CN110220746B - A kind of field plankton quantitative collection bag and its use method - Google Patents
A kind of field plankton quantitative collection bag and its use method Download PDFInfo
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- CN110220746B CN110220746B CN201910659479.2A CN201910659479A CN110220746B CN 110220746 B CN110220746 B CN 110220746B CN 201910659479 A CN201910659479 A CN 201910659479A CN 110220746 B CN110220746 B CN 110220746B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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Abstract
本发明公开了一种野外浮游生物定量采集袋及使用方法，该采集袋包括袋体和设置在袋体口部的封口装置，所述袋体的口部为缩口，在缩口处安装有止水夹，在袋体上部安装有与袋体内腔连通的浓缩管，在浓缩管与袋体的连接处安装有过滤网筛，在袋体下部安装有放气孔。与传统的定量采样、样品运输、沉淀、浓缩及分离装置相比，本发明携带方便的同时，大大提高采样车的空间利用率，克服了样品二次转移、沉淀的浮游植物容易被扰动的缺点，以及分离操作时间长的问题。同时，由于袋体采用耐酸材料制作，袋体的耐用性高，更加高效、耐用，并且能在野外进行操作，能显著提高工作效率。
The invention discloses a field plankton quantitative collection bag and a method of use. The collection bag includes a bag body and a sealing device arranged at the mouth of the bag body. The mouth of the bag body is a shrinking mouth, and a sealing device is installed at the shrinking mouth. The water stop clamp is equipped with a concentration tube connected to the cavity of the bag body on the upper part of the bag body, a filter screen is installed at the connection between the concentration tube and the bag body, and a vent hole is installed on the lower part of the bag body. Compared with traditional quantitative sampling, sample transportation, sedimentation, concentration and separation devices, the present invention is easy to carry, greatly improves the space utilization of the sampling vehicle, and overcomes the shortcomings of secondary transfer of samples and the easy disturbance of precipitated phytoplankton. , as well as the problem of long separation operation time. At the same time, because the bag body is made of acid-resistant materials, it has high durability, is more efficient and durable, and can be operated in the field, which can significantly improve work efficiency.
Description
Technical Field
The invention relates to a quantitative collection bag for field plankton and a use method thereof, belonging to the technical field of water ecological environment monitoring.
Background
The eutrophication of water body causes the increase of algae biomass and the change of community structure composition, thereby causing instability of an ecological system and causing immeasurable loss for national economic development. In recent years, the water environment and water resource management departments bring phytoplankton monitoring into the conventional monitoring range, and quantitative analysis of phytoplankton is a basic work in water ecology research and is also an indispensable part in phytoplankton monitoring.
Quantitative analysis of phytoplankton according to the technical regulations for monitoring phytoplankton in inland water areas, SL733-2016, states: the sampling amount of the phytoplankton quantitative sample is 1000-2000 mL, the water body with lower phytoplankton biomass is increased to 3000-5000 mL as appropriate, and the quantitative sample is required to be provided with parallel samples according to 10-20% of the total number of the collected samples; pouring a quantitative water sample collected and fixed in the field into a precipitator in a laboratory, standing for 48 hours, pumping out supernatant liquid by using a siphon pipe with 25-mesh bolting silk, and controlling the siphon flow rate (if disturbance occurs, standing again for precipitation); transferring the sediment of which 20-25 mL is remained into a quantitative bottle after siphoning, flushing the container with a little supernatant liquid for several times, and finally fixing the volume to 30mL. Because more samples are often needed, the traditional sampling bottle cannot effectively utilize space due to morphological problems, and occupies more space; meanwhile, when the traditional device is used for concentrating and separating phytoplankton, the collected sample needs to be poured into a precipitator, so that the sample has certain loss; in addition, the siphoning is time-consuming and labor-consuming after 48 hours of precipitation, and the siphoning is easy to cause disturbance precipitation, and the reprecipitation is needed for a plurality of hours. This method is therefore time-consuming and labor-consuming, especially when a large amount of sample needs to be concentrated in a short period of time, and is not efficient.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the field plankton quantitative collection bag which has the advantages of simple structure, convenient operation, time and labor saving and high experimental efficiency and the use method thereof, so as to overcome the defects in the prior art.
The technical scheme of the invention is realized as follows: the utility model provides a bag is gathered to field plankton ration, includes the bag body and sets up the closing device at the bag body oral area, the oral area of the bag body is the throat, installs the stagnant water in the throat department and presss from both sides, installs the concentrate pipe with the internal chamber intercommunication of bag in bag body upper portion, installs the filtration mesh screen in the junction of concentrate pipe and the bag body, installs the bleed hole in bag body lower part.
Further, the sealing device comprises a hard injection pipe connected with the mouth part of the bag body, and a cover is connected with the mouth part of the hard injection pipe in a threaded manner.
Further, a circle of sealing edge connected with the bag body is arranged on the periphery of the bag body, a strip-shaped hole is formed in the upper portion of the sealing edge, and a round hole is formed in the center of the lower portion of the sealing edge.
Further, scale marks are arranged on the outer wall of the bag body.
Further, a plug is arranged at the opening part of the concentration tube.
Meanwhile, the invention provides a using method based on the field plankton quantitative collection bag, which comprises the following steps:
step one, sample collection: closing the concentration tube and the air vent, opening the cover and the water stop clamp, injecting the sample into the bag body from the hard injection tube from the quantitative sampler, screwing the cover after the injection is finished, and sticking the label paper with the sampling information on the bag body to finish the sample collection work;
step two, sample concentration: hanging the bag body from the round hole of the bag body by using a hook or a string, inverting the bag body for more than 2 hours, and after suspended matters are precipitated, clamping a water stop clamp at the position of the shrinkage opening of the bag body, and isolating a sedimentation area to prevent secondary disturbance; opening the concentration tube and the air vent, allowing water to flow out of the concentration tube, and concentrating the residual sample in the upper layer of the sample until 5-10ml remains;
step three, sample collection: opening the water stop clamp, mixing the samples in the sedimentation area and the concentration area, opening the cover, introducing the samples into the sample bottle, adding liquid from the hard injection pipe to shake and clean the sample bag, taking out the filter mesh screen from the concentration pipe, flushing again by using a small amount of sample stock solution filter mesh screen, and collecting the flushed liquid into the sample bottle for constant volume.
In the method, when the bag body is carried in the field, fingers extend into the strip-shaped holes to carry the whole bag body, or the mountain climbing buckles extend into the strip-shaped holes to hang the bag body on the body.
In the second step, if a high concentration sample is encountered, a double-layer filtering mesh screen can be arranged in the concentration tube, the concentration tube is connected with a water pump, the liquid is pumped out, or the air vent is closed, and the liquid is promoted to flow out in a manual pressurization mode.
By adopting the technical scheme, the invention has the advantages that: compared with the traditional quantitative sampling, sample transportation, precipitation, concentration and separation device, the device is convenient to carry, greatly improves the space utilization rate of the sampling vehicle, and overcomes the defects that the phytoplankton subjected to secondary transfer and precipitation of the sample is easy to be disturbed and the problem of long separation operation time. Meanwhile, the bag body is made of acid-resistant materials, so that the bag body is high in durability, more efficient and durable, can be operated in the field, and can remarkably improve the working efficiency.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
fig. 2 is a schematic diagram of the structure of the present invention in its implementation.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiments of the invention: referring to fig. 1, a quantitative collection bag for field plankton comprises a bag body 10 and a sealing device arranged at the opening of the bag body 10, wherein the opening of the bag body 10 is a necking, a water stop clamp 4 is arranged at the necking, a concentration tube 5 communicated with the inner cavity of the bag body 10 is arranged at the upper part of the bag body 10, a filtering mesh screen 6 is arranged at the joint of the concentration tube 5 and the bag body 10, the aperture of the filtering mesh screen 6 is smaller than 10um, and a vent hole 11 is arranged at the lower part of the bag body 10. The sealing device comprises a hard injection pipe 2 connected with the mouth part of a bag body 10, and a cover 1 is connected with the mouth part of the hard injection pipe 2 in a threaded manner. The periphery of the bag body 10 is provided with a circle of sealing edge 3 connected with the bag body 10, a strip-shaped hole 8 is formed in the upper part of the sealing edge 3, and a round hole 9 is formed in the center of the lower part of the sealing edge 3; scale marks 7 are arranged on the outer wall of the bag body 10; the mouth of the concentration tube 5 is provided with a plug.
Referring to fig. 2, the working principle of the present invention is as follows:
1. sample collection:
closing the concentration tube 5 and the air vent 11, opening the cover 1 and the water stop clamp 4, injecting a sample from the hard injection tube 2 into the bag body 10 from the quantitative sampler, screwing the cover 1 after injection is finished, and attaching label paper with sampling information on the bag body 10 to finish the sample collection work; when the bag body 10 is carried in the field, fingers can be inserted into the strip-shaped holes 8 to carry the whole bag body 10, or the mountain climbing buckles can be inserted into the strip-shaped holes 8 to hang the bag body 10 on a human body.
2. Sample concentration:
a. hanging the bag body 10 from the round hole 9 of the bag body 10 by using a hook or a string, inverting the bag body 10 for more than 2 hours, and clamping the water stop clamp 4 at the necking position of the bag body 10 after the main suspended matters are precipitated, so as to isolate the main precipitation area to prevent secondary disturbance; b. opening the concentration tube 5 and the air vent 11 to allow water to flow out of the concentration tube 5, and concentrating the residual sample in the upper layer of the sample until 5-10ml remains; if a high-concentration sample is met, a double-layer filter mesh screen 6 can be arranged in the concentration tube 5, the concentration tube 5 is connected with a water pump, or the air release hole 11 is closed, so that liquid outflow is promoted by manual pressurization.
3. Sample collection:
the water stop clamp 4 was opened, 15-20ml of the mixture of the samples in the sedimentation zone 12 and the concentration zone 13 was then mixed, and the lid 1 was opened to introduce the sample into a 50ml sample bottle. Then 10ml of liquid is added from the hard injection tube 2 to shake and clean the sample bag, the filter mesh screen 6 is taken out from the concentration tube 5, a small amount of sample stock solution is used for washing the filter mesh screen 6 again, and the washed liquid is collected into a 50ml sample bottle for constant volume.
Claims (3)
1. The utility model provides a method of using of field plankton ration collection bag, field plankton ration collection bag includes the bag body (10) and sets up the closing device at the mouth of the bag body (10), its characterized in that: the mouth of the bag body (10) is a necking, a water stop clamp (4) is arranged at the necking, a concentration pipe (5) communicated with the inner cavity of the bag body (10) is arranged at the upper part of the bag body (10), a filter mesh screen (6) is arranged at the joint of the concentration pipe (5) and the bag body (10), and a deflation hole (11) is arranged at the lower part of the bag body (10); the sealing device comprises a hard injection pipe (2) connected with the mouth part of the bag body (10), and a cover (1) is connected with the mouth part of the hard injection pipe (2) in a threaded manner; the periphery of the bag body (10) is provided with a circle of sealing edge (3) connected with the bag body (10), a strip-shaped hole (8) is formed in the upper part of the sealing edge (3), and a round hole (9) is formed in the center of the lower part of the sealing edge (3); scale marks (7) are arranged on the outer wall of the bag body (10); a plug is arranged at the mouth part of the concentration tube (5);
the using method of the field plankton quantitative collection bag comprises the following steps:
step one, sample collection: closing the concentration tube (5) and the air vent (11), opening the cover (1) and the water stop clamp (4), injecting a sample into the bag body (10) from the hard injection tube (2) from the quantitative sampler, screwing the cover (1) after the injection is finished, and attaching label paper with the written sampling information on the bag body (10), thereby completing the sample collection work;
step two, sample concentration: hanging the bag body (10) from a round hole (9) of the bag body (10) by using a hook or a string, inverting the bag body (10) for more than 2 hours, and after suspended matters are precipitated, clamping the water stop clamp (4) at the necking position of the bag body (10), and isolating the sedimentation area (12) to prevent secondary disturbance; opening the concentration tube (5) and the air vent (11), allowing water to flow out of the concentration tube (5), and concentrating the residual sample in the upper layer of the sample until 5-10ml remains;
step three, sample collection: opening a water stop clamp (4), mixing samples in a sedimentation area (12) and a concentration area (13), opening a cover (1), introducing the samples into a sample bottle, adding liquid from a hard injection pipe (2), carrying out vibration cleaning on a sample bag, taking out a filter mesh screen (6) from a concentration pipe (5), washing again by using a small amount of sample stock solution filter mesh screen (6), and collecting the washed liquid into the sample bottle for constant volume.
2. A method of using a field plankton quantification collection bag according to claim 1, wherein: when the bag body (10) is carried in the field, fingers extend into the strip-shaped holes (8) to carry the whole bag body (10), or climbing buckles extend into the strip-shaped holes (8) to hang the bag body (10) on a human body.
3. A method of using a field plankton quantification collection bag according to claim 1, wherein: in the second step, if a high-concentration sample is met, a double-layer filter mesh screen (6) can be arranged in the concentration tube (5), the concentration tube (5) is connected with a water pump, liquid is pumped out, or the air release hole (11) is closed, and the liquid is promoted to flow out in a manual pressurization mode.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910659479.2A CN110220746B (en) | 2019-07-22 | 2019-07-22 | A kind of field plankton quantitative collection bag and its use method |
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| CN201910659479.2A CN110220746B (en) | 2019-07-22 | 2019-07-22 | A kind of field plankton quantitative collection bag and its use method |
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| CN110220746B true CN110220746B (en) | 2024-03-01 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110711609A (en) * | 2019-10-12 | 2020-01-21 | 程斯婷 | Quantitative sample retention device and method for liquid in container |
| CN112167190B (en) * | 2020-09-29 | 2021-09-28 | 北部湾大学 | Water layer-changing plankton collection device |
| CN113069808A (en) * | 2021-05-10 | 2021-07-06 | 安徽理工大学环境友好材料与职业健康研究院(芜湖) | Concentrated filter equipment of plankton sample collection |
| CN115436097B (en) * | 2022-09-19 | 2025-09-05 | 中国环境科学研究院 | A device capable of quantitatively collecting surface phytoplankton samples |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US10221388B2 (en) * | 2014-02-06 | 2019-03-05 | Nancy Leland | Method and device for plankton separation |
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| AU2011244840A1 (en) * | 2003-11-19 | 2011-11-24 | Noble House Group Pty. Ltd. | Improved sterile sampling methods and apparatus |
| JP2009240274A (en) * | 2008-03-31 | 2009-10-22 | Chugoku Electric Power Co Inc:The | Plankton concentration device and plankton concentration system |
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