CN109937722A - It is a kind of to utilize saururus chinensis aerial stem of plant rapid propagation method - Google Patents

It is a kind of to utilize saururus chinensis aerial stem of plant rapid propagation method Download PDF

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CN109937722A
CN109937722A CN201910185736.3A CN201910185736A CN109937722A CN 109937722 A CN109937722 A CN 109937722A CN 201910185736 A CN201910185736 A CN 201910185736A CN 109937722 A CN109937722 A CN 109937722A
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stem
saururus chinensis
water
plant
utilize
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CN109937722B (en
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徐蕾
王发国
陈思宇
谢东升
刘东明
邢福武
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South China Botanical Garden of CAS
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Abstract

Saururus chinensis aerial stem of plant rapid propagation method is utilized the invention discloses a kind of, method includes the following steps: 1) obtain the saururus chinensis aerial stem of plant, retain upper soll layer and the most intensive stem section of air joining place section, stem is cut from nearly 25~30cm of base portion, remove most or all of leaf, obtains cutting;2) cutting level is disposed across in fertile water body or Huo Gelan nutrient solution, carries out suspension water planting 2~3 weeks, obtain shoot;3) by the shoot of acquisition by it is horizontal it is horizontal bury in a manner of be transplanted into the Nutrition Soil disinfected in advance and cultivate, obtain seedling;4) seedling of acquisition is subjected to division propagation, transplanting is cultivated in crop field or planting bag, is colonized.Saururus chinensis aerial stem of plant rapid propagation method of the invention improves at least 3 times than conventional vertical cuttage efficiency, is conducive to improve saururus chinensis reproductive efficiency, reduces Plant technique cost.

Description

It is a kind of to utilize saururus chinensis aerial stem of plant rapid propagation method
Technical field
The invention belongs to technical field of plant propagation, and in particular to a kind of side quickly bred using the saururus chinensis aerial stem of plant Method.
Background technique
Saururus chinensis (Saururus chinensis (Lour.) Baill.) is the perennial humidogene grass of Saururaceae Saururus This also known as side of a pond lotus root, water caulis akebiae, plain boiled water chicken etc..It is more than about 1 meter high;Stem thickness is strong, has the thick rib of lengthwise and groove, lower part to throw oneself on the ground, normal band White, top is upright, green.Leaf papery, it is wealthy oval to ovum shape lanceolar, it is 10-20 centimetres long, it is 5-10 centimetres wide, stem top 2-3 piece is often white in the florescence, is in petal-shaped, very beautiful.The month at florescence 4-6, inflorescence white are 12-20 centimetres long.Fruiting period 7-8 Month, fruit is subsphaeroidal, and about 3 millimeters of diameter, the more excipuliform protrusions in surface.Originate in Hebei, Shandong, Henan and the Yangtze river basin and its on the south it is each It saves, type specimen picks up near Guangzhou.Hygrophilous shade tolerant, impoverishment tolerant requires not tight, the shallow waters such as all sides of a pond, limes marginis, small stream side to soil Place or low-lying land can be grown.
Saururus chinensis herb is medicinal, containing multiple beneficials ingredients such as volatile oil, flavones, lignanoid, alkaloid, tannins, has anti- Inflammation, detoxification, hypoglycemic, liver protection, diuresis isoreactivity have significant effect to diabetes, urinary tract infections, lithangiuria etc. is treated and prevented Fruit.Saururus chinensis has antitumor action, and toxicity is smaller, also has potentiality in the prevention of cancer and early treatment.It can also make For the wild vegetable containing unique nutritional ingredient and ornamental flower, thus it is medical, edible, in terms of there is good open Hair prospect.
Currently, in terms of being concentrated mainly on chemical component and pharmacological action for the research of saururus chinensis both at home and abroad, it is white about three Research in terms of careless Lycopersicum esculentum almost blank, only publishing an article in 2000 Nian Jiangnian fine jade teachers, " saururus chinensis being planted experimentally and developing Using " brief introduction has been done to its rhizomatous division propagation method, Yu Chuntian takes band bud subterranean stem, is cut into 13-16cm long Root segment, planted by 30 × 20cm of distance between rows and hills, when plantation must make bud upward, and the method requires subterranean stem to have bud, and when cultivating Between be confined to spring;Publish an article within 2001 " tissue cultures of saururus chinensis " illustrate be with saururus chinensis rhizome, stem section and blade Explant, it is culture medium, the experimental method of evoked callus and the culture that suspends that MS, which adds different hormone combinations, the results showed that, The inducing effect of stem section is better than rhizome and blade, and the inductivity of callus is up to 81%.There is not specialised literature also at present The seminal propagation of saururus chinensis is studied, routinely seed propagation efficiency is lower.Three of the above method has certain limitation, to material Selection, planting time and environmental condition it is more demanding, be unfavorable for the quick breeding of saururus chinensis.The source master of saururus chinensis in the market If wild resource, artificial commerial growing is very few, is difficult largely to buy nursery stock.Mankind's exploitation and habitat destruction, lead to three White grass wild resource large area is reduced.Traditional modes of reproduction be difficult to meet in the market Various Seasonal to the need of saururus chinensis nursery stock It asks, limits application of the saururus chinensis on the landscape engineerings such as city trees and shrubs to a certain extent.
The study find that saururus chinensis is in spending a rear 1-2 month aerial parts to start to wither under natural conditions, blade is withered, ground Gradually hollow blackening is finally withered rotten for upper stem, waits spring of waiting for the coming year to continue to sprout, this characteristic yet there are no special literature record.
Saururus chinensis eats source forever as a kind of medicine, has both the indigenous plant of landscape function and economic value, studies from many aspects Its growth characteristics and Lycopersicum esculentum mode are conducive to the marketing and wild resource protection of saururus chinensis.
Summary of the invention
The purpose of the present invention is very few for the cultivation technique research of saururus chinensis correlation, wild resource is destroyed seriously, autumn Top is withered, and supply falls short of demand for medicinal material market, the status that garden seedling tree market is not yet opened, and provides a kind of simple and easy utilization three The method of stem breeding nursery stock on white meadow.
The present invention is realized by the following technical solution above-mentioned purpose:
It is a kind of to utilize saururus chinensis aerial stem of plant rapid propagation method, comprising the following steps:
(1) the saururus chinensis aerial stem of plant is obtained, upper soll layer and the most intensive stem section of air joining place section are retained, intercepts the aerial stem of plant The stem section of more than base portion to base portion 25~30cm removes most or all of leaf, obtains cutting;
(2) cutting level is disposed across in fertile water body or Huo Gelan nutrient solution, carries out suspension water planting 2~3 weeks, obtained Shoot;
(3) by the direct cultivating and growing of the shoot of acquisition or by it is horizontal it is horizontal bury in a manner of be transplanted into the nutrition disinfected in advance It is cultivated in soil, obtains seedling;
(4) seedling of acquisition is subjected to field planting cultivation.
The fertile water body of the step (2) can for fishpond water, pig farm breeding wastewater or water body containing Nutrition Soil etc., Water body mainly containing saururus chinensis nutrient elements required for the growth.
The Nutrition Soil of the step (3) is peat soil: pond sludge: husky by weight the mixture for being 5:2:1.
The step (3) is to Nutritive cube sterilization processing specifically: with every 25% metalaxyl wettable powder 5g, it is watered 5kg, Nutrition Soil 100kg is sprayed after mixing, mix is uniform in spray, accumulates 2h.
The horizontal horizontal thickness of earth-fill cover buried of the step (3) is 2cm, and cultivation spacing is 10cm.
It pours a water in step (3) cultivation at dusk daily, guarantees that moisture is sufficient, not have ponding, with defense sector Upper stem rots in soil.
The step (4) is to the high 25cm of seedling, according to shoot position by the seedling of acquisition is carried out field planting cultivation By stem section sub-cut at 2~3 sections, every 7~10cm of segment length handles notch with the diluted carbendazim of volume ratio 1:1000, then transplants It cultivated, be colonized in crop field or planting bag.
The transplanting is cultivated in big Tanaka, is colonized, and need to select to have abundant water resources, and the convenient field of irrigation and drainage is as crop field;? Big Tagayasu Den's whole the last week sprays a wide spectrum and goes out the herbicide of natural disposition;Crop field compartment face is flattened and is ditched when Tagayasu Den is whole greatly and sets compartment, Compartment face width is 1.5~2m, and the furrow width between compartment and compartment is 30~40cm, depth is 15~30cm;Cultivation spacing is 20~30cm, Thickness of earth-fill cover is 8~10cm;Just transplanting the last week respectively pours a water sooner or later toward compartment face, and injects water body into ditch, guarantees the depth of water It is the 1/2 of ditch depth;Transplanting fills the water in field after a week, so that water-holding capacity and compartment face in ditch is maintained an equal level, makes crop field moisture and soil moisture It is suitable with rice cultivation.
The transplanting of the step (4) is cultivated in planting bag, is colonized, and planting bag surrounding can be pricked hole, is placed in pond, bag The interior high 25cm of cultivation matrix, nursery stock thickness of earth-fill cover are 8~10cm, and the pond depth of water is 10cm;Transplanting first 3 days daily in planting bag A water is poured, a water supplement evaporation capacity is poured within every 2~3 days after transplanting, keeps water depth in the half of soil height.
The present invention selects the aerial stem of plant of 4~September acquisition saururus chinensis, wherein April is saururus chinensis flowering stage, and 5~June is to contain At the florescence, 7~September is the saururus chinensis also not serious withered stage after blossom fall, best with the branch effect of stalwartness in 5~July, therefore, excellent Selecting the aerial stem of plant for obtaining saururus chinensis 5~July is propagation material.
Compared with prior art, the invention has the following advantages:
(1) present invention is in 4~September, shallow cut or pulls up the saururus chinensis aerial stem of plant, retains upper soll layer and air joining place section most Intensive stem section is cut from nearly 25~30cm of base portion, advantageous using the aerial stem of plant at the basal part of stem to cutting of ground as cutting It sprouts new buds again at lateral bud and basal part of stem section, and the closely more intensive local rudiment power of base portion section is stronger;It is cut in the florescence Branch can be used as medicinal materials and fresh-cut floral material, and branch is sheared after blossom fall and is conducive to extend the picking time of saururus chinensis and watches Phase;The acquired saururus chinensis aerial stem of plant has very strong sprouting power of regeneration in water planting environment, and bud ratio is up to 100%, shoot The speed of growth is fast.
(2) present invention by the horizontal shallow embedding of the branch of germination in Nutrition Soil, has after the saururus chinensis aerial stem of plant water planting 2-3 weeks It is further sprouted conducive to sprouting, the transplanting of shoot base portion photosynthesis and nursery stock later period.
(3) present invention cultivates the saururus chinensis aerial stem of plant using horizontal cuttage mode, in germination percentage, germinative number and shoot Significantly it is better than vertical cuttage in terms of the speed of growth, the yield of bud is higher on every test branch, and growing way is more preferable, is conducive to three White grass growth and later period division propagation breed nursery stock with the method and improve at least 3 times than conventional vertical cuttage efficiency, be conducive to mention High seedling propagation efficiency reduces Plant technique cost.
Detailed description of the invention
Fig. 1 is saururus chinensis in May full-bloom stage landscape;
Fig. 2 is the healthy and strong saururus chinensis aerial stem of plant (right side) and withers the hollow aerial stem of plant (left side);
Fig. 3 is the initial growth situation of saururus chinensis branch (cutting) in embodiment 1;
Fig. 4 is the 5th week whole upgrowth situation of saururus chinensis branch in embodiment 1;
Fig. 5 is the 5th week local growth situation of saururus chinensis branch in embodiment 1;
Fig. 6 is the situation that saururus chinensis Miao Gang is transplanted into crop field in embodiment 2;
Fig. 7 is that saururus chinensis Miao Gang is transplanted into upgrowth situation of the crop field after 45 days in embodiment 2;
Fig. 8 is that water planting is moved back into Nutrition Soil (right height) and vertical cuttage (left short) comparison diagram in embodiment 4.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.In the following example not It indicates specific experiment condition and method, used technological means is usually conventional means well-known to those skilled in the art.
Embodiment 1
Saururus chinensis is acquired in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank on May 1st, 2018.The florescence is now in, Grow vigorous, the more consistent saururus chinensis nursery stock of growing way of choosing, stem rugosity about 1.5cm takes 25~30cm of its base portion stem section (i.e. From the stem section on the basal part of stem to base portion of ground between 25~30cm), remove all blades, retains bottom and save intensive section, with this Water culture experiment is carried out as cutting.Test water is pig farm breeding wastewater, is contained in blue water tank, every case is white with water 60L, three Careless cutting 15, traverse make its free buoyant growth in the water surface.Test period be 2 days-June 5 May in 2018, totally 5 weeks, often The growing state of all routine observation saururus chinensis, including germination percentage (germination branch number/total branch number) and height of seedling.Place is Sihui City The illumination of the village Nan Tang, draughty simple greenhouse's greenhouse, 25 DEG C of night temperature on average in canopy, daily mean temperature is 33 DEG C, average day It is 13h according to the time.
Water planting first week, for the germination percentage of the saururus chinensis aerial stem of plant up to 70%, the section budding of the nearly base portion of cutting was very fast;Second week, Germination percentage is up to 100%, and all sections all germinate in cutting, and blade is gradually spread out, and average height of seedling reaches 4cm;Third week, blade are further It is unfolded and grows up, average height of seedling 6.5cm;4th week, average height of seedling 11cm;5th week, New shoot growth was very vigorous, average Height of seedling reaches 17cm, test branch grows a large amount of new roots and bottom save intensive place sprout out it is new it is horizontal walk stem, biomass increases very Fastly.
The initial growth situation of above-mentioned saururus chinensis branch is shown in that Fig. 3, the whole upgrowth situation of the 5th week saururus chinensis branch are shown in Fig. 4, The local growth situation of 5th week saururus chinensis branch is shown in Fig. 5.
Embodiment 2
On August 1st, 2018 acquires saururus chinensis in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank.At this point, saururus chinensis It thanks about 1 month, slight withered and yellow phenomenon occurs in top blade, the more consistent saururus chinensis of growing way of choosing, and stem slightly about 2cm takes Its nearly base portion 30cm or so stem section (i.e. from the stem section on the basal part of stem to base portion of ground between 30cm or so), removes all blades, Retain bottom and save intensive section, (level is disposed across the water surface in paddy field in being transplanted into experimental plot at dusk in this, as cutting On).Test site is the village Sihui City Nan Tang, and test crop field is paddy field, and compartment face width is 1.5m, and the furrow width between compartment and compartment is 30cm, depth 20cm are planted, every row 4, every 25 row of compartment, 2 compartment totally by distance between rows and hills 40cm × 30cm, and thickness of earth-fill cover is 8~10cm. The before noon water filling in experimental plot in advance is transplanted, makes ground moistening convenient for branch insertion.It is filled the water in field after the completion of transplanting, makes ditch Interior water-holding capacity and compartment face maintain an equal level, and keep crop field moisture, soil moisture and rice cultivation suitable.Test period is August in 2018 1- October 15, the growing state of the saururus chinensis of observation in every 15 days, including (base portion budding branch number/test is total for base portion germination rate Branch number), lateral bud branch out rate (lateral bud sprout the total branch number of branch number/test), average longest shoot length (test branch Longest shoot length and the/total branch number of test) and it is average maximum leaf area (the total branch number of maximum leaf area/test of branch).8 1 day-September 30th moon, 26 DEG C of experimental plot night temperature on average, daily mean temperature are 34 DEG C;October 29 DEG C of daily mean temperature, night is average Temperature is 19 DEG C.August part be averaged sunshine when a length of 13h, September part be averaged sunshine when a length of 12.5h, October average sunshine time For 11.5h.
August 16 days, sprouting out was sprouted in saururus chinensis cutting in base portion soil, and base portion germination rate up to 50%, branch out rate by lateral bud It is 70%, most nearby saves from notch and send forth branches at first, average longest shoot length is 5cm, and average maximum leaf area is 9cm2; August heavy rain on the 30th is observed after becoming a fine day in September 1st, and the cuttage survival rate of aerial stem of plant branch is 100%, and base portion germination rate is 100%, and grow rapidly, lateral bud rate of branching out is 100%, and average longest shoot length is 9cm, and average maximum leaf area is 15cm2;September typhoon on the 16th brings heavy rain, observes after becoming a fine day in September 18th, and average longest shoot length is 15cm, average maximum Leaf area is 25cm2;October 3, average longest shoot length are 22cm, and average maximum leaf area is 30cm2.It put down October 15 Equal longest shoot length is 30cm, and the longest shoot of 90% branch is the branch that base portion is sprouted out, average maximum leaf area For 42cm2
The situation that above-mentioned saururus chinensis Miao Gang is transplanted into crop field is shown in Fig. 6, and saururus chinensis transplantation of seedlings enters growth shape of the crop field after 45 days Condition is shown in Fig. 7.
Embodiment 3
On August 15th, 2018 acquires the saururus chinensis aerial stem of plant in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank.At this point, Or so 1 half a month of saururus chinensis blossom fall, stem top blade is slightly withered and yellow, and the aerial stem of plant for selecting rugosity about 1cm more delicate intercepts it Base portion 25cm or so length stem section (i.e. from the stem section on the basal part of stem to base portion of ground between 25cm or so), removes all blades, Retain base portion section comparatively dense stem section, in this, as cutting in carrying out cultivation examination in South China Botanical Garden Chinese Academy of Sciences greenhouse It tests.Test period is August in 2018 15 days-September 12 days, totally 4 weeks, the growing state of routine observation saururus chinensis weekly, including send out Bud rate (germination branch number/total branch number), mean germination number (bud quantity summation/total branch number), (number of sheets amount is total for average leaf number With/total branch number) and average longest shoot length (the longest shoot length summation of observation branch/always branch number).August is per day Temperature is 33 DEG C, and night temperature on average is 26 DEG C, when average sunshine a length of 13h.
By peat soil: pond sludge: the husky ratio according to weight ratio 5:2:1 mixes, and being placed in diameter is 40cm, the plantation of high 30cm In bag, the deep about 25cm of cultivation matrix.Cutting level used will be tested to be disposed across on cultivation matrix, 3, every basin, totally 10 basin;It will kind It plants bag to impregnate in water, water did not had upper soll layer 1cm or so.
1st Wednesday, white careless aerial stem of plant branch germination percentage was 100%, and mean germination number is 3, average leaf number 0;2nd week Mean germination number is 4, average leaf number 10, and average maximum leaf area is 5cm2, average longest stem length is 4cm;3rd week average Germinative number is 4, and average leaf number is 15, and average maximum leaf area is 14cm2, average longest stem length is 7cm;It is 4th week, average Germinative number is 4, and average leaf number is 18, and average maximum leaf area is 30cm2, average longest stem length is 12cm.
Embodiment 4
On September 10th, 2018 acquires the saururus chinensis aerial stem of plant in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank, and rugosity is about 2cm, stem top blade 80% is seriously withered and yellow, and 1/2 or less height stem of branch, leaf are integrally also very healthy and strong, belongs to uneducated person's type on the ground Stem.25~30cm of its base portion or so length stem section is intercepted (i.e. from the stem on the basal part of stem to base portion of ground between 25~30cm or so Section), remove all blades, retains base portion section comparatively dense stem section, in this, as cutting in South China Botanical Garden Chinese Academy of Sciences greenhouse Experiment in cultivation is carried out in greenhouse.Test period is September in 2018 10 days-October 8, totally 4 weeks, routine observation saururus chinensis weekly Growing state, including germination percentage (germination branch number/total branch number), mean germination number (germination sum/total branch number), average leaf The piece number (blade amt/total branch number), average maximum blade area (the sum of maximum leaf area/total branch number on every root cutting) and Average longest shoot length (the sum of longest shoot length/total branch number on every root cutting).September daily mean temperature is 33 DEG C, night Temperature on average is 25 DEG C, when average sunshine a length of 13h.
The horizontal bubble of stem section (cutting) used will be tested in the water of lower part nutritious native (peat soil: pond sludge: sand=5:2:1m/m) In body, 3, every basin, totally 5 basin.First week, the saururus chinensis stipes for swimming in the water surface, which goes out, grew sprouting, germination percentage nearly 50%, also nothing Mounted blade;Second week tests the survival rate of branch up to 100%, and germination percentage is up to 100%, and mean germination number is 3.3, Ye Ping Mean is 6, and average maximum leaf area is 10cm2, average most long shoot (shoot) reaches 4cm.At this point, moving it into Nutrition Soil (mud Charcoal soil: pond sludge: sand=5:2:1m/m) in continue to cultivate, the disinfection way of Nutrition Soil are as follows: with 25% metalaxyl wettable powder 5g adds water 5kg, and Nutrition Soil 100kg is sprayed after mixing, and in spray, mix is uniform, after accumulating 2h, can be transplanted, cultivation side Formula, which is that level is horizontal, buries, and thickness of earth-fill cover about 2cm cultivates spacing about 10cm;It pours a water at dusk daily, guarantees that moisture is sufficient, it should not There is ponding;4th week, mean germination number was 3.8, and average leaf number is 11, and average maximum leaf area is 40cm2, averagely most Long shoot reaches 18cm.To height of seedling 25cm or so, field planting of transplanting seedlings.It is divided into 2-3 sections for stem is disconnected according to shoot position, every segment length 7~ 10cm, notch is not too near from shoot, handles notch with the water-reducible carbendazim of volume ratio 1:1000, avoids wound infection.It will New talent moves into cultivation in planting bag, and planting bag surrounding is pricked hole, is placed in pond, cultivation matrix in bag (peat soil: pond sludge: husky= 5:2:1m/m) high 25cm, nursery stock thickness of earth-fill cover are 8~10cm, the pond depth of water about 10cm.Transplanting first 3 days daily in planting bag A water is poured, a water supplement evaporation capacity is poured within every 2~3 days after transplanting, keeps water depth in the half or so of soil height.
The contrast effect that saururus chinensis water planting moves back the upgrowth situation of the upgrowth situation and vertical cuttage into Nutrition Soil is shown in Fig. 8.
Comparative example 1
On August 25th, 2018 acquires saururus chinensis in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank, and flower has withered at this time Thank, top blade-section is obviously withered and yellow, the saururus chinensis nursery stock for stem stalwartness of choosing, rugosity about 1.5cm, take its base portion 25~ 30cm stem section (i.e. from the stem section on the basal part of stem to base portion of ground between 25~30cm) removes all blades, and it is close to retain bottom section Collect section, carries out water culture experiment.Test water is pig farm breeding wastewater, is contained in blue water tank, every case is white with water 60L, three 15, careless branch, traverse make its free buoyant growth in the water surface.Test period be the 26 days-October 1 of August in 2018, totally 5 weeks, The growing state of routine observation saururus chinensis weekly, including germination percentage (germination branch number/total branch number) and height of seedling.Place is four meetings The city's village Nan Tang illumination, draughty simple greenhouse's greenhouse, 26 DEG C of night temperature on average in canopy, daily mean temperature is 34 DEG C, average Sunshine-duration is 13h.
First week, for the germination percentage of the saururus chinensis aerial stem of plant up to 40%, the section budding of nearly base portion was very fast;Second week, germination percentage reach 100%, 70% section has bud, partial blade expansion, average height of seedling 3.5cm on every branch;Third week, blade are further opened up It opens and grows up, average height of seedling 6cm;4th week, average height of seedling 10cm;5th week, New shoot growth was very vigorous, and height of seedling reaches 15cm, test branch grows a large amount of new roots and bottom save intensive place sprout out it is new it is horizontal walk stem, biomass increases quickly.
Comparative example 2
On August 1st, 2018, in the village Nan Tang, Zhaoqing City of Guangdong Province Sihui City, river bank pulls 3 1.5m × 1m saururus chinensis samples Ground carries out field inspection.At this point, saururus chinensis blossom fall about 1 month, there is slight withered and yellow phenomenon in top blade.Test period is August in 2018 1 day-October 15, upgrowth situation of the every 15 days saururus chinensis of observation in field, including base portion germination rate (base Sprout the total branch number of branch number/test in portion), lateral bud branches out rate (lateral bud sprout the total branch number of branch number/test), average longest Shoot length (the longest shoot length of test branch and the/total branch number of test) and average maximum leaf area (the maximum leaf of branch The total branch number of area/test).1 day-September of August 30 days, 26 DEG C of field night temperature on average, daily mean temperature are 34 DEG C;October day 29 DEG C of temperature on average, night temperature on average is 19 DEG C.August part be averaged sunshine when a length of 13h, September part be averaged sunshine when it is a length of 12.5h, average sunshine time in October are 11.5h.
The saururus chinensis for choosing 1/2 area in sample ground, cuts from nearly base portion 30cm or so height, therefrom randomly chooses 10 works For observation object, tag board, upgrowth situation of the every 15 days saururus chinensis of observation in field are hung up.August part branch base on the 16th Sprouting out is sprouted in portion in soil, and for base portion germination rate up to 30%, lateral bud rate of branching out is 70%, most nearby saves from notch and grows at first Shoot, average longest shoot length are 3cm, and average maximum leaf area is 6cm2;August heavy rain on the 30th, is flooded to saururus chinensis sample Not yet, entire plant is fully immersed in water 2 days, observes after September water on the 1st is retreated, base portion germination rate is 50%, and lateral bud is branched out Rate is 80%, and average longest shoot length is 5cm, and average maximum leaf area is 10cm2;September typhoon on the 16th brings heavy rain, and three is white It is flooded to careless sample, is observed after September water on the 18th is retreated, the survival rate of saururus chinensis branch is 100%, and base portion germination rate is again 60%, lateral bud rate of branching out is 100%, and average longest shoot length is 10cm, and average maximum leaf area is 18cm2;October 3, Average longest shoot length is 14cm, and average maximum leaf area is 30cm2.October 15, average longest shoot length are 20cm, And 70% is that sprout average maximum leaf area be 38cm to side shoot2
The saururus chinensis for choosing 1/2 area in sample ground is without any processing, therefrom randomly chooses 10 and is used as observation object, hangs Upper tag board, upgrowth situation of the every 15 days saururus chinensis of observation in field.It is August 16 days, several in branch base portion soil in sample ground It is sprouted without sprouting, stem top blade is slightly withered and yellow;August heavy rain on the 30th, is flooded not to saururus chinensis sample, entire plant is soaked completely Bubble is observed, stem top blade 80% is obviously withered and yellow, and part has been fallen, branch 1/2 2 days in water after September water on the 1st is retreated Following height component stem, leaf are integrally also very healthy and strong;September typhoon on the 16th brings heavy rain, is flooded again to saururus chinensis sample, in September 18 Day, water was observed after retreating, and stem top blade 90% is withered to be fallen, and 1/3 or less height component stem of branch, leaf are integrally also more healthy and stronger;October 3 Day, blade all withers and falls, the hollow blackening of 90% stem;October 15, on ground, entire plant is rotten dead for saururus chinensis lodging, and three The survival rate of the white grass aerial stem of plant is 0.
Comparative example 3
On August 15th, 2018 acquires saururus chinensis branch (aerial stem of plant) in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank. At this point, or so 1 half a month of saururus chinensis blossom fall, stem top blade is slightly withered and yellow, branch (for selecting rugosity about 1cm more delicate Upper stem), nearly base portion 25cm or so length stem section (i.e. from the stem section on the basal part of stem to base portion of ground between 25cm) is intercepted, institute is removed There is blade, retains base portion section comparatively dense section, cultivated as cutting in South China Botanical Garden Chinese Academy of Sciences greenhouse Test.Test period is August in 2018 15 days-September 12 days, totally 4 weeks, the growing state of routine observation saururus chinensis weekly, including Germination percentage (germination branch number/total branch number), mean germination number (bud quantity summation/total branch number), average leaf number (number of sheets amount Summation/total branch number) and average longest shoot length (the longest shoot length summation of observation branch/total branch number).August day is flat Equal temperature is 33 DEG C, and night temperature on average is 26 DEG C, when average sunshine a length of 13h.
By peat soil: pond sludge: the husky ratio according to weight ratio 5:2:1 mixes, and being placed in diameter is 40cm, the plantation of high 30cm In bag, native depth is 25cm.The vertical cuttage of branch (cutting) used will be tested in cultivation matrix, cuttage depth is long 1/3 of branch, 3, every basin, totally 10 basin;Planting bag is impregnated in water, water did not had upper soll layer 1cm or so.
1st week germination percentage is 75%, and mean germination number is 1, average leaf number 0;2nd week germination percentage is 100%, average Germinative number is 2, average leaf number 4, and average maximum leaf area is 4cm2, average longest stem length is 3cm;3rd week mean germination Number is 2, and average leaf number is 6, and average maximum leaf area is 12cm2, average longest stem length is 6cm;4th week, mean germination Number is 3, and average leaf number is 15, and average maximum leaf area is 30cm2, average longest stem length is 10cm.
Comparative example 4
On September 10th, 2018 acquires saururus chinensis branch (aerial stem of plant) in the Zhaoqing City of Guangdong Province Sihui City village Nan Tang river bank, Rugosity about 2cm, stem top blade 80% is seriously withered and yellow, and 1/2 or less height component stem of branch, leaf are integrally also very healthy and strong, belongs to Uneducated person's type branch.Its base portion 25~30cm length stem section is intercepted (i.e. from the stem on the basal part of stem to base portion of ground between 25~30cm Section), remove all blades, retains upper soll layer section comparatively dense stem section, as cutting in South China Botanical Garden Chinese Academy of Sciences greenhouse Experiment in cultivation is carried out in greenhouse.Test period is September in 2018 10 days-October 8, totally 4 weeks, routine observation saururus chinensis weekly Growing state, including germination percentage (germination branch number/total branch number), mean germination number (germination sum/total branch number), average leaf The piece number (blade amt/total branch number), average maximum blade area (the sum of maximum leaf area/total branch number on every root cutting) and Average longest shoot length (the sum of longest shoot length/total branch number on every root cutting).September daily mean temperature is 33 DEG C, night Temperature on average is 25 DEG C, when average sunshine a length of 13h.
The horizontal bubble of branch (cutting) used will be tested in the water of lower part nutritious native (peat soil: pond sludge: sand=5:2:1m/m) In body, 3, every basin, totally 5 basin.First week, the saururus chinensis stipes for swimming in the water surface, which goes out, grew sprouting, germination percentage nearly 50%, also nothing Mounted blade;Second week, germination percentage is up to 100%, and for branch survival rate up to 100%, mean germination number is 3.3, and leaf average is 6 Piece, average maximum leaf area are 10cm2, average most long shoot reaches 4cm;4th week, mean germination number was 3.5, and average leaf number is 7, average maximum leaf area is 15cm2, average most long shoot reaches 10cm.
The vertical cuttage of branch (cutting) used will be tested in Nutrition Soil (peat soil: pond sludge: sand=5:2:1m/m), every basin 3, totally 5 basin.Cuttage depth is long 1/3 of branch, and cultivation distance is 10cm, pours a water at dusk daily, keeps moisture sufficient.The One week germination percentage is 0;Second week, germination percentage 70%, mean germination number are 1, and average leaf number is 1, average maximum leaf Area is 1.5cm2, average most long shoot reaches 1cm;4th week, germination percentage 90%, mean germination number was 1.5, average leaf number It is 2, average maximum leaf area is 6cm2
Saururus chinensis either vigorous growth period (July in May -) is still it can be seen from above-described embodiment 1 and comparative example 1 Nursery stock withering period (August-September), ground basal part of stem (stem is not hollow) all have very strong sprouting Regenerated energy under water planting environment Power, water planting two weeks, bud ratio was up to 100%, but the eugonic branch germination of the 5-7 month is faster, and the speed of growth of shoot is more Fastly.
It can be seen from above-described embodiment 2 and comparative example 2 when the saururus chinensis aerial stem of plant is also more healthy and stronger, from nearly base portion 30cm Stem is cut in left and right, lateral bud can be promoted to sprout to form shoot, extend the viewing period and picking time of saururus chinensis;And by saururus chinensis By it is wild be transplanted into experimental plot when, cutting with contacting for creeping underground stem can promote the saururus chinensis ground intensive place of basal part of stem section to sprout Sprouting is sent out, Cong Dili sends forth branches;The sprouting for being embedded in soil base portion sprouts slowly than revealing aerial lateral bud, but bud is once grown Growth is rapid.
The resistance to water logging of the saururus chinensis and horizontal cuttage of branch is sent out than vertical cuttage it can be seen from above-described embodiment 3 and comparative example 3 Faster, the yield of bud is higher on every test branch, and growing way is more preferable for bud rate, is conducive to improve seedling propagation efficiency with the method, Reduce Plant technique cost.
Water planting saururus chinensis is in germination percentage, germinative number and the life of shoot it can be seen from above-described embodiment 4 and comparative example 4 Long speed everyway is significantly better than vertical cuttage;Saururus chinensis bough water planting is transplanted into Nutrition Soil to 6~10cm, it is horizontal shallow It is embedded with and is conducive to saururus chinensis growth and later period division propagation.Such method breeding nursery stock improves at least 3 than conventional vertical cuttage efficiency Times, especially wither season in saururus chinensis seedling.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change It also should be regarded as protection scope of the present invention into retouching.

Claims (10)

1. a kind of utilize saururus chinensis aerial stem of plant rapid propagation method, which comprises the following steps:
(1) the saururus chinensis aerial stem of plant is obtained, upper soll layer and the most intensive stem section of air joining place section are retained, intercepts ground basal part of stem The stem section of 25~30cm more than to base portion removes most or all of leaf, obtains cutting;
(2) cutting level is disposed across in fertile water body or Huo Gelan nutrient solution, carries out suspension water planting 2~3 weeks, obtained new Branch;
(3) by the direct cultivating and growing of the shoot of acquisition or by it is horizontal it is horizontal bury in a manner of be transplanted into the Nutrition Soil disinfected in advance into Row cultivation, obtains seedling;
(4) seedling of acquisition is subjected to field planting cultivation.
2. according to claim 1 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that described is fertile Water body be pig farm breeding wastewater or the water body containing Nutrition Soil.
3. according to claim 1 or 2 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that described Nutrition Soil is peat soil: pond sludge: husky by weight the mixture for being 5:2:1.
4. according to claim 1 or 2 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the step Suddenly (3) are to Nutritive cube sterilization processing specifically: with every 25% metalaxyl wettable powder 5g, are watered 5kg, spray nutrition after mixing Native 100kg, in spray, mix is uniform, accumulates 2h.
5. according to claim 1 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the step (3) the horizontal horizontal thickness of earth-fill cover buried is 2cm, and cultivation spacing is 10cm.
6. according to claim 1 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the step (3) it pours a water in cultivation at dusk daily, guarantees that moisture is sufficient, not there is ponding.
7. according to claim 1 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the step (4) it is to the high 25cm of seedling, according to shoot position by stem section sub-cut at 2~3 that the seedling by acquisition, which carries out field planting cultivation, Section, every 7~10cm of segment length handle notch with the diluted carbendazim of volume ratio 1:1000, then transplant in crop field or planting bag It cultivated, be colonized.
8. according to claim 7 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the transplanting It cultivates, be colonized in big Tanaka specifically: selection is had abundant water resources, and the convenient field of irrigation and drainage is as crop field;Before big Tagayasu Den is whole It one week, sprays a wide spectrum and goes out the herbicide of natural disposition;Crop field compartment face is flattened and ditched when Tagayasu Den is whole greatly and sets compartment, compartment face width is 1.5~2m, the furrow width between compartment and compartment is 30~40cm, depth is 15~30cm;Cultivation spacing is 20~30cm, and thickness of earth-fill cover is 8~10cm;Just transplanting the last week respectively pours a water sooner or later toward compartment face, and injects water body into ditch, guarantees that the depth of water is the 1/ of ditch depth 2;Transplanting fills the water in field after a week, so that water-holding capacity and compartment face in ditch is maintained an equal level, makes crop field moisture and soil moisture and rice cultivation Quite.
9. according to claim 7 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that the transplanting It cultivated, be colonized in planting bag specifically: planting bag surrounding is pricked into hole, is placed in pond, the high 25cm of cultivation matrix in bag, Nursery stock thickness of earth-fill cover is 8~10cm, and the pond depth of water is 10cm;Transplanting pours a water daily in first 3 days in planting bag, every after transplanting A water supplement evaporation capacity is poured within 2~3 days, keeps water depth in the half of soil height.
10. according to claim 1 or 2 utilize saururus chinensis aerial stem of plant rapid propagation method, which is characterized in that obtain three The time of the white grass aerial stem of plant is 5~July.
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