CN109172734A - 中药组合物、制备方法及应用 - Google Patents
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Abstract
本发明公开了一种中药组合物，包括以下重量份数的原料：苦丁50份，女贞子20份，麦冬50份，车前草20份，川芎20份，野菊花80份。本发明还公开一种中药组合物的制备方法及应用。本发明不添加稳定剂、防腐剂，制备的产品具有较好的消炎、止咳效果，具有较大的推广价值。
Description
技术领域
本发明涉及医药领域。更具体地说，本发明涉及一种中药组合物、制备方法及应用。
背景技术
急性咽炎为咽部黏膜及黏膜下组织的急性炎症，咽部红肿疼痛，或干燥、异物感，咽痒不适等为主要表现，多由素体肺肾阴虚，或风热喉痹反复发作，余邪留滞不清，伤津耗液，使阴液亏损，咽喉失于濡养，兼之虚火上的，从而导致本病的发生。病变主要在黏膜层，表现为咽部黏膜慢性充血。黏膜及黏膜下结缔组织增生。黏液腺可肥大，分泌功能亢进，黏液分泌增多。急性咽炎容易引起咳嗽，目前并没有较好的针对两种病症的中药。
发明内容
本发明的一个目的是解决至少上述问题，并提供至少后面将说明的优点。
本发明还有一个目的是提供一种中药组合物、制备方法及应用，其不添加稳定剂、防腐剂，制备的产品具有较好的消炎、止咳效果，具有较大的推广价值。
为了实现根据本发明的这些目的和其它优点，提供了一种中药组合物，包括以下重量份数的原料：苦丁50份，女贞子20份，麦冬50份，车前草20份，川芎20份，野菊花80份。
中药组合物的制备方法，包括以下步骤：
步骤一、将上述重量份数的车前草加10倍量的水进行蒸馏，收集蒸馏液；
步骤二、将上述重量份数的苦丁、女贞子、麦冬、川芎加10倍量的水煎煮，煮沸后冷却至80℃，将上述重量份数的野菊花加入，冷烫1min，加热水提2次，每次1h，过滤，合并溶液得到水提液；
步骤三、将水提液减压浓缩至低于50℃、相对密度为1.2，加入乙醇使含醇量达到60％，过滤，减压回收乙醇，得浸膏；
步骤四、将浸膏加10倍量的水、0.2倍量的柠檬酸煎煮，冷却至室温，在0-4℃静置冷藏3d，加入0.005倍量的活性炭，煎煮，过滤，得清膏；
步骤五、向步骤三得到的清膏中加入步骤一的蒸馏液和水定容至800mL，加入苯甲酸钠，调节pH至7.0，加入甜味剂，定容至1000mL，过滤，灭菌，即得。
优选的是，步骤五中调节pH用20wt.％氢氧化钠溶液。
优选的是，步骤五中的甜味剂为45wt.％蔗糖、55wt.％甜菊糖苷复合糖浆。
中药组合物在制备治疗急性咽炎的药物的应用。
中药组合物在制备治疗咳嗽的药物的应用。
本发明至少包括以下有益效果：
本发明不添加稳定剂、防腐剂，产品具有良好的稳定性，本发明的制备方法工艺简单、利于生产，本发明制备的产品具有较好的消炎、止咳效果，具有较大的推广价值。
本发明的其它优点、目标和特征将部分通过下面的说明体现，部分还将通过对本发明的研究和实践而为本领域的技术人员所理解。
具体实施方式
下面结合实例对本发明做进一步的详细说明，以令本领域技术人员参照说明书文字能够据以实施。
应当理解，本文所使用的诸如“具有”、“包含”以及“包括”术语并不配出一个或多个其它元件或其组合的存在或添加。
需要说明的是，下述实施方案中所述实验方法，如无特殊说明，均为常规方法，所述试剂和材料，如无特殊说明，均可从商业途径获得。
<实例1>
中药组合物，包括以下重量份数的原料：苦丁50份，女贞子20份，麦冬50份，车前草20份，川芎20份，野菊花80份。
制备方法包括以下步骤：
步骤一、将上述重量份数的车前草加10倍量的水进行蒸馏，收集蒸馏液；
步骤二、将上述重量份数的苦丁、女贞子、麦冬、川芎加10倍量的水煎煮，煮沸后冷却至80℃，将上述重量份数的野菊花加入，冷烫1min，加热水提2次，每次1h，过滤，合并溶液得到水提液；
步骤三、将水提液减压浓缩至低于50℃、相对密度为1.2，加入乙醇使含醇量达到60％，过滤，减压回收乙醇，得浸膏；
步骤四、将浸膏加10倍量的水、0.2倍量的柠檬酸煎煮，冷却至室温，在0-4℃静置冷藏3d，加入0.005倍量的活性炭，煎煮，过滤，得清膏；
步骤五、向步骤三得到的清膏中加入步骤一的蒸馏液和水定容至800mL，加入苯甲酸钠，用20wt.％氢氧化钠溶液调节pH至7.0，加入45wt.％蔗糖、55wt.％甜菊糖苷复合糖浆，定容至1000mL，过滤，灭菌，即得。
<治疗急性咽炎试验>
2.1试验动物
SD大鼠，SPF级，体重120g，60只，雌雄各半。
1.2方法
1.2.1动物分组
SD大鼠随机分为4组，每组雌雄各半，分别为阿莫西林组、空白组、模型组、给药组。
1.2.2急性咽炎模型的构造
阿莫西林组、模型组、给药组，每天向大鼠咽喉部喷浓度为15vol.％的氨水，每天上午、下午各1次，空白组每天喷等体积生理盐水，连续7d，每日观察大鼠状态、咽喉情况，包括黏膜形态、色泽等。各组大鼠d8灌胃药物，阿莫西林组为阿莫西林肠溶片，按照0.4g生药/kg的剂量，空白组、模型组为生理盐水，给药组为实例1制备的药物，按照0.4g生药/kg的剂量，每天1次，连续7d。
1.3试验结果
1.3.1大鼠行为学观察
喷氨水后，模型组、阿莫西林组和给药组的大多数的大鼠出现搔抓口部，口腔分泌物增多，饮水量增多，给药7d后，阿莫西林组和给药组的症状得到不同程度的减轻，模型组的大鼠咽部充血，呈暗红色，肿胀。
1.3.2血常规检测结果
如表1所示，与空白组相比，模型组的单核细胞、中性粒细胞的百分比含量增加，淋巴细胞百分比含量下降，嗜酸性粒细胞百分比含量无明显变化。阿莫西林组和给药组的单核细胞及中性粒细胞百分比含量减少，淋巴细胞百分比含量增加，嗜酸性粒细胞百分比含量无明显变化。
表1
1.3.3咽喉组织IL-6的表达水平
如表2所示，喷氨水后，模型组、阿莫西林组和给药组的大鼠的粘膜组织中IL-6表达水平较空白组的大鼠显著升高，阿司匹林组和给呀铺租能显著降低IL-6含量。
表2
1.3.4咽喉病理组织学观察
空白组大鼠咽喉黏膜表被复层鳞状上皮，上皮下薄层结缔组织，可见黏液腺或混合腺，黏膜上皮细胞无坏死，上皮内核上皮下无炎细胞浸润，病变评分为0。
模型组大鼠多数咽喉壁组织充血，咽喉黏膜上皮细胞轻度或中度变性、坏死，上皮内或咽壁有中度言必报浸润，炎细胞以中性粒细胞为主，病变评分为4.25±2.15。
阿司匹林组和给药组能使大鼠咽喉黏膜病变程度明显减轻，阿司匹林组的病变评分为0.96±0.62，给药组的病变评分为1.21±0.34。
<治疗咳嗽试验>
2.1试验动物
SD大鼠，SPF级，体重120g，30只，雌雄各半。
2.2试验方法
将全部SD大鼠置于观察室内，喷乳17.5wt.％的枸橼酸，喷雾1min，观察记录大鼠在5min之内的咳嗽潜伏期和咳嗽次数，将咳嗽潜伏期≤10s或≥120s，以及5min内的咳嗽次数≤10次或≥50次的大鼠全部剔除，剩下的大鼠为预选合格的大鼠，随机分为两组，即模型组和给药组。两组连续灌胃给药7d，每天1次，给药组按照3g生药/kg的剂量，模型组以等体积蒸馏水。末次给药1h后，观察记录大鼠咳嗽潜伏期及5min内的咳嗽次数。
2.3试验结果
如表3所示，与模型组相比，给药组的大鼠咳嗽潜伏期(P＜0.05，P＜0.01)长，咳嗽次数(P＜0.001)少。
表3
这里说明的设备数量和处理规模是用来简化本发明的说明的。对本发明的应用、修改和变化对本领域的技术人员来说是显而易见的。
尽管本发明的实施方案已公开如上，但其并不仅仅限于说明书和实施方式中所列运用，它完全可以被适用于各种适合本发明的领域，对于熟悉本领域的人员而言，可容易地实现另外的修改，因此在不背离权利要求及等同范围所限定的一般概念下，本发明并不限于特定的细节和这里示出与描述的实例。
Claims (6)
1.中药组合物，其特征在于，包括以下重量份数的原料：苦丁50份，女贞子20份，麦冬50份，车前草20份，川芎20份，野菊花80份。
2.如权利要求1所述的中药组合物的制备方法，其特征在于，包括以下步骤：
步骤一、将上述重量份数的车前草加10倍量的水进行蒸馏，收集蒸馏液；
步骤二、将上述重量份数的苦丁、女贞子、麦冬、川芎加10倍量的水煎煮，煮沸后冷却至80℃，将上述重量份数的野菊花加入，冷烫1min，加热水提2次，每次1h，过滤，合并溶液得到水提液；
步骤三、将水提液减压浓缩至低于50℃、相对密度为1.2，加入乙醇使含醇量达到60％，过滤，减压回收乙醇，得浸膏；
步骤四、将浸膏加10倍量的水、0.2倍量的柠檬酸煎煮，冷却至室温，在0-4℃静置冷藏3d，加入0.005倍量的活性炭，煎煮，过滤，得清膏；
步骤五、向步骤三得到的清膏中加入步骤一的蒸馏液和水定容至800mL，加入苯甲酸钠，调节pH至7.0，加入甜味剂，定容至1000mL，过滤，灭菌，即得。
3.如权利要求2所述的中药组合物的制备方法，其特征在于，步骤五中调节pH用20wt.％氢氧化钠溶液。
4.如权利要求2所述的中药组合物的制备方法，其特征在于，步骤五中的甜味剂为45wt.％蔗糖、55wt.％甜菊糖苷复合糖浆。
5.如权利要求1所述的中药组合物在制备治疗急性咽炎的药物的应用。
6.如权利要求1所述的中药组合物在制备治疗咳嗽的药物的应用。
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