell 12, preferably high score
Sub- polymer is specifically as follows polyethylene, polypropylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene (PTFE), polycarbonate, poly- methyl
One of methyl acrylate, ABS plastic, phenolic resin, epoxy resin, glass and quartz are a variety of, wherein preferably poly- second
One of alkene, polypropylene, polystyrene and polyvinyl chloride are a variety of.In addition, the control unit 11 is except can be in addition to deformation part
Part and it is described go out sample portion 13 it is also preferred that using above-mentioned material.
If Fig. 3 shows, it includes manual injector 1 and chip 2 that the Complex water body multi-parameter of the utility model examines equipment fastly.Fig. 3
Middle g shows the structure of chip 2, and the chip 2 is round dispersion shape multiple flow passages chip, including 1 injection port 23 and 6 with into
22,6 runners 21 of reaction tank that sample mouth 23 is equally spaced are respectively communicated with each reaction tank 22 and injection port 23；In Fig. 3 in a-d
The injection port 23 of chip 2 is illustrated only, the one group of runner 21 and reaction tank 22 of chip 2 are illustrated only in e-f；The hand sampling
The sample cell 12 of tubular of device 1 including polyethylene, the control unit 11 of cupuliform and sample portion 13, the control unit 11 are arranged in institute out
The inside of sample cell 12 is stated, and is removably tightly connected with the sample cell 12, for entering sample and being maintained at described
In sample cell 12 or sample is made to release the sample cell 12, and the lateral wall bottom of the control 11 through the sample portion 13 out
Portion is provided with the sealing ring of rubber system, and sample portion 13 is located at the bottom of the sample cell 12 out；The manual injector 1 and the core
The injection port 23 of piece 2 is formed as one.Fluid sample is added in sample cell 12 first when sample introduction, control unit 11 is set
In in sample cell 12, the sealing ring is contacted with the inner sidewall of sample cell 12, pushes control unit 11 until touching interface bottom.
Sample enters under pressure in the runner 21 of chip 2, and sample flow rate is gradually reduced in flow process, in a period of time
Liquid fills up first reaction tank after (such as 10-60s), and liquid is released reaction tank since 23 end pressure of injection port at this time is less than
22 enter the pressure in gap between two layers of chip, and sample will not continue to overflow, all reactions after a period of time (such as 10-60s)
Pond is filled, and sample stops flowing.
A preferred embodiment according to the present utility model, as shown in figure 4, the sample introduction component can also include pre-
Processing unit 14, the pretreatment portion 14 is for pre-processing sample.The sample portion 13 out is located at the pretreatment portion 14
The side opposite with control unit 11 is pressed again after the processing for making sample pass through pretreatment portion by the movement of the control unit 11
The manual injector out.
According to the utility model, for a variety of pretreatment operations for cooperating sample to need, the preferred packet of the pretreatment portion 14
1 or more preprocessing module (14a, 14b, 14c) is included, preferably 2 or more, more preferably 2-5 is a, further preferably 2-
3.
As the pretreatment operation carried out in each preprocessing module, can be selected according to the sample of institute's sample introduction.Tool
Body, the preprocessing module in filtering module, enrichment module, separation module, pH adjustment module and the reaction module one
Kind is a variety of.As filtering module, wherein fiber, porous solid, accumulation medium etc. can be set, such as polypropylene is fine
One of dimension, glass fibre, active carbon, gravel are a variety of, wherein preferably polypropylene fibre, glass fibre；As enrichment
Module, wherein aluminium oxide, silica, targeting vector can be set；As reaction module, wherein may include the reaction needed
Object, such as the mercuric sulfate with chloride progress precipitation reaction.For example, when carrying out the pretreatment of phosphate from sewage measurement sample,
The preprocessing module includes filtering module, pH adjustment module and pre-reaction module；Carry out polycyclic aromatic hydrocarbon measurement sample in water
When pretreatment, the preprocessing module includes filtering module and enrichment module.
According to the utility model, pass through detachably connected, the Ke Yifang by the pretreatment portion 14 and the sample cell 12
Different preprocessing modules is just set, so that manual injector be made to meet the needs of different sample pretreatments and sample introduction.As can
The mode releasably connected, the sample cell 12 can be detachably connected described pre- by plug division, screw thread, buckle or groove
Processing unit 14.
In order to improve the leakproofness of sample cell 12 and pretreatment portion 14, such as can be in sample cell 12 and pretreatment portion 14
Sealing performance between interconnecting piece can according to need between further setting sealing accessory, such as gasket
Piece etc. guarantees that carry out process is gone on smoothly to improve the leakproofness of sample injector.
As shown in figure 4, the manual injector used includes control unit 11, sample cell 12, pretreatment portion 14 and sample portion out
13, control unit 11, sample cell 12 and pretreatment portion 14 are cylindrical, transparent glass material.The pretreatment portion 14 was
Filter module.The filtering module is internally provided with polypropylene fibre filter disc；Sample inlet is arranged in its top, in the side of the sample inlet
Internal screw thread is provided on wall.Sample export is set in the bottom of sample cell 12, outer spiral shell is provided on the side wall of the sample export
The sample inlet of pretreatment portion 14 and the sample export of sample cell 12 are screwed on by line, keep pretreatment portion 14 and sample cell 12 removable
It is tightly connected with unloading.The sample export of pretreatment portion 14 is as sample portion 13 is gone out, and sample portion 13 is located in 14 bottom of pretreatment portion out
Between portion, the external screw thread for being screwed on micro-total analysis chip is provided on side wall.Control unit 11 can be with 12 shape of sealed sample pond
At sample cavity, make the mobile volume that can change sample cavity of control unit 11 by pushing manually, and pass through the sample in sample cavity
It crosses pretreatment portion 14 and is extruded by going out sample portion 13.Before starting sample introduction, control unit 11 is opened, sample is injected into sample injector, closed
Control unit 11 is closed, the injection port of the sample injector and chip is screwed on, control unit 11 is then pushed to make the smaller volume of sample cavity, sample
Product enter chip after the filtering of the filter disc of pretreatment portion 14.Observation enters the water sample of chip, has no grainless impurity.
According to the utility model, the detection device includes more than one detection part.The detection part is for detecting
The signal of sample on chip.
In the present invention, there is no particular limitation for the detection part, can be used for the various detections of chip detection
Component, such as the detection part can be chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman
One of signal detecting part and infrared spectroscopy detection part are a variety of.It is wherein preferably absorbance detection component and fluorescence inspection
Survey one of component or a variety of.
A preferred embodiment according to the present utility model, as shown in figure 5, the detection part by light source 31, point
Road optical fiber 32 and spectroscopy equipment 33 are constituted.The light source 31 is for generating the light for carrying out testing required wavelength, the branch optical fiber
32 are used for while detecting optical signalling in multiple reaction tanks, and the spectroscopy equipment 33 is for collecting the letter of the light after reaction tank
Number.When being provided on chip with the centre distance of the chip not equal more than two reaction tanks, the branch optical fiber 32 can
The incident light for being used to detect is divided into multichannel, to realize the detection for the reaction tank not waited with the centre distance of the chip.
According to the utility model, in order to realize the effects of data processing, data are transmitted, the Complex water body multi-parameter is examined fastly
Equipment further includes the mechanical part that can also include drive chip rotation, signal acquisition component, data processor, data output
One of component, data transfer component are a variety of.Examine a kind of preferred embodiment of equipment fastly as Complex water body multi-parameter,
It further includes data processor, data output component, data transfer component.As the data processor, such as can be with
It is the central processing unit etc. for including controller, arithmetic unit and register；It may include that there is figure as the data output component
Display screen, touch screen, voice output of shape output function etc.；As the data transfer component, initial data can be uploaded
It is remotely handled to remote server, then result is returned directly into designated terminal, data processor can also be generated
Treated, and information remote transmitting to cloud carries out information collection.
The detection of many kinds of parameters is realized, the Complex water body is more according to the utility model in order to cooperate the markd chip of tool
Parameter is examined fastly has identification component and more than one test section in equipment, the identification part is used on the chip for detecting
Determine that the label of detection signal, the test section are used to determine that the detection label of signal to be corresponding on the chip.In core
In the rotary course of piece, the identification part identifies the label that each reaction tank setting is corresponded on the chip, then determines respectively every
A reaction tank needs the detection mode used, to realize the detection respectively to unlike signal object.
The utility model second aspect provides a kind of fast detecting method of Complex water body multi-parameter, utilizes above-mentioned the utility model
Complex water body multi-parameter is examined equipment fastly and is detected, and by sample feeding into the chip, and is carried out by the detection device
Detection.
It, in the present invention, can be by rotating the chip or making institute in order to which the detection of multi-parameter is rapidly completed
State the sample detection that chip is mobile relative to the detection device, in the different reaction tanks of completion.It, can for rotary chip
To complete to detect by rotary chip；For straight-line chip, can complete to detect by pull chip.
A preferred embodiment according to the present utility model, by being arranged on the chip for determining detection letter
Number label, determine the detection part that detection device uses.By with the use of the markd chip of tool, the side of the utility model
Method can be in the detection process of short time processing multiple types sample signal, to improve detection speed.
The index that the method for the utility model can detecte includes COD, total nitrogen, sulfide, total phosphorus etc., specifically be can be used
Corresponding reagent detects after handling sample., can be preset in the chips by reagent for the reaction for needing to be added reagent,
To improve detection accuracy, and easily facilitate operation.
The method of the utility model can be filled according to the needs chip suitably selected to use, detection part, sample introduction of detection
It sets, to be attained by good detection effect for various samples, appropriate pre-process, instead can also be set as needed
Process is answered, detection is more quickly and easily completed.It is especially suitable for being detected for water sample.
The utility model will be described in detail by embodiment below.In following embodiment, specific detection agents
Respectively Japan's commercially available product that vertical physical and chemical company's trade mark is WAK altogether, sample COD, total nitrogen, sulfide, total phosphorus titer mix
Close solution.
Embodiment 1
It includes manual injector 1, chip 2 and such as Fig. 3 institute that Complex water body multi-parameter used in the present embodiment examines equipment fastly
The built-in hand-held spectrophotometer of rotary fixing frame shown.
Sample introduction is carried out using manual injector 1 as shown in Figure 3；Using band, there are four types of the circles of not isolabeling to have label
Rotary chip, all 12 reaction tanks be averaged scattering device on the circumference of the center of circle equidistance R away from chip (in Fig. 3
12 reaction tanks are set on the basis of middle g signal).The chip 2 is round dispersion shape multiple flow passages chip, including 1 injection port
23 and 12 reaction tanks being equally spaced with injection port 23,22,12 runners 21 are respectively communicated with each reaction tank 22 and injection port
23；The injection port 23 of chip 2 is illustrated only in Fig. 3 in a-d, the one group of runner 21 and reaction tank of chip 2 are illustrated only in e-f
22；
The sample cell 12 of tubular of the manual injector 1 including polyethylene, the control unit 11 of cupuliform and sample portion out
13, the inside of the sample cell 12 is arranged in the control unit 11, and is removably tightly connected with the sample cell 12, uses
In enter sample and be maintained in the sample cell 12 or make sample through it is described go out sample portion 13 release the sample cell 12, and
And the lateral wall bottom of the control 11 is provided with the sealing ring of rubber system, sample portion 13 is located at the bottom of the sample cell 2 out；
The injection port 23 of the manual injector 1 and the chip 2 is formed as one.
Fluid sample is added in sample cell 12 first, control unit 11 is placed in sample cell 12, the sealing ring and sample
The inner sidewall in product pond 12 contacts, and pushes control unit 11 until touching interface bottom.Sample enters chip 2 under pressure
In runner 21, sample flow rate is gradually reduced in flow process, and liquid fills up first reaction tank after 32s, due at this time
23 end pressure of injection port, which is less than, releases the pressure that reaction tank 22 enters gap between two layers of chip for liquid, and sample will not continue to overflow
Out, all reaction tanks are filled after 19s, and sample stops flowing.
The hand-held spectrophotometer of the built-in rotary fixing frame is by light source 31, branch optical fiber 32 and spectroscopy equipment 33
It constitutes, and it is (total for optional 410nm (total nitrogen), 620nm (COD), 665nm (sulfide), 880nm to be prefixed measurement wavelength
Phosphorus).Hand rotary process is used when test, rotates chip around the center of circle.Specific detection examination is prefixed on it can recognize chip
Agent reacts with preset reaction reagent after sample solution is introduced reaction tank, absorption values is read after 30min.
1-3 is total nitrogen detection cell in 12 reaction tanks, and 4-6 is COD detection cell, and 7-9 is sulfide detection cell, and 10-12 is
Total phosphorus detection cell corresponds to reaction tank in 4 groups and different labels is respectively set.It can be manually rotated chip and pass through above-mentioned reaction tank one by one,
Spectrophotometer determines that the corresponding measurement wavelength of index to be detected, the wavelength for then changing light source are gone forward side by side after detecting above-mentioned label
Row measurement.The corresponding concentration measured is respectively such as the following table 1.
Table 1
Embodiment 2
It includes round recognizable chip and as shown in Figure 3 that Complex water body multi-parameter used in the present embodiment examines equipment fastly
The built-in hand-held spectrophotometer of rotary fixing frame.
Using band, there are four types of the circles of not isolabeling can recognize that chip, 4 reaction tanks are averaged scattering device away from chip
The distance in the center of circle is the circle that distance of the scattering device in the center of circle away from chip is R2 in addition, 4 reaction tanks are averaged on the circumference of R1
Zhou Shang.The hand-held spectrophotometer of this is built-in rotary fixing frame is made of light source 31, branch optical fiber 32 and spectroscopy equipment 33,
And being prefixed measurement wavelength is optional 410nm (total nitrogen), 620nm (COD), 665nm (sulfide), 880nm (total phosphorus).It surveys
Hand rotary process is used when examination, rotates chip around the center of circle.Specific detection agents are prefixed on it can recognize chip, are being incited somebody to action
After sample solution introduces reaction tank, reacts with preset reaction reagent, absorption values are read after 30min.
It 1,2 is total nitrogen detection cell that the distance in the center of circle away from chip, which is on the circumference of R1, in 8 reaction tanks, and 3,4 examine for COD
Survey pond, it 5,6 is sulfide detection cell that distance, which is on the circumference of R2,7,8 is total phosphorus detection cell.Reaction tank is corresponded in 4 groups to set respectively
Set different labels.Can be manually rotated chip and pass through above-mentioned reaction tank one by one, spectrophotometer detect determine after above-mentioned label to
The corresponding measurement wavelength of Testing index, is then measured.The corresponding concentration measured is respectively such as the following table 2:
Table 2
Preferred embodiments of the present invention are described in detail above, still, the utility model is not limited to this.At this
In the range of the technology design of utility model, a variety of simple variants can be carried out to the technical solution of the utility model, including each
Technical characteristic is combined with any other suitable method, these simple variants and combination equally should be considered as the utility model
Disclosure of that belongs to the protection scope of the utility model.
Claims (10)
1. a kind of Complex water body multi-parameter based on microflow control technique examines equipment fastly, which is characterized in that the Complex water body multi-parameter
Fast inspection equipment includes chip and detection device；
Wherein, the chip is rotary chip or straight-line chip, and the chip includes the reaction tank for example reaction；
The detection device is used to detect the signal of the sample in the reaction tank.
2. Complex water body multi-parameter according to claim 1 examines equipment fastly, which is characterized in that be arranged on the chip useful
In the label for determining detection signal, the label is correspondingly arranged with the reaction tank.
3. Complex water body multi-parameter according to claim 1 examines equipment fastly, which is characterized in that the institute of the rotary chip
Multiple reaction tanks are stated to be arranged in using the center of the chip as on the circumference in the center of circle.
4. Complex water body multi-parameter according to claim 1 examines equipment fastly, which is characterized in that the chip include substrate and
Cover plate, the substrate are fixedly connected with the cover plate, alternatively, the substrate and the cover plate can be mutually shifted, to change
Connected relation in the chip between reaction tank and/or runner.
5. Complex water body multi-parameter described in any one of -4 examines equipment fastly according to claim 1, which is characterized in that described multiple
It further includes sample introduction component that miscellaneous water body multi-parameter examines equipment fastly, and the sample introduction component is used for sample feeding into the chip.
6. Complex water body multi-parameter according to claim 5 examines equipment fastly, which is characterized in that the sample introduction component is manual
Sample injector.
7. Complex water body multi-parameter according to claim 6 examines equipment fastly, which is characterized in that the sample introduction component includes pre-
Processing unit, the pretreatment portion is for pre-processing sample.
8. Complex water body multi-parameter described in any one of -4 examines equipment fastly according to claim 1, which is characterized in that the inspection
Surveying device includes more than one detection part.
9. Complex water body multi-parameter according to claim 8 examines equipment fastly, which is characterized in that the detection part is coloration
In detection part, absorbance detection component, fluorescence signal detection part, Raman signal detection component and infrared spectroscopy detection part
It is one or more.
10. Complex water body multi-parameter described in any one of -4 examines equipment fastly according to claim 1, which is characterized in that described
Complex water body multi-parameter examine fastly equipment further include drive chip rotation driving part, signal acquisition component, data processor,
One of data output component and data transfer component are a variety of.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408068A (en) * | 2019-06-25 | 2019-11-05 | 中国科学院上海微系统与信息技术研究所 | A kind of mandruka umbrella-shaped structure PDMS chip of doping fluorescent dyestuff and preparation method thereof and application |
| CN110470609A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Complex water body multi-parameter based on microflow control technique examines device and method fastly |
| CN112903793A (en) * | 2021-01-21 | 2021-06-04 | 中国科学技术大学 | Multi-parameter rapid detection device and method for water body |
-
2018
- 2018-05-11 CN CN201820700728.9U patent/CN208366832U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110470609A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Complex water body multi-parameter based on microflow control technique examines device and method fastly |
| CN110408068A (en) * | 2019-06-25 | 2019-11-05 | 中国科学院上海微系统与信息技术研究所 | A kind of mandruka umbrella-shaped structure PDMS chip of doping fluorescent dyestuff and preparation method thereof and application |
| CN112903793A (en) * | 2021-01-21 | 2021-06-04 | 中国科学技术大学 | Multi-parameter rapid detection device and method for water body |
| CN112903793B (en) * | 2021-01-21 | 2022-04-19 | 中国科学技术大学 | Multi-parameter rapid detection device and method for water body |
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Effective date of registration: 20221118 Address after: Yanan City, Shandong province Qingdao City three road 266071 No. 218 Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec Safety Engineering Research Institute Co.,Ltd. Address before: Yanan City, Shandong province Qingdao City three road 266071 No. 218 Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: SINOPEC Research Institute OF SAFETY ENGINEERING |