CN109169413A - A kind of separation method and transplantation method of the single anthocyathus of helmet shape coral - Google Patents

A kind of separation method and transplantation method of the single anthocyathus of helmet shape coral Download PDF

Info

Publication number
CN109169413A
CN109169413A CN201811264973.0A CN201811264973A CN109169413A CN 109169413 A CN109169413 A CN 109169413A CN 201811264973 A CN201811264973 A CN 201811264973A CN 109169413 A CN109169413 A CN 109169413A
Authority
CN
China
Prior art keywords
anthocyathus
coral
branch
helmet shape
corallite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201811264973.0A
Other languages
Chinese (zh)
Other versions
CN109169413B (en
Inventor
张浴阳
黄晖
江雷
袁翔城
雷新明
袁涛
练健生
杨剑辉
刘胜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China Sea Institute of Oceanology of CAS
Original Assignee
South China Sea Institute of Oceanology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China Sea Institute of Oceanology of CAS filed Critical South China Sea Institute of Oceanology of CAS
Priority to CN201811264973.0A priority Critical patent/CN109169413B/en
Publication of CN109169413A publication Critical patent/CN109169413A/en
Application granted granted Critical
Publication of CN109169413B publication Critical patent/CN109169413B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention provides the separation methods and transplantation method of a kind of single anthocyathus of helmet shape coral, belong to the artificial recovery technique field of coral reef ecology.Separation method includes: that the mature place for developing helmet shape corallite to be separated from the anthocyathus of center is dug into two, it is initially separated in the incision position chiseled: entangling the head of any anthocyathus using tubular apparatus, it pushes to destroy anthocyathus with the coenosteum between the anthocyathus of periphery, then breaks out the anthocyathus entangled by tubular apparatus from helmet shape corallite.The separation method is simple, can play the role of reducing coral parent demand, reduce to destroy field coral and provide and more cultivate monomer.Transplantation method includes: that above-mentioned isolated anthocyathus is fixed in the transplanting hole of transplantation device, transplantation device is inserted into the coral reef in transplanting region.The transplantation method is simple, greatly alleviates the demand to coral parent, and can resist moderate sea wave disturbance, avoiding device roll and topple over push down coral cause its death.

Description

A kind of separation method and transplantation method of the single anthocyathus of helmet shape coral
Technical field
The invention belongs to coral reef ecology manually recovery technique fields, and in particular to a kind of single anthocyathus of helmet shape coral Separation method and transplantation method.
Background technique
Important feature biology of the project cost consulting item as coral reef, has been put into " endangered species of wild fauna and flora kind state at present Border trade pact " in annex two, as important protection animal by transaction control and protection.Due to by mankind's activity, complete Ball climate change, harmful organisms, marine pollution etc. threaten, and the project cost consulting item quantity on coral reef is falling sharply.Investigation at present The display whole world is more than that seriously degenerating occurs in the coral reef of half, and degenerative process is also accelerating, and scientist predicts according to current Degradation trend, may disappear to end of this century coral reef ecologic system.Therefore, various countries to the protection of project cost consulting item further Pay attention to, destructive activity and coral trading activity of the strict control to project cost consulting item, and the training of Efforts To Develop project cost consulting item It educates technical research and promotes.
Helmet shape coral belongs to as one important in project cost consulting item, widely distributed in world's coral reef, from Red sea, east The seas of South Asia to Great Barrier Reef be Common Species.In China from Fujian, Guangdong, Guangxi, Hainan bank to the Xisha Islands, the Nansha Islands Also there is helmet shape coral growth.Since tolerance of the helmet shape coral to adverse circumstances is strong, especially deposit and pollutant compared with In more environment, such as Xuwen, Daya Gulf, Sanya sea area, one of helmet shape coral or the sociales of coral reef.Since it is resistance to By this strong feature of property, one of the important coral type under research coastal pollution environment is become, while being directed to helmet shape coral The protection of coral is also gradually being reinforced with work is cultivated.
Since the protection for project cost consulting item is increasingly stringenter, coral laboratory sample is obtained from field and for cultivating coral The parent of coral also requires a small amount of as far as possible, influence and destruction of the reduction to field coral.Therefore, how to be injured using the smallest to coral Coral is sampled and is cultivated as the experiment of current coral and problem to be solved in coral cultivation.
Summary of the invention
One of the objects of the present invention is to provide a kind of separation method of single anthocyathus of helmet shape coral, separation method letters It is single, it can be used for the artificial culture of project cost consulting item experiment and helmet shape coral, to play the coral parent demand that reduces, reduce to field Coral destroys and provides more effects for cultivating monomer.
Another object of the present invention is to provide a kind of transplantation methods of single anthocyathus of helmet shape coral, and this method is simple, The demand to coral parent can greatly be mitigated, and moderate sea wave disturbance can be resisted, though device generate rolling and Topple over, coral will not be pushed down completely by device, cause death.
The present invention solves its technical problem and adopts the following technical solutions to realize:
The embodiment of the present invention proposes a kind of separation method of single anthocyathus of helmet shape coral comprising following steps:
The mature place that helmet shape corallite to be separated is developed from the anthocyathus of center is dug into two, in the fracture chiseled Place is initially separated: being entangled the head of any anthocyathus using tubular apparatus, is pushed to destroy anthocyathus between the anthocyathus of periphery Then coenosteum breaks out the anthocyathus entangled by tubular apparatus from helmet shape corallite.
In a preferred embodiment of the invention, helmet shape corallite to be separated is through including the following steps to obtain: The helmet shape corallite of field health is acquired, the precuring in breeding environment is with health helmet shape corallite resulting after precuring Helmet shape corallite to be separated.
Wherein, breeding environment includes: that the temperature of breeding water body and the temperature collection of helmet shape corallite differ and be no more than 2 DEG C, and/or cultivation intensity of illumination is 50-200 μm of olm-2·s-1, and/or cultivation salinity is 28-34.
In a preferred embodiment of the invention, the living tissue area of the helmet shape corallite of the field health of acquisition is not Lower than 30cm2
In a preferred embodiment of the invention, the big 1- of diameter of the internal diameter of tubular apparatus compared with anthocyathus to be entangled 3mm。
In a preferred embodiment of the invention, the pipe thickness of tubular apparatus is not more than 0.5mm.
The embodiment of the present invention also proposed a kind of transplantation method of single anthocyathus of helmet shape coral comprising following steps: It will be fixed in the transplanting hole of transplantation device by the isolated anthocyathus of above-mentioned separation method, by transplantation device by being fixed with coral The mode that one end of coral cup is upward is inserted into the coral reef in transplanting region.
Transplantation device includes interconnecting piece and at least one branch, and one end of each branch is all connected to interconnecting piece, at least one The end of one end of the separate interconnecting piece of branch offers the transplanting hole for fixing anthocyathus.
In a preferred embodiment of the invention, transplantation device includes that transplantation device includes interconnecting piece, the first branch, Two branches, third branch and the 4th branch, the second branch, third branch and the 4th branch are from interconnecting piece to far from the first branch Direction extend and form tripod-shaped to be fixed in coral reef as fixed feet, one end of the separate interconnecting piece of the first branch Offer transplanting hole and for fixing anthocyathus.
In a preferred embodiment of the invention, the length of each branch is 3-10cm.
In a preferred embodiment of the invention, the diameter for transplanting hole is 5-8mm, and depth is not less than 1cm.
In a preferred embodiment of the invention, when it is to carry out in air that anthocyathus, which is fixed in transplanting hole, Gu Surely it operates in 20min and completes, then the transplantation device for being fixed with anthocyathus is put into seawater.
The separation method of the single anthocyathus of helmet shape coral and the beneficial effect of transplantation method include: in the embodiment of the present invention
The separation method of the single anthocyathus of helmet shape coral provided in an embodiment of the present invention is easy to operate, can be used for making reef coral The artificial culture of coral experiment and helmet shape coral can play the coral parent demand that reduces, reduce and destroy and provide to field coral More effects for cultivating monomer.
The transplantation method of the single anthocyathus of helmet shape coral can greatly mitigate the demand to coral parent, and can resist Etc. degree sea wave disturbance, even if device generate rolling and topple over, coral will not be pushed down completely by device, cause death.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this A little attached drawings obtain other relevant attached drawings.
Fig. 1 is that there are four the structural schematic diagrams of the transplantation device of branch for tool provided by the embodiments of the present application.
Icon: 10- transplantation device;11- interconnecting piece;15- branch;The first branch of 151-;The second branch of 152-;153- third Branch;The 4th branch of 154-;20- transplants hole.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects It encloses.
In the description of the present invention, it should be noted that the orientation or position of the instructions such as term " on ", "lower", "inner", "outside" Set relationship be based on the orientation or positional relationship shown in the drawings or the invention product using when the orientation or position usually put Relationship is set, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning are necessary It with specific orientation, is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second " etc. are only used for distinguishing description, are not understood to indicate or imply relative importance.
In addition, the terms such as term " vertical " are not offered as requiring component absolute upright, but can be slightly tilted.As " hung down Directly " only refer to that its direction is more vertical with respect to for "horizontal", be not indicate the structure have to it is completely vertical, but can To be slightly tilted.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ", " installation ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally connect It connects;It can be mechanical connection, be also possible to be electrically connected;It can be directly connected, can also indirectly connected through an intermediary, it can To be the connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood with concrete condition Concrete meaning in the present invention.
It is specifically described with reference to embodiments.
Embodiment
This application involves the separation method of the single anthocyathus of helmet shape coral may comprise steps of: by helmet to be separated Chisel into two, is initially separated in the incision position chiseled from the maturation that shape corallite is developed from the anthocyathus of center: using tubulose Utensil (such as stainless steel tube, hereinafter referred to as steel pipe) entangles the head of any anthocyathus, pushes to destroy anthocyathus with periphery coral Coenosteum between cup, then breaks out the anthocyathus entangled by tubular apparatus from helmet shape corallite.
Wherein, helmet shape corallite to be separated can be through including the following steps to obtain: the helmet shape coral of acquisition field health Coral individual, the precuring in breeding environment, with health helmet shape corallite resulting after precuring for helmet shape corallite to be separated.
In this application, " health " can with but be not limited only to not occur albefaction, helminth or hydranth on corallite The lesions such as atrophy and the symptom of portion of tissue death as judgment criteria, may also include it is well known in the art itself it is judged that mark It is quasi-.
In this application, the living tissue area of the helmet shape corallite of the field health of acquisition is not less than 30cm2, to avoid Anthocyathus individual after separation is smaller and is unfavorable for surviving.Specifically, above-mentioned or tissue area can be 30cm2、32cm2、 34cm2、35cm2、36cm2、38cm2Or 40cm2Deng.
Optionally, the temperature of breeding water body differs with the temperature collection of helmet shape corallite and is no more than in breeding environment 2 DEG C, preferably more than 1 DEG C cause albefaction, disease stress and occur to avoid the too big adaptability for reducing coral of the two temperature difference Phenomena such as disease and death.Breeding water body can be running water or recirculated water, and preferably (running water refers in breeding process running water In, constantly there is new water body to flow into, while old water body outflow).
Optionally, intensity of illumination being cultivated in breeding environment can be 50-200 μm of olm-2·s-1, such as 50 μ·mol·m-2·s-1、60μ·mol·m-2·s-1、80μ·mol·m-2·s-1、100μ·mol·m-2·s-1、120μ· mol·m-2·s-1、140μ·mol·m-2·s-1、160μ·mol·m-2·s-1、180μ·mol·m-2·s-1Or 200 μ mol·m-2·s-1Deng.Above-mentioned intensity of illumination range is well suited to the survival of helmet shape coral, and intensity of illumination is excessively high to be easy to cause coral white Change;The too low photosynthesis that can reduce coral parachorium algae of intensity of illumination causes it can not the enough energy matter confessions of output To coral, cause coral growth slowly or body decline, atrophy even death.
Optionally, in breeding environment salinity can be 28-34, such as 28,28.5,29,29.5,30,30.5,31, 31.5,32,32.5,33,33.5 or 34 etc..Above-mentioned salinity refers to the ratio of dissolution material mass and water quality.Salinity control is existed In above range, it can effectively avoid the increase of cell wall osmotic pressure from rupturing, lose original function.
The time of precuring can be 7-15 days, such as 7 days, 8 days, 10 days, 12 days, 14 days or 15 days, by above-mentioned item Precuring under part can be conducive to coral and adapt to new breeding environment, the single coral separated caused by the unlikely acute variation because of environment Cup is dead.
Observe whether corallite such as albefaction, lesion or the unsound presentation of hydranth atrophy occurs after precuring, with pre- Resulting healthy helmet shape corallite is helmet shape corallite to be separated after supporting, and hydranth is presented in above-mentioned health shape corallite The health status such as tentacle expansion and coral color are normal.It is worth noting that there is unsound shape in coral after precuring Condition can adjust cultivating condition in time, until coral is got well.
Before helmet shape corallite is dug into two, it can carry out disinfection to helmet shape corallite, it is that it contains or latent to remove In germ that may be present and helminth etc..
In specific operation process, it can be used helmet shape corallite to be separated such as hammer and flat mouth chisel from the coral of center (preferably most mature place) chisel into two, then from the incision position chiseled is separated at the maturation of coral cup development.
Preferably, in the present embodiment, tubular apparatus is hollow tubulose, and material is not particularly limited.The tubular apparatus Internal diameter compared with the big 1-3mm of diameter of anthocyathus to be entangled, on the one hand can be convenient for head that completely anthocyathus is exposed It entangles, and the anthocyathus head that will not damage or rub;On the other hand certain fixed function can be played to above-mentioned anthocyathus, with Anthocyathus during subsequent operation is avoided to fall off from tubular apparatus.
By taking common anthocyathus as an example, the internal diameter of tubular apparatus accordingly can be 6-10mm, as 6mm, 6.5mm, 7mm, 7.5mm, 8mm, 8.5mm, 9mm, 9.5mm or 10mm etc..In addition, the pipe thickness of tubular apparatus is preferably no greater than 0.5mm, such as 0.5mm, 0.45mm, 0.4mm, 0.35mm or 0.3mm etc..
It is vertical firmly to push 1-2cm after tubular apparatus entangles the head of any anthocyathus of incision position, to destroy anthocyathus It with the coenosteum between its periphery anthocyathus, then gently moves, can be broken out from helmet shape corallite by tubulose device outward again Has the anthocyathus entangled.
The anthocyathus separated can be by operating the extraction from tubular apparatus (such as steel pipe) as follows: pinching and is exposed at tubulose device Coral bone portion outside tool is gently extracted out from tubular apparatus, and is immediately placed in and is cultivated in seawater, and preparatory standard is vertically fixed on In the pedestal got ready, avoid traverse or stand upside down to place.By 7 days temporarily support, such as without death, Telatrophy, color change and divide Phenomena such as secreting mucus, which can be used for coral experiment or coral is cultivated.
Directly it can also use the separating tools such as tweezers or nipper plier will be single it is worth noting that separating anthocyathus in the application A anthocyathus is under parent coral sled, and it is preferable to employ tubular apparatus.It is avoided as far as possible when sled takes anthocyathus on injury anthocyathus Coral tissue, and keep the cylindrical morphology of anthocyathus.Isolated single anthocyathus is placed in seawater in time to be guaranteed It is survived, and further takes out when needing and using or transplant.
Further, when needing to obtain multiple anthocyathuses, i.e., with separating tool in the incision position chiseled according to above-mentioned point From method repeated isolation.
It holds, it is identical to obtain hundreds of genotype from single coral parent using the method that above-mentioned one cup separates Anthocyathus, since their genotype is identical, the varying environment that can be used in coral experiment influences the comparison acted on coral Experiment.Also, the above method can also from different coral parents isolated different genotype anthocyathus, be used for coral The comparative experiments that different corals are influenced under identical environment in experiment.Meanwhile the anthocyathus being isolated through above-mentioned separation method All have the ability for being grown to serve as new corallite, the demand in coral cultivation to field coral can be effectively reduced, protected indirectly The quantity of field coral is protected.
It further, may include following present invention also provides a kind of transplantation method of single anthocyathus of helmet shape coral Step: the shifting of transplantation device 10 will be fixed on by the isolated anthocyathus of the separation method of the above-mentioned single anthocyathus of helmet shape coral It plants in hole 20, transplantation device 10 is inserted into the coral reef in transplanting region by the upward mode in the one end for being fixed with anthocyathus.
Specifically, anthocyathus can be fixed in the transplanting hole 20 of transplantation device 10 (such as first by single coral by adhesive It in the transplanting hole 20 of coral cup insertion transplantation device 10, is then fixed again with adhesive).Wherein, adhesive is suitable for water environment With glue, the gelatin substance in water also can be used to, such as may include underwater epoxy resin glue or underwater gel glue.
Transplanting can carry out in seawater or air, excellent when it is to carry out in air that anthocyathus, which is fixed in transplanting hole 20, Choosing completes fixed operation control in 20min, is put into seawater with the transplantation device 10 that will be fixed with anthocyathus, to reduce Its aerial exposure duration, after avoiding air themperature from being apparently higher than ocean temperature, anthocyathus is easy in being transferred to seawater Generate stress and cause death.
In this application, transplantation device 10 includes interconnecting piece 11 and at least one branch 15, and one end of each branch 15 connects It is connected to interconnecting piece 11, one end of the separate interconnecting piece 11 of at least one branch 15 offers the transplanting hole 20 for fixing anthocyathus.
Optionally, the quantity of branch 15 can be 1,2,3 or 4 etc..When 15 quantity of branch be only 1 or When 15 quantity of person's branch is that multiple and multiple branches 15 extend from interconnecting piece 11 to the top of interconnecting piece 11, interconnecting piece 11 It is fixed on reef bottom with band or is pushed down with stone, to play fixed function.
When 15 quantity of branch be it is multiple when, can also be wherein at least 1 branch 15 do not set transplanting hole 20 and as fixation Foot is fixed in coral reef, remaining branch 15 then extends from interconnecting piece 11 to the top of interconnecting piece 11 as transplanting foot and at its end Portion opens up transplanting hole 20 namely fixed feet and transplanting foot is respectively facing the extension of two sides up and down of interconnecting piece 11.
As reference, transplantation device 10 includes interconnecting piece 11, the first branch 151, the second branch 152, third branch 153 With the 4th branch 154 (please referring to Fig. 1), the second branch 152, third branch 153 and the 4th branch 154 are from interconnecting piece 11 to remote Direction (namely lower section of interconnecting piece 11) from the first branch 151 extends and forms tripod-shaped to be fixed on coral as fixed feet In coral reef, the first branch 151 extends from interconnecting piece 11 to the top of interconnecting piece 11 and opens up in its one end far from interconnecting piece 11 For fixing the transplanting hole 20 of anthocyathus.It avoids being placed on husky bottom and silt as far as possible when it is worth noting that placing transplantation device 10 On mud, places depth and can be controlled in 2-15m.
Can refer to ground, the length of each branch 15 of above-mentioned transplantation device 10 all can be 3-10cm, as 3cm, 4cm, 5cm, 6cm, 7cm, 8cm, 9cm or 10cm can also be combined within the scope of 3-10cm.
Can refer to ground, the diameter in above-mentioned transplanting hole 20 can be 5-8mm, as 5cm, 5.5cm, 6cm, 6.5cm, 7cm, 7.5cm or 8cm, aperture depth are not less than 1cm.
It holds, by being fixed on separating obtained anthocyathus in the transplanting hole 20 of transplantation device 10, then by transplantation device 10 are integrally placed at transplanting region, can resist moderate sea wave disturbance, even if device generates rolling and topples over, coral is not yet It can be pushed down completely, cause death by device.
Test example
Coral transplanting is carried out in Sanya The Deer Turning lts Head coral reef, using point of the single anthocyathus of helmet shape coral provided by the present application It is fixed on transplantation device from acquiring helmet shape corallite with transplantation method and being decomposed into single anthocyathus, decomposes the single of acquisition Helmet shape 15 days survival rates of anthocyathus are 92%, and the single anthocyathus of survival in order, can be used for testing and cultivate with coral.It moves Same amount of corallite is planted, is only the 3% of conventional transplant quantity demand to coral parent quantity demand.Coral passes through 3 Moon growth, single anthocyathus proliferation are 12-34 anthocyathus, and growth rate is 15 times or more of conventional method.Transplanting survival rate is 95%, the survival rate compared to the single helmet shape anthocyathus 83% for being implanted in tiled surface improves about 12%.
To sum up, the separation method of the single anthocyathus of helmet shape coral provided in an embodiment of the present invention is easy to operate, can be used for making The artificial culture of reef coral experiment and helmet shape coral can play the coral parent demand that reduces, reduce and destroy to field coral And provide more effects for cultivating monomer.The transplantation method of the single anthocyathus of helmet shape coral can greatly mitigate to coral parent Demand, and moderate sea wave disturbance can be resisted, even if device generates rolling and topples over, coral will not be complete by device It pushes down, causes death.
These are only the preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification, Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (10)

1. a kind of separation method of the single anthocyathus of helmet shape coral, which comprises the following steps:
Chisel into two, is opened in the incision position chiseled from the maturation that helmet shape corallite to be separated is developed from the anthocyathus of center Begin to separate: entangling the head of any anthocyathus using tubular apparatus, pushes to destroy the anthocyathus between the anthocyathus of periphery Then coenosteum breaks out the anthocyathus entangled by the tubular apparatus from the helmet shape corallite.
2. separation method according to claim 1, which is characterized in that the helmet shape corallite to be separated included with Under step obtain: the helmet shape corallite of acquisition field health, the precuring in breeding environment, with healthy helmet resulting after precuring Shape corallite is the helmet shape corallite to be separated;
Wherein, breeding environment includes: that the temperature of breeding water body and the temperature collection of the helmet shape corallite differ and be no more than 2 DEG C, and/or cultivation intensity of illumination is 50-200 μm of olm-2·s-1, and/or cultivation salinity is 28-34.
3. separation method according to claim 2, which is characterized in that the helmet shape corallite of the field health of acquisition Living tissue area be not less than 30cm2
4. separation method according to claim 1, which is characterized in that the internal diameter of the tubular apparatus is compared with described in be entangled The big 1-3mm of the diameter of anthocyathus.
5. separation method according to claim 1, which is characterized in that the pipe thickness of the tubular apparatus is not more than 0.5mm。
6. a kind of transplantation method of the single anthocyathus of helmet shape coral, which comprises the following steps: will be by such as claim The isolated anthocyathus of the described in any item separation methods of 1-5 is fixed in the transplanting hole of transplantation device, by the shifting Planting apparatus is inserted into the coral reef in transplanting region by the upward mode in the one end for being fixed with the anthocyathus;
The transplantation device includes interconnecting piece and at least one branch, and one end of each branch is all connected to the interconnecting piece, Wherein the end of one end far from the interconnecting piece of 1 branch offers the transplanting for fixing the anthocyathus Hole.
7. transplantation method according to claim 6, which is characterized in that the transplantation device include interconnecting piece, the first branch, Second branch, third branch and the 4th branch, second branch, the third branch and the 4th branch are by the company Socket part extends to the direction far from first branch and forms tripod-shaped to be fixed in coral reef as fixed feet, described One end far from the interconnecting piece of first branch offers the transplanting hole and for fixing the anthocyathus.
8. transplantation method according to claim 7, which is characterized in that the length of each branch is 3-10cm.
9. transplantation method according to claim 6, which is characterized in that the diameter in the transplanting hole is 5-8mm, and depth is not low In 1cm.
10. transplantation method according to claim 6, which is characterized in that when the anthocyathus is fixed in the transplanting hole It is then fixed operation puts the transplantation device for being fixed with the anthocyathus in completing in 20min when carrying out in air Enter in seawater.
CN201811264973.0A 2018-10-26 2018-10-26 Separation method and transplantation method of single coral cup of helmet-shaped coral Active CN109169413B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811264973.0A CN109169413B (en) 2018-10-26 2018-10-26 Separation method and transplantation method of single coral cup of helmet-shaped coral

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811264973.0A CN109169413B (en) 2018-10-26 2018-10-26 Separation method and transplantation method of single coral cup of helmet-shaped coral

Publications (2)

Publication Number Publication Date
CN109169413A true CN109169413A (en) 2019-01-11
CN109169413B CN109169413B (en) 2020-11-27

Family

ID=64943930

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811264973.0A Active CN109169413B (en) 2018-10-26 2018-10-26 Separation method and transplantation method of single coral cup of helmet-shaped coral

Country Status (1)

Country Link
CN (1) CN109169413B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110999824A (en) * 2019-11-19 2020-04-14 东南大学 Fixing device and fixing method for universal transplanted coral
CN115024260A (en) * 2022-07-06 2022-09-09 中国科学院南海海洋研究所 Separable coral transplantation reef body

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08112048A (en) * 1994-10-18 1996-05-07 Oni Kobo:Kk Coral reef and its creation
JP2003009713A (en) * 2001-06-28 2003-01-14 Cp Farm:Kk Method of transplanting coral and unit for acclimatizing the same
JP2004147514A (en) * 2002-10-28 2004-05-27 Minoru Yuki Hook rest for dozuki terminal tackle and rod holder attached thereto
DE202005012158U1 (en) * 2005-07-29 2005-11-17 Goral, Christoph-Martin Modular system for culturing, breeding stone, soft and leather corals and mussels has transport element, intermediate holding element with round head and central recess, thread to connect foot, head and transport element, has cover and pipe
CN101258826A (en) * 2008-03-13 2008-09-10 哈尔滨工程大学 Frame work for assuring tree vertical growth at transplant early
CN101297640A (en) * 2008-06-23 2008-11-05 中国科学院南海海洋研究所 Ex situ conservation method of coral stone
CN101548661A (en) * 2009-04-30 2009-10-07 陈宏� Composite transplantation method of coral
CN201967503U (en) * 2011-02-25 2011-09-14 南京大学连云港高新技术研究院 Manual reef body
CN202153943U (en) * 2011-07-18 2012-03-07 深圳旭联海洋生物养殖有限公司 Quick connector of artificial reef coral
CN103444464A (en) * 2012-05-30 2013-12-18 张�浩 Full-crown seedling cultivation device
CN203968948U (en) * 2014-07-04 2014-12-03 海南大学 Seabed drift sand environment coral is transplanted reef base
CN105706895A (en) * 2016-03-10 2016-06-29 中国水产科学研究院南海水产研究所 Method for repairing intertidal sargasso field
CN106962251A (en) * 2017-04-28 2017-07-21 青岛越洋水产科技有限公司 A kind of trident branch shape fish shelter
CN206453005U (en) * 2017-02-22 2017-09-01 重庆大学 Assembled can adjust coral transplanting with trapping the dual-purpose reef base of the shoal of fish
CN108156993A (en) * 2018-01-31 2018-06-15 温州科技职业学院 A kind of special grafting tool of easy dragon fruit and its quick engrafting method

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08112048A (en) * 1994-10-18 1996-05-07 Oni Kobo:Kk Coral reef and its creation
JP2003009713A (en) * 2001-06-28 2003-01-14 Cp Farm:Kk Method of transplanting coral and unit for acclimatizing the same
JP2004147514A (en) * 2002-10-28 2004-05-27 Minoru Yuki Hook rest for dozuki terminal tackle and rod holder attached thereto
DE202005012158U1 (en) * 2005-07-29 2005-11-17 Goral, Christoph-Martin Modular system for culturing, breeding stone, soft and leather corals and mussels has transport element, intermediate holding element with round head and central recess, thread to connect foot, head and transport element, has cover and pipe
CN101258826A (en) * 2008-03-13 2008-09-10 哈尔滨工程大学 Frame work for assuring tree vertical growth at transplant early
CN101297640A (en) * 2008-06-23 2008-11-05 中国科学院南海海洋研究所 Ex situ conservation method of coral stone
CN101548661A (en) * 2009-04-30 2009-10-07 陈宏� Composite transplantation method of coral
CN201967503U (en) * 2011-02-25 2011-09-14 南京大学连云港高新技术研究院 Manual reef body
CN202153943U (en) * 2011-07-18 2012-03-07 深圳旭联海洋生物养殖有限公司 Quick connector of artificial reef coral
CN103444464A (en) * 2012-05-30 2013-12-18 张�浩 Full-crown seedling cultivation device
CN203968948U (en) * 2014-07-04 2014-12-03 海南大学 Seabed drift sand environment coral is transplanted reef base
CN105706895A (en) * 2016-03-10 2016-06-29 中国水产科学研究院南海水产研究所 Method for repairing intertidal sargasso field
CN206453005U (en) * 2017-02-22 2017-09-01 重庆大学 Assembled can adjust coral transplanting with trapping the dual-purpose reef base of the shoal of fish
CN106962251A (en) * 2017-04-28 2017-07-21 青岛越洋水产科技有限公司 A kind of trident branch shape fish shelter
CN108156993A (en) * 2018-01-31 2018-06-15 温州科技职业学院 A kind of special grafting tool of easy dragon fruit and its quick engrafting method

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
华北农业科学研究编译委员会等: "《植物的无性杂交》", 30 November 1951, 中华书局股份有限公司 *
周永灿等: "《海南省潜在海水增养殖区研究》", 30 November 2013, 海洋出版社 *
张浴阳等: ""西沙群岛珊瑚幼体培育实验",张浴阳等,海洋开发与管理,增刊,第78-82页,2013年01月", 《海洋开发与管理》 *
张诗泽等: "鹿回头多孔鹿角珊瑚与丛生盔形珊瑚性腺组织学研究", 《生态科学》 *
李春林等: "《国际环境法中的差别待遇研究》", 28 February 2013, 中国法制出版社 *
杨小东等: ""不同光照对稀杯盔形珊瑚生长及钙化的影响",杨小东等,海洋环境科学,第36卷第4期,第523-530页,2017年8月", 《海洋环境科学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110999824A (en) * 2019-11-19 2020-04-14 东南大学 Fixing device and fixing method for universal transplanted coral
CN115024260A (en) * 2022-07-06 2022-09-09 中国科学院南海海洋研究所 Separable coral transplantation reef body

Also Published As

Publication number Publication date
CN109169413B (en) 2020-11-27

Similar Documents

Publication Publication Date Title
CN101803581B (en) Method for cultivating large granular nuclear pearls
CN106342732A (en) Loach fry culture method
CN101120655B (en) Method for making potamogeton cripus artificial seed
CN102301878A (en) Method for evaluating drought resistance/drought tolerance of sugarcane seedlings at seedling stage
CN109169413A (en) A kind of separation method and transplantation method of the single anthocyathus of helmet shape coral
CN101422128B (en) Separation and regeneration method of gulfweed protoplast
CN104026082B (en) A kind of sexual artificial fecundation method of project cost consulting item
de Voogd The mariculture potential of the Indonesian reef-dwelling sponge Callyspongia (Euplacella) biru: growth, survival and bioactive compounds
CN105660357A (en) Artificial half-salt water ecological breeding method of enteromorpha
CN102823414A (en) Non-tube cutting seedling method of tissue subcultured buds of zenia
CN106116800B (en) A kind of bamboo willow culture solution and preparation method thereof and bamboo willow inoculation method
CN105028274B (en) A kind of pearl pearl culturing method
CN115250915B (en) Efficient propagation method for inducing protocorm and plant regeneration by stem segments of dendrobium nobile
CN2617130Y (en) Underwater vegetation planter in lake
CN106613351B (en) A kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant
CN106069922A (en) A kind of method improving Concha Tridacna squamosa larval metamorphosis rate
Bird et al. Salinity effects on Ruppia maritima L. cultured in vitro
Dunn et al. The history of Murdannia keisak (Commelinaceae) in the southeastern United States
Mickelson Defining culture requirements for reproduction and growth of Asparagopsis taxiformis, a Hawaiian native red alga
CN102487898B (en) Culture method and culture device used before attachment of coral oosperms
CN106069940A (en) A kind of cave coral indoor sexual reproduction method
CN101486991B (en) Method for propagating Tortula muralis protonema by cell engineering cultivation
Wagner Coral Reef Restoration Methods in the Caribbean and Florida Keys
KR20150116644A (en) Oyster larva's seed collecting device
CN103636547B (en) Method for acquiring mature eggs of aurelia

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant