CN109477128A - 在重组宿主中甜菊醇糖苷的生产 - Google Patents

在重组宿主中甜菊醇糖苷的生产 Download PDF

Info

Publication number
CN109477128A
CN109477128A CN201780030752.4A CN201780030752A CN109477128A CN 109477128 A CN109477128 A CN 109477128A CN 201780030752 A CN201780030752 A CN 201780030752A CN 109477128 A CN109477128 A CN 109477128A
Authority
CN
China
Prior art keywords
polypeptide
seq
amino acid
acid sequence
steviol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780030752.4A
Other languages
English (en)
Chinese (zh)
Inventor
艾弗·克拉夫斯·里谢德·汉森
韦罗尼克·杜尚
延斯·霍顿-拉森
埃斯本·哈尔克谢尔·汉森
安格利卡·塞姆莱尔
劳拉·奥基平蒂
基姆·奥尔森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evolva Holding SA
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of CN109477128A publication Critical patent/CN109477128A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Saccharide Compounds (AREA)
CN201780030752.4A 2016-05-16 2017-05-16 在重组宿主中甜菊醇糖苷的生产 Pending CN109477128A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662337213P 2016-05-16 2016-05-16
US62/337,213 2016-05-16
PCT/EP2017/061774 WO2017198681A1 (en) 2016-05-16 2017-05-16 Production of steviol glycosides in recombinant hosts

Publications (1)

Publication Number Publication Date
CN109477128A true CN109477128A (zh) 2019-03-15

Family

ID=58739035

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780030752.4A Pending CN109477128A (zh) 2016-05-16 2017-05-16 在重组宿主中甜菊醇糖苷的生产

Country Status (9)

Country Link
US (2) US20190144907A1 (https=)
EP (1) EP3458598A1 (https=)
JP (1) JP2019519212A (https=)
CN (1) CN109477128A (https=)
AU (1) AU2017267214A1 (https=)
BR (1) BR112018073662A2 (https=)
CA (1) CA3023399A1 (https=)
SG (1) SG11201809483UA (https=)
WO (1) WO2017198681A1 (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235124A (zh) * 2020-01-19 2020-06-05 云南农业大学 珠子参糖基转移酶UGTPjm2及其在制备竹节参皂苷IVa上的应用
CN112760301A (zh) * 2019-11-01 2021-05-07 中国科学院天津工业生物技术研究所 一种催化活性提高的糖基转移酶突变体及其应用
CN113308447A (zh) * 2021-05-31 2021-08-27 西南大学 拟南芥ugt74f2在催化苯乳酸合成苯乳酰葡萄糖中的应用
CN114736887A (zh) * 2022-03-25 2022-07-12 上海威高医疗技术发展有限公司 羧酸酯酶的用途
CN120758477A (zh) * 2025-09-10 2025-10-10 山东三元生物科技股份有限公司 一种糖基转移酶突变体及其在合成莱鲍迪苷m中的应用

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3497222T (pt) 2016-08-12 2022-02-09 Amyris Inc Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos
BR112019007504B1 (pt) * 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
BR112020009838A2 (pt) * 2017-12-05 2020-11-17 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
CA3101651A1 (en) * 2018-06-08 2019-12-12 Purecircle Usa Inc. High-purity steviol glycosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110564658B (zh) * 2019-09-06 2021-08-17 广西大学 一株大肠杆菌工程菌及其全细胞催化生产甜菊醇的方法
US11396646B2 (en) 2020-05-29 2022-07-26 QTG Development, Inc. Steviol glycosyltransferases and genes encoding the same
WO2025238041A1 (en) * 2024-05-16 2025-11-20 Evonik Operations Gmbh A novel rhamnolipid and glucolipid

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103732753A (zh) * 2011-08-08 2014-04-16 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
WO2015132411A2 (en) * 2014-03-07 2015-09-11 Evolva Sa Methods for recombinant production of saffron compounds
CN105051195A (zh) * 2013-02-06 2015-11-11 埃沃尔瓦公司 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN105671108A (zh) * 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009034080A (ja) * 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
BR112014012543A2 (pt) * 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
EP2929043A1 (en) * 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
US20160215306A1 (en) * 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
CN104845990A (zh) * 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105671108A (zh) * 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CN103732753A (zh) * 2011-08-08 2014-04-16 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN105051195A (zh) * 2013-02-06 2015-11-11 埃沃尔瓦公司 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法
WO2015132411A2 (en) * 2014-03-07 2015-09-11 Evolva Sa Methods for recombinant production of saffron compounds
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HUSAR, S等: "Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana", 《BMC PLANT BIOLOGY》 *
WANG, JF等: "Pathway mining-based integration of critical enzyme parts for de novo biosynthesis of steviolglycosides sweetener in Escherichia coli", 《CELL RESEARCH》 *
WANG, Y等: "Efficient enzymatic production of rebaudioside A from stevioside", 《BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112760301A (zh) * 2019-11-01 2021-05-07 中国科学院天津工业生物技术研究所 一种催化活性提高的糖基转移酶突变体及其应用
CN112760301B (zh) * 2019-11-01 2023-01-17 中国科学院天津工业生物技术研究所 一种催化活性提高的糖基转移酶突变体及其应用
CN111235124A (zh) * 2020-01-19 2020-06-05 云南农业大学 珠子参糖基转移酶UGTPjm2及其在制备竹节参皂苷IVa上的应用
CN111235124B (zh) * 2020-01-19 2023-04-07 云南农业大学 珠子参糖基转移酶UGTPjm2及其在制备竹节参皂苷IVa上的应用
CN113308447A (zh) * 2021-05-31 2021-08-27 西南大学 拟南芥ugt74f2在催化苯乳酸合成苯乳酰葡萄糖中的应用
CN113308447B (zh) * 2021-05-31 2022-09-30 西南大学 拟南芥ugt74f2在催化苯乳酸合成苯乳酰葡萄糖中的应用
CN114736887A (zh) * 2022-03-25 2022-07-12 上海威高医疗技术发展有限公司 羧酸酯酶的用途
CN120758477A (zh) * 2025-09-10 2025-10-10 山东三元生物科技股份有限公司 一种糖基转移酶突变体及其在合成莱鲍迪苷m中的应用
CN120758477B (zh) * 2025-09-10 2025-11-18 山东三元生物科技股份有限公司 一种糖基转移酶突变体及其在合成莱鲍迪苷m中的应用

Also Published As

Publication number Publication date
BR112018073662A2 (pt) 2019-02-19
US20220154234A1 (en) 2022-05-19
CA3023399A1 (en) 2017-11-23
SG11201809483UA (en) 2018-11-29
AU2017267214A1 (en) 2018-11-15
US20190144907A1 (en) 2019-05-16
WO2017198681A1 (en) 2017-11-23
JP2019519212A (ja) 2019-07-11
EP3458598A1 (en) 2019-03-27

Similar Documents

Publication Publication Date Title
CN109477128A (zh) 在重组宿主中甜菊醇糖苷的生产
EP3387136B1 (en) Production of steviol glycosides in recombinant hosts
CN107109358B (zh) 在重组宿主中生产甜菊醇糖苷
EP3332018B1 (en) Production of steviol glycosides in recombinant hosts
CN108337892B (zh) 在重组宿主中生产甜菊醇糖苷
CN105189771B (zh) 在重组宿主中有效产生甜菊醇糖苷
US10947515B2 (en) UDP-glycosyltransferases
CN106572688B (zh) 在重组宿主中生产甜菊醇糖苷
US20150342234A1 (en) Steviol Glycoside Compositions Sensory Properties
US10815514B2 (en) Production of steviol glycosides in recombinant hosts
CN111566222A (zh) 在重组宿主中产生甜菊醇糖苷
US11396669B2 (en) Production of steviol glycosides in recombinant hosts
CN109195457A (zh) 在重组宿主中产生甜菊醇糖苷
CN109154010A (zh) 在重组宿主中生产甜菊醇糖苷

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20190315

WD01 Invention patent application deemed withdrawn after publication