CN109168421A - Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain - Google Patents
Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain Download PDFInfo
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- CN109168421A CN109168421A CN201811175636.4A CN201811175636A CN109168421A CN 109168421 A CN109168421 A CN 109168421A CN 201811175636 A CN201811175636 A CN 201811175636A CN 109168421 A CN109168421 A CN 109168421A
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- inflorescence
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention belongs to plant breeding method technical fields, and in particular to a kind of application of brassicacarinata in the breeding that cabbage type rape definite inflorescence keeps system, sterile line, restorer and hybrid.The cabbage type rape definite inflorescence keeps system with brassicacarinata for female parent, using cabbage type rape indefinite inflorescence as male parent, hybridization, obtain hybrid generation, it is maternal as non-circulation, using cabbage type rape indefinite inflorescence as circulation male parent, cabbage type rape definite inflorescence is obtained after continuous backcross, selfing and keeps system, and is applied in cabbage type rape definite inflorescence sterile line, cabbage type rape definite inflorescence restorer and the breeding of definite inflorescence hybrid.Cabbage type rape definite inflorescence strain provided by the invention solves the problems, such as that indefinite inflorescence increases Oilseed rape biomass effective consumption, reduce mechanical loss rate, improve main shaft and branch silique seed mass of 1000 kernel, single plant yield and yield, lodging rate significantly reduces simultaneously, provides new varieties for rape entire mechanization operation.
Description
Technical field
The invention belongs to plant breeding method technical fields, and in particular to a kind of brassicacarinata has in cabbage type rape
Limit the application in the breeding of inflorescence holding system, sterile line, restorer and hybrid.
Background technique
Rape is the important oil crops in China, currently, normal rapeseed kind is indefinite inflorescence, the florescence is longer, on the one hand
So that the silique maturation time of rapeseed plants different levels is inconsistent, usually there are the mature flavescence of lower part silique, upper corners
Fruit still prematurity the phenomenon that；Too early, top silique prematurity influences yield composition to harvest；Harvest too late, easily open by lower part silique
It splits, mechanical harvest loss is big, has seriously affected rape production mechanization harvest；On the other hand, in the growth of rape later period, indefinite inflorescence is normal
Cause at the top of inflorescence silique can not normal development and form invalid inflorescence, there is abortion inflorescence or to no effect axis phenomenon, increase
The effective consumption of Oilseed rape biomass reduces the performance of seed yield per plant potentiality.Therefore, lack the product for being suitble to do mechanization operation
Kind is the bottleneck for restricting rape production mechanization production, and breeding is suitble to the new rape variety of do mechanization operation to cause breeder
Great attention, will also be increasingly becoming one of the important directions of rapeseed breeding.
Rape hybrid often is prepared using sterile line in rape seed, production of hybrid seeds process is can simplify, reduces the life of hybrid seed
Cost is produced, hybrid quality and purity are improved, hybrid is made to have advantage by force and well adapting to property, expand hybrid vigour utilizes model
It encloses；Restorer Pistil And Stamen is normal, energy self-fertility, and its pollen can be invested to sterile line and bear seeds the plant grown up to, and
Keep its fertility restorer normal, and there is apparent hybrid vigour in the characters such as yield；Keeping system is that sterile line is rely passage
Essential male parent line.Rape keeps system, sterile line and restorer triple crossing or sterile line and restorer hybridization, can
Realize and have complementary advantages have many advantages, such as that strong excellent combination, hybrid vigour is strong, yield is high, degeneration-resistant strong, the production of hybrid seeds is easy, and is widely used
In large-scale production.
When definite inflorescence is that the generation of plant strain growth and inflorescence is reached to a certain degree, inflorescence meristem be converted to spend it is mitogenetic
Tissue plant and inflorescence top form cluster or a terminal flower, thus the phenomenon that being automatically stopped growth.Rape definite inflorescence can
Using as a very prominent and stable phenotype marker character by its transformation into rape variety, pass through definite inflorescence material
Initiative have become one of the effective way for overcoming the problems, such as that indefinite inflorescence is brought, but it is few by definite inflorescence character transformation
To the research in rape ternary hybrid.
Summary of the invention
Therefore, the technical problem to be solved in the present invention is that existing rape variety is overcome to be not suitable for do mechanization operation, oil
Dish Later growth forms the problems such as indefinite inflorescence increases Oilseed rape biomass effective consumption, exists to provide a kind of brassicacarinata
Cabbage type rape definite inflorescence keeps the application in the breeding of system, sterile line, restorer and hybrid.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
The present invention provides a kind of brassicacarinatas (angstrom mustard) in the breeding that cabbage type rape definite inflorescence keeps system
Application.
Using cabbage type rape indefinite inflorescence as male parent, it is female parent with angstrom mustard, is hybridized, obtain hybrid generation F1；
It is that non-circulation is maternal with the hybrid generation F1, using the cabbage type rape indefinite inflorescence strain as male parent, continuously
Backcrossing obtains cabbage type rape definite inflorescence and keeps system after selfing；
Wherein, the non-circulation female parent is the plant for showing as definite inflorescence character；
The backcrossing number is 4-5 times；
The selfing number is 1 time.
In the Breeding Process, controlling daily light application time is 14h-16h.
In the Breeding Process, Dressing is carried out to Ethiopia's canola seed using potassium permanganate solution；
The mass fraction of the potassium permanganate is 0.1%-0.5%.
The present invention provides a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line.
The cabbage type rape definite inflorescence obtained with breeding in above-mentioned application keeps system for male parent, with cabbage type rape backbone
Sterile line is female parent, is hybridized, obtains F2；
It is female parent with F2, keeps system for male parent with the cabbage type rape definite inflorescence, carry out first time backcrossing, returned
Hand over offspring BC1；
It is that non-circulation is maternal with BC1, keeps system for circulation male parent with the cabbage type rape definite inflorescence, continuous backcross,
Obtain cabbage type rape definite inflorescence sterile line；
Wherein, the F2 is the plant for showing as definite inflorescence character and sterile character；
The BC1 is the plant for showing as definite inflorescence character and sterile character；
The number of the continuous backcross is 4-5 times.
The present invention provides a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer.
System is kept to make with the cabbage type rape definite inflorescence that breeding in above-mentioned application obtains maternal, it is unlimited with cabbage type rape
Inflorescence elite restorer lines make male parent, and hybridization obtains hybrid generation F3；F3 obtains F4 after being selfed 1 time；
It is that non-circulation is maternal with the F4；Cabbage type rape indefinite inflorescence elite restorer lines are circulation male parent；It carries out the 1st time
Backcrossing, obtains backcross progeny；
It is that non-circulation is maternal with the backcross progeny；Circulation father is reverted to the cabbage type rape indefinite inflorescence backbone
This, continuous backcross, selfing obtains cabbage type rape definite inflorescence restorer；
Wherein, it is khaki plant that the F3, which is pattern,；
The nonrecurrent parent is definite inflorescence character and pattern is khaki plant；
The backcrossing number is 4 or 5 times, and the selfing number is 2 times.
The present invention provides a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence hybrid.
The cabbage type rape definite inflorescence obtained with breeding in above-mentioned application keeps system for male parent, with breeding in above-mentioned application
Obtained cabbage type rape definite inflorescence sterile line is female parent, and hybridization obtains cabbage type rape definite inflorescence sterile line parent；
It is female parent, the Wild cabbage type obtained with breeding in above-mentioned application with the cabbage type rape definite inflorescence sterile line parent
Rape definite inflorescence restorer is male parent, and hybridization obtains definite inflorescence hybrid.
Technical solution of the present invention has the advantages that
1. a kind of brassicacarinata provided by the invention answering in the breeding that cabbage type rape definite inflorescence keeps system
With, for the first time by brassicacarinata be used for cabbage type rape definite inflorescence keep system Breeding Process in, selection be with angstrom
Mustard is female parent, and using cabbage type rape indefinite inflorescence strain as male parent, hybridization obtains hybrid generation；With hybrid generation for non-circulation
Female parent, using cabbage type rape indefinite inflorescence strain as circulation male parent, continuous backcross obtains cabbage type rape definite inflorescence after selfing
Keep system.The application into cabbage type rape indefinite inflorescence strain, successfully selects definite inflorescence character transformation in angstrom mustard sweet
Blue type rape definite inflorescence is kept, and the inflorescence top of stem and branch develops into a flower and terminates indefinite inflorescence, transformation
As definite inflorescence, it is automatically stopped growth, solves the problems, such as that indefinite inflorescence increases Oilseed rape biomass effective consumption；With unlimited flower
Sequence strain is compared, which is kept for the florescence of system can at most shorten 14 days, and silique maturity is more consistent, machine
Tool loss late can at most reduce by 3.9%, and main shaft and branch silique seed mass of 1000 kernel and single plant yield significantly improve, and be that rape is whole
Do mechanization operation provides new varieties.
2. a kind of brassicacarinata answering in the breeding of cabbage type rape definite inflorescence sterile line provided by the invention
With, brassicacarinata is used for for the first time in the Breeding Process of cabbage type rape definite inflorescence sterile line, selection be with sweet
It is male parent that blue type rape definite inflorescence, which keeps system, is female parent with Wild cabbage type backbone sterile line, and hybridization obtains hybrid generation F2, with
F2 is that non-circulation is maternal, keeps system for non-circulation male parent with the cabbage type rape definite inflorescence, continuous backcross obtains Wild cabbage type
Rape definite inflorescence sterile line.The sterile rate of the cabbage type rape definite inflorescence sterile line is 100%, with indefinite inflorescence strain phase
Than the florescence of the cabbage type rape definite inflorescence sterile line can at most shorten 14 days, and silique maturity is more consistent, mechanical loss rate
4.4% can be at most reduced, main shaft and branch silique seed mass of 1000 kernel and single plant yield are improved.
3. a kind of brassicacarinata answering in the breeding of cabbage type rape definite inflorescence restorer provided by the invention
With selection is to keep system to make female parent with cabbage type rape definite inflorescence, with cabbage type rape indefinite inflorescence elite restorer lines
Make male parent, hybridizes, obtain hybrid generation F3；F3 obtains F4 after being selfed 1 time；Be that non-circulation is maternal with F4, with cabbage type rape without
Limit inflorescence elite restorer lines are circulation male parent, and continuous backcross is selfed, obtains cabbage type rape definite inflorescence restorer.The wild cabbage
The recovery rate of type rape definite inflorescence restorer is 100%；Compared with indefinite inflorescence strain, the cabbage type rape definite inflorescence is extensive
The florescence for being again can at most shorten 15 days, and silique maturity is more consistent, and mechanical loss rate can at most reduce by 3.3%, main shaft and point
Branch silique seed mass of 1000 kernel and single plant yield are improved.
4. a kind of application of the brassicacarinata provided by the invention in the breeding of cabbage type rape definite inflorescence hybrid,
Selection is to keep system for male parent with cabbage type rape definite inflorescence, is female parent with cabbage type rape definite inflorescence sterile line,
Hybridization, obtains cabbage type rape definite inflorescence sterile line parent；It is female parent with cabbage type rape definite inflorescence sterile line parent, with
Cabbage type rape definite inflorescence restorer is male parent, and hybridization obtains definite inflorescence hybrid.The purity of the definite inflorescence hybrid exists
95% or more；Compared with indefinite inflorescence strain, the florescence of the definite inflorescence hybrid can at most shorten 14 days, and silique maturity is compared with one
It causes, mechanical loss rate can at most reduce by 5.9%, and main shaft and branch silique seed mass of 1000 kernel and single plant yield are improved, lodging
Rate significantly reduces, and promotes and applies for Rape-seed production and provides a kind of novel cabbage type rape definite inflorescence seed.
5. brassicacarinata provided by the invention cabbage type rape definite inflorescence keep system, sterile line, restorer and
Application in the breeding of hybrid, by the florescence for controlling the adjustable cabbage type rape of light application time in Breeding Process；It is planting
Dressing is carried out to brassicacarinata by using liquor potassic permanganate before planting, cabbage type rape definite inflorescence can be improved
The germination percentage of strain.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 be in embodiment 1 cabbage type rape definite inflorescence keep be hybrid generation F1 reductional cell division dyeing
Body behavior, wherein A is F1 chromosome number；B is reductional cell division diakinesis chromosome behavior；C is I in reductional cell division
Phase chromosome behavior；D is I phase chromosome behavior after reductional cell division；E is I phase chromosome behavior after reductional cell division；F
It is II phase chromosome behavior in reductional cell division；G is II phase chromosome behavior after reductional cell division；
Fig. 2 is the dyeing of the reductional cell division of cabbage type rape definite inflorescence sterile line backcross progeny BC1 in embodiment 1
Body behavior, wherein A is BC1 chromosome number；B is reductional cell division diakinesis chromosome behavior；C is I in reductional cell division
Phase chromosome behavior；D is I phase chromosome behavior after reductional cell division；E is I phase chromosome behavior after reductional cell division；F
It is II phase chromosome behavior after reductional cell division.
Specific embodiment
There is provided following embodiments is to preferably further understand the present invention, it is not limited to the best embodiment party
Formula is not construed as limiting the contents of the present invention and protection scope, anyone under the inspiration of the present invention or by the present invention and its
The feature of his prior art is combined and any and identical or similar product of the present invention for obtaining, all falls within of the invention
Within protection scope.
Specific experiment step or condition person are not specified in embodiment, according to the literature in the art described routine experiment
The operation of step or condition can carry out.Reagents or instruments used without specified manufacturer, being can be by commercially available acquisition
Conventional reagent product.
Embodiment 1
All Breeding Process of the present embodiment carry out under field conditions (factors)；
Present embodiments provide a kind of brassicacarinata answering in the breeding that cabbage type rape definite inflorescence keeps system
With selection specifically:
In March, 2010 with 1 part of angstrom of mustard is female parent in Nanchang, with 1 part of cabbage type rape indefinite inflorescence strain (in it is double
11) it is male parent, is hybridized, therefrom screens the plant of definite inflorescence character, obtain 1 part of hybrid generation F1；
With 1 part of hybrid generation F1 it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out first in July, 2010
Secondary backcrossing obtains 5 parts of backcross progeny；
With 5 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out second in March, 2011
Backcrossing, obtains 10 parts of backcross progeny；
With 10 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out third time in July, 2011
Backcrossing, obtains 30 parts of backcross progeny；
With 30 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out the 4th time in March, 2012
Backcrossing, obtains 50 parts of backcross progeny；
With 50 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out the 5th time in July, 2012
Backcrossing, was then selfed for 1 generation, obtains 80 parts of cabbage type rape definite inflorescences and keeps system.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line
Using selection specifically:
In March, 2013, Nanchang keep system for male parent, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish backbone sterile line (RF02-004) is female parent, is hybridized, obtains 1 part of filial generation F2；
In July, 2013, Xining, Qinghai are that non-circulation is maternal with 1 part of F2, keep system with 1 part of cabbage type rape definite inflorescence
For circulation male parent, first time backcrossing is carried out, 20 parts of backcross progeny BC1 are obtained；
Female parent is made with 20 parts of BC1 in March, 2014, Nanchang, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
It carries out second to be returned, obtains 50 parts of backcross progeny BC2；
In July, 2014, Xining, Qinghai are female parent with 50 parts of BC2, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
Third time backcrossing is carried out, 100 parts of backcross progeny BC3 are obtained；
In March, 2015, Nanchang is female parent with 100 parts of BC3, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This progress the 4th time backcrossing, obtains 100 parts of backcross progeny BC4；
In July, 2015, Xining, Qinghai is female parent with 100 parts of BC4, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This, carries out the 5th backcrossing, obtains 100 parts of cabbage type rape definite inflorescence sterile lines.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer
Using selection specifically:
In March, 2013, Nanchang keep system to make maternal, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish indefinite inflorescence elite restorer lines (C01) make male parent, and hybridization obtains 1 part of hybrid generation F3；
In July, 2013, Nanchang are selfed to obtain 1 part of F4 with 1 part of F3；
In March, 2014, Xining, Qinghai are that non-circulation is maternal with 1 part of F4, using 1 part of C01 as circulation male parent, carry out the 1st time and return
It hands over, obtains 10 parts of backcross progenies；
In July, 2014, Nanchang is female parent with 10 parts of backcross progenies, makees male parent with 1 part of C01, carries out second time
It hands over, obtains 20 parts of backcross progenies；
In March, 2015, Xining, Qinghai is female parent with 20 parts of backcross progenies, makees male parent with 1 part of C01, carries out third time and returns
It hands over, obtains 50 parts of backcross progenies；
In July, 2015, Nanchang is female parent with 50 parts of backcross progenies, makees male parent with 1 part of C01, carries out the 4th time and returns
It hands over, obtains 100 parts of backcross progenies；
In March, 2016, Xining, Qinghai, backcross progeny are continuously selfed after 2 generations that obtain 100 parts of cabbage type rape definite inflorescences extensive
Multiple system.
The present embodiment additionally provides a kind of brassicacarinata and applies in the breeding of cabbage type rape definite inflorescence hybrid,
Selection specifically:
In July, 2016 keeps system for male parent, with 1 part of Wild cabbage type in Xining, Qinghai with 1 part of cabbage type rape definite inflorescence
Rape definite inflorescence sterile line is female parent, and hybridization obtains 10 parts of cabbage type rape definite inflorescence sterile line parents；In March, 2017,
It is female parent with 10 parts of cabbage type rape definite inflorescence sterile line parents in Nanchang, it is extensive with 1 part of cabbage type rape definite inflorescence
Multiple system is male parent, and hybridization obtains 50 parts of definite inflorescence hybrids.
It is observed that Fig. 1 be cabbage type rape definite inflorescence keep be hybrid generation F1 reductional cell division chromosome
Behavior, available from A figure, chromosome number, 2n=36；Observe that univalent, divalent occurs in diakinesis pairing in B figure
Body, trivalent, quadrivalent；Observe that I phase chromosome occurred and falls behind behavior in C figure；I phase separation after being observed in D figure, 17:19 points
From；There is the backward behavior of chromosome in I phase after observing in E figure；There is the backward behavior of chromosome in II phase in observing in F figure；G figure
There is the backward behavior of chromosome in II phase after observing.
Fig. 2 is the chromosome behavior of the reductional cell division of cabbage type rape definite inflorescence sterile line backcross progeny BC1, from
Available, chromosome number, 2n=38 in A figure；Observe that the diakinesis 19 matches bivalent in B figure；In being observed in C figure
I phase chromosome does not fall behind；There is 19:19 in I phase after observing in D figure, chromosome does not occur and falls behind；It is observed in E figure
There is 18:20 separation, chromosome does not occur and fall behind；Observe that last II phase separation is normal in F figure, dye-free body falls behind behavior.
Embodiment 2
In the Breeding Process of the present embodiment, the daily light application time of plant is 15h, when illumination deficiency, is carried out using light
Irradiation, control intensity of light are identical as natural light；
Present embodiments provide a kind of brassicacarinata answering in the breeding that cabbage type rape definite inflorescence keeps system
With selection specifically:
In March, 2010 with 1 part of angstrom of mustard is female parent in Nanchang, with 1 part of cabbage type rape indefinite inflorescence strain (in it is double
11) it is male parent, is hybridized, therefrom screens the plant of definite inflorescence character, obtain 1 part of hybrid generation F1；
With 1 part of hybrid generation F1 it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out first in July, 2010
Secondary backcrossing obtains 5 parts of backcross progeny；
With 5 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out second in March, 2011
Backcrossing, obtains 10 parts of backcross progeny；
With 10 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out third time in July, 2011
Backcrossing, obtains 30 parts of backcross progeny；
With 30 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out the 4th time in March, 2012
Backcrossing, obtains 50 parts of backcross progeny；
With 50 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out the 5th time in July, 2012
Backcrossing, was then selfed for 1 generation, obtains 100 parts of cabbage type rape definite inflorescences and keeps system.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line
Using selection specifically:
In March, 2013, Nanchang keep system for male parent, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish backbone sterile line (RF02-004) is female parent, is hybridized, obtains 1 part of filial generation F2；
In July, 2013, Xining, Qinghai are that non-circulation is maternal with 1 part of F2, keep system with 1 part of cabbage type rape definite inflorescence
For circulation male parent, first time backcrossing is carried out, 20 parts of backcross progeny BC1 are obtained；
Female parent is made with 20 parts of BC1 in March, 2014, Nanchang, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
It carries out second to be returned, obtains 50 parts of backcross progeny BC2；
In July, 2014, Xining, Qinghai are female parent with 50 parts of BC2, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
Third time backcrossing is carried out, 100 parts of backcross progeny BC3 are obtained；
In March, 2015, Nanchang is female parent with 100 parts of BC3, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This progress the 4th time backcrossing, obtains 100 parts of backcross progeny BC4；
In July, 2015, Xining, Qinghai is female parent with 100 parts of BC4, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This, carries out the 5th backcrossing, obtains 100 parts of cabbage type rape definite inflorescence sterile lines.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer
Using selection specifically:
In March, 2013, Nanchang keep system to make maternal, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish indefinite inflorescence elite restorer lines (C01) make male parent, and hybridization obtains 1 part of hybrid generation F3；
In July, 2013, Nanchang are selfed to obtain 1 part of F4 with 1 part of F3；
In March, 2014, Xining, Qinghai are that non-circulation is maternal with 1 part of F4, using 1 part of C01 as circulation male parent, carry out the 1st time and return
It hands over, obtains 10 parts of backcross progenies；
In July, 2014, Nanchang is female parent with 10 parts of backcross progenies, makees male parent with 1 part of C01, carries out second time
It hands over, obtains 20 parts of backcross progenies；
In March, 2015, Xining, Qinghai is female parent with 20 parts of backcross progenies, makees male parent with 1 part of C01, carries out third time and returns
It hands over, obtains 50 parts of backcross progenies；
In July, 2015, Nanchang is female parent with 50 parts of backcross progenies, makees male parent with 1 part of C01, carries out the 4th time and returns
It hands over, obtains 100 parts of backcross progenies；
In March, 2016, Xining, Qinghai, backcross progeny are continuously selfed after 2 generations that obtain 100 parts of cabbage type rape definite inflorescences extensive
Multiple system.
The present embodiment additionally provides a kind of brassicacarinata and applies in the breeding of cabbage type rape definite inflorescence hybrid,
Selection specifically:
In July, 2016 keeps system for male parent, with 1 part of Wild cabbage type in Xining, Qinghai with 1 part of cabbage type rape definite inflorescence
Rape definite inflorescence sterile line is female parent, and hybridization obtains 10 parts of cabbage type rape definite inflorescence sterile line parents；In March, 2017,
It is female parent with 10 parts of cabbage type rape definite inflorescence sterile line parents in Nanchang, it is extensive with 1 part of cabbage type rape definite inflorescence
Multiple system is male parent, and hybridization obtains 50 parts of definite inflorescence hybrids.
Embodiment 3
All Breeding Process of the present embodiment carry out under field conditions (factors)；
Present embodiments provide a kind of brassicacarinata answering in the breeding that cabbage type rape definite inflorescence keeps system
With, before planting, using mass fraction be 0.5% potassium permanganate solution Ethiopia's canola seed is impregnated, 6h
After dry, selection specifically:
In March, 2010 with 1 part of angstrom of mustard is female parent in Nanchang, with 1 part of cabbage type rape indefinite inflorescence strain (in it is double
11) it is male parent, is hybridized, therefrom screens the plant of definite inflorescence character, obtain 1 part of hybrid generation F1；
With 1 part of hybrid generation F1 it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out first in July, 2010
Secondary backcrossing obtains 5 parts of backcross progeny；
With 5 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out second in March, 2011
Backcrossing, obtains 10 parts of backcross progeny；
With 10 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out third time in July, 2011
Backcrossing, obtains 30 parts of backcross progeny；
With 30 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out the 4th time in March, 2012
Backcrossing, obtains 50 parts of backcross progeny；
With 50 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out the 5th time in July, 2012
Backcrossing, was then selfed for 1 generation, obtains 100 parts of cabbage type rape definite inflorescences and keeps system.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line
Using selection specifically:
In March, 2013, Nanchang keep system for male parent, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish backbone sterile line (RF02-004) is female parent, is hybridized, obtains 1 part of filial generation F2；
In July, 2013, Xining, Qinghai are that non-circulation is maternal with 1 part of F2, keep system with 1 part of cabbage type rape definite inflorescence
For circulation male parent, first time backcrossing is carried out, 20 parts of backcross progeny BC1 are obtained；
Female parent is made with 20 parts of BC1 in March, 2014, Nanchang, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
It carries out second to be returned, obtains 50 parts of backcross progeny BC2；
In July, 2014, Xining, Qinghai are female parent with 50 parts of BC2, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
Third time backcrossing is carried out, 100 parts of backcross progeny BC3 are obtained；
In March, 2015, Nanchang is female parent with 100 parts of BC3, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This progress the 4th time backcrossing, obtains 100 parts of backcross progeny BC4；
In July, 2015, Xining, Qinghai is female parent with 100 parts of BC4, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This, carries out the 5th backcrossing, obtains 100 parts of cabbage type rape definite inflorescence sterile lines.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer
Using selection specifically:
In March, 2013, Nanchang keep system to make maternal, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish indefinite inflorescence elite restorer lines (C01) make male parent, and hybridization obtains 1 part of hybrid generation F3；
In July, 2013, Nanchang are selfed to obtain 1 part of F4 with 1 part of F3；
In March, 2014, Xining, Qinghai are that non-circulation is maternal with 1 part of F4, using 1 part of C01 as circulation male parent, carry out the 1st time and return
It hands over, obtains 10 parts of backcross progenies；
In July, 2014, Nanchang is female parent with 10 parts of backcross progenies, makees male parent with 1 part of C01, carries out second time
It hands over, obtains 20 parts of backcross progenies；
In March, 2015, Xining, Qinghai is female parent with 20 parts of backcross progenies, makees male parent with 1 part of C01, carries out third time and returns
It hands over, obtains 50 parts of backcross progenies；
In July, 2015, Nanchang is female parent with 50 parts of backcross progenies, makees male parent with 1 part of C01, carries out the 4th time and returns
It hands over, obtains 100 parts of backcross progenies；
In March, 2016, Xining, Qinghai, backcross progeny are continuously selfed after 2 generations that obtain 100 parts of cabbage type rape definite inflorescences extensive
Multiple system.
The present embodiment additionally provides a kind of brassicacarinata and applies in the breeding of cabbage type rape definite inflorescence hybrid,
Selection specifically:
In July, 2016 keeps system for male parent, with 1 part of Wild cabbage type in Xining, Qinghai with 1 part of cabbage type rape definite inflorescence
Rape definite inflorescence sterile line is female parent, and hybridization obtains 10 parts of cabbage type rape definite inflorescence sterile line parents；In March, 2017,
It is female parent with 10 parts of cabbage type rape definite inflorescence sterile line parents in Nanchang, it is extensive with 1 part of cabbage type rape definite inflorescence
Multiple system is male parent, and hybridization obtains 50 parts of definite inflorescence hybrids.
Embodiment 4
In the Breeding Process of the present embodiment, daily light application time is 16h, when illumination deficiency, is irradiated using light, is controlled
Intensity of light processed is identical as natural light；
Present embodiments provide a kind of brassicacarinata answering in the breeding that cabbage type rape definite inflorescence keeps system
With, before planting, using mass fraction be 0.1% potassium permanganate solution Ethiopia's canola seed is impregnated, 4h
After dry, specifically:
In March, 2010 with 1 part of angstrom of mustard is female parent in Nanchang, with 1 part of cabbage type rape indefinite inflorescence strain (in it is double
11) it is male parent, is hybridized, therefrom screens the plant of definite inflorescence character, obtain 1 part of hybrid generation F1；
With 1 part of hybrid generation F1 it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out first in July, 2010
Secondary backcrossing obtains 5 parts of backcross progeny；
With 5 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out second in March, 2011
Backcrossing, obtains 10 parts of backcross progeny；
With 10 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out third time in July, 2011
Backcrossing, obtains 30 parts of backcross progeny；
With 30 parts of backcross progenies it is female parent in Nanchang, is male parent in 1 part double 11, carries out the 4th time in March, 2012
Backcrossing, obtains 50 parts of backcross progeny；
With 50 parts of backcross progenies it is female parent in Xining, Qinghai, is male parent in 1 part double 11, carries out the 5th time in July, 2012
Backcrossing, was then selfed for 1 generation, obtains 100 parts of cabbage type rape definite inflorescences and keeps system.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line
Using selection specifically:
In March, 2013, Nanchang keep system for male parent, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish backbone sterile line (RF02-004) is female parent, is hybridized, obtains 1 part of filial generation F2；
In July, 2013, Xining, Qinghai are that non-circulation is maternal with 1 part of F2, keep system with 1 part of cabbage type rape definite inflorescence
For circulation male parent, first time backcrossing is carried out, 20 parts of backcross progeny BC1 are obtained；
Female parent is made with 20 parts of BC1 in March, 2014, Nanchang, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
It carries out second to be returned, obtains 50 parts of backcross progeny BC2；
In July, 2014, Xining, Qinghai are female parent with 50 parts of BC2, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
Third time backcrossing is carried out, 100 parts of backcross progeny BC3 are obtained；
In March, 2015, Nanchang is female parent with 100 parts of BC3, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This progress the 4th time backcrossing, obtains 100 parts of backcross progeny BC4；
In July, 2015, Xining, Qinghai is female parent with 100 parts of BC4, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This, carries out the 5th backcrossing, obtains 100 parts of cabbage type rape definite inflorescence sterile lines.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer
Using selection specifically:
In March, 2013, Nanchang keep system to make maternal, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish indefinite inflorescence elite restorer lines (C01) make male parent, and hybridization obtains 1 part of hybrid generation F3；
In July, 2013, Nanchang are selfed to obtain 1 part of F4 with 1 part of F3；
In March, 2014, Xining, Qinghai are that non-circulation is maternal with 1 part of F4, using 1 part of C01 as circulation male parent, carry out the 1st time and return
It hands over, obtains 10 parts of backcross progenies；
In July, 2014, Nanchang is female parent with 10 parts of backcross progenies, makees male parent with 1 part of C01, carries out second time
It hands over, obtains 20 parts of backcross progenies；
In March, 2015, Xining, Qinghai is female parent with 20 parts of backcross progenies, makees male parent with 1 part of C01, carries out third time and returns
It hands over, obtains 50 parts of backcross progenies；
In July, 2015, Nanchang is female parent with 50 parts of backcross progenies, makees male parent with 1 part of C01, carries out the 4th time and returns
It hands over, obtains 100 parts of backcross progenies；
In March, 2016, Xining, Qinghai, backcross progeny are continuously selfed after 2 generations that obtain 100 parts of cabbage type rape definite inflorescences extensive
Multiple system.
The present embodiment additionally provides a kind of brassicacarinata and applies in the breeding of cabbage type rape definite inflorescence hybrid,
Selection specifically:
In July, 2016 keeps system for male parent, with 1 part of Wild cabbage type in Xining, Qinghai with 1 part of cabbage type rape definite inflorescence
Rape definite inflorescence sterile line is female parent, and hybridization obtains 10 parts of cabbage type rape definite inflorescence sterile line parents；In March, 2017,
It is female parent with 10 parts of cabbage type rape definite inflorescence sterile line parents in Nanchang, it is extensive with 1 part of cabbage type rape definite inflorescence
Multiple system is male parent, and hybridization obtains 50 parts of definite inflorescence hybrids.
Embodiment 5
All Breeding Process of the present embodiment carry out under field conditions (factors)；
Present embodiments provide a kind of brassicacarinata answering in the breeding that cabbage type rape definite inflorescence keeps system
With selection specifically:
In March, 2010, in Nanchang with 1 part of angstrom of mustard to be maternal, with 1 part of cabbage type rape (moral oil 5) for male parent, into
Row hybridization, therefrom screens the plant of definite inflorescence character, obtains 1 part of hybrid generation F1；
In July, 2010, with 1 part of moral oil 5 for male parent, carries out first in Xining, Qinghai with 1 part of hybrid generation F1 to be maternal
Secondary backcrossing obtains 5 parts of backcross progeny；
In March, 2011, with 1 part of moral oil 5 for male parent, carries out second in Nanchang with 5 parts of backcross progenies to be maternal
Backcrossing, obtains 10 parts of backcross progeny；
In July, 2011, with 1 part of moral oil 5 for male parent, carries out third in Xining, Qinghai with 10 parts of backcross progenies to be maternal
Secondary backcrossing obtains 30 parts of backcross progeny；
In March, 2012, with 1 part of moral oil 5 for male parent, carries out the 4th in Nanchang with 30 parts of backcross progenies to be maternal
Secondary backcrossing obtains 50 parts of backcross progeny；
In July, 2012, with 1 part of moral oil 5 for male parent, carries out the 5th in Xining, Qinghai with 50 parts of backcross progenies to be maternal
Then secondary backcrossing was selfed for 1 generation, obtain 100 parts of cabbage type rape definite inflorescences and keep system.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line
Using selection specifically:
In March, 2013, Nanchang keep system for male parent, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish backbone sterile line (17A) is female parent, is hybridized, obtains 1 part of filial generation F2；
In July, 2013, Xining, Qinghai are that non-circulation is maternal with 1 part of F2, keep system with 1 part of cabbage type rape definite inflorescence
For circulation male parent, first time backcrossing is carried out, 20 parts of backcross progeny BC1 are obtained；
Female parent is made with 20 parts of BC1 in March, 2014, Nanchang, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
It carries out second to be returned, obtains 50 parts of backcross progeny BC2；
In July, 2014, Xining, Qinghai are female parent with 50 parts of BC2, and 1 part of cabbage type rape definite inflorescence keeps system to make male parent,
Third time backcrossing is carried out, 100 parts of backcross progeny BC3 are obtained；
In March, 2015, Nanchang is female parent with 100 parts of BC3, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This progress the 4th time backcrossing, obtains 100 parts of backcross progeny BC4；
In July, 2015, Xining, Qinghai is female parent with 100 parts of BC4, keeps system to make father with 1 part of cabbage type rape definite inflorescence
This, carries out the 5th backcrossing, obtains 100 parts of cabbage type rape definite inflorescence sterile lines.
The present embodiment additionally provides a kind of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer
Using selection specifically:
In March, 2013, Nanchang keep system to make maternal, with 1 part of Wild cabbage type oil with 1 part of cabbage type rape definite inflorescence
Dish indefinite inflorescence elite restorer lines (HF16-1400R) make male parent, and hybridization obtains 1 part of hybrid generation F3；
In July, 2013, Nanchang are selfed to obtain 1 part of F4 with 1 part of F3；
In March, 2014, Xining, Qinghai are that non-circulation is maternal with 1 part of F4, using 1 part of HF16-1400R as circulation male parent, carry out
1st backcrossing, obtains 10 parts of backcross progenies；
In July, 2014, Nanchang is female parent with 10 parts of backcross progenies, makees male parent with 1 part of HF16-1400R, carries out the
Secondary backcrossing obtains 20 parts of backcross progenies；
In March, 2015, Xining, Qinghai is female parent with 20 parts of backcross progenies, makees male parent with 1 part of HF16-1400R, carries out the
It is returned three times, obtains 50 parts of backcross progenies；
In July, 2015, Nanchang is female parent with 50 parts of backcross progenies, makees male parent with 1 part of HF16-1400R, carries out the
Four backcrossings, obtain 100 parts of backcross progenies；
In March, 2016, Xining, Qinghai, backcross progeny are continuously selfed after 2 generations that obtain 100 parts of cabbage type rape definite inflorescences extensive
Multiple system.
The present invention also provides a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence hybrid,
Selection specifically:
In July, 2016 keeps system for male parent, with 1 part of Wild cabbage type in Xining, Qinghai with 1 part of cabbage type rape definite inflorescence
Rape definite inflorescence sterile line is female parent, and hybridization obtains 10 parts of cabbage type rape definite inflorescence sterile line parents；In March, 2017,
It is female parent with 10 parts of cabbage type rape definite inflorescence sterile line parents in Nanchang, it is extensive with 1 part of cabbage type rape definite inflorescence
Multiple system is male parent, and hybridization obtains 50 parts of definite inflorescence hybrids.
In July, 2017 obtains embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5 in Nanchang
Cabbage type rape definite inflorescence keep system, cabbage type rape definite inflorescence sterile line, cabbage type rape definite inflorescence restorer and
Definite inflorescence hybrid, totally 20 strains are planted in the Tanaka that area is 1 mu respectively；Wild cabbage type indefinite inflorescence strain is selected simultaneously
(RS013-008) as a comparison case, identical as definite inflorescence strain planting conditions.It is as follows through statistics, measurement result after harvest,
It is shown in Table 1.
The statistical measurements of table 1 embodiment 1-5 and comparative example
Above-mentioned statistical measurements explanation, compared with Wild cabbage type indefinite inflorescence strain, the flower of Wild cabbage type definite inflorescence strain
Phase can at most shorten 15 days, and main shaft and branch silique seed mass of 1000 kernel, single plant yield and yield significantly improve, and mechanical loss rate is most
5.9% can be mostly reduced, do mechanization operation is helped to realize；Invalid inflorescence and invalid silique is not present in definite inflorescence strain, preferably
Solve the problems, such as indefinite inflorescence increase Oilseed rape biomass effective consumption.
The sterile rate of cabbage type rape definite inflorescence sterile line is 100% in Breeding Process, the limited flower of cabbage type rape
The recovery rate of sequence restorer is 100%, and the purity of definite inflorescence hybrid is mentioned 95% or more for Rape-seed production popularization and application
Novel cabbage type rape definite inflorescence seed is supplied.
In addition, embodiment 1 is compared with embodiment 2, illustrate, helps to shorten flower by controlling illumination in Breeding Process
Phase；Embodiment 1 is compared with embodiment 3, is illustrated, carrying out Dressing to seed with potassium permanganate before planting in Breeding Process has
Help improve germination percentage；Embodiment 1 is compared with embodiment 4, is illustrated, by control illumination and to dressing seed in Breeding Process
Processing helps to shorten the florescence, improves the germination percentage of seed.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of application of brassicacarinata in the breeding that cabbage type rape definite inflorescence keeps system.
2. brassicacarinata according to claim 1 answering in the breeding that cabbage type rape definite inflorescence keeps system
With, which is characterized in that including,
Using cabbage type rape indefinite inflorescence as male parent, it is female parent with brassicacarinata, is hybridized, obtain hybrid generation F1；
It is that non-circulation is maternal with the hybrid generation F1, using the cabbage type rape indefinite inflorescence strain as male parent, continuous backcross,
Cabbage type rape definite inflorescence is obtained after selfing keeps system；
Wherein, the non-circulation female parent is the plant for showing as definite inflorescence character；
The backcrossing number is 4-5 times；The selfing number is 1 time.
3. brassicacarinata according to claim 1 answering in the breeding that cabbage type rape definite inflorescence keeps system
With in the Breeding Process, controlling daily light application time is 14h-16h.
4. brassicacarinata according to claim 1 answering in the breeding that cabbage type rape definite inflorescence keeps system
With in the Breeding Process, using potassium permanganate solution to Ethiopia's canola seed progress Dressing；The height
The mass fraction of potassium manganate is 0.1%-0.5%.
5. a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence sterile line.
6. brassicacarinata according to claim 5 answering in the breeding of cabbage type rape definite inflorescence sterile line
With, which is characterized in that including,
The cabbage type rape definite inflorescence obtained with breeding in any one of the claim 1-4 application keeps system for male parent, with
Cabbage type rape backbone sterile line is female parent, is hybridized, obtains F2；
It is female parent with the F2, keeps system for male parent with the cabbage type rape definite inflorescence, carry out first time backcrossing, returned
Hand over offspring BC1；
It is that non-circulation is maternal with BC1, keeps system for circulation male parent with the cabbage type rape definite inflorescence, continuous backcross obtains
Cabbage type rape definite inflorescence sterile line；
Wherein, the F2 is the plant for showing as definite inflorescence character and sterile character；
The BC1 is the plant for showing as definite inflorescence character and sterile character；
The number of the continuous backcross is 4-5 times.
7. a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence restorer.
8. brassicacarinata according to claim 7 answering in the breeding of cabbage type rape definite inflorescence restorer
With, which is characterized in that the selection specifically:
System is kept to make with the cabbage type rape definite inflorescence that breeding in any one of the claim 1-4 application obtains maternal, with
Cabbage type rape indefinite inflorescence elite restorer lines make male parent, and hybridization obtains hybrid generation F3；F3 obtains F4 after being selfed 1 time；
It is that non-circulation is maternal with the F4；Cabbage type rape indefinite inflorescence elite restorer lines are circulation male parent；The 1st time is carried out to return
It hands over, obtains backcross progeny；
It is that non-circulation is maternal with the backcross progeny；Circulation male parent is reverted to the cabbage type rape indefinite inflorescence backbone, even
Continuous backcrossing, selfing obtain cabbage type rape definite inflorescence restorer；
Wherein, it is khaki plant that the F3, which is pattern,；
The nonrecurrent parent is definite inflorescence character and pattern is khaki plant；
The backcrossing number is 4 or 5 times, and the selfing number is 2 times.
9. a kind of application of brassicacarinata in the breeding of cabbage type rape definite inflorescence hybrid.
10. application of the brassicacarinata according to claim 9 in the breeding of cabbage type rape definite inflorescence hybrid,
It is characterised in that it includes
The cabbage type rape definite inflorescence obtained with breeding in any one of the claim 1-4 application keeps system for male parent, with
The cabbage type rape definite inflorescence sterile line that breeding obtains in any one of the claim 5-6 application is female parent, and hybridization obtains
Cabbage type rape definite inflorescence sterile line parent；
It is female parent with the cabbage type rape definite inflorescence sterile line parent, to be selected in any one of the claim 7-8 application
The cabbage type rape definite inflorescence restorer educated is male parent, and hybridization obtains definite inflorescence hybrid.
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