CN109463233B - Method for quickly cultivating large seedlings of panax japonicus - Google Patents
Method for quickly cultivating large seedlings of panax japonicus Download PDFInfo
- Publication number
- CN109463233B CN109463233B CN201811327117.5A CN201811327117A CN109463233B CN 109463233 B CN109463233 B CN 109463233B CN 201811327117 A CN201811327117 A CN 201811327117A CN 109463233 B CN109463233 B CN 109463233B
- Authority
- CN
- China
- Prior art keywords
- seedling
- culture medium
- root
- germination
- accelerating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for quickly cultivating a large seedling of panax japonicus, which comprises the following steps: (1) selecting rhizoma Panacis Majoris to culture seed-setting seedling; (2) cutting off the stichopus japonicus cultured seed-setting big seedlings into a plurality of sections of cultured seedling root sections; (3) pre-treating the root section of the cultivated seedling: soaking the raw materials in a first bactericide and cytokinin in sequence, and then airing the raw materials for later use; (4) accelerating germination: building a germination accelerating pool indoors, putting the cultured seedling root sections into the germination accelerating pool, and germinating the sprouts to form germination accelerating root sections; (5) and (3) accelerating germination and treating root segments: soaking the seeds in a second bactericide, then soaking the seeds in rooting powder, and airing the seeds for later use; (6) seedling culture: correspondingly embedding the germination accelerating root sections in the step (5) into seedling raising trays, and then covering a culture medium; (7) building a sunshade net; (8) managing after seedling culture: the culture medium is watered regularly, the insecticide is sprayed regularly, and the stichopus japonicus is transplanted after being cultivated until the stichopus japonicus seedlings are grown, so that the aim of shortening the growth period of the stichopus japonicus on the basis of ensuring higher breeding coefficient is fulfilled.
Description
Technical Field
The invention belongs to the technical field of planting of traditional Chinese medicinal materials, and particularly relates to a rapid cultivation method of large seedlings of panax japonicus.
Background
The Panax japonicum is Panax japonicus of Araliaceae, belonging to Panax japonicum (Panax japonicum wall. var. major (Burkill) H.L.Li) and Panax notoginseng (Panax bipinnatifidus Seem.) dried rhizome, with slender rhizome, expanded section into spheroidal shape, and more in the shape of cluster pimple, and fine adventitious root beside the side.
The traditional Chinese medicine considers that: rhizoma Panacis Majoris has bitter, sweet and slightly cold nature, and has effects of invigorating lung, nourishing yin, activating collaterals, and stopping bleeding, and can be used for treating deficiency of both qi and yin, dysphoria, thirst, cough due to asthenia, traumatic injury, arthralgia, hemoptysis, hematemesis, and traumatic hemorrhage; the main chemical components of the rhizoma panacis majoris are saponins, and at present, 25 saponins are co-separated, such as oleanane type saponins represented by panax japonicus saponins and the like; dammarane type saponin represented by ginsenoside, and Okottama type saponin represented by rhizoma Panacis Majoris saponin. The saponins may not be the only effective component of rhizoma Panacis Majoris, and contain active polysaccharide, multiple microelements, etc. besides saponins. The main effects currently known are as follows: has obvious antitumor effect, protecting cardiac and cerebral vascular diseases, resisting inflammation, relieving pain, enhancing immunity, and protecting hemopoietic function and blood.
Currently, the rhizoma panacis majoris realizes the artificial breeding of seeds and rhizomes, and comprises seed source selection and treatment, sowing or planting, land selection, land preparation, bed making, shade shed building, field management and pest control. However, since the panax japonicus grows slowly, more than 6 years are needed from the seeds to the digging of the commodities, wherein about 4 years are needed from the seeds to the flowering and the fructification, the panax japonicus seed grows slowly in the first three years, and the panax japonicus seed enters the rapid growth period after the third year. Therefore, many Panax japonicum planting farmers want to rapidly cultivate large seedlings capable of blooming and fructifying, so as to reduce the planting period of Panax japonicum and generate seeds as soon as possible.
Disclosure of Invention
In view of the above, in order to solve the above problems in the prior art, the present invention aims to provide a method for rapidly cultivating a large seedling of panax japonicus so as to achieve the purpose of shortening the growth cycle of the panax japonicus on the basis of ensuring a high breeding coefficient.
The technical scheme adopted by the invention is as follows: a method for quickly cultivating a large seedling of panax japonicus comprises the following steps:
(1) seed selection: selecting healthy and disease and insect pest-free rhizoma panacis majoris to cultivate fruiting large seedlings;
(2) cutting rhizomes into sections: cutting the stichopus japonicus cultured seed-setting seedling into a plurality of sections of cultured seedling root sections, wherein each section of cultured seedling root section at least comprises one swelling joint;
(3) pre-treating the root section of the cultivated seedling: soaking the root sections of each section of the cultivated seedlings with a first bactericide and cytokinin in sequence, and then airing for later use;
(4) accelerating germination: building a germination accelerating pool indoors, horizontally placing the cultured seedling root sections in the germination accelerating pool layer by layer, keeping the temperature in the germination accelerating pool at 20-25 ℃, and germinating for 15-20 days to form germination accelerating root sections;
(5) and (3) accelerating germination and treating root segments: soaking the seeds in a second bactericide, then soaking the seeds in rooting powder, and airing the seeds for later use;
(6) seedling culture: filling a culture medium into a seedling raising tray, correspondingly embedding the germination accelerating root section in the step (5) into the seedling raising tray, covering the culture medium, and placing the culture medium into cultivated land;
(7) building a sunshade net: building a sunshade net above the cultivated land;
(8) managing after seedling culture: watering the culture medium regularly, spraying insecticide regularly after seedling emergence, and transplanting after the seedling is grown until the seed-setting stichopus japonicus seedlings are grown.
Further, in the step (1), the seed selection time is within the period of 9 months to 10 months.
Further, in the step (1), the growth time of the stichopus japonicus breeding fructification big seedling is more than 4 years, and the diameter of the expansion joint is not less than 0.3 cm.
Further, in the step (2), the seedling root section comprises an expansion joint, and the bridge length from the expansion joint to the end part is 1-2 cm.
Further, in the step (3), the first bactericide is soaked for 2-3 minutes and then the cytokinin is soaked for 1.5-2.5 hours, and the first bactericide and the cytokinin are carbendazim solution and 2mg/L of 6-BA respectively.
Further, the specific steps in the step (4) are as follows:
1) building a rectangular germination accelerating pond indoors;
2) sun-dried straws with the thickness of 8-12cm are paved at the bottom and the periphery of the germination accelerating pond; then, rice bran is paved, and the thickness of the rice bran is 4-6 cm; laying 3-4 layers of straw paper;
3) laying the cultured seedling root sections above the straw paper layer by layer, wherein the stacking height of the cultured seedling root sections is 4-6cm, 8-12cm thick straw is laid above the stacked seedling root sections, and cotton quilts or cotton blankets are covered above the straw for heat preservation;
4) and keeping the temperature in the germination accelerating pool at 20-25 ℃, and forming a germination accelerating root section after the germination of the sprouts for 15-20 days.
Further, in the step (5), the second bactericide is soaked for 2-3 minutes and then the rooting powder is soaked for 0.8-1.2 hours, and the second bactericide and the rooting powder are the carbendazim solution and the ABT rooting powder of 900-fold 1100-liquid respectively.
Further, in the step (6), the culture medium is forest humus soil, and the pretreatment method of the culture medium is as follows:
a) spraying the culture medium with 900-fold carbendazim diluent of 1100 times to make the water content reach 60-70%;
b) the film is used after being covered for 3-4 days for disinfection and sterilization, and the water content of the culture medium is kept at 55-65%.
Further, in the step (6), the seedling raising tray is provided with water leakage holes, the caliber of the seedling raising tray is greater than or equal to 6cm, and the hole depth is greater than or equal to 5 cm.
Further, in the step (8), the watering period of the culture medium is 1-3 days, and the water content of the culture medium is kept at 55-65%; the pesticide is prepared by 800 times of amobam liquid and 1000 times of dimethoate, and the spraying period of the pesticide is 10-15 days.
The invention has the beneficial effects that:
1. according to the rapid cultivation method of the large pearl ginseng seedlings, provided by the invention, the germination percentage and the survival rate of the single-section cultivated seedling root section of the pearl ginseng in the cultivation process are greatly improved through the germination accelerating treatment and the two-time disinfection growth promoting treatment (namely, the pretreatment of the cultivated seedling root section and the germination accelerating root section treatment), the 6-year growth period of the seed seedling of the pearl ginseng reaching the medicinal material digging specification can be shortened to 3-4 years, and the flowering and fruiting time of the seed seedling of the pearl ginseng is shortened to about 1 year from 3-4 years.
2. According to the rapid cultivation method of the stichopus japonicus big seedling, provided by the invention, the steps of pretreatment of the root section of the cultivated seedling, treatment of the germination accelerating root section, treatment of the culture medium and management after seedling cultivation are adopted, so that the higher propagation coefficient of about 4-6 can be kept on the premise of greatly shortening the seedling cultivation and growth period, and the method is an efficient, reliable, simple and convenient way for obtaining the stichopus japonicus big seedling at present.
3. According to the rapid cultivation method of the panax japonicus big seedlings, segmented rhizomes of the panax japonicus are used as an original breeding material, and strong flowering and fruiting panax japonicus big seedlings are cultivated within 1 year on the basis of ensuring high breeding coefficients through the technologies of rhizome cutting, pretreatment sterilization of root segments of cultivated seedlings, germination acceleration, pretreatment of culture mediums, treatment of germination acceleration root segments, seedling cultivation, sunshade net building, management after seedling cultivation and the like.
Detailed Description
For clear and complete description of the technical solutions in the embodiments of the present invention, it is obvious that the described embodiments are a part of the embodiments of the present invention, and not all embodiments.
Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
It should be noted that: in the description of the embodiments of the present invention, the terms "first" and "second" are used merely to distinguish descriptions, and are not intended to indicate or imply relative importance.
The first embodiment is as follows:
in this embodiment, a method for rapidly breeding a large seedling of panax japonicus is specifically provided, and the steps of the breeding method are specifically as follows:
(1) seed selection: selecting healthy and disease and insect pest-free rhizoma panacis majoris to cultivate fruiting large seedlings; the seed selection time is 9 months early per year, the growth time of the stichopus japonicus breeding fructification big seedling is more than 4 years, and the diameter of an expansion joint is not less than 0.3 cm.
(2) Cutting rhizomes into sections: cutting the large seedlings of the stichopus japonicus culture fructification into a plurality of sections of seedling root sections, and ensuring that each section of seedling root section comprises a swelling section. The specific treatment method comprises the following steps: the method comprises the steps of cutting a strong seedling cultivated by the pearl ginseng along the space between an expansion joint and another adjacent expansion joint by a knife (namely, cutting a gap bridge between the two adjacent expansion joints), wherein generally, one expansion joint is used as a root section of one cultivated seedling, only about 1-2cm of gap bridge can be reserved if the gap bridge between two adjacent expansion joints is too long, and in the embodiment, the gap bridge length from the expansion joint to the end is 1 cm.
(3) Pre-treating the root section of the cultivated seedling: soaking the root section of each seedling culture section in a carbendazim solution for 2 minutes, then soaking the root section in 2 mg/L6-BA cytokinin for 1.5 hours, and airing for later use. In the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the 6-BA cytokinin can also be replaced by cytokinin such as Kinetin (KT) or Zeatin (ZT).
(4) Accelerating germination: 1) building a rectangular germination accelerating pond by using adobes indoors, wherein the height of the pond wall is 60 cm, and the length and the width of the pond wall are determined according to the number of root sections;
2) before the root segment of the cultured seedling is placed, a layer of dried straw is paved at the bottom and the periphery of the germination accelerating pool, and the thickness of the straw is 8 cm; laying a layer of bran coat, wherein the thickness of the bran coat is 4 cm; laying 3 layers of straw paper;
3) when the temperature of the cultivated seedling root section is slightly high in sunny and warm weather, the cultivated seedling root section is horizontally placed above grass paper layer by layer, the stacking height of the cultivated seedling root section is 4cm, rice straws with the thickness of 8cm are paved above the stacked rice straws, and cotton quilts are covered above the rice straws to cover the pool for heat preservation;
4) keeping the temperature in the germination accelerating pool at 20 ℃, and forming a germination accelerating root section after the germination of the sprouts for 15 days, thus preparing for seedling cultivation in the ground.
(5) And (3) accelerating germination and treating root segments: soaking in carbendazim solution for 2 min, soaking in 900 times ABT rooting powder for 0.8 hr, and air drying; in the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the ABT rooting powder can also be replaced by APT rooting powder or strong rooting powder.
(6) Seedling culture:
selecting forest humus as a culture medium, wherein the pretreatment method of the culture medium comprises the following steps:
a) spraying the culture medium with 900 times carbendazim diluent to reach water content of 60%;
b) covering with film for 3 days, sterilizing, and maintaining the water content of culture medium at 55%;
II, filling a culture medium into a seedling raising tray by 1.8cm, burying the germination accelerating root section in the step (5) into the seedling raising tray by one plant in one hole, covering the culture medium, wherein the thickness of the culture medium covering the germination accelerating root section is 1cm, placing the culture medium in a farmland which is leveled, weeding and free of water accumulation, and placing the culture medium according to the seedling raising tray with a width of 0.5m left about 1.2 m; wherein, the seedling raising tray is provided with water leakage holes (namely adopting seedling raising seedlings with the water leakage holes), the caliber of the seedling raising tray is selected to be 6cm, and the hole depth is selected to be 5 cm.
(7) Building a sunshade net: the sunshade net is built above the cultivated land, and the method comprises the following specific steps: a sunshade net rack with the height of 1.5m is built by bamboo wood, the sunshade net is covered fully, and the sunshade rate is 65%;
(8) managing after seedling culture: when seedling is raised, the watering period of the culture medium is 1 day and the water content of the culture medium is kept at 65 percent; spraying 800 times of amobam liquid and 1000 times of dimethoate as pesticides, wherein the interval period of the pesticides is 10 days; after seedling growing, the seedlings can be transplanted as seed-setting stichopus japonicus big seedlings after being cultivated to 9 months in the current year, and the seedling growing time can be controlled to be about 1 year. In this step, the dilution ratio of the ammonium senate solution and the dimethoate is only a preferred technical solution in this embodiment, and may also be adjusted according to actual situations, which is not specifically exemplified herein.
The second embodiment is as follows:
in this embodiment, a method for rapidly breeding a large seedling of panax japonicus is specifically provided, and the steps of the breeding method are specifically as follows:
(1) seed selection: selecting healthy and disease and insect pest-free rhizoma panacis majoris to cultivate fruiting large seedlings; the seed selection time is 9 middle ten days per year, the growth time of the stichopus japonicus breeding fructification big seedling is more than 4 years, and the diameter of an expansion node is not less than 0.3 cm.
(2) Cutting rhizomes into sections: cutting the large seedlings of the stichopus japonicus culture fructification into a plurality of sections of seedling root sections, and ensuring that each section of seedling root section comprises a swelling section. The specific treatment method comprises the following steps: the method comprises the steps of cutting a seed-grown seedling cultured by the pearl ginseng along a gap between an expanded joint and another adjacent expanded joint by using a knife (namely, cutting a gap bridge between the seed-grown seedling cultured by the pearl ginseng), wherein generally, one expanded joint is used as a root section of one cultured seedling, only about 1-2cm of gap bridge can be reserved if the gap bridge between two adjacent expanded joints is too long, and in the embodiment, the gap bridge length from the expanded joint to the end is 1.5 cm.
(3) Pre-treating the root section of the cultivated seedling: soaking the root section of each seedling culture section in a carbendazim solution for 2.5 minutes, then soaking the root section in 2 mg/L6-BA cytokinin for 2 hours, and airing for later use. In the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the 6-BA cytokinin can also be replaced by cytokinin such as Kinetin (KT) or Zeatin (ZT).
(4) Accelerating germination: 1) building a rectangular germination accelerating pond by using adobes indoors, wherein the height of the wall of the germination accelerating pond is 80 cm, and the length and the width of the germination accelerating pond are determined according to the number of root sections;
2) before the root section of the cultured seedling is placed, a layer of dried straw is paved at the bottom and the periphery of the germination accelerating pool, and the thickness of the straw is 10 cm; laying a layer of bran coat, wherein the thickness of the bran coat is 5 cm; laying 4 layers of straw paper;
3) when the temperature of the cultivated seedling root section is slightly high in sunny and warm weather, the cultivated seedling root section is horizontally placed above grass paper layer by layer, the stacking height of the cultivated seedling root section is 5cm, 10 cm-thick straw is paved above the stacked seedling root section, and cotton blankets are covered above the straw to cover the pool for heat preservation;
4) keeping the temperature in the germination accelerating pool at 22 ℃, and forming a germination accelerating root section after 18 days of germination of the sprouts so as to prepare for underground seedling culture.
(5) And (3) accelerating germination and treating root segments: soaking in carbendazim solution for 3 min, soaking in 1000 times ABT rooting powder for 1 hr, and air drying; in the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the ABT rooting powder can also be replaced by APT rooting powder or strong rooting powder.
(6) Seedling culture:
selecting forest humus as a culture medium, wherein the pretreatment method of the culture medium comprises the following steps:
a) spraying the culture medium with 1000 times of carbendazim diluent to make the water content reach 65%;
b) covering with film for 3.5 days, sterilizing, and maintaining the water content of culture medium at 60%;
II, filling a culture medium into a seedling raising tray by 2cm, burying the germination accelerating root section in the seedling raising tray in the step (5) by one plant according to one hole, covering the culture medium, wherein the thickness of the culture medium covering the germination accelerating root section is 1.5cm, placing the culture medium in a farmland which is leveled, weeding and free of water accumulation, and placing the culture medium according to the seedling raising tray with a width of 0.5m left about 1.2 m; wherein, the seedling raising tray is provided with water leakage holes (namely adopting the seedling raising seedlings with the water leakage holes), the caliber of the seedling raising tray is 8cm, and the hole depth is 6 cm.
(7) Building a sunshade net: the sunshade net is built above the cultivated land, and the method comprises the following specific steps: a sunshade net rack with the height of 2m is built by bamboo wood, and the sunshade net is covered fully, so that the sunshade rate is 70%;
(8) managing after seedling culture: when seedling is raised, the watering period of the culture medium is 2 days and the water content of the culture medium is kept at 60%; spraying 800 times of amobam liquid and 1000 times of dimethoate as pesticides, wherein the interval period of the pesticides is 12 days; after seedling, the seedlings are cultivated until 9 middle days of the year, and then the seedlings can be transplanted as big seedlings of the stichopus japonicus, and the seedling emergence time can be controlled to be about 1 year. In this step, the dilution ratio of the ammonium senate solution and the dimethoate is only a preferred technical solution in this embodiment, and may also be adjusted according to actual situations, which is not specifically exemplified herein.
The third concrete embodiment:
in this embodiment, a method for rapidly breeding a large seedling of panax japonicus is specifically provided, and the steps of the breeding method are specifically as follows:
(1) seed selection: selecting healthy and disease and insect pest-free rhizoma panacis majoris to cultivate fruiting large seedlings; the seed selection time is 10 months early per year, the growth time of the stichopus japonicus breeding fructification big seedling is more than 4 years, and the diameter of an expansion joint is not less than 0.3 cm.
(2) Cutting rhizomes into sections: cutting the large seedlings of the stichopus japonicus culture fructification into a plurality of sections of seedling root sections, and ensuring that each section of seedling root section comprises a swelling section. The specific treatment method comprises the following steps: the method comprises the steps of cutting a strong seedling cultivated by the pearl ginseng along the space between an expansion joint and another adjacent expansion joint by a knife (namely, cutting a gap bridge between the two adjacent expansion joints), wherein generally, one expansion joint is used as a root section of one cultivated seedling, only about 1-2cm of gap bridge can be reserved if the gap bridge between the two adjacent expansion joints is too long, and in the embodiment, the gap bridge length from the expansion joint to the end is 2 cm.
(3) Pre-treating the root section of the cultivated seedling: soaking the root section of each seedling culture section in a carbendazim solution for 3 minutes, then soaking the root section in 2 mg/L6-BA cytokinin for 2.5 hours, and airing for later use. In the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the 6-BA cytokinin can also be replaced by cytokinin such as Kinetin (KT) or Zeatin (ZT).
(4) Accelerating germination: 1) building a rectangular germination accelerating pond indoors by using bricks, wherein the height of the pond wall is 70 cm, and the length and the width of the pond wall are determined according to the number of root sections;
2) before the root segment of the cultured seedling is placed, a layer of dried straw is paved at the bottom and the periphery of the germination accelerating pool, and the thickness of the straw is 12 cm; laying a layer of bran coat, wherein the thickness of the bran coat is 6 cm; laying 4 layers of straw paper;
3) when the temperature of the cultivated seedling root section is slightly high in sunny and warm weather, the cultivated seedling root section is horizontally placed above grass paper layer by layer, the stacking height of the cultivated seedling root section is 6cm, rice straws with the thickness of 12cm are paved above the stacked rice straws, and cotton quilts are covered above the rice straws to cover the pool for heat preservation;
4) keeping the temperature in the germination accelerating pool at 20 ℃, and forming a germination accelerating root section after the germination of the sprouts for 15 days, thus preparing for seedling cultivation in the ground.
(5) And (3) accelerating germination and treating root segments: soaking in carbendazim solution for 3 min, soaking in ABT rooting powder of 1100 times of solution for 1.2 hr, and air drying; in the step, the carbendazim solution can also be replaced by hymexazol solution or benomyl solution for sterilization treatment; the ABT rooting powder can also be replaced by APT rooting powder or strong rooting powder.
(6) Seedling culture:
selecting forest humus as a culture medium, wherein the pretreatment method of the culture medium comprises the following steps:
a) spraying the culture medium with 1100 times of carbendazim diluent to make the water content reach 70%;
b) covering with film for 4 days, sterilizing, and maintaining the water content of culture medium at 65%;
II, filling a culture medium into a seedling raising tray by 2.2cm, burying the germination accelerating root section in the seedling raising tray according to one hole and one strain, covering the culture medium, wherein the thickness of the culture medium covering the germination accelerating root section is 2cm, placing the culture medium in a farmland which is smooth, weeding and free of water accumulation, and placing the culture medium according to a seedling raising tray with a width of 0.6m left about 1.1 m; wherein, the seedling raising tray is provided with water leakage holes (namely adopting the seedling raising seedlings with the water leakage holes), the caliber of the seedling raising tray is 8cm, and the hole depth is 6 cm.
(7) Building a sunshade net: the sunshade net is built above the cultivated land, and the method comprises the following specific steps: a sunshade net rack with the height of 1.8m is built by bamboo wood, the sunshade net is covered fully, and the sunshade rate is 72 percent;
(8) managing after seedling culture: when seedling is raised, the watering period of the culture medium is 3 days and the water content of the culture medium is kept to be 55 percent; spraying 800 times of amobam liquid and 1000 times of dimethoate as pesticides, wherein the interval period of the pesticides is 15 days; after seedling growing, the seedlings can be transplanted as seed-setting stichopus japonicus big seedlings after being cultivated for 10 months in the current year, and the seedling growing time can be controlled to be about 1 year. In this step, the dilution ratio of the ammonium senate solution and the dimethoate is only a preferred technical solution in this embodiment, and may also be adjusted according to actual situations, which is not specifically exemplified herein.
In the three embodiments, compared with other technologies for growing stichopus japonicus, the germination rate and the survival rate of each section of cultured seedling root section (capable of keeping the propagation coefficient at about 5) are greatly improved by adopting the treatment mode through single germination accelerating treatment and twice disinfection and sterilization growth promoting treatment.
The invention is not limited to the above alternative embodiments, and any other various forms of products can be obtained by anyone in the light of the present invention, but any changes in shape or structure thereof, which fall within the scope of the present invention as defined in the claims, fall within the scope of the present invention.
Claims (7)
1. A method for quickly cultivating a large seedling of panax japonicus is characterized by comprising the following steps:
(1) seed selection: selecting healthy and disease and insect pest-free rhizoma panacis majoris to cultivate fruiting large seedlings;
(2) cutting rhizomes into sections: cutting the stichopus japonicus cultured seed-setting seedling into a plurality of sections of cultured seedling root sections, wherein each section of cultured seedling root section at least comprises one swelling joint;
(3) pre-treating the root section of the cultivated seedling: soaking the root sections of each section of the cultivated seedlings with a first bactericide and cytokinin in sequence, and then airing for later use;
(4) accelerating germination: building a germination accelerating pool indoors, horizontally placing the cultured seedling root sections in the germination accelerating pool layer by layer, keeping the temperature in the germination accelerating pool at 20-25 ℃, and germinating for 15-20 days to form germination accelerating root sections;
(5) and (3) accelerating germination and treating root segments: soaking the seeds in a second bactericide, then soaking the seeds in rooting powder, and airing the seeds for later use;
(6) seedling culture: filling a culture medium into a seedling raising tray, correspondingly embedding the germination accelerating root section in the step (5) into the seedling raising tray, covering the culture medium, and placing the culture medium into cultivated land;
(7) building a sunshade net: building a sunshade net above the cultivated land;
(8) managing after seedling culture: watering the culture medium regularly, spraying insecticide regularly after seedling emergence, and transplanting after the seedling is cultivated until the seed-setting stichopus japonicus seedlings are grown;
in the step (3), the first bactericide is soaked for 2-3 minutes and then soaked for 1.5-2.5 hours by using the cytokinin, wherein the first bactericide and the cytokinin are carbendazim solution and 2 mg/L6-BA respectively; in the step (5), the second bactericide is soaked for 2-3 minutes and then the rooting powder is soaked for 0.8-1.2 hours, and the second bactericide and the rooting powder are respectively carbendazim solution and ABT rooting powder of 900-;
the specific steps in the step (4) are as follows:
1) building a rectangular germination accelerating pond indoors;
2) sun-dried straws with the thickness of 8-12cm are paved at the bottom and the periphery of the germination accelerating pond; then, rice bran is paved, and the thickness of the rice bran is 4-6 cm; laying 3-4 layers of straw paper;
3) laying the cultured seedling root sections above the straw paper layer by layer, wherein the stacking height of the cultured seedling root sections is 4-6cm, 8-12cm thick straw is laid above the stacked seedling root sections, and cotton quilts or cotton blankets are covered above the straw for heat preservation;
4) and keeping the temperature in the germination accelerating pool at 20-25 ℃, and forming a germination accelerating root section after the germination of the sprouts for 15-20 days.
2. The method for rapidly cultivating a panax japonicus plantlet according to claim 1, wherein in the step (1), the seed selection time is from 9 months to 10 months per year.
3. The rapid breeding method of a Stichopus japonicus selenka as claimed in claim 1, wherein in step (1), the growth time of the Stichopus japonicus selenka seedling is more than 4 years and the diameter of the enlarged node is not less than 0.3 cm.
4. The method for rapidly cultivating a holothuria macrocephala koidz seedling as claimed in claim 1, wherein in the step (2), the root section of the cultivated holothuria koidz seedling comprises an expansion joint, and the length of the bridge from the expansion joint to the end part is 1-2 cm.
5. The method for rapidly cultivating a holothuria macrocephala koidz seedling as claimed in claim 1, wherein in the step (6), the culture medium is forest humus soil, and the pretreatment method of the culture medium is as follows:
a) spraying the culture medium with 900-fold carbendazim diluent of 1100 times to make the water content reach 60-70%;
b) the film is used after being covered for 3-4 days for disinfection and sterilization, and the water content of the culture medium is kept at 55-65%.
6. The rapid cultivation method of large seedlings of panax japonicus as claimed in claim 1, wherein in the step (6), the seedling raising tray is provided with water leakage holes, the caliber of the seedling raising tray is greater than or equal to 6cm, and the hole depth is greater than or equal to 5 cm.
7. The method for rapidly cultivating the large seedlings of the panax japonicus as claimed in claim 1, wherein in the step (8), the watering period of the culture medium is 1-3 days, and the water content of the culture medium is kept to be 55-65%; the pesticide is prepared by 800 times of amobam liquid and 1000 times of dimethoate, and the spraying period of the pesticide is 10-15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811327117.5A CN109463233B (en) | 2018-11-08 | 2018-11-08 | Method for quickly cultivating large seedlings of panax japonicus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811327117.5A CN109463233B (en) | 2018-11-08 | 2018-11-08 | Method for quickly cultivating large seedlings of panax japonicus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109463233A CN109463233A (en) | 2019-03-15 |
| CN109463233B true CN109463233B (en) | 2021-12-28 |
Family
ID=65672365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811327117.5A Active CN109463233B (en) | 2018-11-08 | 2018-11-08 | Method for quickly cultivating large seedlings of panax japonicus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109463233B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255255A (en) * | 2014-09-23 | 2015-01-07 | 维西傈山中药材经营有限公司 | Method for breeding rhizoma panacis majoris seedlings in high and cold areas |
| CN107873449A (en) * | 2017-11-17 | 2018-04-06 | 重庆市中药研究院 | A kind of breeding method of polygerm panax japonicus seedling |
-
2018
- 2018-11-08 CN CN201811327117.5A patent/CN109463233B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255255A (en) * | 2014-09-23 | 2015-01-07 | 维西傈山中药材经营有限公司 | Method for breeding rhizoma panacis majoris seedlings in high and cold areas |
| CN107873449A (en) * | 2017-11-17 | 2018-04-06 | 重庆市中药研究院 | A kind of breeding method of polygerm panax japonicus seedling |
Non-Patent Citations (1)
| Title |
|---|
| 温度对珠子参种胚生长发育的影响;赵新礼等;《安徽农业科学》;20160923;第44卷(第24期);第125-126页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109463233A (en) | 2019-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103081683B (en) | Improved American red maple twig cutting propagation method | |
| CN101803521B (en) | Greenhouse seedling raising method of Psammosilene tunicoides seed | |
| CN103548513B (en) | A kind of tuniclike psammosilene root open-air rearing method | |
| CN102349412A (en) | Cultivation method of scutellaria | |
| CN105393742A (en) | Open field ginger large-area seed propagation cultivation method | |
| CN104255255B (en) | A kind of method of extremely frigid zones Panax Japonicus Var. Major nursery | |
| CN103858724A (en) | Breeding method for high-quality walnut seedlings | |
| CN102612981A (en) | Quick cultivation technique for Paris polyphylla | |
| CN104186175A (en) | Underwood planting method of anoectochilus roxburghii | |
| CN106900316B (en) | Method for natural propagation of wild large-flower flax grass seeds | |
| CN105918051A (en) | Greenhouse cultivation method for nectarines | |
| CN110771462B (en) | Method for planting paddy and dry land dual-purpose dry rice | |
| CN1180673C (en) | Method for interplanting finger citron, balsom pear and notoginseng in northern region | |
| CN104798568B (en) | Interplanting method for corn and paris polyphylla | |
| CN106922348A (en) | A kind of implantation methods of high yield purple potato | |
| CN104885721A (en) | Method for interplanting arctium lappa and atractylodes macrocephala | |
| CN105993629A (en) | Grafting method for increasing eggplant yield | |
| CN106305254A (en) | High-yield cultivation method for early-fruit thin-shell walnuts | |
| CN101658098A (en) | Method for cutting and propagating creeping stems of primula pseudodenticulata pax view | |
| CN110771463A (en) | Ratooning rice cultivation method and ratooning rice seeds | |
| CN109463233B (en) | Method for quickly cultivating large seedlings of panax japonicus | |
| CN113647299B (en) | Polygonatum kingianum planting method in under-forest herb co-cultivation mode | |
| CN112470830B (en) | Seed propagation technology of rhizoma atractylodis in asteraceae | |
| CN107517715A (en) | A kind of implantation methods of the stem of noble dendrobium | |
| CN100364378C (en) | Cultivation technique of ixeris sonchifolia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |