CN104904587A - Method for selectively breeding cytoplasmic male sterile lines and maintainer lines of cabbage type rapes - Google Patents
Method for selectively breeding cytoplasmic male sterile lines and maintainer lines of cabbage type rapes Download PDFInfo
- Publication number
- CN104904587A CN104904587A CN201510298748.9A CN201510298748A CN104904587A CN 104904587 A CN104904587 A CN 104904587A CN 201510298748 A CN201510298748 A CN 201510298748A CN 104904587 A CN104904587 A CN 104904587A
- Authority
- CN
- China
- Prior art keywords
- line
- fertility
- maintainer
- bred
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for selectively breeding cytoplasmic male sterile lines and maintainer lines of cabbage type rapes. By means of the method disclosed by the invention, the problem that the maintenance effect of the maintainer lines selectively bred according to the traditional method is greatly reduced due to loss of sterile genes rf having steady and thorough fertility in the maintainer line testcross and screening process can be solved; and therefore, the cytoplasmic male sterile lines and the maintainer lines of the cabbage type rapes having steady fertility can be selectively bred. The method disclosed by the invention is high in operability, clear in theory and simple in selective breeding process; the selective breeding efficiency of the cytoplasmic male sterile lines is increased; and the fertility of the bred cytoplasmic male sterile lines is more thorough and steady.
Description
Technical field
The present invention relates to the selection of rape, particularly relate to the selection of a kind of cabbage type rape cytoplasmic male sterile line and maintainer thereof.
Background technology
Rape belongs to Cruciferae (Cruciferae) Brassicas (Brassica) plant, and be one of large oil crop in the world four, the main oil crop of Ye Shi China, occupy critical role in the national economy and people's lives of China.Napus hybrid advantage is remarkable, and generally can increase production 20-30%, therefore the utilization of rape heterosis is one of key areas of rapeseed breeding.Utilize rape heterosis approach the most general with the sterile system of matter in the world, the basis of seed selection matter sterility cross-bred rape kind is the seed selection of three based materials, and the stability of matter male sterile line sterility and the height of seed selection level directly have influence on cross-bred rape variety yield potentiality and production application area.
Two classical practical cabbage type rape (Brassic Napus L.AACC, 2n=4x=38) the discovery of cytoplasmic male sterile line Polima A and Shan 2A three more than ten years, these two famous male sterile lines China and even world's rape heterosis research and played important function in utilizing.Up to now, in the Rapeseed CMS three line hybrid seed of authorization both at home and abroad, great majority Pol CMS and Shan 2A CMS is formulated.Polima A teaches 1972 from originating in the sterile strain of natural mutation found the cabbage type rape variety Polima of Poland by Hua Zhong Agriculture University Fu Tingdong, be considered to first Rapeseed CMS (Fang and McVetty, 1989) with practical value in the world at present.Shan 2A finds also seed selection in 1976 by Shanxi Province Crossbred Cole Research Center Li Dianrong researcher from the filial generation of cabbage type rape variety S74-3 × (having a good harvest No. 4+7207), by cabbage type rape three line hybrid seed " No. two, Qin's oil " (the Shan 2A × cultivate C1 of its apolegamy, nineteen eighty-two be bred as) be in the world first by authorization and successfully large-area applications in produce cross-bred rape kind, achieve huge economic benefit and social benefit.
Cabbage type rape cytoplasmic male sterility is that rape heterosis utilizes the most extensively, the most effective approach, the rape male sterile line of most actual application value is Polima A and Shan 2A in the world, the advantage of two Iarge-scale system is that Restorer varieties is easy to find, be easy to realize three series mating, without bad cytoplasmic effect, sterility is relatively stable.Its major defect is the impact that the fertility of this two Iarge-scale system is subject to environmental factor, susceptibility is in various degree revealed to thermometer, namely under initial bloom stage cryogenic conditions, easily the even relatively large pollen of trace is produced, energy selfing or sisters' knot are in fact, thus affect the purity of crossbreed and heterotic performance, there is certain risk (Fu Tingdong and Yang Guangsheng, 1995) in production.The greatest problem of Polima A and Shan 2A is that male sterile line initial bloom stage chance low temperature can produce trace-pollen, and cause the self-fertility phenomenon of about 10%, affect the purity of parent and crossbreed, the special time can cause risk in production.
In recent years, for the micro mist problem of the large male sterile line of PolA and Shan 2A two, by the effort for many years of agri-scientific research technological staff, micro mist problem improves to a great extent.But all there is various defect in the micro mist being controlled male sterile line by adjustment date of seeding, the cultivation means such as top, adjustment row ratio, chemical emasculation or the strange land production of hybrid seeds of plucking, such as increase breeding and breeding cost, technical measures are not easy to operate, delayed the seed sale time adds the problems such as business capital turnover period from face, therefore limit applying of PolCMS and the large sterile series hybrid of Shan 2ACMS two to a certain extent.Breeding man is thought and fundamentally to be eliminated by genetic improvement or to alleviate the impact of micro mist, it is the most desirable, cost-effective method, by introducing cell nucleus sterile gene, screen and can reduce or eliminate micro mist to means such as temperature-insensitive gene maintainer, mutation breedings, and achieve certain effect, but still imperfection.Therefore, be only found new sterile material and adopt new selection just can cultivate the stable novel fine cytoplasm male sterility line of a kind of sterility, fundamentally solving current cytoplasmic male sterile line micro mist problem.
Summary of the invention
Technical problem to be solved by this invention is: the selection providing a kind of cabbage type rape cytoplasmic male sterile line and maintainer thereof, it can select cytoplasmic male sterile line and the maintainer thereof of stable fertility, reducing existing cytoplasmic male sterile line micro mist causes self-fertility to affect the problem of parent and purity of hybrid, and seed selection program is simple, workable.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme: the selection of cabbage type rape cytoplasmic male sterile line and maintainer thereof, comprises the steps:
(1) by the cytoplasmic male sterility material incubation thoroughly of fertility in cabbage type rape AB line: the fertile plant of the cytoplasmic male sterility strain thoroughly of newfound fertility and this plant is hybridized, F1, F2 are for bagging selfing, carrying out brother and sister's test cross at F3 for selecting to educate with sterile ratio the plant being 3:1, occurring that namely fertility is bred as fertility than the AB line for 1:1 than for brother and sister continuous in the plant of 1:1 hand in test cross combination;
(2) screen Fertile cytoplasm: with the fertile plant of the AB line be bred as in step (1) for parent sire of hybrid pigs, itself and crucifer are hybridized, what hybridization F2 generation was separated without sterile strain is Fertile cytoplasm;
(3) new maintainer is synthesized by the genic male sterile gene of matter sterile material importing Fertile cytoplasm: Fertile cytoplasm material step (2) screened is for maternal, hybridize with the AB line fertile plant be bred as in step (1), hybridize F1 bagging selfing or backcross, select the paired test cross of the sterile strain of AB line be bred as in the individual plant of F2 generation or BC1F1 and step (1), occur that the test cross individual plant of the combination correspondence of complete sterility is the maintainer N of new synthesis test cross combination offspring, namely fertility must be equally thorough with the sterile material in cabbage type rape in step (1), stable;
(4) cytoplasmic male sterile line of stable fertility is bred as: the sterile strain continuous backcross of AB line that the maintainer N synthesize step (3) and step (1) are bred as, is namely bred as the neoplasm male sterile line of stable fertility.
Crucifer described in step (2) comprises that brassicacarinata is cytoplasmic cabbage type rape, white oil dish is cytoplasmic cabbage type rape.
Compared with the prior art, the present invention has the following advantages: (1), traditional cabbage type rape cytoplasmic sterility system selective breeding method are with the sterile strain of newfound matter and a large amount of testcross and screening maintainer of conventional variety (being), be difficult to screen and keep maintainer satisfactory for result, because in the process of conventional method screening maintainer, do not consider that the genic male sterile gene rf of newfound cytoplasmic male sterility material is on the impact of fertility stability, thus cause existing cabbage type rape cytoplasmic male sterile line (Polima A and Shan 2A) fertility by the impact of temperature; (2), selection of the present invention avoids conventional method in the process of testcross and screening maintainer, stable fertility, thoroughly sterile gene rf are lost, the problem causing the maintainer of traditionally seed selection maintenance effect greatly to reduce, therefore can select cytoplasmic male sterile line and the maintainer thereof of stable fertility.The inventive method is workable, and principle is clear, and seed selection program is simple, improves the Breeding Efficiency of cytoplasmic male sterile line, and the cytoplasmic male sterile line fertility of incubation is more thorough, stable.
Embodiment
Embodiments of the invention 1: the selection of cabbage type rape cytoplasmic male sterile line and maintainer thereof (be in cytoplasmic cabbage type rape screen Fertile cytoplasm and synthesis new keep be example with brassicacarinata).
In March, (1) 2008 finds 1 strain sterility cytoplasmic male sterility strain thoroughly in conventional strain 891 plant of cabbage type rape, namely hybridize with the fertile plant in this plant and sterile strain then, hybridization F1 selfing, F2 select fertile plant selfing, be with the paired brother and sister's test cross of Sterile plants and fertile plants in the plant of 3:1 at F3 for Fertility segregation, continue brother and sister's test cross at the test cross combination offspring Fertility segregation plant that is 1:1 and be namely bred as fertile plant S(Rfrf) with sterile strain S(rfrf) ratio be the AB line 891AB of the sterile strain stable fertility of 1:1;
(2) the fertile plant S(Rfrf of the AB line 891AB be bred as with step (1)) for male parent, hybridize with multiple normal rapeseed kind (being), F1 selfing, the kind (being) of the combination correspondence that F2 generation is separated without sterile strain is Fertile cytoplasm N(_ _), there is sterile cytoplasm S(N that sterile strain is separated (_ _) in superseded F2) combine.
(3) in step (2), with the new cabbage type rape strain that conventional strain 5771(5771 is with brassicacarinata and cabbage type rape Prof. Du Yucang, cytoplasm for brassicacarinata) be that maternal hybrid combination F2 is separated without sterile strain, the paired test cross of sterile strain of the 891AB selecting this combination F2 individual plant and step (1) to be bred as, 5 paired test cross combinations are had to occur complete sterility, the test cross individual plant of complete sterility combination correspondence is maintainer N(rfrf), continue to combine sterile strain continuous backcross by this maintainer and complete sterility and be namely bred as the cytoplasmic male sterile line 9171A of stable fertility.
Embodiments of the invention 2: the selection of cabbage type rape cytoplasmatic male maintainer and maintainer thereof (be in cytoplasmic cabbage type rape screen Fertile cytoplasm and synthesis new keep be example with white oil dish).
(1), (2) are identical with embodiment 1 step.
(3) substantially identical with (3) step of embodiment 1, unlike at conventional strain 5602(5602 being the new cabbage type rape strain using white oil dish and cabbage type rape Prof. Du Yucang, cytoplasm for white oil dish) for maternal hybridization F2 is for being separated without sterile strain, at the paired test cross of sterile strain of the 891AB that its F2 menu strain and step (1) are bred as, 3 paired test cross combinations are had to occur complete sterility, the test cross individual plant of complete sterility combination correspondence is maintainer N(rfrf), continue to combine sterile strain continuous backcross by this maintainer and complete sterility and be namely bred as the cytoplasmic male sterile line 9102A of stable fertility.
In order to verify technique effect of the present invention, applicant in September, 2012,2013,2014 respectively by 9171A, 9102A of being bred as in embodiment 1 and embodiment 2 with plant at Sinan County pool head (height above sea level 380 meters), Meitan County prosperity (height above sea level 850 meters), Kweiyang (height above sea level 1150 meters) respectively with the male sterile line that conventional method is bred as, to identify that the male sterile line of different selection seed selection is at Different climate condition, fertility stability between difference year flowering stage in the rape in 2 ~ March of next year.
Result proves, it is thorough, stable that male sterile line 9171A, 9102A that embodiment 1 and embodiment 2 obtain all show as sterility under Different climate condition, between different year; The fertility performance of the male sterile line be bred as by conventional method is unstable, differs greatly between different year.
Claims (2)
1. a selection for cabbage type rape cytoplasmic male sterile line and maintainer thereof, is characterized in that: comprise the steps:
(1) by the cytoplasmic male sterility material incubation thoroughly of fertility in cabbage type rape AB line: hybridize by the fertile plant of the cytoplasmic male sterility strain thoroughly of newfound fertility and this plant, F1, F2 are for bagging selfing, carrying out brother and sister's test cross at F3 for selecting the plant that fertile plant and the ratio of sterile strain are 3:1, occurring that namely fertility is bred as fertility than the AB line for 1:1 than for brother and sister continuous in the plant of 1:1 hand in test cross combination;
(2) screen Fertile cytoplasm: with the fertile plant of the AB line be bred as in step (1) for parent sire of hybrid pigs, itself and crucifer are hybridized, what hybridization F2 generation was separated without sterile strain is Fertile cytoplasm;
(3) new maintainer is synthesized by the genic male sterile gene of matter sterile material importing Fertile cytoplasm: the Fertile cytoplasm material screened by step (2) is for maternal, hybridize with the AB line fertile plant be bred as in step (1), hybridize F1 bagging selfing or backcross, select the paired test cross of the sterile strain of AB line be bred as in the individual plant of F2 generation or BC1F1 and step (1), occur that the test cross individual plant of the combination correspondence of complete sterility is the maintainer N (rfrf) of new synthesis test cross combination offspring, namely fertility must be equally thorough with newfound sterile material in step (1), stable;
(4) be bred as the cytoplasmic male sterile line of stable fertility: the sterile strain continuous backcross of AB line that the maintainer N (rfrf) step (3) synthesized and step (1) are bred as, be namely bred as the neoplasm male sterile line of stable fertility.
2. the selection of cabbage type rape cytoplasmic male sterile line according to claim 1 and maintainer thereof, is characterized in that: it be cytoplasmic cabbage type rape or white oil dish is cytoplasmic cabbage type rape that the crucifer described in step (2) comprises brassicacarinata.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510298748.9A CN104904587B (en) | 2015-06-03 | 2015-06-03 | The selection of cabbage type rape cytoplasmic male sterile line and its maintainer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510298748.9A CN104904587B (en) | 2015-06-03 | 2015-06-03 | The selection of cabbage type rape cytoplasmic male sterile line and its maintainer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104904587A true CN104904587A (en) | 2015-09-16 |
| CN104904587B CN104904587B (en) | 2018-07-17 |
Family
ID=54074385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510298748.9A Expired - Fee Related CN104904587B (en) | 2015-06-03 | 2015-06-03 | The selection of cabbage type rape cytoplasmic male sterile line and its maintainer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104904587B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168421A (en) * | 2018-10-09 | 2019-01-11 | 江西省农业科学院作物研究所 | Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002355041A (en) * | 2001-04-25 | 2002-12-10 | Mitsubishi Chemicals Corp | Gene relating to restoration from cytoplasmic male sterility to fertility |
| GB2429462A (en) * | 2005-08-23 | 2007-02-28 | Elsoms Seeds Ltd | Male sterile swede plants and F1 hybrids |
| CN101390493A (en) * | 2008-10-24 | 2009-03-25 | 西北农林科技大学 | Breeding method of cabbage type rape dual sterile line |
| CN101960984A (en) * | 2009-07-23 | 2011-02-02 | 广东海洋大学 | Method for improving fertility stability of brassica napus L. cytoplasmic sterile line |
| CN104429918A (en) * | 2014-08-07 | 2015-03-25 | 贵州省油菜研究所 | Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes |
-
2015
- 2015-06-03 CN CN201510298748.9A patent/CN104904587B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002355041A (en) * | 2001-04-25 | 2002-12-10 | Mitsubishi Chemicals Corp | Gene relating to restoration from cytoplasmic male sterility to fertility |
| GB2429462A (en) * | 2005-08-23 | 2007-02-28 | Elsoms Seeds Ltd | Male sterile swede plants and F1 hybrids |
| CN101390493A (en) * | 2008-10-24 | 2009-03-25 | 西北农林科技大学 | Breeding method of cabbage type rape dual sterile line |
| CN101960984A (en) * | 2009-07-23 | 2011-02-02 | 广东海洋大学 | Method for improving fertility stability of brassica napus L. cytoplasmic sterile line |
| CN104429918A (en) * | 2014-08-07 | 2015-03-25 | 贵州省油菜研究所 | Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes |
Non-Patent Citations (6)
| Title |
|---|
| 席代汶: "甘蓝型油菜玻里马胞质雄性不育系微量花粉的成因及改良", 《作物研究》 * |
| 张瑞茂等: "甘蓝型油菜黄籽双低隐性核不育两系86AB的选育", 《种子》 * |
| 潘涛等: "甘蓝型双低油菜雄性核不育两用系的选育研究", 《作物研究》 * |
| 蔡旭: "《植物遗传育种学（第二版）》", 31 December 1988, 科学出版社 * |
| 陈大伦等: "甘蓝型油菜隐性上位细胞核雄性不育三系的转育方法", 《贵州农业科学》 * |
| 陈大伦等: "甘蓝型油菜黄籽双低细胞质雄性不育系RM 3231A及保持系RM 3231B的选育", 《种子》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168421A (en) * | 2018-10-09 | 2019-01-11 | 江西省农业科学院作物研究所 | Application of the brassicacarinata in the breeding of cabbage type rape definite inflorescence strain |
| CN109168421B (en) * | 2018-10-09 | 2021-06-08 | 江西省农业科学院作物研究所 | Application of Arabidopsis thaliana in breeding of brassica napus limited inflorescence strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104904587B (en) | 2018-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ye et al. | Seedless mechanism of a new mandarin cultivar ‘Wuzishatangju’(Citrus reticulata Blanco) | |
| CN104137765A (en) | Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line | |
| Wen et al. | Characterization of interploid hybrids from crosses between Brassica juncea and B. oleracea and the production of yellow-seeded B. napus | |
| Rehman et al. | CRISPR gene editing of major domestication traits accelerating breeding for Solanaceae crops improvement | |
| CN104488699B (en) | The selection of erect head long stigma long fringe neck two-line sterile line | |
| CN105145334A (en) | Seed selection method of new rice species | |
| CN104620880B (en) | Promote that temperature sensitive Genetic Sterility Semen Tritici aestivi BNS keeps the chemical regulation method that line fertility recovers | |
| CN104904587A (en) | Method for selectively breeding cytoplasmic male sterile lines and maintainer lines of cabbage type rapes | |
| CN103348908B (en) | Method for selecting hybrid seeds of Brassica juncea var. multiceps | |
| CN103120118B (en) | Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line | |
| CN104115754B (en) | A kind of selection with the radish maintainer of Ogura male sterile cytoplasm | |
| Silva Ferreira et al. | BIFURCATE FLOWER TRUSS: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene | |
| CN102150613A (en) | Method for breeding cytoplasm male sterile line of novel cabbage type rape | |
| CN1994059A (en) | Three-step method for purification of Dian-1 type japonica sterile line stock seed | |
| CN113080053B (en) | Method for improving wheat male sterile line and application thereof | |
| CN105794509A (en) | SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate | |
| LIU et al. | Experimental and genomic evidence for the indica-type cytoplasmic effect in Oryza sativa L. ssp. japonica | |
| CN109168421B (en) | Application of Arabidopsis thaliana in breeding of brassica napus limited inflorescence strain | |
| CN106900545A (en) | A kind of selection of indica hybrid rice CMS line | |
| CN102919116B (en) | Selection breeding method of new homozygous sterile line of brassica napus | |
| CN102742496A (en) | Rapid transfer improvement method for rape recessive homozygosis sterile line | |
| CN105830914A (en) | Breeding method of cabbage-type rape high oil-content recessive epistatic interaction nuclear sterile homozygous two-type line | |
| CN106688873A (en) | Cenxi soft-branch camellia oleifera bi-clonal seed orchard parent selection method | |
| CN112586338A (en) | Breeding method of japonica rice sterile line in early flowering | |
| CN104585019A (en) | Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180717 Termination date: 20200603 |