CN109406799B

CN109406799B - Sample analysis apparatus, reagent adding device, and control method thereof

Info

Publication number: CN109406799B
Application number: CN201710703970.1A
Authority: CN
Inventors: 章姚辉; 张明伟
Original assignee: Beijing Precil Instrument Co Ltd
Current assignee: Beijing Mindray Medical Instrument Co ltd
Priority date: 2017-08-16
Filing date: 2017-08-16
Publication date: 2022-03-04
Anticipated expiration: 2037-08-16
Also published as: CN114518466A; CN109406799A

Abstract

The invention provides a reagent adding device, which comprises: the driving mechanism is used for outputting rotary motion and reciprocating lifting motion; the fixing frame is connected to the driving mechanism, the driving mechanism drives the fixing frame to do reciprocating lifting motion and rotating motion, and the fixing frame comprises a first fixing end and a second fixing end; the reagent needle mechanism comprises a first reagent needle and a second reagent needle, the first reagent needle is arranged on the first fixing end, and the second reagent needle is arranged on the second fixing end; a heating mechanism for heating at least one of the first and second reagent needles; the supporting frame is connected with and supports the fixing frame; the utilization rate of the driving mechanism is improved, and the efficiency of the sample analysis equipment is improved. The invention also provides a sample analysis device and a control method of the reagent adding device.

Description

Sample analysis apparatus, reagent adding device, and control method thereof

Technical Field

The invention relates to the technical field of medical equipment, in particular to sample analysis equipment, a reagent adding device and a control method thereof.

Background

The full-automatic coagulation analyzer is used for analyzing coagulation and anticoagulation, fibrinolysis and anti-fibrinolysis functions of blood. Fully automatic coagulation analyzers generally comprise: sample storage device, reagent cold storage plant, testing arrangement, application of sample device, grab a cup device and advance a cup device automatically. The work flow is basically as follows: the automatic cup feeding device conveys the test cup to a cup grabbing position, a cup grabbing hand in the cup grabbing device conveys the blood coagulation test cup to the test position of the test device from the cup grabbing position, a sample adding needle in the sample adding device conveys a blood sample in the sample storage device and a reaction reagent in the reagent refrigerating device to the test cup in the test position, the sample and the reagent react in the test cup, and the test device measures a reaction result.

Currently, in the field of automatic detection of medical instruments, reagent needles are generally provided for transferring reagents from a reagent refrigeration device to a test site of a testing device. Generally, the reagent needle and the driving assembly thereof are horizontally driven in the X/Y direction, and the reagent needle has a rotary swing arm structure driving the reagent needle to rotate, so as to realize reagent needle transfer. Set up a reagent needle at the rotation type rocking arm is structural, this reagent needle need wait for several seconds and heat the reagent in the reagent needle when carrying out the heating operation, just can carry out other operations such as adding reagent after the heating is accomplished, can not carry out other operations when carrying out the heating operation promptly, actuating mechanism can be in quiescent condition and waits that the reagent needle heating finishes to drive the reagent needle rotation again, can lead to the wasting of resources like this, make the drive assembly utilization ratio not high, and then influence the operating efficiency of full-automatic blood coagulation analysis appearance, can not make full-automatic blood coagulation analysis appearance realize better function.

Disclosure of Invention

In view of the above, it is necessary to provide a reagent adding device capable of improving the utilization rate and thus the operation efficiency, a control method applied to the reagent adding device, and a sample analysis apparatus including the reagent adding device, in order to solve the problem that the utilization rate of a driving assembly is not high due to the fact that a reagent is transferred through one reagent needle at present.

The above purpose is realized by the following technical scheme:

a reagent adding device comprising:

the driving mechanism is used for outputting rotary motion and reciprocating lifting motion;

the fixing frame is connected to the driving mechanism, the driving mechanism drives the fixing frame to do reciprocating lifting motion and rotating motion, and the fixing frame comprises a first fixing end and a second fixing end;

the reagent needle mechanism comprises a first reagent needle and a second reagent needle, the first reagent needle is arranged on the first fixing end, and the second reagent needle is arranged on the second fixing end;

a heating mechanism for heating at least one of the first and second reagent needles; and

and the supporting frame is connected with and supports the fixing frame.

In one embodiment, an included angle between a connecting line between the first fixing end and the center of the fixing frame and a connecting line between the second fixing end and the center of the fixing frame ranges from 30 degrees to 180 degrees.

In one embodiment, an included angle between a line connecting the first fixed end and the center of the fixing frame and a line connecting the second fixed end and the center of the fixing frame is 180 °.

In one embodiment, the heating mechanism comprises a first heating part and a second heating part, wherein the first heating part is used for heating the reagent in the first reagent needle; the second heating component is used for heating the reagent in the second reagent needle.

In one embodiment, the reagent adding device further comprises a control mechanism, the control mechanism comprises a first temperature control main board and a second temperature control main board, and the first temperature control main board and the second temperature control main board are respectively arranged on the fixing frame;

the first temperature control main board is electrically connected with the first heating part and is used for controlling the heating temperature of the first heating part to the reagent; the second temperature control main board is electrically connected with the second heating part and used for controlling the heating temperature of the second heating part to the reagent.

In one embodiment, the control mechanism further includes a first detection main board and a second detection main board, and the first detection main board and the second detection main board are both disposed on the fixing frame;

the first detection main board is used for detecting the liquid level height in a reagent bottle for sucking a reagent by the first reagent needle; the second detection main board is used for detecting the liquid level height in a reagent bottle for sucking the reagent by the second reagent needle.

In one embodiment, the reagent adding device further comprises a cleaning mechanism, wherein the cleaning mechanism comprises a main cleaning pipe, a first cleaning branch pipe and a second cleaning branch pipe;

one end of the first cleaning branch pipe is communicated with the first reagent needle, and the other end of the first cleaning branch pipe is communicated with the cleaning main pipe; one end of the second cleaning branch pipe is communicated with the second reagent needle, and the other end of the second cleaning branch pipe is communicated with the cleaning main pipe.

In one embodiment, a main cleaning valve is arranged on the main cleaning pipe, a first branch cleaning valve is arranged on the first branch cleaning pipe, and a second branch cleaning valve is arranged on the second branch cleaning pipe; wherein the content of the first and second substances,

the main cleaning valve and the first sub cleaning valve are opened, and the second sub cleaning valve is closed to control the cleaning of the first reagent needle;

the first cleaning branch valve is opened, and the cleaning main valve and the second cleaning branch valve are closed so as to control the first reagent needle to suck or spit out a reagent.

In one embodiment, the main wash valve and the first branch wash valve are both closed to control heating of reagent in the first reagent needle.

In one embodiment, the driving mechanism includes a rotating shaft, a first driving assembly and a second driving assembly, the fixing frame is disposed on the rotating shaft, the first driving assembly is mounted on the second driving assembly, the first driving assembly and the second driving assembly are both connected to the rotating shaft, the first driving assembly is used for driving the rotating shaft to rotate, and the second driving assembly is used for driving the rotating shaft to lift.

Also relates to a control method of the reagent adding device, which comprises the following steps:

the fixed frame of the reagent adding device drives a first reagent needle and a second reagent needle to do lifting motion and rotating motion, and the first reagent needle and the second reagent needle perform operations of cleaning, reagent sucking, reagent heating and reagent spitting;

when the reagent in the first reagent needle is heated, the second reagent needle rotates to a corresponding position to perform one or more combined operations of cleaning, sucking the reagent, heating the reagent and spitting the reagent.

In one embodiment, when the second reagent needle performs a plurality of combined operations, the second reagent needle is cyclically performed in an order of arrangement of washing, aspirating a reagent, heating a reagent, and spitting a reagent.

In one embodiment, when the reagent in the second reagent needle is heated, the first reagent needle rotates to the corresponding position to sequentially perform one or more combined operations of reagent spitting, washing, reagent sucking and reagent heating.

In one embodiment, when the first reagent needle performs a cleaning operation, the cleaning solution path of the first reagent needle is switched on, and after the cleaning operation is completed, the cleaning solution path is switched off;

when the first reagent needle performs reagent sucking operation, the liquid sucking and spitting path of the first reagent needle is switched on, and after the reagent is sucked, the liquid sucking and spitting path is switched off;

when the first reagent needle executes the operation of discharging the reagent, the liquid sucking and discharging path of the first reagent needle is connected, and after the reagent is discharged, the liquid sucking and discharging path is disconnected.

The sample analysis device comprises a sample incubation device, a reagent storage device, a sample detection device and a reagent adding device according to any one of the technical characteristics;

the sample incubation device and the reagent storage device are arranged side by side, the sample detection device is arranged on one side of the sample incubation device and the reagent storage device, the reagent adding device is arranged among the sample incubation device, the reagent storage device and the sample detection device, and the reagent adding device can transfer the reagent in the reagent storage device to the sample incubation device or the sample detection device.

In one embodiment, when a first reagent needle of the reagent adding device sucks a reagent in the reagent storage device, a space where a second reagent needle is located is a first avoiding space;

when the first reagent needle of the reagent adding device discharges a reagent on the sample incubation device, the space where the second reagent needle is located is a second avoiding space;

when the first reagent needle of the reagent adding device discharges a reagent, the space where the second reagent needle is located is a third avoiding space;

when the first reagent needle of the reagent adding device is cleaned, the space where the second reagent needle is located is a fourth avoiding space.

In one embodiment, the sample analysis apparatus further comprises a waterproof member, the sample detection device comprises a magnetic bead method detection mechanism, and the waterproof member is covered between the magnetic bead method detection mechanism and the reagent storage device.

The invention has the beneficial effects that:

according to the sample analysis equipment, the reagent adding device and the control method thereof, the first reagent needle and the second reagent needle are respectively arranged on the first fixed end and the second fixed end of the fixing frame, so that the driving mechanism can drive the first reagent needle and the second reagent needle to do lifting motion, and the first reagent needle and the second reagent needle can rotate to a position for absorbing a reagent or spitting the reagent; the driving mechanism drives the first reagent needle and the second reagent needle to do lifting movement, so that the first reagent needle and the second reagent needle can absorb or discharge the reagent; moreover, when the first reagent needle sucks or discharges the reagent, the second reagent needle can heat the reagent stored in the first reagent needle, so that the heated second reagent needle can transfer the reagent, and meanwhile, the first reagent needle which is not heated is heated; the problem that the utilization rate of a driving assembly is not high due to the fact that a reagent needle transfers the reagent at present is effectively solved, when a first reagent needle absorbs or spits out the reagent, a second reagent heats the reagent in the first reagent needle, the utilization rate of a driving mechanism is improved, the reagent transfer efficiency is further improved, the sample analysis equipment efficiency is improved, and the sample analysis equipment can exert a better effect.

Drawings

FIG. 1 is a perspective view of a reagent adding apparatus according to one embodiment of the present invention;

FIG. 2 is a top view of the reagent loading device of FIG. 1 in a sample analysis apparatus;

FIG. 3 is a fluid path diagram of a first reagent needle and a second reagent needle in the reagent adding device according to the embodiment of the present invention;

FIG. 4 is a flowchart illustrating the operation of the reagent adding apparatus 100 according to an embodiment of the present invention;

FIG. 5 is a flow chart of the operation of the first and second reagent needles shown in FIG. 4;

FIG. 6 is a flow chart of the operation of the first reagent needle;

wherein:

100-a reagent adding device;

110-a drive mechanism;

111-a rotating shaft;

112-a first drive assembly; 1121 — a first drive motor; 1122-first transmission member;

113-a second drive assembly; 1131 — a second drive motor; 1132 — a second transmission member;

120-a fixed mount;

121-a first rocker arm;

122-a second rocker arm;

130-a reagent needle mechanism;

131-a first reagent needle;

132-a second reagent needle;

140-a support frame;

150-a control mechanism;

151-a first temperature control main board;

152-a second temperature control main board;

153-a first detection motherboard;

154-a second detection motherboard;

160-a cleaning mechanism;

161-cleaning the main pipe;

162-first cleaning manifold;

163-second wash manifold;

164-cleaning the main valve;

165-a first purge branch valve;

166-a second purge branch valve;

167-a pump;

200-a reagent storage device;

300-a sample incubation device;

400-sample detection device.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the sample analyzer, the reagent adding device and the control method thereof according to the present invention are further described in detail by the following embodiments with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings). In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1 and 2, fig. 1 is a perspective view of a reagent loading device 100 according to an embodiment of the present invention, and fig. 2 is a top view of the reagent loading device 100 shown in fig. 1 in a sample analysis apparatus. The invention provides a reagent adding device 100, which is applied to a sample analysis device, and is used for transferring a reagent from a reagent storage device 200 to a sample incubation device 300 of the sample analysis device or transferring the reagent to a sample detection device 400 of the sample analysis device, so that the sample and the reagent are mixed and then the sample is detected. After the sample analysis equipment adopts the reagent adding device 100, the utilization rate of the driving mechanism 110 can be improved, so that the reagent transfer efficiency is improved, the efficiency of the sample analysis equipment is improved, and the sample analysis equipment can exert better effect.

In the present invention, the reagent adding apparatus 100 includes a driving mechanism 110, a holder 120, a reagent needle mechanism 130, a heating mechanism (not shown), and a holder 140. The supporting frame 140 can play a role in supporting and fixing, the driving mechanism 110 is arranged on the supporting frame 140, and the driving mechanism 110 is connected and fixed through the supporting frame 140, so that the driving mechanism 110 is reliably installed and fixed, and the driving mechanism 110 is ensured to move accurately and reliably. The driving mechanism 110 is used for outputting rotary motion and reciprocating lifting motion, the driving mechanism 110 is connected with the fixing frame 120, and the reagent needle mechanism 130 is installed on the fixing frame 120. Thus, the driving mechanism 110 can drive the fixing frame 120 to perform lifting motion and rotational motion, so that the fixing frame 120 drives the reagent needle mechanism 130 to perform lifting motion and rotational motion. The reagent needle mechanism 130 is used to transfer reagents into the sample incubation device 300 or the sample testing device 400. The fixing frame 120 drives the reagent needle mechanism 130 to perform lifting movement, and the reagent needle mechanism 130 can perform operations of sucking or discharging a reagent; when the fixing frame 120 drives the reagent needle mechanism 130 to rotate, the reagent needle mechanism 130 can rotate to a corresponding position, such as a reagent sucking station of the reagent storage device 200, a first reagent adding station of the sample incubation device 300, or a second reagent adding station of the sample detection device 400, so that the reagent needle mechanism 130 can conveniently suck or spit a reagent.

Specifically, the fixing frame 120 includes a first fixing end and a second fixing end. The reagent needle mechanism 130 includes a first reagent needle 131 and a second reagent needle 132, the first reagent needle 131 being disposed on the first fixed end, and the second reagent needle 132 being disposed on the second fixed end. Optionally, the fixing frame 120 includes a first swing arm 121 and a second swing arm 122, one end of the first swing arm 121 is fixed to the driving mechanism 110, the other end of the first swing arm 121 extends in a direction away from the driving mechanism 110, one end of the second swing arm 122 is also fixed to the driving mechanism 110, and the other end of the second swing arm 122 also extends in a direction away from the driving mechanism 110. The first fixed end is located on an end of the first swing arm 121 away from the driving mechanism 110, and the second fixed end is located on an end of the second swing arm 122 away from the driving mechanism 110. The first reagent needle 131 is fixed to a first fixed end of the first swing arm 121, and the second reagent needle 132 is fixed to a second fixed end of the second swing arm 122. Moreover, the end of the first swing arm 121 connected to the driving mechanism 110 and the end of the second swing arm 122 connected to the driving mechanism 110 coincide, that is, the first swing arm 121 and the second swing arm 122 are disposed in a coplanar manner, so that the first reagent needle 131 and the second reagent needle 132 can be at the same height, and the operations of sucking and discharging the reagent can be conveniently performed. The driving mechanism 110 can drive the first swing arm 121 and the second swing arm 122 to perform a rotation motion and a lifting motion, so that the first swing arm 121 drives the first reagent needle 131 to move, and the second swing arm 122 drives the reagent needle to move, so that the first reagent needle 131 and the second reagent needle 132 can move synchronously.

Specifically, the heating mechanism is provided in the first reagent needle 131 and/or the second reagent needle 132, that is, at least one of the first reagent needle 131 and the second reagent needle 132 is provided with the heating mechanism. The heating mechanism can heat the reagent in at least one of the first reagent needle 131 and the second reagent needle 132. The heating mechanism can heat the reagent in the first reagent needle 131 alone, the reagent in the second reagent needle 132 alone, or the reagents in the first and second reagent needles 131 and 132. Preferably, the first reagent needle 131 and the second reagent needle 132 have a heating function, that is, the heating mechanism includes a first heating member and a second heating member, the first heating member is disposed in the first reagent needle 131 and is used for heating the reagent in the first reagent needle 131; the second heating member is disposed in the second reagent needle 132, and heats the reagent in the second reagent needle 132. After the first reagent needle 131 and the second reagent needle 132 suck the reagent in the reagent storage mechanism, the reagent is stored in the first reagent needle 131 and the second reagent needle 132, the first reagent needle 131 heats the reagent by a first heating part therein, the second reagent needle 132 heats the reagent by a second heating part therein, and after the temperature of the reagent reaches a preset temperature, the reagent is added into a reaction cup of the sample incubation device 300 or a reaction cup of the sample detection device 400, so that the reaction speed of the sample and the reagent is increased, and the analysis efficiency of the sample analysis device is improved. In addition, the first heating member and the second heating member can also rapidly and accurately heat the reagents in the first reagent needle 131 and the second reagent needle 132, and maintain the reagents at a preset temperature, so as to ensure the accuracy and the high efficiency of the test result.

Preferably, after the first reagent needle 131 finishes sucking the reagent, at this time, the driving mechanism 110 drives the fixing frame 120 to rotate, and the first reagent needle 131 can heat the reagent therein at any position. Meanwhile, the second reagent needle 132 may be rotated to a reagent sucking station of the reagent storage device 200 to suck a reagent, rotated to a first reagent adding station of the sample incubation device 300 to add a reagent, rotated to a second reagent adding station of the sample testing device 400 to discharge a reagent, or may heat a reagent in the second reagent needle 132, under the driving of the driving mechanism 110. That is, when the first reagent needle 131 performs the heating operation, the second reagent needle 132 may perform one or more of operations of sucking a reagent, heating the reagent, and discharging the reagent. Similarly, the first reagent needle 131 may perform the above-mentioned operation when the second reagent needle 132 heats the reagent therein. That is, when the second reagent needle 132 performs the heating operation, the first reagent needle 131 may perform one or more of operations of sucking a reagent, heating the reagent, and discharging the reagent.

The reagent adding device 100 of the present invention employs the first reagent needle 131 and the second reagent needle 132 to transfer the reagent, so that the first reagent needle 131 performs the heating operation, and the second reagent needle 132 performs the operations of reagent suction, reagent heating, and reagent discharge, so that when one of the reagent needles is heated, the other reagent needle can perform one or more of the operations of reagent suction, reagent heating, and reagent discharge, thereby improving the utilization rate of the driving mechanism 110, further improving the reagent transfer efficiency, improving the efficiency of the sample analysis equipment, and enabling the sample analysis equipment to exert a better effect.

Preferably, an included angle between a line connecting the first fixing end and the center of the fixing frame 120 and a line connecting the second fixing end and the center of the fixing frame 120 ranges from 30 ° to 180 °. That is, the angle between the first rocker arm 121 and the second rocker arm 122 is 30 ° to 180 °. The center of the holder 120 is indicated by the arrow shown in fig. 1. Therefore, when the first reagent needle 131 performs a reagent sucking or discharging operation, the second reagent needle 132 may deviate from the reagent sucking station, the first reagent adding station or the second reagent adding station, so as to prevent the second reagent needle 132 from contacting the reagent storage device 200, the sample incubation device 300 and the sample detection device 400, improve the operation reliability of the reagent adding device 100, and further ensure the usability of the sample analysis equipment. Moreover, when the first reagent needle 131 performs operations of sucking, heating and discharging a reagent, the second reagent needle 132 can heat the reagent at the other end, so that the other reagent needle can continue to work when one reagent needle performs the heating operation, the resource utilization rate is greatly improved, and the work efficiency of the sample analysis equipment is improved.

In this embodiment, the line between the first fixed end and the center of the fixing frame 120 and the point on the center line of the second fixed end and the fixing frame 120 are collinear. That is, the first swing arm 121 and the second swing arm 122 are arranged in a collinear manner on a horizontal plane, and the first fixed end and the second fixed end are respectively located at the farthest ends of the first swing arm 121 and the second swing arm 122 after being in the collinear manner, that is, the distance between the first reagent needle 131 and the second reagent needle 132 is the length of the first swing arm 121 and the second swing arm 122. Thus, the space where the second reagent needle 132 is located when the first reagent needle 131 performs operations of sucking and discharging a reagent is completely consistent with the space where the first reagent needle 131 is located when the second reagent needle 132 performs operations of sucking and discharging a reagent, which facilitates the design of the space for avoiding positions, optimizes the overall structure of the sample analysis apparatus, enables the positions of the first reagent needle 131 and the second reagent needle 132 to be more controllable, and facilitates the control of the reagent adding device 100.

As an implementation manner, the driving mechanism 110 includes a rotating shaft 111, a first driving assembly 112 and a second driving assembly 113, the fixing frame 120 is disposed on the rotating shaft 111, the first driving assembly 112 is mounted on the second driving assembly 113, both the first driving assembly 112 and the second driving assembly 113 are connected to the rotating shaft 111, the first driving assembly 112 is used for driving the rotating shaft 111 to rotate, and the second driving assembly 113 is used for driving the rotating shaft 111 to move up and down. The rotating shaft 111 is connected to the fixing frame 120, that is, the rotating shaft 111 is provided with a first swing arm 121 and a second swing arm 122, respectively, the first driving assembly 112 drives the rotating shaft 111 to rotate, and then the rotating shaft 111 drives the first swing arm 121 and the second swing arm 122 to rotate, so that the first reagent needle 131 and the second reagent needle 132 rotate to corresponding positions of the sample analysis device to perform corresponding operations. The second driving assembly 113 drives the rotating shaft 111 to perform lifting motion, and then the rotating shaft 111 drives the first rocker arm 121 and the second rocker arm 122 to perform lifting motion; when the second driving assembly 113 drives the rotating shaft 111 to drive the first reagent needle 131 and the second reagent needle 132 to move downwards, the first reagent needle 131 and the second reagent needle 132 can perform reagent sucking or reagent discharging operation, and after the reagent sucking or reagent discharging operation is completed, the second driving assembly 113 drives the rotating shaft 111 to drive the first reagent needle 131 and the second reagent needle 132 to move upwards; subsequently, the first driving assembly 112 drives the rotating shaft 111 to rotate the first reagent needle 131 and the second reagent needle 132 to corresponding positions to perform corresponding operations.

Of course, in another embodiment of the present invention, the driving mechanism 110 may separately drive the first reagent needle 131 and the second reagent needle 132 to rotate. That is, when the driving mechanism 110 performs one or more of operations of sucking a reagent, heating a reagent, and discharging a reagent while driving the first reagent needle 131 to rotate, the second reagent needle 132 is in a stationary state and heats a reagent; in contrast, when the driving mechanism 110 performs one or more of operations of sucking a reagent, heating a reagent, and discharging a reagent while driving the second reagent needle 132 to rotate, the first reagent needle 131 is in a stationary state and heats a reagent.

Specifically, the first driving assembly 112 includes a first driving motor 1121 and a first transmission member 1122. The first driving motor 1121 is mounted on the supporting frame 140, the first transmission member 1122 is mounted on the first driving motor 1121 and the rotating shaft 111, the first transmission member 1122 is connected to the output shaft of the first driving motor 1121 and the rotating shaft 111 in a transmission manner to drive the rotating shaft 111 to rotate, and then the rotating shaft 111 drives the first reagent needle 131 and the second reagent needle 132 on the fixing frame 120 to rotate. The second driving assembly 113 includes a second driving motor 1131 and a second transmission member 1132. The second driving motor 1131 is installed on the supporting frame 140, the second transmission member 1132 is installed on the second driving motor 1131 and the rotating shaft 111, and the second transmission member 1132 is connected to the output shaft of the second driving motor 1131 and the rotating shaft 111 in a transmission manner to drive the rotating shaft 111 to perform an ascending and descending motion, so that the rotating shaft 111 drives the first reagent needle 131 and the second reagent needle 132 on the fixing frame 120 to perform an ascending and descending motion. Alternatively, the first transmission member 1122 and the second transmission member 1132 may be in a synchronous belt transmission structure, and of course, may also be in a chain transmission structure or the like to transmit motion.

The specific process of transferring the reagent by the first reagent needle 131 is described in detail below: when the first reagent needle 131 transfers a reagent, the first driving assembly 112 drives the rotating shaft 111 to drive the first rocker arm 121 and the second rocker arm 122 to rotate, so that the first rocker arm 121 drives the first reagent needle 131 to rotate to the reagent sucking station of the reagent storage device 200; subsequently, the second driving assembly 113 drives the rotating shaft 111 to drive the first swing arm 121 to descend, and then the first swing arm 121 drives the first reagent needle 131 to descend, at this time, the first reagent needle 131 sucks the reagent at the reagent sucking station; after the reagent is sucked, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to ascend, and then the first rocker arm 121 drives the first reagent needle 131 to ascend; subsequently, the first reagent needle 131 heats the reagent therein by the first heating component, and when the temperature in the first reagent needle 131 reaches a preset temperature, the first reagent needle 131 can perform a reagent adding operation, at this time, the first driving assembly 112 drives the rotating shaft 111 to drive the first swing arm 121 to rotate, and then the first swing arm 121 drives the first reagent needle 131 to rotate to the first reagent adding station of the sample incubating device 300 or to the third reagent adding station of the magnetic bead method detecting mechanism. After the first reagent needle 131 rotates to the first reagent adding station of the sample incubation device 300, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to descend, and then the first rocker arm 121 drives the first reagent needle 131 to descend, and the first reagent needle 131 spits out a reagent into a reaction cup of the sample incubation module at the first reagent adding station; after the reagent is added, the second driving assembly 113 drives the rotating shaft 111 to drive the first swing arm 121 to ascend, and then the first swing arm 121 drives the reagent needle to ascend. When the first reagent needle 131 rotates to the third reagent adding station of the magnetic bead method detection mechanism; the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to descend, so that the first rocker arm 121 drives the first reagent needle 131 to descend, and the first reagent needle 131 spits a reagent into a reaction cup of the magnetic bead method detection mechanism at a third reagent adding station; after the reagent is added, the second driving assembly 113 drives the rotating shaft 111 to drive the first swing arm 121 to ascend, and then the first swing arm 121 drives the first reagent needle 131 to ascend.

It should be noted that the working process and the position of the second reagent needle 132 are completely the same as those of the first reagent needle 131, when the first reagent needle 131 heats the reagent therein by the first heating component, the first driving component 112 drives the rotating shaft 111 to drive the second reagent needle 132 to rotate to the reagent sucking station, the first reagent adding station or the second reagent adding station to perform the same operation as that of the first reagent needle 131, at this time, the position of the first reagent needle 131 does not need to be controlled, when the second reagent needle 132 heats the reagent therein by the second heating component, after the reagent in the first reagent needle 131 is heated, the first driving component 112 drives the first reagent needle 131 to rotate to the first reagent adding station or the second reagent adding station to perform the corresponding operation, and at this time, the position of the second reagent needle 132 does not need to be controlled. The operation is repeated in such a circulating way, so that the transfer of the reagent is realized, and the operation efficiency is improved.

In addition, the order of adding the sample and the reagent in the sample incubation device 300 is not limited, and the sample may be added to the reaction cup first, and then the reagent may be spitted out by the reagent adding device 100; of course, the reagent may be first dispensed into the cuvette and then the sample may be added. In this case, the reagent adding apparatus 100 of the present invention may transfer the reagent from the reagent storage apparatus 200 to the reaction cuvette with the sample of the sample incubation apparatus 300 by the reagent adding apparatus 100, or may add the reagent to an empty reaction cuvette of the sample incubation apparatus 300, and the reaction cuvette may be selected according to actual use requirements.

Optionally, the reagent adding apparatus 100 further includes a control mechanism 150, the control mechanism 150 includes a first temperature control main board 151 and a second temperature control main board 152, and the first temperature control main board 151 and the second temperature control main board 152 are respectively disposed on the fixing frame 120. The first temperature control main board 151 is electrically connected with the first heating part, and the first temperature control main board 151 is used for controlling the heating temperature of the first heating part to the reagent; the second temperature control main board 152 is electrically connected to the second heating member, and the first temperature control main board 152 is used for controlling the heating temperature of the second heating member to the reagent. The first temperature control main board 151 and the second temperature control main board 152 can ensure that the real reagent in the first reagent needle 131 and the second reagent needle 132 is heated and maintained at a preset temperature quickly and accurately, and ensure the accuracy and the high efficiency of the test result. Preferably, the first heating member and the second heating member may be heating wires, and of course, the first heating member and the second heating member may also be other structures capable of realizing a heating function.

Further, the control mechanism 150 further includes a first detection main board 153 and a second detection main board 154, and the first detection main board 153 and the second detection main board 154 are disposed on the fixing frame 120. The first detection main board 153 is electrically connected with the first reagent needle 131, and the first detection main board 153 is used for detecting the liquid level height in a reagent bottle for sucking the reagent by the first reagent needle 131; the second detection main board 154 is electrically connected to the second reagent needle 132, and the second detection main board 154 is configured to detect a liquid level height in a reagent bottle in which the second reagent needle 132 sucks a reagent, so that the control mechanism 150 can control a distance that the first reagent needle 131 and the second reagent needle 132 move downward, that is, a depth at which the first reagent needle 131 and the second reagent needle 132 are inserted downward, according to the height of the liquid level in the reagent bottle; simultaneously, when the liquid level height in the reagent bottle was crossed lowly, can also the suggestion in time change the reagent bottle or add reagent to the reagent bottle, guarantee equipment normal operating. Optionally, a first liquid level detection component is disposed in the first reagent needle 131, the first liquid level detection component is electrically connected to the first detection main board 153, and the liquid level of the end of the first reagent needle 131 is determined by the first liquid level detection component; the second reagent needle 132 is provided with a second liquid level detection component, the second liquid level detection component is electrically connected with the second detection main board 154, and the liquid level of the end of the second reagent needle 132 is determined by the second liquid level detection component. Preferably, the first liquid level detection component and the second liquid level detection component may be sensors, and of course, may also be other structures capable of realizing liquid level detection.

Moreover, first temperature control mainboard 151 and first detection mainboard 153 set up relatively on first rocking arm 121 of mount 120, have the space between first temperature control mainboard 151 and the first detection mainboard 153, make things convenient for the line of walking of other pipelines, electric wires etc. of first reagent needle 131, save space, guarantee that the structure is neat and orderly. Second temperature control mainboard 152 and second detect mainboard 154 and set up relatively on the second rocking arm 122 of mount 120, have the space between second temperature control mainboard 152 and the second detection mainboard 154, make things convenient for the line of other pipelines, electric wires etc. of second reagent needle 132, save space, guarantee that the structure is neat and orderly.

Referring to fig. 3, fig. 3 is a fluid path diagram of the first reagent needle 131 and the second reagent needle 132 in the reagent adding apparatus 100 according to the embodiment of the present invention. Optionally, the reagent feeding device 100 further comprises a washing mechanism 160. The cleaning mechanism 160 is used to clean the first and second reagent needles 131 and 132. In order to prevent the first and second reagent needles 131 and 132 from contaminating the sample when transferring the reagent, the first and second reagent needles 131 and 132 need to be cleaned after transferring the reagent once, so that mutual infection between the sample and the reagent and mutual infection between different reagents are avoided, and the reliability of detection of the sample analysis device is ensured. Specifically, the cleaning mechanism 160 includes a main cleaning pipe 161, a first cleaning branch pipe 162 and a second cleaning branch pipe 163, and the main cleaning pipe 161, the first cleaning branch pipe 162 and the second cleaning branch pipe 163 are disposed on the fixing frame 120. One end of the first cleaning branch pipe 162 is communicated with the first reagent needle 131, and the other end of the first cleaning branch pipe 162 is communicated with the cleaning main pipe 161; one end of the second cleaning branch pipe 163 communicates with the second reagent needle 132, and the other end of the second cleaning branch pipe 163 communicates with the cleaning main pipe 161. The first cleaning branch pipe 162 is used to clean the first reagent needle 131, and the second cleaning branch pipe 163 is used to clean the second reagent needle 132. One end of the main cleaning pipe 161, which is far from the first cleaning branch pipe 162, is connected to a liquid source, in which a cleaning liquid is stored, and the main cleaning pipe 161 can convey the cleaning liquid in the liquid source to the first reagent needle 131 through the first cleaning branch pipe 162 and to the second reagent needle 132 through the second cleaning branch pipe 163. Furthermore, the first cleaning branch pipe 162 and the second cleaning branch pipe 163 share the cleaning main pipe 161, and the cleaning liquid is supplied to the first cleaning branch pipe 162 and the second cleaning branch pipe 163 through one cleaning main pipe 161, so that the cleaning liquid is delivered to the first reagent needle 131 and the second reagent needle 132, respectively, thereby achieving the purpose of cleaning the first reagent needle 131 and the second reagent needle 132, and simultaneously reducing the number of pipelines, and further reducing the complexity of the structure. Also, the first cleaning branch pipe 162 is disposed in a space between the first temperature control main plate 151 and the first detection main plate 153, the second cleaning branch pipe 163 is disposed in a space between the second temperature control main plate 152 and the second detection main plate 154, and the cleaning main pipe 161 is disposed in the rotation shaft 111.

Further, a main cleaning valve 164 is provided on the main cleaning pipe 161, a first branch cleaning valve 165 is provided on the first branch cleaning pipe 162, and the first branch cleaning valve 165 is located between the main cleaning valve 164 and the first reagent needle 131; the second cleaning branch pipe 163 is provided with a second cleaning branch valve 166, and the second cleaning branch valve 166 is located between the cleaning main valve 164 and the second reagent needle 132. The main cleaning valve 164 is used to control the opening and closing of the main cleaning pipe 161, the first branch cleaning valve 165 is used to control the opening and closing of the first branch cleaning pipe 162, and the second branch cleaning valve 166 is used to control the opening and closing of the second branch cleaning pipe 163. The main purge valve 164 and the first sub-purge valve 165 are opened, and the second sub-purge valve 166 is closed, so as to control the purging of the first reagent needle 131. When the first reagent needle 131 performs the cleaning operation, the second cleaning branch valve 166 is closed, the cleaning main valve 164 and the first cleaning branch valve 165 are opened, the cleaning solution enters the first reagent needle 131 through the cleaning main pipe 161 and the first cleaning branch pipe 162 to perform the cleaning operation, after the cleaning operation is completed, the cleaning main valve 164 is closed, and after the cleaning solution is drained, the first cleaning branch valve 165 is closed.

The cleaning mechanism 160 further includes a pump 167, and the pump 167 is disposed on the cleaning main pipe 161 and is located between the cleaning main valve 164 and the first cleaning branch valve 165 and the second cleaning branch valve 166. For example, when the first branch purge valve 165 is opened, the main purge valve 164 and the second branch purge valve 166 are closed, and the pump 167 controls the first reagent needle 131 to suck or discharge the reagent. When the first reagent needle 131 performs a reagent sucking or discharging operation, the main cleaning valve 164 and the second cleaning branch valve 166 are closed, the first cleaning branch valve 165 is opened, and a reagent is sucked or discharged by the pump 167. When the reagent is aspirated, the reagent is stored in the first reagent needle 131. Still further, the main purge valve 164 and the branch purge valve 165 are both closed to control the first reagent needle 131 to heat the reagent. When the first reagent needle 131 heats the reagent therein, the main wash valve 164 and the branch first wash valve 165 are closed, and the reagent is prevented from flowing to the main wash pipe 161 or out of the first reagent needle 131.

Referring to fig. 4 and 5, fig. 4 is a flowchart illustrating an operation of the reagent adding apparatus 100 according to an embodiment of the present invention, and fig. 5 is a flowchart illustrating operations of the first reagent needle 131 and the second reagent needle 132 illustrated in fig. 4. The present invention also provides a control method of a reagent adding apparatus 100, which is applied to the reagent adding apparatus 100 of the above embodiment, and the control method of the reagent adding apparatus 100 includes the following steps:

step S1: the fixing frame 120 of the reagent adding apparatus 100 drives the first reagent needle 131 and the second reagent needle 132 to perform a lifting motion and a rotating motion, the first reagent needle 131 performs operations of cleaning, sucking a reagent, heating the reagent, and discharging the reagent, and the second reagent needle 132 performs operations of cleaning, sucking the reagent, heating the reagent, and discharging the reagent.

Step S2: when the reagent in the first reagent needle 131 is heated, the second reagent needle 132 is rotated to a corresponding position to perform one or more combined operations of washing, aspirating the reagent, heating the reagent, and spitting the reagent.

When the reagent adding device 100 of the present invention transfers a reagent, the driving mechanism 110 of the reagent adding device 100 drives the fixing frame 120 to drive the first reagent needle 131 and the second reagent needle 132 to perform a lifting motion and a rotating motion, so that the first reagent needle 131 and the second reagent needle 132 perform operations of cleaning, sucking a reagent, heating a reagent, and discharging a reagent, and the operation process of the first reagent needle 131 and the second reagent needle 132 performing the operations has been described above, and is not repeated herein.

It can be understood that, before the reagent is sucked, a cleaning operation is usually performed on the first reagent needle 131, and after the cleaning operation is completed, the driving mechanism 110 drives the first reagent needle 131 to rotate to the reagent sucking station and suck the reagent; after the first reagent needle 131 sucks the reagent and heats the reagent therein, the driving mechanism 110 may drive the fixing frame 120 to rotate, so that the second reagent needle 132 rotates to the cleaning station to perform a cleaning operation and rotates to the reagent sucking station of the reagent storage device 200 to suck the reagent, or the second reagent needle 132 rotates to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400, or the second reagent needle 132 also performs a corresponding heating operation after sucking the reagent, although the second reagent needle 132 may also perform a combination of a plurality of operations of cleaning, sucking the reagent, heating the reagent, and discharging the reagent.

Alternatively, when the reagent in the second reagent needle 132 is heated, the first reagent needle 131 is rotated to a corresponding position to perform one or more combined operations of washing, aspirating the reagent, heating the reagent, and spitting the reagent. When the second reagent needle 132 heats the reagent therein after the reagent is sucked, the driving mechanism 110 may drive the first reagent needle 131 to rotate to the cleaning station for cleaning operation, may drive the first reagent needle 131 to rotate to the reagent sucking station of the reagent storage device 200 for sucking the reagent, may also drive the first reagent needle 131 to rotate to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400, may drive the first reagent needle 131 to perform corresponding heating operation after the reagent is sucked, and may also drive the first reagent needle 131 to perform a combination of a plurality of operations of cleaning, sucking the reagent, heating the reagent, and spitting the reagent. For example, a washing operation and a reagent sucking operation are performed; or, performing a reagent sucking operation, a reagent heating operation, a reagent spitting operation, and a washing operation; alternatively, a washing operation, a reagent sucking operation, a reagent heating operation, a reagent discharging operation, and the like are performed.

Further, when the second reagent needle 132 performs a plurality of kinds of combination operations, the second reagent needle 132 is cyclically performed in the order of arrangement of washing, aspirating a reagent, heating a reagent, and discharging a reagent.

That is, the operations performed by the second reagent needle 132 are cyclically performed in the order of washing, aspirating a reagent, heating a reagent, and discharging a reagent; further, when the first reagent needle 131 performs a plurality of kinds of combination operations, the first reagent needle 131 is also cyclically performed in the order of arrangement of the washing, the reagent sucking, the reagent heating, and the reagent discharging.

In a specific embodiment of the present invention, step S10: the driving mechanism 110 drives the first reagent needle 131 to rotate to the cleaning station and perform the cleaning operation; after the first reagent needle 131 is cleaned, step S11 is executed, in which the driving mechanism 110 drives the first reagent needle 131 to rotate to the reagent sucking station of the reagent storage device 200 to suck the reagent; after the aspiration is completed, step S12 is performed, the first reagent needle 131 heats the reagent therein, and simultaneously, step S13 is performed, the driving mechanism 110 drives the second reagent needle 132 to rotate to the cleaning station; after the second reagent needle 132 is cleaned, step S14 is executed, in which the driving mechanism 110 drives the second reagent needle 132 to rotate to the reagent sucking station of the reagent storage device 200 to suck the reagent; after the second reagent needle 132 sucks the reagent, the step S15 is executed, and the second reagent needle 132 heats the reagent therein; meanwhile, after the reagent in the first reagent needle 131 is heated to the desired temperature, step S16 is executed, and the driving mechanism 110 drives the first reagent needle 131 to rotate to the first reagent adding station of the sample incubation apparatus 300 or the second reagent adding station of the sample detection apparatus 400; after the first reagent needle 131 discharges the reagent therein, step S17 is executed, and the driving mechanism 110 drives the first reagent needle 131 to rotate to the cleaning station to execute the cleaning operation; after the washing is completed, when the temperature of the reagent in the second reagent needle 132 is heated to a desired temperature, step S18 is executed, and the driving mechanism 110 drives the second reagent needle 132 to rotate to the first reagent adding station of the sample incubation apparatus 300 or the second reagent adding station of the sample testing apparatus 400; the second reagent needle 132 discharges the reagent therein, step S19 is executed, and then the driving mechanism 110 drives the second reagent needle 132 to rotate to the cleaning station to execute the cleaning operation; when the reagent in the second reagent needle 132 needs to be heated, the driving mechanism 110 may drive the first reagent needle 131 to perform operations of reagent aspiration and reagent heating, and perform steps S10 to S12. When the reagent adding apparatus 100 repeatedly transfers the reagent, the first and second reagent needles 131 and 132 are cyclically performed in the order of step S10 to step S19, so that the first and second reagent needles 131 and 132 are alternately used. When the second reagent needle 132 heats the reagent therein, the sequence of the first reagent needle 131 is determined by the heating time of the second reagent needle 132. For example, the heating time of the second reagent needle 132 is short, the first reagent needle 131 may be able to perform only a washing operation or perform a washing and reagent aspirating operation, and the like; when the second reagent needle 132 is heated for a long time, the first reagent needle 131 may perform operations of performing washing, aspirating a reagent and heating the reagent or perform operations of performing washing, aspirating a reagent, heating the reagent and spitting the reagent.

Thus, when the first reagent needle 131 heats the reagent therein, the second reagent needle 132 can perform operations of cleaning, sucking the reagent, heating the reagent and spitting the reagent, and when the second reagent needle 132 heats the reagent therein, the first reagent needle 131 can perform operations of cleaning, sucking the reagent, heating the reagent and spitting the reagent, so that when one of the reagent needles performs operation of heating the reagent, the other reagent needle can perform other operations, that is, the other reagent needle can perform various actions, so that the first reagent needle 131 and the second reagent needle 132 are not in an idle state, the utilization rate of the driving mechanism 110 is improved, the resource utilization rate is greatly improved, and the working efficiency of the sample analysis device is further improved.

Of course, in another embodiment of the present invention, the first reagent needle 131 and the second reagent needle 132 may be sequentially performed, that is, after the first reagent needle 131 sequentially performs the operations of washing, aspirating the reagent, heating the reagent, and discharging the reagent, the second reagent needle 132 sequentially performs the operations of washing, aspirating the reagent, heating the reagent, and discharging the reagent. This also enables transfer of reagents.

Referring to fig. 3 and 6, fig. 6 is a flowchart illustrating the operation of the first reagent needle 131. As one possible embodiment, when the first reagent needle 131 performs the washing operation, the washing liquid path of the first reagent needle 131 is turned on, and after the washing is completed, the washing liquid path is turned off;

when the first reagent needle 131 performs a reagent sucking operation, the liquid sucking and discharging path of the first reagent needle 131 is connected, and after the reagent sucking operation is completed, the liquid sucking and discharging path is disconnected;

when the first reagent needle 131 performs a reagent discharge operation, the liquid suction/discharge path of the first reagent needle 131 is closed, and after the reagent discharge is completed, the liquid suction/discharge path is opened.

The liquid sucking and discharging path of the first reagent needle 131 is connected with the first cleaning branch pipe 162 of the first reagent needle 131 and the first cleaning branch valve 165 arranged thereon, and the first cleaning branch valve 165 controls the connection and disconnection of the first cleaning branch pipe 162; the cleaning liquid path includes a liquid suction and discharge path and a cleaning main pipe 161 communicating with the liquid suction and discharge path. During cleaning, the cleaning liquid path supplies the cleaning liquid to the suction and discharge liquid path through the cleaning main pipe 161, and after cleaning is completed, the cleaning main pipe 161 of the cleaning liquid path is closed to complete the cleaning operation. When the first reagent needle 131 sucks or discharges a reagent, the liquid sucking/discharging path can facilitate the first reagent needle 131 to suck or discharge the reagent. When heating, inhale and spit the reagent and be in the confined state, avoid appearing the weeping problem.

The connection and disconnection of the cleaning liquid path and the aspiration and discharge liquid path of the first reagent needle 131 will be described with reference to the first reagent needle 131 as an example. When the first reagent needle 131 performs the cleaning operation, the step S30 is performed, the first cleaning branch valve 165 is opened, the second cleaning branch valve 166 is closed, the step S31 is performed, the cleaning main valve 164 is opened, and the step S32 is performed, in which the cleaning solution is introduced into the first reagent needle 131 to perform the cleaning operation on the first reagent needle 131; after the completion of the washing, step S33 is executed to close the main washing valve 164, at which time the first branch washing valve 165 is in an open state and the second branch washing valve 166 is in a closed state, step S34 is executed to suck the reagent into the first reagent needle 131, and after the suction is completed, step S35 is executed to close the first branch washing valve 165. Subsequently, the step S36 is performed, and the first reagent needle 131 performs a heating operation; after the heating is completed, step S37 is executed, the first cleansing branch valve 165 is opened, the second cleansing branch valve 166 is still in the closed state, step S38 is executed, and the first reagent needle 131 performs the operation of ejecting the reagent. Then, the cleaning main valve 164 is opened, and a cleaning solution is introduced into the first reagent needle 131 to perform a cleaning operation on the first reagent needle 131; after the cleaning is completed, the cleaning main valve 164 is closed. This cycle realizes operations of cleaning the first reagent needle 131, sucking the reagent, heating the reagent, and discharging the reagent. When the operation of the reagent adding apparatus 100 is stopped, the main wash valve 164 is closed after the first reagent needle 131 is washed, and the first branch wash valve 165 is closed again. It should be noted that the second reagent needle 132 is controlled in the same manner as the first reagent needle 131, and therefore, the detailed description thereof is omitted.

Referring to fig. 1 and 2, the present invention further provides a sample analysis apparatus, which comprises a sample incubation device 300, a reagent storage device 200, a sample detection device 400, and the reagent adding device 100 in the above embodiments. The sample incubation device 300 and the reagent storage device 200 are arranged side by side, the sample detection device 400 is arranged at one side of the sample incubation device 300 and the reagent storage device 200, the reagent adding device 100 is arranged among the sample incubation device 300, the reagent storage device 200 and the sample detection device 400, and the reagent adding device 100 can transfer the reagent in the reagent storage device 200 to the sample incubation device 300 or the sample detection device 400. The reagent adding device 100 realizes the transfer of the reagent among the sample incubation device 300, the reagent storage device 200 and the sample detection device 400, so as to realize the detection of the sample by the sample analysis equipment. The sample analysis equipment further comprises a transfer device and a stirring device, wherein the transfer device is used for transferring the reaction cups, transferring the empty reaction cups to the sample incubation device 300, transferring the reaction cups added with the samples and the reagents to the sample detection device 400, and the stirring device is used for stirring the samples and the reagents in the reaction cups, so that the samples and the reagents are uniformly mixed, and the accurate detection result is ensured.

Further, when one of the reagent needles of the reagent adding device 100 sucks the reagent in the reagent storage device 200, the other reagent needle is located in the first space. When one of the reagent needles of the reagent adding device 100 discharges the reagent on the sample incubation device 300, the other reagent needle is located in the second space. When one of the reagent needles of the reagent adding apparatus 100 discharges the reagent in the sample detection apparatus 400, the other reagent needle is located in the third space. When one of the reagent needles of the reagent adding apparatus 100 is cleaned, the other reagent needle is located in the fourth space.

Because the reagent adding device 100 is of a double-ended needle structure, the reagent adding device 100 may interfere with components of a transfer device for transferring a reagent, a stirring device, a sample incubation device 300, a reagent storage device 200, and the like, and cause a sample analysis apparatus to stop when transferring a reagent, so that a spacing space between the first reagent needle 131 and the second reagent needle 132 needs to be limited, when the first reagent needle 131 performs an operation, the second reagent needle 132 should be located in the spacing space, and correspondingly, when the second reagent needle 132 performs a corresponding operation, the first reagent needle 131 should also be located in the spacing space, so as to ensure that the reagent adding device 100 works reliably, and further improve the working reliability of the sample analysis apparatus. Specifically, when the first reagent needle 131 sucks the reagent in the reagent taking station, the space where the second reagent needle 132 is located is the first avoiding space. When the first reagent needle 131 sucks a reagent on the reagent sucking station of the reagent storage device 200, the second reagent needle 132 has a space at a position a shown in fig. 2, and the transfer device needs to avoid the second reagent needle 132, so that collision is avoided, and interference with other parts when the second reagent needle 132 is lifted and lowered is avoided. When the first reagent needle 131 adds reagent at the first reagent adding station, the space where the second reagent needle 132 is located is the second avoiding space. When the first reagent needle 131 adds a reagent at the first reagent adding station on the sample incubation device 300, the second reagent needle 132 has a space at the position B shown in fig. 2, so as to avoid collision and avoid interference with other parts when the second reagent needle 132 is lifted. When the first reagent needle 131 adds reagent at the first reagent adding station, the space where the second reagent needle 132 is located is the third avoiding space. When the first reagent needle 131 adds a reagent at the second reagent adding station on the sample detection apparatus 400, the second reagent needle 132 has a space at the position C shown in fig. 2 to avoid collision, and meanwhile, the second reagent needle 132 is prevented from interfering with other parts when being lifted. When the first reagent needle 131 is cleaned, the space where the second reagent needle 132 is located is the fourth space. When the first reagent needle 131 is cleaned, the second reagent needle 132 has a space at a position B shown in fig. 2 to avoid collision, and meanwhile, the second reagent needle 132 is prevented from interfering with other parts when being lifted. When the second reagent needle 132 performs the above operation, the first reagent needle 131 also has the above-described space.

Optionally, the sample analysis apparatus further comprises a waterproof member (not shown), and the sample detection device 400 comprises a magnetic bead method detection mechanism, and the waterproof member is covered between the magnetic bead method detection mechanism and the reagent storage device 200. In order to prevent the reagents stained on the surfaces of the first reagent needle 131 and the second reagent needle 132 from dropping on the magnetic bead method detection mechanism, the control main board is additionally provided with a waterproof component between the magnetic bead method detection mechanism and the reagent storage device 200, and the waterproof component plays a role in water isolation to prevent the water from dropping.

The technical features of the embodiments described above can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A reagent feeding device, comprising:

the supporting frame is connected with and supports the fixing frame;

the first reagent needle may perform operations of sucking, heating, and discharging a reagent, the second reagent needle may perform operations of sucking, heating, and discharging a reagent, and when one of the first and second reagent needles performs a heating operation, the other may perform one or more of operations of sucking, heating, and discharging a reagent.

2. The reagent feeding device of claim 1, wherein an angle between a line connecting the first fixing end and the center of the fixing frame and a line connecting the second fixing end and the center of the fixing frame is in a range of 30 ° to 180 °.

3. The reagent feeding device of claim 2, wherein the angle between the line connecting the first fixed end and the center of the holder and the angle between the second fixed end and the line connecting the center of the holder are 180 °.

4. The reagent feeding apparatus according to any one of claims 1 to 3, wherein the heating mechanism includes a first heating part for heating the reagent in the first reagent needle and a second heating part; the second heating component is used for heating the reagent in the second reagent needle.

5. The reagent adding device of claim 4, further comprising a control mechanism, wherein the control mechanism comprises a first temperature control main board and a second temperature control main board, and the first temperature control main board and the second temperature control main board are respectively disposed on the fixing frame;

6. The reagent feeding device of claim 5, wherein the control mechanism further comprises a first detection main board and a second detection main board, and the first detection main board and the second detection main board are both disposed on the fixing frame;

7. The reagent adding apparatus according to any one of claims 1 to 3, further comprising a cleaning mechanism including a main cleaning pipe, a first cleaning branch pipe, and a second cleaning branch pipe;

8. The reagent feeding device of claim 7, wherein a main cleaning valve is disposed on the main cleaning pipe, a first branch cleaning valve is disposed on the first branch cleaning pipe, and a second branch cleaning valve is disposed on the second branch cleaning pipe; wherein the content of the first and second substances,

9. The reagent feeding device of claim 8, wherein the main wash valve and the branch first wash valve are both closed to control heating of the reagent in the first reagent needle.

10. The reagent feeding device of claim 1, wherein the driving mechanism comprises a rotating shaft, a first driving assembly and a second driving assembly, the fixing frame is disposed on the rotating shaft, the first driving assembly is mounted on the second driving assembly, the first driving assembly and the second driving assembly are both connected to the rotating shaft, the first driving assembly is used for driving the rotating shaft to rotate, and the second driving assembly is used for driving the rotating shaft to move up and down.

11. A control method of a reagent adding device is characterized by comprising the following steps:

a fixing frame of the reagent adding device drives a first reagent needle and a second reagent needle to do lifting motion and rotating motion, and the first reagent needle and the second reagent needle perform operations of cleaning, reagent sucking, reagent heating and reagent spitting;

12. The method of controlling a reagent adding device according to claim 11, wherein when the second reagent needle performs a plurality of kinds of combination operations, the second reagent needle is cyclically performed in an order of arrangement of washing, sucking a reagent, heating a reagent, and discharging a reagent.

13. The method of claim 11, wherein when the reagent in the second reagent needle is heated, the first reagent needle is rotated to a corresponding position to sequentially perform one or more of the combination of discharging the reagent, washing, sucking the reagent, and heating the reagent.

14. The method of controlling a reagent adding apparatus according to any one of claims 11 to 13, wherein a cleaning liquid path of the first reagent needle is closed when the first reagent needle performs a cleaning operation, and the cleaning liquid path is closed after the cleaning operation is completed;

15. A sample analysis apparatus comprising sample incubation means, reagent storage means, sample detection means and reagent addition means as claimed in any one of claims 1 to 10;

16. The sample analysis apparatus according to claim 15, wherein the space in which the second reagent needle is located when the first reagent needle of the reagent adding device sucks the reagent in the reagent storage device is a first space;

17. The sample analysis apparatus of claim 15, further comprising a waterproof member, wherein the sample detection device comprises a magnetic bead assay detection mechanism, and wherein the waterproof member is disposed between the magnetic bead assay detection mechanism and the reagent storage device.