CN105969651A - Microorganism oscillating cleaning and recycling system - Google Patents
Microorganism oscillating cleaning and recycling system Download PDFInfo
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- CN105969651A CN105969651A CN201610425926.4A CN201610425926A CN105969651A CN 105969651 A CN105969651 A CN 105969651A CN 201610425926 A CN201610425926 A CN 201610425926A CN 105969651 A CN105969651 A CN 105969651A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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Abstract
The invention relates to the technical field of medical instrument machining, in particular to a microorganism oscillating cleaning and recycling system. The microorganism oscillating cleaning and recycling system comprises a tank, a sample inlet, a liquid inlet and an exhaust hole, wherein the sample inlet, the liquid inlet and the exhaust hole are formed in the tank. A waste liquid outflow channel, a gas inlet/outlet and a sample outlet are formed in the lower portion of the tank. A first filter membrane is arranged on the lower portion in the tank. Holding spaces connected with an oscillator are further arranged on the outer wall of the tank. By means of the system, needed microorganisms (florae) can be conveniently separated out from an original liquid-phase medium mixed with toxic and harmful substances, and the microorganisms (florae) are mixed with a new buffer solution of a specific volume to form a biological sample capable of being directly applied. Meanwhile, all processes are completed in the relatively airtight system, so that various defects of an existing centrifugation-related purification scheme are effectively overcome.
Description
Technical field
The present invention relates to the technical field of medical apparatus and instruments processing, more particularly, to a kind of microorganism concussion clean and reuse system.
Background technology
In human body intestinal canal, a large amount of microorganism in field planting, and classification is more than 2000 kinds, and overall quantity is approximately about 10 times of human body cell sum, and weight is up to 1.5kg.The summation of intestinal microbial population genomic information is referred to as grand genome.It is the mankind's the second genome controlling health, body self new city metabolism with human body autogene group joint effect.Human body more can be seen as " the superior biological body " collectively formed by the microbiologic population of human body eukaryotic cell and parachorium, there is the interaction process of complexity, reciprocal causation, affect survival condition each other between human body and its interior flora.Substantial amounts of research discloses the composition by regulating intestinal canal flora, it is possible to achieve the control to multiple disease is even treated, thus facilitates operating system to there is demand these mixed cells of acquisition clinically.Antibacterial in the natural mixtures such as feces is directly recycled the Enrichment of bacteria mode being to be most frequently with.
During enrichment of bacterial, the most farthest realize high-efficient cleaning except harmful components in mixture while, realize again bacterial community is constituted than, sum and the protection of vigor, be the sport technique segment of non-the normally off key.
For the microorganism in clean and reuse mixture (soil, sea water, feces etc.), existing transit scenario is mainly completed by artificial transfer techniques based on centrifuge.Because centrifuge is one of all laboratory basic configuration, the method is widely adopted.But its shortcoming is apparent in application process: 1. need manual operation；2. needing repeatedly centrifugally operated, the longest: the microbe species distributed number in nature mixture is uneven, the total amount and activity for guaranteeing antibacterial in sample generally requires and processes bulk sample by several times, wastes time and energy；3. relating to centrifuge because of operating process, between variations in temperature that centrifugal process causes, rapid subsidence, thalline, the physical action such as extruding can damage bacterial action, and this degree of injury can increase the weight of with the prolongation of centrifugal speed and time.Need optimized choice operational factor (temperature, rotating speed, centrifugal duration), to guarantee to be operated the vigor of object；4., for adapting to centrifuge operation logic, sample often needs subpackage, thus in sample, microbiologic population has by the risk of environment bacterium secondary pollution, is also possible to contamination (microorganism pollution, odor contamination etc.) peripheral environment simultaneously.
Summary of the invention
The present invention is to overcome at least one defect described in above-mentioned prior art, it is provided that a kind of microorganism concussion clean and reuse system, simplifies the clean and reuse process of microorganism in mixture (soil, sea water, feces etc.).Reduce artificial participation; shorten operation duration, drop oligosaprobic risk, can shake because of microorganism to be recycled simultaneously always and be suspended in appointment liquid environment; bacterial action also available farthest protection, specific aim compensate for the existing deficiency relating to centrifuge operations scheme.
For solving above-mentioned technical problem, the technical solution used in the present invention is: a kind of microorganism concussion clean and reuse system, wherein, including tank body, be located at the injection port on tank body top, inlet, steam vent, described tank body lower part be provided with waste liquor stream engage in this profession, entry/exit QI KOU, outlet；
The described bottom in tank body is provided with the first filter membrane, and tank wall is additionally provided with the gripping space being connected with agitator.
In same closed system, utilizing membrane filtration and shake resuspended technology, the present invention can carry out Rapid Cleaning and the preparation of subsequent use system to microorganism in mixture, enormously simplify operating procedure；Medical disposable material can be used because of main body of the present invention, it is not necessary to recovery and rinsing processes, and above-mentioned steps is all conducive to shortening the operating time simultaneously.
Because eliminating the process using centrifuge, decrease the artificial participation of intermediate link；
The clean and reuse process of microorganism in mixture is all completed in the same space, either to mixed microorganism group still to periphery spatial environments, all reduces pollution risk；
System is provided with specific inlet, entry/exit QI KOU, it is beneficial to the application demand according to flora, control the gaseous environment (such as detesting/hypoxia, normal oxygen etc.) when it cleans, its resuspended environment of final liquid can be adjusted simultaneously, be the buffer such as normal saline as replaced resuspension fluids；
On the one hand the first filter membrane at waste liquid outlet can at utmost retain antibacterial in liquid phase by filtration, filters little granule or the harmful components such as unnecessary virus；Simultaneously can be through valve with the help of vacuum suction or inversely filling into gas, poor by controlling liquid in-out rate of flow of fluid, make antibacterial to be collected be in all the time in the liquid environment of certain volume, keep activity, additionally can also utilize the outflow process that can interrupt at any time, obtain quantitative antibacterial suspension at end.
Further, it is provided with adsorbent layer in described steam vent.Because the strong absorbability of the adsorbents such as activated carbon can avoid the distinctive odor contamination of antibacterial mixed liquor.
Further, the second filter membrane it is additionally provided with in described steam vent.Can prevent antibacterial inside and outside tank from passing in and out the Bacterial cross caused with gas.
Further, described injection port is in the vertical top inserting tank body, and inlet tilts to be located at the sidepiece of injection port.This should arrange convenient input mixed liquor and the novel solvent (such as normal saline) of designated volume.
Further, described steam vent is in the vertical top inserting tank body.Too much gas will be discharged by the steam vent at tank body top.
Further, described tank body includes cylinder-like structure, is located at cylinder-like structure bottom and the funnel-shaped structure of both connections.The first described filter membrane is located between cylinder-like structure and funnel-shaped structure.
In the present invention, the design of cylinder-like structure and funnel-shaped structure facilitates the circulation of liquid.
Further, described waste liquor stream is engaged in this profession and is located at the bottom of funnel-shaped structure and connected, and entry/exit QI KOU tilts to be located at the sidepiece that waste liquor stream is engaged in this profession.The arranging of said structure facilitates waste liquid to flow out and the gas (such as anaerobism gaseous mixture) specified can enter system with constant flow rate.
Further, described the first filter membrane, the aperture of the second filter membrane are 0.2-0.5 micron.
Compared with prior art, provide the benefit that: utilize native system the microorganism (flora) of needs to be mixed with the liquid phase medium of poisonous and harmful substance from originally easily and separate, and mix with the new buffer of designated volume, composition can direct applied biological specimen；Because objective microbe (flora) is suspended in liquid environment with the help of external force is shaken always, the available maximum protection of thalline vigor.Meanwhile, because all processes all complete in the most airtight system, effectively avoid the shortcomings of existing centrifugal relevant purification scheme.Native system can be applicable to multiple fields such as separation inspection and the microbial components analysis of microorganism in a large amount of sea water, soil, diet and feces.
When applying this technology in caprophyl preparation field, the feces homogenizing bag (authorized utility model patent, the patent No.: ZL201520585188.0 in person) being resistant to pat can be used in series.
By having the liquid of flora to be analyzed, solids mixing sample, it is encased in homogenizing bag, fully homogenizes；Subsequently according to the difference of application, the membrane filtration gained liquid through 5mm to 0.1mm will be injected native system, under given conditions, such as anaerobic condition, in the enclosed environment of native system, be rapidly completed the replacement of liquid suspension environment, obtain target bacteria suspension.
Accompanying drawing explanation
Fig. 1 is the embodiment of the present invention 1 overall structure schematic diagram.
Fig. 2 is the embodiment of the present invention 2 overall structure schematic diagram.
Fig. 3 is the embodiment of the present invention 2 filter course schematic diagram.
Detailed description of the invention
Accompanying drawing being merely cited for property explanation, it is impossible to be interpreted as the restriction to this patent；In order to the present embodiment is more preferably described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product；To those skilled in the art, in accompanying drawing, some known features and explanation thereof may will be understood by omission.Being merely cited for property of position relationship explanation described in accompanying drawing, it is impossible to be interpreted as the restriction to this patent.
Embodiment 1
As it is shown in figure 1, a kind of microorganism concussion clean and reuse system, wherein, including tank body 11, be located at the injection port 1 on tank body 11 top, inlet 2, steam vent 8, tank body 11 bottom be provided with waste liquor stream engage in this profession 4, entry/exit QI KOU 5, outlet 9；
Bottom in tank body 11 is provided with the first filter membrane 3, and tank body 11 outer wall is additionally provided with the gripping space 10 being connected with agitator.
Present invention aim at the clean and reuse process of microorganism in mixture (soil, sea water, feces etc.) is optimized, can be rapidly completed in a space the most airtight, the gaseous environment condition (such as detesting/hypoxia, normal oxygen etc.) that simultaneously can need for objective microbe, the liquid phase specifying system purifies.
In the present embodiment, in steam vent 8, it is provided with adsorbent layer 7.The second filter membrane 6 it is additionally provided with in steam vent 8.Because the strong absorbability of the adsorbents such as activated carbon can avoid the distinctive odor contamination of antibacterial mixed liquor.Can prevent antibacterial inside and outside tank from passing in and out the Bacterial cross caused with gas.
Tank body 11 includes cylinder-like structure 111, is located at cylinder-like structure 111 bottom and the funnel-shaped structure 112 of both connections.First filter membrane 3 is located between cylinder-like structure 111 and funnel-shaped structure 112.In the present embodiment, the design of cylinder-like structure 111 and funnel-shaped structure 112 facilitates the circulation of liquid.
In Fig. 1, valve 12 it is additionally provided with at entry/exit QI KOU 5, can be through valve with the help of vacuum suction or inversely filling into gas, poor by controlling liquid in-out rate of flow of fluid, antibacterial to be collected is made to be in all the time in the liquid environment of certain volume, keep activity, additionally can also utilize the outflow process that can interrupt at any time, obtain quantitative antibacterial suspension at end.
Concrete principle is as follows:
Main body is plastics (or being other the disposable reusable materials) tank body being fixed on concussion arm.Sample introduction 1/ inlet 2, steam vent 8 are arranged at top；Gripping space 10 has been reserved to agitator in centre；Bottom is designed with waste liquor stream and engages in this profession 4, entry/exit QI KOU 5 and outlet 9.Mixed liquor containing desired microorganisms, by, in injection port import system, completing the isolation of purified of liquid phase in the system that this is the most airtight, finally can be resuspended in the specific solvent of certain volume and discharge in outlet 9.During whole, after mixed liquor import system, would first, through the first filter membrane 3 of tank body middle and lower part, under the conditions of vacuum suction or reverse gas fill into, the required solid-phase component such as antibacterial in mixed liquor is made to will be left in filter membrane surface, or sustained suspension is in the liquid environment of certain volume, liquid phase is engaged in this profession 4 outflows via waste liquor stream.Stopping vacuum suction subsequently, the novel solvent (such as normal saline) of designated volume will be entered by the inlet 2 on tank body top, under the vibrations of earthquake arm mediation, and the solids phase residue on resuspended filter membrane.Meanwhile, for create antibacterial need gaseous environment and avoid new liquid phase through filter membrane flow out, it is intended that gas (such as anaerobism gaseous mixture) will by tank body lower part entry/exit QI KOU 5 with constant flow rate enter system.For constant pressure in maintenance tank, too much gas will be discharged by the steam vent 8 at tank body top.It is to be noted and gas efferent tract is provided with the second filter membrane 6 and adsorbent layer 7, on the one hand can prevent antibacterial inside and outside tank from passing in and out the Bacterial cross caused with gas；The distinctive odor contamination of antibacterial mixed liquor can be avoided because of the strong absorbability of the adsorbents such as activated carbon simultaneously.If necessary, the resuspended repeatable above-mentioned solid liquid phase of mixed liquor is filtered separation, concussion resuspension process, promotes degree of purification further.Final mixed liquor can directly collect application after outlet 9 flows out.In system, the aperture of filter membrane is 0.2-0.5 micron.
In Fig. 1, the first filter membrane 3 practical structures is as shown in Figure 3.
Embodiment 2
As shown in Figure 2,3, classified filtering system structure explanation: 1A. injection port；2A. filter course [structure is disassembled for a. filter holder, b. filter membrane (aperture may select)]；3A. liquid efferent tract；4A. gas outlet (vacuum suction) the 5 degerming filter membrane of A. (0.22 μm)；The 7 A. gas overflowing roads such as 6 A. adsorbents/activated carbon.
The size of nature overwhelming majority antibacterial is in 0.5-5 μm, but the impurity size contained in the natural mixture that feces etc. are containing bacterium can fluctuate in several centimeters to a few micrometers (can be mixed with the materials such as the dietary fiber of inabundant digestion, worm's ovum in such as feces).By serial graded filtration system before apparatus of the present invention (accompanying drawing 2), we can control to clean the final purity of antibacterial to be separated in piece-rate system.In the classified filtering system of series connection, the pore size scope of filter membrane is 8mm-8 μm, and the concrete number of plies that filters can select at 1-10 interlayer according to the severity of mixing up of sample and cumulative volume.Native system can be single use, it is possible to realize recycling by changing filter course filter membrane.
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.All any amendment, equivalent and improvement etc. made within the spirit and principles in the present invention, within should be included in the protection domain of the claims in the present invention.
Claims (9)
1. a microorganism concussion clean and reuse system, it is characterized in that, including tank body (11), be located at the injection port (1) on tank body (11) top, inlet (2), steam vent (8), described tank body (11) bottom be provided with waste liquor stream engage in this profession (4), entry/exit QI KOU (5), outlet (9)；
The described bottom in tank body (11) is provided with the first filter membrane (3), and tank body (11) outer wall is additionally provided with the gripping space (10) being connected with agitator.
A kind of microorganism concussion clean and reuse system the most according to claim 1, it is characterised in that: it is provided with adsorbent layer (7) in described steam vent (8).
A kind of microorganism concussion clean and reuse system the most according to claim 2, it is characterised in that: it is additionally provided with the second filter membrane (6) in described steam vent (8).
A kind of microorganism concussion clean and reuse system the most according to claim 3, it is characterised in that: described injection port (1) is in vertically inserting the top of tank body (11), and inlet (2) tilts to be located at the sidepiece of injection port (1).
A kind of microorganism concussion clean and reuse system the most according to claim 4, it is characterised in that: described steam vent (8) is in the top vertically inserting tank body (11).
A kind of microorganism concussion clean and reuse system the most according to claim 1, it is characterised in that: described tank body (11) includes cylinder-like structure (111), is located at cylinder-like structure (111) bottom and the funnel-shaped structure (112) of both connections.
A kind of microorganism concussion clean and reuse system the most according to claim 6, it is characterised in that: described the first filter membrane (3) is located between cylinder-like structure (111) and funnel-shaped structure (112).
A kind of microorganism concussion clean and reuse system the most according to claim 6, it is characterized in that: described waste liquor stream engage in this profession (4) be located at the bottom of funnel-shaped structure (112) and connected, entry/exit QI KOU (5) tilts to be located at waste liquor stream and engages in this profession the sidepiece of (4).
A kind of microorganism concussion clean and reuse system the most according to claim 3, it is characterised in that: described the first filter membrane (3), the aperture of the second filter membrane (6) they are 0.2-0.5 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610425926.4A CN105969651A (en) | 2016-06-16 | 2016-06-16 | Microorganism oscillating cleaning and recycling system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610425926.4A CN105969651A (en) | 2016-06-16 | 2016-06-16 | Microorganism oscillating cleaning and recycling system |
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| CN105969651A true CN105969651A (en) | 2016-09-28 |
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| CN201610425926.4A Pending CN105969651A (en) | 2016-06-16 | 2016-06-16 | Microorganism oscillating cleaning and recycling system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034127A (en) * | 2017-06-15 | 2017-08-11 | 黄捷 | A kind of test tube and method for being used to extract microbial gene and Inorganic chemical substance in soil |
| CN107164222A (en) * | 2017-06-22 | 2017-09-15 | 成都远洋蓝猫科技有限公司 | Sample process box for Bacteria Detection |
| CN107287090A (en) * | 2017-06-22 | 2017-10-24 | 成都远洋蓝猫科技有限公司 | Sample preprocessing container |
| CN110220746A (en) * | 2019-07-22 | 2019-09-10 | 茅台学院 | A kind of field planktonic organism quantitative collection bag and application method |
| CN111487107A (en) * | 2020-06-07 | 2020-08-04 | 黑龙江省农业科学院农产品质量安全研究所 | A sample grading plant for food safety inspection |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107034127A (en) * | 2017-06-15 | 2017-08-11 | 黄捷 | A kind of test tube and method for being used to extract microbial gene and Inorganic chemical substance in soil |
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| CN111487107A (en) * | 2020-06-07 | 2020-08-04 | 黑龙江省农业科学院农产品质量安全研究所 | A sample grading plant for food safety inspection |
| CN111487107B (en) * | 2020-06-07 | 2022-12-09 | 黑龙江省农业科学院农产品质量安全研究所 | A sample grading plant for food safety inspection |
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