CN109234203A - Biological feedstuff rush digestion composite bacteria agent and its application - Google Patents
Biological feedstuff rush digestion composite bacteria agent and its application Download PDFInfo
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Abstract
The present invention provides a kind of biological feedstuff rush digestion composite bacteria agents, based on dry weight parts by weight, it is composed of the following components: to produce cellulosome microbial bacterial agent 40-80 parts by weight, lactobacillus microbial inoculum 15-37 parts by weight, 5-25 parts of bacillus subtilis microbial agent parts by weight, it is uniformly mixed, obtains composite bacteria agent.The production cellulosome microorganism is Clostridium thermocellum, the molten fine clostridium of yellow or molten fiber vacation bacteroid.The present invention also provides the methods for preparing lignocellulosic base biological feedstuff using the composite bacteria agent.Lignocellulosic base biological feedstuff is prepared using the composite bacteria agent, not only promotes the digestion and absorption of food, significantly improves laying rate, reduce feedstuff-meat ratio, pathogenic bacteria growth and breeding can also be inhibited, reduces disease incidence.In addition, chicken disease incidence (reduction), laying rate of laying hen (raising), broiler chicken feedstuff-meat ratio (raising) and other effects are significantly better than basic chicken feed.
Description
Technical field
The invention belongs to biological feedstuff technical field, it is related to specifically relating to using the technology of lignocellulosic preparation chicken feed
And a kind of biological feedstuff rush digests composite bacteria agent and its composite bacteria agent preparation is used to promote to digest, raising laying rate, drop
The method of the feed of low feedstuff-meat ratio.
Background technique
The critical issue of aquaculture development is the general level of the health and production capacity of animal.As the most common poultry it
One, chicken has become the stabilization source of mankind's meat egg food.Therefore how to accelerate the growth of chicken, improve egg laying performance, reduce
The rate of falling ill is the hot spot that home poultry raising field is paid close attention to for a long time.
Lignocellulose-like biomass is present on earth renewable biomass resources the most abundant.China is agriculture big
State, annual agricultural crop straw yield are more than 900,000,000 tons.Lignocellulose-like biomass is used for the preparation of feed, was both able to achieve
The high-value-use of abandoned biomass, and it is high to can solve feed price, the problem of relying on import.In lignocellulosic it is main at
It is divided into the crude fibres such as cellulose, hemicellulose and lignin；And not only without digesting coarse-fibred enzyme in the alimentary canal of chicken, and
Alimentary canal is short, gastric volume is small, therefore feed, by alimentary canal fast speed, 4 hours are discharged after general feeds feeding, and 24
Hour drains indigestible part, absorbs to nutrient digestion incomplete.Therefore, a large amount of coarse-fibred presence will cause feeding
The waste of material.
Application for a patent for invention 201710258656.7 discloses " a kind of solid fermentation stalk chicken feed and preparation method thereof ",
Corn stover and budding fungus composite zymocyte are carried out mesopilous organisms fermentation by this application, are realized to egg thick in corn stover
The utilization of white, short chain cellulose and carbohydrate.Patent of invention ZL201310651866.4 is disclosed " to be produced using fermented maize stalk
The method of efficient broiler fodder ", the patent is by corn stover through ammonium hydroxide-steam treatment, and then addition produces single cell protein
Fungi and yeast and producing enzyme bacterium carry out the processing such as ferment, to improve the utilization rate of corn stover.Patent of invention
ZL201510414300.9 is disclosed " a kind of technique that agricultural crop straw is converted into pig chicken feed resource ", the patent pass through by
Stalk is pre-processed, and is then added cellulase and is hydrolyzed, and is realized and is discharged the sugar in crude fibre from condensate, and
The purpose that carbohydrate as feed adds.Application for a patent for invention 201710767486.5 discloses a kind of " explosion-enzymatic hydrolysis
The biological straw particle chicken feed of processing " carries out fiber after this application is by carrying out alkaline process and explosion pretreatment for corn stover
Element enzymatic hydrolysis, is then mixed with granular pattern chicken feed with basic chicken feed.However, above-mentioned processing method still has following ask
Topic: (1) crude fibre insufficient hydrolysis, without fundamentally solving the problems, such as the decline of digestion and absorption efficiency；(2) processing cost
Height, less economical, the prospect of practical application is not still good enough in the industry.
Clostridium thermocellum etc. produces the efficient degradation vigor that cellulosome microorganism naturally has lignocellulosic.Therefore, it relies on
Crude fibre may be implemented to the Efficient Conversion of sugar in cellulosome and the saccharification technology for producing cellulosome microorganism.For example, research
In the work of personnel before this using Clostridium thermocellum realize microcrystalline cellulose effectively hydrolyzing (Zhang, J., S.Liu, R.Li,
W.Hong,Y.Xiao,Y.Feng,Q.Cui and Y.-J.Liu(2017)."Efficient whole-cell-
catalyzing cellulosesaccharification using engineered Clostridium
Thermocellum. " Biotechnol Biofuels 10 (1): 124.), but lignocellulosic substrate it is not applied to also
Hydrolysis and lignocellulosic base feed exploitation and preparation, and by the way that cellulosome microorganism will be produced as feed addictive
Prepare chicken feed, if having the function of that digestion, raising laying rate, reduction feedstuff-meat ratio is promoted to still need to inquire into.
Summary of the invention
It is wooden with promoting digestion composite bacteria agent and the composite bacteria agent being used to prepare that the present invention provides a kind of biological feedstuffs
The method of cellulose base biological feedstuff.Lignocellulosic base biological feedstuff is prepared using the composite bacteria agent, not only promotes food
Digestion and absorption, significantly improve laying rate, reduce feedstuff-meat ratio, pathogenic bacteria growth and breeding can also be inhibited, reduce
Disease incidence.
Technical solution of the present invention: biological feedstuff is with digestion composite bacteria agent is promoted, based on dry weight parts by weight, by following components
Composition: cellulosome microbial bacterial agent 40-80 parts by weight, lactobacillus microbial inoculum 15-37 parts by weight, 5-25 parts of bacillus subtilises are produced
Microbial inoculum parts by weight are uniformly mixed, obtain composite bacteria agent.
The production cellulosome microbial bacterial agent is prepared by the following method: according to the solid and liquid weight of 1:20-1:50
Pretreated lignocellulosic material and dextrose culture-medium are added into reaction unit for volume ratio, according to 5-10% volume
The inoculum concentration inoculation of score produces the microorganism of cellulosome, then mixes under 42-65 DEG C, the speed conditions of 100-150r/min
Uniformly, regular check container inner pressure and deflate terminate to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, acquired
Solid phase be produce cellulosome microbial bacterial agent.This method makees the degradation of lignocellulosic using production cellulosome microorganism
With acquisition lignocellulosic base soluble sugar；And using probiotics such as its collaboration culture bacillus subtilis, lactobacillus, promote
The digestion and absorption of food；Laying rate can significantly be improved, reduce feedstuff-meat ratio.Meanwhile producing cellulosome microorganism and benefit
The products such as lactic acid, acetic acid can also be produced in raw bacterium growth course, reduce the pH value of raw material, to inhibit the life of pathogenic bacteria
Long breeding, reduces disease incidence.
Wherein, the production cellulosome microorganism is Clostridium thermocellum, the molten fine clostridium of yellow or molten fiber vacation bacteroid.Institute
The pretreatment stated is one of alkaline process, acid system, hydro-thermal method, steam explosion method and sulfonation method preconditioning technique or a variety of combinations.Institute
The lignocellulosic material stated is one of corn stover, wheat straw, corncob, maize peel, straw or a variety of combinations.It is described
Dextrose culture-medium be that dipotassium hydrogen phosphate 2.9g/L, potassium dihydrogen phosphate 1.5g/L, urea 0.8g/L, chlorination are added in every liter of water
Calcium 0.1g/L, magnesium chloride 1.8g/L, ferrous sulfate 0.0005g/L, vulcanized sodium 2g/L, corn pulp 4g/L, trisodium citrate 2g/L,
Glucose 1-20g/L, pH 6.5-7.5.The reaction unit is the anaerobic fermentation equipment with agitating paddle.
The composite bacteria agent is used to prepare rush digestion biology by the biological feedstuff application for promoting digestion composite bacteria agent
Feed.
Promote the preparation method of digestion biological feedstuff, comprising the following steps:
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:20-1:50, being filled to reaction
The pretreated lignocellulosic material of middle addition and dextrose culture-medium are set, is inoculated with according to the inoculum concentration of 5-10% volume fraction
The microorganism of cellulosome is produced, is then uniformly mixed under 42-65 DEG C, the speed conditions of 100-150r/min；Regular check is held
It pressure and deflates in device, terminates to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce fiber
Corpusculum microbial bacterial agent.
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent that step (1) obtains is weighed
40-80 parts by weight, lactobacillus microbial inoculum 15-37 parts by weight, 5-25 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain
Composite bacteria agent；The production cellulosome microorganism is Clostridium thermocellum, the molten fine clostridium of yellow or molten fiber vacation bacteroid.
(3) promote the preparation of digestion biological feedstuff: by the production cellulosome microbial bacterial agent or step (2) of step (1) preparation
The composite bacteria agent of preparation is added in basal feed according to mass fraction 7-50%, stirs evenly, and obtains promoting to digest reinforced life
Object feed.The basis chicken feed is composed of the following components: 65 parts by weight Corn, 20 parts by weight soya-bean cakes, 3 parts by weight fish meal, and 1.8
Parts by weight bone meal, 0.2 parts by weight salt, 10 parts by weight viridescent dish leafs.
Beneficial effects of the present invention:
(1) lignocellulosic base biological feedstuff is prepared using the composite bacteria agent, using production cellulosome microorganism to wood
The degradation of matter cellulose obtains lignocellulosic base soluble sugar, and utilizes its collaboration culture bacillus subtilis, newborn bar
The probiotics such as bacterium promote the digestion and absorption of food, to significantly improve laying rate, reduce feedstuff-meat ratio.
(2) lignocellulosic base biological feedstuff is prepared using the composite bacteria agent, using produce cellulosome microorganism and
The products such as lactic acid, the acetic acid produced during growth of probiotics, reduce the pH value of raw material, inhibit pathogenicity thin to realize
Bacterium growth and breeding, the effect for reducing disease incidence.
(2) lignocellulosic base biological feedstuff is prepared using the composite bacteria agent in the present invention, with control basis chicken feed
It compares, chicken disease incidence is not less than 90% lower than 1.8%, laying rate of laying hen, and the feedstuff-meat ratio to raise table hens can be down to 1.41, than basis
Chicken feed reduces by 25.8%, and significant effect is better than basic chicken feed.
Specific embodiment
The present invention will be further explained with reference to the examples below.
Embodiment 1: promote the preparation of digestion biological feedstuff
(1) preparation of cellulosome microbial bacterial agent is produced:
According to the solid and liquid weight volume ratio of 1:20, the pretreated corn stover of alkaline process and grape are added into reaction unit
Sugar culture-medium is inoculated with Clostridium thermocellum according to the inoculum concentration of 5% volume fraction, then under 55 DEG C, the speed conditions of 120r/min
It is uniformly mixed；Regular check container inner pressure is simultaneously deflated, and terminates to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation,
Obtained solid phase is to produce cellulosome microbial bacterial agent.
The dextrose culture-medium is that dipotassium hydrogen phosphate 2.9g/L, potassium dihydrogen phosphate 1.5g/L, urine are added in every liter of water
Plain 0.8g/L, calcium chloride 0.1g/L, magnesium chloride 1.8g/L, ferrous sulfate 0.0005g/L, vulcanized sodium 2g/L, corn pulp 4g/L, lemon
Lemon acid trisodium 2g/L, glucose 1-20g/L, pH 6.5-7.5.The reaction unit is the anaerobic fermentation equipment with agitating paddle.
(2) preparation of composite bacteria agent:
According to dry weight weight, 40 parts by weight of production cellulosome microbial bacterial agent, the lactobacillus microbial inoculum that step (1) obtains are weighed
35 parts by weight, 25 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain composite bacteria agent.
(3) promote the preparation of digestion biological feedstuff:
Composite bacteria agent prepared by step (2) is added in basal feed according to mass fraction 30%, stirs evenly, obtains
Promote to digest reinforced biological feedstuff.The basis chicken feed is composed of the following components: 65 parts by weight Corn, 20 parts by weight soya-bean cakes, and 3
Parts by weight fish meal, 1.8 parts by weight bone meal, 0.2 parts by weight salt, 10 parts by weight viridescent dish leafs.
Embodiment 2: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:20, adding into reaction unit
Enter the pretreated corncob of sulfonation method and dextrose culture-medium, according to the molten fine shuttle of the inoculum concentration inoculation yellow of 5% volume fraction
Then bacterium is uniformly mixed under 60 DEG C, the speed conditions of 100r/min；Regular check container inner pressure is simultaneously deflated, when in container
Terminate to cultivate when no positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacterial agent.
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent that step (1) obtains is weighed
50 parts by weight, 37 parts by weight of lactobacillus microbial inoculum, 13 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain composite bacteria agent.
(3) promote the preparation of digestion biological feedstuff: composite bacteria agent prepared by step (2) is added to according to mass fraction 7%
It in basal feed, stirs evenly, obtains promoting to digest reinforced biological feedstuff.
Embodiment 3: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:30, adding into reaction unit
Enter the pretreated maize peel of hydro-thermal method and dextrose culture-medium, it is quasi- to be inoculated with molten fiber vacation according to the inoculum concentration of 10% volume fraction
Then bacillus is uniformly mixed under 55 DEG C, the speed conditions of 150r/min；Regular check container inner pressure is simultaneously deflated, and container is worked as
Terminate to cultivate when interior no positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacterial agent.
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent that step (1) obtains is weighed
60 parts by weight, 20 parts by weight of lactobacillus microbial inoculum, 20 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain composite bacteria agent.
(3) promote the preparation of digestion biological feedstuff: composite bacteria agent prepared by step (2) is added to according to mass fraction 15%
It in basal feed, stirs evenly, obtains promoting to digest reinforced biological feedstuff.
Embodiment 4: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:40, adding into reaction unit
Wheat straw and dextrose culture-medium after entering alkaline process and steam explosion method combined pretreatment, according to the inoculum concentration vaccinal fever of 10% volume fraction
Then fine clostridium is uniformly mixed under 60 DEG C, the speed conditions of 150r/min；Regular check container inner pressure is simultaneously deflated, and appearance is worked as
Terminate to cultivate when in device without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacterial agent.
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent that step (1) obtains is weighed
70 parts by weight, 15 parts by weight of lactobacillus microbial inoculum, 15 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain composite bacteria agent.
(3) promote the preparation of digestion biological feedstuff: composite bacteria agent prepared by step (2) is added to according to mass fraction 15%
It in basal feed, stirs evenly, obtains promoting to digest reinforced biological feedstuff.
Embodiment 5: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:50, adding into reaction unit
Straw and dextrose culture-medium after entering sulfonation method and hydro-thermal method combined pretreatment are inoculated with according to the inoculum concentration of 10% volume fraction
Then Clostridium thermocellum is uniformly mixed under 60 DEG C, the speed conditions of 150r/min；Regular check container inner pressure is simultaneously deflated, when
Terminate to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacterial agent.
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent that step (1) obtains is weighed
80 parts by weight, 15 parts by weight of lactobacillus microbial inoculum, 5 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain composite bacteria agent.
(3) promote the preparation of digestion biological feedstuff: composite bacteria agent prepared by step (2) is added to according to mass fraction 20%
It in basal feed, stirs evenly, obtains promoting to digest reinforced biological feedstuff.
Embodiment 6: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:20, adding into reaction unit
Corn stover and dextrose culture-medium after entering sulfonation method-hydro-thermal combined pretreatment are inoculated with according to the inoculum concentration of 5% volume fraction
Then Clostridium thermocellum is uniformly mixed under 60 DEG C, the speed conditions of 100r/min；Regular check container inner pressure is simultaneously deflated, when
Terminate to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacterial agent.
(2) promote the preparation of digestion biological feedstuff: by the production cellulosome microbial bacterial agent of step (1) preparation according to quality point
Number 7% is added in basal feed, stirs evenly, and obtains promoting to digest reinforced biological feedstuff.
Embodiment 7: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:20, adding into reaction unit
Corn stover and dextrose culture-medium after entering sulfonation method-hydro-thermal combined pretreatment are inoculated with according to the inoculum concentration of 5% volume fraction
Then the molten fine clostridium of yellow is uniformly mixed under 65 DEG C, the speed conditions of 100r/min；Regular check container inner pressure is simultaneously put
Gas terminates to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacteria
Agent.
(2) promote the preparation of digestion biological feedstuff: by the production cellulosome microbial bacterial agent of step (1) preparation according to quality point
Number 30% is added in basal feed, stirs evenly, and obtains promoting to digest reinforced biological feedstuff.
Embodiment 8: promote the preparation of digestion biological feedstuff
Unlike the first embodiment,
(1) it produces the preparation of cellulosome microbial bacterial agent: according to the solid and liquid weight volume ratio of 1:20, adding into reaction unit
Corn stover and dextrose culture-medium after entering sulfonation method-hydro-thermal combined pretreatment are inoculated with according to the inoculum concentration of 5% volume fraction
Then molten fiber vacation bacteroid is uniformly mixed under 42 DEG C, the speed conditions of 100r/min；Regular check container inner pressure is simultaneously put
Gas terminates to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, and obtained solid phase is to produce cellulosome microbial bacteria
Agent.
(2) promote the preparation of digestion biological feedstuff: by the production cellulosome microbial bacterial agent of step (1) preparation according to quality point
Number 30% is added in basal feed, stirs evenly, and obtains promoting to digest reinforced biological feedstuff.
Embodiment 9: laying hen feeding experiment
Blue brown laying hen 300 of the sea of the close health of 30 week old production performances is selected in test, is randomly divided into 6 groups, every component 5
It repeats, 10 chickens of each repetition.Test group 1-5 feeds the lignocellulosic base biological feedstuff of embodiment 1-5 preparation, blank respectively
Control group only feeds Basic drawing.Every chicken feeds 150g feed daily, and twice, free water feeds 30 altogether for feeding daily
It.
Embodiment 10: broiler feeding test
White meat-type chickens 300 of the close health of 30 age in days production performances are selected in test, are randomly divided into 6 groups, 5 weights of every component
It is multiple, 10 chickens of each repetition.Test group 1-5 feeds the lignocellulosic base biological feedstuff of embodiment 1-5 preparation, blank pair respectively
According to group, Basic drawing is only fed.Feeding method are as follows: be freely eaten for first week, free water；Second week rise, before daily daybreak, in
Turn off the light after noon and darkness 2-3 hours, not feeding, do not drink water, remaining time turns on light free choice feeding, free water.It feeds 30 days altogether,
Each broiler growth situation is weighed and recorded, feedstuff-meat ratio is calculated.
Index parameter after the rush digestion biological feedstuff feeding chicken of 1. embodiment 1-5 of table preparation
As shown in Table 1, embodiment 1-5 promotees digestion feed using composite bacteria agent preparation, using rush digestion feed feeding
Laying hen and broiler chicken, disease incidence 0-2% reduce 2-4%, or even accomplish zero disease incidence compared with the control group；Wherein, laying hen
Laying rate is 92-96%, compared with the control group, increases 4-8 percentage points；The feedstuff-meat ratio of broiler chicken is 1.41-1.71, and is compareed
Group is compared, and 10-25.8% has been reduced.
Embodiment 6-8 digests feed using the rush for producing the preparation of cellulosome microbial bacterial agent, digests feed using the rush
Feeding laying hen and broiler chicken, disease incidence 2% slightly reduce compared with the control group；Wherein, the laying rate of laying hen is 90-92%,
Compared with the control group, 2-4 percentage points, slightly below embodiment 1-5 are increased；The feedstuff-meat ratio of broiler chicken is 1.68-1.74, and is compareed
Group is compared, and 8.4-11.6% is reduced, equally slightly below embodiment 1-5.
This explanation, compared with only with the rush digestion feed of cellulosome microbial bacterial agent preparation is produced, using described compound
The rush of microbial inoculum preparation digests feed, and effect is more preferable.This is because being obtained using cellulosome microbial degradation lignocellulosic is produced
Lignocellulosic base soluble sugar, collaboration cultivated the probiotics such as bacillus subtilis, lactobacillus, further promoted food
Digestion and absorption, to significantly improve laying rate, reduce feedstuff-meat ratio；And utilize production cellulosome microorganism and probiotics
Lactic acid, the acetic acid produced in growth course has achieved the purpose that inhibit pathogenic bacteria growth and breeding, has reduced disease incidence.
Claims (9)
1. biological feedstuff rush digestion composite bacteria agent, it is characterised in that: composed of the following components based on dry weight parts by weight: to produce
Cellulosome microbial bacterial agent 40-80 parts by weight, lactobacillus microbial inoculum 15-37 parts by weight, 5-25 parts of bacillus subtilis microbial agent weights
Part is measured, is uniformly mixed, obtains composite bacteria agent；
The production cellulosome microbial bacterial agent is prepared by the following method: according to the solid and liquid weight volume of 1:20-1:50
Than pretreated lignocellulosic material and dextrose culture-medium being added into reaction unit, according to 5-10% volume fraction
Inoculum concentration inoculation produce the microorganism of cellulosome, then mixed under 42-65 DEG C, the speed conditions of 100-150r/min equal
It is even, regular check container inner pressure and deflate terminate to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, obtained
Solid phase is to produce cellulosome microbial bacterial agent.
2. biological feedstuff according to claim 1 rush digestion composite bacteria agent, it is characterised in that: the production cellulosome
Microorganism is Clostridium thermocellum, the molten fine clostridium of yellow or molten fiber vacation bacteroid.
3. biological feedstuff according to claim 2 rush digestion composite bacteria agent, it is characterised in that: the pretreatment is alkali
One of method, hydro-thermal method, steam explosion method and sulfonation method preconditioning technique or a variety of combinations.
4. the preparation method according to claim 2 for promoting digestion biological feedstuff, it is characterised in that: the lignocellulosic
Raw material is one of corn stover, wheat straw, corncob, maize peel, straw or a variety of combinations.
5. the preparation method according to claim 2 for promoting digestion biological feedstuff, it is characterised in that: the glucose culture
Base is that dipotassium hydrogen phosphate 2.9g/L, potassium dihydrogen phosphate 1.5g/L, urea 0.8g/L, calcium chloride 0.1g/L, chlorine are added in every liter of water
Change magnesium 1.8g/L, ferrous sulfate 0.0005g/L, vulcanized sodium 2g/L, corn pulp 4g/L, trisodium citrate 2g/L, glucose 1-
20g/L、pH 6.5-7.5。
6. the preparation method of rush digestion biological feedstuff described in -5 any one according to claim 1, it is characterised in that: described anti-
Answering device is the anaerobic fermentation equipment with agitating paddle.
7. the biological feedstuff application for promoting digestion composite bacteria agent as described in claim 1-5 any one, it is characterised in that: will
The composite bacteria agent is used to prepare rush digestion biological feedstuff.
8. promoting the preparation method of digestion biological feedstuff, it is characterised in that: the following steps are included:
(1) preparation of cellulosome microbial bacterial agent is produced: according to the solid and liquid weight volume ratio of 1:20-1:50, into reaction unit
Pretreated lignocellulosic material and dextrose culture-medium is added, is then mixed under the speed conditions of 100-150r/min
Uniformly；Regular check container inner pressure and deflate terminate to cultivate when in container without positive pressure；Centrifugation is separated by solid-liquid separation, acquired
Solid phase be produce cellulosome microbial bacterial agent；
(2) preparation of composite bacteria agent: according to dry weight weight, the production cellulosome microbial bacterial agent 40-80 that step (1) obtains is weighed
Parts by weight, lactobacillus microbial inoculum 15-37 parts by weight, 5-25 parts of bacillus subtilis microbial agent parts by weight are uniformly mixed, obtain compound bacteria
Agent；The production cellulosome microorganism is Clostridium thermocellum, the molten fine clostridium of yellow or molten fiber vacation bacteroid.
(3) promote the preparation of digestion biological feedstuff: is prepared by the production cellulosome microbial bacterial agent of step (1) preparation or step (2)
Composite bacteria agent be added in basal feed according to mass fraction 7-30%, stir evenly, obtain promote digestion it is reinforced biology raise
Material.
9. it is according to claim 8 promote digestion biological feedstuff preparation method, it is characterised in that: it is described basis chicken feed by
Following components composition: 65 parts by weight Corn, 20 parts by weight soya-bean cakes, 3 parts by weight fish meal, 1.8 parts by weight bone meal, 0.2 parts by weight food
Salt, 10 parts by weight viridescent dish leafs.
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| CN103892104A (en) * | 2012-12-28 | 2014-07-02 | 天津市现代天骄农业科技有限公司 | Broiler feed with effects of promoting digestion and enhancing immunity |
| CN104336412A (en) * | 2014-11-11 | 2015-02-11 | 武汉轻工大学 | Method for producing monogastric animal feed by fermenting pleurotus eryngii bran |
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| CN103892104A (en) * | 2012-12-28 | 2014-07-02 | 天津市现代天骄农业科技有限公司 | Broiler feed with effects of promoting digestion and enhancing immunity |
| CN104336412A (en) * | 2014-11-11 | 2015-02-11 | 武汉轻工大学 | Method for producing monogastric animal feed by fermenting pleurotus eryngii bran |
| CN108118020A (en) * | 2018-01-16 | 2018-06-05 | 中国科学院青岛生物能源与过程研究所 | Culture medium, preparation and its application of cellulose degradation microorganism |
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