CN109234203B - Digestion-promoting composite microbial agent for biological feed and application thereof - Google Patents
Digestion-promoting composite microbial agent for biological feed and application thereof Download PDFInfo
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Abstract
The invention provides a digestion-promoting composite microbial inoculum for biological feed, which comprises the following components in parts by weight: 40-80 parts of cellulosome-producing microbial agent, 15-37 parts of lactobacillus agent and 5-25 parts of bacillus subtilis agent, and uniformly mixing to obtain the composite microbial agent. The cellulosome-producing microorganism is clostridium thermocellum, clostridium thermocellum yellow or pseudomonas fibrinolyticum. The invention also provides a method for preparing lignocellulose-based biological feed by using the composite microbial inoculum. The lignocellulose-based biological feed prepared by the composite microbial inoculum not only promotes digestion and absorption of food, remarkably improves the laying rate, reduces the feed conversion ratio, but also can inhibit growth and reproduction of pathogenic bacteria and reduce the morbidity. In addition, the effects of chicken morbidity (reduction), laying rate (improvement), broiler feed conversion (improvement) and the like are obviously superior to those of basic chicken feed.
Description
Technical Field
The invention belongs to the technical field of biological feed, relates to a technology for preparing chicken feed by adopting lignocellulose, and particularly relates to a digestion-promoting composite microbial inoculum for biological feed and a method for preparing feed for promoting digestion, improving laying rate and reducing feed conversion ratio by adopting the composite microbial inoculum.
Background
A key issue in the development of the breeding industry is the health level and productivity of the animals. As one of the most common poultry, chickens have become a stable source of human meat and egg food. Therefore, how to accelerate the growth of chicken, improve the egg laying capacity and reduce the disease rate is a hot spot of long-term attention in the field of poultry feeding.
Lignocellulosic biomass is the most abundant renewable biomass resource in the earth. China is a big agricultural country, and the annual crop straw yield exceeds 9 hundred million tons. The lignocellulose biomass is used for preparing the feed, so that high-value utilization of the waste biomass can be realized, and the problems of high feed price and dependence on import can be solved. The main components of the lignocellulose are crude fibers such as cellulose, hemicellulose, lignin and the like; the feed is discharged in 4 hours after being generally eaten, and the indigestible part is excreted in 24 hours, so that the digestion and absorption of nutrient substances are incomplete. Therefore, the presence of a large amount of coarse fibers causes a waste of feed.
The invention patent application 201710258656.7 discloses a solid fermentation straw chicken feed and a preparation method thereof, which carries out mesophilic biofermentation on corn straws and yeast fungus composite zymocyte and realizes the utilization of crude protein, short-chain cellulose and sugar in the corn straws. The invention patent ZL201310651866.4 discloses a method for producing high-efficiency broiler feed by utilizing fermented corn straws, which is characterized in that the corn straws are treated by ammonia water-steam, and then are added with fungi and yeast for producing single-cell protein and enzyme-producing bacteria for fermentation and other treatments, so that the utilization rate of the corn straws is improved. The invention patent ZL201510414300.9 discloses a process for converting crop straws into pig and chicken feed resources, which achieves the purposes of releasing sugar in crude fiber from polymers and adding the sugar as carbohydrate of feed by pretreating the straws and then adding cellulase for hydrolysis. The invention patent application 201710767486.5 discloses a biological straw granulated chicken feed processed by blasting and enzymolysis, which is prepared by carrying out alkaline method and blasting pretreatment on corn straws, then carrying out cellulose enzymolysis, and then mixing the corn straws with a basic chicken feed. However, the above processing method still has the following problems: (1) the crude fiber is not hydrolyzed fully, and the problem of the reduction of the digestion and absorption efficiency is not solved fundamentally; (2) high treatment cost, poor economy and poor prospect of practical application in industry.
The cellulosome-producing microorganisms such as clostridium thermocellum and the like have high-efficiency degradation activity of lignocellulose. Thus, saccharification techniques that rely on cellulosomes as well as on cellulosome-producing microorganisms can achieve efficient conversion of crude fiber to sugar. For example, researchers have been working to achieve Efficient hydrolysis of microcrystalline cellulose using clostridium thermocellum (Zhang, j., s.liu, r.li, w.hong, y.xiao, y.feng, q.cui and y. -j.liu (2017). "effective hold-cell-catalyzed cellulose thermal cell." Biotechnol Biofuels 10(1):124. ") but have not been applied to hydrolysis of lignocellulosic substrates and development and preparation of lignocellulosic feeds, and whether chicken feeds prepared by using cellulosome-producing microorganisms as feed additives have the effects of promoting digestion, increasing egg production rate, and decreasing feed conversion ratio still needs to be investigated.
Disclosure of Invention
The invention provides a digestion-promoting composite microbial inoculant for biological feed and a method for preparing lignocellulose-based biological feed by using the composite microbial inoculant. The lignocellulose-based biological feed prepared by the composite microbial inoculum not only promotes digestion and absorption of food, remarkably improves the laying rate, reduces the feed conversion ratio, but also can inhibit growth and reproduction of pathogenic bacteria and reduce the morbidity.
The technical scheme of the invention is as follows: the digestion-promoting composite microbial inoculum for the biological feed comprises the following components in parts by weight in dry weight: 40-80 parts of cellulosome-producing microbial agent, 15-37 parts of lactobacillus agent and 5-25 parts of bacillus subtilis agent, and uniformly mixing to obtain the composite microbial agent.
The cellulosome-producing microbial agent is prepared by the following method: adding pretreated lignocellulose raw material and glucose culture medium into a reaction device according to the solid-liquid weight-volume ratio of 1:20-1:50, inoculating the microorganism producing the cellulosome according to the inoculation amount of 5-10% volume fraction, then uniformly mixing at 42-65 ℃ and the rotation speed of 100-150r/min, checking the pressure in the container at regular time, and releasing gas to finish the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome. The method utilizes the degradation effect of cellulosome-producing microorganisms on lignocellulose to obtain lignocellulose-based soluble sugar; and the probiotics such as bacillus subtilis, lactobacillus and the like are cooperatively cultured by utilizing the probiotics, so that the digestion and absorption of food are promoted; can obviously improve the laying rate and reduce the feed conversion ratio. Meanwhile, products such as lactic acid, acetic acid and the like can be produced in the growth process of the cellulosome-producing microorganisms and the probiotics, and the pH value of the raw materials is reduced, so that the growth and the propagation of pathogenic bacteria are inhibited, and the morbidity is reduced.
Wherein the cellulosome-producing microorganism is Clostridium thermocellum, Clostridium flavum or Pseudobacteroides cellulolyticus. The pretreatment is one or a combination of more of alkaline method, acid method, hydrothermal method, steam explosion method and sulfonation method pretreatment technologies. The lignocellulose raw material is one or a combination of a plurality of corn stalks, wheat straws, corncobs, corn husks and rice straws. The glucose culture medium is prepared by adding 2.9g/L dipotassium hydrogen phosphate, 1.5g/L potassium dihydrogen phosphate, 0.8g/L urea, 0.1g/L calcium chloride, 1.8g/L magnesium chloride, 0.0005g/L ferrous sulfate, 2g/L sodium sulfide, 4g/L corn steep liquor, 2g/L trisodium citrate and 1-20g/L, pH 6.5.5-7.5 glucose per liter of water. The reaction device is an anaerobic fermentation device with a stirring paddle.
The composite microbial inoculum is used for preparing the digestion-promoting biological feed.
The preparation method of the digestion promoting biological feed comprises the following steps:
(1) preparing a cellulosome-producing microbial agent: adding pretreated lignocellulose raw material and glucose culture medium into a reaction device according to the solid-liquid weight-volume ratio of 1:20-1:50, inoculating the cellulosome-producing microorganisms according to the inoculation amount of 5-10% volume fraction, and then uniformly mixing at 42-65 ℃ and the rotation speed of 100-150 r/min; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a complex microbial inoculum: weighing 40-80 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 15-37 parts by weight of a lactobacillus agent and 5-25 parts by weight of a bacillus subtilis agent according to dry weight, and uniformly mixing to obtain a composite microbial agent; the cellulosome-producing microorganism is clostridium thermocellum, clostridium thermocellum yellow or pseudomonas fibrinolyticum.
(3) Preparing a digestion promoting biological feed: and (3) adding the cellulosome-producing microbial agent prepared in the step (1) or the composite microbial agent prepared in the step (2) into the basic feed according to the mass fraction of 7-50%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed. The basic chicken feed consists of the following components: 65 parts of corn, 20 parts of bean cake, 3 parts of fish meal, 1.8 parts of bone meal, 0.2 part of salt and 10 parts of green vegetable leaves.
The invention has the beneficial effects that:
(1) the lignocellulose-based biological feed is prepared by adopting the composite microbial agent, lignocellulose-based soluble sugar is obtained by utilizing the degradation effect of cellulosome-producing microorganisms on lignocellulose, and probiotics such as bacillus subtilis, lactobacillus and the like are synergistically cultured by utilizing the lignocellulose-based soluble sugar to promote the digestion and absorption of food, so that the laying rate is remarkably improved, and the feed conversion ratio is reduced.
(2) The lignocellulose-based biological feed prepared by the composite microbial agent reduces the pH value of the raw materials by using the cellulosome-producing microorganisms and lactic acid, acetic acid and other products produced in the growth process of probiotics, thereby realizing the effects of inhibiting the growth and the reproduction of pathogenic bacteria and reducing the morbidity.
(2) According to the lignocellulose-based biological feed prepared by using the composite microbial inoculum, compared with a control basic chicken feed, the morbidity of chickens is lower than 1.8%, the laying rate of laying hens is not lower than 90%, the feed-meat ratio of raised broilers can be lower than 1.41, the feed-meat ratio is reduced by 25.8% compared with the basic chicken feed, and the effect is obviously better than that of the basic chicken feed.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1: preparation of digestion promoting biological feed
(1) Preparing a cellulosome-producing microbial agent:
adding the corn straw and the glucose culture medium pretreated by the alkaline method into a reaction device according to the solid-liquid weight-volume ratio of 1:20, inoculating clostridium thermocellum according to the inoculation amount of 5% volume fraction, and then uniformly mixing at the rotation speed of 120r/min at 55 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
The glucose culture medium is prepared by adding 2.9g/L dipotassium hydrogen phosphate, 1.5g/L potassium dihydrogen phosphate, 0.8g/L urea, 0.1g/L calcium chloride, 1.8g/L magnesium chloride, 0.0005g/L ferrous sulfate, 2g/L sodium sulfide, 4g/L corn steep liquor, 2g/L trisodium citrate and 1-20g/L, pH 6.5.5-7.5 glucose per liter of water. The reaction device is an anaerobic fermentation device with a stirring paddle.
(2) Preparing a complex microbial inoculum:
weighing 40 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 35 parts by weight of lactobacillus agent and 25 parts by weight of bacillus subtilis agent according to the dry weight, and uniformly mixing to obtain the composite microbial agent.
(3) Preparing a digestion promoting biological feed:
and (3) adding the composite microbial inoculum prepared in the step (2) into a basic feed according to the mass fraction of 30%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed. The basic chicken feed consists of the following components: 65 parts of corn, 20 parts of bean cake, 3 parts of fish meal, 1.8 parts of bone meal, 0.2 part of salt and 10 parts of green vegetable leaves.
Example 2: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding the corn cob and the glucose culture medium pretreated by the sulfonation method into a reaction device according to the solid-liquid weight-volume ratio of 1:20, inoculating yellow clostridium cellulolyticum according to the inoculation amount of 5% volume fraction, and then uniformly mixing at the rotation speed of 100r/min at 60 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a complex microbial inoculum: weighing 50 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 37 parts by weight of lactobacillus agent and 13 parts by weight of bacillus subtilis agent according to the dry weight, and uniformly mixing to obtain the composite microbial agent.
(3) Preparing a digestion promoting biological feed: and (3) adding the compound microbial inoculum prepared in the step (2) into the basic feed according to the mass fraction of 7%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 3: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding the corn bran and the glucose culture medium pretreated by the hydrothermal method into a reaction device according to the solid-liquid weight-volume ratio of 1:30, inoculating the pseudomonas cellulolytica according to the inoculation amount of 10% volume fraction, and then uniformly mixing at the rotation speed of 150r/min at 55 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a complex microbial inoculum: weighing 60 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 20 parts by weight of the lactobacillus agent and 20 parts by weight of the bacillus subtilis agent according to the dry weight, and uniformly mixing to obtain the composite microbial agent.
(3) Preparing a digestion promoting biological feed: and (3) adding the compound microbial inoculum prepared in the step (2) into a basic feed according to the mass fraction of 15%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 4: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding the wheat straw and glucose culture medium pretreated by the combination of an alkaline method and a steam explosion method into a reaction device according to the solid-liquid weight-volume ratio of 1:40, inoculating clostridium thermocellum according to the inoculum size of 10% volume fraction, and then uniformly mixing at the rotating speed of 150r/min at 60 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a complex microbial inoculum: weighing 70 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 15 parts by weight of lactobacillus agent and 15 parts by weight of bacillus subtilis agent according to the dry weight, and uniformly mixing to obtain the composite microbial agent.
(3) Preparing a digestion promoting biological feed: and (3) adding the compound microbial inoculum prepared in the step (2) into a basic feed according to the mass fraction of 15%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 5: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding a straw and glucose culture medium pretreated by a sulfonation method and a hydrothermal method in a combined manner into a reaction device according to a solid-liquid weight-volume ratio of 1:50, inoculating clostridium thermocellum according to an inoculation amount of 10% volume fraction, and then uniformly mixing at a rotation speed of 150r/min at 60 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a complex microbial inoculum: weighing 80 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 15 parts by weight of lactobacillus agent and 5 parts by weight of bacillus subtilis agent according to the dry weight, and uniformly mixing to obtain the composite microbial agent.
(3) Preparing a digestion promoting biological feed: and (3) adding the compound microbial inoculum prepared in the step (2) into the basic feed according to the mass fraction of 20%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 6: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding the corn straw and the glucose culture medium subjected to sulfonation-hydrothermal combined pretreatment into a reaction device according to a solid-liquid weight-volume ratio of 1:20, inoculating clostridium thermocellum according to an inoculation amount of 5% volume fraction, and then uniformly mixing at a rotating speed of 100r/min at 60 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a digestion promoting biological feed: and (2) adding the cellulosome-producing microbial agent prepared in the step (1) into the basic feed according to the mass fraction of 7%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 7: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding corn straws subjected to sulfonation-hydrothermal combined pretreatment and a glucose culture medium into a reaction device according to a solid-liquid weight-volume ratio of 1:20, inoculating yellow clostridium cellulolyticum according to an inoculation amount of 5% volume fraction, and then uniformly mixing at a rotating speed of 100r/min at 65 ℃; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a digestion promoting biological feed: and (2) adding the cellulosome-producing microbial agent prepared in the step (1) into the basic feed according to the mass fraction of 30%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 8: preparation of digestion promoting biological feed
In contrast to the embodiment 1, the process of the invention,
(1) preparing a cellulosome-producing microbial agent: adding the corn straw and the glucose culture medium subjected to sulfonation-hydrothermal combined pretreatment into a reaction device according to a solid-liquid weight-volume ratio of 1:20, inoculating pseudomonas cellulolytica according to an inoculation amount of 5% volume fraction, and then uniformly mixing at 42 ℃ and a rotation speed of 100 r/min; checking the pressure in the container at regular time, deflating, and finishing the culture when no positive pressure exists in the container; centrifuging to separate solid from liquid, and obtaining a solid phase which is the microbial agent for producing the cellulosome.
(2) Preparing a digestion promoting biological feed: and (2) adding the cellulosome-producing microbial agent prepared in the step (1) into the basic feed according to the mass fraction of 30%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
Example 9: laying hen feeding test
300 Hailan brown laying hens which are 30 weeks old and have similar and healthy production performance are selected for the test and randomly divided into 6 groups, each group is divided into 5 groups, and each group is 10 chickens. Test groups 1-5 were fed with the lignocellulose-based biofeeds prepared in examples 1-5, respectively, and a blank control group, fed with basal diet only. Feeding 150g of feed for each chicken every day, feeding twice every day with free drinking water, and feeding for 30 days.
Example 10: broiler feeding test
300 white feather broilers with similar production performance at 30 days of age are selected for the test and randomly divided into 6 groups, each group is divided into 5 groups, and each group is 10 chickens. Test groups 1-5 were fed with the lignocellulose-based biofeeds prepared in examples 1-5, respectively, and a blank control group, fed with basal diet only. The feeding method comprises the following steps: in the first week, the food is taken freely and the water is drunk freely; turning off the lamp for 2-3 hours before, at noon and after dark every day, and turning on the lamp to feed or drink water freely in the rest of the day. Feeding for 30 days, weighing and recording the weight increasing condition of each broiler chicken, and calculating the feed-meat ratio.
TABLE 1 index parameters of the digestion-promoting biological feeds prepared in examples 1 to 5 after feeding the chickens
As can be seen from Table 1, in examples 1-5, the compound microbial inoculum is used for preparing the digestion promoting feed, and the incidence of diseases of laying hens and broilers fed with the digestion promoting feed is 0-2%, and is reduced by 2-4% compared with a control group, and even zero incidence of diseases is achieved; wherein, the laying rate of the laying hens is 92-96%, and compared with a control group, the laying rate is increased by 4-8 percentage points; the feed conversion ratio of the broiler chickens is 1.41-1.71, and compared with a control group, the feed conversion ratio is reduced by 10-25.8%.
Examples 6 to 8 adopt the digestion promoting feed prepared by the cellulosome-producing microbial agent, the incidence of the feed is 2% when the feed is used for feeding laying hens and broilers, and the incidence is slightly reduced compared with a control group; wherein, the laying rate of the laying hens is 90-92%, compared with a control group, the laying rate is increased by 2-4 percentage points, which is slightly lower than that of the laying hens in the examples 1-5; the feed-meat ratio of the broiler chickens is 1.68-1.74, and compared with a control group, the feed-meat ratio is reduced by 8.4-11.6%, and is also slightly lower than that of the broiler chickens in examples 1-5.
This shows that compared with the digestion promoting feed prepared by only using the cellulosome-producing microbial agent, the digestion promoting feed prepared by using the composite microbial agent has better effect. The method is characterized in that the lignocellulose-based soluble sugar obtained by degrading lignocellulose by using cellulosome-producing microorganisms is used for synergistically culturing probiotics such as bacillus subtilis, lactobacillus and the like, so that the digestion and absorption of food are further promoted, the laying rate is remarkably improved, and the feed conversion ratio is reduced; and the purposes of inhibiting the growth and reproduction of pathogenic bacteria and reducing the morbidity are achieved by using the cellulosome-producing microorganisms and lactic acid and acetic acid produced in the growth process of the probiotics.
Claims (8)
1. The digestion-promoting composite microbial inoculum for the biological feed is characterized in that: the composition comprises the following components in parts by weight: 40-80 parts of cellulosome-producing microbial agent, 15-37 parts of lactobacillus agent and 5-25 parts of bacillus subtilis agent, and uniformly mixing to obtain a composite microbial agent;
the cellulosome-producing microbial agent is prepared by the following method: adding pretreated lignocellulose raw material and glucose culture medium into a reaction device according to the solid-liquid weight-volume ratio of 1:20-1:50, inoculating the microorganism producing the cellulosome according to the inoculation amount of 5-10% volume fraction, then uniformly mixing at 42-65 ℃ and the rotation speed of 100-150r/min, checking the pressure in the container at regular time, and releasing gas to finish the culture when no positive pressure exists in the container; centrifuging to perform solid-liquid separation, wherein the obtained solid phase is a microbial agent for producing the cellulosome;
the cellulosome-producing microorganism is clostridium thermocellum, clostridium thermocellum yellow or pseudomonas fibrinolyticum.
2. The digestion promoting complex microbial inoculum for biological feed according to claim 1, which is characterized in that: the pretreatment is one or more of alkali method, hydrothermal method, steam explosion method and sulfonation method pretreatment technology.
3. The digestion promoting complex microbial inoculum for biological feed according to claim 1, which is characterized in that: the lignocellulose raw material is one or a combination of a plurality of corn stalks, wheat straws, corncobs, corn husks and rice straws.
4. The digestion promoting complex microbial inoculum for biological feed according to claim 1, which is characterized in that: the glucose culture medium is prepared by adding 2.9g/L dipotassium hydrogen phosphate, 1.5g/L potassium dihydrogen phosphate, 0.8g/L urea, 0.1g/L calcium chloride, 1.8g/L magnesium chloride, 0.0005g/L ferrous sulfate, 2g/L sodium sulfide, 4g/L corn steep liquor, 2g/L trisodium citrate and 1-20g/L, pH 6.5.5-7.5 glucose per liter of water.
5. The digestion promoting complex microbial inoculum for biological feeds according to any one of claims 1 to 4, which is characterized in that: the reaction device is an anaerobic fermentation device with a stirring paddle.
6. The use of the digestion promoting complex microbial inoculum for biological feeds according to any one of claims 1 to 4, characterized in that: the composite microbial inoculum is used for preparing digestion-promoting biological feed.
7. The preparation method of the digestion promoting biological feed is characterized by comprising the following steps: the method comprises the following steps:
(1) preparing a cellulosome-producing microbial agent: adding the pretreated lignocellulose raw material and a glucose culture medium into a reaction device according to the solid-liquid weight-volume ratio of 1:20-1:50, and then uniformly mixing at the rotation speed of 100-; checking the pressure in the container at regular time, and releasing the gas to finish the culture when no positive pressure exists in the container; centrifuging to perform solid-liquid separation, wherein the obtained solid phase is a microbial agent for producing the cellulosome;
(2) preparing a complex microbial inoculum: weighing 40-80 parts by weight of the cellulosome-producing microbial agent obtained in the step (1), 15-37 parts by weight of a lactobacillus agent and 5-25 parts by weight of a bacillus subtilis agent according to dry weight, and uniformly mixing to obtain a composite microbial agent; the cellulosome-producing microorganism is clostridium thermocellum, clostridium thermocellum yellow or pseudomonas fibrinolyticum;
(3) preparing a digestion promoting biological feed: and (3) adding the cellulosome-producing microbial agent prepared in the step (1) or the composite microbial agent prepared in the step (2) into the basic feed according to the mass fraction of 7-30%, and uniformly stirring to obtain the digestion-promoting reinforced biological feed.
8. The method of preparing a digestion promoting biological feed as set forth in claim 7, wherein: the basic chicken feed consists of the following components: 65 parts of corn, 20 parts of bean cake, 3 parts of fish meal, 1.8 parts of bone meal, 0.2 part of salt and 10 parts of green vegetable leaves.
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