CN109548559A - A kind of only carpogenic ganoderma lucidum cultivation method - Google Patents
A kind of only carpogenic ganoderma lucidum cultivation method Download PDFInfo
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- CN109548559A CN109548559A CN201811573573.8A CN201811573573A CN109548559A CN 109548559 A CN109548559 A CN 109548559A CN 201811573573 A CN201811573573 A CN 201811573573A CN 109548559 A CN109548559 A CN 109548559A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
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Abstract
The invention discloses a kind of only carpogenic ganoderma lucidum cultivation methods, and this method comprises the following steps: the selection of cultivation area；The selection of cultivation of glossy ganoderma matrix；High-quality lucidum strain is selected；The production of ganoderma lucidum bacteria stick；Ganoderma lucidum does not take off bag soil covering culture；Water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process；Ganoderma lucidum harvesting.Only carpogenic ganoderma lucidum cultivation method through the invention can preferably alleviate the negative effect for even being eliminated continuous cropping obstacle to cultivation of glossy ganoderma, obtain ganoderma lucidum raw material secure in safety and quality.
Description
Technical field
The present invention relates to edible and medical fungi cultivation fields, more particularly to a kind of only carpogenic ganoderma lucidum cultivation method.
Background technique
In China, ganoderma lucidum is the medicinal fungi of great legendry, is classified as by Shennong's Herbal and Compendium of Material Medica etc.
Product are the precious Chinese medicine of nourishing and fit keeping function, righting reinforcing, have long term usage make light of one's life by commiting suicide macrobiosis the effect of.Modern medicine study is proved ganoderma lucidum
With a variety of biological effects such as antitumor, adjusting is immune, liver protection, tranquilizing and allaying excitement, blood pressure lowering.Currently, its by " Chinese Pharmacopoeia ",
" U.S.'s herbal medicine pharmacopeia and treatment summary " (American Herbal Pharmacopoeia and Therapeutic
) etc. Compendium include, this shows that the medical value of ganoderma lucidum has obtained extremely wide approval.With integration of drinking and medicinal herbs, health
The idea of health is increasingly big well-established, main Chinese medicine one of of the ganoderma lucidum as " ill cure the disease, disease-free health ", by
More and more consumers is eaten.The annual value of production of present China ganoderma lucidum industry is up to 15,000,000,000 yuan；Korea Spro of the population less than 50,000,000
The annual consumption of state ganoderma lucidum also reaches 4500 tons.
Increasingly vigorous with the market demand, the cultivation scale of ganoderma lucidum also expands therewith.Wherein, the overwhelming majority is covered for bacteria stick
Soil cultivation.Soil covering culture has de- bag and half de- two kinds of forms of bag.Therefore, bacteria stick all can sufficiently connect in soil covering culture with soil
Touching.Carry the pollutants such as some remainss of pesticide, heavy metal and some other harmful microorganisms etc. in soil.Studies have shown that
Edible and medicinal fungi all has the characteristic that heavy metal is shifted, adsorbed from soil and cultivation matrix.Therefore when the nuisance in soil
Matter is more than the self-purification capacity of soil, will cause soil physico-chemical characteristic and variation functionally, the i.e. meeting of microbial activities therein
Limited, harmful substance or downstream product gradually accumulate in the soil and pass through " soil → plant (or edible fungus) → people
Body ", or be absorbed by the body indirectly by " soil → water → human body ", to jeopardize health.The problem of another can not be ignored
It is exactly cultivation and its adjoint pest problem of growth and development process of ganoderma lucidum.It is some studies have shown that there are mite class, termite in soil
The pests such as class are fond of ganoderma lucidum bacteria stick (mycelium), make a big impact to cultivation of glossy ganoderma.Once this kind of insect pest is serious, farmer is just
Strong toxicity, the residual life longer pesticide that some such as dicofols etc can be applied, bring greater risk to food safety.
Therefore, current cultivation of glossy ganoderma mode makes ganoderma lucidum raw material and products thereof be faced with the exceeded risk of heavy metal and pesticide remnant.
Research shows that there are continuous cropping obstacle phenomenons on cultivation of glossy ganoderma, i.e. the continuous cultivating ganoderma on same plot, ganoderma lucidum
Yield and quality, which will appear, to be remarkably decreased.Thus, farmer will almost strive to find new plot every year and carry out cultivating ganoderma.This
It has resulted in soil and the cultivating facilities utilization efficiency such as the greenhouse established thereon is extremely low, correspondingly increased production cost.
Currently, there are also the exploration and trial that are carried out for the problem in cultivation of glossy ganoderma, for example, some mechanisms or
Individual is solidified the place of cultivating ganoderma using cement, and bacteria stick and following soil is isolated, and is shipped new soil from other places and is carried out
Cultivation of glossy ganoderma, it is desirable to mitigate the continuous cropping obstacle effect of ganoderma lucidum by frequently more soil removal and replacement.This method also faces two aspects
Problem: first is that frequently more soil removal and replacement needs extensive source, while these soil are if appropriate for cultivation of glossy ganoderma；Second is that being essentially to these
Agricultural land is solidified, and can be changed the physicochemical property of soil completely, be caused this block soil that can not carry out other crops in the future
Production.Cultivation of glossy ganoderma is realized using batch production there are also some enterprise expectations, but from the point of view of practice result, effect is very at present
It is bad.Here there are two the restraining factors of aspect: first is that production cost is excessively high；The other is in production, there is also some technologies
Problem.
Based on above-mentioned, in order to obtain the ganoderma lucidum fruitbody of edible safety, while continuous cropping obstacle pair even is eliminated in order to mitigate
Negative effect caused by ganoderma lucidum industry reduces production cost, needs to develop a kind of new ganoderma lucidum cultivation method.
Summary of the invention
The purpose of the present invention is to provide a kind of only carpogenic ganoderma lucidum cultivation methods comprising following steps:
The selection of cultivation area；The selection of cultivation of glossy ganoderma matrix；High-quality lucidum strain is selected；The production of ganoderma lucidum bacteria stick；Spirit
Sesame does not take off bag soil covering culture；Water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process；Ganoderma lucidum harvesting；
Wherein cultivation of glossy ganoderma matrix is juggle and sawdust materials；
Wherein juggle is big juggle (25-35cm)；Compost selects sawdust materials (to make water content using Shi Jiashui
70%)；
Specifically, a kind of only carpogenic ganoderma lucidum cultivation method of the invention, includes the following steps:
(1) the selection of cultivation area: cultivation area is far from highway, linear distance 500m or more；Because motor vehicle emission
Contain heavy metal component in tail gas；
(2) the selection of cultivation matrix: the hardwood of no fragranced is used equally for cultivation of glossy ganoderma, is preferred with Cyclobalanopsiss timber, wood
Segment length is 25-35cm；Compost selects sawdust materials；The composition of preferred sawdust materials are as follows: sawdust 78%, wheat bran
13%, corn flour 7%, sucrose 1%, land plaster or calcium carbonate 1% make water content 70% using Shi Jiashui；
The pesticide and content of beary metal of cultivation matrix will meet national standard and professional standard；
(3) high-quality lucidum strain is selected: ganoderma strain being inoculated on PDA plate or similar solid medium, is protected from light training
It supports 4-6 days, the bacterium colony of formation is that rounding, mycelium are sturdy and fine and close, as more excellent strain；
Cultivar is advisable with being not difficult to pulverize；Culture spawn incubation time is too short, will affect the development of ganoderma lucidum bacteria stick, incubation time
It is too long, cause cultivation strain to be difficult to pulverize, influences rapid field planting of the cultivation on sawdust kind in bacteria stick and effective use cultivation matrix；
(4) the production of ganoderma lucidum bacteria stick:
I, pack and sterilizing
The juggle cut is packed, fills sawdust materials at its both ends, sack can not prick to obtain tension；Ensure hot steam energy
Enough normal disengaging, juggle can be sterilized thoroughly；
The stacking of juggle, which is subject to, can guarantee that hot steam is unimpeded, the temperature in sterilization facility to be promoted to rapidly 98 DEG C with
On.
II, inoculation and culture (incubation time of bacteria stick is 2.5-4 months)
Cultivar is seeded on the sawdust materials at juggle both ends；Culture is stacked in a manner of wall；In the training period, it gives
Ventilation appropriate is given, and carries out the adjustment of bacteria stick or more hierarchical position, it is ensured that growth and development dynamics are more consistent；
(5) ganoderma lucidum does not take off bag soil covering culture:
I, greenhouse is built
In the bacteria stick earthing previous moon, fine day is selected to turn over the soil for the cultivation area selected in step (1), clearly
It cuts weeds, stone etc., after solar exposure a few days and sandy soil whiten, builds furrow；High 20cm of furrow or so, the wide 1.5-1.8 m of furrow, furrow are long
It is general to require to be no more than 20m depending on landform, in order to avoid influence ventilation；It should also the row of holding successfully in advance in the place that mountain torrents may occur
Great Gulch；Every two furrow build the vinyl house of 1.8-2.2m high, are covered with the plastic film of 7m wide above, to adjust the epidemic disaster in canopy,
It covers 2 layers of black sunshade net again on film, to obtain the suitable homogenous diffusion light of light intensity, meets ganoderma lucidum growth and development to light
Needs；A plastics Small plastic shed can also be built on every furrow, then builds the greenhouse an of flat-top, high 2.5m or so, cover 2 layers
Sunshade net；
The arrangement of II, bacteria stick and earthing
After ganoderma lucidum bacteria stick is trained ripe, it is transported to the arranged cultivation area put in order, not slough surface
Sack, directly by its with horizontal or vertical mode be arranged in ground furrow on, fine earth (granularity 0.5-1.0cm) is covered in
On bacteria stick.If bacteria stick is horizontal arrangement, to guarantee that the upper space of bacteria stick is just exposed；It is if vertical arrangement, then true
Just expose the one end for protecting bacteria stick；
(6) water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process:
After bacteria stick earthing, several irrigation canals and ditches, 30-35cm every wide, deep 40-60cm should be held successfully in canopy；It maintains to have in irrigation canals and ditches
Water can ensure that the interior humidity of cultivation greenhouse is high compared with outside canopy, facilitate the growth and development of ganoderma lucidum；
Bacteria stick earthing is after 10-15 days, and 1. bacteria stick is vertical, cuts along sack tether, but not strut sack；Or 2.
Bacteria stick be it is horizontal, then upper 1-2 small opennings are opened on the face that bacteria stick exposes earth's surface；
It will form former base through opening after a period of time, the water for injecting how many volume judged according to the color of former base；If
Former base or former base upper surface be it is light brown, then fill the water 30-40ml in the sawdust medium matter of both ends；If former base upper surface has
Yellow will then fill the water 50-70ml in the sawdust medium matter of both ends；If entire former base is flat-white, and is touched and felt with hand
Feel very hard, illustrates that water shortage is serious, then 80-150ml should be filled the water immediately in the sawdust medium matter of both ends, to supplement growth and development
The moisture consumed in the process；If above-mentioned processing fails that former base color is made to change, should continue to fill the water, it is ensured that ganoderma lucidum normally gives birth to
It is long to need；
When former base is gradually differentiated to form cap, and after carrying out horizontal cross growth, to be determined according to cap edge color
Whether to spray water；If cap edge, secondary side edge are respectively cream-coloured, ecru, water spray is not had to temporarily；If cap edge is
(shallow) yellow, then should spray water；If the color at cap edge is buff, increase the water spray frequency；
When ganoderma lucidum cap size is close to the market demand, the water spray frequency should be gradually decreased until stopping water spray；
(7) ganoderma lucidum fruitbody harvests:
After ganoderma lucidum maturation and edge thickness is more than that 1cm can be harvested；
After ganoderma lucidum harvests, it should dry or dry in time, make its water content lower than 13%；And carry out storage.
Since the juggle wherein for cultivation is big juggle, cultivating bag specification is big, the nutritional ingredient in matrix compared with horn of plenty,
It can not be consumed in a production cycle, so after the harvesting of First Year ganoderma lucidum maturation, remaining nutritional ingredient in matrix
Also it is enough to maintain the normal growth and development of second year ganoderma lucidum.
Second year the middle ten days and the last ten days in March after step (7) after ganoderma lucidum harvesting, temperature is stablized at 10-15 DEG C or more, in bacterium
It injects water in the sawdust of stick both ends, mycelia is promoted in bacteria stick to sprout growth；After former base is formed, according to the color of former base, note is determined
The amount of water；When former base is differentiated to form cap, and after carrying out horizontal cross growth, to be determined whether to according to cap edge color
Water spray；After ganoderma lucidum maturation and edge thickness is more than that 1cm can be harvested.(second year is (7) (6) carried out with step according to step
Cultivation management.)
The step of next third year and the 4th year can be according still further to front First Years, second year repeats cultivation production.
A kind of only carpogenic ganoderma lucidum cultivation method of the invention, have it is following the utility model has the advantages that
A kind of only carpogenic ganoderma lucidum cultivation method of the invention, by big segment wood cultivated, First Year bacteria stick, which is gone to the field, to be covered
After soil cultivation, First Year is harvested 1 time, and second year can harvest 1 time again.Bacteria stick is remake third year again to go to the field soil covering culture,
It harvests in third year 1 time, then harvests 1 time within the 4th year.The total output of the first two years and rear 2 years total outputs do not have marked difference.It can
To carry out the cultivation and harvest of the 5th year and the 6th year, the 7th year and the 8th year ... on same plot, and do not have to cultivation area
There is destruction.
Therefore, only carpogenic ganoderma lucidum cultivation method through the invention, can preferably alleviate and even be eliminated continuous cropping
Negative effect of the obstacle to cultivation of glossy ganoderma obtains ganoderma lucidum raw material secure in safety and quality.It is not significantly changing simultaneously
Under the premise of soil physico-chemical property, the utilization rate of cultivating facility for greatly improving soil and establishing thereon can significantly reduce production
Cost increases the income of sesame agriculture.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiment 1 (between 2013.11-2017.11 olympiad, carries out continuous 4 years cultivation of glossy ganoderma in same plot)
Lucidum strain: " Shanghai agriculture ganoderma lucidum No. 4 " by Academy of Agricultural Sciences, Shanghai City breeding, which hardly produces Reishi sporule
Powder, high yield ganoderma lucidum fruitbody.
1. the selection of cultivation area
The plot that Shaanxi Province, mulberry field township, Hanzhong City Xixiang County is passed through without expressway within the border is had chosen, topography is open herein,
Irrigation conditions is good.
2. the selection of cultivation matrix
Cultivation of glossy ganoderma matrix is juggle (Qinggang oak) and sawdust materials；
Wherein juggle is truncated into big juggle (30cm)；The composition of sawdust materials are as follows: 78% sawdust, 13% wheat bran, 7% corn
Powder, 1% sucrose, 1% land plaster, when use, add water mixing to make water content 70%；
Pesticide residue and heavy metal analysis are carried out to water used in sawdust materials, juggle and cultivation:
First by 1-2 hours of boiling waterbaths such as sawdust, wheat bran and corn flour in juggle and sawdust materials, by adding
Thermal evaporation moisture, it is rear to be freeze-dried, send qualified testing agency (SGS group) detect common type pesticide residue and lead,
The content of beary metal such as arsenic, mercury, cadmium；Also carry out that agriculture is residual and heavy metal analysis to water.
Testing result meets country or professional standard, can come into operation.
3. the selection of high-quality lucidum strain
It will be seeded to PDA plate center after the activation of " Shanghai agriculture ganoderma lucidum No. 4 " bacterial strain, after culture 5 days, observes colonial morphology, choosing
It is sturdy and be close to the bacterial strain of plate as parent species to select bacterium colony rounding, mycelium, carries out subsequent original seed and cultivar makes.In original
During kind and cultivar culture, growing way situation is often observed.The original seed or cultivar that are infected with miscellaneous bacteria will be rejected in time.It plants
It cultivates and has cultivated 30-35 days, cultivar at this time is easier to pulverize for being inoculated with.
4. the production of ganoderma lucidum bacteria stick
(1) pack and sterilizing
Timber is cut into section according to the length of 30cm, is fitted into the polyethylene plastic bag of 36cm*66cm specification, in its juggle
Both ends filling thickness be about 1.0-1.2cm sawdust materials (water content of the compost is 70% or so, and humidity is equal
It is even) covering juggle section.To ensure that hot steam can be passed in and out normally, juggle can be the tightness that sack is pricked by thorough sterilization
It is quasi-.
The stacking of juggle does not provide, is subject to and can guarantee that hot steam is unimpeded, and the temperature in sterilization facility will be promoted rapidly
To 98 DEG C, and continue heat preservation 30 hours.
(2) inoculation and culture (incubation time is 3-4 months)
After sterilizing, temperature is down to 40 DEG C or so in facility subject to sterilization, and juggle is carried to sterile-processed mistake
In transfer room (canopy or case).Emgloves is put on, with 75% alcohol surface sterilization.Cultivar is firmly pulverized, juggle is seeded to
On the sawdust materials at both ends, sack is tied, culture is stacked in a manner of wall.In the training period, certain ventilation is given, to protect
Demand of the bacteria stick growth to oxygen is demonstrate,proved, while adjusting bacteria stick hierarchical position up and down, it is ensured that the speed of growth is more consistent.In culture
Middle and later periods, culturing room's (canopy) is interior to place the basin for filling with water, to guarantee requirement of the growth to humidity of bacteria stick.
5. ganoderma lucidum does not take off bag soil covering culture
(1) greenhouse is built
In the bacteria stick earthing previous moon, fine day is selected to turn over the soil of the cultivation area selected in step 1, removed
Weeds, stone etc. build furrow after solar exposure a few days and sandy soil whiten.High 20cm of furrow or so, the wide 1.5-1.8m of furrow, furrow it is long according to
It is general to require to be no more than 20m depending on landform, in order to avoid influence ventilation.A plastics Small plastic shed is built on every furrow, then utilizes bamboo
Etc. the greenhouse for building a flat-top, high 2.5m or so, 2 layers of sunshade net are spread, it is full to obtain the suitable homogenous diffusion light of light intensity
Needs of the sufficient ganoderma lucidum growth and development to light；
(2) bacteria stick arrangement and earthing
After ganoderma lucidum bacteria stick is reached maturity, and it is local the temperature rises and when maintaining 12-15 DEG C, as far as possible by its intact nothing
In the transport to cultivation area greenhouse of damage ground, the sack of bacterium rod surface is not sloughed；If any breakage, breakage is closed with adhesive tape.It presses
It is arranged in vertically on ground furrow according to the specification that line-spacing, spacing are 5-6cm, and fine earth (granularity is 0.5-1.0 cm) is covered
It is placed on bacteria stick, exposes the upper end of bacteria stick just.
6. water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process:
After bacteria stick earthing, 3 irrigation canals and ditches, 28cm every wide, deep 45cm are held successfully in canopy.There is more clear water in irrigation canals and ditches, often
It is replaced every a period of time primary, it is ensured that humidity when weather is slightly dry in canopy in local space is high compared with outside canopy, beneficial to the life of ganoderma lucidum
Long development.
Bacteria stick earthing opened upper 1-2 small opennings after 10-15 days on the face that bacteria stick exposes earth's surface；It is opened after a period of time
Will form former base at mouthful, when former base or former base upper surface be it is light brown, then fill the water 40ml in the sawdust medium matter of both ends；If former
There are some yellow in base upper surface, then to fill the water 60ml in the sawdust medium matter of both ends；If entire former base is flat-white, and
Feel very hard with hand touching, then should fill the water 120ml immediately in the sawdust medium matter of both ends.If above-mentioned processing fails to make former base face
Color changes, and should continue to fill the water, it is ensured that ganoderma lucidum normal growth needs.
When former base is gradually differentiated to form cap, and after carrying out horizontal cross growth, to be determined according to cap edge color
Whether to continue to spray water.If cap edge, secondary side edge are respectively cream-coloured, ecru, water spray is not had to temporarily；If cap edge is
(shallow) yellow, then should spray water；If the color at cap edge is buff, increase the water spray frequency.
When ganoderma lucidum cap size is close or meets the market demand, the water spray frequency should be gradually decreased until stopping water spray.
7. ganoderma lucidum harvests.
After ganoderma lucidum maturation and edge thickness reaches 1cm or more and stops growing, and can harvest at this time.Under ganoderma lucidum harvesting
After coming, it should dry or dry in time, make its water content below 13%.It is put and is stored in a cool and dry place.
It is wherein used for the bacteria stick substance 20 jin/stick of average out to of cultivation, the nutritional ingredient in matrix to be compared with horn of plenty, in a life
Producing can not consume in the period, so remaining nutritional ingredient is also enough to tie up in matrix after the harvesting of First Year ganoderma lucidum maturation
Hold the normal growth and development of second year ganoderma lucidum.
8. second year ganoderma lucidum regrows development
When second year Clear and Bright, continuously turn to warm up in weather, temperature it is stable at 15 DEG C or more when, start in bacteria stick both ends sawdust
Middle injection 150ml or so clean cold water promotes mycelia in bacteria stick to sprout growth.Water filling 1 time daily, continuous water filling 3-4 times.It connects
Every 1-2 days fill the water 1 time, it is 5-6 times continuous.Every time after water filling, the film of arched shed above every furrow is put down, is closed, with
Promote ganoderma lucidum mycelium temperature needed for growth and development in bacteria stick.After former base is formed, according to the color of former base, water filling is determined
Amount is (according to step 6).Next management is carried out according to First Year step 6, step 7.
9. the cultivation of third year ganoderma lucidum
It is operated by step 1-7.
10. ganoderma lucidum regrowed development in the 4th year
It is operated by step 8.
Here is the result of test in continuous 4 years on same plot.
The annual per unit area yield of the not de- bag cultivating of 1 continuous 4 year ganoderma lucidum of table compares
*: go out sesame rate=go out sesame bacteria stick number/soil covering culture bacteria stick sum * 100%；
*: the weight of every produced dry glossy ganoderma of 100kg timber, unit are as follows: kg/100kg timber are indicated.
Table 1 statistics indicate that, bacteria stick go out four Nian Liwu conspicuousness of sesame rate variation；Table 2 statistics indicate that 1 year
Total per unit area yield and total per unit area yield of third and fourth year are also without substantial variation.
Yield comparison in the case of 2 continuous cropping of table
#: unit is kg/100kg timber.
Claims (3)
1. a kind of only carpogenic ganoderma lucidum cultivation method, it is characterised in that this method comprises the following steps:
The selection of cultivation area；The selection of cultivation of glossy ganoderma matrix；High-quality lucidum strain is selected；The production of ganoderma lucidum bacteria stick；Ganoderma lucidum is not
De- bag soil covering culture；Water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process；Ganoderma lucidum harvesting；
Wherein cultivation of glossy ganoderma matrix is juggle and sawdust materials.
2. only carpogenic ganoderma lucidum cultivation method according to claim 1, it is characterised in that this method includes following step
It is rapid:
(1) the selection of cultivation area: cultivation area is far from highway, linear distance 500m or more；
(2) cultivation matrix selects: juggle 25-35cm；Compost is sawdust materials；
(3) high-quality lucidum strain is selected: ganoderma strain being inoculated on PDA plate or similar solid medium, culture 4- is protected from light
6 days, the bacterium colony of formation was that rounding, mycelium are sturdy and fine and close, as more excellent strain；
(4) the production of ganoderma lucidum bacteria stick:
I, pack and sterilizing
Juggle is packed, fills sawdust materials, sterilizing at juggle both ends；
II, inoculation and culture
Cultivar is seeded on the sawdust materials at juggle both ends；Carry out stacking culture；
(5) ganoderma lucidum does not take off bag soil covering culture:
I, greenhouse is built
In the bacteria stick earthing previous moon, the soil for the cultivation area selected in step (1) is turned over, furrow are built；Every two furrow are built
The vinyl house of 1.8-2.2m high, is covered with plastic film above；
The discharge of II, bacteria stick and earthing
After ganoderma lucidum bacteria stick is trained ripe, it is transported to the arranged cultivation area put in order, not slough the bag on surface
It is directly placed on ground furrow in horizontal or vertical mode, and is covered with fine earth by son；If bacteria stick be it is horizontal,
Guarantee that the upper space of bacteria stick is just exposed；If vertical, then to ensure that one end of bacteria stick is just exposed；
(6) water storage, water filling and water spray moisturizing in ganoderma lucidum growth and development process:
After bacteria stick earthing, irrigation canals and ditches, wide 30-35cm, deep 40-60cm should be held successfully in canopy；Maintain have water in irrigation canals and ditches；
Bacteria stick earthing is after 10-15 days, and 1. bacteria stick is vertical, cuts along sack tether, but not strut sack；Or 2. bacteria stick
To be horizontal, then upper 1-2 small opennings are opened on the face that bacteria stick exposes earth's surface；
After forming former base, the water for injecting how many volume is judged according to the color of former base；
When former base is differentiated to form cap, and after carrying out horizontal cross growth, to determine whether to spray according to cap edge color
Water；
(7) ganoderma lucidum fruitbody harvests: after ganoderma lucidum maturation and edge thickness is more than that 1cm can be harvested.
3. according to claim 1 or 2 described in any item only carpogenic ganoderma lucidum cultivation methods, the second year after ganoderma lucidum harvesting
Mid or late March, temperature are stablized at 10-15 DEG C or more, inject water in the sawdust of bacteria stick both ends, and mycelia is promoted in bacteria stick to sprout life
It is long；After former base is formed, according to the color of former base, the amount of water filling is determined；When former base is differentiated to form cap, and carry out horizontal cross
After growth, to determine whether to spray water according to cap edge color；After ganoderma lucidum maturation and edge thickness is more than that 1cm can be adopted
It receives.
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|---|---|---|---|
| CN201811573573.8A CN109548559B (en) | 2018-12-21 | 2018-12-21 | Ganoderma lucidum cultivation method only producing fruiting bodies |
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| CN201811573573.8A CN109548559B (en) | 2018-12-21 | 2018-12-21 | Ganoderma lucidum cultivation method only producing fruiting bodies |
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| CN109548559B CN109548559B (en) | 2021-04-27 |
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| CN112913575A (en) * | 2021-03-04 | 2021-06-08 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma lucidum micropore bag opening and soil covering planting method |
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