CN109168685A - The method and long shoot mun orchid cutting propagation matrix of long shoot mun orchid cutting propagation - Google Patents
The method and long shoot mun orchid cutting propagation matrix of long shoot mun orchid cutting propagation Download PDFInfo
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- CN109168685A CN109168685A CN201810895790.2A CN201810895790A CN109168685A CN 109168685 A CN109168685 A CN 109168685A CN 201810895790 A CN201810895790 A CN 201810895790A CN 109168685 A CN109168685 A CN 109168685A
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- cuttings
- long shoot
- cuttage
- cutting
- mun orchid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method of long shoot mun orchid cutting propagation and long shoot mun orchid cutting propagation matrix, the method for the long shoot mun orchid cutting propagation comprising steps of interception long shoot mun orchid branch, remove the stipes for growing inflorescence in branch, be cut into cuttings；It will be in the cutting medium of cuttings cuttage to wetted treated long shoot mun orchid；Cuttage management processing is carried out to cuttings of the cuttage into cutting medium, including foliar fertilizer is sprayed to cuttings.The method of long shoot mun orchid cutting propagation of the present invention is reproductive tissue using the branch of long shoot mun orchid, is bred using cuttage mode, is effectively avoided existing using seed, division propagation low efficiency and the demanding deficiency of Reproduction Conditions.In addition, the cutting medium of long shoot mun orchid cuttings cuttage to the long shoot mun orchid of selection is bred, to effectively increase reproductive survival rate, and the stabilization of kind can guarantee.
Description
Technical field
The invention belongs to plant tissue reproduction technique fields, and in particular to a kind of method of long shoot mun orchid cutting propagation and
Long shoot mun orchid cutting propagation matrix.
Background technique
Long shoot mun orchid (Phaiuslongicruris Z.H.Tsi) is that pseudobulb is cylindrical, 20-40 centimetres long, slightly
1.3-2 centimetres, tool 3-8 section, 5-7 centimetres of internode length.Usual 6 pieces of leaf, mutually it is born in pseudobulb top, blade ellipse or ellipticity
Lanceolar, scape normal 2, from the section of base portion or nearly base portion on extract out, be up to 45 centimetres；Thick 5 millimeters of common peduncle, 3-5 pieces of sparsely grow
Sheath, it is hairless；The a small number of flowers of raceme tool spend medium big, opening, sepal and petal pistac；Petal is hairless, and ovate drapes over one's shoulders needle
Shape, the nearly ovate triangle of pollen load；The month at florescence 8-10.Long shoot mun orchid originates from Chinese yunnan south (Jinghong, Menla), is born in sea
It pulls out under 1000-1400 meters of cheuch thick forest, for national II grade of protection plant, Chinese unique wheat, this kind has cultivation, with higher
Horticultural importance.
For long shoot mun orchid as other orchids, seed development is incomplete, sprouts without promising seed and provides nutrition
Endosperm or other trophic structures, the time for needing extremely to grow by seminal propagation under field conditions (factors) could obtain the plant that blooms,
And germination percentage and survival rate are all extremely low.In addition, due to the destruction of excessive digging and habitat for a long time, long shoot mun orchid
Wild resource largely reduce, be in Critical Condition at present, be only unable to satisfy people to mun orchid by wild resource
Demand.In recent years, people attempt to use division propagation, but the breeding coefficient of division propagation is also very low, the speed of growth
It is extremely slow, it cannot achieve mass propagation in the short time, and this modes of reproduction makes the easy to carry virus of seedling, and kind is caused to be moved back
Change, seriously limits its commercialization.
Summary of the invention
It is an object of the invention to overcome the above-mentioned deficiency of the prior art, a kind of side of long shoot mun orchid cutting propagation is provided
Method, to solve, current long shoot mun orchid reproductive efficiency is low and the demanding technical problem of Reproduction Conditions.
In order to achieve the above-mentioned object of the invention, an aspect of of the present present invention provides a kind of side of long shoot mun orchid cutting propagation
The method of method, the long shoot mun orchid cutting propagation includes the following steps:
The branch for intercepting long shoot mun orchid, removes the stipes for growing inflorescence in the branch, removing was grown inflorescence
Stipes after the branch be cut into cuttings；
It will be in the cutting medium of the obtained cuttings cuttage of cutting to wetted treated long shoot mun orchid；
Cuttage management processing is carried out to the cuttings of the cuttage into the cutting medium, the cuttage management processing includes
Foliar fertilizer is sprayed to the cuttings.
Another aspect of the present invention provides a kind of long shoot mun orchid cutting propagation matrix, the long shoot mun orchid cuttage
Propagation substrate includes orchid stone, lime stone, porous-stone, pelelith, the mixture for planting one of metal and stone rubble or several rubbles.
Compared with prior art, the method for long shoot mun orchid cutting propagation of the present invention is numerous using the branch of long shoot mun orchid
Tissue is grown, is bred using cuttage mode, existing use seed, division propagation low efficiency and Reproduction Conditions is effectively avoided and wants
Seek high deficiency.In addition, by being bred in the cutting medium of the long shoot mun orchid cuttings cuttage of selection to long shoot mun orchid, from
And reproductive survival rate is effectively increased, and can guarantee the stabilization of kind.
Long shoot mun orchid cutting propagation matrix of the present invention uses specific rubble for raw material, so as to guarantee long shoot Heding
Blue cuttings implantation is taken root, and the survival rate of long shoot mun orchid cuttings is improved, and can guarantee the stabilization of kind.
Detailed description of the invention
Fig. 1 is the process flow of long shoot mun orchid cuttage breeding method provided in an embodiment of the present invention.
Specific embodiment
In order to which technical problems, technical solutions and advantageous effects to be solved by the present invention are more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain
The present invention is not intended to limit the present invention.
Unless otherwise defined, otherwise all technical terms and scientific terms used herein have and institute of the embodiment of the present invention
Belong to technical field those of ordinary skill and is generally understood identical meaning.If the definition stated in this part be incorporated by reference
The definition stated in the patent of this paper, patent application, the patent application of announcement and other publications is opposite or other aspects
Inconsistent, the definition listed in this part is prior to the definition in being totally incorporated herein by reference.
On the one hand, the embodiment of the present invention provides a kind of method of long shoot mun orchid cutting propagation, the long shoot mun orchid skewer
The process flow of propagation method is inserted as shown in Figure 1, it includes the following steps:
S01: the branch of interception long shoot mun orchid removes the stipes for growing inflorescence in branch, removing was grown inflorescence
Stipes after branch be cut into cuttings；
S02: will be in the cutting medium of the obtained cuttings cuttage of cutting to wetted treated long shoot mun orchid；
S03: cuttage management processing is carried out to cuttings of the cuttage into cutting medium, cuttage management processing includes to cuttings
Spray foliar fertilizer.
Wherein, in above-mentioned steps S01, in order to improve cuttings quality, it is preferable that in the long shoot mun orchid branch that will be intercepted
When being cut into cuttings, the raw healthy branch of long shoot mun orchid 1-2 is chosen, the stipes for growing inflorescence in healthy branch is removed, it will
It removes the branch after growing the stipes of inflorescence and is cut into cuttings according to every 1-3 stipes, thus by cuttings size and matter
The control and selection of amount guarantee the survival rate for improving the cutting propagation of cuttings from reproductive tissue source.
In above-mentioned steps S02, cutting medium is laid to seedbed in advance, forms the seedbed of cuttings cuttage.Implement one
In example, as follows by cuttings cuttage into cutting medium: after cuttings cuttage is into cutting medium, cuttings and cutting medium shape
At seedbed bed surface angle be 10-30 degree, cuttings expose cutting medium length be 3-5cm, to guarantee the saturating of cuttings base portion
Water gas permeability avoids cuttings base portion from rotting.
Preferably, in another embodiment, cutting medium includes orchid stone, lime stone, porous-stone, pelelith, plants metal and stone
One of rubble or several rubbles mixture.The matrix can be conducive to cuttings cuttage and survive, and improve survival rate, avoid inserting
Fringe cuttage occurs to rot.It is further preferred that the partial size of gravel particle is not more than 10mm, to be conducive to cuttings and cutting medium
Close contact, guarantee cuttings ambient humidity, light and temperature.
In addition, cutting medium is laid to seedbed, it is preferable that in one embodiment, cutting medium is laid in seedbed
With a thickness of 3-15cm, which can effectively improve the survival rate of cuttings.
In step S02, the wettability treatment carried out to cutting medium be can be at directly with water to cutting medium sprinkling
Reason can also carry out spraying processing, so that cutting medium is sufficiently humidified so as to the liquid fertilizer for being conducive to cuttings absorption.
In above-mentioned steps S03, by the obtained cuttings cuttage of cutting to the cuttage of wetted treated long shoot mun orchid
In matrix, cuttage management processing is carried out to cuttings of the cuttage into cutting medium, when carrying out cuttage management processing, cuttings is sprayed
Foliar fertilizer is applied, to guarantee the survival rate of cuttings, finally obtains cuttings seedling.Preferably, in one embodiment, cuttage management is handled
Including carrying out spraying foliar fertilizer every three days to cuttings, to supplement fertilizer to cuttings in time, effectively, surviving for cuttings is improved
Rate.Preferably, added with taking root class plant growth regulator in foliar fertilizer, to guarantee taking root for cuttings, improve cuttings at
Motility rate.It is further preferred that the class plant growth regulator of taking root added in foliar fertilizer is 0-2mg/L gibberellin, 0-50mg/
L methyl α-naphthyl acetate or 0-50mg/L indolebutyric acid, to guarantee to insert by the foliar fertilizer added with class plant growth regulator of taking root
Fringe is taken root, and the survival rate of cuttings is further increased, while improving the efficiency of breeding.
In another embodiment, it is preferable that when carrying out cuttage management processing to cuttings of the cuttage into cutting medium, also wrap
The control to cuttings cuttage environment is included, foliar fertilizer is sprayed with cooperation, the synergistic function of two conditions is realized, further increases
The survival rate and growth rate of cuttings.It, will be in the breeding environment of cuttings when further preferably, to the control of cuttings cuttage environment
Obscurity is controlled to 60-80%, and specific such as 60%, humid control to 80-90% is specific such as 85%.
It sprouts to the stem eye that cuttage cuttings carries out cuttings after cuttage management is handled 30-45 days and starts to grow.Further real
It applies in example, further includes that cuttings seedling, which is transplanted, that is to say to the cuttings survived after carrying out cuttage management processing to cuttage cuttings.
Particularly cuttage seeding is transplanted after cuttings grows to 3-5cm.Such as in a particular embodiment, by cuttings seedling from seedbed
It takes out, transplants the upper basin culture into bark, turfy soil and the cultivation matrix three-in-one with river sand.
Therefore, the method for above-mentioned long shoot mun orchid cutting propagation is reproductive tissue using the branch of long shoot mun orchid, is used
Cuttage mode is bred, and the deficiency of existing long shoot mun orchid breeding is effectively avoided.In addition, the long shoot mun orchid of selection is inserted
The cutting medium of fringe cuttage to long shoot mun orchid is bred, to effectively increase reproductive survival rate, and can guarantee kind
Stablize.
On the other hand, the embodiment of the invention also provides long shoot mun orchid cutting propagation matrix, the long shoot mun orchid skewers
Slotting propagation substrate includes orchid stone, lime stone, porous-stone, pelelith, the mixing for planting one of metal and stone rubble or several rubbles
Object.
When long shoot mun orchid cutting propagation matrix is the mixture of a variety of rubbles, the long shoot mun orchid cutting propagation base
Matter can be the mixture of following volume ratio:
In one embodiment, the long shoot mun orchid cutting propagation matrix is orchid stone.
In another embodiment, the long shoot mun orchid cutting propagation matrix includes that volume ratio is lime stone: pelelith 5:1's
Mixture.Wherein it is preferred to which the partial size of gravel particle is not more than 10mm.In this way, long shoot mun orchid cutting propagation matrix is using special
Fixed rubble is raw material, so as to guarantee that long shoot mun orchid cuttings implantation is taken root, improves the survival rate of long shoot mun orchid cuttings,
And it can guarantee the stabilization of kind.
Now by taking the method for specific long shoot mun orchid cutting propagation as an example, the present invention will be described in further detail.
Embodiment 1
A kind of method for present embodiments providing long shoot mun orchid cutting propagation, the method for the long shoot mun orchid cutting propagation
Include the following steps:
S11. the preparation of cuttage material:
Long shoot mun orchid 1 year raw healthy branch is chosen, the removal of selected branch was grown to the stipes of inflorescence, according to every 2
It is spare that stipes is cut into cuttings；
S12. the laying of cutting medium and seedbed:
The orchid rubble that partial size is not more than 10mm is chosen, the seedbed with a thickness of 10cm is laid to；
S13. cuttage and induction:
The seedbed that will be laid in step S12, after sufficiently wetting of spraying water, by the cuttings in S11, with cuttings and seedbed bed surface 20
It spends in angle tilt insertion matrix, every cuttings matrix exposes outside 4cm as degree.It sprays every three days once added with gibberellin (GA)
1mg/L, the foliar fertilizer of methyl α-naphthyl acetate (NAA) 30mg/L, 40 days stem eyes, which sprout, to be started to grow, and seedbed is laid on obscurity 60%,
In the constant environment of humidity 85%；Bud ratio is 87%, without rotten.
S14. it transplants:
The cuttage seeding formed in step S13 grows to height 3-5cm, takes out from seedbed, transplanting to bark, turfy soil
In the cultivation matrix three-in-one with river sand, upper basin culture.
Embodiment 2
A kind of method for present embodiments providing long shoot mun orchid cutting propagation, the method for the long shoot mun orchid cutting propagation
Include the following steps:
S21. the preparation of cuttage material:
Long shoot mun orchid 2 years raw healthy branches are chosen, the removal of selected branch were grown to the stipes of inflorescence, according to every 2-3
It is spare that a stipes is cut into cuttings；
S22. the laying of cutting medium and seedbed:
Choose the lime stone that partial size is not more than 10mm: pelelith is laid to thickness according to the mixing rubble that volume ratio is 5:1
For the seedbed of 5cm；
S23. cuttage and induction:
The seedbed that will be laid in step S22, after sufficiently wetting of spraying water, by the cuttings in S21, with cuttings and seedbed bed surface 30
It spends in angle tilt insertion matrix, every cuttings matrix exposes outside 4-5cm as degree.It sprays every three days once added with gibberellin (GA)
2mg/L, the foliar fertilizer of methyl α-naphthyl acetate (NAA) 10mg/L, 30 days stem eyes, which sprout, to be started to grow, and seedbed is laid on obscurity 70%,
In the constant environment of humidity 80%；Bud ratio 95%, without rotten.
S24. it transplants:
The cuttage seeding formed in step S23 grows to height 3-5cm, takes out from seedbed, transplanting to bark, turfy soil
In the cultivation matrix three-in-one with river sand, upper basin culture.
Embodiment 3
A kind of method for present embodiments providing long shoot mun orchid cutting propagation, the method for the long shoot mun orchid cutting propagation
Include the following steps:
S31. the preparation of cuttage material:
Long shoot mun orchid 2 years raw healthy branches are chosen, the removal of selected branch were grown to the stipes of inflorescence, according to every 1-3
It is spare that a stipes is cut into cuttings；
S32. the laying of cutting medium and seedbed:
Choose partial size be not more than 10mm plant metal and stone: lime stone according to volume ratio be 1:1 mixing be laid to a thickness of
The seedbed of 10cm；
S33. cuttage and induction:
The seedbed that will be laid in step S32, after sufficiently wetting of spraying water, by the cuttings in S21, with cuttings and seedbed bed surface 10
It spends in angle tilt insertion matrix, every cuttings matrix exposes outside 4-5cm as degree.It sprays every three days once added with gibberellin (GA)
1.5mg/L, the foliar fertilizer of methyl α-naphthyl acetate (NAA) 30mg/L, 30 days stem eyes, which sprout, to be started to grow, and seedbed is laid on obscurity
80%, in the constant environment of humidity 80%；Bud ratio 84%, without rotten.
S34. it transplants:
The cuttage seeding formed in step S33 grows to height 3-5cm, takes out from seedbed, transplanting to bark, turfy soil
In the cultivation matrix three-in-one with river sand, upper basin culture.
Comparative example 1
A kind of method for present embodiments providing long shoot mun orchid cutting propagation, the side of the long shoot mun orchid cutting propagation
Method includes the following steps:
S11. the preparation of cuttage material:
Long shoot mun orchid 2 years raw healthy branches are chosen, the removal of selected branch were grown to the stipes of inflorescence, according to every 1-3
It is spare that a stipes is cut into cuttings；
S12. the laying of cutting medium and seedbed:
Choose partial size be not more than 10mm lime stone: peat soil according to volume ratio be 1:1 mixing be laid to a thickness of
The seedbed of 10cm；
S13. cuttage and induction:
The seedbed that will be laid in step S22, after sufficiently wetting of spraying water, by the cuttings in S21, with cuttings and seedbed bed surface 30
It spends in angle tilt insertion matrix, every cuttings matrix exposes outside 4-5cm as degree.It sprays every three days once added with gibberellin (GA)
2mg/L, the foliar fertilizer of methyl α-naphthyl acetate (NAA) 50mg/L, 30 days stem eyes, which sprout, to be started to grow, and seedbed is laid on obscurity 60%,
In the constant environment of humidity 85%；Bud ratio 34%, rotten 52%.
S14. it transplants:
The cuttage seeding formed in step S23 grows to height 3-5cm, takes out from seedbed, transplanting to bark, turfy soil
In the cultivation matrix three-in-one with river sand, upper basin culture.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (9)
1. a kind of method of long shoot mun orchid cutting propagation, which is characterized in that described method includes following steps:
The branch for intercepting long shoot mun orchid, removes the stipes that inflorescence was grown in the branch, will remove the stem for growing inflorescence
The branch after section is cut into cuttings；
It will be in the cutting medium of the obtained cuttings cuttage of cutting to wetted treated long shoot mun orchid；
Cuttage management processing is carried out to the cuttings of the cuttage into the cutting medium, the cuttage management processing includes to institute
It states cuttings and sprays foliar fertilizer.
2. the method according to claim 1, wherein the branch of interception long shoot mun orchid, removes in the branch
It is the step of stipes for growing inflorescence, the branch after removing to be grown to the stipes of inflorescence is cut into cuttings, as follows:
The raw healthy branch of long shoot mun orchid 1-2 is chosen, the stipes for growing inflorescence in the healthy branch is removed, is given birth to removing
The branch after growing the stipes of inflorescence is cut into the cuttings according to every 1-3 stipes.
3. -2 described in any item methods according to claim 1, which is characterized in that the cutting medium includes orchid stone, lime
Stone, porous-stone, pelelith, the mixture for planting one of metal and stone rubble or several rubbles.
4. according to the method described in claim 3, it is characterized in that, the partial size of the gravel particle is not more than 10mm and/or institute
State cutting medium laying with a thickness of 3-15cm.
5. -2 described in any item methods according to claim 1, which is characterized in that the cuttings cuttage for obtaining cutting to quilt
Step in the cutting medium of long shoot mun orchid after wettability treatment, comprising:
By in the cutting medium of the obtained cuttings cuttage of cutting to wetted treated long shoot mun orchid, so that described insert
The seedbed bed surface angle that fringe and the cutting medium are formed is 10-30 degree.
6. -2 described in any item methods according to claim 1, which is characterized in that cuttage into the cutting medium described in
Cuttings carries out the step of cuttage management processing, comprising:
The cuttings is carried out every three days to spray foliar fertilizer.
7. method described in any one of -2 items according to claim 1, which is characterized in that the foliar fertilizer includes 0-2mg/L red
Mycin, 0-50mg/L methyl α-naphthyl acetate or indolebutyric acid.
8. the described in any item methods in -2,4 and 7 according to claim 1, which is characterized in that cuttage into the cutting medium
The cuttings carry out cuttage management processing the step of, further includes:
Obscurity in the breeding environment of the cuttings is controlled to 60-80%, humid control to 80-90%.
9. a kind of long shoot mun orchid cutting propagation matrix, which is characterized in that the long shoot mun orchid cutting propagation matrix includes orchid
Hua Shi, lime stone, porous-stone, pelelith, the mixture for planting one of metal and stone rubble or several rubbles.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810895790.2A CN109168685B (en) | 2018-08-08 | 2018-08-08 | Cutting propagation method of cymbidium longipedunculatum and cutting propagation substrate of cymbidium longipedunculatum |
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|---|---|---|---|
| CN201810895790.2A CN109168685B (en) | 2018-08-08 | 2018-08-08 | Cutting propagation method of cymbidium longipedunculatum and cutting propagation substrate of cymbidium longipedunculatum |
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| CN109168685A true CN109168685A (en) | 2019-01-11 |
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2018
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| CN1631104A (en) * | 2004-12-24 | 2005-06-29 | 中国科学院武汉植物园 | Rapid breeding method of pnaius |
| CN105766656A (en) * | 2016-04-28 | 2016-07-20 | 广西壮族自治区药用植物园 | One-step seedling method for rapid propagation of pseudobulbe of phaius tankervilleae |
| CN106278631A (en) * | 2016-08-18 | 2017-01-04 | 安庆市宝风农业发展有限公司 | A kind of Flos micheliae Albae growing nursery and culture substrate and preparation method thereof |
| CN106613960A (en) * | 2016-10-28 | 2017-05-10 | 中国科学院华南植物园 | Rapid propagation method of paphiopedilum helenae callus regeneration system |
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