CN110122085A - A kind of tilia amurensis shoot cutting fast breeding method - Google Patents
A kind of tilia amurensis shoot cutting fast breeding method Download PDFInfo
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- CN110122085A CN110122085A CN201910448393.5A CN201910448393A CN110122085A CN 110122085 A CN110122085 A CN 110122085A CN 201910448393 A CN201910448393 A CN 201910448393A CN 110122085 A CN110122085 A CN 110122085A
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- tilia amurensis
- cuttings
- cuttage
- breeding method
- shoot cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention provides a kind of tilia amurensis shoot cutting fast breeding method, it is related to lime tree vegetative propagation technique field, choosing the raw tilia amurensis seedling base portion current year raw sprout tillers branch of 2-3 is cuttage material, trim cuttings, after cuttings is using 200mg/L NAA processing 0.5h, it is inserted into sand: coco bran: perlite: in the matrix that vermiculite=20:16:3:1 is mixed, manage after inserting.The rooting rate of tilia amurensis shoot cutting can be improved to 90.33% in method provided by the invention, can also increase root quantity and maximum root long length.
Description
Technical field
The present invention relates to lime tree vegetative propagation technique field, specifically a kind of tilia amurensis shoot cutting fast breeding method.
Background technique
Tilia amurensis (Tilia amurensis) is Tiliaceae (Tiliaceae) Tilia (Tilia L.) deciduous tree, is me
The precious protection plant of II grade of state.Its tree performance is graceful, and material is light and soft, spends full of fragrance, is excellent Landscape Trees, with material tree
Kind and nectariferous plant, there are also very big medical values for flower, root etc.；Separately some researches show that tilia amurensis absorbs SO in urban habitats2And
It is relatively strong to reduce environmental pollution ability, therefore, can be promoted as urban forests tree species.For many years, due to the mistake of people
Degree is felled, and afforests and work is fostered not obtain enough attention, is caused the quantity of this rare tree of tilia amurensis to fall sharply, is arranged
Enter rare and endangered tree species.Therefore, carrying out the research of tilia amurensis efficient breeding method will be helpful to that its artificial forest is promoted to cultivate, and alleviate wild
The protection pressure of resource.
Currently, the breeding of tilia amurensis is still based on seed propagation, since Tilia amusensis seed has hibernation feature, it is necessary to by certain
Specially treated and combine the long period lamination process could breaking dormancy, process is cumbersome, and speed of germination is slow, and easily causes out
Seedling is uneven, limits the cultivation of tilia amurensis seedling to a certain extent.Therefore, scholars are unfolded in terms of the vegetative propagation of tilia amurensis
Extensive research, wherein cutting propagation due to easy to operate, reproduction speed it is fast and by favor.Currently, the research weight of people
Select influence of the type for being concentrated mainly on cutting position, cuttings age, growth regulator to tilia amurensis cuttage；IBA can be mentioned effectively
The rooting rate of high tilia amurensis shoot cutting, the effect of shoot cutting are better than hardwood cutting, but its highest rooting rate only up to 63.07%,
Effect is undesirable.In addition, other tree species such as tilia miqueliana (Tilia miqueliana of some scholars to Tilia
Maxim.), Mount Taishan linden (Tilia taishanensis S.B.Ling) etc. expands research, but its rooting rate is respectively
43.0%, 68.5%, also undesirable, correlative study technology can not be used for reference on its sibling species tilia amurensis.
In order to protect this rare tree of tilia amurensis, quickening tilia amurensis breeds process, what exploration can be promoted in production practice
Quick-breeding method, the application growth regulator type and its concentration, the processing time and in terms of expand exploration, with
Phase finds out the Technique on Cuttage Propagation system of a set of maturation, provides technical support for the large-scale development of tilia amurensis.
Summary of the invention
The present invention provides a kind of tilia amurensis shoot cutting fast breeding method, and it is numerous to solve tilia amurensis shoot cutting in the prior art
The low technical problem of the rooting rate educated.
The present invention is implemented as follows: including the following steps:
(1) cuttage material selects
Choosing the raw seedling base portion current year raw sprout tillers branch of 2-3 is cuttage material；
(2) prepared by matrix
Sand is mixed, coco bran, perlite, vermiculite fills hole tray after disinfection, sprinkle profoundly water as cutting medium, spare；
(3) cuttings trimming and processing
The cuttage material of pruning modes step (1), each cuttings retain 2 blades；By treated, cuttings is soaked in plant
In object growth regulator；
(4) cuttage and insert after manage
In the cuttings insertion plug media that step (3) is obtained, sprinkle profoundly water；
After cuttage in 20d, covering shading net, relative air humidity >=95%, 36-45 DEG C of temperature；After cuttage root-taking, Kei is removed
Shading net, full exposure hardening.
It is Routine Management after cuttage 20d.Conventional depth, such as 4 ± 1cm may be selected in cuttage depth.
As a preferred embodiment, sand in cutting medium in step (2): coco bran: perlite: vermiculite=20:
16:3:1。
As a preferred embodiment, the plant growth regulator that step (2) uses is NAA.
As a preferred embodiment, the NAA concentration is 200mg/L.
As a preferred embodiment, cuttings soaking time is 0.5h in step (2).
Soaking depth is 1-2cm.
As a preferred embodiment, spraying one time 1% potassium dihydrogen phosphate every 2d when managing after inserting.
As a preferred embodiment, cuttings is trimmed to 8-12cm, upper end notch straight snips, inferior end notch is tiltedly cut.
As a preferred embodiment, before cuttage for 24 hours, matrix repeats to sterilize.
Beneficial effects of the present invention: it is tender that tilia amurensis shoot cutting fast breeding method provided by the present application is remarkably improved tilia amurensis
The rooting rate of branch cuttage has reached the requirement of the factorial production, can promote and apply in production to 90.33%.
Detailed description of the invention
Fig. 1 is the range analysis figure of the adventitious root quantity of tilia amurensis shoot cutting seedling；
Fig. 2 is the range analysis figure of the indefinite root long of longest of tilia amurensis shoot cutting seedling；
Fig. 3 is influence range analysis figure of each factor to root range；
Fig. 4 is 5 transplant survival effect pictures of processing.
Specific embodiment
Technical solution of the present invention is clearly and completely retouched below in conjunction with specific embodiments of the present invention and attached drawing
It states, it is clear that described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts,
It shall fall within the protection scope of the present invention.
A kind of tilia amurensis shoot cutting fast breeding method, includes the following steps:
(1) cuttage material selects
Choosing the raw seedling base portion current year raw sprout tillers branch of 2-3 is cuttage material；
(2) prepared by matrix
Sand is mixed, coco bran, perlite, vermiculite fills hole tray after disinfection, sprinkle profoundly water as cutting medium, spare；
(3) cuttings trimming and processing
The cuttage material of pruning modes step (1), each cuttings retain 2 blades；By treated, cuttings is soaked in plant
In object growth regulator；
(4) cuttage and insert after manage
In the cuttings insertion plug media that step (3) is obtained, sprinkle profoundly water；After cuttage in 20d, shading net, air are covered
Relative humidity >=95%, 36-45 DEG C of temperature；After cuttage root-taking, Kei removes shading net, full exposure hardening.It is conventional tube after cuttage 20d
Reason.
Further, the cutting medium of step (2) is sand: coco bran: perlite: vermiculite=20:16:3:1.
Further, the plant growth regulator that step (2) uses is NAA.
Further, the NAA concentration is 200mg/L.
Further, cuttings soaking time is 0.5h in step (2).
Further, when managing after inserting, one time 1% potassium dihydrogen phosphate is sprayed every 2d.
Further, cuttings is trimmed to 8-12cm, upper end notch straight snips, and inferior end notch is tiltedly cut.
Further, before cuttage for 24 hours, matrix repeats to sterilize.
Test example
1. materials and methods
1.1 test periods, place
Test in June in 2018 9 life Shandong Province, the base Xia Zhang, Tai'an greenhouse carry out, greenhouse building: steel frame
Structure, plastic foil scaffolding, top are covered with shading net, and auto spraying, water system is good, drains unobstructed, relative air humidity control
95% or more, temperature is controlled at 36-45 DEG C system.
1.2 test material
1.2.1 cuttings selects
Using the raw tilia amurensis seedling base portion current year raw sprout tillers branch of 2-3 as cuttage material.
1.2.2 plant growth regulator
Using IBA, NAA, No. ABT1 3 kinds of root-inducing powders.
1.3 test method
1.3.1 experimental design
Using L9(34) Orthogonal Experiment and Design, it is related to growth regulator A, concentration B, cutting medium C and processing time D tetra-
Factor, each factor set 3 levels, totally 9 processing, 50 cuttings of each processing, every to handle in triplicate, as test group,
Experimental design is referring to table 1.
1 tilia amurensis shoot cutting Orthogonal Experiment and Design of table
1.3.2 cutting medium design and the method for preparation
The cutting medium of test group includes three kinds: matrix 1, matrix 2 and matrix 3.Matrix 1 (C1) is coco bran: perlite: leech
Stone=16:3:1, matrix 2 (C2) are perlite；Matrix 3 (C3) is sand: coco bran: perlite: vermiculite=20:16:3:1.Each skewer
It inserts matrix mixing and is all made of 1000 times of liquid disinfectants of carbendazim after the completion, the cutting medium disinfected is dispensed into the hole tray in 50 holes
In, cutting medium thickness is not less than 8cm, sprinkles profoundly water, spare；1000 times of liquid of carbendazim are reused before cuttage for 24 hours and repeat sterilizing one
Time.
1.3.3 prepared by cuttings
Cutting length is trimmed to 8-12cm, upper end notch straight snips, and inferior end notch is tiltedly cut, 2 blades of each cuttings band, every place
Reason 150 scion of acquisition；Cuttage is directly carried out after the cuttings trimmed is handled with various concentration growth regulator.
1.3.4 cuttage and insert after manage
Pretreated cuttings is inserted into spare matrix, cuttage depth is 4 ± 1cm, is then sprinkled profoundly water, and by cuttings
It is compacted with the matrix around jack；Using auto spraying method moisturizing, make relative air humidity control 95% or more, temperature controls
At 36-45 DEG C and continue 20d, the potassium dihydrogen phosphate for being 1.0% every 2d mass percent concentration of sprinkling during the 20d
Solution；Routine Management after cuttage 20d.After cuttage root-taking, Kei removes shading net, carries out full exposure hardening.
The investigation of 1.4 indexs and data analysis
When investigation, the cuttings that will first take root is extracted together with matrix, and then cuttings, which is put into clear water, will be adhered to root system
Matrix rinse well after, reference standard index is counted, calculate rooting percent, total radical, the indefinite root long of longest.It is indefinite
The sum of radical (the indefinite root long of longest, root range)=each duplicate indefinite radical (the indefinite root long of longest, root range)/each duplicate
Cuttings of taking root number.Variance analysis and Multiple range test are carried out using SPSS20.0 software, Excel 2010 charts.Root system development is referred to
It marks and carries out membership function value calculating: R (Xj)=(Xj-Xmin)/(Xmax-Xmin), in formula: Xj is a certain finger of a certain processing
Scale value, Xmin, Xmax are respectively minimum value and maximum value of all processing in the index.
2 results and analysis
Influence of 2.1 different disposals to tilia amurensis shoot cutting rooting rate
2.1.1 the variance analysis of lower rooting percent is combined in each processing
Each processing cuttage root-taking situation is shown in Table 2.From table 2 it can be seen that the cuttings in 9 processing has adventitious root generation,
It is No. 5 processing that wherein rooting rate is highest, up to 90.33%, secondly for No. 1 processing and No. 7 processing, (the two rooting rate is respectively
80.33% and 79.67%, no significant difference), it is 54.33% that rooting rate is minimum to be handled for No. 4.Rooting rate is poor between each processing
Different situation is shown in Table 2.
Respectively influence of the processing to tilia amurensis shoot cutting rooting rate of table 2
Note: Ti (i=1,2,3) indicates that the average value under each factor respective horizontal, R are maximum and minimum level spacing
The identical expression difference of same column lowercase is not significant, and lowercase difference indicates significant difference (p < 0.05)
In range analysis, the size of a certain factor influence can be seen that by very poor R value, R value is bigger, represents the factor
Influence to research index is bigger.As can be seen from Table 2, in 3 experimental factors, the R value of cutting medium is maximum.Thus illustrate
Influence of the cutting medium to cutting plantation is the most significant；Secondly it is the concentration (R=12) of plant growth regulator, makes a living again
Long regulator handles time (R=10), and influence of the type of growth regulator to tilia amurensis cutting plantation is minimum (R=8).
2.1.2 impact analysis of each experimental factor to cutting plantation
Although range analysis can cannot reflect its error size and precision with the reflection test result of simple, intuitive, because
This, also needs to carry out variance analysis.It the results are shown in Table 3, in 4 experimental factors, cutting medium has extremely significant shadow to cutting plantation
It rings (P < 0.01), and the type of growth regulator, concentration and processing time do not make significant difference to cutting plantation.
The variance analysis of 3 tilia amurensis shoot cutting rooting rate of table
Further to each horizontal progress Multiple range test of cutting medium, it the results are shown in Table 4, cutting medium C2 and C3 difference is aobvious
It writes, but C1 and C2 and C3 difference is not significant.The best cutting medium for promoting tilia amurensis shoot cutting rooting rate is C3 (matrix
3), average rooting rate is up to 81.33%.
Influence of 4 cutting medium of table to tilia amurensis shoot cutting rooting rate
No. 5 processing (growth regulator NAA, concentration 200mg/L, cutting medium are matrix 3, handle time 0.5h) are raw
Root rate is 90.33%, and high survival rate after transplanting has reached the requirement of the factorial production, can promote and apply in production.
Influence of 2.2 different disposals to tilia amurensis shoot cutting seedling root growth index
2.2.1 influence of each experimental factor to adventitious root quantity
Refering to attached drawing 1, range analysis is carried out to the adventitious root quantity of tilia amurensis shoot cutting seedling, the results showed that, tilia amurensis cuttage
The adventitious root quantity of seedling is maximum (9.67) with the R value of cutting medium, illustrates that cutting medium is the master for influencing root system adventitious root quantity
Want factor.Secondly it is the concentration of growth regulator, adventitious root quantity is influenced the smallest for the processing time.Further to each factor
Variance analysis and Multiple range test are carried out, the results are shown in Table 5 and table 6.From the dense of cutting medium known to table 5 and table 6 and growth regulator
It spends the influence to adventitious root quantity and has reached extremely significant horizontal (p < 0.01), and growth regulator type and processing time are to indefinite
The influence of radical amount has then reached the level of signifiance (P < 0.05).
2.2.2 influence of each experimental factor to the indefinite root long of longest
It is long to the longest adventitious root of tilia amurensis shoot cutting seedling to carry out range analysis refering to attached drawing 2, the results showed that, tilia amurensis skewer
The indefinite root long of the longest of seedlings plugging is maximum (4.95) with the R value of cutting medium, illustrates that cutting medium is to influence root system longest adventitious root
Long principal element.Secondly it is growth regulator type, the indefinite root long of longest is influenced the smallest for the processing time.It is further right
Each factor carries out variance analysis and Multiple range test, as a result referring to table 5 and table 6.From table 5 and table 6 as it can be seen that cutting medium to longest not
The influence for determining root long has reached extremely significant horizontal (P < 0.01), and the type of growth regulator, concentration and processing time are to longest
Indefinite root long does not make significant difference.
2.2.3 influence of each experimental factor to root range
Refering to attached drawing 3, range analysis is carried out to the root range of tilia amurensis shoot cutting seedling, the results showed that, the root of tilia amurensis cuttage seeding
Width is still the R value maximum (1.58) with cutting medium, illustrates that cutting medium is the principal element for influencing root range.It secondly is growth
Modifier concentration influences root range the smallest for the processing time.Variance analysis and Multiple range test, knot further are carried out to each factor
Fruit is referring to table 5 and table 6.Table 5 and table 6 the results show that influence of the cutting medium to root range reached it is extremely significant it is horizontal (P <
0.01), and influence of the growth regulator concentration to root range has reached the level of signifiance (P < 0.05), growth regulator type and processing
Influence of the time to root range be not significant.
Variance analysis of the 5 different tests factor of table to tilia amurensis shoot cutting root system development Index Influence
6 different tests factor of table and the horizontal Multiple range test to tilia amurensis shoot cutting root system development Index Influence
By above-mentioned test result it is found that influencing maximum experimental factor to the root index of tilia amurensis shoot cutting seedling is cuttage
Matrix, its influence to each root index have reached the extremely significant or level of signifiance, and the concentration of growth regulator is to indefinite radical
Amount and root range influence significantly, and the type of plant growth regulator and processing time only have a significant impact adventitious root quantity, right
The indefinite root long of longest and root range influence little.
The overall merit of 2.3 each processing tilia amurensis shoot cutting seedling quality
In production practice, judge that a certain processing is optimal combination, not only to see rooting rate, also to see cuttage seeding
Root system quality, therefore, on the basis of the studies above result, further using Subordinate Function to the rooting rate of each processing,
Adventitious root quantity, longest adventitious root length and root range have carried out overall merit, the results are shown in Table 7.Take 4 index membership function values
For average value as reference standard, membership function value is bigger, illustrates that the effect of the processing is better.As can be seen from Table 7, No. 5 processing
Membership function value highest, illustrate No. 5 processing for tilia amurensis shoot cutting effects it is best, the shoot cutting suitable for tilia amurensis.
The membership function value and overall merit of each index of 7 different disposal of table
2.4 tilia amurensis shoot cutting rooting process
Based on tilia amurensis shoot cutting rooting types are taken root with callus, rarely seen a small amount of skin zone takes root.Cuttage 7d or so, under cuttings
Notch can form a large amount of white callus, and a small number of cuttings 10d or so after cuttage already bears adventitious root, and when 20d is existing
Part cuttings forms tufted young root, and the adventitious root quantity of 30d, cuttings continue to increase, and has a large amount of fibrous roots and generate.Refering to attached
Fig. 4, high survival rate after the cuttings transplanting of No. 5 processing, growing way are good.
Beneficial effects of the present invention: it is tender that tilia amurensis shoot cutting fast breeding method provided by the present application is remarkably improved tilia amurensis
The rooting rate of branch cuttage has reached the requirement of the factorial production, can promote and apply in production to 90.33%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of tilia amurensis shoot cutting fast breeding method, which comprises the steps of:
(1) cuttage material selects
Choosing the raw seedling base portion current year raw sprout tillers branch of 2-3 is cuttage material；
(2) prepared by matrix
Sand is mixed, coco bran, perlite, vermiculite fills hole tray after disinfection, sprinkle profoundly water as cutting medium, spare；
(3) cuttings trimming and processing
The cuttage material of pruning modes step (1), each cuttings retain 2 blades；By treated, cuttings is soaked in plant life
In long regulator；
(4) cuttage and insert after manage
In the cuttings insertion plug media that step (3) is obtained, sprinkle profoundly water；
After cuttage in 20d, covering shading net, relative air humidity >=95%, 36-45 DEG C of temperature；After cuttage root-taking, Kei is except shading
Net, full exposure hardening.
2. a kind of tilia amurensis shoot cutting fast breeding method according to claim 1, which is characterized in that the skewer of step (2)
Insert sand in matrix: coco bran: perlite: vermiculite=20:16:3:1.
3. a kind of tilia amurensis shoot cutting fast breeding method according to claim 1, which is characterized in that step (2) uses
Plant growth regulator be NAA.
4. a kind of tilia amurensis shoot cutting fast breeding method according to claim 3, which is characterized in that the NAA concentration
For 200mg/L.
5. a kind of tilia amurensis shoot cutting fast breeding method according to claim 2 or 3, which is characterized in that in step (2)
Cuttings soaking time is 0.5h.
6. a kind of tilia amurensis shoot cutting fast breeding method according to claim 5, which is characterized in that when being managed after inserting,
One time 1% potassium dihydrogen phosphate is sprayed every 2d.
7. a kind of tilia amurensis shoot cutting fast breeding method according to claim 6, which is characterized in that cuttings is trimmed to 8-
12cm, upper end notch straight snips, inferior end notch are tiltedly cut.
8. a kind of tilia amurensis shoot cutting fast breeding method according to claim 7, which is characterized in that before cuttage for 24 hours, base
Matter repeats to sterilize.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110547106A (en) * | 2019-09-23 | 2019-12-10 | 苏州大学 | Simple in-situ green branch cuttage method for Tilia miqueliana Maxim |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
| CN112273071A (en) * | 2020-10-29 | 2021-01-29 | 北华大学 | Method for promoting tilia amurensis twig cutting rooting |
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| CN103597976A (en) * | 2013-10-18 | 2014-02-26 | 赵刚 | Seedling cultivation and propagation method for tilia amurensis |
| CN107853046A (en) * | 2017-10-26 | 2018-03-30 | 吉安市林业科学研究所 | A kind of wet-land pine tree industrial cuttage method for culturing seedlings |
| CN108293454A (en) * | 2018-01-23 | 2018-07-20 | 广西正匠农业科技有限公司 | A kind of honeysuckle cuttage and seedling culture method |
| CN109566245A (en) * | 2019-01-14 | 2019-04-05 | 湖南正清制药集团股份有限公司 | A kind of method of quick breeding caulis sinomenii |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103597976A (en) * | 2013-10-18 | 2014-02-26 | 赵刚 | Seedling cultivation and propagation method for tilia amurensis |
| CN107853046A (en) * | 2017-10-26 | 2018-03-30 | 吉安市林业科学研究所 | A kind of wet-land pine tree industrial cuttage method for culturing seedlings |
| CN108293454A (en) * | 2018-01-23 | 2018-07-20 | 广西正匠农业科技有限公司 | A kind of honeysuckle cuttage and seedling culture method |
| CN109566245A (en) * | 2019-01-14 | 2019-04-05 | 湖南正清制药集团股份有限公司 | A kind of method of quick breeding caulis sinomenii |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110547106A (en) * | 2019-09-23 | 2019-12-10 | 苏州大学 | Simple in-situ green branch cuttage method for Tilia miqueliana Maxim |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
| CN112273071A (en) * | 2020-10-29 | 2021-01-29 | 北华大学 | Method for promoting tilia amurensis twig cutting rooting |
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