JP2001174465A - Blood coagulation-analyzing device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood coagulation-analyzing device capable of maintaining analysis accuracy without being affected by ambient temperature. SOLUTION: In a reagent-dispensing probe 20, its tip side is connected to a nozzle part 25, its rear side is connected to a pump 38 via piping, and hot water 36 is circulated from an inlet 26 of an outer cylinder part 26 to an outlet 20b for heating at a constant temperature. When the pump 38 is driven and a reagent 37 is sucked from a reagent container, the reagent 37 is also sucked into the nozzle part 25. Then, when the reagent-dispensing probe 20 is pulled from a reagent container 17 for separation and then the pump 28 is driven for fixed time, the sucked reagent is moved upward as shown in Fig. 2 is heated while being brought closer to constant temperature water and then is discharged into a reaction container. As a result, reaction liquid in the reaction container reaches a target temperature rapidly.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood coagulation analyzer having a sample dispensing section, a reagent dispensing section, and an analyzing section for analyzing a sample in a reaction container. The present invention relates to a blood coagulation analyzer having a reagent dispensing section provided with a reagent dispensing probe for preheating a reagent while dispensing the reagent into a blood coagulation analyzer.

[0002]

2. Description of the Related Art For example, a turntable type blood coagulation analyzer has a turntable capable of intermittent rotation drive and continuous rotation drive, a sample dispensing section having a sample table,
It has a reagent dispensing section equipped with a reagent tray and an analyzing section for measuring the coagulation characteristics of a sample, and disposable type reaction vessels are arranged on the turntable, and this turntable is used as a reaction vessel according to a preset time program. An analysis is performed each time.

In this analysis, for example, when a turntable is stopped, a predetermined amount of a sample dispensing probe is supplied to a reaction container located in an analysis work area such as a sample dispensing area, a reagent dispensing area, a stirring area, and a reaction area. Performs analysis operations such as sample dispensing, reagent dispensing of a predetermined amount by a reagent dispensing probe, stirring and mixing of a reaction solution, and reaction. Next, the turntable is intermittently rotated to send the reaction vessel to the next stop position,
For the reaction vessels located in the analysis work area, analysis operations such as sample dispensing, reagent dispensing, stirring, and reaction are performed in the same manner as in the previous time, and such turntable driving and analysis operation in each analysis work area are performed. Is continuously and repeatedly analyzed. With respect to the reaction vessels that have undergone the above-described processes, the reaction characteristics of the reaction solution are continuously measured by the analysis units provided in the respective storage units.

In such a turntable type blood coagulation analyzer, a liquid dispensing operation such as sample dispensing or reagent dispensing is performed by placing a sample dispensing probe or reagent dispensing in a liquid container such as a sample container or a reagent container. A liquid dispensing probe such as an injection probe is inserted, and a predetermined amount of a liquid such as a specimen or a reagent is aspirated into the liquid dispensing probe by a negative pressure in the inserted liquid dispensing probe. The liquid dispensing probe that aspirated the liquid is moved to a liquid dispensing position such as a sample dispensing position or a reagent dispensing position on the reaction line, and is sent to a liquid dispensing position such as a sample dispensing position or a reagent dispensing position on the reaction line. Liquid dispensing, such as sample dispensing or reagent dispensing, is performed by discharging the liquid sucked and collected into the liquid dispensing probe into the reaction container that has been prepared.

[0005] In such a blood coagulation analyzer, the reagent container is stored in a low-temperature bath and kept cool so that the reagent can be used without being deteriorated for a long period of time. Then, as shown in FIG. 3, the reagent dispensing probe is kept at a temperature suitable for the reaction temperature, for example, 37 ° C. by circulating constant temperature water from the inlet 20 a to the outlet 20 b, and is sucked by the reagent dispensing probe 35. By keeping the temperature of the reagent at 37 ° C. until the reagent 37 is dispensed into the reaction container, the temperature of the discharged reagent is kept constant regardless of the outside air temperature and the reagent temperature.

[0006]

The conventional blood coagulation analyzer is constructed as described above, but the constant temperature water circulating around the reagent dispensing probe is higher than the nozzle 25 as shown in FIG. The parts are circulating. Since the nozzle portion 25 is exposed to the outside air, and the reagent 37 sucked into this portion is not heated close to the constant temperature water 36,
The temperature of the entire reagent discharged into the reaction vessel falls, for example, below a target of 37 ° C., and the degree of this temperature decrease increases as the ambient temperature of the nozzle section 25 becomes lower than 37 ° C. As a result, there is a problem that the measurement accuracy is affected. The present invention has been made in view of such circumstances, and it is an object of the present invention to provide a blood coagulation analyzer that can maintain a predetermined analysis accuracy regardless of the outside air temperature and the temperature of a cold reagent. And

[0007]

In order to achieve the above object, a blood coagulation analyzer of the present invention comprises a sample dispensing probe for aspirating and collecting a sample contained in a sample container. The reagent is dispensed to the reaction container via a sample dispensing unit for dispensing the reagent and a reagent dispensing probe that suctions and collects the reagent contained in the reagent container during cold storage and heats the reagent with constant temperature water. In the blood coagulation analyzer equipped with an analyzer for measuring the reaction characteristics of the reaction solution in the reaction container, a reagent dispensing unit for aspirating a reagent into the reagent dispensing probe,
Further, there is provided a means for sucking air and heating the sucked reagent as a whole in close proximity to the constant temperature water. The blood coagulation analyzer of the present invention is configured as described above, and the entire reagent sucked by the reagent dispensing probe is heated close to constant temperature water, so that the reaction liquid reaches the target temperature quickly. Can be.

[0008]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a blood coagulation analyzer of the present invention will be described in detail with reference to embodiments. FIG. 1 is a schematic configuration diagram of an embodiment of a blood coagulation analyzer of the present invention, and FIG. 2 is a cross-sectional view of a configuration of a reagent dispensing probe and its reagent suction state. In FIG. 1, a reaction disk 2 is
Is provided at the center, and a reaction vessel rotor 3 is provided above and outside thereof. A large number of disposable reaction vessels 4 (partially omitted) are arranged on the inner circumference of the reaction vessel rotor 3 to form a reaction line 5.

The sample dispensing section 6 is composed of a sample dispensing sampling table 8 in which a large number of sample containers 7 are arranged and a sample dispenser 9. The sample dispenser 9 includes a sample dispensing probe 10. At the sample aspirating and sampling position 13 to aspirate and collect a sample from the sample container 7 located there, and then dispensing the sample collected into the reaction container 4 at the sample dispensing position 14 of the reaction line 5 I do. A reagent dispensing unit 16 is provided below the sample dispenser 9 below the intermittent rotation direction 15 of the reaction container rotor 3. The reagent dispensing section 16 is provided with a reagent tray 18 for reagent dispensing in which a large number of reagent containers 17 are arranged and a reagent dispenser 19.

As shown in the sectional view of FIG. 2, the reagent dispenser 19 has a nozzle portion 25 at the tip, and is connected from an inlet 20a to an outlet 2a.
A reagent dispensing probe 20 provided with an outer tube portion 26 for circulating a constant temperature water 36 in the 0b direction;
Pump 38 for sucking 7 from the tip of nozzle part 25
And a controller 40 for driving the pump 38. This reagent dispensing probe 20
Is moved along a movement path 21 indicated by a dashed line, a reagent is suctioned and collected from a reagent container 17 located there at a reagent suction and collection position 22, and then a reaction container is collected at a reagent dispensing position 23 of the reaction line 5. Dispense the reagent collected in step 4.

A scattered light metering device, which is an analysis unit 1 for an analysis item component having a conventionally known light source unit and measurement unit, is provided rotatably together with the reaction disk 2 at each position accommodating each reaction container. ing.

In the above-described configuration, the reaction vessel rotor 3 is intermittently rotated in the direction of the arrow of the intermittent rotation direction 15 at a constant interval for one pitch. The reaction container 4 sent to the sample dispensing position 14 by the intermittent feeding of the reaction container rotor 3
During the stop period of the reaction container rotor 3, a sample is dispensed from the sample container 7 at the sample aspirating and sampling position 13 by the sample dispenser 9.

The sample dispenser 9 includes the sample dispensing probe 1
When the sample dispensing probe 10 is inserted into the sample container 7 located at the sample aspirating / sampling position 13 of the sampling table 8 and the sample dispensing probe 10 is After aspiration and sampling, the sample dispensing probe 10 is raised, and subsequently, the sample dispensing probe 10
The probe is moved horizontally to the reagent dispensing position 23 of the reaction line 5 along the moving path 11 of
Is lowered, and the collected sample is discharged into the reaction container 4 located there, thereby dispensing the sample.

The sample dispensing probe 10 that has finished dispensing the sample is raised and moved horizontally to the sample aspirating and sampling position 13. At the same time as moving the sample dispensing probe 10, the sampling table 8 is rotated to move the next sample container 7 to be dispensed to the sample aspirating and sampling position 13, and the sample dispensing probe 10 performs the next sample dispensing operation. I do. The reaction container 4 into which the sample has been dispensed in this way intermittently moves along the reaction line 5 in the direction of the arrow of the intermittent rotation direction 15 and is sent to the reagent dispensing position 23 where the reagent dispenser is dispensed. The reagent container 17 at the reagent suction / collection position 22 of the reagent tray 18 by the
Is dispensed into the reaction container 4.

In this embodiment, the reagent dispenser 19 is
The reagent dispensing probe 20 is moved along the movement path 21 and lowered at the reagent suction / collection position 22 of the reagent tray 18, and the reagent dispensing probe 20 is inserted into the reagent container 17 located there. The reagent dispensing probe 20 is the reagent container 1
7, the pump 38 is driven by the controller 40 of FIG. 2 to make the inside of the reagent dispensing probe 20 a negative pressure, and a certain amount of the reagent 37 is sucked and collected. After moving upward from the inside and stopping, the controller 38 further drives the pump 38 to further increase the negative pressure in the reagent dispensing probe 20 for a certain period of time to suck the reagent 37 as shown in FIG. The constant-temperature water is sucked into the circulation range of 36. The constant temperature water 36 is supplied in advance to a supply tank (not shown).
The temperature is controlled within a certain range. Then, it moves to the reagent dispensing position 23 of the reaction line 5 in the horizontal direction along the movement path 21 of FIG. 1, and when it reaches the reagent dispensing position 23, the reagent dispensing nozzle unit 20 is lowered. The collected reagent is discharged to the reaction container 4 located at the position (1) to dispense the reagent. During this time, the entire reagent sucked into the reagent dispensing probe 20 is heated so as to be surrounded by the circulating constant-temperature water 36 so that heat transfer from the constant-temperature water 36 is efficiently performed and the reagent is moved. It is heated to the target temperature without being affected by the environmental temperature during the period.

The reaction container 4 into which the reagent has been dispensed as described above,
It moves intermittently in the direction of the arrow of the intermittent rotation direction 15 along the reaction line 5, but for the reaction container 4 into which the reagent has been dispensed, the scattered light amount is measured by the scattered light amount measuring means provided in each of the reaction container storage sections. Is measured. The sample dispensing probe 10 and the reagent dispensing probe 20 need to be washed every time a sample or a reagent to be handled is changed. However, since the form thereof is not limited by the present invention, the description is omitted. ing. In addition, the description of the disposal unit of the reaction container in which the reacted sample is stored and the supply unit of a new reaction container are also omitted.

When the reagent 37 is dispensed from the reagent container 17 to the reaction container 4 by the reagent dispensing probe 20 as described above, the blood coagulation analyzer dispenses a certain amount of the reagent 37 which has been kept cool and warm. After aspirating to the injection probe 20, the whole reagent is re-aspirated and pulled up so as to be close to the circulation range of the constant temperature water 36 of the reagent dispensing probe, and the entire reagent is efficiently preheated with the constant temperature water 36 during the transfer period. The present invention is characterized in that the entire reagent is heated to a target temperature to maintain the accuracy in the reaction vessel 4, and is not limited to the configuration of the present embodiment. For example, the sample dispensing unit and the reagent dispensing unit can be integrated into one place, or the sample dispensing probe and the reagent dispensing probe can be integrated as one liquid dispenser.

[0018]

The blood coagulation analyzer of the present invention is configured as described above, and even if the ambient temperature of the device changes, the total amount of the reagent sucked into the reagent dispensing probe is close to the constant temperature water. Since the temperature is controlled to the target temperature, a blood coagulation analyzer having excellent reproducibility regardless of the outside air temperature and the reagent temperature can be obtained.

[Brief description of the drawings]

FIG. 1 is a schematic configuration diagram of a blood coagulation analyzer according to an embodiment of the present invention.

FIG. 2 is a cross-sectional view of the reagent dispensing probe of the present invention in a state where a reagent is sucked.

FIG. 3 is a cross-sectional view of a conventional blood coagulation analyzer with a reagent dispensing probe in a reagent suction state.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Analysis part 18 ... Reagent tray 2 ... Reaction disk 19 ... Reagent dispenser 3 ... Reaction container rotor 20, 35 ... Reagent dispensing probe 4 ... Reaction container 20a ... Inlet 5 ... Reaction line 20b ... Outlet 6 ... Sample dispensing Unit 21: moving path 7: sample container 22: reagent suction and collection position 8: sampling table 23: reagent dispensing position 9: sample dispenser 25: nozzle unit 10: sample dispensing probe 26: outer cylinder 11: moving path 36 ... constant temperature water 13 ... sample aspirating and sampling position 37 ... reagent 14 ... sample dispensing position 38 ... pump 15 ... intermittent rotation direction 39 ... piping 16 ... reagent dispensing part 40 ... controller 17 ... reagent container

Claims (1)

[Claims] 1. A sample dispensing section for dispensing a sample into a reaction container via a sample dispensing probe for aspirating and collecting a sample contained in a sample container, and a sample dispensing unit accommodated in a reagent container being kept cool. A reagent dispensing unit for dispensing the reagent into the reaction container via a reagent dispensing probe that sucks the reagent and heats the solution with constant temperature water, and an analysis for measuring the reaction characteristics of the reaction solution in the reaction container In the blood coagulation analyzer provided with a section, a means is provided in which the reagent is aspirated to the reagent dispensing probe and then air is further sucked, so that the whole sucked reagent is heated close to constant temperature water. A blood coagulation analyzer characterized by the above-mentioned.