CN109169413B - Separation method and transplantation method of single coral cup of helmet-shaped coral - Google Patents
Separation method and transplantation method of single coral cup of helmet-shaped coral Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a separation method and a transplanting method of a single coral cup of helmet-shaped coral, and belongs to the technical field of ecological artificial restoration of coral reefs. The separation method comprises the following steps: cutting the helmet-shaped coral individual to be separated into two halves from the mature part of the middle coral cup, and separating at the cut part: the head of any coral cup is sleeved with a tubular appliance, the coral cup and the common bones of the coral cups at the periphery are broken by pressing down, and then the coral cup sleeved with the tubular appliance is broken off from the helmet-shaped coral individual. The separation method is simple, and can reduce the demand of coral matrix, reduce the damage to field coral and provide more culture monomers. The transplanting method comprises the following steps: the coral cup obtained by the above separation is fixed in a transplanting hole of a transplanting device, and the transplanting device is inserted into a coral reef in a transplanting area. The transplanting method is simple, greatly reduces the requirement on the coral matrix, can resist medium-level wave interference, and avoids death caused by the fact that the device rolls and dumps to press the coral.
Description
Technical Field
The invention belongs to the technical field of coral reef ecological artificial restoration, and particularly relates to a separation method and a transplanting method of a single coral cup of helmet-shaped coral.
Background
Reef-building coral, which is an important structural organism of coral reefs, is currently listed in appendix two of International trade convention on endangered species of wild animals and plants, and is used as an important protected animal to be subject to trade control and protection. The number of reef-building corals on coral reefs is decreasing sharply due to threats from human activities, global climate changes, enemy organisms, marine pollution, etc. The current survey shows that over half of the coral reefs worldwide are severely degenerated and the degeneration process is accelerating, and scientists predict that the coral reef ecosystem may be extinct by the end of this century according to the current degeneration trend. Therefore, the protection of the hermatypic corals is more and more emphasized in various countries, the damage activities to the hermatypic corals and the coral trading behaviors are strictly controlled, and the research, development and popularization of the hermatypic coral cultivation technology are vigorously carried out.
The helmet-shaped coral is an important genus of hermatypic corals and is widely distributed in world coral reefs from the red sea, the southeast Asia sea and the great fort reef. In China, helmet-shaped corals grow from the coast of Fujian, Guangdong, Guangxi and Hainan to West sand islands and south sand islands. Because the helmet-shaped coral has strong tolerance to severe environment, the helmet-shaped coral is also one of the advantages of coral reefs especially in environments with more sediments and pollutants, such as the regions of the sky, the large-Asia bay, the three-gulf and the like. Due to the characteristic of strong tolerance, the coral culture medium becomes one of important coral species for researching the offshore polluted environment, and meanwhile, the protection and cultivation work for the helmet-shaped coral is gradually strengthened.
Because the protection for hermatypic corals is more and more strict, a coral experiment sample and a parent for culturing corals are required to be obtained from the field as little as possible, and the influence and damage to the field corals are reduced. Therefore, how to sample and cultivate the coral by adopting the minimum damage becomes the problem to be solved in the current coral experiment and coral cultivation.
Disclosure of Invention
The invention aims to provide a separation method of a helmet-shaped coral single coral cup, which is simple and can be used for reef-building coral experiments and artificial culture of helmet-shaped corals, so as to play roles in reducing coral parent demands, reducing damage to field corals and providing more culture monomers.
Another object of the present invention is to provide a method for transplanting a coral cup of a helmet-shaped coral, which is simple, can greatly reduce the demand for the coral mother, and can withstand moderate sea wave interference, and even if the device rolls or topples over, the coral will not be completely pressed by the device and will die.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
the embodiment of the invention provides a separation method of a single coral cup of helmet-shaped coral, which comprises the following steps:
cutting the helmet-shaped coral individual to be separated into two halves from the mature part of the middle coral cup, and separating at the cut part: the head of any coral cup is sleeved with a tubular appliance, the coral cup and the common bones of the coral cups at the periphery are broken by pressing down, and then the coral cup sleeved with the tubular appliance is broken off from the helmet-shaped coral individual.
In a preferred embodiment of the invention, the individual coral helmet to be isolated is obtained by the steps comprising: collecting wild healthy helmet-shaped coral individuals, pre-culturing in a culture environment, and taking the healthy helmet-shaped coral individuals obtained after the pre-culturing as helmet-shaped coral individuals to be separated.
Wherein, the farming environment includes: the difference between the temperature of the culture water body and the collecting temperature of the helmet-shaped coral individual is not more than 2 ℃, and/or the culture illumination intensity is 50-200 mu.mol.m-2·s-1And/or the culture salinity is 28-34.
In a preferred embodiment of the present invention, the living tissue of the wild healthy helmet-shaped coral individual collected is not less than 30cm in area2。
In a preferred embodiment of the invention, the inner diameter of the tubular device is 1-3mm larger than the diameter of the coral cup to be sheathed.
In a preferred embodiment of the invention, the wall thickness of the tubular device is not more than 0.5 mm.
The embodiment of the invention also provides a method for transplanting a single coral cup of helmet-shaped coral, which comprises the following steps: the coral cup separated by the above separation method is fixed in a transplanting hole of a transplanting device, and the transplanting device is inserted into a coral reef in a transplanting area in such a manner that one end to which the coral cup is fixed faces upward.
Transplanting the device and including connecting portion and 1 branch at least, the one end of every branch all is connected in connecting portion, and the tip of the one end of keeping away from connecting portion of 1 branch at least is seted up and is used for the transplantation hole of fixed coral cup.
In a preferred embodiment of the present invention, the transplanting device comprises a connecting part, a first branch, a second branch, a third branch and a fourth branch, wherein the second branch, the third branch and the fourth branch all extend from the connecting part to a direction away from the first branch and form a triangular frame shape to be fixed in the coral reef as a fixing foot, and one end of the first branch away from the connecting part is provided with a transplanting hole for fixing the coral cup.
In a preferred embodiment of the invention, each branch is 3-10cm in length.
In a preferred embodiment of the present invention, the diameter of the graft hole is 5 to 8mm and the depth is not less than 1 cm.
In a preferred embodiment of the present invention, when the coral cup is fixed in the graft hole in the air, the fixing operation is completed within 20min, and then the graft device with the coral cup fixed therein is put into the sea water.
The separation method and the transplanting method of the single coral cup of the helmet-shaped coral in the embodiment of the invention have the beneficial effects that:
the method for separating the helmet-shaped coral single coral cup provided by the embodiment of the invention is simple to operate, can be used for reef-building coral experiments and artificial culture of helmet-shaped corals, and can play a role in reducing coral parent demands, reducing damage to field corals and providing more culture monomers.
The transplanting method of the single coral cup of the helmet-shaped coral can greatly reduce the requirement on the coral parent body, can resist moderate sea wave interference, and even if the device rolls and topples over, the coral cannot be completely pressed by the device to cause death.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic structural diagram of a transplantation device having four branches according to an embodiment of the present disclosure.
Icon: 10-a grafting device; 11-a connecting portion; 15-branch; 151-first branch; 152-a second branch; 153-third branch; 154-fourth branch; 20-grafting holes.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships conventionally put in use of products of the present invention, and are only for convenience of description and simplification of description, but do not indicate or imply that the devices or elements referred to must have specific orientations, be constructed in specific orientations, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
Furthermore, the term "vertical" or the like does not require that the components be perfectly vertical, but rather may be slightly inclined. For example, "vertical" merely means that the direction is more vertical than "horizontal", and does not mean that the structure must be perfectly vertical, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," and "connected" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following examples are given to illustrate the present invention.
Examples
The separation method of the helmet-shaped coral single coral cup can comprise the following steps: cutting the helmet-shaped coral individual to be separated into two halves from the mature part of the middle coral cup, and separating at the cut part: the head of any coral cup is sleeved with a tubular appliance (such as a stainless steel pipe, hereinafter referred to as a steel pipe), the head is pressed downwards to break the common bone between the coral cup and the surrounding coral cups, and then the coral cup sleeved with the tubular appliance is pulled out from the helmet-shaped coral individual.
Wherein, the helmet-shaped coral individual to be separated can be obtained by the following steps: collecting wild healthy helmet-shaped coral individuals, pre-culturing in a culture environment, and taking the healthy helmet-shaped coral individuals obtained after the pre-culturing as helmet-shaped coral individuals to be separated.
In the present application, "healthy" may be, but not limited to, the judgment criterion of absence of symptoms of albinism, parasite or hydranth atrophy and partial tissue death in coral individuals, and may include other judgment criteria known in the art.
In the application, the living tissue area of the collected wild healthy helmet-shaped coral individual is not less than 30cm2So as to avoid that the separated coral cups are small and unfavorable for survival. Specifically, the above or tissue area may be 30cm2、32cm2、34cm2、35cm2、36cm2、38cm2Or 40cm2And the like.
Optionally, the temperature of the culture water body in the culture environment is different from the collecting temperature of the helmet-shaped coral individual by no more than 2 ℃, preferably no more than 1 ℃, so as to avoid the phenomenon that the temperature difference between the culture water body and the helmet-shaped coral individual is too large, so that the adaptability of the coral is reduced, stress is caused, and whitening, diseases, death and the like occur. The aquaculture water body can be long-flowing water or circulating water, and is preferably long-flowing water (the long-flowing water means that new water body continuously flows in and old water body continuously flows out in the aquaculture process).
Alternatively, the cultivation illumination intensity in the cultivation environment can be 50-200 mu. mol.m-2·s-1For example 50. mu. mol. m-2·s-1、60μ·mol·m-2·s-1、80μ·mol·m-2·s-1、100μ·mol·m-2·s-1、120μ·mol·m-2·s-1、140μ·mol·m-2·s-1、160μ·mol·m-2·s-1、180μ·mol·m-2·s-1Or 200. mu. mol. m-2·s-1And the like. The illumination intensity range is more suitable for survival of the helmet-shaped coral, and the coral is whitened easily due to overhigh illumination intensity; too low illumination intensity can reduce photosynthesis of symbiotic algae in corals, so that sufficient energy substances cannot be produced to supply the corals, and the corals grow slowly or the body is degenerated, atrophied and even die.
Alternatively, salinity in the aquaculture environment may be 28-34, such as 28, 28.5, 29, 29.5, 30, 30.5, 31, 31.5, 32, 32.5, 33, 33.5, or 34, and the like. The salinity refers to the ratio of the mass of dissolved substances to the mass of water. The salinity is controlled in the range, so that the cell wall osmotic pressure can be effectively prevented from being increased to crack and lose the original function.
The pre-culture time can be 7-15 days, such as 7 days, 8 days, 10 days, 12 days, 14 days or 15 days, etc., and the coral is suitable for new culture environment by pre-culture under the conditions, so that the death of separated coral cups caused by drastic environmental change is avoided.
And observing whether unhealthy appearances such as albinism, pathological changes or hydranth atrophy and the like of the coral individual appear after pre-culture, taking the healthy helmet-shaped coral individual obtained after the pre-culture as the helmet-shaped coral individual to be separated, wherein the healthy helmet-shaped coral individual shows the healthy states such as hydranth tentacles spread and normal coral color and luster and the like. It is worth saying that when the pre-cultured coral is unhealthy, the culture conditions can be adjusted in time until the coral is healthy.
The coral helmet individuals may be sterilized to remove germs, parasites, etc. that they contain or may potentially have before being chiseled in half.
During a particular operation, an individual of a helmet-shaped coral to be separated may be cut in half from the mature (preferably the most mature) position of the development of the median coral cup using, for example, a hammer and a flat chisel, and separated from the cut.
Preferably, in this embodiment, the tubular device is a hollow tubular device, and the material of the tubular device is not particularly limited. The inner diameter of the tubular appliance is 1-3mm larger than the diameter of the coral cup to be sleeved, so that on one hand, the head of the coral cup exposed outside can be conveniently and completely sleeved, and the head of the coral cup cannot be damaged or rubbed; on the other hand, the coral cup can be fixed to a certain extent so as to avoid falling off from the tubular appliance in the subsequent operation process.
In the case of a conventional coral cup, the inner diameter of the tubular device may be 6-10mm, such as 6mm, 6.5mm, 7mm, 7.5mm, 8mm, 8.5mm, 9mm, 9.5mm, or 10mm, respectively. Furthermore, the wall thickness of the tubular appliance is preferably not more than 0.5mm, such as 0.5mm, 0.45mm, 0.4mm, 0.35mm, or 0.3mm, etc.
After the tubular appliance is sleeved on the head of any coral cup at the fracture, the coral cup is pressed vertically and forcibly for 1-2cm to destroy the common bones between the coral cup and the coral cups at the periphery of the coral cup, and then the coral cup sleeved by the tubular appliance can be pulled out from the helmet-shaped coral individual by slightly pulling the coral cup outwards.
The separated coral cup can be extracted from the tubular tool (e.g., steel tube) by: pinching the coral skeleton exposed outside the tubular device, slightly extracting from the tubular device, immediately placing in the culture seawater, and vertically fixing in a prepared base to avoid horizontal placement or inverted placement. After temporary culture for 7 days, if no phenomena such as death, tissue atrophy, color change and mucus secretion and the like occur, the helmet-shaped coral cup obtained by separation can be used for coral experiments or coral culture.
It should be noted that the coral cup separation in this application can also be achieved by directly using a separation tool such as tweezers or nipper pliers to pry the individual coral cups from the parent coral, but preferably using a tubular instrument. When the coral cup is pried, the coral tissues on the coral cup are prevented from being injured as much as possible, and the cylindrical shape of the coral cup is kept. And (3) putting the separated individual coral cups in seawater in time to ensure the survival of the coral cups, and taking out the coral cups when the coral cups need to be used or transplanted.
Further, when a plurality of coral cups are required, the coral cups are repeatedly separated at the cut-out portion cut by the separation tool according to the above separation method.
In conclusion, hundreds of coral cups with the same genotype can be obtained from a single coral mother body by adopting the single-cup separation method, and can be used for comparison experiments of coral effects caused by different environmental influences in coral experiments due to the fact that the genotypes of the coral cups are the same. In addition, the method can also separate coral cups with different genotypes from different coral matrixes so as to be used for a contrast experiment on the influence of different corals under the same environment in a coral experiment. Meanwhile, the coral cups obtained by separation by the separation method have the capability of growing new coral individuals, so that the demand on field corals in coral culture can be effectively reduced, and the number of field corals is indirectly protected.
Further, the application also provides a method for transplanting the single coral cup of the helmet-shaped coral, which can comprise the following steps: the coral cup separated by the method for separating individual coral cups of the helmet-shaped coral described above is fixed in the transplanting hole 20 of the transplanting apparatus 10, and the transplanting apparatus 10 is inserted into the coral reef in the transplanting area so that one end to which the coral cup is fixed faces upward.
Specifically, the coral cup can be secured within the graft well 20 of the graft device 10 by an adhesive (e.g., a single coral cup can be inserted into the graft well 20 of the graft device 10 and then secured thereto by the adhesive). The adhesive is a water environment-applicable glue, namely a glue substance which can be used in water, and can comprise an underwater epoxy resin glue or an underwater jelly glue, for example.
The transplantation can be carried out in seawater or air, when the coral cups are fixed in the transplantation holes 20 and carried out in air, the fixing operation is preferably controlled to be completed within 20min, and then the transplantation device 10 fixed with the coral cups is put into seawater to reduce the exposure time of the transplantation device in the air, so that the coral cups are prevented from being easily stressed and dead after being transferred into the seawater after the air temperature is obviously higher than the seawater temperature.
In the present application, the transplanting device 10 includes a connecting portion 11 and at least 1 branch 15, one end of each branch 15 is connected to the connecting portion 11, and a transplanting hole 20 for fixing a coral cup is opened at one end of the at least 1 branch 15 far from the connecting portion 11.
Alternatively, the number of branches 15 may be 1, 2, 3, 4, etc. When the number of the branches 15 is only 1 or the number of the branches 15 is plural and the plural branches 15 extend from the connecting portion 11 to the upper side of the connecting portion 11, the connecting portion 11 can be fixed on the reef bottom by a cable tie or pressed by stone to play a role of fixing.
When the number of the branches 15 is plural, at least 1 branch 15 may be fixed in the coral reef as a fixing leg without the transplanting hole 20, and the remaining branches 15 may extend from the connecting portion 11 to the upper side of the connecting portion 11 as a transplanting leg and have the transplanting hole 20 at the end thereof, that is, the fixing leg and the transplanting leg extend toward the upper and lower sides of the connecting portion 11, respectively.
For reference, the transplanting device 10 includes a connecting portion 11, a first branch 151, a second branch 152, a third branch 153, and a fourth branch 154 (see fig. 1), wherein the second branch 152, the third branch 153, and the fourth branch 154 each extend from the connecting portion 11 in a direction away from the first branch 151 (i.e., below the connecting portion 11) and form a triangular frame shape to be fixed in the coral reef as a fixing leg, and the first branch 151 extends from the connecting portion 11 to above the connecting portion 11 and is provided at one end thereof away from the connecting portion 11 with a transplanting hole 20 for fixing a coral cup. It is noted that the placement of the transplantation device 10 is avoided as much as possible on the sand bottom and the silt, and the placement depth can be controlled to 2-15 m.
The length of each branch 15 of the above described graft device 10 may, by reference, be 3-10cm, such as 3cm, 4cm, 5cm, 6cm, 7cm, 8cm, 9cm or 10cm, or may be combined in the range of 3-10 cm.
The diameter of the implantation hole 20 may be 5 to 8mm, such as 5cm, 5.5cm, 6cm, 6.5cm, 7cm, 7.5cm or 8cm, and the depth of the opening is not less than 1 cm.
Bearing on, through being fixed in transplanting the hole 20 of transplanting the device 10 with the coral cup that the separation gained, place transplanting the device 10 wholly in the transplantation region again, can resist moderate sea wave and disturb, even the device produces and rolls and topples over, the coral can not pressed down by the device completely yet, causes the death.
Test examples
The coral transplantation is carried out on the red deer coral reef, the separation and transplantation method of the single helmet-shaped coral cup provided by the application is adopted to collect helmet-shaped coral individuals and decompose the individual helmet-shaped coral cups into the single coral cups to be fixed on the transplantation device, the survival rate of the single helmet-shaped coral cup obtained by decomposition for 15 days is 92%, the survival single coral cup is good, and the method can be used for experiment and coral cultivation. Transplanting the same number of coral individuals, wherein the number of coral parents is only 3% of that of conventional transplants. The coral grows for 3 months, the multiplication of a single coral cup is 12-34 coral cups, and the growth rate is more than 15 times of that of the traditional method. The survival rate of the transplanted tile is 95%, and is improved by about 12% compared with the survival rate of 83% of a single helmet-shaped coral cup transplanted on the surface of the tile.
In conclusion, the method for separating the helmet-shaped coral single coral cup provided by the embodiment of the invention is simple to operate, can be used for reef-building coral experiments and artificial culture of helmet-shaped corals, and can play roles in reducing coral parent demands, reducing damage to field corals and providing more culture monomers. The transplanting method of the single coral cup of the helmet-shaped coral can greatly reduce the requirement on the coral parent body, can resist moderate sea wave interference, and even if the device rolls and topples over, the coral cannot be completely pressed by the device to cause death.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A method for transplanting a single coral cup of helmet-shaped coral is characterized by comprising the following steps: fixing the separated coral cup in a transplanting hole of a transplanting device, and inserting the transplanting device into a coral reef in a transplanting area in a mode that one end fixed with the coral cup is upward;
the transplanting device comprises a connecting part and at least 1 branch, one end of each branch is connected with the connecting part, and the end part of one end of each 1 branch, which is far away from the connecting part, is provided with the transplanting hole for fixing the coral cup;
the separation method of the coral cup comprises the following steps:
cutting the helmet-shaped coral individual to be separated into two halves from the mature part of the middle coral cup, and separating at the cut part: sleeving the head of any coral cup by using a tubular appliance, pressing down to break the common bone between the coral cup and the coral cups at the periphery, and then breaking out the coral cup sleeved by the tubular appliance from the helmet-shaped coral individual;
keeping the cylindrical shape of the coral cup during separation;
the inner diameter of the tubular appliance is 1-3mm larger than the diameter of the coral cup to be sleeved.
2. The grafting method of claim 1, wherein the individual coralliform coral to be isolated is obtained by a process comprising: collecting wild healthy helmet-shaped coral individuals, pre-culturing in a culture environment, and taking the healthy helmet-shaped coral individuals obtained after the pre-culturing as the helmet-shaped coral individuals to be separated;
wherein, the farming environment includes: the difference between the temperature of the culture water body and the collection temperature of the helmet-shaped coral individual is not more than 2 ℃, and/or the culture illumination intensity is 50-200 mu.mol.m-2·s-1And/or the culture salinity is 28-34.
3. The transplantation method as set forth in claim 2, wherein the living tissue of the wild-type healthy helmet-shaped coral individual is collected over an area of not less than 30cm2。
4. The grafting method of claim 1, wherein the tubular appliance has a wall thickness of no greater than 0.5 mm.
5. The transplanting method according to claim 1, wherein the transplanting device comprises a connecting portion, a first branch, a second branch, a third branch and a fourth branch, wherein the second branch, the third branch and the fourth branch all extend towards a direction away from the first branch from the connecting portion and form a triangular frame shape to be fixed in a coral reef as a fixing foot, and one end of the first branch away from the connecting portion is provided with the transplanting hole and used for fixing the coral cup.
6. The method of claim 5, wherein each of said branches is 3-10cm in length.
7. The grafting method according to claim 1, wherein the grafting holes have a diameter of 5 to 8mm and a depth of not less than 1 cm.
8. The transplanting method according to claim 1, wherein when the coral cup is fixed in the transplanting hole in the air, the fixing operation is completed within 20min, and then the transplanting apparatus with the coral cup fixed thereto is put into seawater.
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| CN110999824A (en) * | 2019-11-19 | 2020-04-14 | 东南大学 | Fixing device and fixing method for universal transplanted coral |
| CN115024260B (en) * | 2022-07-06 | 2023-07-28 | 中国科学院南海海洋研究所 | Separable coral transplanting reef body |
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