CN110220746A - A kind of field planktonic organism quantitative collection bag and application method - Google Patents
A kind of field planktonic organism quantitative collection bag and application method Download PDFInfo
- Publication number
- CN110220746A CN110220746A CN201910659479.2A CN201910659479A CN110220746A CN 110220746 A CN110220746 A CN 110220746A CN 201910659479 A CN201910659479 A CN 201910659479A CN 110220746 A CN110220746 A CN 110220746A
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- Prior art keywords
- bag body
- sample
- field
- planktonic organism
- concentration tube
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 230000001376 precipitating effect Effects 0.000 claims abstract description 7
- 238000005070 sampling Methods 0.000 claims abstract description 6
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000009194 climbing Effects 0.000 claims description 3
- 230000009514 concussion Effects 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of field planktonic organism quantitative collection bag and application methods, the collection bag includes bag body and the closing device that bag body oral area is arranged in, the oral area of the bag body is necking, tongs are installed at necking, the concentration tube being connected to bag body inner cavity is installed on bag body top, filtering mesh screen is installed in the junction of concentration tube and bag body, air vent is installed in bag body lower part.Compared with traditional quantitative sampling, sample transport, precipitating, concentration and separator, while the present invention is easy to carry, the space utilization rate for greatly improving sampling vehicle overcomes sample secondary transferring, the phytoplankton of precipitating is easy the long problem of disturbed disadvantage and lock out operation time.Simultaneously as bag body is made of acid resisting material, the durability of bag body is high, more efficiently, durable, and can be operated in field, can significantly improve working efficiency.
Description
Technical field
The present invention relates to a kind of field planktonic organism quantitative collection bag and application methods, belong to water ecological environment monitoring technology
Field.
Background technique
Water eutrophication causes the increase of algae bio amount and structure of community composition to change, and then the ecosystem is caused to lose
Surely, immeasurable loss is caused to the national economic development.In recent years, water environment and water resources management department will swim plant in succession
Object monitoring is brought into routine monitoring range, and phytoplankton quantitative analysis is a basic work in Ecology research,
It is also a ring indispensable in phytoplankton monitoring.
The quantitative analysis of phytoplankton is provided according to " inland waters phytoplankton monitoring technology regulation " SL733-2016:
It is 1000~2000mL that phytoplankton, which quantifies sample amount, and the lower water body of Phytoplankton biomass should take the circumstances into consideration to increase and adopt water
It measures to 3000~5000mL, and quantifying sample need to be according to 10%~20% setting Duplicate Samples of acquisition total number of samples；It adopts in field
The quantitative water sample for collecting and fixing is poured into settling vessel after standing 48h in laboratory, is taken out with the siphon pipe with No. 25 bolting silks
Supernatant, and control siphon flow velocity (should staticly settle again if any disturbance)；Remainder 20~25mL sediment is transferred to fixed after siphon
In measuring bottle, several times with a little supernatant eluant container, it is finally settled to 30mL.Since sample is often more, traditional sampling bottle by
In morphology issues, space can not be efficiently used, occupies more space；Meanwhile it is dense using conventional apparatus progress phytoplankton
Division from when, need the sample that will be acquired back to pour into settling vessel, there is certain loss to sample；In addition, precipitating is after 48 hours
It is time-consuming and laborious that siphon is carried out again, and easily causes disturbance precipitating when siphon, needs to precipitate a few hours again.Therefore this method
It takes time and effort, it is inefficient when especially in a short time needing that a large amount of samples are concentrated.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of structures to be simple and convenient to operate, time saving and energy saving, experiment effect
The high field planktonic organism quantitative collection bag of rate and application method, to overcome deficiency in the prior art.
The technical scheme of the present invention is realized as follows: a kind of field planktonic organism quantitative collection bag, including bag body and set
The closing device in bag body oral area is set, the oral area of the bag body is necking, and tongs are equipped at necking, is pacified on bag body top
Equipped with the concentration tube being connected to bag body inner cavity, filtering mesh screen is installed in the junction of concentration tube and bag body, is pacified in bag body lower part
Equipped with air vent.
Further, the closing device includes the hard injection pipe connecting with bag body oral area, in the oral area of hard injection pipe
It is threaded with lid.
Further, the periphery of the bag body is provided with the edge sealing that a circle is connect with bag body, is installed on the top of edge sealing
Strip-shaped hole offers circular hole in the lower central of edge sealing.
Further, graduation mark is provided on the outer wall of the bag body.
Further, the oral area of the concentration tube is provided with plug.
Meanwhile the present invention provides a kind of application method based on above-mentioned field planktonic organism quantitative collection bag, including following
Step:
Step 1: sample acquires: concentration tube and air vent are closed, lid and tongs are opened, it will from quantitative sampler
Sample is injected into bag body from hard injection pipe, tightens lid after the completion of injection, the label paper for finishing writing sample information is attached to bag
On body, that is, complete sample collecting work；
Step 2: sample concentration: it is hung, is made bag body inversion 2 hours or more from the circular hole of bag body with hook or cord, to
After suspended matter precipitating, tongs can be clipped in bag body necking position, decanting zone, which is carried out isolation, prevents Secondary Disturbance；It opens dense
The draw and air vent allow water to flow out from concentration tube, residual sample in sample upper layer are concentrated, until residue 5-10ml；
Step 3: sample collection: opening tongs, after the sample of decanting zone and enrichment region is mixed, lid is opened, by sample
Product import in sample bottle, then liquid is added from hard injection pipe and carries out concussion cleaning to sample sack, and took out out of concentration tube
Filter mesh sieve is rinsed again with a small amount of sample stoste filtering mesh screen, and the liquid after flushing, which is collected into sample bottle, carries out constant volume.
In the above method, when bag body is carried in field, finger is protruded into strip-shaped hole and guides and supports entire bag body, or uses Fastener for climbing mountain
Protrude into strip-shaped hole, bag body is suspended on.
In the above method, in step 2, such as enriched sample, double medium filtration mesh screen can be installed in concentration tube, and
Concentration tube is connect with suction pump, liquid is extracted out, or closes air vent, promotes liquid to flow out with the mode of manual pressure.
By adopting the above-described technical solution, advantages of the present invention are as follows: transport, precipitate with traditional quantitative sampling, sample,
Concentration and separator are compared, and while the present invention is easy to carry, is greatly improved the space utilization rate of sampling vehicle, are overcome sample
Secondary transferring, precipitating phytoplankton be easy disturbed disadvantage and lock out operation time long problem.Simultaneously as bag
Body is made of acid resisting material, and the durability of bag body is high, more efficiently, durable, and can be operated, can significantly be mentioned in field
High working efficiency.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram when present invention is implemented.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The embodiment of the present invention: referring to Fig. 1, a kind of field planktonic organism quantitative collection bag, including bag body 10 and setting
It is necking in the oral area of the closing device of 10 oral area of bag body, the bag body 10, tongs 4 is installed at necking, in bag body 10
Top is equipped with the concentration tube 5 being connected to 10 inner cavity of bag body, is equipped with filtering mesh screen 6 in the junction of concentration tube 5 and bag body 10,
The aperture for filtering mesh screen 6 is less than 10um, is equipped with air vent 11 in 10 lower part of bag body.The closing device includes and 10 mouthfuls of bag body
The hard injection pipe 2 of portion's connection, is connected with lid 1 in the mouth thread of hard injection pipe 2.The periphery of the bag body 10 is provided with
The edge sealing 3 that one circle is connect with bag body 10, is installed with strip-shaped hole 8 on the top of edge sealing 3, offers in the lower central of edge sealing 3
Circular hole 9；Graduation mark 7 is provided on the outer wall of the bag body 10；The oral area of the concentration tube 5 is provided with plug.
Referring to fig. 2, working principle of the present invention is as follows:
1, sample acquires:
Concentration tube 5 and air vent 11 are closed, lid 1 and tongs 4 are opened, infuses sample from hard from quantitative sampler
Enter pipe 2 to be injected into bag body 10, tightens lid 1 after the completion of injection, the label paper for finishing writing sample information is attached in bag body 10, i.e.,
Complete sample collecting work；When bag body 10 is carried in field, finger can be protruded into strip-shaped hole 8 and guide and support entire bag body 10, or
Protruded into strip-shaped hole 8 with Fastener for climbing mountain, bag body 10 is suspended on.
2, sample concentration:
A. it is hung, is made the inversion of bag body 10 2 hours or more, to mainly suspend from the circular hole 9 of bag body 10 with hook or cord
After object has precipitated, tongs 4 can be clipped in 10 necking position of bag body, major precipitation area, which is carried out isolation, prevents Secondary Disturbance；
B. concentration tube 5 and air vent 11 are opened, allows water to flow out from concentration tube 5, residual sample in sample upper layer is concentrated, until residue 5-
10ml；Such as enriched sample, double medium filtration mesh screen 6 can be installed in concentration tube 5, and concentration tube 5 is connect with suction pump,
Or air vent 11 is closed, promote liquid to flow out with the mode of manual pressure.
3, sample collection:
Tongs 4 are opened, 15-20ml after the sample of decanting zone 12 and enrichment region 13 is mixed opens lid 1, by sample
It imports in 50ml sample bottle.10ml liquid is added from hard injection pipe 2 again, concussion cleaning carried out to sample sack, and from concentration tube 5
Mesh screen 6 is filtered in interior taking-up, is rinsed again with a small amount of sample stoste filtering mesh screen 6, the liquid after flushing is collected into 50ml sample
Constant volume is carried out in product bottle.
Claims (8)
1. including bag body (10) and the closing device in bag body (10) oral area is arranged in a kind of field planktonic organism quantitative collection bag,
It is characterized by: the oral area of the bag body (10) is necking, tongs (4) are installed at necking, pacify on bag body (10) top
Equipped with the concentration tube (5) being connected to bag body (10) inner cavity, filtering mesh screen is installed in the junction of concentration tube (5) and bag body (10)
(6), air vent (11) are installed in bag body (10) lower part.
2. field planktonic organism quantitative collection bag according to claim 1, it is characterised in that: the closing device include with
The hard injection pipe (2) of bag body (10) oral area connection, is connected with lid (1) in the mouth thread of hard injection pipe (2).
3. field planktonic organism quantitative collection bag according to claim 1, it is characterised in that: the periphery of the bag body (10)
It is provided with the edge sealing (3) that a circle is connect with bag body (10), strip-shaped hole (8) are installed on the top of edge sealing (3), in edge sealing (3)
Lower central offer circular hole (9).
4. field planktonic organism quantitative collection bag according to claim 1, it is characterised in that: the outer wall of the bag body (10)
On be provided with graduation mark (7).
5. field planktonic organism quantitative collection bag according to claim 1, it is characterised in that: the mouth of the concentration tube (5)
Portion is provided with plug.
6. a kind of application method based on field planktonic organism quantitative collection bag described in Claims 1 to 5 any one, special
Sign be the following steps are included:
Step 1: sample acquires: concentration tube (5) and air vent (11), opening lid (1) and tongs (4) are closed, from quantitatively adopting
Sample is injected into bag body (10) from hard injection pipe (2) in sample device, lid (1) is tightened after the completion of injection, sampling will be finished writing
The label paper of information is attached on bag body (10), i.e. completion sample collecting work；
Step 2: sample concentration: being hung at the circular hole (9) of bag body (10) with hook or cord, be inverted bag body (10) 2 hours
More than, after suspended matter precipitating, tongs (4) can be clipped in bag body (10) necking position, decanting zone (12) are isolated
Prevent Secondary Disturbance；Concentration tube (5) and air vent (11) are opened, allows water to flow out from concentration tube (5), by residual sample in sample upper layer
Product concentration, until residue 5-10ml；
Step 3: sample collection: opening tongs (4), after the sample of decanting zone (12) and enrichment region (13) is mixed, open lid
Sub (1) by Sample introduction sample bottle, then is added liquid from hard injection pipe (2) and carries out concussion cleaning to sample sack, and from dense
Filtering mesh screen (6) is taken out in the draw (5), is rinsed again with a small amount of sample stoste filtering mesh screen (6), and the liquid after flushing is received
Collect and carries out constant volume in sample bottle.
7. the application method of field planktonic organism quantitative collection bag according to claim 6, it is characterised in that: carry in field
When bag body (10), finger is protruded into strip-shaped hole (8) and is guided and supported entire bag body (10), or protruded into strip-shaped hole (8) with Fastener for climbing mountain,
With bag body (10) is suspended on.
8. the application method of field planktonic organism quantitative collection bag according to claim 6, it is characterised in that: step 2
In, such as enriched sample, double medium filtration mesh screen (6) can be installed in concentration tube (5), and by concentration tube (5) and suction pump
Connection, liquid is extracted out, or closes air vent (11), promotes liquid to flow out with the mode of manual pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659479.2A CN110220746B (en) | 2019-07-22 | 2019-07-22 | Quantitative collection bag for field plankton and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659479.2A CN110220746B (en) | 2019-07-22 | 2019-07-22 | Quantitative collection bag for field plankton and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110220746A true CN110220746A (en) | 2019-09-10 |
| CN110220746B CN110220746B (en) | 2024-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910659479.2A Active CN110220746B (en) | 2019-07-22 | 2019-07-22 | Quantitative collection bag for field plankton and use method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167190A (en) * | 2020-09-29 | 2021-01-05 | 北部湾大学 | Water layer-changing plankton collection device |
| CN113069808A (en) * | 2021-05-10 | 2021-07-06 | 安徽理工大学环境友好材料与职业健康研究院(芜湖) | Concentrated filter equipment of plankton sample collection |
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| CN208621407U (en) * | 2018-08-31 | 2019-03-19 | 江西省水利科学研究院 | A kind of portable phytoplankton quantifies sample concentration device |
| CN210198784U (en) * | 2019-07-22 | 2020-03-27 | 茅台学院 | Open-air plankton ration collection bag |
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- 2019-07-22 CN CN201910659479.2A patent/CN110220746B/en active Active
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| AU2011244840A1 (en) * | 2003-11-19 | 2011-11-24 | Noble House Group Pty. Ltd. | Improved sterile sampling methods and apparatus |
| JP2009240274A (en) * | 2008-03-31 | 2009-10-22 | Chugoku Electric Power Co Inc:The | Plankton concentration device and plankton concentration system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167190A (en) * | 2020-09-29 | 2021-01-05 | 北部湾大学 | Water layer-changing plankton collection device |
| CN112167190B (en) * | 2020-09-29 | 2021-09-28 | 北部湾大学 | Water layer-changing plankton collection device |
| CN113069808A (en) * | 2021-05-10 | 2021-07-06 | 安徽理工大学环境友好材料与职业健康研究院(芜湖) | Concentrated filter equipment of plankton sample collection |
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| CN110220746B (en) | 2024-03-01 |
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