CA3023399A1 - Production of steviol glycosides in recombinant hosts - Google Patents

Production of steviol glycosides in recombinant hosts Download PDF

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Publication number
CA3023399A1
CA3023399A1 CA3023399A CA3023399A CA3023399A1 CA 3023399 A1 CA3023399 A1 CA 3023399A1 CA 3023399 A CA3023399 A CA 3023399A CA 3023399 A CA3023399 A CA 3023399A CA 3023399 A1 CA3023399 A1 CA 3023399A1
Authority
CA
Canada
Prior art keywords
polypeptide
seq
set forth
amino acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3023399A
Other languages
English (en)
French (fr)
Inventor
Iver Klavs Riishede HANSEN
Veronique Douchin
Jens Houghton-Larsen
Esben Halkjaer Hansen
Angelika SEMMLER
Laura OCCHIPINTI
Kim OLSSON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evolva Holding SA
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of CA3023399A1 publication Critical patent/CA3023399A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Saccharide Compounds (AREA)
CA3023399A 2016-05-16 2017-05-16 Production of steviol glycosides in recombinant hosts Pending CA3023399A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662337213P 2016-05-16 2016-05-16
US62/337,213 2016-05-16
PCT/EP2017/061774 WO2017198681A1 (en) 2016-05-16 2017-05-16 Production of steviol glycosides in recombinant hosts

Publications (1)

Publication Number Publication Date
CA3023399A1 true CA3023399A1 (en) 2017-11-23

Family

ID=58739035

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3023399A Pending CA3023399A1 (en) 2016-05-16 2017-05-16 Production of steviol glycosides in recombinant hosts

Country Status (9)

Country Link
US (2) US20190144907A1 (https=)
EP (1) EP3458598A1 (https=)
JP (1) JP2019519212A (https=)
CN (1) CN109477128A (https=)
AU (1) AU2017267214A1 (https=)
BR (1) BR112018073662A2 (https=)
CA (1) CA3023399A1 (https=)
SG (1) SG11201809483UA (https=)
WO (1) WO2017198681A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3497222T (pt) 2016-08-12 2022-02-09 Amyris Inc Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos
BR112019007504B1 (pt) * 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
BR112020009838A2 (pt) * 2017-12-05 2020-11-17 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
CA3101651A1 (en) * 2018-06-08 2019-12-12 Purecircle Usa Inc. High-purity steviol glycosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110564658B (zh) * 2019-09-06 2021-08-17 广西大学 一株大肠杆菌工程菌及其全细胞催化生产甜菊醇的方法
CN112760301B (zh) * 2019-11-01 2023-01-17 中国科学院天津工业生物技术研究所 一种催化活性提高的糖基转移酶突变体及其应用
CN111235124B (zh) * 2020-01-19 2023-04-07 云南农业大学 珠子参糖基转移酶UGTPjm2及其在制备竹节参皂苷IVa上的应用
US11396646B2 (en) 2020-05-29 2022-07-26 QTG Development, Inc. Steviol glycosyltransferases and genes encoding the same
CN113308447B (zh) * 2021-05-31 2022-09-30 西南大学 拟南芥ugt74f2在催化苯乳酸合成苯乳酰葡萄糖中的应用
CN114736887A (zh) * 2022-03-25 2022-07-12 上海威高医疗技术发展有限公司 羧酸酯酶的用途
WO2025238041A1 (en) * 2024-05-16 2025-11-20 Evonik Operations Gmbh A novel rhamnolipid and glucolipid
CN120758477B (zh) * 2025-09-10 2025-11-18 山东三元生物科技股份有限公司 一种糖基转移酶突变体及其在合成莱鲍迪苷m中的应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009034080A (ja) * 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
EP2575432B1 (en) * 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
CA3128532A1 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
BR112014012543A2 (pt) * 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
EP2929043A1 (en) * 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
MY199452A (en) * 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US20160215306A1 (en) * 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
SG10201807693YA (en) * 2014-03-07 2018-10-30 Evolva Sa Methods for recombinant production of saffron compounds
CA2960693A1 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN104845990A (zh) * 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用

Also Published As

Publication number Publication date
BR112018073662A2 (pt) 2019-02-19
US20220154234A1 (en) 2022-05-19
SG11201809483UA (en) 2018-11-29
AU2017267214A1 (en) 2018-11-15
US20190144907A1 (en) 2019-05-16
WO2017198681A1 (en) 2017-11-23
JP2019519212A (ja) 2019-07-11
CN109477128A (zh) 2019-03-15
EP3458598A1 (en) 2019-03-27

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Effective date: 20220512