JP2019519212A - 組換え宿主内でのステビオールグリコシドの産生 - Google Patents

組換え宿主内でのステビオールグリコシドの産生 Download PDF

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Publication number
JP2019519212A
JP2019519212A JP2018560137A JP2018560137A JP2019519212A JP 2019519212 A JP2019519212 A JP 2019519212A JP 2018560137 A JP2018560137 A JP 2018560137A JP 2018560137 A JP2018560137 A JP 2018560137A JP 2019519212 A JP2019519212 A JP 2019519212A
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Japan
Prior art keywords
polypeptide
seq
amino acid
acid sequence
set forth
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Pending
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JP2018560137A
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Japanese (ja)
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JP2019519212A5 (https=
Inventor
クラフス リイシェデ ハンセン，イヴァー
クラフス リイシェデ ハンセン，イヴァー
ドーキン，ヴェロニク
ホートン−ラーセン，ジェンス
ハールケーア ハンセン，エスベン
ハールケーア ハンセン，エスベン
セムラ—，アンジェリカ
セムラ―，アンジェリカ
オキピンティ，ローラ
オルソン，キム
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Evolva Holding SA
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Evolva AG
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Application filed by Evolva AG filed Critical Evolva AG
Publication of JP2019519212A publication Critical patent/JP2019519212A/ja
Publication of JP2019519212A5 publication Critical patent/JP2019519212A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Saccharide Compounds (AREA)
JP2018560137A 2016-05-16 2017-05-16 組換え宿主内でのステビオールグリコシドの産生 Pending JP2019519212A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662337213P 2016-05-16 2016-05-16
US62/337,213 2016-05-16
PCT/EP2017/061774 WO2017198681A1 (en) 2016-05-16 2017-05-16 Production of steviol glycosides in recombinant hosts

Publications (2)

Publication Number Publication Date
JP2019519212A true JP2019519212A (ja) 2019-07-11
JP2019519212A5 JP2019519212A5 (https=) 2020-06-25

Family

ID=58739035

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018560137A Pending JP2019519212A (ja) 2016-05-16 2017-05-16 組換え宿主内でのステビオールグリコシドの産生

Country Status (9)

Country Link
US (2) US20190144907A1 (https=)
EP (1) EP3458598A1 (https=)
JP (1) JP2019519212A (https=)
CN (1) CN109477128A (https=)
AU (1) AU2017267214A1 (https=)
BR (1) BR112018073662A2 (https=)
CA (1) CA3023399A1 (https=)
SG (1) SG11201809483UA (https=)
WO (1) WO2017198681A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3497222T (pt) 2016-08-12 2022-02-09 Amyris Inc Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos
BR112019007504B1 (pt) * 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
BR112020009838A2 (pt) * 2017-12-05 2020-11-17 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
CA3101651A1 (en) * 2018-06-08 2019-12-12 Purecircle Usa Inc. High-purity steviol glycosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110564658B (zh) * 2019-09-06 2021-08-17 广西大学 一株大肠杆菌工程菌及其全细胞催化生产甜菊醇的方法
CN112760301B (zh) * 2019-11-01 2023-01-17 中国科学院天津工业生物技术研究所 一种催化活性提高的糖基转移酶突变体及其应用
CN111235124B (zh) * 2020-01-19 2023-04-07 云南农业大学 珠子参糖基转移酶UGTPjm2及其在制备竹节参皂苷IVa上的应用
US11396646B2 (en) 2020-05-29 2022-07-26 QTG Development, Inc. Steviol glycosyltransferases and genes encoding the same
CN113308447B (zh) * 2021-05-31 2022-09-30 西南大学 拟南芥ugt74f2在催化苯乳酸合成苯乳酰葡萄糖中的应用
CN114736887A (zh) * 2022-03-25 2022-07-12 上海威高医疗技术发展有限公司 羧酸酯酶的用途
WO2025238041A1 (en) * 2024-05-16 2025-11-20 Evonik Operations Gmbh A novel rhamnolipid and glucolipid
CN120758477B (zh) * 2025-09-10 2025-11-18 山东三元生物科技股份有限公司 一种糖基转移酶突变体及其在合成莱鲍迪苷m中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013533736A (ja) * 2010-06-02 2013-08-29 エボルバ ニュートリション， インク． ステビオールグリコシドの組換え生成
JP2015536157A (ja) * 2012-12-04 2015-12-21 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． モグロシド化合物の生合成のための方法および材料
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009034080A (ja) * 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
CA3128532A1 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
BR112014012543A2 (pt) * 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
MY199452A (en) * 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US20160215306A1 (en) * 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
SG10201807693YA (en) * 2014-03-07 2018-10-30 Evolva Sa Methods for recombinant production of saffron compounds
CN104845990A (zh) * 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013533736A (ja) * 2010-06-02 2013-08-29 エボルバ ニュートリション， インク． ステビオールグリコシドの組換え生成
JP2015536157A (ja) * 2012-12-04 2015-12-21 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． モグロシド化合物の生合成のための方法および材料
WO2016038095A2 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BIOSCIENCE,BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 80, no. 1, JPN6021023924, 2 January 2016 (2016-01-02), pages 67 - 73, ISSN: 0004692070 *
CELL RESEARCH, vol. 26, JPN6021023927, 11 September 2015 (2015-09-11), pages 258 - 261, ISSN: 0004692069 *

Also Published As

Publication number Publication date
BR112018073662A2 (pt) 2019-02-19
US20220154234A1 (en) 2022-05-19
CA3023399A1 (en) 2017-11-23
SG11201809483UA (en) 2018-11-29
AU2017267214A1 (en) 2018-11-15
US20190144907A1 (en) 2019-05-16
WO2017198681A1 (en) 2017-11-23
CN109477128A (zh) 2019-03-15
EP3458598A1 (en) 2019-03-27

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