CN109168685B

CN109168685B - Cutting propagation method of cymbidium longipedunculatum and cutting propagation substrate of cymbidium longipedunculatum

Info

Publication number: CN109168685B
Application number: CN201810895790.2A
Authority: CN
Inventors: 李凌飞; 陈朋; 杨蕾蕾
Original assignee: SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
Current assignee: SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
Priority date: 2018-08-08
Filing date: 2018-08-08
Publication date: 2021-01-26
Anticipated expiration: 2038-08-08
Also published as: CN109168685A

Abstract

The invention discloses a cutting propagation method of long-stem cymbidium and a cutting propagation substrate of the long-stem cymbidium, wherein the cutting propagation method of the long-stem cymbidium comprises the following steps: cutting branches of the cymbidium longipedunculatum, removing stem nodes with inflorescence growing in the branches, and cutting the branches into cutting slips; cutting the cutting slips into the cutting medium of the moistened long-stem cymbidium; and carrying out cuttage management treatment on the cutting slips which are subjected to cuttage into the cuttage matrix, wherein the cuttage management treatment comprises the step of spraying foliar fertilizer on the cutting slips. The method for cutting propagation of the cymbidium longipedunculatum utilizes branches of the cymbidium longipedunculatum as propagation tissues and adopts a cutting mode for propagation, thereby effectively avoiding the defects of low efficiency and high requirement on propagation conditions of the existing seed and plant division propagation. In addition, the selected cutting slips of the long-stem cymbidium goeringii are cut into the cutting medium of the long-stem cymbidium goeringii for propagation, so that the propagation survival rate is effectively improved, and the stability of the variety can be ensured.

Description

Cutting propagation method of cymbidium longipedunculatum and cutting propagation substrate of cymbidium longipedunculatum

Technical Field

The invention belongs to the technical field of plant tissue propagation, and particularly relates to a method for cutting propagation of cymbidium longipedunculatum and a medium for cutting propagation of the cymbidium longipedunculatum.

Background

The long-stem Crane top blue (Phaius longicrris Z.H.Tsi) is in the shape of a pseudobulb cylinder, is 20-40 cm long and 1.3-2 cm thick, has 3-8 knots, and has a length of 5-7 cm between the knots. The leaves usually have 6 leaves which are grown on the upper part of the pseudobulb, the leaves are ellipse or ellipse and shaped like needles, the scapes usually have 2 leaves, and the length is up to 45 cm after being drawn from the base or the nodes near the base; the inflorescence handle is 5 mm thick, 3-5 sheaths are thinned, and no hair exists; the total inflorescence has a few flowers, the flowers are medium and large, the flowers are open, and sepals and petals are light yellow and green; the petals are hairless, the shape of the inverted ovum is in a needle shape, and the pollen ball is close to the ovum shape triangle; the flowering period is 8-10 months. The long-stem Carpesium Makino is produced in southern Yunnan China (Jinghong, Meng La) and grows under the valley compact forest with the elevation of 1000-1400 meters, is a national II-level protective plant, is a special species in China, has cultivation and has higher gardening value.

The long-stem cymbidium has incomplete seed development like other orchids, does not contain endosperm or other nutritional structures for providing nutrition for seed germination, requires a very long time to obtain flowering plants through seed propagation under natural conditions, and has very low germination rate and survival rate. In addition, due to over-mining and damage to habitat for a long time, wild resources of the long-stem cymbidium sinensis are greatly reduced, the wild resources are in an endangered state at present, and the requirement of people on the cymbidium sinensis cannot be met by depending on the wild resources alone. In recent years, the propagation by means of the separate plant is tried, but the propagation coefficient of the separate plant propagation is very low, the growth speed is very slow, the large-scale propagation in a short time cannot be realized, and the propagation mode leads seedlings to easily carry viruses, causes the degeneration of varieties and severely limits the commercialization of the seedlings.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide a method for cutting propagation of cymbidium longipedunculatum, so as to solve the technical problems of low propagation efficiency and high propagation condition requirement of the current cymbidium pedunculatum.

In order to achieve the above object, according to one aspect of the present invention, there is provided a method for cutting propagation of cymbidium longipedunculatum, comprising the steps of:

cutting a branch of the cymbidium longipedicel, removing a stem node of the branch, which grows an inflorescence, and cutting the branch, from which the stem node of the inflorescence grows, into a cutting shoot;

cutting the cutting slips obtained by cutting into a cutting medium of the long-stem fastidion subjected to the wetting treatment;

and carrying out cuttage management treatment on the cutting slips which are subjected to cuttage into the cuttage matrix, wherein the cuttage management treatment comprises the step of spraying foliar fertilizer on the cutting slips.

In another aspect of the invention, a cutting propagation matrix of the cymbidium longipedunculatum is provided, and comprises one or a mixture of more of garland stone, limestone, absorbent stone, vesuvianite and plant diamond.

Compared with the prior art, the method for cutting propagation of the cymbidium longipedunculatum utilizes branches of the cymbidium pedunculatum as propagation tissues and adopts a cutting mode for propagation, thereby effectively avoiding the defects of low propagation efficiency and high propagation condition requirement in the prior art of adopting seeds and dividing plants. In addition, the selected cutting slips of the long-stem cymbidium goeringii are cut into the cutting medium of the long-stem cymbidium goeringii for propagation, so that the propagation survival rate is effectively improved, and the stability of the variety can be ensured.

The cutting propagation matrix of the cymbidium longipedunculatum adopts specific gravels as raw materials, thereby ensuring the implantation rooting of the cymbidium longipedunculatum cutting slips, improving the survival rate of the cymbidium longipedunculatum cutting slips and ensuring the stability of the variety.

Drawings

Fig. 1 is a process flow of the cutting propagation method of the cymbidium longipedunculatum provided by the embodiment of the invention.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which embodiments of the invention belong. If a definition set forth in this section is contrary to or otherwise inconsistent with a definition set forth in the patents, patent applications, published patent applications, and other publications that are herein incorporated by reference, the definition set forth in this section prevails over the definition that is incorporated herein by reference.

On one hand, the embodiment of the invention provides a method for cutting propagation of cymbidium longipedunculatum, wherein the process flow of the method for cutting propagation of cymbidium longipedunculatum is shown in fig. 1, and the method comprises the following steps:

s01: cutting branches of the cymbidium longipedicel, removing stem nodes growing inflorescences in the branches, and cutting the branches after removing the stem nodes growing inflorescences into cutting slips;

s02: cutting the cutting slips obtained by cutting into the cutting medium of the long-stem cymbidium after being wetted;

s03: and carrying out cuttage management treatment on the cutting slips which are cut into the cuttage matrix, wherein the cuttage management treatment comprises the step of spraying foliar fertilizer on the cutting slips.

In step S01, in order to improve the quality of the cutting, it is preferable that when the cut long-stem cymbidium branches are cut into cutting slips, 1-2-year-old healthy branches of long-stem cymbidium branches are selected, the stem nodes of the healthy branches, from which inflorescences have grown, are removed, and the branches from which the stem nodes of the inflorescences have grown are removed are cut into cutting slips every 1-3 stem nodes, so that the survival rate of the cutting propagation of the cutting slips is ensured to be improved from the propagation tissue source by controlling and selecting the size and quality of the cutting slips.

In step S02, the cutting medium is laid in advance in the bed to form a bed for cutting the cutting wood. In one embodiment, the cutting shoots are inserted into the cutting substrate as follows: after the cutting slips are cut into the cutting medium, the bed surface angle formed by the cutting slips and the cutting medium is 10-30 degrees, and the length of the cutting slips exposed out of the cutting medium is 3-5cm, so that the water and air permeability of the base parts of the cutting slips are ensured, and the base parts of the cutting slips are prevented from being rotten.

Preferably, in another embodiment, the cutting medium comprises one or a mixture of more of boulders, limestone, absorbent stone, vesuvianite, and plant diamond. The substrate can be beneficial to the survival of the cutting slips by cutting, the survival rate is improved, and the cutting slips are prevented from rotting by cutting. Further preferably, the particle size of the broken stone particles is not more than 10mm, so that the cutting slips are in close contact with the cutting matrix, and the humidity around the cutting slips is ensured.

In addition, the cutting medium is laid in the seedling bed, preferably, in one embodiment, the thickness of the cutting medium laid in the seedling bed is 3-15cm, and the thickness can effectively improve the survival rate of the cutting slips.

In step S02, the wetting treatment on the cutting medium may be spraying the cutting medium with water directly or spraying liquid fertilizer that is beneficial for the absorption of the cutting shoot, so that the cutting medium is fully wetted.

In the step S03, the cutting shoot obtained by cutting is inserted into the cutting medium of the moistened orchidaceae longstem, the cutting shoot inserted into the cutting medium is subjected to cutting management treatment, and when the cutting management treatment is performed, foliar fertilizer is sprayed on the cutting shoot to ensure the survival rate of the cutting shoot, so as to finally obtain the cutting shoot seedling. Preferably, in one embodiment, the cutting management treatment comprises spraying foliar fertilizer to the cutting slips every three days, so as to timely and effectively supplement fertilizer to the cutting slips and improve the survival rate of the cutting slips. Preferably, the foliar fertilizer is added with a rooting plant growth regulator to ensure the rooting of the cutting and improve the survival rate of the cutting. Further preferably, the rooting plant growth regulator added in the foliar fertilizer is 0-2mg/L gibberellin, 0-50mg/L naphthylacetic acid or 0-50mg/L indolebutyric acid, so that the rooting of the cutting shoot is ensured, the survival rate of the cutting shoot is further improved, and the propagation efficiency is improved simultaneously by the foliar fertilizer added with the rooting plant growth regulator.

In another embodiment, preferably, when the cutting management treatment is performed on the cutting slips which are cut into the cutting medium, the control of the cutting environment of the cutting slips is further included, so that the spraying of foliar fertilizer is matched, the synergistic effect of the two conditions is realized, and the survival rate and the growth rate of the cutting slips are further improved. Further preferably, when the cutting environment of the cutting shoot is controlled, the shading degree in the propagation environment of the cutting shoot is controlled to be 60-80%, specifically 60%, and the humidity is controlled to be 80-90%, specifically 85%.

And (3) carrying out cuttage management treatment on the cuttage cuttings for 30-45 days, and then starting the growth of the shoot buds of the cuttings. In a further embodiment, after the cutting management treatment is performed on the cutting slips, transplanting the surviving cutting slips, namely the cutting slip seedlings. Specifically, the cutting seedlings are transplanted after the cutting shoots grow to 3-5 cm. As in the specific embodiment, the cuttings are taken out from the seedbed, transplanted into a three-in-one culture medium of bark, turfy soil and river sand, and potted and cultured.

Therefore, the method for the cutting propagation of the cymbidium longipedunculatum utilizes the branch of the cymbidium longipedunculatum as the propagation tissue, adopts the cutting mode for propagation, and effectively avoids the defects of the existing propagation of the cymbidium longipedunculatum. In addition, the selected cutting slips of the long-stem cymbidium goeringii are cut into the cutting medium of the long-stem cymbidium goeringii for propagation, so that the propagation survival rate is effectively improved, and the stability of the variety can be ensured.

On the other hand, the embodiment of the invention also provides a cutting propagation matrix of the cymbidium longipedunculatum, and the cutting propagation matrix of the cymbidium longipedunculatum comprises one or a mixture of more of garland stone, limestone, absorbent stone, vesuvianite and plant diamond.

When the cutting propagation matrix of the long-stem fastidium is a mixture of a plurality of gravels, the cutting propagation matrix of the long-stem fastidium can be a mixture with the following volume ratio:

in one embodiment, the cutting propagation substrate of the cymbidium longipedunculatum is orchids.

In another embodiment, the cutting propagation substrate of the cymbidium longipedunculatum comprises limestone: mixture of vesuvianite 5: 1. Wherein, preferably, the particle size of the crushed stone particles is not more than 10 mm. Thus, the cutting propagation matrix of the cymbidium longipedunculatum adopts specific gravels as raw materials, thereby ensuring the implantation rooting of the cutting slips of the cymbidium longipedunculatum, improving the survival rate of the cutting slips of the cymbidium longipedunculatum and ensuring the stability of the variety.

The present invention will now be described in further detail with reference to a specific method for cutting propagation of cymbidium longipedunculatum.

Example 1

The embodiment provides a method for cutting propagation of cymbidium longipedunculatum, which comprises the following steps:

s11, preparing a cutting material:

selecting 1-year-old healthy branches of the long-stem cymbidium, removing stem nodes of the selected branches after inflorescence growth, and cutting the selected branches into cuttings for later use according to 2 stem nodes;

s12, laying of a cuttage matrix and a seedbed:

selecting orchid macadam with the particle size not larger than 10mm, and paving the macadam into a seedbed with the thickness of 10 cm;

s13, cuttage and induction:

and (4) after the seedbed laid in the step (S12) is fully wetted by spraying water, obliquely inserting the cutting slips in the step (S11) into the substrate at an angle of 20 degrees between the cutting slips and the surface of the seedbed, wherein the exposed substrate of each cutting slip is 4 cm. Spraying foliar fertilizer added with Gibberellin (GA)1mg/L and naphthylacetic acid (NAA)30mg/L once every three days, wherein the stem buds begin to grow after 40 days of sprouting, and the seedbed is laid in a constant environment with shading degree of 60% and humidity of 85%; the germination rate is 87% and no rot exists.

S14, transplanting:

and (5) growing the cutting seedlings formed in the step (S13) to the height of 3-5cm, taking out the cutting seedlings from the seedbed, transplanting the cutting seedlings into a tree bark, turfy soil and river sand three-in-one culture medium, and potting and culturing.

Example 2

s21, preparing a cutting material:

selecting a 2-year-old healthy branch of the cymbidium longisimum, removing the stem nodes of the selected branch after inflorescence growth, and cutting the selected branch into cuttings for later use according to 2-3 stem nodes;

s22, laying of a cuttage matrix and a seedbed:

selecting limestone with the particle size not larger than 10 mm: paving the vesuvianite into a seedbed with the thickness of 5cm according to mixed crushed stones with the volume ratio of 5: 1;

s23, cuttage and induction:

and (4) after the seedbed laid in the step (S22) is fully wetted by spraying water, inserting the cutting slips in the step (S21) into the substrate in an inclined mode at an angle of 30 degrees between the cutting slips and the surface of the seedbed, and exposing the substrate of each cutting slip for 4-5 cm. Spraying foliar fertilizer added with 2mg/L of Gibberellin (GA) and 10mg/L of naphthylacetic acid (NAA) once every three days, wherein 30 days of stem bud germination starts to grow, and a seedbed is laid in a constant environment with 70% of shading degree and 80% of humidity; the bud ratio is 95 percent, and no rot exists.

S24, transplanting:

and (5) growing the cutting seedlings formed in the step (S23) to the height of 3-5cm, taking out the cutting seedlings from the seedbed, transplanting the cutting seedlings into a tree bark, turfy soil and river sand three-in-one culture medium, and potting and culturing.

Example 3

s31, preparing a cutting material:

selecting a 2-year-old healthy branch of the cymbidium longisimum, removing the stem nodes of the selected branch after inflorescence growth, and cutting the selected branch into cuttings for later use according to 1-3 stem nodes;

s32, laying of a cuttage matrix and a seedbed:

selecting the plant diamond with the grain diameter not more than 10 mm: limestone is mixed and paved into a seedbed with the thickness of 10cm according to the volume ratio of 1: 1;

s33, cuttage and induction:

and (4) after the seedbed laid in the step (S32) is fully wetted by spraying water, inserting the cutting slips in the step (S21) into the substrate in an inclined mode at an angle of 10 degrees of the cutting slips and the surface of the seedbed, and exposing the substrate of each cutting slip for 4-5 cm. Spraying foliar fertilizer added with Gibberellin (GA)1.5mg/L and naphthylacetic acid (NAA)30mg/L once every three days, wherein 30 days of stem bud germination starts to grow, and a seedbed is laid in a constant environment with shading degree of 80% and humidity of 80%; the bud ratio is 84%, and no rot exists.

S34, transplanting:

and (5) growing the cutting seedlings formed in the step (S33) to the height of 3-5cm, taking out the cutting seedlings from the seedbed, transplanting the cutting seedlings into a tree bark, turfy soil and river sand three-in-one culture medium, and potting and culturing.

Comparative example 1

s11, preparing a cutting material:

s12, laying of a cuttage matrix and a seedbed:

selecting limestone with the particle size not larger than 10 mm: the peat soil is mixed and paved into a seedbed with the thickness of 10cm according to the volume ratio of 1: 1;

s13, cuttage and induction:

and (4) after the seedbed laid in the step (S22) is fully wetted by spraying water, inserting the cutting slips in the step (S21) into the substrate in an inclined mode at an angle of 30 degrees between the cutting slips and the surface of the seedbed, and exposing the substrate of each cutting slip for 4-5 cm. Spraying foliar fertilizer added with 2mg/L of Gibberellin (GA) and 50mg/L of naphthylacetic acid (NAA) once every three days, wherein 30 days of stem bud germination starts to grow, and a seedbed is laid in a constant environment with shading degree of 60% and humidity of 85%; the germination rate is 34 percent, and the rottenness is 52 percent.

S14, transplanting:

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. A cutting propagation method of the cymbidium longipedicel is characterized by comprising the following steps:

2. The method according to claim 1, wherein the step of cutting a shoot of the long-stem cymbidium goeringii, removing a stem node of the shoot from which an inflorescence has grown, and cutting the shoot from which the stem node of the inflorescence has grown into cuttings comprises:

selecting 1-2 year-old healthy branches of the cymbidium longipedunculatum, removing the stem nodes with inflorescences grown in the healthy branches, and cutting the branches with the stem nodes with the inflorescences removed into the cuttings according to 1-3 stem nodes.

3. The method according to any one of claims 1-2, wherein the cutting substrate comprises one or a mixture of several of the group consisting of boulders, limestone, absorbent stones, vesuvianite, and plant diamond.

4. The method according to claim 3, characterized in that the crushed stone particles have a particle size not greater than 10mm and/or the cutting substrate is laid to a thickness of 3-15 cm.

5. The method according to any one of claims 1-2, wherein the step of cutting the cuttings obtained by cutting into the cutting medium of the wet-treated cymbidium longipedicel comprises:

and cutting the cutting slips obtained by cutting into a cutting medium of the long-stem fastidium after the wetting treatment, so that the bed surface angle of the seedbed formed by the cutting slips and the cutting medium is 10-30 degrees.

6. The method according to any one of claims 1-2, wherein the step of subjecting the cutting shoots that have been cut into the cutting substrate to a cutting management treatment comprises:

and spraying foliar fertilizer to the cutting slips every three days.

7. The method of any one of claims 1-2, wherein the foliar fertiliser comprises 0-2mg/L gibberellin, 0-50mg/L naphthylacetic acid or indolebutyric acid.

8. The method according to any one of claims 1-2 and 4, wherein the step of performing a cutting management treatment on the cutting slips cut into the cutting medium further comprises:

and controlling the shading degree of the cutting in the propagation environment to be 60-80% and the humidity to be 80-90%.